CN104956976B - A kind of artificial fecundation method of Carpinus tientaiensis - Google Patents
A kind of artificial fecundation method of Carpinus tientaiensis Download PDFInfo
- Publication number
- CN104956976B CN104956976B CN201510261102.3A CN201510261102A CN104956976B CN 104956976 B CN104956976 B CN 104956976B CN 201510261102 A CN201510261102 A CN 201510261102A CN 104956976 B CN104956976 B CN 104956976B
- Authority
- CN
- China
- Prior art keywords
- carpinus
- tientaiensis
- seed
- culture medium
- hours
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of artificial fecundation method of Carpinus tientaiensis, belong to plant artificial propagation techniques field, described method is comprised the following steps：（1）, pluck seed,（2）, storage,（3）, the broken dormancy of seed,（4）, selection soil culture medium nursery,（5）, Nursery stock transplanting, sapling multiplication is carried out to Carpinus tientaiensis seed using the present invention, Carpinus tientaiensis kind embryo after-ripening hibernation feature can be broken, make the quick breaking dormancy rudiment of seed and seedling, the germination rate of seed can be made to reach more than 50%, planting percent reaches more than 80%, the present invention is conducive to expanding Carpinus tientaiensis population quantity, for the extinction for preventing endangered plants Carpinus tientaiensis, both with important scientific meaning, and with important practice significance.
Description
Technical field
The invention belongs to plant artificial propagation techniques field, a kind of artificial propagation side of Carpinus tientaiensis is particularly related to
Method.
Background technology
Carpinus tientaiensis（Carpinus tientaiensis Cheng）, belong to Betulaceae（Betulaceae）, carpinus turczaninowii
Category（Carpinus）, deciduous tree is born in the mountain forest of 800~1000m of height above sea level, due to the serious fragmentation and breeding in habitat
Ability is weaker, and Carpinus tientaiensis is just endangered, is distributed only over Tiantai County Mount Huading and Panan County is winding greatly, only deposits 21 plants, point
The hm of cloth area about 0.32, in pole danger state, less than Wild population stabilization tolerance range, belong to minimum population wild plant,
It is listed in national two grades of Top-rated protected wild plants；Carpinus tientaiensis is Zhejiang endemic species, for the ground for studying Betulaceae
The aspects such as reason distribution, flora and bio-diversity have scientific research value higher.The tree-like grace of Carpinus tientaiensis seeds, sees
Reward property is strong, is the first-selected seeds of national natural reserves, National forest park Germ-plasma resources protection and flowers market ornamental plant.
Carpinus tientaiensis due to the defect of propagating system, the specialization of phytoecology characteristic only rely upon special environment,
Habitat, so causing population rare.Carpinus tientaiensis belongs to the seed of comprehensive dormancy, ripe Carpinus tientaiensis seed embryo
Structural integrity, but there is certain after ripening, hinder to the protection of rare plant Carpinus tientaiensis and to wild plant
Exploitation, is unfavorable for amount reproduction and the application of Carpinus tientaiensis plant.Carpinus tientaiensis loses competitive advantage at itself, and enclose the land guarantor
In the case of shield, it is also difficult to which natural regeneration is multiplied, it is necessary to carry out studying Carpinus tientaiensis propagation method, carried out by manual method
Breeding, moves to the nursery stock of reproductive success than in convenient or artificial creation corresponding conditionses, expanding population quantity, prevents from being on the point of
The extinction of danger plant Carpinus tientaiensis, both with important scientific meaning, and with important practice significance.But to being at present
Only, both at home and abroad for the also no effective method of the artificial breeding of Carpinus tientaiensis.
The content of the invention
The present invention is to be difficult breeding and at present artificial side in its natural state to solve endangered plants Carpinus tientaiensis
Method is difficult the technical problem of reproductive success, there is provided a kind of artificial fecundation method of Carpinus tientaiensis, using Carpinus tientaiensis seed
Sapling multiplication is carried out, seed shifts to an earlier date breaking dormancy, and the germination rate of seed reaches more than 50%, and planting percent reaches more than 80%.
A kind of artificial fecundation method of Carpinus tientaiensis provided by the present invention, comprises the following steps：
（1）, pluck seed
Carpinus tientaiensis mature seed is plucked, plucking time is annual 10 middle of the month to early November；
（2）, storage
The Carpinus tientaiensis mature seed that will be gathered, is stored in indoor temperature for 10 DEG C~25 DEG C, ventilation, dry
Side, the holding time is 20 days~150 days；
（3）, the broken dormancy of seed
The seed learnt from else's experience after preserving, washes away empty grain, and seed is put into thimerosal carries out disinfection treatment, then uses distilled water
Rinse well；Seed is placed in 20 DEG C~30 DEG C distilled water is again soaked 36~60 hours；
The seed after sterilization is placed in plant hormone is again soaked, the concentration of described plant hormone be set to 50mg/L~
200mg/L；
Seed after soaking, it is clean with distilled water flushing, seed is put into germination basin；Described germination basin is put into illumination
Incubator germinates, and the temperature setting of described germination basin is 20 DEG C~30 DEG C, and intensity of illumination is 1300 lx~1800 lx, light
The dark cycle is 12h: 12h；
（4）, selection soil culture medium nursery
The Carpinus tientaiensis seed plantation that radicle is sprouted is positive to the cultivation box or flowerpot or intelligence for filling soil culture medium
In light room, described soil culture medium proportioning is high mountain fallen leaves humus soil: river sand=1.5~3: 0.6~1.2, the soil
The pH of culture medium is 5.3~5.8, and cultivation box is put into phjytotron, and temperature setting is 18 DEG C~22 DEG C, humidity is 70~
80%, intensity of illumination is 3000lx~3500lx, and light dark period is 12h:12h, soil culture medium water-holding capacity is set to 35~
50%, treat that second true leaf grows, phjytotron temperature setting is 20 DEG C~28 DEG C, and humidity is set to 65~75%, illumination
Intensity is set to 7300 lx~8000 lx, and light dark period is set to 12h: 12h；
（5）, Nursery stock transplanting
After 3~5 true leaves are grown, nursery stock is moved on into even a film greenhouse, described company film greenhouse day temperature
20 DEG C~26 DEG C are set to, nocturnal temperature is set to 10 DEG C~15 DEG C, and humidity is 65~75%, and intensity of illumination is 10000 lx
~15000 lx, light dark period is set to 12h: 12h.
As a preferred scheme of the invention, in described step（2）In, the preservation temperature of Carpinus tientaiensis mature seed
It is 19 DEG C~21 DEG C to spend, and the described holding time is 30~40 days.
As a preferred scheme of the invention, described step（3）In thimerosal for mass fraction be 10% chlorine
Acid sodium solution, disinfecting time is set to 20 min~40min, then clean with distilled water flushing.
As another preferred scheme of the invention, described step（3）In thimerosal be H that mass fraction is 3%2O2
Solution, disinfecting time is set to 20 min~30min, then clean with distilled water flushing.
As a preferred scheme of the invention, a kind of artificial fecundation method of above-mentioned Carpinus tientaiensis, described step
Suddenly（3）In plant hormone be set to NAA, the concentration of described NAA is set to 50mg/L~200mg/L, at described immersion
Reason set of time is 18~30 hours.
Used as another preferred scheme of the invention, a kind of artificial fecundation method of above-mentioned Carpinus tientaiensis is described
Step（3）In plant hormone be set to GA3, described GA3Concentration be set to 50mg~200mg/L, at described immersion
Reason set of time is 36~60 hours.
Used as another preferred scheme of the invention, a kind of artificial fecundation method of above-mentioned Carpinus tientaiensis is described
Step（3）In plant hormone be set to 6-BA, the concentration of described 6-BA is set to 50mg~150mg/L, described immersion
Process time is set to 36~60 hours.
Used as another preferred scheme of the invention, a kind of artificial fecundation method of above-mentioned Carpinus tientaiensis is described
Step（3）In plant hormone be set to NAA and 6-BA, the concentration of described NAA is set to 50mg/L, described immersion treatment
Time is 20~28 hours；The concentration of described 6-BA is set to 50mg~200mg/L, and described immersion treatment set of time is
45~50 hours.
Used as another preferred scheme of the invention, a kind of artificial fecundation method of above-mentioned Carpinus tientaiensis is described
Step（3）In plant hormone be set to NAA and GA3, the concentration of described NAA is set to 50mg/L, described immersion treatment
Time is 24 hours；Described GA3Concentration be set to 50mg~200mg/L, described immersion treatment set of time for 45~
50 hours.
Used as another preferred scheme of the invention, a kind of artificial fecundation method of above-mentioned Carpinus tientaiensis is described
Step（4）In, described soil culture medium proportioning is high mountain fallen leaves humus soil: river sand=2: 1, the pH of the soil culture medium
It is 5.6 to be worth, and the temperature setting of described phjytotron is 20 DEG C, and humidity is set to 75%, intensity of illumination be 3000 lx~
3200 lx, light dark period is 12h:12h, soil culture medium water-holding capacity is controlled 35~50%, treats that second true leaf grows,
Phjytotron temperature setting is 25 DEG C, and humidity is set to 70%.
A kind of beneficial effect of the artificial fecundation method of Carpinus tientaiensis of the present invention is.
（1）, the present invention according to Carpinus tientaiensis seed germination, break Carpinus tientaiensis kind embryo after-ripening dormancy
Characteristic, manual simulation is carried out to its illumination of growing environment Main Factors, temperature, moisture content and growth hormone, seed is quickly broken
Dormancy rudiment and seedling, are successfully realized Carpinus tientaiensis artificial propagation.
（2）, the feature of more resistance to shade is shown according to Carpinus tientaiensis impeller structure, when cotyledon and true leaf are sprouted, by control
Illumination condition processed, appropriate shelters from heat or light, and makes seedling smooth growth.
（3）, the artificial fecundation method of Carpinus tientaiensis that is carried out using the present invention, Carpinus tientaiensis germination can be made
Rate reaches more than 50%, and the wherein survival rate of seedling is to more than 80%.
（4）, the artificial fecundation method of Carpinus tientaiensis that is carried out using the present invention, the nursery stock of reproductive success is moved to ratio
In convenient natural environment or artificial creation with corresponding conditionses environment in, be conducive to expanding population quantity, for preventing from being on the point of
The extinction of danger plant Carpinus tientaiensis, both with important scientific meaning, and with important practice significance.
Brief description of the drawings
Fig. 1 is a kind of artificial fecundation method flow chart of Carpinus tientaiensis of the invention.
Specific embodiment
The present invention is described in further detail for explanation below in conjunction with the accompanying drawings.
As shown in figure 1, a kind of artificial fecundation method of Carpinus tientaiensis of the present invention, comprises the following steps：Pluck
Seed, storage, the broken dormancy of seed, selection soil culture medium nursery and Nursery stock transplanting.
（1）, pluck seed, pluck Carpinus tientaiensis mature seed, through research, different time pluck mature seed, warp
Crossing same procedure carries out artificial propagation, but the germination rate of seed has very big difference, and optimum collecting time is annual 10 middle of the month
To early November.
（2）, storage, the Carpinus tientaiensis mature seed that will be gathered is stored in indoor temperature for 10 DEG C~25 DEG C, ventilation,
Dry place, the holding time is 20 days~150 days；Through research, the mature seed plucked simultaneously, identical ground is stored in
Side, but the duration for preserving is different, and artificial propagation is carried out by same procedure, but the germination rate of seed has very big difference,
If the holding time is long or too short, the germination rate of seed can be all influenceed, for the seed of Carpinus tientaiensis, be sent out by research
Existing, the holding time is 30 days or so, the germination rate highest of seed,（Referring to table 1）, such as in embodiment 1, in other incubations
In the case of identical, storage time is 30 days, and percentage of seedgermination is 50%, and survival rate of seedling is more than 80%；Storage time is 150
My god, percentage of seedgermination is 32%, slow 20 days of seedling-growing time, survival rate of seedling 53.5%；In the case where storage time is 150 days,
Preserved using sand storage mode, preserved 150 days in the case where water-holding capacity is 30%, other incubations find to plant with embodiment 1
Sub- germination percentage is 35%, survival rate of seedling 65%；Preserved 150 days in the case where water-holding capacity is 50%, other incubations are with implementation
Example 1, it is found that percentage of seedgermination is 5%, survival rate of seedling 52%；
（Table 1）The influence of different disposal and storage time to Carpinus tientaiensis percentage of seedgermination.
（3）, the broken dormancy of seed
Seed dormancy refers to seed living in suitable sprouting condition（Temperature, moisture and oxygen etc.）Under it is still not germinable existing
As；Seed dormancy is one of important adaptive character of plant.The reason for seed dormancy, can be classified as two major classes：The first kind be embryo in itself
Factor cause, including embryonic development is not completed；Physiologically prematurity；Lack necessary hormone or there is the thing of Inhibiting germination
Matter.Equations of The Second Kind is kind of a shell（Plant skin and pericarp etc.）Limitation cause.It is mechanical obstruction, impermeability including kind of shell, airtight
Property and plant shell in there is the reasons such as the material of Inhibiting germination.
Generally with breaking or the method for breaking dormancy has physical treatment process and method of chemical treatment, described physical treatment process bag
Include Temperature Treatment and mechanical treatment；Described method of chemical treatment includes gas treating process, inorganic chemistry drug-treated method, organises
Learn drug-treated method and Plant hormone treatment method；Described gas treating process as with 2.5% carbon dioxide, it is possible to by dormancy
Break；Described inorganic chemistry drug-treated method is exactly, using solution seed soakings such as inorganic chemistry pharmaceutical acid, alkali, to make the kind skin of seed
Corrosion cracking, ventilative water permeability enhancing makes to act on derepression with the inhibiting substances in seed, so as to break seed dormancy, promotees
Enter fast-germination；Described organic chemistry drug-treated method can use thiocarbamide, formaldehyde, dichloromethane, hydroquinones, colchicum
Essence, tartaric acid, oxyammonia etc. process seed, make seed Dormancy breaking, promote to sprout；Described Plant hormone treatment method is for example with red
Mycin is processed, and gibberellin is a kind of wide spectrum, efficient plant growth regulator, and seed can be made to do sth. in advance to terminate dormancy, improves hair
Bud rate, gibberellin also has stratification and shoot and leaf growth and the effect yielded positive results ahead of time to plant.Gibberellin is reversible de-
The effect that the acid that falls causes, breaks the dormancy induced by the latter；The effect of gibberellin stratification is especially apparent under cryogenic.
Through research, dense H is used2SO4, dilute HNO3, mechanical damage, warm water treatment, can not all break or release Carpinus tientaiensis
The dormancy of seed, it is impossible to sprout the seed of Carpinus tientaiensis（Referring to table 2）；
（Table 2）Dense H2SO4, dilute HNO3, mechanical damage, warm water influence of the treatment to Carpinus tientaiensis percentage of seedgermination.
Found through lot of experiments, can break or release stopping for Carpinus tientaiensis seed using appropriate plant hormone
Sleep, promote the seed of Carpinus tientaiensis to sprout, method is as follows：The seed learnt from else's experience after preserving, washes away empty grain, seed is put into and is disappeared
Venom carries out disinfection treatment, and described thimerosal is 10% liquor natrii hypochloritis for mass fraction, and disinfecting time is set to 20
Min~40min, then distilled water flushing is clean, then seed is placed in 20 DEG C~30 DEG C distilled water soaks 36~60 hours；Make
It is another embodiment, it is also possible to the H that mass fraction is 3%2O2Solution, disinfecting time is set to 20 min, then distills
Water is rinsed well, then seed is placed in 20 DEG C~30 DEG C distilled water soaks 36~60 hours.
The seed after sterilization is placed in plant hormone is again soaked, described plant hormone uses 6-BA, GA3, NAA or
NAA is combined with 6-BA, NAA and GA3Combination carries out treatment seed, can effectively facilitate the sprouting of Carpinus tientaiensis seed, wherein 6-
BA is 6-benzyladenine, cytokinin；GA3It is gibberellin 3；NAA is methyl α-naphthyl acetate, is artificial synthetic hormone class；Enter one
Step research discovery, 6-BA, GA3, NAA, NAA combined with 6-BA, NAA and GA3The various concentrations of combination, to the kind of Carpinus tientaiensis
Sub- germination percentage also has a great impact effect；When the concentration of described plant hormone is set to 50mg/L~200mg/L, with bright
Aobvious facilitation；When the excessive concentration of described plant hormone, seed cannot germinate.Swashed using a certain plant when single
When plain, different plant hormones and the various concentrations of different plant hormones are produced not to the percentage of seedgermination of Carpinus tientaiensis
Same effect；Combined with 6-BA and NAA and GA when using different NAA3During combination, the kind of different concentration to Carpinus tientaiensis
Sub- germination percentage can also produce different effects；As advantageous measure of the invention, as use NAA and GA3Combination, after sterilization
Seed is firstly placed on soaking in NAA being placed on GA again3Middle immersion, the concentration of described NAA is set to 50mg/L, and the immersion treatment time is
24 hours, described GA3Concentration be set to 50mg/L~200mg/L, the immersion treatment time is 48 hours, it is soaking after kind
Son, it is clean with distilled water flushing, seed is put into germination basin；Described germination basin is put into illumination box germination, described hair
The temperature setting of bud basin is 20~30 DEG C, and intensity of illumination is 1500 lx, and light dark period is 12h: 12h；By 8~11 days embryos
Root will be sprouted, and seed is successfully broken dormancy, and in this manner, Carpinus tientaiensis percentage of seedgermination reaches 50%,（Referring to table
3）；
（Table 3） 6-BA、GA3, NAA influences of the treatment to Carpinus tientaiensis percentage of seedgermination.
（4）, selection soil culture medium nursery
It has been investigated that, in the case of other condition of culture identicals, children of the different soils culture medium to Carpinus tientaiensis
The influence of seedling survival rate is larger, and soil culture medium proportioning has a strong impact on the growth of Carpinus tientaiensis seedling.Such as with high mountain yellow soil,
Seedling soil culture medium is done in chicken manure fertilizer and the mixing of fresh water river sand, Carpinus tientaiensis seedling can it is withered and yellow, wither Yan, survival rate of seedling is
0%；Such as mixed with border on the sea mud field soil, organic fertilizer and fresh water river sand and do seedling soil culture medium, because border on the sea mud field soil pH value is inclined
Height, is 6.5~7.0, and soil salt content is high, is constituted based on sodium chloride, mixes with organic fertilizer, fresh water river sand and does the training of seedling soil
Base is supported, Carpinus tientaiensis seedling occurs Yan that withers, death, and survival rate of seedling is 0%；Such as use high mountain yellow soil, organic fertilizer and river sand
Do seedling soil culture medium, due to the high mountain yellow soil content of organic matter it is low, it is necessary to plus a certain amount of organic fertilizer, increase N, P, K
Content, its survival rate of seedling is 50% or so.Such as with high mountain fallen leaves humus soil and river sand, because high mountain fallen leaves humus soil is protected
Water, permeable and venting capability are all relatively good, are conducive to the growth of seedling；As advantageous measure of the invention, high mountain fallen leaves humic
When matter soil is set to 2: 1 with the proportioning of river sand, pH value is 5.6, the g/kg of the content of organic matter 22~26, and its survival rate of seedling is maximum,
Survival rate of seedling is more than 80%.（Referring to table 4）；
（Table 4）Influence of the different soils culture medium to the survival rate of seedling of Carpinus tientaiensis.
During the region between the heart and the diaphragm seedling, the Carpinus tientaiensis seed plantation that radicle is sprouted to the cultivation box for filling soil culture medium, soil training
It is high mountain fallen leaves humus soil: river sand=2 to support basigamy ratio: 1, pH value is 5.6, the g/kg of the content of organic matter 22~26, plantation
Case is put into phjytotron, sets 18 DEG C~22 DEG C of constant temperature, and humidity is 70~80%, and intensity of illumination is 3150 lx, light dark period
It is 12h:12h, soil culture medium water-holding capacity is controlled 35~50%, and so, 5~7 days cotyledons of Carpinus tientaiensis seed are just
Can be unearthed, rough leaf grows within 10~15 days or so, second true leaf will grow after 20~25 days, treat that second true leaf is long
After going out, the temperature setting of phjytotron is 20 DEG C~28 DEG C of constant temperature, and humidity is 70%, and it is 7600 lx, brightness week that illumination increases
Phase is set to 12h: 12h；Thus favorably there is the growth of nursery stock.
（5）, Nursery stock transplanting
It has been investigated that, by after the treatment of identical method, being transplanted in the period of different, for survival rate of seedling
Have a great impact.Such as transplanted when seed is not sprouted, survival rate of seedling is 48%；Carried out after 2~3 true leaves are grown
Transplant, survival rate of seedling is more than 80%；Transplanted when 4~5 true leaves are grown, survival rate of seedling is 50%；Institute is as this
Nursery stock, an even film is moved on to after 2~3 true leaves are grown by the preferred embodiment of invention, and effect is best, survival rate of seedling highest.
（Referring to table 5）
（Table 5）Influence of the different Different Transplanting Periods to survival rate.
After 2~3 true leaves are grown, nursery stock is moved on into even a film greenhouse, described company film greenhouse day temperature
20 DEG C~26 DEG C are set to, nocturnal temperature is set to 10 DEG C~15 DEG C, and humidity is 65~75%, and intensity of illumination is 12000 lx,
Light dark period is set to 12h:12h, so, can just form 4~5 true leaves, and seedling in 60~70 days after seed is sprouted
Survival rate is more than 80%.
The following examples are only intended to describe the present invention in detail, and the scope of the present invention is limited never in any form.
Embodiment 1：In late October, the ripe seed of Carpinus tientaiensis is plucked, suitable elite stand material is selected during seed collecting,
And take into account that forest hip number is more, seed quality preferably feature, the age of tree is more than 50 years, 10~15m of the height of tree, the diameter of a cross-section of a tree trunk 1.3 meters above the ground 15~80
Cm or so, seed is preserved one month in 20 DEG C of ventilations, dry environment.Mature seed 500 is taken, empty grain is being washed away, will be full
Full 405 seeds carry out sprouting experiment, and the seed mass fraction that need to will be sprouted is 10% hypochlorite disinfectant 30min；25
DEG C seed distilled water immersion 48 hours；Immersion treatment 24 hours in the NAA that concentration is 50mg/L is put into again, then is put into concentration be
The GA of 150mg/L3Middle immersion treatment 48 hours；Then it is clean with distilled water flushing, seed is put into germination basin.Germination basin is put into
Illumination box germinates, 25 DEG C of constant temperature of setting, the lx of illumination 1500, and light dark period is 12h: 12h；Radicle is sprouted within 8~11 days.
By radicle sprout Carpinus tientaiensis seed plantation to fill soil culture medium bore for 25cm flowerpot in, soil incubation basigamy
Than being high mountain fallen leaves humus soil: river sand=2: 1；Flowerpot is put into phjytotron, sets 20 DEG C of constant temperature, and humidity is 75%, light
According to 3150 lx, light dark period is 12h:12h, soil culture medium water-holding capacity is controlled 35~50%, and cotyledon is unearthed within 5~7 days,
Rough leaf grows after 15 days, and second true leaf grows after 20 days, the 23rd day seed germination number 213, germination rate 53%；
Treat that 2~3 true leaves grow at the beginning of 4 months, seedling is moved on to an even film greenhouse, day temperature is 20~26 DEG C, and nocturnal temperature is 10
~15 DEG C, humidity is 70%, the lx of illumination 12000, forms 4~5 leaf seedling 178 within 70 days after Seed Treatment, transplants seedling percent
83.6%。
Embodiment 2：In late October, 500 ripe, seed of Carpinus tientaiensis is plucked, empty grain is being washed away, by full 403
Seed carries out sprouting experiment, and its storage procedures and broken dormancy method are with embodiment 1, except that the balcony that radicle is sprouted
Carpinus turczaninowii seed is planted in the cultivation box for filling soil culture medium, and after Seed Treatment, rough leaf grows after 15 days, 21 days
Second true leaf grows afterwards, 23 days seed germination numbers 200, germination rate 49.6%.Treat that 2~3 true leaves grow at the beginning of 4 months, children
Seedling moves on to an even film greenhouse, and day temperature is 20~26 DEG C, and nocturnal temperature is 10~15 DEG C, and humidity is 72%, illumination 12000
Lx, forms 4~5 leaf seedling 160 in 70 days after Seed Treatment, transplants seedling percent 80%.
Embodiment 3：In late October, the ripe seed of Carpinus tientaiensis is plucked, 500, washing away empty grain, will be full
390 seeds carry out sprouting experiment, its storage procedures and broken dormancy method with embodiment 1, except that by radicle sprout
To intelligent glass sunlight house, it is 100cm, the seedling culture of thickness 5cm to make width with soil culture medium to the plantation of Carpinus tientaiensis seed
Bed；Described soil culture medium is high mountain fallen leaves humus soil: river sand=2: 1,20 DEG C of constant temperature is set, and humidity is 75%, illumination
3150 lx, 12h:12h light dark periods, soil culture medium water-holding capacity is controlled 45%.Cotyledon is unearthed within 5~7 days, 14~16 days
Rough leaf grows afterwards, and second true leaf grows within 20~25 days, the 28th day seed germination number 206, and germination rate is
52.84%；Treat that 2~3 true leaves grow, 20~26 DEG C of day temperature of setting, 10~15 DEG C of nocturnal temperature, humidity is 82%, light
According to 12000 lx, 4~5 leaf seedling 173 is formed within 68 days after Seed Treatment, transplant seedling percent 84%.
Embodiment 4：In early November, the ripe seed of Carpinus tientaiensis is plucked, 500, washing away empty grain, will be full
396 seeds carry out sprouting experiment, and its storage procedures and broken dormancy method are with embodiment 1, except that soil culture medium
Composition is high mountain yellow soil, organic fertilizer and river sand, and described proportioning is high mountain yellow soil: organic fertilizer: river sand=2: 1: 1,5
Cotyledon is unearthed within~7 days, and rough leaf grows after 14~15 days, and second true leaf grows within 22~26 days, the 28th day seed germination
Number 161, germination rate is 41%；The Carpinus tientaiensis seed that radicle is sprouted is planted and arrives above-mentioned soil culture medium, increase N,
The content of P, K, carries out nursery, forms within 70 days after treatment 4~5 leaf seedling 81, and its survival rate of seedling is 50%.
Embodiment 5：In late October, the ripe seed of Carpinus tientaiensis is plucked, suitable elite stand material is selected during seed collecting,
And take into account that forest hip number is more, seed quality preferably feature, the age of tree is more than 50 years, 10~15m of the height of tree, the diameter of a cross-section of a tree trunk 1.3 meters above the ground 15~80
Cm or so, seed is preserved 20 days in 10 DEG C of ventilations, in dry environment.Mature seed 50 is taken, empty grain is being washed away, by full 46
Seed carries out sprouting experiment, and the seed mass fraction that need to will be sprouted is 10% hypochlorite disinfectant 30min；25 DEG C of seeds
Distilled water immersion 36 hours；The GA that concentration is 150mg/L is put into again3Middle immersion treatment 20 hours；Then it is dry with distilled water flushing
Only, seed is put into germination basin；Germination basin is put into illumination box germination, sets 20 DEG C of constant temperature, the lx of illumination 1300, brightness week
Phase is 12h: 12h；Radicle is sprouted within 8~11 days.The Carpinus tientaiensis seed plantation that radicle is sprouted is to filling soil culture medium
Bore is in the flowerpot of 25cm, soil culture medium proportioning is high mountain fallen leaves humus soil: river sand=1.5: 0.6；Flowerpot is put into manually
Climatic chamber, sets 20 DEG C of constant temperature, and humidity is 75%, the lx of illumination 3000, and light dark period is 12h:12h, soil culture medium water holding
30%, cotyledon is unearthed within 5~7 days, and rough leaf grows after 15 days, and second true leaf grows after 20 days, the 23rd day for amount control
Seed germination number 20, germination rate 43.4%；Treat that 2~3 true leaves grow, seedling is moved on to even film greenhouse, day temperature
It is 20~26 DEG C, nocturnal temperature is 10~15 DEG C, and humidity is 65%, the lx of illumination 10000, forms 4~5 within 70 days after Seed Treatment
Leaf seedling 16, transplants seedling percent 80%.
Embodiment 6：In early November, the ripe seed of Carpinus tientaiensis is plucked, suitable elite stand material is selected during seed collecting,
And take into account that forest hip number is more, seed quality preferably feature, the age of tree is more than 50 years, 10~15m of the height of tree, the diameter of a cross-section of a tree trunk 1.3 meters above the ground 15~80
Cm or so, seed is preserved 50 days in 25 DEG C of ventilations, in dry environment.Mature seed 50 is taken, empty grain is being washed away, by full 45
Seed carries out sprouting experiment, and the seed mass fraction that need to will be sprouted is 10% hypochlorite disinfectant 30min；30 DEG C of seeds
Distilled water immersion 60 hours；It is immersion treatment 30 hours in the NAA of 100mg/L to be put into concentration again；Then it is dry with distilled water flushing
Only, seed is put into germination basin；Germination basin is put into illumination box germination, sets 30 DEG C of constant temperature, the lx of illumination 1800, brightness week
Phase is 12h: 12h；Radicle is sprouted within 8~11 days.The Carpinus tientaiensis seed plantation that radicle is sprouted is to filling soil culture medium
Bore is in the flowerpot of 25cm, soil culture medium proportioning is high mountain fallen leaves humus soil: river sand=3: 1.2；Flowerpot is put into artificial gas
Room is waited, 22 DEG C of constant temperature is set, humidity is 80%, the lx of illumination 3500, and light dark period is 12h:12h, soil culture medium water-holding capacity
50%, cotyledon is unearthed within 5~7 days, and rough leaf grows after 15 days, and second true leaf grows after 20 days, the 23rd day kind for control
Sub- rudiment number 9, germination rate 20%；Treat that 2~3 true leaves grow, seedling move on to even a film greenhouse, day temperature be 20~
26 DEG C, nocturnal temperature is 10~15 DEG C, and humidity is 75%, the lx of illumination 15000, forms 4~5 leaf seedlings 6 within 70 days after Seed Treatment
, transplant seedling percent 67%.
Embodiment of the present invention is described in detail above in conjunction with embodiment, but the present invention is not limited to above-mentioned reality
Mode is applied, to those skilled in the art, can also under the premise of not departing from the present invention be made some modifications and be changed
Enter, these also should be regarded as belonging to protection scope of the present invention.
Claims (10)
1. a kind of artificial fecundation method of Carpinus tientaiensis, it is characterised in that：Described method is comprised the following steps：
（1）, pluck seed
Carpinus tientaiensis mature seed is plucked, plucking time is annual 10 middle of the month to early November；
（2）, storage
The Carpinus tientaiensis mature seed that will be gathered, is stored in indoor temperature for 10 DEG C~25 DEG C, and ventilation, dry place are protected
The time is deposited for 20 days~150 days；
（3）, the broken dormancy of seed
The seed learnt from else's experience after preserving, washes away empty grain, and seed is put into thimerosal carries out disinfection treatment, then uses distilled water flushing
Totally；Seed is placed in 20 DEG C~30 DEG C distilled water is again soaked 36~60 hours；
The seed after sterilization is placed in plant hormone is again soaked, the concentration of described plant hormone be set to 50mg/L~
200mg/L；
Seed after soaking, it is clean with distilled water flushing, seed is put into germination basin；Described germination basin is put into illumination cultivation
Case germinates, and the temperature setting of described germination basin is 20 DEG C~30 DEG C, and intensity of illumination is 1300 lx~1800 lx, brightness week
Phase is 12h: 12h；
（4）, selection soil culture medium nursery
The Carpinus tientaiensis seed that radicle is sprouted is planted to the cultivation box or flowerpot for filling soil culture medium or intelligent glass sunlight house
In, described soil culture medium proportioning is high mountain fallen leaves humus soil: river sand=1.5~3: 0.6~1.2, the soil incubation
The pH of base is 5.3~5.8, and cultivation box is put into phjytotron, and temperature setting is 18 DEG C~22 DEG C, and humidity is 70~80%, light
It is 3000 lx~3500 lx according to intensity, light dark period is 12h:12h, soil culture medium water-holding capacity is set to 35~
50%, treat that second true leaf grows, phjytotron temperature setting is 20 DEG C~28 DEG C, and humidity is set to 65~75%, illumination
Intensity is set to 7300 lx~8000 lx, and light dark period is set to 12h: 12h；
（5）, Nursery stock transplanting
After 3~5 true leaves are grown, nursery stock is moved on into an even film greenhouse, described company film greenhouse day temperature is set
Be 20 DEG C~26 DEG C, nocturnal temperature is set to 10 DEG C~15 DEG C, humidity is 65~75%, intensity of illumination be 10000 lx~
15000 lx, light dark period is set to 12h: 12h.
2. the artificial fecundation method of a kind of Carpinus tientaiensis according to claim 1, it is characterised in that：Described step
（2）In, storage temperature is 19 DEG C~21 DEG C, and the described holding time is 30~40 days.
3. the artificial fecundation method of a kind of Carpinus tientaiensis according to claim 1, it is characterised in that：Described step
（3）In thimerosal for mass fraction be 10% liquor natrii hypochloritis, disinfecting time is set to 20 min~40min, Ran Houyong
Distilled water flushing is clean.
4. the artificial fecundation method of a kind of Carpinus tientaiensis according to claim 1, it is characterised in that：Described step
（3）In thimerosal be H that mass fraction is 3%2O2Solution, disinfecting time is set to 20 min~30min, then distilled water punching
Wash clean.
5. the artificial fecundation method of a kind of Carpinus tientaiensis according to claim 1, it is characterised in that：Described step
（3）In plant hormone be set to NAA, the concentration of described NAA is set to 50mg/L~200mg/L, described immersion treatment
Set of time is 18~30 hours.
6. the artificial fecundation method of a kind of Carpinus tientaiensis according to claim 1, it is characterised in that：Described step
（3）In plant hormone be set to GA3, described GA3Concentration be set to 50mg/L~200mg/L, described immersion treatment
Set of time is 36~60 hours.
7. the artificial fecundation method of a kind of Carpinus tientaiensis according to claim 1, it is characterised in that：Described step
（3）In plant hormone be set to 6-BA, the concentration of described 6-BA is set to 50mg/L~150mg/L, at described immersion
Reason set of time is 36~60 hours.
8. the artificial fecundation method of a kind of Carpinus tientaiensis according to claim 1, it is characterised in that：Described step
（3）In plant hormone be set to NAA and 6-BA, the concentration of described NAA is set to 50mg/L, during described immersion treatment
Between be 20~28 hours；The concentration of described 6-BA is set to 50mg/L~200mg/L, and described immersion treatment set of time is
45~50 hours.
9. the artificial fecundation method of a kind of Carpinus tientaiensis according to claim 1, it is characterised in that：Described step
（3）In plant hormone be set to NAA and GA3, the concentration of described NAA is set to 50mg/L, described immersion treatment time
It is set to 24 hours；Described GA3Concentration be set to 50mg/L~200mg/L, described immersion treatment set of time is 45
~50 hours.
10. the artificial fecundation method of a kind of Carpinus tientaiensis according to claim 1, it is characterised in that：Described step
（4）In, described soil culture medium proportioning is high mountain fallen leaves humus soil: river sand=2: 1, the pH of the soil culture medium are
5.6, the temperature setting of described phjytotron is 20 DEG C, and humidity is set to 75%, and intensity of illumination is 3100lx~3200lx,
Light dark period is 12h:12h, soil culture medium water-holding capacity is controlled 35~50%, treats that second true leaf grows, artificial climate
Room temperature is set to 25 DEG C, and humidity is set to 70%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510261102.3A CN104956976B (en) | 2015-05-21 | 2015-05-21 | A kind of artificial fecundation method of Carpinus tientaiensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510261102.3A CN104956976B (en) | 2015-05-21 | 2015-05-21 | A kind of artificial fecundation method of Carpinus tientaiensis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104956976A CN104956976A (en) | 2015-10-07 |
| CN104956976B true CN104956976B (en) | 2017-06-16 |
Family
ID=54211217
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510261102.3A Expired - Fee Related CN104956976B (en) | 2015-05-21 | 2015-05-21 | A kind of artificial fecundation method of Carpinus tientaiensis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104956976B (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL1038446C2 (en) * | 2010-12-12 | 2012-06-18 | Treecure B V | METHOD AND DEVICE FOR TREATING TREES. |
| CN102845303B (en) * | 2011-06-29 | 2014-07-16 | 中国科学院上海生命科学研究院 | Method for enlarging population quantity of Carpinus putoensis Cheng |
| CN102845304B (en) * | 2011-06-29 | 2014-04-09 | 中国科学院上海生命科学研究院 | Germination method for Carpinus putoensis Cheng seed |
| CN102612894A (en) * | 2012-04-28 | 2012-08-01 | 南京林业大学 | Method for improving germination percentage of European hornbeam seeds |
| CN103141312A (en) * | 2013-04-03 | 2013-06-12 | 南京林业大学 | Grafting and seedling raising method for European hornbeam |
| CN103733846A (en) * | 2013-12-27 | 2014-04-23 | 湖南省南岳树木园 | Breeding method of precious tree species |
-
2015
- 2015-05-21 CN CN201510261102.3A patent/CN104956976B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN104956976A (en) | 2015-10-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103081791B (en) | Nutrient solution cultivation method of Chinese yews | |
| CN102835243A (en) | Sonneratia apetala seedling cultivation and forestation method | |
| CN106538395A (en) | A kind of method for improving endangered plants Tibet Paeonia ludlowii planting percent and seedling percent | |
| CN105519417A (en) | Method for raising seedlings in plugs by cutting | |
| CN106818443B (en) | Water culture cutting propagation method for red maple branches | |
| CN103053395A (en) | Hole tray substrate for fast cultivation of bonsai semperflorens and use method | |
| CN105766295B (en) | A kind of artificial breeding method of China top cuckoo nursery stock | |
| CN105027741B (en) | The logical seed germination with a smile of poem beautiful jade and the method for nursery | |
| CN105409518B (en) | The introduction and cultivation method of Yunnan-Tibet lily magnolia | |
| CN103039366B (en) | Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants | |
| CN104642000B (en) | Manglietia lucida seed germinating and seedling method | |
| CN106332716A (en) | Camphor tree cuttage method | |
| CN103493678B (en) | Horse chestnut seedling-cultivating method | |
| CN110326527A (en) | A kind of soilless culture method of color green pepper | |
| CN106942063A (en) | A kind of paulownia tissue culture and rapid propagation method of combination outside sprout-cultivating-bottle technology | |
| CN109220261A (en) | A kind of dove tree seedling breeding method | |
| CN104782455B (en) | Matrix and its application for tobacco axillary bud cutting propagation | |
| CN105393739A (en) | Method for culturing mulberry cutting seedling using phenolic plastic flower mud as matrix | |
| CN105746288B (en) | A kind of method for building up of cassie Asia pine cuttage nursery system | |
| CN110393120A (en) | It is a kind of suitable kind in the cherry sapling multiplication breeding method of Guangxi high and cold mountain area | |
| CN109511534A (en) | Berry seed seed propagation method | |
| CN104956976B (en) | A kind of artificial fecundation method of Carpinus tientaiensis | |
| CN103609415B (en) | A kind of matrix of cultivating climbing plant and the method using matrix cottage propagation | |
| CN109744040A (en) | A kind of cultivation of container seedling technology of the salt tolerant grafting rose of Sharon | |
| CN107182355A (en) | A kind of Malus sieversii seed accelerating germination method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170616 Termination date: 20210521 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |