CN104956860A - Pseudo-ginseng humidity control dry storage method - Google Patents
Pseudo-ginseng humidity control dry storage method Download PDFInfo
- Publication number
- CN104956860A CN104956860A CN201510363032.2A CN201510363032A CN104956860A CN 104956860 A CN104956860 A CN 104956860A CN 201510363032 A CN201510363032 A CN 201510363032A CN 104956860 A CN104956860 A CN 104956860A
- Authority
- CN
- China
- Prior art keywords
- seed
- seeds
- time
- ginseng
- pseudo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a pseudo-ginseng humidity control dry storage method. The pseudo-ginseng humidity control dry storage method comprises the following steps that 1, seed treatment is conducted, mature fruits of pseudo-ginseng are collected and scrubbed in water to remove fruit peels, mature and plump seeds are obtained, and the seeds are washed with clean water after being sterilized; 2, seed drying is conducted, namely, the seeds obtained in the step 1 are dried in the shade; 3, the seeds processed by the step 2 are mixed with dry perlite, the mixture is filled into open-end containers respectively, then the open-end containers are placed into a closed container for humidity control cold storage, the bottom of the closed container is filled with water, and a baffle with a gap is used for enabling the open-end containers to make no contact with the water; 4, the seeds processed by the step 3 are mixed with wet perlite, and the mixture is subjected to cold storage. By means of the pseudo-ginseng humidity control dry storage method, the seeds are subjected to the humidity control cold storage in a certain environment humidity, and therefore the morphological development time of embryos can be prolonged; the time from embryo sprouting of the seeds to dormancy breaking of the seeds is prolonged, the transport and the storage are facilitated, and the germination rate and the emergence rate after the seeds are planted are facilitated.
Description
Technical field
The present invention relates to a kind of Panax notoginseng seeds control to wet dry method of hiding, belong to traditional Chinese medicine field of storage.
Background technology
Pseudo-ginseng is Araliaceae Panax herbaceos perennial, have another name called invaluable, blood ginseng, pseudo-ginseng etc., its root, stem, leaf, Hua Junke are used as medicine, it is the traditional rare traditional Chinese medicine of China, warm in nature, sweet and slightly bitter taste, has effect of hemostasia and dissipation blood stasis, swelling and pain relieving, is the primary raw material of the Chinese patent drug such as " Yunnan Baiyao ", " Fufang Danshen Pian ", " compound danshen dripping pills ".Modern pharmacological research shows, pseudo-ginseng contains 51 kinds of arasaponins, 77 kinds take beta-elemene as the volatile oil of representative, 17 kinds take dencichine as amino acid and the various active material such as notoginseng polysaccharide, pseudo-ginseng flavones of representative, hematological system, cardiovascular system, nervous system, immune system, metabolic system and anti-inflammatory, anti-ageing, antitumor etc. in all there is pharmacologically active and unique curative effect.Pseudo-ginseng medicinal function is extensive, and pharmacology, drug effect are remarkable, and bioavilability is high, has huge potentiality to be exploited and wide market prospects in fields such as medicine, health products, cosmetics, functional food.In recent years, along with market demand significantly increase and artificially excessively excavate, wild pseudo-ginseng is endangered, at present mainly artificial cultivation.
Very easily inactivation after the seed dehydration of Desiccation Sensitivity, thus lose sprouting ability, pole is storage tolerance not.Research shows, pseudo-ginseng belongs to Desiccation Sensitivity seed, and during physical maturity, the water content of Panax notoginseng seeds is about 70%, and at room temperature (20 DEG C, HR=30%) dry in the shade 1 day, and water content will drop to about 40%, and germination rate drops to about 50%; Dry in the shade 2 days, water content drops to about 20%, and germination rate is only 10%.According to the literature under field conditions (factors) (winter of warm and moist), the life-span of the Panax notoginseng seeds in pseudo-ginseng producing region is only about 15 days.In production, Panax notoginseng seeds with adopting with broadcasting, can not can only be store for a long time, more can not carry out long-distance transportation, seriously hinders the operation of Panax notoginseng seeds and the development of introducing and planting.In addition, pseudo-ginseng producing region also met with the natural calamities such as snow disaster often in recent years, owing to lacking suitable seed storage method, cannot reseed in time on producing, cause huge economic loss, therefore, in cultivation of pseudo-ginseng and production process, Seed storage becomes the limiting factor become increasingly conspicuous.
Panax notoginseng seeds also has morphological dormacy, and the mature seed of newly gathering has spheroidal embryo, not yet grows completely, at normal temperatures husky Tibetan, and after 45 ~ 60 days embryo completion morphologies are grown, seed just starts to sprout.If because the seed of newly gathering effectively processes not in time or sows immediately, seed vitality is just very easily lost, so only hide pseudo-ginseng method with husky under normal temperature in production practices, make seed vitality extend 60 ~ 90 days, but the method can not stop embryo morphological development process.Therefore, cultivation of pseudo-ginseng is badly in need of a kind of method extending the Seed storage time in producing, and to maintain seed vitality for a long time, ensures percentage of seedgermination, improves pseudo-ginseng emergence rate, simultaneously for reseeding when other regional introducing and planting and natural calamity provides condition.
Summary of the invention
The object of this invention is to provide a kind of Panax notoginseng seeds control to wet dry method of hiding, the inventive method can extend the morphological development time of embryo; And extend seed to time of breaking dormancy after embryo germination, with significant prolongation seed storage time and break the dormancy of seed, be convenient to transport and store, be conducive to the germination rate after improving seed plantation and emergence rate.
Panax notoginseng seeds control provided by the invention is wet and is done the method for Tibetan, comprises the steps: 1) seed treatment: the ripening fruits gathering pseudo-ginseng, rub with the hands in water and remove pericarp, obtain the seed of mature and plump, clean with clear water after sterilization;
2) seed drying: by step 1) the described seed that obtains dry in the shade to water content be 50 ~ 58%;
3) by step 2) seed that processes mixes with dry perlite, be divided in open-top receptacle, and then described open-top receptacle is placed in closed container controls clammy Tibetan, add water bottom wherein said closed container, make described open-top receptacle not contact described water with band dummy receptacle;
4) by step 3) seed that processes mixes with moistening perlite, then stored refrigerated.
Ripening fruits described in the present invention is healthy mature fruit.
Above-mentioned method, step 1) in, the reagent of described sterilization is liquor natrii hypochloritis;
The reagent concentration of described sterilization can be 0.1% ~ 2.5%, specifically can be 0.1%;
The time of described sterilization can be 5 ~ 10min, specifically can be 10min;
The object of described sterilization is killed by the microorganism of described the surface of the seed, prevents described seed from going mouldy in follow-up storage; Clean with clear water again after carrying out described sterilization, in order to avoid the reagent of described sterilization remains on described seed, damage is caused to described seed vitality.
Above-mentioned method, the water content after described seed dries in the shade is 50 ~ 58%, specifically can be 55%.
In the present invention, described in dry in the shade and refer to be placed on and avoid direct sunlight, shady and cool place;
Described condition of drying in the shade is: temperature can be 10 ~ 30 DEG C, and specifically can be 20 DEG C, relative moisture can be 20 ~ 70%, specifically can be 40%;
The time of described drying can be 24 ~ 72h, specifically can be 24h.
Above-mentioned method, step 3) in, the perlitic volume ratio of described seed and described drying can be 1:0.5 ~ 2, specifically can be 1:1;
The temperature of the clammy Tibetan of described control can be 2 ~ 4 DEG C, specifically can be 4 DEG C, the time of the clammy Tibetan of described control can be 180 ~ 270d, specifically can be 180d, 240d, 270d or 180 ~ 270d, the relative moisture in described closed container can be 98 ~ 100%, specifically can be 100%, seed is placed in open-top receptacle, being placed on bottom is again equipped with in the closed container of water, makes seed keep original moisture, maintains seed vitality.
Above-mentioned method, step 3) also comprise described open-top receptacle sterilizing and dry and/or carry out breathing freely at least 1 time and the step stirred at the described control During Cold Storage that wets, open-top receptacle described in the present invention can adopt tissue culture bottle, glass open-top receptacle or plastics open-top receptacle.
Above-mentioned method, step 3) in, described breathe freely and stir carry out number of times and can be 1 ~ 9 time, specifically can be 8 times, 9 times or 8 ~ 9 times, every a period of time ventilative and reaction, make seed obtain enough oxygen, breathe and vigor to maintain seed;
Carry out described to breathe freely and the time interval stirred can be 14 ~ 30d, specifically can be 30d.
Above-mentioned method, seed and the described moistening perlitic volume ratio of described refrigeration can be 1:3 ~ 10, specifically can be 1:10.
Above-mentioned method, described moistening perlitic water content can be 20 ~ 80%, specifically can be 80%, and moisture content refers to and calculates according to mass percentage.
Above-mentioned method, step 4) in, the temperature of described refrigeration can be 2 ~ 4 DEG C, and the time of described refrigeration can be 60 ~ 90d;
Step 4) be also included in described During Cold Storage and carry out breathing freely at least 1 time and the step stirred.
Above-mentioned method, step 4) in, described breathe freely and stir carry out number of times and can be 3 ~ 12 times, specifically can be 8 times, 12 times or 8 ~ 12 times;
Carry out described to breathe freely and the time interval stirred can be 7 ~ 30d, breathe freely every a period of time and stir, making seed obtain enough oxygen, with the vigor of sustaining life.
In the present invention, in step 3) storage in seed be in the wet cold storage environment of control, maintain the stage of morphological dormacy, namely embryo there is no development growth or slowly grows, and extends the development time of embryo; In step 4) in seed start to absorb moisture, embryo starts to absorb moisture growth, and seed is breach after refrigeration, after breach 10 days substantially just can rudiment.In step 3) storage stage seed completely meet produce in reseed, the requirement of transport.
The present invention has the following advantages:
1, in the present invention with the airtight container that can control certain environment humidity to the clammy Tibetan of seed control, the embryo development time of seed can be extended, and keep seed to sustain life required moisture, be beneficial to the preservation of seed and the transport of seed.
2, the holding time of seed is extended: the storage time of method energy significant prolongation seed of the present invention, make storage time by about 15 under natural conditions day, extend at most 360 days, pseudo-ginseng can be improved to survive the winter cultivation mode of emerging, be convenient to large-scale introducing and planting, reseed in time when can meet with natural calamity in pseudo-ginseng producing region provides high quality seed simultaneously.
3, moistening perlite can meet the water demand of seed embryonic development preferably, and timing is stirred ventilative, makes seed keep vitality.
4, the seed of the present invention's storage, the breaking dormancy phase, the germination rate of seed is high, and it is high to plant later emergence rate.
4, simple: equipment required for the present invention is simple, and whole process concisely facilitates, and processing cost is low, is convenient to promote the use of aborning.
Embodiment
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
The method of the storage of embodiment 1, Panax notoginseng seeds
1) acquisition of seed: the healthy plant gathering life in 3 years, when fruit maturation, gather in batches, connect bennet together to take, removing floral disc and immature fruit, ripening fruits is washed by rubbing with the hands in clear water, remove exocarp and obtain seed, after cleaning with clear water, then use clorox (NaClO) aqueous solution sterilization 10min of 0.1%, more repeatedly clean with clear water.
2) seed drying: put 20 DEG C, relative moisture is the 24h that dries in the shade under 40% condition, obtain the seed that water content is 55%.
3) the wet storage of low temperature control: by step 2) perlite of the seed that processes and drying is that 1:1 mixes with volume ratio, loaded in tissue culture bottle (being open-top receptacle), again tissue culture bottle is put into the closed container that water is equipped with in bottom, make open-top receptacle not contact the water of bottom with band dummy receptacle bottom closed container, in closed container, relative moisture is 100%.Then fill above-mentioned the wet stored refrigerated of refrigerator control that seed-bearing closed container puts into 4 DEG C, the cryopreserved time is 240 days, and before 240 days storages, constantly detect the germination rate of seed, result is as shown in table 1; Breathed freely every 1 month during stored refrigerated and stir seed, carrying out 8 times altogether and breathe freely and stir seed, to maintain seed appropriate aqueous amount and enough oxygen, sustain life power.
4) take out in step 3) the wet storage seed of 240 days of middle control, using water content to be the perlite of 80%, is that 10:1 mixes according to perlite and seed volume ratio, storing, storing 60 days with being positioned over 4 DEG C of refrigerators after the packing of bundle aperture valve bag; Breathe freely week about between the storage life and stir seed, carrying out 8 times altogether and breathe freely and stir seed, to maintain seed appropriate aqueous amount and enough oxygen, sustain life power.
Through the method for above-mentioned storage, after storing 300 days, splitting mouth shows money or valuables one carries unintentionally about to have 80% seed to occur, proves that the inventive method can extend the Seed storage time and breaking dormancy.
Seed after the inventive method is preserved carries out germination percentage test: the seed preserved after 300 days is placed in and moistening perlitic culture dish is housed, moistening perlitic percent mass moisture content is 90%, adopt 4 culture dishes, 25 seeds are put in each culture dish, sprout at being placed in preference temperature 20 DEG C, germination rate is higher than 85%.Simultaneously, seed after the inventive method is preserved carries out greenhouse pot culture simulated experiment, to detect emergence rate: preserve the seed after 300 days and plant cultivation in soil-vermiculite matrix, use 4 sprouting dishes, 25 seeds are sowed in each dish, sowing depth 1 centimetre of substrate water content is 40%, and emergence rate is higher than 80%, as shown in table 1.Result for detecting each storage time germination rate and emergence rate in seed storage process of the present invention in table 1.As shown in Table 1, the storage of Panax notoginseng seeds of the present invention the method for breaking dormancy, can the storage time of significant prolongation seed, makes the vitality that seed keeps higher, the germination rate after plantation and emergence rate high.
Table 1 stored seeds time and vigour changes
The method of the storage of enforcement 2, Panax notoginseng seeds
Identical with method in the embodiment of the present invention 1; Unlike:
Step 3) in the cryopreserved time be 270, wherein breathed freely every 1 month during stored refrigerated and stir seed, carrying out 9 times altogether and breathe freely and stir seed;
Step 4) in time of stored under refrigeration be 90 days, wherein breathe freely and stir seed week about by the time for duration of storage, carries out 12 times altogether and breathe freely and stir seed.
The total storage time of the inventive method is the seed after 360 days, and after storage, the seed of breaking dormancy carries out germination percentage test according to the potted plant simulation of the embodiment of the present invention 1 medium temperature chamber, and germination rate is 54.67%; Meanwhile, seed carries out according to the potted plant simulation of the embodiment of the present invention 1 medium temperature chamber detection of emerging, and emergence rate is 48%, as shown in table 1.
Claims (10)
1. Panax notoginseng seeds control is wet and is done a method for Tibetan, comprises the steps: 1) seed treatment: the ripening fruits gathering pseudo-ginseng, rub with the hands in water and remove pericarp, obtain the seed of mature and plump, clean with clear water after sterilization;
2) seed drying: by step 1) the described seed that obtains dries in the shade;
3) by step 2) seed that processes mixes with dry perlite, be divided in open-top receptacle, and then described open-top receptacle is placed in closed container controls clammy Tibetan, add water bottom wherein said closed container, make described open-top receptacle not contact described water with band dummy receptacle;
4) by step 3) seed that processes mixes with moistening perlite, then stored refrigerated.
2. method according to claim 1, is characterized in that: step 1) in, the reagent of described sterilization is liquor natrii hypochloritis;
The reagent concentration of described sterilization is 0.1% ~ 2.5%;
The time of described sterilization is 5 ~ 10min.
3. method according to claim 1 and 2, is characterized in that: the water content after described seed dries in the shade is 50 ~ 58%.
4. method according to claim 3, is characterized in that: step 3) in, the perlitic volume ratio of described seed and described drying is 1:0.5 ~ 2;
The temperature of the clammy Tibetan of described control is 2 ~ 4 DEG C, and the time of the clammy Tibetan of described control is 180 ~ 240d, and the relative moisture in described closed container is 98 ~ 100%.
5. the method according to any one of claim 1-4, is characterized in that: step 3) also comprise described open-top receptacle sterilizing and dry and/or carry out breathing freely at least 1 time and the step stirred at the described control During Cold Storage that wets.
6. method according to claim 5, is characterized in that: step 3) in, describedly breathe freely and stir that to carry out number of times be 1 ~ 9 time;
Carry out described to breathe freely and the time interval stirred is 14 ~ 30h.
7. the method according to any one of claim 1-6, is characterized in that: seed and the described moistening perlitic volume ratio of described refrigeration are 1:3 ~ 10.
8. the method according to any one of claim 1-7, is characterized in that: described moistening perlitic water content is 20 ~ 80%.
9. the method according to any one of claim 1-8, is characterized in that: step 4) in, the temperature of described refrigeration is 2 ~ 4 DEG C, and the time of described refrigeration is 60 ~ 90d;
Step 4) be also included in described During Cold Storage and carry out breathing freely at least 1 time and the step stirred.
10. method according to claim 9, is characterized in that: step 4) in, describedly breathe freely and stir that to carry out number of times be 3 ~ 12 times;
Carry out described to breathe freely and the time interval stirred is 7 ~ 30d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510363032.2A CN104956860B (en) | 2015-06-26 | 2015-06-26 | A kind of method of the wet dry Tibetan of Panax notoginseng seeds control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510363032.2A CN104956860B (en) | 2015-06-26 | 2015-06-26 | A kind of method of the wet dry Tibetan of Panax notoginseng seeds control |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104956860A true CN104956860A (en) | 2015-10-07 |
| CN104956860B CN104956860B (en) | 2017-08-08 |
Family
ID=54211101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510363032.2A Active CN104956860B (en) | 2015-06-26 | 2015-06-26 | A kind of method of the wet dry Tibetan of Panax notoginseng seeds control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104956860B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106688368A (en) * | 2016-11-28 | 2017-05-24 | 安徽牧龙山生态旅游开发股份有限公司 | Storage method of dendrobium officinale seeds |
| CN106888619A (en) * | 2017-02-28 | 2017-06-27 | 苏州大学 | A kind of method for improving wild Spartina alterniflora's percentage of seedgermination |
| CN109287189A (en) * | 2018-09-11 | 2019-02-01 | 中国农业大学 | A kind of Panax notoginseng seeds straddle over year the method for preservation and breaking dormancy |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112147A (en) * | 2007-08-30 | 2008-01-30 | 福建师范大学 | Safe hoarding method for Chinese bandoline wood seeds |
| CN102487628A (en) * | 2011-12-13 | 2012-06-13 | 中国科学院生态环境研究中心 | Method for long-term storage and dormancy breaking for seeds of panax notoginseng |
| CN102627494A (en) * | 2012-03-25 | 2012-08-08 | 云南远益园林工程有限公司 | Floating seedling and cultivation medium for pseudo-ginseng seed |
| CN103583108A (en) * | 2013-11-01 | 2014-02-19 | 云南农业大学 | Pseudo-ginseng seedling growing method |
-
2015
- 2015-06-26 CN CN201510363032.2A patent/CN104956860B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112147A (en) * | 2007-08-30 | 2008-01-30 | 福建师范大学 | Safe hoarding method for Chinese bandoline wood seeds |
| CN102487628A (en) * | 2011-12-13 | 2012-06-13 | 中国科学院生态环境研究中心 | Method for long-term storage and dormancy breaking for seeds of panax notoginseng |
| CN102627494A (en) * | 2012-03-25 | 2012-08-08 | 云南远益园林工程有限公司 | Floating seedling and cultivation medium for pseudo-ginseng seed |
| CN103583108A (en) * | 2013-11-01 | 2014-02-19 | 云南农业大学 | Pseudo-ginseng seedling growing method |
Non-Patent Citations (2)
| Title |
|---|
| 段银妹 等: "三七种子的脱水敏感性与膜质过氧化作用", 《种子》 * |
| 萧凤回，文国松: "《云南中药材GAPS实施概论》", 31 October 2005 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106688368A (en) * | 2016-11-28 | 2017-05-24 | 安徽牧龙山生态旅游开发股份有限公司 | Storage method of dendrobium officinale seeds |
| CN106888619A (en) * | 2017-02-28 | 2017-06-27 | 苏州大学 | A kind of method for improving wild Spartina alterniflora's percentage of seedgermination |
| CN109287189A (en) * | 2018-09-11 | 2019-02-01 | 中国农业大学 | A kind of Panax notoginseng seeds straddle over year the method for preservation and breaking dormancy |
| CN109287189B (en) * | 2018-09-11 | 2021-03-09 | 中国农业大学 | Method for storing pseudo-ginseng seeds in trans-year manner and breaking dormancy |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104956860B (en) | 2017-08-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107258148B (en) | A kind of processing method for accelerating apocarya germination | |
| CN104429965B (en) | The method that high eyebrow Herba Anoectochili roxburghii seed is bred without hormone quickly tissue culture | |
| CN106508183A (en) | Germination accelerating method for fructus crataegi seeds | |
| CN102771223B (en) | Piptanthus nepalensis seed preservation and seedling raising methods | |
| CN105284234B (en) | A kind of method for improving sweet cherry percentage of seedgermination | |
| CN102487628A (en) | Method for long-term storage and dormancy breaking for seeds of panax notoginseng | |
| CN104996103B (en) | Pseudo-ginseng seed storage method | |
| CN106852366B (en) | Storage and preservation method of green Chinese onions | |
| CN105850725A (en) | Pitaya pollination method | |
| CN106717266A (en) | The long-term preservation method of dendrobium candidum capsule/seed | |
| CN101112147B (en) | Safe hoarding method for Chinese bandoline wood seeds | |
| CN104956860B (en) | A kind of method of the wet dry Tibetan of Panax notoginseng seeds control | |
| CN102498783B (en) | Wild cryptotaenia japonica seed dormancy breaking method | |
| CN103444309A (en) | Method for quickly germinating paris polyphylla var.chinensis seeds through combination of gibberellin and filter paper | |
| CN105493683B (en) | A kind of processing method after roxburgh anoectochilus terminal bud seed collection | |
| CN108668557A (en) | It is a kind of to utilize method of germination corn enrichment lutein and products thereof and application | |
| CN107810958A (en) | A kind of method for promoting Yunnan Paris polyphylla seed to sprout | |
| CN109526298B (en) | Passion fruit seed treatment method and sowing and breeding method thereof | |
| CN104938088B (en) | Method for promoting germination of caulis sinomenii seeds | |
| CN105027729B (en) | The method of the wet dry Tibetan of Panax notoginseng seeds control | |
| CN204762296U (en) | A isolated plant that is used for wet storage of pseudo -ginseng seed accuse | |
| CN114451240B (en) | Method for promoting emergence of polygonatum odoratum seeds | |
| CN105961376A (en) | Storage method for keeping pitaya pollen activity | |
| CN107155445B (en) | Method for improving endosperm activity of gynura divaricata seeds after low-temperature storage | |
| CN105494612A (en) | Method for inhibiting stems of stored grapes from browning and fruit particles from falling off |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |