CN104904757B - A kind of organic vegetable pours root agent and its application method - Google Patents
A kind of organic vegetable pours root agent and its application method Download PDFInfo
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Abstract
Root agent and its application method are poured the invention discloses a kind of organic vegetable, described root agent of pouring is formed by tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag compatibility, its tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0~3.0:1.0~2.0:0.5~1.0 compatibility mixture, is preferably in a proportion of 2.5:1.5:0.75, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is calculated by the dry cake weight of 1~30d moisture absorption of not going mouldy, and leached tea oil slag need to first press leached tea oil slag:Water is 1:10 ratio, which adds water, is made leached tea oil slag zymotic fluid, and its zymotic fluid is that auxiliary agent doubles as brewed medium, and the brewed liquid of this compatibility is stoste.This compatibility agent is notable to the prevention effect of organic vegetable root-knot nematode, and with synergy.Before root knot nematode outburst or when breaking out, by every 667m25~15kg stostes, 15 times of liquid, which are poured, to be applied, and can comparatively fast be killed root-knot nematode, be cut off extension, and accelerates the recovery of plant.Originally root agent production is poured upper easy to use, simple to operate, cost is low, and prevention effect is notable.
Description
Technical field
The invention belongs to agriculture practical new technology research and development and application；The present invention relates to the research and development that a kind of organic vegetable pours root agent
With production application method.
Background technology
Root knot nematode Meloidogynespp, Root-knot nematodes) parasitize in root system of plant
Obligatory parasitism is biological, with host range extensively, strong adaptability, the features such as fertility is strong, to modern agricultural production and development
Cause than large effect and harm.With the increase of China's vegetable cultivation area, the generation of root knot nematode disease adds year by year
Weight, after disease occurs, typically results in yield and reduces 20%-30%, more than 75%, or even total crop failure are reached when serious.United according to related data
Meter, the crops economic loss of the whole world every year caused by root-knot nematode endangers is about more than 100,000,000,000 dollars.While root knot
Nematodiasis has aggravated the generation of silborne fungal diseases, such as：Droop, root rot.2011 in Macheng City of Hubei Province Song Bu towns
Vegetable Base finds that various vegetables crop has all infected root-knot nematode, causes serious economic loss.
Root knot nematode disease be often occur on vegetables have a strong impact on one of disease of yield.Beans, melon, capsicum class, eggplant
The vegetables such as fruit, vegetables with green leaves class, root vegetables and green onion garlic class are nearly all its hosts, and production loss is seriously even had no harvest, together
When can aggravate the generation of various native the transmissibility fungal diseases and part bacterial disease such as droop, root rot again.
At present, mainly there are physical control, chemical prevention, biological control etc. to the prevention and controls of root knot nematode disease.Thing
Reason preventing and treating such as carries out soil-fumigating in batch phase of having a rest, and chemical prevention such as controls line treasured using chemical agent AVM hereinafter, and biological control is as selected
Educate various microbial inoculums, screening disease-resistant variety etc..Physical control is time-consuming to take a lot of work, and biological control does not select high-effect bacterial also, and chemistry is anti-
Though controlling existing good chemical agent, the production of organic vegetable can not use chemical agent, forbid to make in organic vegetable production
With, therefore limit it and use.This organic vegetable pours root agent and overcomes disadvantages described above, both pollution-free, there is preventing and treating effect well again
Really, raw material is derived from naturally, wide material sources, cost is low, is that a kind of good organic vegetable pours root agent.Disease is used before occurring, energy
Prevention occurs；Applied after generation, can comparatively fast kill rhizosphere nematode, prevent nematode from spreading, plant restore normal growth soon.This is organic
It is that, through studying the achievement with production application for many years, it can both prevent that vegetables, which pour root agent, can be controlled again, and easy to use, simple to operate, into
This is low, and significantly, this is other can not be than about medicament or measure for prevention effect.
The content of the invention
The purpose of the present invention aims to provide a kind of organic vegetable and pours root agent, easy to use, and simple to operate, cost is low, preventing and treating
Effect is notable.Another object of the present invention aims to provide the preferably application method that above-mentioned organic vegetable pours root agent.The present invention's
Purpose is achieved in the following ways：
The control agent of the present invention is tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag composition, and wherein leached tea oil slag is mainly used as helping
Agent, plays a part of to promote its prevention effect, as brewed medium.Inventor's research finds that adding auxiliary agent leached tea oil slag can play very
Good synergistic effect.Need it is emphasized that the tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag that this organic vegetable pours root agent all should
From from the free of contamination area of the environmental conditions such as soil, water, air, soil, water body and organic fertilizer etc. also want heavy metal free to pollute,
Tripterygium wilfordii, polygonum flaccidum, tea oil tree system grow naturally to be formed, and the mountain area of chemical fertilizer, chemical pesticide was not applied.Otherwise, it will influence organic
The quality of vegetables.The preparation method that this organic vegetable pours root agent is：Material tripterygium leaf, polygonum flaccidum leaf, leached tea oil slag all should be from next
From the free of contamination area of the environmental conditions such as soil, water, air, tripterygium leaf, polygonum flaccidum leaf use fresh and alive material, and leached tea oil slag is 1-30d
Moisture absorption of not going mouldy dry cake.First the soil on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, washing is not needed.With hammer tea seed
The dregs of rice are smashed to pieces, by leached tea oil slag:Water=1:10 ratio is soaked in water, after mixing after capping fermentation 15-20d, 15-20d, Thunder God
Rattan fresh leaf, polygonum flaccidum fresh leaf are put into and wherein soak 3-5d.Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is by 1-30d's
Do not go mouldy moisture absorption dry cake weight calculate.Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of the component of leached tea oil slag three are 2.0~3.0:
1.0~2.0:0.5~1.0, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is by the dry of 1-30d moisture absorption of not going mouldy
Cake weight is calculated.Smash leached tea oil slag to pieces:Water=1:10 ratio is soaked in water, after mixing after capping fermentation 15-20d, 15-20d,
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are put into and wherein soak 3-5d, after 3-5d, 4 layers of filtered through gauze 2~3 times is collected filtrate, be put into
Container, is sealed immediately, is that this organic vegetable pours root agent stoste（That is mother liquor）, can preserve 1-6 months.This organic vegetable is poured
Root agent is applied before disease occurs, and prevention is pressed per 667m25~10kg stostes, 15 times of liquid, which are poured, to be applied（The sprayer for spending shower nozzle is attacked a vital point
Pour and apply more preferably）；Applied after insect pest generation, by every 667m210~15kg stostes, 15 times of liquid, which are poured, to be applied（Spend the sprayer of shower nozzle
Attack a vital point to pour and apply more preferably）, rhizosphere insect can be comparatively fast killed, prevents insect pest from spreading.The production of this control agent is upper easy to use, operation letter
Single, cost is low, and significantly, the shelf-life is longer, to stoste for prevention effect（That is mother liquor）Packed with plastic bag sealing, batch production can be carried out
Production, forms product.
The component tripterygium wilfordii fresh leaf of the above three, polygonum flaccidum fresh leaf, the most preferred mass ratio of leached tea oil slag are 2.5:1.5:0.75, thunder
Public rattan fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy.Present invention choosing
With control agent tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf and auxiliary agent leached tea oil slag, the compatibility of suitable concentration is employed, commercial preventing and treating is configured to
Agent, it can both prevent, can control again, and it has significant preventive and therapeutic effect to organic vegetable root-knot nematode.Root is poured using this organic vegetable
Agent is it compared with one of effective measures.This control agent is easy to use, simple to operate, and cost is low, and significantly, this is other to prevention effect
Relevant medicament or measure can not than.This organic vegetable pours root agent prescription for tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf and auxiliary agent tea seed
The dregs of rice, are mixed by the ratio of above-mentioned mass ratio and are preserved at brewed, mother liquor plastic bag sealing packaging, the dry lucifuge in cool place, the shelf-life 6
Month.Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy.Below
It is the comparative test of its regulating effect.The organic vegetable of the present invention pours the report that root agent has no like product at present, and the present invention is
The result of the test that inventor is drawn by experimental study for many years and production practices, and tested in the production of long period
Card, effect is preferable.And the formula of the present invention also has technology content higher, formulation is relatively stablized, and raw material sources enrich, operation letter
Just, commodity are such as developed into, it is cheap.Easy to use in production, significantly, use cost is low for prevention effect, is a kind of
Good organic vegetable pours root agent.Organic vegetable, which pours root agent prescription, can carry out factorial praluction, and its exploitation not only will be in city
Increase a kind of new organic vegetable on and pour root agent, will also be that the problem of current organic vegetable root knot nematode control effect is not good carries
For the approach of a solution.The present invention application method be：This organic vegetable pours root agent and applied before root-knot nematode occurs, prevention
By every 667m25~10kg stostes, 15 times of liquid, which are poured, to be applied；Applied after root-knot nematode occurs, by every 667m210~15kg is former
15 times of liquid of liquid, which are poured, to be applied, and can comparatively fast kill root-knot nematode, prevents nematode from spreading.
Using can not sprinkle water or apply fertilizer in rear 24h, conventional rich water quality management after 24h, if in using having been descended within rear 24h
To heavy rain, then need to handle again after rain.Experiment shows, after 15d, and vegetables are detected, the major function of its medicament
Composition is not detected, does not during which descend moderate rain or heavy rain；After, during which following moderate rain or heavy rain more than 1 time, after 7d, its main work(
Energy composition is not detected.
The purpose of the present invention is realized the following is inventor, is constantly selected and is had from suitable of the invention by experiment
Machine vegetables pour the comparative test for the prevention effect that the raw material of root agent prescription and its proportioning of correlation are done.
Single group separating tests
Single group separating tests are mainly the single effect that each component of qualitative determination is prevented and treated organic vegetable root-knot nematode
Fruit is tested.
Materials and methods
1.1.1 test material
Experiment vegetable species are from capsicum, cucumber, the four kinds of vegetables of tomato and wild cabbage for being easiest to break out root-knot nematode.Experiment
It is with ingredients：Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag.
Test method
The preparation of test material：Material tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag, all should select come from soil, water, air
Etc. the free of contamination area of environmental condition, soil, water body and organic fertilizer etc. also want heavy metal free to pollute, tripterygium wilfordii, polygonum flaccidum, tea oil tree
It is that nature growth is formed, the mountain area of chemical fertilizer, chemical pesticide was not applied.Tripterygium leaf, polygonum flaccidum leaf use fresh and alive material, leached tea oil slag
For the dry cake of 1-30d moisture absorption of not going mouldy.First the soil on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, washing is not needed.With hammer
Leached tea oil slag is smashed to pieces, by leached tea oil slag:Water=1:10 ratio is soaked in water, after mixing after capping fermentation 15-20d, 15-20d,
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are soaked in the ratio of next segment description respectively and are wherein immersed in 3- in leached tea oil slag zymotic fluid
5d.Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy.Thunder God
Rattan fresh leaf component prepares mother liquor by following mass ratio：Tripterygium wilfordii fresh leaf:Leached tea oil slag zymotic fluid=2.5:5.0（Wherein leached tea oil slag ferments
Liquid is brewed medium, and its quality is included into its mother liquor gross mass）.Polygonum flaccidum fresh leaf component prepares mother liquor by following mass ratio, and polygonum flaccidum is fresh
Leaf:=1.5:5.0（Wherein leached tea oil slag zymotic fluid is brewed medium, and its quality is included into its mother liquor gross mass）.Leached tea oil slag component is by such as
Lower mass ratio prepares mother liquor, leached tea oil slag:Water=1.0:10.0（Its reclaimed water is brewed medium, and its quality is included into its mother liquor gross mass）.
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy.Capping leaching
Steep after 3-5d, 4 layers of filtered through gauze 2~3 times collects filtrate, be put into container, seal immediately, as the mother of each component single dose
Stoste, can be preserved 1-6 months.When breaking out root-knot nematode, lid is opened, each single dose stoste is taken out respectively（Component is taken out
Deposit 30d's）, by every 667m25th, 10,15 times of liquid of 3 concentration gradients of 15kg stostes（Refer to amount of water）Spend the spraying of shower nozzle
Device is attacked a vital point to pour and applied.
Experiment is carried out in Yiyang proving ground.The plot that root-knot nematode is often sent out from former years is carried out.When capsicum, cucumber, kind
Prevention effect experiment is carried out when eggplant occurs with wild cabbage root-knot nematode；Control is located at same plot.Test effect is mainly with same
When kind vegetable seedling new root occurs slow, pour after dispenser agent, counted with the percentage of root knot quantity reduction and statistical analysis, it is right
According to（CK）Poured and applied with water spot cave.Capsicum, three kinds of vegetables of cucumber and tomato are when root-knot nematode occurs, and selection is entered at cloudy day or fine day
Row processing.Each single dose amount of application：Its mother liquor amount of application of tripterygium wilfordii fresh leaf component is pressed per 667m2Tri- kinds of 5kg, 10kg, 15kg
Concentration gradient carries out pouring administration.Its mother liquor amount of application of polygonum flaccidum fresh leaf component is pressed per 667m2Tri- kinds of concentration of 5kg, 10kg, 15kg
Gradient carries out pouring administration.Its mother liquor amount of application of leached tea oil slag component is pressed per 667m2Tri- kinds of concentration gradients of 5kg, 10kg, 15kg are entered
Row pours administration.Pour to apply and do not water or apply fertilizer in processing 24h, observed and counted during 15d.Conventional fertilizer water pipe after 24h
Reason.Experiment sets 36 processing, 4 controls, each 3 repetitions of processing, each repeatedly 1.0m2, random distribution.With dead percentage
Treatment effect is used for, in combination with field observation.The disposition of each vegetables is shown in the following description to each processing.
Processing 1：The test dose of capsicum --- tripterygium wilfordii fresh leaf is pressed per 667m25kg is applied；Processing 2：The experiment of capsicum
Dosage --- tripterygium wilfordii fresh leaf is pressed per 667m210kg is applied；Processing 3：The test dose of capsicum --- tripterygium wilfordii fresh leaf is by every
667m215kg is applied；Processing 4：The test dose of capsicum --- polygonum flaccidum fresh leaf is pressed per 667m25kg is applied；Processing 5：Capsicum
Test dose --- polygonum flaccidum fresh leaf is pressed per 667m210kg is applied；Processing 6：The test dose of capsicum --- polygonum flaccidum fresh leaf is by every
667m215kg is applied；Processing 7：The test dose of capsicum --- leached tea oil slag is pressed per 667m25kg is applied；Processing 8：The examination of capsicum
Test dosage --- leached tea oil slag is pressed per 667m210kg is applied；Processing 9：The test dose of capsicum --- leached tea oil slag is pressed per 667m2
15kg is applied；CK1：The control of capsicum, is poured with water spot cave and applied.Processing 10：The test dose of cucumber --- tripterygium wilfordii fresh leaf is by every
667m25kg is applied；Processing 11：The test dose of cucumber --- tripterygium wilfordii fresh leaf is pressed per 667m210kg is applied；Processing 12：It is yellow
The test dose of melon --- tripterygium wilfordii fresh leaf is pressed per 667m215kg is applied；Processing 13：The test dose of cucumber --- polygonum flaccidum is fresh
Leaf is pressed per 667m25kg is applied；Processing 14：The test dose of cucumber --- polygonum flaccidum fresh leaf is pressed per 667m210kg is applied；Processing
15：The test dose of cucumber --- polygonum flaccidum fresh leaf is pressed per 667m215kg is applied；Processing 16：The test dose of cucumber --- tea seed
The dregs of rice are pressed per 667m25kg is applied；Processing 17：The test dose of cucumber --- leached tea oil slag is pressed per 667m210kg is applied；Processing 18：
The test dose of cucumber --- leached tea oil slag is pressed per 667m215kg is applied；CK2：The control of cucumber, is poured with water spot cave and applied.Processing
19：The test dose of tomato --- tripterygium wilfordii fresh leaf is pressed per 667m25kg is applied；Processing 20：The test dose of tomato --- thunder
Public rattan fresh leaf is pressed per 667m210kg is applied；Processing 21：The test dose of tomato --- tripterygium wilfordii fresh leaf is pressed per 667m215kg is applied
With；Processing 22：The test dose of tomato --- polygonum flaccidum fresh leaf is pressed per 667m25kg is applied；Processing 23：The experiment agent of tomato
Amount --- polygonum flaccidum fresh leaf is pressed per 667m210kg is applied；Processing 24：The test dose of tomato --- polygonum flaccidum fresh leaf is pressed per 667m2
15kg is applied；Processing 25：The test dose of tomato --- leached tea oil slag is pressed per 667m25kg is applied；Processing 26：The experiment agent of tomato
Amount --- leached tea oil slag is pressed per 667m210kg is applied；Processing 27：The test dose of tomato --- leached tea oil slag is pressed per 667m215kg is applied
With；CK3：The control of tomato, is poured with water spot cave and applied.Processing 28：The test dose of wild cabbage --- tripterygium wilfordii fresh leaf is pressed per 667m2
5kg is applied；Processing 29：The test dose of wild cabbage --- tripterygium wilfordii fresh leaf is pressed per 667m210kg is applied；Processing 30：The examination of wild cabbage
Test dosage --- tripterygium wilfordii fresh leaf is pressed per 667m215kg is applied；Processing 31：The test dose of wild cabbage --- polygonum flaccidum fresh leaf is by every
667m25kg is applied；Processing 32：The test dose of wild cabbage --- polygonum flaccidum fresh leaf is pressed per 667m210kg is applied；Processing 33：Wild cabbage
Test dose --- polygonum flaccidum fresh leaf is pressed per 667m215kg is applied；Processing 34：The test dose of wild cabbage --- leached tea oil slag is by every
667m25kg is applied；Processing 35：The test dose of wild cabbage --- leached tea oil slag is pressed per 667m210kg is applied；Processing 36：Wild cabbage
Test dose --- leached tea oil slag is pressed per 667m215kg is applied；CK4：The control of wild cabbage, is poured with water spot cave and applied.
Statistical analysis
SAS is used to test data（6.12）Carry out statistical analysis.The effect each handled during processing 15d is shown in Table 1-2.Examination
Result is tested to show（Table 1）, processing 3 root knot number slip be significantly higher than processing 1, illustrate higher concentration tripterygium wilfordii fresh leaf prevent and treat it is peppery
The effect of green pepper root-knot nematode is significantly better than the tripterygium wilfordii fresh leaf of low concentration.Processing 1-3 is significantly higher than other processing again on the whole
With CK1, i.e. the effect of tripterygium wilfordii fresh leaf preventing and treating capsicum root-knot nematode is significantly better than polygonum flaccidum fresh leaf, the prevention effect of leached tea oil slag.No
With concentration polygonum flaccidum fresh leaf prevent and treat capsicum root-knot nematode effect be：Processing 5-6 root knot number slip is significantly higher than processing 4, says
The effect of bright higher concentration polygonum flaccidum fresh leaf preventing and treating capsicum root-knot nematode is significantly better than the polygonum flaccidum fresh leaf of low concentration.Handle 4-6 again
It is significantly higher than processing 7-12 and CK1, i.e. the effect of polygonum flaccidum fresh leaf preventing and treating capsicum root-knot nematode is significantly better than leached tea oil slag prevention effect,
But not as good as the prevention effect of tripterygium wilfordii fresh leaf.Various concentrations leached tea oil slag preventing and treating capsicum root-knot nematode effect be：Higher concentration
The processing 7-8 root knot number slips of processing 9 and low concentration are without significant difference, but processing 7-9 is significantly higher than CK1, i.e. leached tea oil slag
There is the effect of certain preventing and treating to capsicum root-knot nematode, but not as good as the prevention effect of tripterygium wilfordii fresh leaf and polygonum flaccidum fresh leaf.
Capsicum during 1. one pack system test process 15d of table, the root knot number slip on cucumber（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 1 | 30.20±3.40c | Processing 10 | 28.23±2.75b |
| Processing 2 | 40.87±2.03ab | Processing 11 | 33.21±2.15ab |
| Processing 3 | 49.79±1.76a | Processing 12 | 36.90±1.90a |
| Processing 4 | 25.11±0.86d | Processing 13 | 21.30±3.64c |
| Processing 5 | 38.94±3.52b | Processing 14 | 34.09±2.22ab |
| Processing 6 | 32.67±1.70bc | Processing 15 | 38.35±2.81a |
| Processing 7 | 6.20±3.07e | Processing 16 | 3.49±1.85d |
| Processing 8 | 8.89±1.54e | Processing 17 | 5.90±0.90d |
| Processing 9 | 9.21±0.56e | Processing 18 | 7.32±2.01d |
| CK1 | 1.69±0.87f | CK2 | 0.52±0.28e |
Note：Lowercase is 0.05 level, and the different letters of same row represent significant difference, similarly hereinafter.
Various concentrations tripterygium wilfordii fresh leaf preventing and treating cucumber root-knot nematode effect be：The root knot number slip of processing 12 is significantly high
In processing 10, illustrate that the effect of higher concentration tripterygium wilfordii fresh leaf preventing and treating cucumber root-knot nematode is significantly better than the tripterygium wilfordii of low concentration
Fresh leaf.Processing 10-12 is significantly higher than processing 16-18 and CK2 again, i.e. the effect of tripterygium wilfordii fresh leaf preventing and treating cucumber root-knot nematode is notable
Better than the prevention effect of leached tea oil slag.Various concentrations polygonum flaccidum fresh leaf preventing and treating cucumber root-knot nematode effect be：The root knot number of processing 15
Slip is significantly higher than processing 13, illustrate higher concentration polygonum flaccidum fresh leaf preventing and treating cucumber root-knot nematode effect be significantly better than it is relatively low dense
The polygonum flaccidum fresh leaf of degree.Processing 13-15 is significantly higher than processing 16-18 and CK2, i.e. polygonum flaccidum fresh leaf and prevents and treats the effect of capsicum root-knot nematode
It is significantly better than the prevention effect of leached tea oil slag.Various concentrations leached tea oil slag preventing and treating cucumber root-knot nematode effect be：Handle 16-18 root
Tie number slip without significant difference, but with CK2 significant differences.I.e. leached tea oil slag preventing and treating capsicum root-knot nematode has certain effect,
But not as good as the prevention effect of tripterygium wilfordii fresh leaf and polygonum flaccidum fresh leaf.
Result of the test shows（Table 2）, the root knot number slip of processing 27, which is significantly higher than, handles 25-26, illustrates higher concentration
The effect of tripterygium wilfordii fresh leaf preventing and treating tomato root-knot eelworm is significantly better than the tripterygium wilfordii fresh leaf of low concentration.Handle 25-27 again notable
Effect higher than rhizosphere insect on other processing and CK3, i.e. tripterygium wilfordii fresh leaf preventing and treating tomato is significantly better than polygonum flaccidum fresh leaf, tea seed
The dregs of rice, prevention effect.
Various concentrations polygonum flaccidum fresh leaf preventing and treating tomato root-knot eelworm effect be：The root knot number of the processing 24 of higher concentration is reduced
Rate is significantly higher than the processing 22 of low concentration.But processing 22-24 root knot number slip is significantly higher than processing 25-27 and CK3, i.e.,
The effect of polygonum flaccidum fresh leaf preventing and treating tomato root-knot eelworm is significantly better than the prevention effect of leached tea oil slag.Various concentrations leached tea oil slag prevents and treats tomato
The effect of root-knot nematode is：The processing 27 of higher concentration and the processing 25-26 root knot number slips of low concentration without significant difference,
But processing 25-27 is significantly higher than CK3, i.e., the effect of leached tea oil slag preventing and treating tomato root-knot eelworm significantly, but not as good as tripterygium wilfordii fresh leaf with
The prevention effect of polygonum flaccidum fresh leaf.
Tomato during 2. one pack system test process 15d of table, the root knot number slip on wild cabbage（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 19 | 26.76±2.23b | Processing 28 | 25.78±0.95b |
| Processing 20 | 30.81±0.67ab | Processing 29 | 31.27±1.34a |
| Processing 21 | 35.60±3.97a | Processing 30 | 32.38±1.66a |
| Processing 22 | 20.19±2.55c | Processing 31 | 18.26±5.35c |
| Processing 23 | 27.80±3.10b | Processing 32 | 21.45±3.21bc |
| Processing 24 | 31.65±1.51ab | Processing 33 | 29.03±0.82a |
| Processing 25 | 6.91±0.80d | Processing 34 | 5.33±1.54d |
| Processing 26 | 8.44±1.69d | Processing 35 | 7.51±0.69d |
| Processing 27 | 9.07±2.92d | Processing 36 | 9.35±2.13d |
| CK3 | 0.80±0.52e | CK4 | 0.02±0.01e |
The effect of root-knot nematode is on various concentrations tripterygium wilfordii fresh leaf preventing and treating wild cabbage：Handle 29-30 root knot number slip
It is significantly higher than processing 28, illustrates that the effect of root-knot nematode on higher concentration tripterygium wilfordii fresh leaf preventing and treating wild cabbage is significantly better than low concentration
Tripterygium wilfordii fresh leaf.Processing 29-30 is significantly higher than root-knot nematode on processing 34-36 and CK4, i.e. tripterygium wilfordii fresh leaf preventing and treating wild cabbage again
Effect be significantly better than the prevention effect of leached tea oil slag.The effect of root-knot nematode is on various concentrations polygonum flaccidum fresh leaf preventing and treating wild cabbage：Place
The root knot number slip of reason 33 is significantly higher than processing 31-32, illustrates root-knot nematode on higher concentration polygonum flaccidum fresh leaf preventing and treating wild cabbage
Effect is significantly better than the polygonum flaccidum fresh leaf of low concentration.Processing 31-33, which is significantly higher than processing 34-36 and CK4, i.e. polygonum flaccidum fresh leaf, to be prevented and treated
The effect of root-knot nematode is significantly better than the prevention effect of leached tea oil slag on wild cabbage.Root-knot nematode on various concentrations leached tea oil slag preventing and treating wild cabbage
Effect be：Processing 34-36 root knot number slip is significantly higher than CK4, but not as good as the preventing and treating of tripterygium wilfordii fresh leaf and polygonum flaccidum fresh leaf
Effect.
Shown by above single group separating tests, higher concentration tripterygium wilfordii fresh leaf preventing and treating capsicum, cucumber, tomato, on wild cabbage
Its effect of root-knot nematode is attained by remarkable result.Tripterygium wilfordii fresh leaf, the effect of polygonum flaccidum fresh leaf are preferably, secondly leached tea oil slag.With
The reduction of concentration, prevention effect is remarkably decreased.Single group separating tests are also shown that each component is used alone, and its prevention effect is low
In 50%.
Compatibility mechanism
Compatibility mechanism is mainly qualitative and quantitative and determines the effect that each ingredients is prevented and treated organic vegetable rhizosphere insect
Fruit is tested.
Materials and methods
2.1.1 test material
Experiment vegetable species are from capsicum, cucumber, the four kinds of vegetables of tomato and wild cabbage for being easiest to break out root-knot nematode.Experiment
It is with ingredients：Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag.
Test method
The preparation of test material：Material tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag all should be selected from soil, water, air etc.
The free of contamination area of environmental condition, soil, water body and organic fertilizer etc. also want heavy metal free to pollute, tripterygium wilfordii, polygonum flaccidum, tea oil tree system
Naturally growth is formed, and the mountain area of chemical fertilizer, chemical pesticide was not applied.Tripterygium leaf, polygonum flaccidum leaf use fresh and alive material, and leached tea oil slag is
The dry cake of 1-30d moisture absorption of not going mouldy.First the soil on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, washing is not needed.With hammer handle
Leached tea oil slag is smashed to pieces, by leached tea oil slag:Water=1:10 ratio is soaked in water, after mixing after capping fermentation 15-20d, 15-20d,
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are soaked in 3-5d in leached tea oil slag zymotic fluid in the ratio of next segment description respectively.Tripterygium wilfordii fresh leaf,
Polygonum flaccidum fresh leaf is basis by fresh, and leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy.Tripterygium wilfordii fresh leaf, polygonum flaccidum are fresh
Leaf, the mass ratio of the component of leached tea oil slag three are 2.0~3.0:1.0~2.0:0.5~1.0, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf press fresh weight
Calculate, leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, and leached tea oil slag is auxiliary agent.After capping immersion 3-5d, 4 layers
Filtered through gauze 2~3 times, collects filtrate, is put into container, seals immediately, as mother liquor（Stoste）, can preserve 1-6 months.
When breaking out root-knot nematode, lid is opened, stoste is taken out, by every 667m25th, 10,15 times of liquid of 3 concentration gradients of 15kg stostes
（Refer to amount of water）The sprayer for spending shower nozzle is attacked a vital point to pour and applied.Experiment is carried out in Yiyang proving ground.From former years Chang Fagen tie lines
The plot of worm is carried out.Prevention effect experiment is carried out when capsicum, cucumber, tomato and wild cabbage root-knot nematode occur；Control is located at same
One plot.When test effect mainly occurs with same root knot nematode, pour after dispenser agent, hundred reduced with root knot quantity
Point than counting and statistical analysis, control（CK）Poured and applied with water spot cave.
Capsicum, cucumber, four kinds of vegetables of tomato and wild cabbage are when root-knot nematode is broken out, and selection carries out dispenser at cloudy day or fine day
Processing.Pour to apply and do not water or apply fertilizer in processing 24h, observed and counted during 15d.Conventional rich water quality management after 24h.Experiment
36 processing, 4 controls are set, each 3 repetitions of processing, each repeatedly 1.0m2, random distribution.Percentage is reduced with root knot quantity
Treatment effect is used for, in combination with field observation.The disposition of each vegetables is shown in the following description to each processing.Processing
1：The test dose of capsicum --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5 presses per 667m2
5kg is applied；Processing 2：The test dose of capsicum --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:
0.5 presses per 667m210kg is applied；Processing 3：The test dose of capsicum --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the matter of leached tea oil slag
Amount is than being 2.0:1.0:0.5 presses per 667m215kg is applied；Processing 4：The test dose of capsicum --- tripterygium wilfordii fresh leaf, polygonum flaccidum are fresh
Leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m25kg is applied；Processing 5：The test dose of capsicum --- Thunder God
Rattan fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m210kg is applied；Processing 6：The examination of capsicum
Test dosage --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m215kg is applied；
Processing 7：The test dose of capsicum --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:1.0 by every
667m25kg is applied；Processing 8：The test dose of capsicum --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are
3.0:2.0:1.0 press per 667m210kg is applied；Processing 9：The test dose of capsicum --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, tea
The mass ratio of the seed dregs of rice is 3.0:2.0:1.0 press per 667m215kg is applied；CK1：The control of capsicum is poured with water spot cave and applied.Processing
10：The test dose of cucumber --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5 presses per 667m2
5kg is applied；Processing 11：The test dose of cucumber --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:
0.5 presses per 667m210kg is applied；Processing 12：The test dose of cucumber --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the matter of leached tea oil slag
Amount is than being 2.0:1.0:0.5 presses per 667m215kg is applied；Processing 13：The test dose of cucumber --- tripterygium wilfordii fresh leaf, polygonum flaccidum
Fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m25kg is applied；Processing 14：The test dose of cucumber ---
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m210kg is applied；Processing 15：It is yellow
The test dose of melon --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m2
15kg is applied；Processing 16：The test dose of cucumber --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 3.0:
2.0:1.0 press per 667m25kg is applied；Processing 17：The test dose of cucumber --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag
Mass ratio be 3.0:2.0:1.0 press per 667m210kg is applied；Processing 18：The test dose of cucumber --- tripterygium wilfordii fresh leaf,
Polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:1.0 press per 667m215kg is applied；CK2：Water spot cave is used in the control of cucumber
Pour and apply.Processing 19：The test dose of tomato --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5
By every 667m25kg is applied；Processing 20：The test dose of tomato --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag
For 2.0:1.0:0.5 presses per 667m210kg is applied；Processing 21：The test dose of tomato --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf,
The mass ratio of leached tea oil slag is 2.0:1.0:0.5 presses per 667m215kg is applied；Processing 22：The test dose of tomato --- tripterygium wilfordii
Fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m25kg is applied；Processing 23：The experiment of tomato
Dosage --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m210kg is applied；Place
Reason 24：The test dose of tomato --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 by every
667m215kg is applied；Processing 25：The test dose of tomato --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are
3.0:2.0:1.0 press per 667m25kg is applied；Processing 26：The test dose of tomato --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, tea
The mass ratio of the seed dregs of rice is 3.0:2.0:1.0 press per 667m210kg is applied；Processing 27：The test dose of tomato --- tripterygium wilfordii is fresh
Leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:1.0 press per 667m215kg is applied；CK3：The control water spot of tomato
Cave, which is poured, applies.Processing 28：The test dose of wild cabbage --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:
0.5 presses per 667m25kg is applied；Processing 29：The test dose of wild cabbage --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the matter of leached tea oil slag
Amount is than being 2.0:1.0:0.5 presses per 667m210kg is applied；Processing 30：The test dose of wild cabbage --- tripterygium wilfordii fresh leaf, polygonum flaccidum
Fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5 presses per 667m215kg is applied；Processing 31：The test dose of wild cabbage ---
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m25kg is applied；Processing 32：Wild cabbage
Test dose --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag mass ratio be 2.5:1.5:0.75 presses per 667m2 10kg
Using；Processing 33：The test dose of wild cabbage --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:
0.75 presses per 667m215kg is applied；Processing 34：The test dose of wild cabbage --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag
Mass ratio is 3.0:2.0:1.0 press per 667m25kg is applied；Processing 35：The test dose of wild cabbage --- tripterygium wilfordii fresh leaf, polygonum flaccidum
Fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:1.0 press per 667m210kg is applied；Processing 36：The test dose of wild cabbage ---
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:1.0 press per 667m215kg is applied；CK4：Wild cabbage
Control is poured with water spot cave and applied.
Statistical analysis
SAS is used to test data（6.12）Carry out statistical analysis.It was found from table 3-4, processing 1-9 preventing and treating root-knot nematodes
Effect is significantly better than CK1, and the effect for handling 4-6 is significantly better than processing 1-3 again on the whole, with processing 7-9 on the whole without significance difference
Different, i.e., tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 compatibility is pressed per 667m25-15kg is applied
It is 2.0 that prevention effect, which is significantly better than tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag,:1.0:0.5 compatibility is by every
667m2The prevention effect that 5-15kg is applied, i.e. tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5
Compatibility press per 667m25kg administrations are the lower limits of its compatibility concentration.Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag
For 2.5:1.5:The mass ratio of 0.75 compatibility and tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag is 3.0:2.0:1.0 compatibility its
Mass ratio of the prevention effect on the whole without significant difference, i.e. tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag is 3.0:2.0:1.0
Compatibility is pressed per 667m215kg administrations are the upper limits of its compatibility concentration.Accounted in terms of cost and prevention effect 2, Thunder God
Rattan fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 compatibility, its is cost-effective, and raw material, usage amount are fitted
In, and prevention effect is again good, is preferable optimization formula ratio and amount of application.
Capsicum during 3. ingredients test process 15d of table, the root knot number slip on cucumber（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 1 | 76.03±2.30c | Processing 10 | 73.24±3.37c |
| Processing 2 | 81.87±3.18bc | Processing 11 | 79.51±3.31bc |
| Processing 3 | 84.79±0.83b | Processing 12 | 83.90±1.58b |
| Processing 4 | 86.27±3.09b | Processing 13 | 85.55±2.91b |
| Processing 5 | 95.26±1.42ab | Processing 14 | 96.09±3.45a |
| Processing 6 | 97.21±4.10a | Processing 15 | 95.62±2.58a |
| Processing 7 | 94.35±1.82ab | Processing 16 | 91.22±0.70ab |
| Processing 8 | 95.80±0.64ab | Processing 17 | 95.05±3.11a |
| Processing 9 | 98.23±3.25a | Processing 18 | 97.41±1.93a |
| CK1 | 1.69±0.27d | CK2 | 0.52±0.14d |
The effect of processing 10-18 preventing and treating cucumber root-knot nematodes is significantly better than CK2, and the effect for handling 13-15 is again aobvious on the whole
Write better than processing 10-12, with processing 16-18 on the whole without significant difference, i.e. tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the matter of leached tea oil slag
Amount is than being 2.5:1.5:0.75 compatibility is pressed per 667m2The prevention effect that 5-15kg is applied is significantly better than tripterygium wilfordii fresh leaf, polygonum flaccidum
Fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5 compatibility is pressed per 667m2The prevention effect that 5-15kg is applied, i.e. tripterygium wilfordii
Fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5 compatibility is pressed per 667m2It is its compatibility concentration that 5kg, which is applied,
Lower limit.Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:It is 0.75 compatibility and tripterygium wilfordii fresh leaf, peppery
Knotweed fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:Its prevention effect of 1.0 compatibility is fresh without significant difference, i.e. tripterygium wilfordii on the whole
Leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:1.0 compatibility is pressed per 667m2It is its compatibility concentration that 15kg, which is applied,
The upper limit.Accounted in terms of cost and prevention effect 2, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:
1.5:0.75 compatibility, its is cost-effective, and raw material, usage amount are moderate, and prevention effect is again good, is preferable optimization formula ratio
With amount of application.The effect of processing 19-27 preventing and treating tomato root-knot eelworms is significantly better than CK3, and the effect for handling 22-24 is again aobvious on the whole
Write better than processing 19-21, with processing 25-27 on the whole without significant difference, i.e. tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the matter of leached tea oil slag
Amount is than being 2.5:1.5:0.75 compatibility is pressed per 667m2The prevention effect that 5-15kg is applied is significantly better than tripterygium wilfordii fresh leaf, polygonum flaccidum
Fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5 compatibility is pressed per 667m2The prevention effect that 5-15kg is applied, i.e. tripterygium wilfordii
Fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5 compatibility is pressed per 667m2It is its compatibility concentration that 5kg, which is applied,
Lower limit.Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:It is 0.75 compatibility and tripterygium wilfordii fresh leaf, peppery
Knotweed fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:Its prevention effect of 1.0 compatibility is fresh without significant difference, i.e. tripterygium wilfordii on the whole
Leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:1.0 compatibility is pressed per 667m2It is its compatibility concentration that 15kg, which is applied,
The upper limit.Accounted in terms of cost and prevention effect 2, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:
1.5:0.75 compatibility, its is cost-effective, and raw material, usage amount are moderate, and prevention effect is again good, is preferable optimization formula ratio
With amount of application.
Tomato during 4. ingredients test process 15d of table, the root knot number slip on wild cabbage（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 19 | 71.20±3.22d | Processing 28 | 79.12±0.93cd |
| Processing 20 | 75.40±0.59cd | Processing 29 | 75.69±2.88d |
| Processing 21 | 79.34±2.33c | Processing 30 | 83.92±1.04c |
| Processing 22 | 86.41±1.28b | Processing 31 | 87.71±2.81bc |
| Processing 23 | 92.26±0.57ab | Processing 32 | 90.35±3.30b |
| Processing 24 | 95.49±4.19a | Processing 33 | 94.98±0.76a |
| Processing 25 | 91.67±1.65ab | Processing 34 | 91.31±4.55ab |
| Processing 26 | 93.36±0.62ab | Processing 35 | 93.58±0.48ab |
| Processing 27 | 97.10±2.78a | Processing 36 | 96.50±1.81a |
| CK3 | 0.80±0.35e | CK4 | 0.02±0.01e |
The effect of processing 28-36 preventing and treating wild cabbage root-knot nematodes is significantly better than CK4, and the effect for handling 31-33 is again aobvious on the whole
Write better than processing 28-30, with processing 34-36 on the whole without significant difference, i.e. tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the matter of leached tea oil slag
Amount is than being 2.5:1.5:0.75 compatibility is pressed per 667m2The prevention effect that 5-15kg is applied is significantly better than tripterygium wilfordii fresh leaf, polygonum flaccidum
Fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5 compatibility is pressed per 667m2The prevention effect that 5-15kg is applied, i.e. tripterygium wilfordii
Fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.0:1.0:0.5 compatibility is pressed per 667m2It is its compatibility concentration that 5kg, which is applied,
Lower limit.Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:It is 0.75 compatibility and tripterygium wilfordii fresh leaf, peppery
Knotweed fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:Its prevention effect of 1.0 compatibility is fresh without significant difference, i.e. tripterygium wilfordii on the whole
Leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 3.0:2.0:1.0 compatibility is pressed per 667m2It is its compatibility concentration that 15kg, which is applied,
The upper limit.Accounted in terms of cost and prevention effect 2, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:
1.5:0.75 compatibility, its is cost-effective, and raw material, usage amount are moderate, and prevention effect is again good, is preferable optimization formula ratio
With amount of application.
More than to capsicum, cucumber, the experiment of four kinds of root knot nematodes of tomato and wild cabbage it was found from, it is tripterygium wilfordii fresh leaf, peppery
Knotweed fresh leaf, the mass ratio of leached tea oil slag are 2.0~3.0:1.0~2.0:0.5~1.0 presses per 667m25-15kg, which is applied, includes it
The upper limit and lower limit of prevention effect.Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 by every
667m210kg is applied, and its is cost-effective, and raw material, usage amount are moderate, and prevention effect is again good, is preferable optimization formula ratio
With amount of application.It is same to press per 667m it was found from table 1-2 and table 3-4 comparative analysis2The prevention effect of 5-15kg amounts of application, its
Table 3-4 prevention effect be significantly better than table 1-2 prevention effect, i.e. ingredients prevention effect be significantly better than single dose preventing and treating effect
Really；And the prevention effect of ingredients is not equal to the summation of the prevention effect of each single dose of composition ingredients, the prevention effect of ingredients is more than
The summation of the prevention effect of each single dose of ingredients, i.e. tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the compatibility of leached tea oil slag are constituted with collaboration work
With, that is, prevention effect after its compatibility there is synergistic effect.
Auxiliary agent is tested
Auxiliary agent experiment is mainly qualitative and quantitative and determines influence of the auxiliary agent to the prevention effect of whole ingredients.
Materials and methods
3.1.1 test material
Experiment vegetable species are capsicum, cucumber, four kinds of vegetables of tomato and wild cabbage.Experiment is tripterygium wilfordii fresh leaf with ingredients, peppery
Knotweed fresh leaf, auxiliary agent is leached tea oil slag.
Test method
Experiment is carried out in Yiyang proving ground.The plot that root-knot nematode is often sent out from former years is carried out.When capsicum, cucumber, kind
Prevention effect experiment is carried out when eggplant and the generation of the root-knot nematode of wild cabbage；Control is located at same plot.Test effect is mainly with same
When a kind of root knot nematode occurs, pour after dispenser agent, counted with the percentage of root knot quantity reduction and statistical analysis, compare
（CK）Poured and applied with water spot cave.Capsicum, cucumber, four kinds of vegetables of tomato and wild cabbage are selected cloudy or fine when root-knot nematode is broken out
It carries out dispenser processing.Each treatment agent is by 15 times of liquid（Refer to amount of water）The sprayer for spending shower nozzle is attacked a vital point to pour and applied.Pour administration
Do not water or apply fertilizer in 24h, observed and counted during 15d.Conventional rich water quality management after 24h.Experiment sets 12 processing 4
Individual control, each 3 repetitions of processing, each repeatedly 1.0m2, random distribution.Percentage is reduced using root knot quantity to imitate as processing
Really, in combination with field observation.The amount of application of each vegetables is shown in the following description to each processing.Processing 1：The experiment agent of capsicum
Amount ratio --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m210kg is applied；Place
Reason 2：The test dose ratio of capsicum --- tripterygium wilfordii fresh leaf, the mass ratio of polygonum flaccidum fresh leaf are 2.5:1.5 press per 667m210kg is applied
With；Processing 3：The test dose ratio of capsicum --- water, the mass ratio of leached tea oil slag are 10.0:1.0 press per 667m210kg is applied；
CK1：The control of capsicum is poured with water spot cave and applied.Processing 4：The test dose ratio of cucumber --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, tea seed
The mass ratio of the dregs of rice is 2.5:1.5:0.75 presses per 667m210kg is applied；Processing 5：Cucumber test dose ratio --- tripterygium wilfordii is fresh
Leaf, the mass ratio of polygonum flaccidum fresh leaf are 2.5:1.5 press per 667m210kg is applied；Processing 6：The test dose ratio of cucumber --- water,
The mass ratio of leached tea oil slag is 10.0:1.0 press per 667m210kg is applied；CK2：The control of cucumber is poured with water spot cave and applied.Processing 7：
The test dose ratio of tomato --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m2
10kg is applied；Processing 8：The test dose ratio of tomato --- tripterygium wilfordii fresh leaf, the mass ratio of polygonum flaccidum fresh leaf are 2.5:1.5 by every
667m210kg is applied；Processing 9：The test dose ratio of tomato --- water, the mass ratio of leached tea oil slag are 10.0:1.0 press per 667m2
10kg is applied；CK3：The control of tomato is poured with water spot cave and applied.Processing 10：It is the test dose ratio of wild cabbage --- tripterygium wilfordii fresh leaf, peppery
Knotweed fresh leaf, the mass ratio of leached tea oil slag are 2.5:1.5:0.75 presses per 667m210kg is applied；Processing 11：The test dose of wild cabbage
Mass ratio than --- tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf is 2.5:1.5 press per 667m210kg is applied；Processing 12：The examination of wild cabbage
Test dose ratio --- water, the mass ratio of leached tea oil slag are 10.0:1.0 press per 667m210kg is applied；CK4：The control water of wild cabbage
Attack a vital point to pour and apply.
Statistical analysis
SAS is used to test data（6.12）Carry out statistical analysis.As known from Table 5, processing 1 prevents and treats capsicum root-knot nematode
The mass ratio that effect is significantly better than CK1 and processing 2-3, i.e. tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag is 2.5:1.5:0.75 matches somebody with somebody
5 ingredients is pressed per 667m210kg pours the prevention effect applied preferably, is secondly that tripterygium wilfordii fresh leaf, the mass ratio of polygonum flaccidum fresh leaf are
2.5:The prevention effect of the ingredients of 1.5 compatibilities.Experiment shows that the prevention effect that compatibility has auxiliary agent is significantly better than the preventing and treating of no auxiliary agent
Effect.Thus illustrate, auxiliary agent leached tea oil slag is for root-knot nematode to have significant synergies on preventing and treating capsicum in this ingredients.
Capsicum during 5. auxiliary agent test process 15d of table, the root knot number slip on cucumber（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 1 | 95.26±0.97a | Processing 4 | 96.09±2.38a |
| Processing 2 | 73.49±2.64b | Processing 5 | 80.92±1.67b |
| Processing 3 | 8.89±0.30c | Processing 6 | 5.90±0.58c |
| CK1 | 1.69±0.09d | CK2 | 0.52±0.17d |
The effect of root-knot nematode is significantly better than CK2 on the preventing and treating cucumber of processing 4 and processing 5-6, i.e. tripterygium wilfordii fresh leaf, polygonum flaccidum are fresh
Leaf, the mass ratio of leached tea oil slag are 2.5:1.5:The ingredients of 0.75 compatibility is pressed per 667m210kg pours the prevention effect applied preferably, its
Secondary is that tripterygium wilfordii fresh leaf, the mass ratio of polygonum flaccidum fresh leaf are 2.5:The prevention effect of 1.5 compatibilities.Experiment shows, only auxiliary agent tea seed
The prevention effect of the dregs of rice handles 4-5 prevention effect far away from ingredients；The prevention effect of the processing 5 of ingredients without auxiliary agent again far away from
There is the prevention effect of the processing 4 of auxiliary agent ingredients.Thus illustrate, auxiliary agent leached tea oil slag is used in this ingredients root-knot nematode on preventing and treating cucumber
Have significant synergies.
As known from Table 6, the effect of the preventing and treating of processing 7 tomato root-knot eelworm is significantly better than CK3 and processing 8-9, the effect of processing 8
Be significantly better than again processing 9, i.e. tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag mass ratio be 2.5:1.5:The ingredients of 0.75 compatibility
By every 667m210kg pours the prevention effect applied preferably, is secondly that tripterygium wilfordii fresh leaf, the mass ratio of polygonum flaccidum fresh leaf are 3.5:1.5 match somebody with somebody
The prevention effect of 5 ingredients.Experiment shows that only the prevention effect of auxiliary agent leached tea oil slag handles 7-8 preventing and treating effect far away from ingredients
Really；The prevention effect of the processing 8 of ingredients without auxiliary agent is again far away from the prevention effect for the processing 7 for having auxiliary agent ingredients.Thus illustrate,
Auxiliary agent leached tea oil slag is used for preventing and treating tomato root-knot eelworm in this ingredients and had significant synergies.
Tomato during 6. auxiliary agent test process 15d of table, the root knot number slip on wild cabbage（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 7 | 92.26±3.19a | Processing 10 | 90.35±4.81a |
| Processing 8 | 84.16±0.82b | Processing 11 | 79.84±1.64b |
| Processing 9 | 8.44±1.41c | Processing 12 | 7.51±1.37c |
| CK3 | 0.80±0.34d | CK4 | 0.02±0.01d |
The effect that processing 10 prevents and treats wild cabbage root-knot nematode is significantly better than CK4 and processing 11-12, and the effect of processing 11 is again notable
Mass ratio better than processing 12, i.e. tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag is 2.5:1.5:The ingredients of 0.75 compatibility is by every
667m210kg pours the prevention effect applied preferably, is secondly that tripterygium wilfordii fresh leaf, the mass ratio of polygonum flaccidum fresh leaf are 3.5:1.5 compatibilities
The prevention effect of ingredients.Experiment shows that only the prevention effect of auxiliary agent leached tea oil slag handles 10-11 preventing and treating effect far away from ingredients
Really；The prevention effect of the processing 11 of ingredients without auxiliary agent is again far away from the prevention effect for the processing 10 for having auxiliary agent ingredients.Thus say
Bright, auxiliary agent leached tea oil slag is used for preventing and treating wild cabbage root-knot nematode in this ingredients and had significant synergies.
Experiment shows above（Table 5-6）, significantly, the effect of adding assistant is notable for the difference on effect of adding assistant and not adding assistant
Better than the effect of not adding assistant, its effect of adding assistant is not significantly reduced.Experiment also shows（Table 1-6）, organic vegetable root-knot nematode
Preventing and treating is all significantly higher than CK with tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag compatibility whether effect；Auxiliary agent leached tea oil slag, tripterygium wilfordii
Fresh leaf, the mass ratio of polygonum flaccidum fresh leaf are 0.75:2.5:The ingredients of 1.5 compatibilities is pressed per 667m210kg pours the prevention effect applied most
Good, its compatibility is optimal compatibility（Table 1-6）.Experiment shows, after 15d, its main functional component wilfordine, general flavone,
Tea Saponin etc. is not detected, does not during which descend moderate rain or heavy rain；After, during which following moderate rain or heavy rain more than 1 time, after 10d, it is led
Functional component wilfordine, general flavone, Tea Saponin etc. is wanted not to detect.Tripterygium wilfordii（Tripterygium wilfordii Hook.
f.）, Celastraceae, tripterygium plant.It is also known as herba fibraureae recisae, yellow cured rattan etc..Liana shrub, main product in China, Korea, Japan, in
State is distributed mainly on various regions and southwest on the south the Yangtze river basin, and main product is in Fujian, Zhejiang, Anhui, Henan and other places.It is born in the shade
In more wet hillside, mountain valley, small stream side bushes.The hillside in happiness more cool place, with the sand soil or yellow earth that slant acidity, soil layer are deep
Soil most preferably grows.Bitter, pungent, cool in nature, big poison.Thunder God Calamus liana shrub, high 1-3 meters, sprig brownish red is thin with 4-6 roots
Rib, by Mi Mao and fine and closely woven hole skin.Elliptic leaf shape, the ovum that falls are oval, rectangular oval or avette, long 4-7.5 centimetres, wide 3-4 lis
Rice, tip is suddenly sharp or short tapering, base portion wealthy wedge shape or circle, and there is a serration at edge, 4-7 pairs of lateral vein, up to leaf margin after it is slightly upper curved；Leaf
Handle is long 5-8 millimeters, close by rust hair.Circular cone cyme is narrower, long 5-7 centimetres, wide 3-4 centimetres, generally there is 3-5 branches.
The long round shape of samara, long 1-1.5 centimetres, 1-1.2 centimetres of diameter, seed buttress shaft shape, up to 10 millimeters.Tripterygium wilfordii bitter, pungent, property
It is cool, big poison, with clearing heat and detoxicating, dispelling wind and removing obstruction in the meridians, relax the muscles and stimulate the blood circulation, swelling and pain relieving, desinsection stop blooding the effects such as, return liver and kidney channel, work(
Effect dispelling wind and eliminating dampness, remove obstruction in channels to relieve pain, swelling and pain relieving, removing toxic substances desinsection.The traditional Chinese medical science is used to treat lepra reaction, rheumatoid arthritis, lung
Tuberculosis and other chronic lung diseases etc..Tripterygium wilfordii has the effect such as antitumor, anti-inflammatory, but its is very toxic, so tripterygium wilfordii is in doctor
Application in treatment is constantly subjected to limitation.Its main chemical of tripterygium wilfordii is：Root contains wilfordine（wilfordine）, tripterygium wilfordii
Secondary alkali（wilforine）, Wilforgine（wilforgine）, Wilfortrine is wilfortrine（Wil- skin acid amides
（celacinnine）, celastrus orbiculatus furancarboxylic acid amine（celafurine）, celastrus orbiculatus benzyl acid amides（celagbenzine）, Thunder God
Rattan lactone（wilforlide）A, B, triptonoterpenol（triptonoterpenol）, 16-hydroxytriptolide（16-
hydroxytripto-lide）, triptolide is triptolide（triptolide）, table Triptolide triol
（epitriptriolide）, thunder kaurene lactone（tripterifordine）, mapping-thunder kaurene lactone
（antriptolactone）, wilfordic acid（tripterygic acid）, straight wedge oxalic acid（orthosphenic acid）, β-paddy
Sterol（β-sitosterol）And Hu licopersicin（daucosterol）.Tripterygium wilfordii chemistry and Pharmacological Results show, its effectively into
Dividing has Diterpenes, triterpenes and alkaloid compound etc..Diterpene-kind compound in tripterygium wilfordii belongs to abietane-type, with α,
β-unsaturated lactone structure, pharmacological research confirms that it is the main component of tripterygium wilfordii bioactivity, mainly includes：Tripterygium wilfordii first
Element, triptolide, Triptolide triol, triptolide diol, Triptolide tetrol.Triterpenes in tripterygium wilfordii
Compound has：Wilforlide A, Wiofolide B, Orthosphenic acid A, Orthosphenic acid B, tripterygone, tripterygium wilfordii
Red pigment etc..Polygonum flaccidum（Polygonum flaccidum Meissn. ）It is polygonaceae Polygonum, alias：The burnt grass of class, flaccid knotweed herb etc., 1 year
Raw herbaceous plant, high 60~90cm, complete stool spreads gland point and young pilose antler.Stem is upright, or bottom is thrown oneself on the ground, and usual aubergine, section expands,
Leaf alternate, there is short handle.The wide lanceolar of blade, tip is tapering, base portion wedge shape, two sides by coarse wool, above bottle green, have splay
Blackspot, ochrea film quality, edge growth seta.Spike is born in Zhi Ding, and bennet is elongated, long 6~12cm, sagging, flower thinning；Perianth
5 drastic cracks, white spreads green gland point, top takes on a red color；Stamen 7~8；The Room of ovary 1,3 pieces of style.The rib of achene 3, outsourcing is harbored
Perianth.Summer at florescence.Florescence summer, aciculiform blade is to have splayed blackspot, and flower is born in Zhi Ding for spike sequence.It is national most of
Area is distributed, and is born in nearly waterside, dark and damp place.Polygonum flaccidum has an expelling wind and removing dampness, blood stasis removing analgesic, removing toxicity for detumescence, desinsection it is antipruritic it
Effect, the traditional Chinese medical science is used to treat dysentery, gastroenteritis, diarrhoea, rheumatic arthritis, treating swelling and pain by traumatic injury, functional uterine bleeding；Poison is controlled in external application
Snake bite, skin eczema etc..Herb contains protein, organic acid, carbohydrate, tannin, flavones monomer rutin (Rutin), Hypericum Chinense
Glycosides (Hyperin), quercetin (Quercetin), Isorhamnetin (Isorhamnetin) and kaempferol (Kaempferol) and wave
Hair oil n-decane, a small amount of nopinene, limonene and n-undecane etc., it is slightly poisonous.Oil tea（Camellia oleifera Abel.）
Belong to Theaceae, Camellia, alias：Ribes tree, tea oil tree etc..Evergreen dungarunga.Because its seed can extract oil（Tea oil）It is edible, therefore
Name.Shrub or middle arbor；Spray has coarse wool.Leaf keratin, ellipse, Long Circle or obovate, tip is sharp and has blunt nosed, sometimes gradually
It is sharp or blunt, base portion wedge shape, it is long 5-7 centimetres, it is wide 2-4 centimetres, it is sometimes longer, above bottle green, shinny, middle arteries have coarse wool or soft
Hair, below light green color, hairless or middle arteries become mildewed, and lateral vein can be shown in above, not have below it is obvious that there is serration at edge
When have cognate, petiole is long 4-8 millimeters, there is coarse wool.Tea oil tree is one of big woody oleiferous plants in the world four, and it is sub- that it is grown in southern china
The high mountain of torrid areas and hilly country, are the distinctive pure natural senior oil plants of one kind of China.China is from the Yangtze river basin to south China
Cultivate extensively various regions.It is concentrated mainly on Zhejiang, Jiangxi, Henan, Hunan, Guang Xiwu provinces.Tea oil color clear flavor incense, it is nutritious, it is resistance to
Storage, is high quality food oil；Also industry can be used for as lubricating oil, antirust oil.Tea cake is both agricultural chemicals, is fertilizer again, can be improved
Farmland water-holding capacity and preventing and treating rice pest.Containing Tea Saponin, tea seed polysaccharide, tea seed albumen etc. in leached tea oil slag, their all being
The raw material of work, light industry, food, feed industry product etc..
Experiment shows that main functional component wilfordine of tripterygium wilfordii fresh leaf etc. has good stomach poison function, and polygonum flaccidum is fresh
The functional components such as the general flavone of leaf+volatile oil n-decane not only have good action of contace poison, moreover it is possible to promote the work(such as wilfordine
The speed that energy composition is acted in root knot nematode toxicity in vivo, the two has collaboration, synergistic effect well.Tripterygium wilfordii is fresh
Leaf, the main functional component of polygonum flaccidum fresh leaf have to root knot nematode it is good tag, interior suction, the toxic action such as stomach toxicity.Tea
The functional components such as the Tea Saponin of the seed dregs of rice not only can effectively dissolve the functional components such as wilfordine, general flavone, significantly improve to vegetables
The toxic action of the tagging of root-knot nematode, interior suction, stomach toxicity etc., Tea Saponin etc. can also form soap-film, enhance tripterygium wilfordii fresh leaf, peppery
The toxic action of knotweed fresh leaf, is a kind of good auxiliary agent.
Embodiment
Implementation below and embodiment are intended to further illustrate the present invention, rather than limitation of the present invention.
Pour the preparation of root agent stoste：Material tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag all should be selected and come from soil, water, air
Etc. the free of contamination area of environmental condition, soil, water body and organic fertilizer etc. also want heavy metal free to pollute, tripterygium wilfordii, polygonum flaccidum, tea oil tree
It is that nature growth is formed, the mountain area of chemical fertilizer, chemical pesticide was not applied.Tripterygium leaf, polygonum flaccidum leaf use fresh and alive material, leached tea oil slag
For the dry cake of 1-30d moisture absorption of not going mouldy.First the soil on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, washing is not needed.With hammer
Leached tea oil slag is smashed to pieces, by leached tea oil slag:Water=1:10 ratio is soaked in water, after mixing after capping fermentation 15-20d, 15-20d,
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are soaked in 3-5d in leached tea oil slag zymotic fluid in the ratio of next segment description respectively.Tripterygium wilfordii is fresh
Leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy.Tripterygium wilfordii fresh leaf, polygonum flaccidum
Fresh leaf, the mass ratio of the component of leached tea oil slag three are 2.0~3.0:1.0~2.0:0.5~1.0, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are by fresh
Re-computation, leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, and leached tea oil slag is auxiliary agent.After capping immersion 3-5d, 4
Layer filtered through gauze 2~3 times, collects filtrate, is put into container, seals immediately, as mother liquor（Stoste）, can preserve 1-6 months.
When breaking out root-knot nematode, lid is opened, stoste is taken out, by every 667m215 times of liquid of 5~15kg stostes, 3 concentration gradients
（Refer to amount of water）The sprayer for spending shower nozzle is attacked a vital point to pour and applied.
The component tripterygium wilfordii fresh leaf of the above three, polygonum flaccidum fresh leaf, the most preferred mass ratio of leached tea oil slag are 2.5:1.5:0.75,
When breaking out root-knot nematode, lid is opened, stoste is taken out, by every 667m25~15kg stostes, 15 times of liquid（Refer to amount of water）Pour and apply.
Embodiment is implemented in Yiyang proving ground, and the plot that root-knot nematode is often sent out from former years is carried out.When capsicum, cucumber, tomato
Prevented and treated when breaking out root-knot nematode with wild cabbage；Control is located at same plot.Effect mainly breaks out root knot with same vegetables
During nematode, pour after dispenser agent, counted with the percentage of root knot quantity reduction and statistical analysis, compareed（CK）Poured and applied with water.It is peppery
Green pepper, cucumber, four kinds of vegetables of tomato and wild cabbage are when breaking out root-knot nematode, and selection carries out dispenser processing at cloudy day or fine day.Pour and apply
Do not water or apply fertilizer using in 24h, observed and counted during 15d.Conventional rich water quality management after 24h.
Embodiment 1：
Pour the preparation of root agent stoste：Material tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag all should be selected and come from soil, water, air
Etc. the free of contamination area of environmental condition, soil, water body and organic fertilizer etc. also want heavy metal free to pollute, tripterygium wilfordii, polygonum flaccidum, tea oil tree
It is that nature growth is formed, the mountain area of chemical fertilizer, chemical pesticide was not applied.Tripterygium leaf, polygonum flaccidum leaf use fresh and alive material, leached tea oil slag
For the dry cake of 1-30d moisture absorption of not going mouldy.First the soil on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, washing is not needed.With hammer
Leached tea oil slag is smashed to pieces, by leached tea oil slag:Water=1:10 ratio is soaked in water, after mixing after capping fermentation 15-20d, 15-20d,
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are soaked in 3-5d in leached tea oil slag zymotic fluid in the ratio of next segment description respectively.Tripterygium wilfordii is fresh
Leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy.Tripterygium wilfordii fresh leaf, polygonum flaccidum
Fresh leaf, the mass ratio of the component of leached tea oil slag three are 2.0:1.0:0.5, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is pressed
The dry cake weight of 1-30d moisture absorption of not going mouldy is calculated, and leached tea oil slag is auxiliary agent.After capping immersion 3-5d, 4 layers of filtered through gauze 2~3
It is secondary, filtrate is collected, container is put into, seals immediately, as mother liquor（Stoste）, can preserve 1-6 months.In outburst root-knot nematode
When, lid is opened, stoste is taken out, by every 667m215 times of liquid of 5kg stostes（Refer to amount of water）The sprayer for spending shower nozzle is attacked a vital point
Pour and apply.When breaking out root-knot nematode, lid is opened, stoste is taken out, by every 667m215 times of liquid of 5kg stostes（Refer to amount of water）Pour
Apply.Embodiment is implemented in Yiyang proving ground, is carried out from the plot of former years Chang Fagen border insect insect pest.When capsicum, Huang
Prevented and treated when melon, tomato and wild cabbage outburst root-knot nematode；Control is located at same plot.Effect is mainly quick-fried with same vegetables
When sending out root-knot nematode, pour after dispenser agent, counted with the percentage of root knot quantity reduction and statistical analysis, compare（CK）Poured with water
Apply.Capsicum, cucumber, four kinds of vegetables of tomato and wild cabbage are when breaking out root-knot nematode, and selection carries out dispenser processing at cloudy day or fine day.
Pour using not watering or applying fertilizer in 24h, observed and counted during 15d.Conventional rich water quality management after 24h.Tripterygium wilfordii is fresh
Leaf, polygonum flaccidum fresh leaf, the mass ratio of the component of leached tea oil slag three are 2.0:1.0:0.5 presses per 667m215 times of liquid of 5kg control agents stoste are poured
The prevention effect applied.When handling 15d, the percentage of root knot quantity reduction is：
The percentage of capsicum root knot quantity reduction is that 76.03%, CK is 1.69%, and prevention effect is notable；Cucumber root knot quantity
The percentage of reduction is that 73.24%, CK is 0.52%, and prevention effect is notable；The percentage of tomato root knot quantity reduction is 71.20%,
CK is 0.80%, and prevention effect is notable；The percentage of wild cabbage root knot quantity reduction is that 79.12%, CK is 0.02%, and prevention effect shows
Write.
Result of implementation shows that it is 2.0 that organic vegetable, which pours root agent tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag compatibility mass ratio,:
1.0:0.5 presses per 667m2It is notable that 15 times of liquid of 5kg control agents stoste pour the prevention effect applied.
Embodiment 2：
Pour the preparation of root agent stoste：Material tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag all should be selected and come from soil, water, air
Etc. the free of contamination area of environmental condition, soil, water body and organic fertilizer etc. also want heavy metal free to pollute, tripterygium wilfordii, polygonum flaccidum, tea oil tree
It is that nature growth is formed, the mountain area of chemical fertilizer, chemical pesticide was not applied.Tripterygium leaf, polygonum flaccidum leaf use fresh and alive material, leached tea oil slag
For the dry cake of 1-30d moisture absorption of not going mouldy.First the soil on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, washing is not needed.With hammer
Leached tea oil slag is smashed to pieces, by leached tea oil slag:Water=1:10 ratio is soaked in water, after mixing after capping fermentation 15-20d, 15-20d,
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are soaked in 3-5d in leached tea oil slag zymotic fluid in the ratio of next segment description respectively.Tripterygium wilfordii is fresh
Leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy.Tripterygium wilfordii fresh leaf, polygonum flaccidum
Fresh leaf, the mass ratio of the component of leached tea oil slag three are 2.5:1.5:0.75, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, leached tea oil slag
Calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, leached tea oil slag is auxiliary agent.After capping immersion 3-5d, 4 layers of filtered through gauze 2~3
It is secondary, filtrate is collected, container is put into, seals immediately, as mother liquor（Stoste）, can preserve 1-6 months.In outburst root-knot nematode
When, lid is opened, stoste is taken out, by every 667m215 times of liquid of 10kg stostes（Refer to amount of water）The sprayer for spending shower nozzle is attacked a vital point
Pour and apply.When breaking out root-knot nematode, lid is opened, stoste is taken out, by every 667m215 times of liquid of 10kg stostes（Refer to amount of water）
Pour and apply.Embodiment is implemented in Yiyang proving ground, is carried out from the plot of former years Chang Fagen border insect insect pest.When capsicum,
Prevented and treated when cucumber, tomato and wild cabbage outburst root-knot nematode；Control is located at same plot.Effect mainly uses same vegetables
When breaking out root-knot nematode, pour after dispenser agent, counted with the percentage of root knot quantity reduction and statistical analysis, compare（CK）Use water
Pour and apply.Capsicum, cucumber, four kinds of vegetables of tomato and wild cabbage are when breaking out root-knot nematode, and selection is carried out at dispenser at cloudy day or fine day
Reason.Pour using not watering or applying fertilizer in 24h, observed and counted during 15d.Conventional rich water quality management after 24h.Tripterygium wilfordii
Fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of the component of leached tea oil slag three are 2.5:1.5:0.75 presses per 667m215 times of 10kg control agents stoste
Liquid pours the prevention effect applied.When handling 15d, the percentage of root knot quantity reduction is：
The percentage of capsicum root knot quantity reduction is that 95.26%, CK is 1.69%, and prevention effect is notable；Cucumber root knot quantity
The percentage of reduction is that 96.09%, CK is 0.52%, and prevention effect is notable；The percentage of tomato root knot quantity reduction is 92.26%,
CK is 0.80%, and prevention effect is notable；The percentage of wild cabbage root knot quantity reduction is that 90.35%, CK is 0.02%, and prevention effect shows
Write.
Result of implementation shows that it is 2.5 that organic vegetable, which pours root agent tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag compatibility mass ratio,:
1.5:0.75 presses per 667m2It is notable that 15 times of liquid of 10kg control agents stoste pour the prevention effect applied.
Embodiment 3：
Pour the preparation of root agent stoste：Material tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag all should be selected and come from soil, water, air
Etc. the free of contamination area of environmental condition, soil, water body and organic fertilizer etc. also want heavy metal free to pollute, tripterygium wilfordii, polygonum flaccidum, tea oil tree
It is that nature growth is formed, the mountain area of chemical fertilizer, chemical pesticide was not applied.Tripterygium leaf, polygonum flaccidum leaf use fresh and alive material, leached tea oil slag
For the dry cake of 1-30d moisture absorption of not going mouldy.First the soil on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, washing is not needed.With hammer
Leached tea oil slag is smashed to pieces, by leached tea oil slag:Water=1:10 ratio is soaked in water, after mixing after capping fermentation 15-20d, 15-20d,
Tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are soaked in 3-5d in leached tea oil slag zymotic fluid in the ratio of next segment description respectively.Tripterygium wilfordii is fresh
Leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy.Tripterygium wilfordii fresh leaf, polygonum flaccidum
Fresh leaf, the mass ratio of the component of leached tea oil slag three are 3.0:2.0:1.0, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, and leached tea oil slag is pressed
The dry cake weight of 1-30d moisture absorption of not going mouldy is calculated, and leached tea oil slag is auxiliary agent.After capping immersion 3-5d, 4 layers of filtered through gauze 2~3
It is secondary, filtrate is collected, container is put into, seals immediately, as mother liquor（Stoste）, can preserve 1-6 months.In outburst root-knot nematode
When, lid is opened, stoste is taken out, by every 667m215 times of liquid of 15kg stostes（Refer to amount of water）The sprayer for spending shower nozzle is attacked a vital point
Pour and apply.When breaking out root-knot nematode, lid is opened, stoste is taken out, by every 667m215 times of liquid of 15kg stostes（Refer to amount of water）
Pour and apply.Embodiment is implemented in Yiyang proving ground, is carried out from the plot of former years Chang Fagen border insect insect pest.When capsicum,
Prevented and treated when cucumber, tomato and wild cabbage outburst root-knot nematode；Control is located at same plot.Effect mainly uses same vegetables
When breaking out root-knot nematode, pour after dispenser agent, counted with the percentage of root knot quantity reduction and statistical analysis, compare（CK）Use water
Pour and apply.Capsicum, cucumber, four kinds of vegetables of tomato and wild cabbage are when breaking out root-knot nematode, and selection is carried out at dispenser at cloudy day or fine day
Reason.Pour using not watering or applying fertilizer in 24h, observed and counted during 15d.Conventional rich water quality management after 24h.Tripterygium wilfordii
Fresh leaf, polygonum flaccidum fresh leaf, the mass ratio of the component of leached tea oil slag three are 3.0:2.0:1.0 press per 667m215 times of liquid of 15kg control agents stoste
Pour the prevention effect applied.When handling 15d, the percentage of root knot quantity reduction is：
The percentage of capsicum root knot quantity reduction is that 98.23%, CK is 1.69%, and prevention effect is notable；Cucumber root knot quantity
The percentage of reduction is that 97.41%, CK is 0.52%, and prevention effect is notable；The percentage of tomato root knot quantity reduction is 97.10%,
CK is 0.80%, and prevention effect is notable；The percentage of wild cabbage root knot quantity reduction is that 96.50%, CK is 0.02%, and prevention effect shows
Write.
Result of implementation shows that it is 3.0 that organic vegetable, which pours root agent tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, leached tea oil slag compatibility mass ratio,:
2.0:1.0 press per 667m2It is notable that 15 times of liquid of 15kg control agents stoste pour the prevention effect applied.
Claims (3)
1. a kind of organic vegetable pours root agent, it is characterised in that the organic vegetable pour root agent by tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf,
Auxiliary agent leached tea oil slag compatibility is brewed to be formed, and wherein the compatibility mass ratio of tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf and auxiliary agent leached tea oil slag is 2.0
~3.0:1.0~2.0:0.5~1.0, tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf are basis by fresh, leached tea oil slag by 1-30d suction of not going mouldy
Wet dry cake weight is calculated.
2. a kind of organic vegetable according to claim 1 pours root agent, it is characterised in that tripterygium wilfordii fresh leaf, polygonum flaccidum fresh leaf, tea
The preferred mass ratio of the seed dregs of rice is 2.5:1.5:0.75.
3. a kind of a kind of organic vegetable as claimed in claim 1 pours the application method of root agent, it is characterised in that leached tea oil slag needs elder generation
By leached tea oil slag:Water is 1:10 ratio, which adds water, is made leached tea oil slag zymotic fluid, is steeped with this zymotic fluid as brewed medium by compatibility ratio
Compatibility liquid stoste is made, before root knot nematode outburst or when breaking out, by every 667m2With 5~15kg stostes, 15 times of liquid to vegetable
Dish root knot pour and applies, and selection is carried out at cloudy day or fine day, using not watering or apply fertilizer in rear 24h, conventional fertilizer water pipe after 24h
Reason, if then needing to handle again after using moderate rain or heavy rain, rain has been descended within rear 24h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN1140019A (en) * | 1996-03-19 | 1997-01-15 | 周明星 | Method for preparing Chinese medicinal herbs insecticide |
| CN101288413A (en) * | 2008-05-26 | 2008-10-22 | 浙江省农业科学院 | Tripterygium wilfordii compound pesticides for high effectively preventing and curing tea geometrid |
| CN101695310A (en) * | 2009-10-19 | 2010-04-21 | 舒伟燐 | Environment-friendly pesticide |
| CN102018001A (en) * | 2009-09-14 | 2011-04-20 | 周新荣 | Plant medicament for preventing corn aphids |
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Patent Citations (4)
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|---|---|---|---|---|
| CN1140019A (en) * | 1996-03-19 | 1997-01-15 | 周明星 | Method for preparing Chinese medicinal herbs insecticide |
| CN101288413A (en) * | 2008-05-26 | 2008-10-22 | 浙江省农业科学院 | Tripterygium wilfordii compound pesticides for high effectively preventing and curing tea geometrid |
| CN102018001A (en) * | 2009-09-14 | 2011-04-20 | 周新荣 | Plant medicament for preventing corn aphids |
| CN101695310A (en) * | 2009-10-19 | 2010-04-21 | 舒伟燐 | Environment-friendly pesticide |
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| Title |
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| "中草药治虫";游德福;《老区建设》;19951125(第11期);第47页第2段 * |
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