CN105766295B - A kind of artificial breeding method of China top cuckoo nursery stock - Google Patents
A kind of artificial breeding method of China top cuckoo nursery stock Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a kind of artificial breeding methods of China top cuckoo nursery stock, belong to rare plant artificial breeding technology field, include the following steps：Fruit acquisition, Seed storage, seed sprouting, first time hardening, second of hardening and Nursery stock transplanting; utilize the artificial breeding method of the present invention; keep the germination rate of China's top cuckoo seed high; its germination rate can be more than 40%; and seedling percent is high, for total survival rate up to 75%, the seedling growth of incubation is good; be conducive to protect rare or endangered species China top cuckoo, be also beneficial to develop and utilize the plant as ornamental flower kind.
Description
Technical field
The invention belongs to rare plant artificial breeding technology fields, and in particular to a kind of rare plant China top cuckoo nursery stock
Artificial breeding method.
Background technology
China top cuckoo（Rhododendronhuadingense）For the distinctive rhododendron in Zhejiang, China top cuckoo master
It is distributed in Tiantai County in Zhejiang Province Mount Huading, between 750 ~ 950 m of height above sea level, winding also has nature zhejiang pan'an is big
Distribution, distributed area are narrow.The bud of China top cuckoo is born in Zhi Ding, April at florescence, spends big gorgeous, and first leaf opens, and color is various, shape
State is graceful, is a kind of ornamental plant having very much Development volue.
The setting percentage of China top cuckoo is not low, per fruit and seed number at most up to 100；But its own breeding system, which exists, to be lacked
It falls into, such as Some seeds depauperation, germination rate is less than 40%；Seed is without rest period, in the fall after maturation, if condition is suitable
It can preferably sprout, but sprout the seedling formed and be difficult to resist winter boisterous influence, be easy death, survival rate is very low；It is more
Several sons are sprouted in next year 3, April, and because seed is small, contained nutriment is less, and the metabolic consumption of seeds self is big
The nutrient of amount, germination rate is very low, and it is very thin and delicate to sprout the seedling formed, it is difficult to spend hot summer, survival rate is also very low.Cause
This, China's top cuckoo quantity is seldom, there was only 330 plants in balcony Mount Huading at present, belongs to minimum population, it is rare to be put into Zhejiang Province
Endangered plants register is protected.
In big winding two Natural Populations in Tiantai County Mt. Tiantai and Panan County, the seedling of China top cuckoo is rare, breeding
Ability is almost lost；Meanwhile effective method is not yet established at present for the artificial breeding of China top cuckoo.In order to protect rare be on the point of
Danger plant prevents the China's top cuckoo extinction for being in Critical Condition, it is necessary to take safeguard measure and carry out artificial propagation, utilize people
Work mating system, then successful nursery stock transplanting is bred to suitable environment, to expand the quantity of population, this is rare for protecting
Endangered plants are of great significance.
Invention content
It is an object of the invention to overcome under natural conditions China top cuckoo reproduction ability it is weak, manual method also be difficult to breeding at
The technical issues of work(, provides a kind of artificial breeding method of China top cuckoo nursery stock, and the nursery stock survival rate of China's top cuckoo breeding is made to carry
Height protects the rare or endangered species to expand the quantity of China top cuckoo population, and can breed to scale China top cuckoo nursery stock.
In order to solve the above technical problem, the present invention provides a kind of artificial breeding methods of China top cuckoo nursery stock, including with
Lower step.
（1）Fruit acquires：The ripe China's top cuckoo fruit of acquisition, acquisition time is annual early November to late November.
（2）Seed storage：Natural air drying 4 ~ 6 days, by hand stripping capsule taking-up kind at 20~30 DEG C by the fruit adopted
The seed taken is positioned in sealing container by son, is stored under -10 DEG C~-20 DEG C low temperature, and period of storage is 3 ~ 12 months.
（3）Seed is sprouted：Well-developed seed is selected, is cleaned seed disinfection liquid disinfectant, then with distilled water,
Using culture dish double-layer filter paper method, germination test is carried out in illumination box, the Germination Temperature is set as 15 ~ 25 DEG C,
Light application time is 3 ~ 12 hours/day, and intensity of illumination is 1000 ~ 2500 lx.
（4）First time hardening：The 4th day to the 7th day after seed sprouting, by seedling replanting to matrix full of nutrition
In, hardening is carried out in illumination box, light application time is set as 8 ~ 12 hours/day, and intensity of illumination is set as 2000 ~ 5000
Lx, setting temperature are 15 ~ 25 DEG C, and relative humidity is 70 ~ 85%, and watering in 1 to 2 day is primary, make the water-holding capacity of matrix be maintained at 50 ~
85%, it is 1 ~ 3 month to cultivate the time.
（5）Second of hardening：After first time hardening, the seedling replanting with 3 or more true leaves is formed to being arranged
In the flowerpot of cultivation matrix, 3 ~ 6 plants are planted per basin, in the controlled environment chamber in hardening again；The cultivation matrix quality proportioning
For mountainous region humus soil: fine sand: base manure=4~6: 2~4: 1~3, the light application time of the phjytotron is set as 8 ~ 12
Hour/day, setting intensity of illumination are 5000 ~ 10000 lx, and temperature is 15 ~ 25 DEG C, and relative humidity is 60 ~ 85%；It waters within 2 to 3 days
Once, the water-holding capacity of matrix is made to be maintained at 30 ~ 60%, it is 2 ~ 6 months to cultivate the time.
（6）Nursery stock transplanting：After second of hardening, the nursery stock of high 30 ~ 50cm is formed, is transplanted from phjytotron
To corresponding natural environment or carry out potting.
As a preferred solution of the present invention, in the step（1）In, the acquisition time is annual November
The middle ten days to late November.
As a preferred solution of the present invention, in the step（2）In, the storage temperature is set as -10 DEG C
~-20 DEG C, period of storage is 3 ~ 6 months.
As a preferred solution of the present invention, in the step（3）In, the thimerosal is 10% sodium hypochlorite,
The Germination Temperature is 18 ~ 22 DEG C, and light application time is 8 ~ 10 hours/day, and intensity of illumination is 1500 ~ 1800 lx.
As a preferred solution of the present invention, in the step（4）In, it is described in the illumination box
Light application time be 8 ~ 10 hours/day, intensity of illumination be 3000 ~ 4000 lx, temperature be 20 ~ 22 DEG C, relative humidity be 75 ~
80%, the water-holding capacity of matrix is maintained at 70 ~ 80%, and it is 2 ~ 3 months to cultivate the time, to enhance adaptability of the seedling to environment.
As a preferred solution of the present invention, in the step（5）In, the light application time of the phjytotron
For 8 ~ 10 hours/day, intensity of illumination is 7000 ~ 8000 lx, and temperature is 20 ~ 22 DEG C, and relative humidity is 70 ~ 80%；Matrix is held
Water quantity holding is 40 ~ 50%, and it is 4 ~ 5 months to cultivate the time, and nursery stock vitality at this time is most prosperous, is easy to transplant survival.
The beneficial effects of the present invention are：（1）The germination rate of seed is high, wherein the seed of storage 3 ~ 6 months, germination rate
Can be more than 40%.
（2）Seedling percent is high, the seedling normally sprouted, is less than 10% in the death rate in hardening stage first time, second
The death rate in secondary hardening stage is less than 20%.
（3）The seedling growth of incubation is good, and beautiful design, is suitable for garden planting and potting, to form scale
China top cuckoo seedling-wood breeding base.
（4）The present invention is conducive to push up rare or endangered species China the protection of cuckoo, is also beneficial to using the plant as ornamental
Flower variety is developed and utilized.
Description of the drawings
Fig. 1 is a kind of artificial breeding method's flow chart of China top cuckoo nursery stock of the present invention.
Specific implementation mode
The present invention will be further described below with reference to the drawings.
A kind of artificial breeding method of China top cuckoo nursery stock as shown in Figure 1, includes the following steps：（1）Fruit acquires：It adopts
The ripe China's top cuckoo fruit of collection, acquisition time is annual early November to late November；China's top cuckoo ripening fruits should be in due course
Acquisition, the fruit of different time acquisition, the development condition of seed is different, and germination rate also has very big difference；It is sent out by studying
Existing, under identical mating system, the fruit that annual mid-November to late November is acquired, the germination rate of seed is relatively
It is high.
（2）Seed storage：Using different store methods, the germination rate of China's top cuckoo seed has very big difference；Through research, fit
Suitable store method be by fruit natural air drying, then peel off capsule take out seed, stored at low temperature with sealing container；Such as it will
The fruit adopted natural air drying 4 ~ 6 days at 20~30 DEG C peel off capsule and take out seed, the seed taken are positioned over close by hand
It seals in container, is stored under -10 DEG C~-20 DEG C low temperature, period of storage is 3 ~ 12 months；Since the seed very little of cuckoo is pushed up by China,
Contained nutriment is less, and the metabolism of seeds self can consume a large amount of nutrient, so that seed sprouting is affected, therefore need
Cord blood is carried out, by the way that the study found that under identical mating system, setting storage temperature is -10 DEG C~-20 DEG C, storage
Time is 3 ~ 6 months, and the germination rate of seed is relatively high.Above-mentioned sealing container can use plastic seal pocket double casing, effect
Fruit is preferable.
（3）Seed is sprouted：Well-developed seed is selected, the seed that hundred air dry weights are more than 0.12g can be generally selected,
Its intraseminal nutriment is more, sprouts preferably, and then seed is used and sterilizes liquid disinfectant, then is cleaned with distilled water；Through
Research, it is identical in other sprouting conditions, when no light, light application time deficiency or intensity of illumination are less than 500lx,
The germination rate of seed is very low（Referring to table 1）.The present invention uses culture dish double-layer filter paper method, is sprouted in illumination box
Experiment, the Germination Temperature are 15 ~ 25 DEG C, and light application time is 3 ~ 12 hours/day, and intensity of illumination is 1000 ~ 2500 lx；Make
For the preferred embodiment of the present invention, the thimerosal is 10% sodium hypochlorite, and the Germination Temperature is 18 ~ 22 DEG C, illumination
Time is 8 ~ 10 hours/day, and intensity of illumination is set as 1500 ~ 1800 lx；Using the above method, opened by 10 days or so seeds
Begin to sprout.
The influence of cuckoo seed sprouting is pushed up in 1 illumination of table to China
（4）First time hardening：After seed is sprouted, seedling needs to be transplanted to hardening in matrix full of nutrition in due course；Generally
The 4th day to the 7th day after seed sprouting, by seedling replanting to matrix full of nutrition, since matrix contains abundant battalion
Substance is supported, disclosure satisfy that the growth needs of China top cuckoo seedling.Through research, if other conditions are the same, cultivation matrix
The survival rate of seedling is influenced very big；Such as matrix is done with paddy field soil and organic fertilizer mixing, seedling development is slow, and dead
Rate is high, and the survival rate of seedling is less than 20%；Seedling matrix is done with high mountain yellow soil and organic fertilizer mixing, survival rate after cultivating 1 month
Less than 50%；It is mixed with nutrient matrix and fine sand, survival rate is more than 90%（Referring to table 2）.Then it is refined in illumination box
Seedling, setting light application time are 8 ~ 12 hours/day, and intensity of illumination is 2500 ~ 5000 lx, and temperature is 15 ~ 25 DEG C, and relative humidity is
70 ~ 85%, watering in 1 to 2 day is primary, and the water-holding capacity of matrix is made to be maintained at 50 ~ 85%, and it is 1 ~ 3 month to cultivate the time；As the present invention
A preferred embodiment, in illumination box, setting light application time be 8 ~ 10 hours/day, intensity of illumination be 3000 ~ 4000
Lx, temperature are 20 ~ 22 DEG C, and relative humidity is 75 ~ 80%, and the water-holding capacity of matrix is maintained at 70 ~ 80%, and it is 2 ~ 3 months to cultivate the time；
By hardening, adaptability of the seedling to environment can be enhanced.
The different cultivation matrixes of table 2 push up the influence of cuckoo seedling percent to China
（5）Second of hardening：Seedling through first time hardening needs to be transplanted in due course in certain cultivation matrix refines again
Seedling.It has been investigated that different times transplant and different cultivation matrixes, seedling percent are used to have very big difference (referring to table 3).
After first time hardening, being formed in the seedling replanting with 3 or more true leaves to the flowerpot of setting cultivation matrix, per basin kind
Plant 3 ~ 6 plants, in the controlled environment chamber in hardening again；The cultivation matrix quality proportioning is mountainous region humus soil: fine sand: base manure
=4~6: 2~4: 1~3, in second of hardening, need to properly increase intensity of illumination and light application time, the artificial climate
The light application time of room be 8 ~ 12 hours/day, intensity of illumination be 5000 ~ 10000 lx, temperature be 15 ~ 25 DEG C, relative humidity be 60 ~
85%；Watering in 2 to 3 days is primary, and the water-holding capacity of matrix is made to be maintained at 30 ~ 60%, and it is 2 ~ 6 months to cultivate the time；As the present invention's
One preferred embodiment, light application time in the controlled environment chamber are set as 10 ~ 11 hours/day, and intensity of illumination is set as 7000 ~ 8000
Lx, temperature setting are 20 ~ 22 DEG C, and relative humidity is set as 70 ~ 80%；The water-holding capacity of matrix is maintained at 40 ~ 50%, cultivates the time and is
4 ~ 5 months；Survival rate by second of hardening, the well-grown of nursery stock, transplanting is high.
The influence of table 3 different transplanting times and cultivation matrix to seedling percent
（6）Nursery stock transplanting：After second of hardening, the nursery stock of high 30 ~ 50 cm is formed, is moved from phjytotron
It plants corresponding natural environment or carries out potting.
Embodiment 1：In mid-November, China's top cuckoo fruit is picked, is air-dried 5 days in the environment of the aeration-drying at 25 DEG C, stripping
Open capsule, take full seed 200, carry out sprouting experiment after being preserved 90 days at -20 DEG C, seed with 10% hypochlorite disinfectant
10min, 20 DEG C of distilled water immersions 8 hours；Seed is put into germination basin again, germinates in illumination box, 20 DEG C of temperature, light is set
It is 1500 lx, 8 hour/day of light application time according to intensity；Start to sprout within 10th day, the 17th day total sprouting number 95, germination rate is
48%.In the flowerpot for being 25cm by the seedling culture transferring of sprouting to bore, nutrient matrix in flowerpot: fine sand mass ratio is 3: 1, in light
According to first time hardening is carried out in incubator, 20 DEG C of constant temperature is set, humidity 75%, 2500 lx of illumination, light application time 12 hours/
It, matrix water-holding capacity is controlled 75%, is grown by 10 ~ 15 days rough leafs, is grown by 20 ~ 25 days second true leaves,
It is grown by 28 ~ 40 days third piece true leaves, the 4th true leaf occurs within the 45th day；There to be 4 ~ 5 true leaf seedling, 86 plants of shiftings again
It plants and carries out second of hardening in cultivation matrix, which is mountainous region humus soil: fine sand: base manure 5: 2: 1,
Hardening again in the controlled environment chamber, setting light application time are 10 hours/day, and day temperature is 20 ~ 25 DEG C, nocturnal temperature is 15 ~
20 DEG C, relative humidity 75%, intensity of illumination is 8000 lx, the water-holding capacity of matrix is 60%, forms within the 60th day high >=35 cm
72 plants of nursery stock, by the above method, first time hardening seedling percent is 90.5%, second of hardening seedling percent 83.7%；
Again from phjytotron in seedling replanting to corresponding natural environment.
Embodiment 2：In late November, China's top cuckoo fruit is picked, is air-dried 6 days in the environment of the aeration-drying at 20 DEG C, stripping
Capsule is opened, full seed 200 is taken, carries out sprouting experiment after being preserved 120 days at -10 DEG C, seed is disappeared with 10% sodium hypochlorite
Poison 10 min, 20 DEG C of distilled water immersions 10 hours；Seed is put into germination basin again, germinates in illumination box, temperature 15 is set
DEG C, intensity of illumination is 1000 lx, 5 hour/day of light application time；Start to sprout within 10th day, total sprouting number of the 17th day seed is
87, germination rate 43.6%.In the flowerpot for being 25cm by the seedling culture transferring of sprouting to bore, nutrient matrix in flowerpot: fine sand quality
Than being 2: 1, first time hardening is carried out in illumination box, setting temperature is 20 DEG C, humidity 80%, 3000 lx of illumination, light
According to 10 hour/day of time, matrix water-holding capacity control grown in 80%, the 12nd ~ 16 day rough leaf, the 20th ~ 25 day second
True leaf is grown, and third piece true leaf is grown within the 30th ~ 42 day, starts within the 46th day to grow the 4th true leaf, seedling numbers are 79 plants；Again will
4 ~ 5 79 plants of true leaf seedling are transplanted in cultivation matrix and carry out second of hardening, which is mountainous region humus soil: fine sand
: the mass ratio of base manure is 5: 3: 2, and hardening, setting light application time are 11 hours/day again in the controlled environment chamber, and day temperature is
22 DEG C, nocturnal temperature is 15 DEG C, relative humidity 80%, and intensity of illumination is 9000 lx, and the water-holding capacity of matrix is 50%, the 80th day
Formed it is high >=35 cm 62 plants of nursery stock, by the above method, first time hardening seedling percent is 90.8%, second of hardening children
Shoot survival percent 78.5%, then from phjytotron in seedling replanting to corresponding natural environment.
Embodiment 3：In mid-November, China's top cuckoo fruit is picked, is air-dried 5 days in the environment of the aeration-drying at 25 DEG C, stripping
Capsule is opened, full seed 200 is taken, carries out sprouting experiment after being preserved 180 days at -20 DEG C, seed is disappeared with 10% sodium hypochlorite
Poison 10 min, 20 DEG C of distilled water immersions 8 hours；Seed is put into germination basin again, germinates in illumination box, temperature 25 is set
DEG C, intensity of illumination 2000lx, light application time is 3 hours/day；Start to sprout within 11st day, total sprouting number of the 17th day seed is
70, germination rate 35%；In the flowerpot for being 25cm by the seedling culture transferring of sprouting to bore, nutrient matrix in flowerpot: fine sand mass ratio
It is 3: 1, first time hardening is carried out in illumination box, setting temperature is 15 DEG C, humidity 70%, intensity of illumination 2000lx,
8 hour/day of light application time, the control of matrix water-holding capacity were grown in 50%, the 11st ~ 16 day rough leaf, the 18th ~ 25 day second
Piece true leaf is grown, and third piece true leaf is grown within the 28th ~ 40 day, starts within the 45th day to grow the 4th true leaf, what the 4th true leaf was grown
Seedling numbers are 56 plants；4 ~ 5 56 plants of true leaf seedling are transplanted in cultivation matrix again and carry out second of hardening, the cultivation matrix
For mountainous region humus soil: fine sand: base manure mass ratio is 4: 2: 1, hardening, setting light application time are 8 small again in the controlled environment chamber
When/day, day temperature is 25 DEG C, and nocturnal temperature is 20 DEG C, and relative humidity 60%, intensity of illumination is 6000 lx, and matrix is held
Water is 30%, formed within the 80th day it is high >=35 cm 37 plants of nursery stock, by the above method, first time hardening seedling percent is
80%, second of hardening seedling percent 66.0%, then from phjytotron in seedling replanting to corresponding natural environment.
Embodiment 4：In late November, China's top cuckoo fruit is picked, is air-dried 6 days in the environment of the aeration-drying at 20 DEG C, stripping
Capsule is opened, full seed 200 is taken, carries out sprouting experiment after being preserved 360 days at -20 DEG C, seed is disappeared with 10% sodium hypochlorite
Malicious 10min, 20 DEG C of distilled water immersions 10 hours；Seed is put into germination basin again, germinates in illumination box, temperature 25 is set
DEG C, intensity of illumination is 2500 lx, 12 hour/day of light application time；Start to sprout within 9th day, the 17th day total sprouting number is 63, is sprouted
Rate 31.5%.In the flowerpot for being 25cm by the seedling culture transferring of sprouting to bore, nutrient matrix in flowerpot: fine sand mass ratio is 2: 1,
The progress first time hardening in illumination box, 25 DEG C of constant temperature of setting, humidity 85%, 5000 lx of illumination, light application time 12 are small
When/day, and the control of matrix water-holding capacity grows in 85%, the 11st ~ 15 day rough leaf, and second true leaf is grown within the 20th ~ 25 day, and the
Third piece true leaf is grown within 28 ~ 40 days, the 4th true leaf is occurred within the 45th day, is amounted to 45 plants of seedling；By the seedling 45 of 4 ~ 5 true leaves of band
Strain, which is transplanted in cultivation matrix, carries out second of hardening, which is mountainous region humus soil: fine sand: base manure mass ratio is 6:
4: 3, hardening, setting light application time are 12 hours/day again in the controlled environment chamber, and day temperature is 20 ~ 25 DEG C, and nocturnal temperature is
15 ~ 20 DEG C, relative humidity 85%, intensity of illumination is 10000 lx, and the water-holding capacity of matrix is 60%, forms height >=35 within the 70th day
28 plants of the nursery stock of cm, by the above method, first time hardening seedling percent is 71.4%, and second of hardening seedling percent is
62.2%；Again from phjytotron in seedling replanting to corresponding natural environment.
Embodiment of the present invention is explained in detail above in conjunction with attached drawing, but the present invention is not limited to above-mentioned embodiment party
Formula can also make several variations and modifications under the premise of not departing from the present invention to those skilled in the art, this
It also should be regarded as belonging to the scope of protection of the present invention a bit.
Claims (6)
1. a kind of artificial breeding method of China top cuckoo nursery stock, includes the following steps：
（1）Fruit acquires：The ripe China's top cuckoo fruit of acquisition, acquisition time is annual early November to late November；
（2）Seed storage：By the fruit adopted, natural air drying 4 ~ 6 days, the manual capsule that peels off take out seed at 20~30 DEG C, will
The seed taken is positioned in sealing container, is stored under -10 DEG C~-20 DEG C low temperature, and period of storage is 3 ~ 12 months；
（3）Seed is sprouted：Well-developed seed is selected, is cleaned, is used seed disinfection liquid disinfectant, then with distilled water
Culture dish double-layer filter paper method carries out germination test in illumination box, and the Germination Temperature is set as 15 ~ 25 DEG C, illumination
Time is 3 ~ 12 hours/day, and intensity of illumination is set as 1000 ~ 2500 lx；
（4）First time hardening：The 4th day to the 7th day after seed sprouting, by seedling replanting to matrix full of nutrition,
Hardening is carried out in illumination box, light application time is set as 8 ~ 12 hours/day, and intensity of illumination is set as 2500 ~ 5000lx, setting
Temperature is 15 ~ 25 DEG C, and relative humidity is 70 ~ 85%, and watering in 1 to 2 day is primary, and the water-holding capacity of matrix is made to be maintained at 50 ~ 85%, cultivates
Time is 1 ~ 3 month；
（5）Second of hardening：It is cultivated to setting the seedling replanting with 3 or more true leaves after first time hardening, is formed
In the flowerpot of matrix, 3 ~ 6 plants are planted per basin, in the controlled environment chamber in hardening again；Cultivation matrix quality proportioning used is mountain
Ground humus soil: fine sand: base manure=4~6: 2~4: 1~3, the light application time of the phjytotron be set as 8 ~ 12 hours/
It, setting intensity of illumination is 5000 ~ 10000lx, and temperature is 15 ~ 25 DEG C, and relative humidity is 60 ~ 85%；Watering in 2 to 3 days is primary,
The water-holding capacity of matrix is set to be maintained at 30 ~ 60%, it is 2 ~ 6 months to cultivate the time；
（6）Nursery stock transplanting：After second of hardening, the nursery stock of high 30 ~ 50cm is formed, phase is transplanted to from phjytotron
The natural environment answered or progress potting.
2. a kind of artificial breeding method of China top cuckoo nursery stock according to claim 1, it is characterised in that：In the step
Suddenly（1）In, the acquisition time is annual mid-November to late November.
3. a kind of artificial breeding method of China top cuckoo nursery stock according to claim 1, it is characterised in that：In the step
Suddenly（2）In, the storage temperature is set as -10 DEG C~-20 DEG C, and period of storage is 3 ~ 6 months.
4. a kind of artificial breeding method of China top cuckoo nursery stock according to claim 1, it is characterised in that：In the step
Suddenly（3）In, the thimerosal be 10% sodium hypochlorite, the Germination Temperature be 18 ~ 22 DEG C, light application time be 8 ~ 10 hours/
It, intensity of illumination is 1500 ~ 1800 lx.
5. a kind of artificial breeding method of China top cuckoo nursery stock according to claim 1, it is characterised in that：In the step
Suddenly（4）In, in the illumination box, the light application time is set as 8 ~ 10 hours/day, and intensity of illumination is 3000 ~
4000 lx, temperature are 20 ~ 22 DEG C, and relative humidity is 75 ~ 80%, and the water-holding capacity of matrix is maintained at 70 ~ 80%, and it is 2 ~ 3 to cultivate the time
A month.
6. a kind of artificial breeding method of China top cuckoo nursery stock according to claim 1, it is characterised in that：In the step
Suddenly（5）In, the light application time of the phjytotron is 8 ~ 10 hours/day, and intensity of illumination is 7000 ~ 8000lx, and temperature is
20 ~ 22 DEG C, relative humidity is 70 ~ 80%, and the water-holding capacity of matrix is maintained at 40 ~ 50%, and it is 3 ~ 5 months to cultivate the time.
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| CN108513757A (en) * | 2018-04-03 | 2018-09-11 | 安徽农业大学 | Promote the method for cuckoo germination |
| CN110558130B (en) * | 2019-09-23 | 2021-06-15 | 台州学院 | Cutting method of cauliflower |
| CN112889393A (en) * | 2021-01-21 | 2021-06-04 | 洛阳师范学院 | Method for artificially breeding seedlings of rare or endangered plant lingbao rhododendron seeds |
| CN114586596B (en) * | 2022-03-15 | 2023-08-01 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | Breeding method of special ornamental plant Libo rhododendron seed in China |
| CN115088576B (en) * | 2022-08-22 | 2022-11-08 | 中国科学院昆明植物研究所 | Low-temperature germination and seedling hardening method for alpine flowers and plants of Artemisia |
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| USPP4905P (en) * | 1980-06-25 | 1982-10-26 | Conard-Pyle Company | Rhododendron plant--Hacput variety |
| KR100186675B1 (en) * | 1996-08-26 | 1999-03-20 | 황진선 | Cuttage for hwangcholjook |
| CN1568670A (en) * | 2004-04-26 | 2005-01-26 | 上海市闵行区农业科学研究所 | Tissue culture and tachytelic propagating method for alpine rhododendron |
| CN100444722C (en) * | 2006-01-18 | 2008-12-24 | 贵州省农业科学院生物技术研究所 | Cyrtopterin tissue culturing method and fast reproduction thereof |
| CN100459852C (en) * | 2006-07-21 | 2009-02-11 | 吕平 | A plant quick propagation method |
| KR101557096B1 (en) * | 2008-05-23 | 2015-10-20 | 서희동 | A method to produce tea beverages production water from deep-ocean water and a method to use this for tea beverages production |
| CN102090337A (en) * | 2010-12-13 | 2011-06-15 | 江苏省农业科学院 | Rapid propagation method of rhododendron latoucheae |
| CN102119660B (en) * | 2010-12-16 | 2012-09-05 | 宜昌市科力生实业有限公司 | Method for rooting culture,seedling adaptation and transplantation of alpine rose tissue-cultured seedling in greenhouse |
| CN102498899B (en) * | 2011-11-15 | 2013-03-27 | 云南云科花卉有限公司 | Method for cuttage propagation of rhododendron lapponicum |
| CN103583211B (en) * | 2013-11-13 | 2015-11-04 | 石家庄市神州花卉研究所 | The symbiosis Wicresoft inarching method of helping each other of the stake of wild alpine rhododendron ancient tree and the seedling that grows directly from seeds |
| CN103814736A (en) * | 2014-03-07 | 2014-05-28 | 安徽理工大学 | Cuckoo greenhouse seedling thinning transplantation method |
| CN105191766B (en) * | 2015-09-02 | 2017-09-05 | 云南省农业科学院花卉研究所 | A kind of quick hardening off method of alpine rose tissue-cultured seedling |
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2016
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