CN104904757A - Organic vegetable root watering agent and using method thereof - Google Patents

Organic vegetable root watering agent and using method thereof Download PDF

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CN104904757A
CN104904757A CN201510208376.6A CN201510208376A CN104904757A CN 104904757 A CN104904757 A CN 104904757A CN 201510208376 A CN201510208376 A CN 201510208376A CN 104904757 A CN104904757 A CN 104904757A
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fresh leaf
tea oil
root
oil slag
leached tea
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CN104904757B (en
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杨洁
胡勇屏
郭兆武
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Changsha University of Science and Technology
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Changsha University of Science and Technology
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Abstract

The invention discloses an organic vegetable root watering agent and a using method thereof, the organic vegetable root watering agent is a compatible mixture prepared by compatibility of fresh leaves of thunder god vine, fresh leaves of red-knees herb, and tea seed meal, wherein the mass ratio of the fresh leaves of thunder god vine, the fresh leaves of red-knees herb and the tea seed meal is 2.0-3.0:1.0-2.0:0.5-1.0, and is preferably 2.5:1.5:0.75, the fresh leaves of thunder god vine and the fresh leaves of red-knees herb are weighed freshly, the tea seed meal is weighed according to the 1 to 30-day non-mildewed and non-moistened dry cake weight, the tea seed meal is first prepared into a tea seed meal fermented liquid by adding water in the proportion of tea seed meal to water of 1:10, the tea seed meal fermented liquid is used both as an assistant and an infusion medium, and the compatible infusion liquid is the concentrate liquid. This compatible agent has remarkable control effect on organic vegetable root knot nematode, and has synergic effect. Before and during the outbreak of the root knot nematodes in vegetables, 15 times of 5-15 kg per 667 m<2> of the concentrate liquid are applied by watering, the root knot nematodes can be quickly killed, the spread is prevented, the plant recovery is speeded up, and the organic vegetable root watering agent is easy to produce and use, simple to operate, low in cost, and remarkable in control effect.

Description

A kind of organic vegetable waters root agent and using method thereof
Technical field
The invention belongs to agriculture practical new technology research and development and application; The present invention relates to research and development and production application method that a kind of organic vegetable waters root agent.
Background technology
Root knot nematode { Meloidogynespp, Root-knot nematodes) be that the obligatory parasitism parasitized in root system of plant is biological, there is host range extensive, strong adaptability, the features such as fertility is strong, cause larger impact and harm to modern agricultural production and development.Along with the increase of China's growing vegetables area, the generation of root knot nematode disease increases the weight of year by year, after disease occurs, generally causes output to reduce 20%-30%, reaches more than 75%, even have no harvest time serious.According to related data statistics, the whole world is every year because root-knot nematode endangers the crops economic loss that causes about more than 1,000 hundred million dollars.Root-knot nematode disease has increased the weight of the generation of silborne fungal diseases simultaneously, as: fusarium wilt, root rot.Within 2011, find that various vegetables crop has all infected root-knot nematode at Song Bu town, Macheng City of Hubei Province Vegetable Base, cause serious economic loss.
Root knot nematode disease is one of normal disease having a strong impact on output occurred on vegetables.Beans, melon, vegetables such as capsicum class, solanaceous vegetables, vegetables with green leaves class, root vegetables and green onion garlic class are nearly all its hosts, production loss is seriously even had no harvest, and can increase the weight of again the generation of the various native transmissibility fungal disease such as fusarium wilt, root rot and part bacterial disease simultaneously.
At present, physical control, chemical control, biological control etc. are mainly contained to the prevention and controls of root knot nematode disease.Physical control is as carried out soil-fumigating in batch phase of having a rest, and chemical control is as used chemical agent A Weizhi line treasured etc., and biological control microbial inoculum as various in seed selection, screens disease-resistant variety etc.Physical control is time-consuming takes a lot of work, and biological control does not also select high-effect bacterial, though the chemical agent that chemical control is existing good, the production of organic vegetable can not use chemical agent, and organic vegetable is forbidden in producing to use, and uses because which limit it.This organic vegetable waters root agent and overcomes above defect, both pollution-free, and have again good control efficiency, nature taken from by raw material, wide material sources, and cost is low, is that a kind of well organic vegetable waters root agent.Disease uses before occurring, and can prevent to occur; Use after generation, comparatively fast can kill rhizosphere nematode, stop nematode to spread, plant restore normal growth soon.It is achievement through the application of R & D and manufacture for many years that this organic vegetable waters root agent, and it both can be prevented, can control again, and easy to use, simple to operate, and cost is low, and control efficiency is remarkable, and this is other about medicament or measure institute cannot be than.
Summary of the invention
Object of the present invention aims to provide a kind of organic vegetable and waters root agent, easy to use, simple to operate, and cost is low, and control efficiency is remarkable.Another object of the present invention aims to provide the preferably using method that above-mentioned organic vegetable waters root agent.The object of the invention is to realize in the following manner:
Control agent of the present invention is the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag composition, and wherein leached tea oil slag is mainly used as auxiliary agent, plays a part to promote its control efficiency, doubles as brewed medium.Inventor studies discovery, adds auxiliary agent leached tea oil slag and can play good synergistic effect.Need it is emphasized that, this organic vegetable waters the fresh leaf of thunder godvine of root agent, the fresh leaf of polygonum flaccidum, leached tea oil slag all should be selected from free of contamination areas of environmental condition such as soil, water, air, soil, water body and fertilizer etc. also want heavy metal free to pollute, thunder godvine, polygonum flaccidum, tea oil tree system self-sow form, and do not use the mountain area of chemical fertilizer, chemical pesticide.Otherwise, will the quality of organic vegetable be affected.The preparation method that this organic vegetable waters root agent is: material tripterygium leaf, polygonum flaccidum leaf, leached tea oil slag all should be selected from free of contamination areas of environmental condition such as soil, water, air, tripterygium leaf, polygonum flaccidum leaf all use fresh and alive material, and leached tea oil slag is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, do not need washing.Leached tea oil slag is smashed to pieces, in leached tea oil slag with hammer: the ratio of water=1:10 is soaked in water, after adding a cover fermentation 15-20d, 15-20d after mixing, fresh for thunder godvine leaf, the fresh leaf of polygonum flaccidum are put into and wherein soaked 3-5d.The fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag three components is 2.0 ~ 3.0:1.0 ~ 2.0:0.5 ~ 1.0, and the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.Smash leached tea oil slag to pieces: the ratio of water=1:10 is soaked in water, fermentation 15-20d is added a cover after mixing, after 15-20d, fresh for thunder godvine leaf, the fresh leaf of polygonum flaccidum are put into and wherein soaked 3-5d, after 3-5d, 4 layers of filtered through gauze 2 ~ 3 times, collect filtrate, put into container, seal immediately, be this organic vegetable and water root agent stoste (i.e. mother liquor), 1-6 month can be preserved.This organic vegetable waters root agent and uses before disease occurs, and prevention is by every 667m 25 ~ 10kg stoste, 15 times of liquid water and execute (sprayer spending shower nozzle is attacked a vital point and watered Shi Gengjia); Use, by every 667m after insect pest occurs 210 ~ 15kg stoste, 15 times of liquid water and execute (sprayer spending shower nozzle is attacked a vital point and watered Shi Gengjia), can comparatively fast kill rhizosphere insect, stop insect pest to spread.This control agent is produced upper easy to use, simple to operate, and cost is low, and control efficiency is remarkable, and the shelf-life is longer, packs with plastic bag sealing stoste (i.e. mother liquor), can carry out factorial praluction, forms product.
The most preferred mass ratio of the fresh leaf of above three components thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is 2.5:1.5:0.75, and the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.The present invention selects the fresh leaf of control agent thunder godvine, the fresh leaf of polygonum flaccidum and auxiliary agent leached tea oil slag, and have employed the compatibility of suitable concentration, be mixed with commercial control agent, it both can be prevented, can control again, and it has significant preventive and therapeutic effect to organic vegetable root-knot nematode.Using this organic vegetable, to water root agent be its comparatively one of effective measures.This control agent is easy to use, simple to operate, and cost is low, and control efficiency is remarkable, and this is other about medicament or measure institute cannot be than.It is the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum and auxiliary agent leached tea oil slag that this organic vegetable waters root agent prescription, and brewed by the ratio mixing of above-mentioned mass ratio, mother liquor plastic bag sealing is packed, and shady and cool dry lucifuge place preserves, 6 months shelf-lifves.The fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.It is below the comparative test of its regulating effect.Organic vegetable of the present invention waters the report that root agent has no like product at present, and the present invention is the result of the test that inventor draws through experimental study for many years and production practices, and is verified in the production of long period, and effect is better.And formula of the present invention also possesses skills, content is higher, and formulation is more stable, abundant raw material source, easy and simple to handle, as being developed to commodity, and low price.Easy to use aborning, control efficiency is remarkable, and use cost is low, is that a kind of good organic vegetable waters root agent.Organic vegetable waters root agent prescription can carry out factorial praluction, and its exploitation not only commercially will increase a kind of novel organic vegetable and water root agent, also provide an approach solved by the problem for current organic vegetable root knot nematode control poor effect.Using method of the present invention is: this organic vegetable waters root agent and uses before root-knot nematode occurs, and prevention is by every 667m 25 ~ 10kg stoste, 15 times of liquid water to be executed; Use, by every 667m after root-knot nematode occurs 210 ~ 15kg stoste, 15 times of liquid water to be executed, and comparatively fast can kill root-knot nematode, stops nematode to spread.
Use in rear 24h and can not sprinkle water or apply fertilizer, conventional fertilizer water management after 24h, has descended moderate rain or heavy rain if used within rear 24h, has then needed again to process after rain.Test shows, after using 15d, detects vegetables, and the main functional component of its medicament does not detect, and period does not descend moderate rain or heavy rain; After using, period following moderate rain or heavy rain more than 1 time, after using 7d, its main functional component does not detect.
Be below invent artificially to realize object of the present invention, constantly selected by test and select applicable organic vegetable of the present invention to water the comparative test of the control efficiency that the raw material of root agent prescription and relevant proportioning thereof are done.
one pack system is tested
One pack system tests the single effect test that each component of mainly qualitative determination is prevented and treated organic vegetable root-knot nematode.
materials and methods
1.1.1 test material
Test vegetable species select the most easily break out root-knot nematode capsicum, cucumber, tomato and wild cabbage four kinds of vegetables.Test ingredients is: the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag.
test method
The preparation of test material: the fresh leaf of material thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag, all should select from free of contamination areas of environmental condition such as soil, water, air, soil, water body and fertilizer etc. also want heavy metal free to pollute, thunder godvine, polygonum flaccidum, tea oil tree system self-sow form, and do not use the mountain area of chemical fertilizer, chemical pesticide.Tripterygium leaf, polygonum flaccidum leaf all use fresh and alive material, and leached tea oil slag is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, do not need washing.With hammer, leached tea oil slag is smashed to pieces, in leached tea oil slag: the ratio of water=1:10 is soaked in water, after adding a cover fermentation 15-20d, 15-20d after mixing, the ratio that fresh for thunder godvine leaf, the fresh leaf of polygonum flaccidum describe in next section is respectively soaked in and is wherein immersed in 3-5d in leached tea oil slag zymotic fluid.The fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.Thunder godvine fresh leaf component is by following mass ratio preparation mother liquor: the fresh leaf of thunder godvine: leached tea oil slag zymotic fluid=2.5:5.0(wherein leached tea oil slag zymotic fluid is brewed medium, and its quality is included into its mother liquor gross mass).Polygonum flaccidum fresh leaf component is by following mass ratio preparation mother liquor, and the fresh leaf of polygonum flaccidum :=1.5:5.0(wherein leached tea oil slag zymotic fluid is brewed medium, and its quality is included into its mother liquor gross mass).Leached tea oil slag component prepares mother liquor by following mass ratio, leached tea oil slag: water=1.0:10.0(wherein water is brewed medium, and its quality is included into its mother liquor gross mass).The fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.Add a cover and soak after 3-5d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be female stoste of each component single dose, can preserve 1-6 month.When breaking out root-knot nematode, lid being opened, taking out each single dose stoste (component is taken out and deposited 30d's) respectively, by every 667m 25,10 the sprayer that, 15 times of liquid (referring to amount of water) of 15kg stoste 3 concentration gradients spend shower nozzle is attacked a vital point to water and is executed.
Test is carried out in proving ground, Yiyang.The plot often sending out root-knot nematode former years is selected to carry out.Control efficiency test is carried out when capsicum, cucumber, tomato and wild cabbage root-knot nematode occur; Contrast is located at same plot.When test effect mainly occurs slow with the new root of same vegetable seedling, after watering dispenser agent, count and statistical analysis with the percentage that root knot quantity reduces, contrast (CK) is watered with water spot cave and is executed.Capsicum, cucumber and tomato three kinds of vegetables, when root-knot nematode occurs, are selected to process at cloudy day or fine day.Each single dose amount of application: its mother liquor amount of application of thunder godvine fresh leaf component is by every 667m 25kg, 10kg, 15kg tri-kinds of concentration gradients carry out watering to execute using.Its mother liquor amount of application of polygonum flaccidum fresh leaf component is by every 667m 25kg, 10kg, 15kg tri-kinds of concentration gradients carry out watering to execute using.Its mother liquor amount of application of leached tea oil slag component is by every 667m 25kg, 10kg, 15kg tri-kinds of concentration gradients carry out watering to execute using.Water to execute in process 24h and do not water or apply fertilizer, carry out during 15d observing and count.Conventional fertilizer water management after 24h.Test arranges 36 process, 4 contrasts, each process 3 repetition, each repetition 1.0m 2, random distribution.Using Percent mortality as treatment effect, simultaneously in conjunction with field observation.The disposition of each vegetables is shown in the following description to each process.
Process 1: the test dose of capsicum---the fresh leaf of thunder godvine is by every 667m 25kg uses; Process 2: the test dose of capsicum---the fresh leaf of thunder godvine is by every 667m 210kg uses; Process 3: the test dose of capsicum---the fresh leaf of thunder godvine is by every 667m 215kg uses; Process 4: the test dose of capsicum---the fresh leaf of polygonum flaccidum is by every 667m 25kg uses; Process 5: the test dose of capsicum---the fresh leaf of polygonum flaccidum is by every 667m 210kg uses; Process 6: the test dose of capsicum---the fresh leaf of polygonum flaccidum is by every 667m 215kg uses; Process 7: the test dose of capsicum---leached tea oil slag is by every 667m 25kg uses; Process 8: the test dose of capsicum---leached tea oil slag is by every 667m 210kg uses; Process 9: the test dose of capsicum---leached tea oil slag is by every 667m 215kg uses; CK1: the contrast of capsicum, waters with water spot cave and executes.Process 10: the test dose of cucumber---the fresh leaf of thunder godvine is by every 667m 25kg uses; Process 11: the test dose of cucumber---the fresh leaf of thunder godvine is by every 667m 210kg uses; Process 12: the test dose of cucumber---the fresh leaf of thunder godvine is by every 667m 215kg uses; Process 13: the test dose of cucumber---the fresh leaf of polygonum flaccidum is by every 667m 25kg uses; Process 14: the test dose of cucumber---the fresh leaf of polygonum flaccidum is by every 667m 210kg uses; Process 15: the test dose of cucumber---the fresh leaf of polygonum flaccidum is by every 667m 215kg uses; Process 16: the test dose of cucumber---leached tea oil slag is by every 667m 25kg uses; Process 17: the test dose of cucumber---leached tea oil slag is by every 667m 210kg uses; Process 18: the test dose of cucumber---leached tea oil slag is by every 667m 215kg uses; CK2: the contrast of cucumber, waters with water spot cave and executes.Process 19: the test dose of tomato---the fresh leaf of thunder godvine is by every 667m 25kg uses; Process 20: the test dose of tomato---the fresh leaf of thunder godvine is by every 667m 210kg uses; Process 21: the test dose of tomato---the fresh leaf of thunder godvine is by every 667m 215kg uses; Process 22: the test dose of tomato---the fresh leaf of polygonum flaccidum is by every 667m 25kg uses; Process 23: the test dose of tomato---the fresh leaf of polygonum flaccidum is by every 667m 210kg uses; Process 24: the test dose of tomato---the fresh leaf of polygonum flaccidum is by every 667m 215kg uses; Process 25: the test dose of tomato---leached tea oil slag is by every 667m 25kg uses; Process 26: the test dose of tomato---leached tea oil slag is by every 667m 210kg uses; Process 27: the test dose of tomato---leached tea oil slag is by every 667m 215kg uses; CK3: the contrast of tomato, waters with water spot cave and executes.Process 28: the test dose of wild cabbage---the fresh leaf of thunder godvine is by every 667m 25kg uses; Process 29: the test dose of wild cabbage---the fresh leaf of thunder godvine is by every 667m 210kg uses; Process 30: the test dose of wild cabbage---the fresh leaf of thunder godvine is by every 667m 215kg uses; Process 31: the test dose of wild cabbage---the fresh leaf of polygonum flaccidum is by every 667m 25kg uses; Process 32: the test dose of wild cabbage---the fresh leaf of polygonum flaccidum is by every 667m 210kg uses; Process 33: the test dose of wild cabbage---the fresh leaf of polygonum flaccidum is by every 667m 215kg uses; Process 34: the test dose of wild cabbage---leached tea oil slag is by every 667m 25kg uses; Process 35: the test dose of wild cabbage---leached tea oil slag is by every 667m 210kg uses; Process 36: the test dose of wild cabbage---leached tea oil slag is by every 667m 215kg uses; CK4: the contrast of wild cabbage, waters with water spot cave and executes.
statistical analysis
SAS(6.12 is adopted to test data) carry out statistical analysis.During process 15d, the effect of each process is in Table 1-2.Result of the test shows (table 1), and the root knot number slip of process 3 is significantly higher than process 1, illustrates that the Be very effective of higher concentration thunder godvine fresh leaf control capsicum root-knot nematode is better than the fresh leaf of thunder godvine of low concentration.Process 1-3 is significantly higher than again other process and CK1 on the whole, and namely the Be very effective of thunder godvine fresh leaf control capsicum root-knot nematode is better than the control efficiency of the fresh leaf of polygonum flaccidum, leached tea oil slag.The effect of variable concentrations polygonum flaccidum fresh leaf control capsicum root-knot nematode is: the root knot number slip of process 5-6 is significantly higher than process 4, illustrates that the Be very effective of higher concentration polygonum flaccidum fresh leaf control capsicum root-knot nematode is better than the fresh leaf of polygonum flaccidum of low concentration.Process 4-6 is significantly higher than again process 7-12 and CK1, and namely the Be very effective of polygonum flaccidum fresh leaf control capsicum root-knot nematode is better than leached tea oil slag control efficiency, but the control efficiency of the too late fresh leaf of thunder godvine.The effect of variable concentrations leached tea oil slag control capsicum root-knot nematode is: the process 9 of higher concentration and the process 7-8 root knot number slip of low concentration are without significant difference, but process 7-9 is significantly higher than CK1, namely leached tea oil slag has the effect of certain control to capsicum root-knot nematode, but the control efficiency of the too late fresh leaf of thunder godvine and the fresh leaf of polygonum flaccidum.
root knot number slip (unit: %) during table 1. one pack system test process 15d on capsicum, cucumber
Process 24h Process 24h
Process 1 30.20±3.40c Process 10 28.23±2.75b
Process 2 40.87±2.03ab Process 11 33.21±2.15ab
Process 3 49.79±1.76a Process 12 36.90±1.90a
Process 4 25.11±0.86d Process 13 21.30±3.64c
Process 5 38.94±3.52b Process 14 34.09±2.22ab
Process 6 32.67±1.70bc Process 15 38.35±2.81a
Process 7 6.20±3.07e Process 16 3.49±1.85d
Process 8 8.89±1.54e Process 17 5.90±0.90d
Process 9 9.21±0.56e Process 18 7.32±2.01d
CK1 1.69±0.87f CK2 0.52±0.28e
Note: lowercase is 0.05 level, the different letter representation significant difference of same row, lower same.
The effect of variable concentrations thunder godvine fresh leaf control cucumber root-knot nematode is: the root knot number slip of process 12 is significantly higher than process 10, illustrates that the Be very effective of higher concentration thunder godvine fresh leaf control cucumber root-knot nematode is better than the fresh leaf of thunder godvine of low concentration.Process 10-12 is significantly higher than again process 16-18 and CK2, and namely the Be very effective of thunder godvine fresh leaf control cucumber root-knot nematode is better than the control efficiency of leached tea oil slag.The effect of variable concentrations polygonum flaccidum fresh leaf control cucumber root-knot nematode is: the root knot number slip of process 15 is significantly higher than process 13, illustrates that the Be very effective of higher concentration polygonum flaccidum fresh leaf control cucumber root-knot nematode is better than the fresh leaf of polygonum flaccidum of low concentration.Process 13-15 is significantly higher than process 16-18 and CK2, and namely the Be very effective of polygonum flaccidum fresh leaf control capsicum root-knot nematode is better than the control efficiency of leached tea oil slag.The effect of variable concentrations leached tea oil slag control cucumber root-knot nematode is: the root knot number slip of process 16-18 without significant difference, but with CK2 significant difference.Namely leached tea oil slag control capsicum root-knot nematode has certain effect, but the control efficiency of the too late fresh leaf of thunder godvine and the fresh leaf of polygonum flaccidum.
Result of the test shows (table 2), and the root knot number slip of process 27 is significantly higher than process 25-26, illustrates that the Be very effective of higher concentration thunder godvine fresh leaf control tomato root-knot eelworm is better than the fresh leaf of thunder godvine of low concentration.Process 25-27 is significantly higher than again other process and CK3, namely on thunder godvine fresh leaf control tomato the Be very effective of rhizosphere insect be better than the fresh leaf of polygonum flaccidum, leached tea oil slag, control efficiency.
The effect of variable concentrations polygonum flaccidum fresh leaf control tomato root-knot eelworm is: the root knot number slip of the process 24 of higher concentration is significantly higher than the process 22 of low concentration.But the root knot number slip of process 22-24 is significantly higher than process 25-27 and CK3, namely the Be very effective of polygonum flaccidum fresh leaf control tomato root-knot eelworm is better than the control efficiency of leached tea oil slag.The effect of variable concentrations leached tea oil slag control tomato root-knot eelworm is: the process 27 of higher concentration and the process 25-26 root knot number slip of low concentration are without significant difference, but process 25-27 is significantly higher than CK3, the i.e. Be very effective of leached tea oil slag control tomato root-knot eelworm, but the control efficiency of the too late fresh leaf of thunder godvine and the fresh leaf of polygonum flaccidum.
root knot number slip (unit: %) during table 2. one pack system test process 15d on tomato, wild cabbage
Process 24h Process 24h
Process 19 26.76±2.23b Process 28 25.78±0.95b
Process 20 30.81±0.67ab Process 29 31.27±1.34a
Process 21 35.60±3.97a Process 30 32.38±1.66a
Process 22 20.19±2.55c Process 31 18.26±5.35c
Process 23 27.80±3.10b Process 32 21.45±3.21bc
Process 24 31.65±1.51ab Process 33 29.03±0.82a
Process 25 6.91±0.80d Process 34 5.33±1.54d
Process 26 8.44±1.69d Process 35 7.51±0.69d
Process 27 9.07±2.92d Process 36 9.35±2.13d
CK3 0.80±0.52e CK4 0.02±0.01e
On variable concentrations thunder godvine fresh leaf control wild cabbage, the effect of root-knot nematode is: the root knot number slip of process 29-30 is significantly higher than process 28, illustrates that the Be very effective of root-knot nematode on higher concentration thunder godvine fresh leaf control wild cabbage is better than the fresh leaf of thunder godvine of low concentration.Process 29-30 is significantly higher than again process 34-36 and CK4, and namely on thunder godvine fresh leaf control wild cabbage, the Be very effective of root-knot nematode is better than the control efficiency of leached tea oil slag.On variable concentrations polygonum flaccidum fresh leaf control wild cabbage, the effect of root-knot nematode is: the root knot number slip of process 33 is significantly higher than process 31-32, illustrates that the Be very effective of root-knot nematode on higher concentration polygonum flaccidum fresh leaf control wild cabbage is better than the fresh leaf of polygonum flaccidum of low concentration.Process 31-33 is significantly higher than process 34-36 and CK4, and namely on polygonum flaccidum fresh leaf control wild cabbage, the Be very effective of root-knot nematode is better than the control efficiency of leached tea oil slag.On variable concentrations leached tea oil slag control wild cabbage, the effect of root-knot nematode is: the root knot number slip of process 34-36 is significantly higher than CK4, but the control efficiency of the too late fresh leaf of thunder godvine and the fresh leaf of polygonum flaccidum.
Shown by above one pack system test, its effect of root-knot nematode on higher concentration thunder godvine fresh leaf control capsicum, cucumber, tomato, wild cabbage can reach remarkable result.The effect of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum is better, is secondly leached tea oil slag.Along with the reduction of concentration, control efficiency significantly declines.One pack system test also shows, each component is used alone, and its control efficiency is all lower than 50%.
compatibility mechanism
Compatibility mechanism mainly qualitative and quantitative measures the effect test that each ingredients prevents and treats organic vegetable rhizosphere insect.
materials and methods
2.1.1 test material
Test vegetable species select the most easily break out root-knot nematode capsicum, cucumber, tomato and wild cabbage four kinds of vegetables.Test ingredients is: the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag.
test method
The preparation of test material: the fresh leaf of material thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag all should be selected from free of contamination areas of environmental condition such as soil, water, air, soil, water body and fertilizer etc. also want heavy metal free to pollute, thunder godvine, polygonum flaccidum, tea oil tree system self-sow form, and do not use the mountain area of chemical fertilizer, chemical pesticide.Tripterygium leaf, polygonum flaccidum leaf all use fresh and alive material, and leached tea oil slag is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, do not need washing.Leached tea oil slag is smashed to pieces, in leached tea oil slag with hammer: the ratio of water=1:10 is soaked in water, after adding a cover fermentation 15-20d, 15-20d after mixing, the ratio that fresh for thunder godvine leaf, the fresh leaf of polygonum flaccidum describe in next section is respectively soaked in 3-5d in leached tea oil slag zymotic fluid.The fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag three components is 2.0 ~ 3.0:1.0 ~ 2.0:0.5 ~ 1.0, and the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and leached tea oil slag is auxiliary agent.Add a cover and soak after 3-5d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be mother liquor (stoste), can preserve 1-6 month.When breaking out root-knot nematode, lid being opened, taking out stoste, by every 667m 25,10 the sprayer that, 15 times of liquid (referring to amount of water) of 15kg stoste 3 concentration gradients spend shower nozzle is attacked a vital point to water and is executed.Test is carried out in proving ground, Yiyang.The plot often sending out root-knot nematode former years is selected to carry out.Control efficiency test is carried out when capsicum, cucumber, tomato and wild cabbage root-knot nematode occur; Contrast is located at same plot.When test effect mainly occurs with same root knot nematode, after watering dispenser agent, count and statistical analysis with the percentage that root knot quantity reduces, contrast (CK) is watered with water spot cave and is executed.
Capsicum, cucumber, tomato and wild cabbage four kinds of vegetables, when root-knot nematode is broken out, are selected to carry out dispenser process at cloudy day or fine day.Water to execute in process 24h and do not water or apply fertilizer, carry out during 15d observing and count.Conventional fertilizer water management after 24h.Test arranges 36 process, 4 contrasts, each process 3 repetition, each repetition 1.0m 2, random distribution.Percentage is reduced as treatment effect, simultaneously in conjunction with field observation using root knot quantity.The disposition of each vegetables is shown in the following description to each process.Process 1: the mass ratio of the test dose of capsicum---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 25kg uses; Process 2: the mass ratio of the test dose of capsicum---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 210kg uses; Process 3: the mass ratio of the test dose of capsicum---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 215kg uses; Process 4: the mass ratio of the test dose of capsicum---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 25kg uses; Process 5: the mass ratio of the test dose of capsicum---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 210kg uses; Process 6: the mass ratio of the test dose of capsicum---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 215kg uses; Process 7: the mass ratio of the test dose of capsicum---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 25kg uses; Process 8: the mass ratio of the test dose of capsicum---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 210kg uses; Process 9: the mass ratio of the test dose of capsicum---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 215kg uses; CK1: the contrast water spot cave of capsicum is watered and executed.Process 10: the mass ratio of the test dose of cucumber---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 25kg uses; Process 11: the mass ratio of the test dose of cucumber---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 210kg uses; Process 12: the mass ratio of the test dose of cucumber---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 215kg uses; Process 13: the mass ratio of the test dose of cucumber---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 25kg uses; Process 14: the mass ratio of the test dose of cucumber---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 210kg uses; Process 15: the mass ratio of the test dose of cucumber---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 215kg uses; Process 16: the mass ratio of the test dose of cucumber---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 25kg uses; Process 17: the mass ratio of the test dose of cucumber---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 210kg uses; Process 18: the mass ratio of the test dose of cucumber---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 215kg uses; CK2: the contrast water spot cave of cucumber is watered and executed.Process 19: the mass ratio of the test dose of tomato---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 25kg uses; Process 20: the mass ratio of the test dose of tomato---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 210kg uses; Process 21: the mass ratio of the test dose of tomato---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 215kg uses; Process 22: the mass ratio of the test dose of tomato---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 25kg uses; Process 23: the mass ratio of the test dose of tomato---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 210kg uses; Process 24: the mass ratio of the test dose of tomato---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 215kg uses; Process 25: the mass ratio of the test dose of tomato---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 25kg uses; Process 26: the mass ratio of the test dose of tomato---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 210kg uses; Process 27: the mass ratio of the test dose of tomato---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 215kg uses; CK3: the contrast water spot cave of tomato is watered and executed.Process 28: the mass ratio of the test dose of wild cabbage---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 25kg uses; Process 29: the mass ratio of the test dose of wild cabbage---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 210kg uses; Process 30: the mass ratio of the test dose of wild cabbage---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0:1.0:0.5 is by every 667m 215kg uses; Process 31: the mass ratio of the test dose of wild cabbage---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 25kg uses; Process 32: the mass ratio of the test dose of wild cabbage---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 210kg uses; Process 33: the mass ratio of the test dose of wild cabbage---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 215kg uses; Process 34: the mass ratio of the test dose of wild cabbage---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 25kg uses; Process 35: the mass ratio of the test dose of wild cabbage---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 210kg uses; Process 36: the mass ratio of the test dose of wild cabbage---the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 3.0:2.0:1.0 is by every 667m 215kg uses; CK4: the contrast water spot cave of wild cabbage is watered and executed.
statistical analysis
SAS(6.12 is adopted to test data) carry out statistical analysis.From table 3-4, the Be very effective that process 1-9 prevents and treats root-knot nematode is better than CK1, the effect of process 4-6 is significantly better than again processing 1-3 on the whole, and with process 7-9 on the whole without significant difference, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 2.5:1.5:0.75 is by every 667m 2the control efficiency that 5-15kg uses significantly be better than the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag mass ratio be that the compatibility of 2.0:1.0:0.5 is by every 667m 2the control efficiency that 5-15kg uses, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 2.0:1.0:0.5 is by every 667m 2it is the lower limit of its compatibility concentration that 5kg uses.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is the compatibility of 2.5:1.5:0.75 and the fresh leaf of thunder godvine, the mass ratio of the fresh leaf of polygonum flaccidum, leached tea oil slag be its control efficiency of compatibility of 3.0:2.0:1.0 on the whole without significant difference, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 3.0:2.0:1.0 is by every 667m 2it is the upper limit of its compatibility concentration that 15kg uses.Consider from cost and control efficiency 2 aspects, the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is the compatibility of 2.5:1.5:0.75, and it is cost-saving, and raw material, usage amount are all moderate, and control efficiency is good again, be good optimization formula ratio and amount of application.
root knot number slip (unit: %) during table 3. ingredients test process 15d on capsicum, cucumber
Process 24h Process 24h
Process 1 76.03±2.30c Process 10 73.24±3.37c
Process 2 81.87±3.18bc Process 11 79.51±3.31bc
Process 3 84.79±0.83b Process 12 83.90±1.58b
Process 4 86.27±3.09b Process 13 85.55±2.91b
Process 5 95.26±1.42ab Process 14 96.09±3.45a
Process 6 97.21±4.10a Process 15 95.62±2.58a
Process 7 94.35±1.82ab Process 16 91.22±0.70ab
Process 8 95.80±0.64ab Process 17 95.05±3.11a
Process 9 98.23±3.25a Process 18 97.41±1.93a
CK1 1.69±0.27d CK2 0.52±0.14d
The Be very effective that process 10-18 prevents and treats cucumber root-knot nematode is better than CK2, the effect of process 13-15 is significantly better than again processing 10-12 on the whole, with process 16-18 on the whole without significant difference, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 2.5:1.5:0.75 is by every 667m 2the control efficiency that 5-15kg uses significantly be better than the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag mass ratio be that the compatibility of 2.0:1.0:0.5 is by every 667m 2the control efficiency that 5-15kg uses, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 2.0:1.0:0.5 is by every 667m 2it is the lower limit of its compatibility concentration that 5kg uses.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is the compatibility of 2.5:1.5:0.75 and the fresh leaf of thunder godvine, the mass ratio of the fresh leaf of polygonum flaccidum, leached tea oil slag be its control efficiency of compatibility of 3.0:2.0:1.0 on the whole without significant difference, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 3.0:2.0:1.0 is by every 667m 2it is the upper limit of its compatibility concentration that 15kg uses.Consider from cost and control efficiency 2 aspects, the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is the compatibility of 2.5:1.5:0.75, and it is cost-saving, and raw material, usage amount are all moderate, and control efficiency is good again, be good optimization formula ratio and amount of application.The Be very effective that process 19-27 prevents and treats tomato root-knot eelworm is better than CK3, the effect of process 22-24 is significantly better than again processing 19-21 on the whole, with process 25-27 on the whole without significant difference, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 2.5:1.5:0.75 is by every 667m 2the control efficiency that 5-15kg uses significantly be better than the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag mass ratio be that the compatibility of 2.0:1.0:0.5 is by every 667m 2the control efficiency that 5-15kg uses, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 2.0:1.0:0.5 is by every 667m 2it is the lower limit of its compatibility concentration that 5kg uses.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is the compatibility of 2.5:1.5:0.75 and the fresh leaf of thunder godvine, the mass ratio of the fresh leaf of polygonum flaccidum, leached tea oil slag be its control efficiency of compatibility of 3.0:2.0:1.0 on the whole without significant difference, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 3.0:2.0:1.0 is by every 667m 2it is the upper limit of its compatibility concentration that 15kg uses.Consider from cost and control efficiency 2 aspects, the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is the compatibility of 2.5:1.5:0.75, and it is cost-saving, and raw material, usage amount are all moderate, and control efficiency is good again, be good optimization formula ratio and amount of application.
root knot number slip (unit: %) during table 4. ingredients test process 15d on tomato, wild cabbage
Process 24h Process 24h
Process 19 71.20±3.22d Process 28 79.12±0.93cd
Process 20 75.40±0.59cd Process 29 75.69±2.88d
Process 21 79.34±2.33c Process 30 83.92±1.04c
Process 22 86.41±1.28b Process 31 87.71±2.81bc
Process 23 92.26±0.57ab Process 32 90.35±3.30b
Process 24 95.49±4.19a Process 33 94.98±0.76a
Process 25 91.67±1.65ab Process 34 91.31±4.55ab
Process 26 93.36±0.62ab Process 35 93.58±0.48ab
Process 27 97.10±2.78a Process 36 96.50±1.81a
CK3 0.80±0.35e CK4 0.02±0.01e
The Be very effective that process 28-36 prevents and treats wild cabbage root-knot nematode is better than CK4, the effect of process 31-33 is significantly better than again processing 28-30 on the whole, with process 34-36 on the whole without significant difference, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 2.5:1.5:0.75 is by every 667m 2the control efficiency that 5-15kg uses significantly be better than the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag mass ratio be that the compatibility of 2.0:1.0:0.5 is by every 667m 2the control efficiency that 5-15kg uses, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 2.0:1.0:0.5 is by every 667m 2it is the lower limit of its compatibility concentration that 5kg uses.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is the compatibility of 2.5:1.5:0.75 and the fresh leaf of thunder godvine, the mass ratio of the fresh leaf of polygonum flaccidum, leached tea oil slag be its control efficiency of compatibility of 3.0:2.0:1.0 on the whole without significant difference, namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the compatibility of 3.0:2.0:1.0 is by every 667m 2it is the upper limit of its compatibility concentration that 15kg uses.Consider from cost and control efficiency 2 aspects, the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is the compatibility of 2.5:1.5:0.75, and it is cost-saving, and raw material, usage amount are all moderate, and control efficiency is good again, be good optimization formula ratio and amount of application.
From the above test to capsicum, cucumber, tomato and wild cabbage four kinds of root knot nematodes, the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.0 ~ 3.0:1.0 ~ 2.0:0.5 ~ 1.0 are by every 667m 25-15kg uses the upper limit and lower limit that include its control efficiency.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 210kg uses, and it is cost-saving, and raw material, usage amount are all moderate, and control efficiency is good again, is good optimization formula ratio and amount of application.From table 1-2 and the comparative analysis showing 3-4, same by every 667m 2the control efficiency of 5-15kg amount of application, the control efficiency of its table 3-4 is significantly better than the control efficiency showing 1-2, and namely the control efficiency of ingredients is significantly better than the control efficiency of single dose; And the control efficiency of ingredients is not equal to the summation of the control efficiency of each single dose of composition ingredients, the control efficiency of ingredients is greater than the summation of the control efficiency of each single dose of composition ingredients, namely the compatibility of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag has synergy, and the control efficiency namely after its compatibility has synergistic effect.
auxiliary agent is tested
Auxiliary agent is tested mainly qualitative and quantitative and is measured auxiliary agent to the impact of the control efficiency of whole ingredients.
materials and methods
3.1.1 test material
Test vegetable species is capsicum, cucumber, tomato and wild cabbage four kinds of vegetables.Test ingredients is the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, and auxiliary agent is leached tea oil slag.
test method
Test is carried out in proving ground, Yiyang.The plot often sending out root-knot nematode former years is selected to carry out.Control efficiency test is carried out when the root-knot nematode of capsicum, cucumber, tomato and wild cabbage occurs; Contrast is located at same plot.When test effect mainly occurs with same root knot nematode, after watering dispenser agent, count and statistical analysis with the percentage that root knot quantity reduces, contrast (CK) is watered with water spot cave and is executed.Capsicum, cucumber, tomato and wild cabbage four kinds of vegetables, when root-knot nematode is broken out, are selected to carry out dispenser process at cloudy day or fine day.Each treatment agent is attacked a vital point to water by the sprayer that 15 times of liquid (referring to amount of water) spend shower nozzle and is executed.Water to use in 24h and do not water or apply fertilizer, carry out during 15d observing and count.Conventional fertilizer water management after 24h.Test arranges 12 process, 4 contrasts, each process 3 repetition, each repetition 1.0m 2, random distribution.Percentage is reduced as treatment effect, simultaneously in conjunction with field observation using root knot quantity.The amount of application of each vegetables is shown in the following description to each process.Process 1: the test dose of capsicum compares---the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 210kg uses; Process 2: the test dose of capsicum compares---the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum is that 2.5:1.5 is by every 667m 210kg uses; Process 3: the test dose of capsicum compares---the mass ratio of water, leached tea oil slag is that 10.0:1.0 is by every 667m 210kg uses; CK1: the contrast water spot cave of capsicum is watered and executed.Process 4: the test dose of cucumber compares---the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 210kg uses; Process 5: the test dose of cucumber compares---the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum is that 2.5:1.5 is by every 667m 210kg uses; Process 6: the test dose of cucumber compares---the mass ratio of water, leached tea oil slag is that 10.0:1.0 is by every 667m 210kg uses; CK2: the contrast water spot cave of cucumber is watered and executed.Process 7: the test dose of tomato compares---the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 210kg uses; Process 8: the test dose of tomato compares---the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum is that 2.5:1.5 is by every 667m 210kg uses; Process 9: the test dose of tomato compares---the mass ratio of water, leached tea oil slag is that 10.0:1.0 is by every 667m 210kg uses; CK3: the contrast water spot cave of tomato is watered and executed.Process 10: the test dose of wild cabbage compares---the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that 2.5:1.5:0.75 is by every 667m 210kg uses; Process 11: the test dose of wild cabbage compares---the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum is that 2.5:1.5 is by every 667m 210kg uses; Process 12: the test dose of wild cabbage compares---the mass ratio of water, leached tea oil slag is that 10.0:1.0 is by every 667m 210kg uses; CK4: the contrast water spot cave of wild cabbage is watered and executed.
statistical analysis
SAS(6.12 is adopted to test data) carry out statistical analysis.As known from Table 5, the Be very effective of process 1 control capsicum root-knot nematode is better than CK1 and process 2-3, and namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the ingredients of 2.5:1.5:0.75 compatibility is by every 667m 2it is best that 10kg waters the control efficiency executed, secondly for the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum is the control efficiency of the ingredients of 2.5:1.5 compatibility.Test shows, the control efficiency that compatibility has the control efficiency of auxiliary agent to be significantly better than without auxiliary agent.Illustrate thus, auxiliary agent leached tea oil slag is prevented and treated root-knot nematode on capsicum and is had significant synergies in this ingredients.
root knot number slip (unit: %) during table 5. auxiliary agent test process 15d on capsicum, cucumber
Process 24h Process 24h
Process 1 95.26±0.97a Process 4 96.09±2.38a
Process 2 73.49±2.64b Process 5 80.92±1.67b
Process 3 8.89±0.30c Process 6 5.90±0.58c
CK1 1.69±0.09d CK2 0.52±0.17d
On process 4 control cucumber, the Be very effective of root-knot nematode is better than CK2 and process 5-6, and namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the ingredients of 2.5:1.5:0.75 compatibility is by every 667m 2it is best that 10kg waters the control efficiency executed, secondly for the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum is the control efficiency of 2.5:1.5 compatibility.Test shows, only has the control efficiency of auxiliary agent leached tea oil slag far away from the control efficiency of ingredients process 4-5; Without the control efficiency of the process 5 of the ingredients of auxiliary agent again far away from the control efficiency of process 4 having auxiliary agent ingredients.Illustrate thus, auxiliary agent leached tea oil slag is prevented and treated root-knot nematode on cucumber and is had significant synergies in this ingredients.
As known from Table 6, the Be very effective of process 7 control tomato root-knot eelworm is better than CK3 and process 8-9, and the effect of process 8 is significantly better than process 9 again, and namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the ingredients of 2.5:1.5:0.75 compatibility is by every 667m 2it is best that 10kg waters the control efficiency executed, secondly for the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum is the control efficiency of the ingredients of 3.5:1.5 compatibility.Test shows, only has the control efficiency of auxiliary agent leached tea oil slag far away from the control efficiency of ingredients process 7-8; Without the control efficiency of the process 8 of the ingredients of auxiliary agent again far away from the control efficiency of process 7 having auxiliary agent ingredients.Illustrate thus, auxiliary agent leached tea oil slag is used for preventing and treating tomato root-knot eelworm in this ingredients and has significant synergies.
root knot number slip (unit: %) during table 6. auxiliary agent test process 15d on tomato, wild cabbage
Process 24h Process 24h
Process 7 92.26±3.19a Process 10 90.35±4.81a
Process 8 84.16±0.82b Process 11 79.84±1.64b
Process 9 8.44±1.41c Process 12 7.51±1.37c
CK3 0.80±0.34d CK4 0.02±0.01d
The Be very effective of process 10 control wild cabbage root-knot nematode is better than CK4 and process 11-12, and the effect of process 11 is significantly better than process 12 again, and namely the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is that the ingredients of 2.5:1.5:0.75 compatibility is by every 667m 2it is best that 10kg waters the control efficiency executed, secondly for the mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum is the control efficiency of the ingredients of 3.5:1.5 compatibility.Test shows, only has the control efficiency of auxiliary agent leached tea oil slag far away from the control efficiency of ingredients process 10-11; Without the control efficiency of the process 11 of the ingredients of auxiliary agent again far away from the control efficiency of process 10 having auxiliary agent ingredients.Illustrate thus, auxiliary agent leached tea oil slag is used for preventing and treating wild cabbage root-knot nematode in this ingredients and has significant synergies.
More than test shows (table 5-6), and the difference on effect of adding assistant and not adding assistant is remarkable, and the Be very effective of adding assistant is better than the effect of not adding assistant, and its Be very effective of adding assistant does not reduce.Test also shows (table 1-6), and the organic vegetable root knot nematode control fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag compatibility whether effect is all significantly higher than CK; The mass ratio of auxiliary agent leached tea oil slag, the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum is that the ingredients of 0.75:2.5:1.5 compatibility is by every 667m 2it is best that 10kg waters the control efficiency executed, and its compatibility is best compatibility (table 1-6).Test shows, after using 15d, its main functional component wilfordine, general flavone, Tea Saponin etc. do not detect, and period does not descend moderate rain or heavy rain; After using, period following moderate rain or heavy rain more than 1 time, after using 10d, its main functional component wilfordine, general flavone, Tea Saponin etc. do not detect.Thunder godvine ( tripterygium wilfordiihook. f.), Celastraceae, tripterygium plant.Another name is herba fibraureae recisae, yellow cured rattan etc.Liana shrub, main product is in China, Korea, Japan, and China is mainly distributed in various places and southwest on the south the Yangtze river basin, main product in Fujian, Zhejiang, Anhui, the ground such as Henan.In being born in the shade many wet hillside, mountain valley, small stream limit shrub.The hillside that happiness is comparatively shady and cool, the sandy soil deep with slant acidity, soil layer or the most suitable growth of yellow soil.Bitter, pungent, cool in nature, poison greatly.Thunder God Calamus liana shrub, high 1-3 rice, sprig brownish red, has the thin rib of 4-6 root, by Mi Mao and fine and closely woven hole skin.Elliptic leaf shape, the ellipse of falling ovum, rectangular ellipse or avette, long 4-7.5 centimetre, wide 3-4 centimetre, the anxious point of tip or short gradually point, the wealthy wedge shape of base portion or circle, there is serration at edge, lateral vein 4-7 couple, slightly goes up curved after reaching leaf margin; The long 5-8 millimeter of petiole, close by rust hair.Circular cone cyme is narrower, long 5-7 centimetre, and wide 3-4 centimetre has 3-5 branch usually.The oval shape of samara, long 1-1.5 centimetre, diameter 1-1.2 centimetre, seed buttress shaft shape, reaches 10 millimeters.Thunder godvine bitter, pungent, cool in nature, poison greatly, has clearing heat and detoxicating, dispelling wind and removing obstruction in the meridians, relaxes the muscles and stimulate the blood circulation, swelling and pain relieving, the effect such as desinsection hemostasis, returns liver, kidney channel, effect is dispelled rheumatism, removes obstruction in channels to relieve pain, swelling and pain relieving, removing toxic substances desinsection.The traditional Chinese medical science is used for the treatment of lepra reaction, rheumatoid arthritis, pulmonary tuberculosis and other chronic lung diseases etc.Thunder godvine has the effects such as antitumor, anti-inflammatory, but it is very toxic, so thunder godvine is restricted in application medically always.Its main chemical of thunder godvine is: root is containing wilfordine (wilfordine), wilforine (wilforine), Wilforgine (wilforgine), Wilfortrine and wilfortrine (wil-skin acid amides (celacinnine), celastrus orbiculatus furancarboxylic acid amine (celafurine), celastrus orbiculatus benzyl acid amides (celagbenzine), Triptolide (wilforlide) A, B, triptonoterpenol (triptonoterpenol), 16-hydroxytriptolide (16-hydroxytripto-lide), triptolide and triptolide (triptolide), table Triptolide triol (epitriptriolide), thunder kaurene lactone (tripterifordine), mapping-Lei kaurene lactone (antriptolactone), wilfordic acid (tripterygic acid), straight wedge oxalic acid (orthosphenic acid), cupreol (β-sitosterol) and recklessly licopersicin (daucosterol).Chemistry and the Pharmacological Results of thunder godvine show, its active ingredient has Diterpenes, triterpenes and alkaloid compound etc.Diterpene-kind compound in thunder godvine belongs to abietane-type, there is α, β-unsaturated lactone structure, pharmacological research confirms that it is the bioactive main component of thunder godvine, mainly comprises: triptolide, NSC-163063, Triptolide triol, NSC-163063, Triptolide tetrol.Triterpene compound in thunder godvine has: wilforlide A, Wiofolide B, Orthosphenic acid A, Orthosphenic acid B, tripterygone, Celastrol etc.Polygonum flaccidum ( polygonum flaccidummeissn.) be polygonaceae Polygonum, another name: class burnt grass, flaccid knotweed herb etc., annual herb plant, high 60 ~ 90cm, complete stool scatters gland point and young pilose antler.Stem is upright, or throw oneself on the ground in bottom, usual aubergine, and joint expands, and leaf alternate, has short handle.The wide lanceolar of blade, tip is gradually sharp, base portion wedge shape, two sides by coarse wool, above bottle green, have splay blackspot, ocrea film quality, edge growth seta.Spike is born in Zhi Ding, and bennet is elongated, long 6 ~ 12cm, sagging, flower thinning; Perianth 5 drastic crack, white, scatter green gland point, top takes on a red color; Stamen 7 ~ 8; Ovary 1 Room, style 3 pieces.Achene 3 rib, outsourcing persitent perianth.Summer at florescence.At the florescence in summer, aciculiform blade is, has splayed blackspot, spends as spike sequence is born in Zhi Ding.Whole nation most area all has distribution, is born in nearly waterside, dark and damp place.Polygonum flaccidum has expelling wind and removing dampness, blood stasis removing analgesic, removing toxicity for detumescence, effect of killing parasites to relieve itshing, and the traditional Chinese medical science is used for the treatment of dysentery, gastroenteritis, diarrhoea, rheumatic arthritis, treating swelling and pain by traumatic injury, functional uterine bleeding; Venomous snake bite is controlled in external application, skin eczema etc.Herb is containing containing protein, organic acid, carbohydrate, tannin, flavones monomer rutin (Rutin), Hyperoside (Hyperin), quercetin (Quercetin), Isorhamnetin (Isorhamnetin) and kaempferol (Kaempferol) and volatile oil n-decane, a small amount of nopinene, citrene and n-undecane etc., slightly poisonous.Oil tea ( camellia oleiferaabel.) Theaceae, Camellia is belonged to, another name: tea subtree, tea oil tree etc.Evergreen dungarunga.Because of its seed can extract oil (tea oil) edible, therefore named.Shrub or middle arbor; Spray has coarse wool.Leaf keratin, oval, Long Circle or obovate, tip is sharp and have blunt nosed, sometimes gradually sharp or blunt, base portion wedge shape, long 5-7 centimetre, wide 2-4 centimetre, sometimes longer, above bottle green, shinny, middle arteries and veins has coarse wool or pubescence, below light green color, become mildewed without hair or middle arteries and veins, lateral vein can be shown in above, and below not clearly, there is serration at edge, sometimes have a cognate, the long 4-8 millimeter of petiole, has coarse wool.Tea oil tree is one of large woody oleiferous plants in the world four, and the high mountain of its growth in southern china subtropical zone and hilly country are the senior oil plants of the distinctive a kind of pure natural of China.China extensively cultivates from the Yangtze river basin to various places, south China.Mainly concentrate on Zhejiang, Jiangxi, Henan, Hunan, Guang Xiwu province.Tea oil look clear taste is fragrant, and nutritious, storage tolerance is high-quality edible oil; Also can be used as lubricating oil, antirust oil for industry.Tea cake is agricultural chemicals, is again fertilizer, can improve farmland water-holding capacity and control rice pest.Containing Tea Saponin, tea seed polysaccharide, tea seed albumen etc. in leached tea oil slag, they are all the raw materials of chemical industry, light industry, food, feed industry product etc.
Test shows, the main functional component wilfordine etc. of the fresh leaf of thunder godvine has good stomach poison function, the functional components such as the general flavone+volatile oil n-decane of the fresh leaf of polygonum flaccidum not only have good action of contace poison, can also promote that the functional components such as wilfordine are in the speed of root knot nematode toxicity in vivo effect, the two has well collaborative, synergistic effect.The main functional component of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum have root knot nematode good to tag, interior suction, the toxic action such as stomach toxicity.The functional components such as the Tea Saponin of leached tea oil slag not only can effectively dissolve the functional component such as wilfordine, general flavone, significantly improve the toxic action to the tagging of root knot nematode, interior suction, stomach toxicity etc., Tea Saponins etc. can also form soap-film, enhancing the toxic action of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, is a kind of well auxiliary agent.
Embodiment
Following embodiment and embodiment are intended to further illustrate the present invention, instead of limitation of the present invention.
Water the preparation of root agent stoste: the fresh leaf of material thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag all should be selected from free of contamination areas of environmental condition such as soil, water, air, soil, water body and fertilizer etc. also want heavy metal free to pollute, thunder godvine, polygonum flaccidum, tea oil tree system self-sow form, and do not use the mountain area of chemical fertilizer, chemical pesticide.Tripterygium leaf, polygonum flaccidum leaf all use fresh and alive material, and leached tea oil slag is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, do not need washing.Leached tea oil slag is smashed to pieces, in leached tea oil slag with hammer: the ratio of water=1:10 is soaked in water, after adding a cover fermentation 15-20d, 15-20d after mixing, the ratio that fresh for thunder godvine leaf, the fresh leaf of polygonum flaccidum describe in next section is respectively soaked in 3-5d in leached tea oil slag zymotic fluid.The fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag three components is 2.0 ~ 3.0:1.0 ~ 2.0:0.5 ~ 1.0, and the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and leached tea oil slag is auxiliary agent.Add a cover and soak after 3-5d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be mother liquor (stoste), can preserve 1-6 month.When breaking out root-knot nematode, lid being opened, taking out stoste, by every 667m 2the sprayer that 15 times of liquid (referring to amount of water) of 5 ~ 15kg stoste, 3 concentration gradients spend shower nozzle is attacked a vital point to water and is executed.
The most preferred mass ratio of the fresh leaf of above three components thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is 2.5:1.5:0.75, when breaking out root-knot nematode, lid is opened, and takes out stoste, by every 667m 25 ~ 15kg stoste, 15 times of liquid (referring to amount of water) are watered and are executed.Embodiment is implemented in proving ground, Yiyang, selects the plot often sending out root-knot nematode former years to carry out.Prevent and treat when capsicum, cucumber, tomato and wild cabbage break out root-knot nematode; Contrast is located at same plot.When effect is mainly with same vegetables outburst root-knot nematode, after watering dispenser agent, count and statistical analysis with the percentage that root knot quantity reduces, contrast (CK) is watered with water and is executed.Capsicum, cucumber, tomato and wild cabbage four kinds of vegetables, when breaking out root-knot nematode, are selected to carry out dispenser process at cloudy day or fine day.Water to use in 24h and do not water or apply fertilizer, carry out during 15d observing and count.Conventional fertilizer water management after 24h.
embodiment 1:
Water the preparation of root agent stoste: the fresh leaf of material thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag all should be selected from free of contamination areas of environmental condition such as soil, water, air, soil, water body and fertilizer etc. also want heavy metal free to pollute, thunder godvine, polygonum flaccidum, tea oil tree system self-sow form, and do not use the mountain area of chemical fertilizer, chemical pesticide.Tripterygium leaf, polygonum flaccidum leaf all use fresh and alive material, and leached tea oil slag is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, do not need washing.Leached tea oil slag is smashed to pieces, in leached tea oil slag with hammer: the ratio of water=1:10 is soaked in water, after adding a cover fermentation 15-20d, 15-20d after mixing, the ratio that fresh for thunder godvine leaf, the fresh leaf of polygonum flaccidum describe in next section is respectively soaked in 3-5d in leached tea oil slag zymotic fluid.The fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag three components is 2.0:1.0:0.5, and the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and leached tea oil slag is auxiliary agent.Add a cover and soak after 3-5d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be mother liquor (stoste), can preserve 1-6 month.When breaking out root-knot nematode, lid being opened, taking out stoste, by every 667m 2the sprayer that 5kg stoste 15 times of liquid (referring to amount of water) spend shower nozzle is attacked a vital point to water and is executed.When breaking out root-knot nematode, lid being opened, taking out stoste, by every 667m 25kg stoste 15 times of liquid (referring to amount of water) are watered and are executed.Embodiment is implemented in proving ground, Yiyang, selects the plot of Chang Fagen in former years border insect insect pest to carry out.Prevent and treat when capsicum, cucumber, tomato and wild cabbage break out root-knot nematode; Contrast is located at same plot.When effect is mainly with same vegetables outburst root-knot nematode, after watering dispenser agent, count and statistical analysis with the percentage that root knot quantity reduces, contrast (CK) is watered with water and is executed.Capsicum, cucumber, tomato and wild cabbage four kinds of vegetables, when breaking out root-knot nematode, are selected to carry out dispenser process at cloudy day or fine day.Water to use in 24h and do not water or apply fertilizer, carry out during 15d observing and count.Conventional fertilizer water management after 24h.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag three components is that 2.0:1.0:0.5 is by every 667m 25kg control agent stoste 15 times of liquid water the control efficiency executed.When processing 15d, the percentage that root knot quantity reduces is:
The percentage that pepper root footing amount reduces is 76.03%, CK is 1.69%, and control efficiency is remarkable; The percentage that cucumber root footing amount reduces is 73.24%, CK is 0.52%, and control efficiency is remarkable; The percentage that tomato root footing amount reduces is 71.20%, CK is 0.80%, and control efficiency is remarkable; The percentage that wild cabbage root knot quantity reduces is 79.12%, CK is 0.02%, and control efficiency is remarkable.
Result of implementation shows, organic vegetable waters the fresh leaf of root agent thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag compatibility mass ratio are that 2.0:1.0:0.5 is by every 667m 2it is remarkable that 5kg control agent stoste 15 times of liquid water the control efficiency executed.
embodiment 2:
Water the preparation of root agent stoste: the fresh leaf of material thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag all should be selected from free of contamination areas of environmental condition such as soil, water, air, soil, water body and fertilizer etc. also want heavy metal free to pollute, thunder godvine, polygonum flaccidum, tea oil tree system self-sow form, and do not use the mountain area of chemical fertilizer, chemical pesticide.Tripterygium leaf, polygonum flaccidum leaf all use fresh and alive material, and leached tea oil slag is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, do not need washing.Leached tea oil slag is smashed to pieces, in leached tea oil slag with hammer: the ratio of water=1:10 is soaked in water, after adding a cover fermentation 15-20d, 15-20d after mixing, the ratio that fresh for thunder godvine leaf, the fresh leaf of polygonum flaccidum describe in next section is respectively soaked in 3-5d in leached tea oil slag zymotic fluid.The fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag three components is 2.5:1.5:0.75, and the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and leached tea oil slag is auxiliary agent.Add a cover and soak after 3-5d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be mother liquor (stoste), can preserve 1-6 month.When breaking out root-knot nematode, lid being opened, taking out stoste, by every 667m 2the sprayer that 10kg stoste 15 times of liquid (referring to amount of water) spend shower nozzle is attacked a vital point to water and is executed.When breaking out root-knot nematode, lid being opened, taking out stoste, by every 667m 210kg stoste 15 times of liquid (referring to amount of water) are watered and are executed.Embodiment is implemented in proving ground, Yiyang, selects the plot of Chang Fagen in former years border insect insect pest to carry out.Prevent and treat when capsicum, cucumber, tomato and wild cabbage break out root-knot nematode; Contrast is located at same plot.When effect is mainly with same vegetables outburst root-knot nematode, after watering dispenser agent, count and statistical analysis with the percentage that root knot quantity reduces, contrast (CK) is watered with water and is executed.Capsicum, cucumber, tomato and wild cabbage four kinds of vegetables, when breaking out root-knot nematode, are selected to carry out dispenser process at cloudy day or fine day.Water to use in 24h and do not water or apply fertilizer, carry out during 15d observing and count.Conventional fertilizer water management after 24h.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag three components is that 2.5:1.5:0.75 is by every 667m 210kg control agent stoste 15 times of liquid water the control efficiency executed.When processing 15d, the percentage that root knot quantity reduces is:
The percentage that pepper root footing amount reduces is 95.26%, CK is 1.69%, and control efficiency is remarkable; The percentage that cucumber root footing amount reduces is 96.09%, CK is 0.52%, and control efficiency is remarkable; The percentage that tomato root footing amount reduces is 92.26%, CK is 0.80%, and control efficiency is remarkable; The percentage that wild cabbage root knot quantity reduces is 90.35%, CK is 0.02%, and control efficiency is remarkable.
Result of implementation shows, organic vegetable waters the fresh leaf of root agent thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag compatibility mass ratio are that 2.5:1.5:0.75 is by every 667m 2it is remarkable that 10kg control agent stoste 15 times of liquid water the control efficiency executed.
embodiment 3:
Water the preparation of root agent stoste: the fresh leaf of material thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag all should be selected from free of contamination areas of environmental condition such as soil, water, air, soil, water body and fertilizer etc. also want heavy metal free to pollute, thunder godvine, polygonum flaccidum, tea oil tree system self-sow form, and do not use the mountain area of chemical fertilizer, chemical pesticide.Tripterygium leaf, polygonum flaccidum leaf all use fresh and alive material, and leached tea oil slag is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium leaf, polygonum flaccidum leaf is removed during preparation, do not need washing.Leached tea oil slag is smashed to pieces, in leached tea oil slag with hammer: the ratio of water=1:10 is soaked in water, after adding a cover fermentation 15-20d, 15-20d after mixing, the ratio that fresh for thunder godvine leaf, the fresh leaf of polygonum flaccidum describe in next section is respectively soaked in 3-5d in leached tea oil slag zymotic fluid.The fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag three components is 3.0:2.0:1.0, and the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, and leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and leached tea oil slag is auxiliary agent.Add a cover and soak after 3-5d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be mother liquor (stoste), can preserve 1-6 month.When breaking out root-knot nematode, lid being opened, taking out stoste, by every 667m 2the sprayer that 15kg stoste 15 times of liquid (referring to amount of water) spend shower nozzle is attacked a vital point to water and is executed.When breaking out root-knot nematode, lid being opened, taking out stoste, by every 667m 215kg stoste 15 times of liquid (referring to amount of water) are watered and are executed.Embodiment is implemented in proving ground, Yiyang, selects the plot of Chang Fagen in former years border insect insect pest to carry out.Prevent and treat when capsicum, cucumber, tomato and wild cabbage break out root-knot nematode; Contrast is located at same plot.When effect is mainly with same vegetables outburst root-knot nematode, after watering dispenser agent, count and statistical analysis with the percentage that root knot quantity reduces, contrast (CK) is watered with water and is executed.Capsicum, cucumber, tomato and wild cabbage four kinds of vegetables, when breaking out root-knot nematode, are selected to carry out dispenser process at cloudy day or fine day.Water to use in 24h and do not water or apply fertilizer, carry out during 15d observing and count.Conventional fertilizer water management after 24h.The mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag three components is that 3.0:2.0:1.0 is by every 667m 215kg control agent stoste 15 times of liquid water the control efficiency executed.When processing 15d, the percentage that root knot quantity reduces is:
The percentage that pepper root footing amount reduces is 98.23%, CK is 1.69%, and control efficiency is remarkable; The percentage that cucumber root footing amount reduces is 97.41%, CK is 0.52%, and control efficiency is remarkable; The percentage that tomato root footing amount reduces is 97.10%, CK is 0.80%, and control efficiency is remarkable; The percentage that wild cabbage root knot quantity reduces is 96.50%, CK is 0.02%, and control efficiency is remarkable.
Result of implementation shows, organic vegetable waters the fresh leaf of root agent thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag compatibility mass ratio are that 3.0:2.0:1.0 is by every 667m 2it is remarkable that 15kg control agent stoste 15 times of liquid water the control efficiency executed.

Claims (7)

1. organic vegetable waters a root agent, it is characterized in that, described organic vegetable waters root agent by the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum with auxiliary agent leached tea oil slag compatibility is brewed forms.
2. a kind of organic vegetable according to claim 1 waters root agent, it is characterized in that, the compatibility mass ratio of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum and auxiliary agent leached tea oil slag is 2.0 ~ 3.0:1.0 ~ 2.0:0.5 ~ 1.0.
3. a kind of organic vegetable according to claim 1 and 2 waters root agent, it is characterized in that, the preferred mass of the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum, leached tea oil slag is than being 2.5:1.5:0.75.
4. the organic vegetable root knot nematode control agent according to claims 1 to 3, it is characterized in that, the fresh leaf of thunder godvine, the fresh leaf of polygonum flaccidum are basis by fresh, leached tea oil slag is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, leached tea oil slag need first in leached tea oil slag: water is that the ratio of 1:10 adds water and makes leached tea oil slag zymotic fluid, leached tea oil slag zymotic fluid is that auxiliary agent doubles as brewed medium, and the brewed liquid of this compatibility is stoste.
5. water root agent according to the organic vegetable described in claim 2 to 4, it is characterized in that, every 667m is pressed to the control of root knot nematode 2water with 5 ~ 15kg stoste, 15 times of liquid and execute.
6. using method according to claim 5, is characterized in that, presses every 667m to the control of root knot nematode 210kg stoste 15 times of liquid water and execute is more excellent and the amount of application of economy.
7. according to the using method described in claim 5 to 6, it is characterized in that, before root knot nematode outburst or when breaking out, select to carry out watering executing at cloudy day or fine day, use in rear 24h and do not water or apply fertilizer, conventional fertilizer water management after 24h, has descended moderate rain or heavy rain if used within rear 24h, has then needed again to process after rain.
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