CN104782674B - A kind of organic vegetable control of aphids agent and its application method - Google Patents
A kind of organic vegetable control of aphids agent and its application method Download PDFInfo
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Abstract
The invention discloses a kind of organic vegetable control of aphids agent and its application method, described control agent is by tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake compatibility is brewed forms, and its tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.0~4.0:1.0~2.0:6.0~8.0:0.5~1.0 compatibility mixture, is preferably in a proportion of 3.5:1.5:7.5:0.75, tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is calculated by the dry cake weight of 1~30d moisture absorption of not going mouldy, human urine is pressed the pure urine weight for depositing 1~30d undoped with excrement etc. and calculated, wherein human urine is brewed medium, and Oil-tea-cake is auxiliary agent, and the brewed liquid of this compatibility is stoste.This compatibility agent is notable to the prevention effect of organic vegetable aphid, and with synergy.Preventing and treating to insect pest is pressed per 667m25~15kg stostes, 10 times of liquid sprayings, can comparatively fast kill aphid, prevent insect pest from spreading, moreover it is possible to supplement the nutrition such as nitrogen, accelerate the recovery of insect pest strain.The production of this control agent is upper easy to use, and simple to operate, cost is low, and prevention effect is notable.
Description
Technical field
The invention belongs to agriculture practical new technology research and development and application；The present invention relates to a kind of organic vegetable control of aphids agent
Research and development and production application method.
Background technology
Vegetable aphid is commonly called as aphid, aphid etc., belongs to homoptera pest.Vegetable aphid be crops primary pest it
One, species is more, and generation is more, and breeding is fast, harm weight.Vegetable aphid mainly has black peach aphid（Also referred to as cigarette aphid）, radish aphid, melon aphid（
Claim cotten aphid）, brevicoryne brassicae, bean aphid（Also referred to as Aphis medicaginis, aphis craccivora）Deng, with adult aphid, larvae aphid thorn inhale host's juice, cause plant strain growth
It is bad, leaf curl can be made when aggrieved serious, branches and leaves jaundice deformation, bud is ruined, and the florescence shortens, and flower appearance loses lustre, growth retardation,
Complete stool withered death when serious, causes total crop failure；Meanwhile, can propagate various vegetables virosis again, induce vegetable disease, harm compared with
Greatly.Vegetable aphid is main intensive in vegetable seedling, tender leaf, stem and subaerial blade back with adult, nymph, and thorn inhales juice.1 year
There are generation in the four seasons, and breeding is most fast typically when temperature is at 29 DEG C or so.At present, mainly there is thing to the prevention and controls of vegetable aphid
Manage preventing and treating, chemical prevention, biological control etc..The physical control that tack coat traps and kills aphid is coated using crocus lath, natural enemy is utilized
The biological control advantage of anti-aphid is pollution-free, but its prevention effect is not thorough, strictly speaking simply a kind of to suppress, to some melons
The vegetables of the types such as dish, leaf vegetables, to its any damage, can all have a strong impact on its attractive in appearance and quality, and these vegetables are come
Say, it is impossible to have any damage.So, physical control and biological control have its limitation, that is, have a larger defect, and cost compared with
Height, this is also one of the reason for it is difficult to be widely popularized.At present, the two be preventing and treating supplementary means.Chemical prevention is as current
Using more universal synthetic organic pesticide Acetamiprid, imidacloprid etc., though preventing and treating ratio is more thoroughly, its toxicity is big, and residual harm is tight
Weight, is to forbid to use during organic vegetable is produced.The control of aphids agent of this organic vegetable overcomes disadvantages described above, both pollution-free, again
There is good prevention effect, cost is also very low, be a kind of good organic vegetable control of aphids agent.Insect pest is used before occurring, energy
Prevention occurs；Applied after generation, can comparatively fast kill aphid, prevent insect pest from spreading, plant restore normal growth soon.This organic vegetable
Control of aphids agent is that, through studying the achievement with production application for many years, it can both prevent, can be controlled again, and easy to use, simple to operate, into
This is low, and significantly, this is other can not be than about medicament or measure for prevention effect.
The content of the invention
The purpose of the present invention aims to provide a kind of organic vegetable control of aphids agent, and easy to use, simple to operate, cost is low,
Prevention effect is notable.Another object of the present invention aims to provide the preferably application method of above-mentioned organic vegetable control of aphids agent.
The purpose of the present invention is achieved in the following ways：
The control agent of the present invention is tripterygium root, polygonum flaccidum stem, Oil-tea-cake, human urine composition, and wherein Oil-tea-cake is mainly used as helping
Agent, plays a part of to promote its prevention effect, can be added according to production application, human urine is mainly used as brewed medium, also had
The effect of foliage dressing, accelerate the recovery of the normal growth and development after deinsectization.Inventor's research is found, adds auxiliary agent Oil-tea-cake energy
Play good synergistic effect.Need it is emphasized that the tripterygium root of this organic vegetable control of aphids agent, polygonum flaccidum stem, oil tea
Cake, human urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, and tripterygium wilfordii, polygonum flaccidum, tea oil tree system are natural
Growth is formed, and the mountain area of chemical fertilizer, chemical pesticide was not applied.Otherwise, the quality of organic vegetable will be influenceed.
The preparation method of this organic vegetable control of aphids agent is：Material tripterygium root, polygonum flaccidum stem, Oil-tea-cake, human urine all should
From from the free of contamination area of the environmental conditions such as soil, water, air, soil, water body and organic fertilizer etc. also want heavy metal free to pollute,
Tripterygium root, polygonum flaccidum stem, Oil-tea-cake use fresh and alive material, and human urine deposits 1-30d pure urine undoped with water, excrement etc..Oil-tea-cake
For the dry cake of 1-30d moisture absorption of not going mouldy.First the soil on tripterygium root, polygonum flaccidum stem is removed during preparation, washing is not needed.You
Afterwards, tripterygium root, polygonum flaccidum stem smash damage with hammer open and remove, be cut into the segment of 10cm length, Oil-tea-cake is pounded 5 × 5cm sizes with hammering into shape
Fritter, be soaked in proportion in urine, after mixing capping immersion 15-30d.Tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake
Calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, human urine is based on the pure urine weight that 1-30d is deposited undoped with water, excrement etc.
Calculate.Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of the component of Oil-tea-cake four are 3.0~4.0:1.0~2.0:6.0~8.0:0.5
~1.0, tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, human urine
Pure urine weight by 1-30d is deposited undoped with excrement etc. is calculated.Capping immersion 15-30d after mixing.After capping immersion 15-30d, 4
Layer filtered through gauze 2~3 times, collects filtrate, is put into container, seals immediately, is this organic vegetable control of aphids agent stoste,
It can preserve 1-6 months.This organic vegetable control of aphids agent is applied before insect pest occurs, and prevention is pressed per 667m25~10kg stostes
10 times of liquid sprayings；Applied after insect pest generation, by every 667m210~15kg stostes, 10 times of liquid sprayings, can comparatively fast kill aphid,
Prevent insect pest from spreading, moreover it is possible to supplement the nutrition such as nitrogen, accelerate to recover.Upper easy to use, simple to operate, the cost of this control agent production
Low, significantly, the shelf-life is longer, is packed with plastic bag sealing for prevention effect, can carry out factorial praluction, forms product.
The component tripterygium root of the above four, polygonum flaccidum stem, human urine, the most preferred mass ratio of Oil-tea-cake are 3.5:1.5:7.5:
0.75, tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, and human urine is pressed
The pure urine weight for depositing 1-30d undoped with water, excrement etc. is calculated.Capping immersion 15-30d after mixing.After capping immersion 15-30d,
4 layers of filtered through gauze 2~3 times, collects filtrate, is put into container, seals immediately, is that this organic vegetable control of aphids agent is former
Liquid, can be preserved 1-6 months.This organic vegetable control of aphids agent is applied before insect pest occurs, and prevention is pressed per 667m25~10kg is former
10 times of liquid sprayings of liquid；Applied after insect pest generation, by every 667m210~15kg stostes, 10 times of liquid sprayings, can comparatively fast kill aphid
Worm, prevents insect pest from spreading, moreover it is possible to supplement the nutrition such as nitrogen, accelerates the recovery of plant injury, promotes growth.In the production of this control agent
Easy to use, simple to operate, cost is low, and significantly, the shelf-life is longer, to stoste for prevention effect（That is mother liquor）Use plastic bag sealing bag
Dress, can carry out factorial praluction, form product.
The present invention employs matching somebody with somebody for suitable concentration from control agent tripterygium root, polygonum flaccidum stem, human urine and auxiliary agent Oil-tea-cake
5, commercial control agent is configured to, it can both prevent, can controlled again, it has significant preventive and therapeutic effect to organic vegetable aphid.Using this
Organic vegetable control of aphids is it compared with one of effective measures.This control agent is easy to use, simple to operate, and cost is low, preventing and treating effect
Significantly, this is other can not be than about medicament or measure for fruit.This organic vegetable control of aphids agent prescription is tripterygium root, peppery
Knotweed stem, human urine and auxiliary agent Oil-tea-cake, brewed, mother liquor plastic bag sealing packaging are mixed by the ratio of above-mentioned mass ratio, cool place is dried
Preserved at lucifuge, 6 months shelf-lifves.Tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake by 1-30d moisture absorption of not going mouldy
Dry cake weight is calculated, and human urine is pressed the pure urine weight for depositing 1-30d undoped with excrement etc. and calculated.The following is the comparison of its regulating effect
Experiment.The organic vegetable control of aphids agent of the present invention has no the report of like product at present, and the present invention is inventor by for many years
The result of the test that experimental study is drawn with production practices, and be verified in the production of long period, effect is preferable.And
The formula of the present invention also has technology content higher, and formulation is relatively stablized, and raw material sources enrich, easy to operate, such as develop into commodity,
It is cheap.Easy to use in production, significantly, use cost is low, is a kind of good organic vegetable aphid for prevention effect
Worm control agent.Organic vegetable control of aphids agent prescription can carry out factorial praluction, and its exploitation not only will commercially increase
A kind of new organic vegetable control of aphids agent, will also for current organic vegetable control of aphids effect it is not good the problem of one solution is provided
Approach certainly.
The present invention application method be：This organic vegetable control of aphids agent is applied before insect pest occurs, and prevention is pressed per 667m2
5~10kg stostes, 10 times of liquid sprayings；Applied after insect pest generation, by every 667m210~15kg stostes, 10 times of liquid, which are sprayed, is
Can, aphid can be comparatively fast killed, prevents insect pest from spreading, moreover it is possible to the nutrition such as nitrogen are supplemented, accelerates to recover.
The present invention brewing method be：First the soil on tripterygium root, polygonum flaccidum stem is removed during preparation, washing is not needed.You
Afterwards, tripterygium root, polygonum flaccidum stem smash damage with hammer open and remove, be cut into the segment of 10cm length, Oil-tea-cake is pounded 5 × 5cm sizes with hammering into shape
Fritter, be soaked in proportion in urine, after mixing capping immersion 15-30d.Tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake
Calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, human urine is pressed the pure urine weight for depositing 1-30d undoped with excrement etc. and calculated.
Tripterygium root, polygonum flaccidum stem, human urine, the compatibility mass ratio of the component of Oil-tea-cake four are 3.0~4.0:1.0~2.0:6.0~8.0:0.5
~1.0, tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, human urine
Pure urine weight by 1-30d is deposited undoped with excrement etc. is calculated.Capping immersion 15-30d after mixing.After capping immersion 15-30d, 4
Layer filtered through gauze 2~3 times, collects filtrate, is put into container, seals immediately, is this organic vegetable control of aphids agent stoste,
It can preserve 1-6 months.Using can not sprinkle water or apply fertilizer in rear 24h, conventional rich water quality management after 24h, if within using rear 24h
Rain, then need to handle again after rain.Experiment shows, after 15d, and vegetables are detected, the major function of its medicament
Composition is not detected, does not during which descend moderate rain or heavy rain；After, during which following moderate rain or heavy rain more than 1 time, after 7d, its main work(
Energy composition is not detected.
The purpose of the present invention is realized the following is inventor, is constantly selected and is had from suitable of the invention by experiment
The comparative test for the prevention effect that the raw material of machine vegetable aphid preventing and treating agent prescription and its proportioning of correlation are done.
Single group separating tests
Single group separating tests are mainly the single effect examination that each component of qualitative determination is prevented and treated organic vegetable aphid
Test.
Materials and methods
1.1.1 test material
Experiment vegetable species are from Chinese cabbage, cucumber, the four kinds of vegetables of radish and fresh kidney beans for being easiest to break out aphid.Experiment is with matching somebody with somebody
Agent is：Tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake.
Test method
The preparation of test material：Material tripterygium root, polygonum flaccidum stem, Oil-tea-cake, human urine all should be selected and come from soil, water, air
Etc. the free of contamination area of environmental condition, tripterygium root, polygonum flaccidum stem, Oil-tea-cake use fresh and alive material, and human urine is deposited undoped with excrement etc.
Put 1-30d pure urine.Oil-tea-cake is the dry cake of 1-30d moisture absorption of not going mouldy.First by the mud on tripterygium root, polygonum flaccidum stem during preparation
Soil removes, and does not need washing.Thereafter, tripterygium root, polygonum flaccidum stem smash damage with hammer open and remove, be cut into the segment of about 10cm length.With hammer handle
Oil-tea-cake is pounded the fritter of about 5 × 5cm sizes.Tripterygium root, polygonum flaccidum stem, Oil-tea-cake are soaked in the ratio of next segment description respectively
In urine, capping immersion 15-30d after mixing.Tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake by 1-30d suction of not going mouldy
Wet dry cake weight is calculated, and human urine is pressed the pure urine weight for depositing 1-30d undoped with excrement etc. and calculated.
Tripterygium root component prepares mother liquor by following mass ratio：Tripterygium root:Human urine=3.5:7.5（Wherein human urine is brewed
Medium, its quality is included into its mother liquor gross mass）.Polygonum flaccidum stem component prepares mother liquor, polygonum flaccidum stem by following mass ratio:Human urine=1.5:
7.5（Wherein human urine is brewed medium, and its quality is included into its mother liquor gross mass）.Oil-tea-cake component prepares female by following mass ratio
Liquid, Oil-tea-cake:Human urine=0.75:7.5（Wherein human urine is brewed medium, and its quality is included into its mother liquor gross mass）.Human urine component is adopted
With the human urine for having deposited 30d.Tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake by 1-30d moisture absorption of not going mouldy dry cake weight
Amount is calculated, and human urine is pressed the pure urine weight for depositing 30d undoped with water, excrement etc. and calculated.After capping immersion 15d, 4 layers of filtered through gauze 2
~3 times, filtrate is collected, container is put into, seals immediately, as female stoste of each component single dose, preserved 1-6 months.Quick-fried
When sending out aphid, lid is opened, each single dose stoste is taken out respectively（Human urine component takes out the human urine for having deposited 30d）, by every 667m2
5th, 10,10 times of liquid of 3 concentration gradients of 15kg stostes（Refer to amount of water）Spraying.
Experiment is carried out in Yiyang proving ground.The plot that aphid insect pest is often sent out from former years is carried out.When Chinese cabbage, cucumber, radish
Prevention effect experiment is carried out when being broken out with fresh kidney beans aphid；Control is located at same plot.Test effect mainly uses same vegetables
When aphid is broken out, after spraying medicament, counted and statistical analysis, compareed with the percentage of the aphid death rate（CK）Water spray.Chinese cabbage,
Cucumber, four kinds of vegetables of radish and fresh kidney beans are when aphid is broken out, and selection is handled in the morning or during the dusk.Each single dose is applied
Amount：Its mother liquor amount of application of tripterygium root component is pressed per 667m2Tri- kinds of concentration gradients of 5kg, 10kg, 15kg carry out spraying administration.It is peppery
Its mother liquor amount of application of knotweed stem component is pressed per 667m2Tri- kinds of concentration gradients of 5kg, 10kg, 15kg carry out spraying administration.Oil-tea-cake group
Its mother liquor amount of application is divided to press per 667m2Tri- kinds of concentration gradients of 5kg, 10kg, 15kg carry out spraying administration.Human urine component is by every
667m2Tri- kinds of concentration gradients of 5kg, 10kg, 15kg carry out spraying administration.Spraying, which is applied, does not sprinkle water or applies fertilizer in 24h, during 24h
Observed and counted.Conventional rich water quality management after 24h.Experiment sets 48 processing 4 to compare, each 3 repetitions of processing, often
It is individual to repeat 0.10m2, random distribution.Treatment effect is used as using Percent mortality.
Processing 1：The aphid of Chinese cabbage --- tripterygium root is pressed per 667m25kg is applied；Processing 2：The aphid of Chinese cabbage --- thunder
Public rattan root is pressed per 667m210kg is applied；Processing 3：The aphid of Chinese cabbage --- tripterygium root is pressed per 667m215kg is applied；Processing 4：
The aphid of Chinese cabbage --- polygonum flaccidum stem is pressed per 667m25kg is applied；Processing 5：The aphid of Chinese cabbage --- polygonum flaccidum stem is pressed per 667m2 10kg
Using；Processing 6：The aphid of Chinese cabbage --- polygonum flaccidum stem is pressed per 667m215kg is applied；Processing 7：The aphid of Chinese cabbage --- Oil-tea-cake
By every 667m25kg is applied；Processing 8：The aphid of Chinese cabbage --- Oil-tea-cake is pressed per 667m210kg is applied；Processing 9：The aphid of Chinese cabbage
Worm --- Oil-tea-cake is pressed per 667m215kg is applied；Processing 10：The aphid of Chinese cabbage --- human urine is pressed per 667m25kg is applied；Processing
11：The aphid of Chinese cabbage --- human urine is pressed per 667m210kg is applied；Processing 12：The aphid of Chinese cabbage --- human urine is pressed per 667m2
15kg is applied；CK1：The control of Chinese cabbage, spray pharmacy, does not spray water.Processing 13：The aphid of cucumber --- tripterygium root is pressed per 667m2
5kg is applied；Processing 14：The aphid of cucumber --- tripterygium root is pressed per 667m210kg is applied；Processing 15：The aphid of cucumber ---
Tripterygium root is pressed per 667m215kg is applied；Processing 16：The aphid of cucumber --- polygonum flaccidum stem is pressed per 667m25kg is applied；Processing
17：The aphid of cucumber --- polygonum flaccidum stem is pressed per 667m210kg is applied；Processing 18：The aphid of cucumber --- polygonum flaccidum stem is pressed per 667m2
15kg is applied；Processing 19：The aphid of cucumber --- Oil-tea-cake is pressed per 667m25kg is applied；Processing 20：The aphid of cucumber --- oil
Tea cake is pressed per 667m210kg is applied；Processing 21：The aphid of cucumber --- Oil-tea-cake is pressed per 667m215kg is applied；Processing 22：It is yellow
The aphid of melon --- human urine is pressed per 667m25kg is applied；Processing 23：The aphid of cucumber --- human urine is pressed per 667m210kg is applied；
Processing 24：The aphid of cucumber --- human urine is pressed per 667m215kg is applied；CK2：The control of cucumber, spray pharmacy, does not spray water.Processing
25：The aphid of radish --- tripterygium root is pressed per 667m25kg is applied；Processing 26：The aphid of radish --- tripterygium root is by every
667m210kg is applied；Processing 27：The aphid of radish --- tripterygium root is pressed per 667m215kg is applied；Processing 28：The aphid of radish
Worm --- polygonum flaccidum stem is pressed per 667m25kg is applied；Processing 29：The aphid of radish --- polygonum flaccidum stem is pressed per 667m210kg is applied；Place
Reason 30：The aphid of radish --- polygonum flaccidum stem is pressed per 667m215kg is applied；Processing 31：The aphid of radish --- Oil-tea-cake is pressed per 667m2
5kg is applied；Processing 32：The aphid of radish --- Oil-tea-cake is pressed per 667m210kg is applied；Processing 33：The aphid of radish --- oil tea
Cake is pressed per 667m215kg is applied；Processing 34：The aphid of radish --- human urine is pressed per 667m25kg is applied；Processing 35：The aphid of radish
Worm --- human urine is pressed per 667m210kg is applied；Processing 36：The aphid of radish --- human urine is pressed per 667m215kg is applied；CK3：Trailing plants
The control of fore-telling, spray pharmacy, does not spray water.Processing 37：The aphid of fresh kidney beans --- tripterygium root is pressed per 667m25kg is applied；Processing 38：
The aphid of fresh kidney beans --- tripterygium root is pressed per 667m210kg is applied；Processing 39：The aphid of fresh kidney beans --- tripterygium root is by every
667m215kg is applied；Processing 40：The aphid of fresh kidney beans --- polygonum flaccidum stem is pressed per 667m25kg is applied；Processing 41：The aphid of fresh kidney beans
Worm --- polygonum flaccidum stem is pressed per 667m210kg is applied；Processing 42：The aphid of fresh kidney beans --- polygonum flaccidum stem is pressed per 667m215kg is applied；
Processing 43：The aphid of fresh kidney beans --- Oil-tea-cake is pressed per 667m25kg is applied；Processing 44：The aphid of fresh kidney beans --- Oil-tea-cake is by every
667m210kg is applied；Processing 45：The aphid of fresh kidney beans --- Oil-tea-cake is pressed per 667m215kg is applied；Processing 46：The aphid of fresh kidney beans
Worm --- human urine is pressed per 667m25kg is applied；Processing 47：The aphid of fresh kidney beans --- human urine is pressed per 667m210kg is applied；Processing
48：The aphid of fresh kidney beans --- human urine is pressed per 667m215kg is applied；CK4：The control of fresh kidney beans, spray pharmacy, does not spray water.
Statistical analysis
SAS is used to test data（6.12）Carry out statistical analysis.The effect each handled after processing 24h is shown in Table 1-2.Examination
Result is tested to show（Table 1）, the aphid death rate of processing 3, which is significantly higher than, handles 1-2, illustrates that higher concentration tripterygium root prevents and treats Chinese cabbage
The effect of upper aphid is significantly better than the tripterygium root of low concentration.Processing 1-3 is significantly higher than other processing and CK1, i.e. Thunder God again
The effect of aphid is significantly better than polygonum flaccidum stem, Oil-tea-cake, the prevention effect of human urine on rattan root preventing and treating Chinese cabbage.
The effect of aphid is on various concentrations polygonum flaccidum stem preventing and treating Chinese cabbage：The aphid death rate of processing 6 is significantly higher than processing 4-
5, illustrate that the effect of aphid on higher concentration polygonum flaccidum stem preventing and treating Chinese cabbage is significantly better than the polygonum flaccidum stem of low concentration.4-6 is handled again to show
The effect write higher than aphid on processing 7-12 and CK1, i.e. polygonum flaccidum stem preventing and treating Chinese cabbage is significantly better than Oil-tea-cake, the preventing and treating effect of human urine
Really, but not as good as the prevention effect of tripterygium root.The effect of aphid is on various concentrations Oil-tea-cake preventing and treating Chinese cabbage：The place of higher concentration
Reason 9 and the processing 7-8 aphids death rate of low concentration are without significant difference, but processing 8-9 is significantly higher than processing 10-12 and CK1, i.e.,
The effect of aphid is significantly better than the prevention effect of human urine on Oil-tea-cake preventing and treating Chinese cabbage, but not as good as the preventing and treating of tripterygium root and polygonum flaccidum stem
Effect.The effect of aphid is on various concentrations human urine preventing and treating Chinese cabbage：10-12 and CK1 is handled without significant difference, i.e. human urine preventing and treating is white
The effect of aphid is not notable on dish.
Chinese cabbage during 1. one pack system test process 24h of table, the aphid death rate on cucumber（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 1 | 31.68±3.64b | Processing 13 | 31.85±3.25b |
| Processing 2 | 33.64±1.91b | Processing 14 | 34.08±2.25b |
| Processing 3 | 41.09±2.16a | Processing 15 | 42.53±2.04a |
| Processing 4 | 13.01±0.53d | Processing 16 | 13.72±3.25c |
| Processing 5 | 14.19±3.49d | Processing 17 | 14.81±1.97c |
| Processing 6 | 18.34±1.02c | Processing 18 | 15.17±2.61c |
| Processing 7 | 4.89±2.26ef | Processing 19 | 4.21±0.90de |
| Processing 8 | 5.15±1.68e | Processing 20 | 5.49±1.28de |
| Processing 9 | 6.02±0.78e | Processing 21 | 7.15±0.69d |
| Processing 10 | 3.08±1.38f | Processing 22 | 3.17±1.61e |
| Processing 11 | 4.26±1.10ef | Processing 23 | 3.54±0.91e |
| Processing 12 | 4.51±0.57ef | Processing 24 | 4.98±1.28de |
| CK1 | 3.03±0.33f | CK2 | 2.15±0.38e |
Note：Lowercase is 0.05 level, and the different letters of same row represent significant difference, similarly hereinafter.
The effect of aphid is on various concentrations tripterygium root preventing and treating cucumber：The aphid death rate of processing 15 is significantly higher than processing
13-14, illustrates that the effect of aphid on higher concentration tripterygium root preventing and treating cucumber is significantly better than the tripterygium root of low concentration.Processing
The effect that 13-15 is significantly higher than aphid on other processing and CK2, i.e. tripterygium root preventing and treating cucumber again is significantly better than polygonum flaccidum stem, oil
Tea cake, the prevention effect of human urine.
The effect of aphid is on various concentrations polygonum flaccidum stem preventing and treating cucumber：The 16-18 aphid death rate is handled without significant difference,
But processing 16-18 be significantly higher than processing 19-24 and CK2, i.e., polygonum flaccidum stem preventing and treating Chinese cabbage on aphid effect be significantly better than Oil-tea-cake,
The prevention effect of human urine, but not as good as the prevention effect of tripterygium root.The effect of aphid is on various concentrations Oil-tea-cake preventing and treating cucumber：
The 19-21 aphid death rate is handled without significant difference, but processing 21 is significantly higher than the oil tea of processing 22-23 and CK2, i.e. high concentration
The effect of aphid is significantly better than the prevention effect of human urine on cake preventing and treating Chinese cabbage, but not as good as the preventing and treating effect of tripterygium root and polygonum flaccidum stem
Really.The effect of aphid is on various concentrations human urine preventing and treating cucumber：22-24 and CK2 is handled without significant difference, i.e. human urine preventing and treating cucumber
The effect of upper aphid is not notable.
Result of the test shows（Table 2）, the aphid death rate of processing 27, which is significantly higher than, handles 25-26, illustrates higher concentration thunder
The effect of aphid is significantly better than the tripterygium root of low concentration on public rattan root preventing and treating radish.Processing 25-27 is significantly higher than its elsewhere again
The effect of aphid is significantly better than polygonum flaccidum stem, Oil-tea-cake, the prevention effect of human urine on reason and CK3, i.e. tripterygium root preventing and treating radish.
The effect of aphid is on various concentrations polygonum flaccidum stem preventing and treating radish：The processing 30 of higher concentration and the processing of low concentration
The 28-30 aphid death rates are without significant difference.But the processing 28-30 aphid death rate, which is significantly higher than, handles 31-36 and CK3, i.e., peppery
The effect of aphid is significantly better than Oil-tea-cake, the prevention effect of human urine on knotweed stem preventing and treating radish, but not as good as the preventing and treating effect of tripterygium root
Really.The effect of aphid is on various concentrations Oil-tea-cake preventing and treating radish：The processing 33 of higher concentration and the processing 31-32 of low concentration
The aphid death rate is without significant difference, but processing 31-33 is significantly higher than the effect of aphid on CK3, i.e. Oil-tea-cake preventing and treating radish significantly,
But not as good as the prevention effect of tripterygium root and polygonum flaccidum stem.The effect of aphid is on various concentrations human urine preventing and treating radish：Handle 34-36
It is not notable with effects of the CK3 without aphid on significant difference, i.e. human urine preventing and treating radish.
Radish during 2. one pack system test process 24h of table, the aphid death rate on fresh kidney beans（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 25 | 31.93±2.18b | Processing 37 | 24.27±0.80c |
| Processing 26 | 32.58±0.81b | Processing 38 | 30.15±1.41b |
| Processing 27 | 10.80±5.33a | Processing 39 | 51.87±1.67a |
| Processing 28 | 23.72±2.15c | Processing 40 | 20.43±4.47c |
| Processing 29 | 23.84±2.96c | Processing 41 | 21.39±2.15c |
| Processing 30 | 25.27±1.41c | Processing 42 | 24.80±0.62c |
| Processing 31 | 5.61±0.91de | Processing 43 | 4.07±1.27e |
| Processing 32 | 8.10±1.78d | Processing 44 | 7.64±0.78de |
| Processing 33 | 7.85±2.29d | Processing 45 | 9.81±1.90d |
| Processing 34 | 3.17±0.71e | Processing 46 | 2.90±1.02e |
| Processing 35 | 3.61±1.01e | Processing 47 | 3.17±1.27e |
| Processing 36 | 4.5±1.28de | Processing 48 | 3.64±0.58e |
| CK3 | 1.10±0.57e | CK4 | 3.25±0.39e |
The effect of aphid is on various concentrations tripterygium root preventing and treating fresh kidney beans：The aphid death rate of processing 39 is significantly higher than processing
37-38, illustrates that the effect of aphid on higher concentration tripterygium root preventing and treating fresh kidney beans is significantly better than the tripterygium root of low concentration.Processing
The effect that 37-39 is significantly higher than aphid on other processing and CK4, i.e. tripterygium root preventing and treating fresh kidney beans again is significantly better than polygonum flaccidum stem, oil
Tea cake, the prevention effect of human urine.
The effect of aphid is on various concentrations polygonum flaccidum stem preventing and treating fresh kidney beans：The 40-42 aphid death rate is handled without significant difference,
But processing 40-42 be significantly higher than processing 43-48 and CK4, i.e., polygonum flaccidum stem preventing and treating fresh kidney beans on aphid effect be significantly better than Oil-tea-cake,
The prevention effect of human urine, but not as good as the prevention effect of tripterygium root.The effect of aphid is on various concentrations Oil-tea-cake preventing and treating fresh kidney beans：
The aphid death rate of processing 45 is significantly higher than processing 43, illustrates that the effect of aphid on higher concentration Oil-tea-cake preventing and treating fresh kidney beans is significantly excellent
In the Oil-tea-cake of low concentration, but not as good as the prevention effect of tripterygium root and polygonum flaccidum stem.Aphid on various concentrations human urine preventing and treating fresh kidney beans
The effect of worm is：Handle effects of the 46-48 and CK4 without aphid on significant difference, i.e. human urine preventing and treating fresh kidney beans not notable.
Shown by above single group separating tests, higher concentration tripterygium root preventing and treating Chinese cabbage, cucumber, radish, the aphid on fresh kidney beans
Its effect is notable.With the reduction of concentration, prevention effect is remarkably decreased.Secondly its prevention effect is followed successively by polygonum flaccidum stem, Oil-tea-cake.
Human urine individually is used, its prevention effect is not notable.With the reduction of concentration, prevention effect is remarkably decreased.Single group separating tests are also shown that
Each component is used alone, and its prevention effect is below 50%.
Compatibility mechanism
Compatibility mechanism is mainly qualitative and quantitative and determines the effect test that each ingredients is prevented and treated organic vegetable aphid.
Materials and methods
2.1.1 test material
Experiment vegetable species are from Chinese cabbage, cucumber, the four kinds of vegetables of radish and fresh kidney beans for being easiest to break out aphid.Experiment is with matching somebody with somebody
Agent is：Tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake.
Test method
The preparation of test material：Material tripterygium root, polygonum flaccidum stem, Oil-tea-cake, human urine all should be selected and come from soil, water, air
Etc. the free of contamination area of environmental condition, tripterygium root, polygonum flaccidum stem, Oil-tea-cake use fresh and alive material, and human urine is deposited undoped with excrement etc.
Put 1-30d pure urine.Oil-tea-cake is the dry cake of 1-30d moisture absorption of not going mouldy.First by the mud on tripterygium root, polygonum flaccidum stem during preparation
Soil removes, and does not need washing.Thereafter, tripterygium root, polygonum flaccidum stem smash damage with hammer open and remove, be cut into the segment of about 10cm length.With hammer handle
Oil-tea-cake is pounded the fritter of about 5 × 5cm sizes, is soaked in proportion in urine, capping immersion 15-30d after mixing.Tripterygium root,
Polygonum flaccidum stem is basis by fresh, and Oil-tea-cake is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, and human urine is deposited by undoped with excrement etc.
The pure urine weight for putting 1-30d is calculated.Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of the component of Oil-tea-cake four are 3.0~4.0:1.0
~2.0:6.0~8.0:0.5~1.0, tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake by 1-30d moisture absorption of not going mouldy
Dry cake weight is calculated, and human urine is pressed the pure urine weight for depositing 30d undoped with excrement etc. and calculated（Wherein human urine is brewed medium, oil tea
Cake is auxiliary agent）.Capping immersion 15d after mixing.After capping immersion 15d, 4 layers of filtered through gauze 2~3 times collects filtrate, is put into appearance
Device, is sealed immediately, is this organic vegetable control of aphids agent stoste, is preserved 1-6 months.The control of aphids agent of this organic vegetable
Applied before insect pest occurs, prevention is pressed per 667m25~10kg stostes, 10 times of liquid sprayings；Applied after insect pest generation, by every
667m210~15kg stostes, 10 times of liquid sprayings, can comparatively fast kill aphid, prevent insect pest from spreading, moreover it is possible to supplement the nutrition such as nitrogen,
Accelerate to recover.
The component tripterygium root of the above four, polygonum flaccidum stem, human urine, the most preferred mass ratio of Oil-tea-cake are 3.5:1.5:7.5:
0.75, tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, and human urine is pressed
The pure urine weight for depositing 1-30d undoped with excrement etc. is calculated.Capping immersion 15-30d after mixing.After capping immersion 15-30d, 4 layers
Filtered through gauze 2~3 times, collects filtrate, is put into container, seals immediately, is this organic vegetable control of aphids agent stoste, protects
Deposit 1-6 months.This organic vegetable control of aphids agent is applied before insect pest occurs, and prevention is pressed per 667m210 times of 5~10kg stostes
Liquid is sprayed；Applied after insect pest generation, by every 667m210~15kg stostes, 10 times of liquid sprayings, can comparatively fast kill aphid, prevent
Insect pest spreads, moreover it is possible to supplement the nutrition such as nitrogen, accelerates the recovery of plant injury, promotes growth.When breaking out aphid, lid is beaten
Open, stoste is taken out, by every 667m25th, 10,10 times of liquid of 3 concentration gradients of 15kg stostes（Refer to amount of water）Spraying.
Experiment is carried out in Yiyang proving ground.The plot that aphid insect pest is often sent out from former years is carried out.When Chinese cabbage, cucumber, radish
Prevention effect experiment is carried out when being broken out with fresh kidney beans aphid；Control is located at same plot.Test effect mainly uses same vegetables
When aphid is broken out, after spraying medicament, counted and statistical analysis, compareed with the percentage of the aphid death rate（CK）Water spray.Chinese cabbage,
Cucumber, four kinds of vegetables of radish and fresh kidney beans are when aphid is broken out, and selection carries out spray processing in the morning or during the dusk.24h is applied in spraying
Inside do not sprinkle water or apply fertilizer, observed and counted during 24h.Conventional rich water quality management after 24h.Experiment sets 36 to handle 4
Control, each 3 repetitions of processing, each repeatedly 0.10m2, random distribution.Using Percent mortality as treatment effect, tie simultaneously
Close field observation.The proportioning of each ingredients is shown in the following description to each processing with amount of application.
Processing 1：The aphid of Chinese cabbage --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:
0.5 presses per 667m25kg is applied；Processing 2：The aphid of Chinese cabbage --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake
For 3.0:1.0:6.0:0.5 presses per 667m210kg is applied；Processing 3：The aphid of Chinese cabbage --- tripterygium root, polygonum flaccidum stem, human urine,
The mass ratio of Oil-tea-cake is 3.0:1.0:6.0:0.5 presses per 667m215kg is applied；Processing 4：The aphid of Chinese cabbage --- tripterygium wilfordii
Root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 presses per 667m25kg is applied；Processing 5：Chinese cabbage
Aphid --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 presses per 667m210kg is applied
With；Processing 6：The aphid of Chinese cabbage --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75
By every 667m215kg is applied；Processing 7：The aphid of Chinese cabbage --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are
4.0:2.0:8.0:1.0 press per 667m25g is applied；Processing 8：The aphid of Chinese cabbage --- tripterygium root, polygonum flaccidum stem, human urine, oil tea
The mass ratio of cake is 4.0:2.0:8.0:1.0 press per 667m210g is applied；Processing 9：It is the aphid of Chinese cabbage --- tripterygium root, peppery
Knotweed stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 press per 667m215g is applied；CK1：The control of Chinese cabbage, no
Spray pharmacy, water spray.Processing 10：The aphid of cucumber --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.0:
1.0:6.0:0.5 presses per 667m25kg is applied；Processing 11：The aphid of cucumber --- tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake
Mass ratio be 3.0:1.0:6.0:0.5 presses per 667m210kg is applied；Processing 12：It is the aphid of cucumber --- tripterygium root, peppery
Knotweed stem, human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 presses per 667m215kg is applied；Processing 13：The aphid of cucumber
Worm --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 presses per 667m25kg is applied；
Processing 14：The aphid of cucumber --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 presses
Per 667m210kg is applied；Processing 15：The aphid of cucumber --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are
3.5:1.5:7.5:0.75 presses per 667m215kg is applied；Processing 16：The aphid of cucumber --- tripterygium root, polygonum flaccidum stem, human urine,
The mass ratio of Oil-tea-cake is 4.0:2.0:8.0:1.0 press per 667m25g is applied；Processing 17：The aphid of cucumber --- tripterygium wilfordii
Root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 press per 667m210g is applied；Processing 18：Cucumber
Aphid --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 press per 667m215g is applied
With；CK2：The control of cucumber, spray pharmacy, does not spray water.Processing 19：The aphid of radish --- tripterygium root, polygonum flaccidum stem, human urine, oil tea
The mass ratio of cake is 3.0:1.0:6.0:0.5 presses per 667m25kg is applied；Processing 20：It is the aphid of radish --- tripterygium root, peppery
Knotweed stem, human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 presses per 667m210kg is applied；Processing 21：The aphid of radish
Worm --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 presses per 667m215kg is applied；
Processing 22：The aphid of radish --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 by every
667m25kg is applied；Processing 23：The aphid of radish --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:
1.5:7.5:0.75 presses per 667m210kg is applied；Processing 24：The aphid of radish --- tripterygium root, polygonum flaccidum stem, human urine, oil tea
The mass ratio of cake is 3.5:1.5:7.5:0.75 presses per 667m215kg is applied；Processing 25：The aphid of radish --- tripterygium root,
Polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 press per 667m25g is applied；Processing 26：The aphid of radish
Worm --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 press per 667m210g is applied；Place
Reason 27：The aphid of radish --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 by every
667m215g is applied；CK3：The control of radish, spray pharmacy, does not spray water.Processing 28：The aphid of fresh kidney beans --- tripterygium root, polygonum flaccidum
Stem, human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 presses per 667m25kg is applied；Processing 29：The aphid of fresh kidney beans
Worm --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 presses per 667m210kg is applied；
Processing 30：The aphid of fresh kidney beans --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 by every
667m215kg is applied；Processing 31：The aphid of fresh kidney beans --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:
1.5:7.5:0.75 presses per 667m25kg is applied；Processing 32：The aphid of fresh kidney beans --- tripterygium root, polygonum flaccidum stem, human urine, oil tea
The mass ratio of cake is 3.5:1.5:7.5:0.75 presses per 667m210kg is applied；Processing 33：The aphid of fresh kidney beans --- tripterygium root,
Polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 presses per 667m215kg is applied；Processing 34：Fresh kidney beans
Aphid --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 press per 667m25g is applied；
Processing 35：The aphid of fresh kidney beans --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 by every
667m210g is applied；Processing 36：The aphid of fresh kidney beans --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:
2.0:8.0:1.0 press per 667m215g is applied；CK4：The control of fresh kidney beans, spray pharmacy, does not spray water.
Statistical analysis
SAS is used to test data（6.12）Carry out statistical analysis.It was found from table 3-4, aphid on processing 1-9 preventing and treating Chinese cabbages
Effect be significantly better than CK1, processing 4-6 effect is significantly better than processing 1-3 again, with handling 7-9 on the whole without significant difference, i.e.,
Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 compatibility is pressed per 667m25-15kg is applied
It is 3.0 that prevention effect, which is significantly better than tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake,:1.0:6.0:0.5 matches somebody with somebody
5 press per 667m2The prevention effect that 5-15kg is applied, i.e. tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.0:
1.0:6.0:0.5 compatibility is pressed per 667m25kg administrations are the lower limits of its compatibility concentration.Tripterygium root, polygonum flaccidum stem, human urine, oil
The mass ratio of tea cake is 3.5:1.5:7.5:The mass ratio of 0.75 compatibility and tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake is
4.0:2.0:8.0:Its prevention effect of 1.0 compatibility is without significant difference, i.e. tripterygium root, polygonum flaccidum stem, human urine, the quality of Oil-tea-cake
Than for 4.0:2.0:8.0:1.0 compatibility is pressed per 667m215kg administrations are the upper limits of its compatibility concentration.Imitated from cost with preventing and treating
Really 2 aspects are accounted for, and tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 matches somebody with somebody
5, its is cost-effective, and raw material, usage amount are moderate, and prevention effect is again good, is preferable optimization formula ratio and amount of application.
Chinese cabbage during 3. ingredients test process 24h of table, the aphid death rate on cucumber（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 1 | 69.25±5.07c | Processing 10 | 65.28±3.27d |
| Processing 2 | 70.61±3.09c | Processing 11 | 76.35±3.62cd |
| Processing 3 | 78.32±0.71bc | Processing 12 | 80.14±1.49cd |
| Processing 4 | 82.36±2.70b | Processing 13 | 84.60±2.18c |
| Processing 5 | 90.13±1.82ab | Processing 14 | 92.16±3.30ab |
| Processing 6 | 95.30±4.16a | Processing 15 | 96.34±4.21a |
| Processing 7 | 84.20±1.80b | Processing 16 | 90.27±0.59ab |
| Processing 8 | 93.07±2.58ab | Processing 17 | 96.43±3.10a |
| Processing 9 | 98.16±3.55a | Processing 18 | 98.10±1.91a |
| CK1 | 3.03±1.27d | CK2 | 2.15±0.60e |
The effect of aphid is significantly better than CK2 on processing 10-18 preventing and treating cucumber, and processing 13-15 effect is significantly better than place again
10-12 is managed, the mass ratio with processing 16-18 on the whole without significant difference, i.e. tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake is
3.5:1.5:7.5:0.75 compatibility is pressed per 667m25-15kg apply prevention effect be significantly better than tripterygium root, polygonum flaccidum stem,
Human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 compatibility is pressed per 667m2The prevention effect that 5-15kg is applied, i.e. thunder
Public rattan root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 compatibility is pressed per 667m2It is it that 5kg, which is applied,
The lower limit of compatibility concentration.Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 compatibility with
Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:Its prevention effect of 1.0 compatibility is without significance difference
Different, i.e., tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 compatibility is pressed per 667m2 15kg
Using the upper limit for being its compatibility concentration.Accounted in terms of cost and prevention effect 2, tripterygium root, polygonum flaccidum stem, human urine,
The mass ratio of Oil-tea-cake is 3.5:1.5:7.5:0.75 compatibility, its is cost-effective, and raw material, usage amount are moderate, and preventing and treating effect
Fruit is again good, is preferable optimization formula ratio and amount of application.
The effect of aphid is significantly better than CK3 on processing 19-27 preventing and treating radish, and processing 22-24 effect is significantly better than processing again
19-21, the mass ratio with processing 25-27 on the whole without significant difference, i.e. tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake is 3.5:
1.5:7.5:0.75 compatibility is pressed per 667m25-15kg apply prevention effect be significantly better than tripterygium root, polygonum flaccidum stem, human urine,
The mass ratio of Oil-tea-cake is 3.0:1.0:6.0:0.5 compatibility is pressed per 667m2The prevention effect that 5-15kg is applied, i.e. tripterygium wilfordii
Root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 compatibility is pressed per 667m2It is its compatibility that 5kg, which is applied,
The lower limit of concentration.Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 compatibility and Thunder God
Rattan root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:Its prevention effect of 1.0 compatibility is without significant difference, i.e.,
Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 compatibility is pressed per 667m215kg is applied
It is the upper limit of its compatibility concentration.Accounted in terms of cost and prevention effect 2, tripterygium root, polygonum flaccidum stem, human urine, oil tea
The mass ratio of cake is 3.5:1.5:7.5:0.75 compatibility, its is cost-effective, and raw material, usage amount are moderate, and prevention effect is again
It is good, it is preferable optimization formula ratio and amount of application.
Radish during 4. ingredients test process 24h of table, the aphid death rate on fresh kidney beans（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 19 | 60.22±2.64e | Processing 28 | 60.16±0.91c |
| Processing 20 | 73.15±0.81d | Processing 29 | 78.59±3.04bc |
| Processing 21 | 81.53±2.19c | Processing 30 | 83.47±1.22b |
| Processing 22 | 89.80±1.02b | Processing 31 | 85.09±2.60b |
| Processing 23 | 92.41±0.53ab | Processing 32 | 94.20±3.59a |
| Processing 24 | 96.38±4.27a | Processing 33 | 96.76±0.58a |
| Processing 25 | 88.09±1.93b | Processing 34 | 92.33±4.06ab |
| Processing 26 | 96.84±0.61a | Processing 35 | 96.18±0.83a |
| Processing 27 | 98.46±2.49a | Processing 36 | 98.70±1.31a |
| CK3 | 1.10±0.53f | CK4 | 3.25±1.24d |
The effect of aphid is significantly better than CK4 on processing 28-36 preventing and treating fresh kidney beans, and processing 31-33 effect is significantly better than place again
28-30 is managed, the mass ratio with processing 34-36 on the whole without significant difference, i.e. tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake is
3.5:1.5:7.5:0.75 compatibility is pressed per 667m25-15kg apply prevention effect be significantly better than tripterygium root, polygonum flaccidum stem,
Human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 compatibility is pressed per 667m2The prevention effect that 5-15kg is applied, i.e. thunder
Public rattan root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.0:1.0:6.0:0.5 compatibility is pressed per 667m2It is it that 5kg, which is applied,
The lower limit of compatibility concentration.Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 compatibility with
Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:Its prevention effect of 1.0 compatibility is without significance difference
Different, i.e., tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 4.0:2.0:8.0:1.0 compatibility is pressed per 667m2 15kg
Using the upper limit for being its compatibility concentration.Accounted in terms of cost and prevention effect 2, tripterygium root, polygonum flaccidum stem, human urine,
The mass ratio of Oil-tea-cake is 3.5:1.5:7.5:0.75 compatibility, its is cost-effective, and raw material, usage amount are moderate, and preventing and treating effect
Fruit is again good, is preferable optimization formula ratio and amount of application.
More than to Chinese cabbage, cucumber, the experiment of four kinds of vegetable aphids of radish and fresh kidney beans it was found from, tripterygium root, polygonum flaccidum stem, people
Urine, the mass ratio of Oil-tea-cake are 3.0~4.0:1.0~2.0:6.0~8.0:0.5~1.0 presses per 667m25-15kg is applied
The upper limit and lower limit of its prevention effect.Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75
By every 667m25kg is applied, and its is cost-effective, and raw material, usage amount are moderate, and prevention effect is again good, is preferably preferably to match somebody with somebody
Side's ratio and amount of application.It is same to press per 667m it was found from table 1-2 and table 3-4 comparative analysis2The preventing and treating effect of 5-15kg amounts of application
Really, its table 3-4 prevention effect is significantly better than table 1-2 prevention effect, i.e. the prevention effect of ingredients is significantly better than the anti-of single dose
Control effect；And the prevention effect of ingredients is not equal to the summation of the prevention effect of each single dose of composition ingredients, the prevention effect of ingredients
More than the summation of the prevention effect of each single dose of composition ingredients, i.e. tripterygium root, polygonum flaccidum stem, human urine, the compatibility of Oil-tea-cake has
Act synergistically, that is, the prevention effect after its compatibility has synergistic effect.
Auxiliary agent is tested
Auxiliary agent experiment is mainly qualitative and quantitative and determines influence of the auxiliary agent to the prevention effect of whole ingredients.
Materials and methods
3.1.1 test material
Experiment vegetable species are Chinese cabbage, cucumber, four kinds of vegetables of radish and fresh kidney beans.Experiment is tripterygium root, polygonum flaccidum with ingredients
Stem, human urine, auxiliary agent are Oil-tea-cake.
Test method
Experiment is carried out in Yiyang proving ground.The plot that aphid insect pest is often sent out from former years is carried out.When Chinese cabbage, cucumber, radish
Prevention effect experiment is carried out when being broken out with fresh kidney beans aphid；Control is located at same plot.Test effect mainly uses same vegetables
When aphid is broken out, after spraying medicament, counted and statistical analysis, compareed with the percentage of the aphid death rate（CK）Water spray.Everywhere
Medicament is managed by 10 times of liquid（Refer to amount of water）Sprayed with sprayer.
Chinese cabbage, cucumber, four kinds of vegetables of radish and fresh kidney beans are when aphid is broken out, and selection is carried out at spray in the morning or during the dusk
Reason.Spraying, which is applied, does not sprinkle water or applies fertilizer in 24h, is observed and is counted during 24h.Conventional rich water quality management after 24h.Experiment is set
Put 12 processing, 4 controls, each 3 repetitions of processing, each repeatedly 0.10m2, random distribution.Place is used as using Percent mortality
Effect is managed, in combination with field observation.Tripterygium root, polygonum flaccidum stem, human urine, the preparation of Oil-tea-cake and brewing method are ibid（With
2.1.2 it is same）, proportioning and the amount of application of each ingredients are shown in the following description to each processing.
Processing 1：The aphid of Chinese cabbage --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:
0.75 presses per 667m210kg is sprayed；Processing 2：The aphid of Chinese cabbage --- tripterygium root, polygonum flaccidum stem, the mass ratio of human urine are 3.5:
1.5:7.5 press per 667m210kg is sprayed；Processing 3：The aphid of Chinese cabbage --- human urine, the mass ratio of Oil-tea-cake are 7.5:0.75 presses
Per 667m210kg is sprayed；CK1：The control of Chinese cabbage, spray pharmacy, does not spray water.Processing 4：It is the aphid of cucumber --- tripterygium root, peppery
Knotweed stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 presses per 667m210kg is sprayed；Processing 5：The aphid of cucumber
Worm --- tripterygium root, polygonum flaccidum stem, the mass ratio of human urine are 3.5:1.5:7.5 press per 667m210kg is sprayed；Processing 6：Cucumber
Aphid --- human urine, Oil-tea-cake mass ratio be 7.5:0.75 presses per 667m210kg is sprayed；CK2：The control of cucumber, does not spray
Medicament, water spray.Processing 7：The aphid of radish --- tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:
7.5:0.75 presses per 667m210kg is sprayed；Processing 8：The aphid of radish --- tripterygium root, polygonum flaccidum stem, the mass ratio of human urine are
3.5:1.5:7.5 press per 667m210kg is sprayed；Processing 9：The aphid of radish --- human urine, the mass ratio of Oil-tea-cake are 7.5:
0.75 presses per 667m210kg is sprayed；CK3：The control of radish, spray pharmacy, does not spray water.Processing 10：The aphid of fresh kidney beans --- Thunder God
Rattan root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:0.75 presses per 667m210kg is sprayed；Processing 11：Beans
The aphid at angle --- tripterygium root, polygonum flaccidum stem, the mass ratio of human urine are 3.5:1.5:7.5 press per 667m210kg is sprayed；Processing
12：The aphid of fresh kidney beans --- human urine, the mass ratio of Oil-tea-cake are 7.5:0.75 presses per 667m210kg is sprayed；CK4：Pair of fresh kidney beans
According to spray pharmacy, does not spray water.
Statistical analysis
SAS is used to test data（6.12）Carry out statistical analysis.As known from Table 5, processing 1 prevents and treats the effect of aphid on Chinese cabbage
Fruit is significantly better than CK1 and processing 2-3, and the effect of processing 2 is significantly better than processing 3, i.e. tripterygium root, polygonum flaccidum stem, human urine, oil tea again
The mass ratio of cake is 3.5:1.5:7.5:The ingredients of 0.75 compatibility is pressed per 667m2The prevention effect that 10kg is sprayed is best, is secondly
Tripterygium root, polygonum flaccidum stem, the mass ratio of human urine are 3.5:1.5:The prevention effect of the ingredients of 7.5 compatibilities is again human urine, oil tea
The mass ratio of cake is 7.5:0.75 presses per 667m2The prevention effect that 10kg is sprayed.Experiment shows that only auxiliary agent Oil-tea-cake is anti-
Control the prevention effect that effect handles 1-2 far away from ingredients；The prevention effect of the processing 2 of ingredients without auxiliary agent is again far away from there is auxiliary agent
The prevention effect of the processing 1 of ingredients.Thus illustrate, auxiliary agent Oil-tea-cake is for aphid to have significantly on preventing and treating Chinese cabbage in this ingredients
Synergistic effect.
Chinese cabbage during 5. auxiliary agent test process 24h of table, the aphid death rate on cucumber（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 1 | 90.13±3.51a | Processing 4 | 92.16±1.15a |
| Processing 2 | 72.84±8.24b | Processing 5 | 67.90±3.56b |
| Processing 3 | 4.26±1.65c | Processing 6 | 3.54±0.49c |
| CK1 | 3.03±0.49c | CK2 | 2.15±0.38c |
The effect that processing 4 prevents and treats aphid on cucumber is significantly better than CK2 and processing 5-6, and the effect of processing 5 is significantly better than place again
Reason 6, i.e. tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of Oil-tea-cake are 3.5:1.5:7.5:The ingredients of 0.75 compatibility is by every
667m2Prevention effect that 10kg is sprayed secondly is that tripterygium root, polygonum flaccidum stem, the mass ratio of human urine are 3.5 preferably:1.5:7.5
The prevention effect of the ingredients of compatibility is again human urine, the mass ratio of Oil-tea-cake is 7.5:0.75 presses per 667m2What 10kg was sprayed
Prevention effect.Experiment shows that only the prevention effect of auxiliary agent Oil-tea-cake handles 4-5 prevention effect far away from ingredients；Without auxiliary agent
Ingredients processing 5 prevention effect again far away from the processing 4 for having auxiliary agent ingredients prevention effect.Thus illustrate, auxiliary agent oil tea
Cake is for aphid to have significant synergies on preventing and treating cucumber in this ingredients.
As known from Table 6, the effect of aphid is significantly better than CK3 and processing 8-9 on the preventing and treating of processing 7 radish, and the effect of processing 8 is again
Be significantly better than processing 9, i.e. tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake mass ratio be 3.5:1.5:7.5:0.75 compatibility is matched somebody with somebody
Agent is pressed per 667m2Prevention effect that 10kg is sprayed secondly is that tripterygium root, polygonum flaccidum stem, the mass ratio of human urine are 3.5 preferably:
1.5:The prevention effect of the ingredients of 7.5 compatibilities is again human urine, the mass ratio of Oil-tea-cake is 7.5:0.75 presses per 667m2 10kg
The prevention effect sprayed.Experiment shows that only the prevention effect of auxiliary agent Oil-tea-cake handles 7-8 prevention effect far away from ingredients；
The prevention effect of the processing 8 of ingredients without auxiliary agent is again far away from the prevention effect for the processing 7 for having auxiliary agent ingredients.Thus illustrate, help
Agent Oil-tea-cake is for aphid to have significant synergies on preventing and treating radish in this ingredients.
Radish during 6. auxiliary agent test process 24h of table, the aphid death rate on fresh kidney beans（Unit：%）
| Processing | 24h | Processing | 24h |
| Processing 7 | 92.41±1.91a | Processing 10 | 94.20±4.83a |
| Processing 8 | 75.80±3.80b | Processing 11 | 80.14±0.91b |
| Processing 9 | 3.61±1.07c | Processing 12 | 3.17±0.68c |
| CK3 | 1.10±0.61c | CK4 | 3.25±1.35c |
The effect that processing 10 prevents and treats aphid on fresh kidney beans is significantly better than CK4 and processing 11-12, and the effect of processing 11 is again significantly excellent
In handle 12, i.e. tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake mass ratio be 3.5:1.5:7.5:The ingredients of 0.75 compatibility is pressed
Per 667m2Prevention effect that 10kg is sprayed secondly is that tripterygium root, polygonum flaccidum stem, the mass ratio of human urine are 3.5 preferably:1.5:
The prevention effect of the ingredients of 7.5 compatibilities is again human urine, the mass ratio of Oil-tea-cake is 7.5:0.75 presses per 667m210kg is sprayed
Prevention effect.Experiment shows that only the prevention effect of auxiliary agent Oil-tea-cake handles 10-11 prevention effect far away from ingredients；Nothing
The prevention effect of the processing 11 of the ingredients of auxiliary agent is again far away from the prevention effect for the processing 10 for having auxiliary agent ingredients.Thus illustrate, help
Agent Oil-tea-cake is for aphid to have significant synergies on preventing and treating fresh kidney beans in this ingredients.
Experiment shows above（Table 5-6）, significantly, the effect of adding assistant is notable for the difference on effect of adding assistant and not adding assistant
Better than the effect of not adding assistant, its effect of adding assistant is not significantly reduced.Human urine is mainly used as brewed medium, while utilizing its fertilizer efficiency
The recovery after insect pest can be effectively facilitated, equivalent to while having carried out a foliage dressing after spray processing.Experiment also shows（Table 1-
6）, all it is significantly higher than organic vegetable control of aphids tripterygium root, polygonum flaccidum stem, compatibility whether effect CK；The effect of used additives
Fruit is not significantly different with CK；Auxiliary agent Oil-tea-cake, tripterygium root, polygonum flaccidum stem, the mass ratio of human urine are 0.75:3.5:1.5:7.5
The ingredients of compatibility is pressed per 667m2Preferably, its compatibility is optimal compatibility to the prevention effect that 10kg is sprayed（Table 1-6）.Experiment shows,
After 15d, its main functional component wilfordine, general flavone, Tea Saponin etc. are not detected, during which do not descend moderate rain or heavy rain；Using
Afterwards, during which following moderate rain or heavy rain more than 1 time, after 10d, its main functional component wilfordine, general flavone, Tea Saponin etc. are not
Detection.Tripterygium wilfordii（Tripterygium wilfordii Hook. f.）, Celastraceae, tripterygium plant.Be also known as herba fibraureae recisae,
Yellow cured rattan etc..Liana shrub, main product is in China, Korea, Japan, and China is distributed mainly on various regions and southwest ground on the south the Yangtze river basin
Area, main product is in Fujian, Zhejiang, Anhui, Henan and other places.It is born in in the shade how wet hillside, mountain valley, small stream side bushes.Happiness is more
Shady and cool hillside, is most preferably grown with the deep sand soil of slant acidity, soil layer or yellow soil.Bitter, pungent, cool in nature, big poison.Tripterygium wilfordii
Belong to liana shrub, high 1-3 meters, sprig brownish red, with the thin rib of 4-6 roots, by Mi Mao and fine and closely woven hole skin.Elliptic leaf shape, the ovum that falls are ellipse
Circular, rectangular oval or avette, long 4-7.5 centimetres, wide 3-4 centimetres, tip is suddenly sharp or short tapering, base portion wealthy wedge shape or circle
There is a serration at shape, edge, 4-7 pairs of lateral vein, up to leaf margin after it is slightly upper curved；Petiole is long 5-8 millimeters, close by rust hair.The poly- umbrella flower of circular cone
Sequence is narrower, long 5-7 centimetres, wide 3-4 centimetres, generally there is 3-5 branches.The long round shape of samara, long 1-1.5 centimetres, 1-1.2 lis of diameter
Rice, seed buttress shaft shape, up to 10 millimeters.Tripterygium wilfordii bitter, pungent, cool in nature, big poison, live with clearing heat and detoxicating, dispelling wind and removing obstruction in the meridians, relaxing muscles and tendons
The effects such as blood, swelling and pain relieving, desinsection stop blooding, returns liver and kidney channel, effect dispelling wind and eliminating dampness, remove obstruction in channels to relieve pain, swelling and pain relieving, solution poisoning
Worm.The traditional Chinese medical science is used to treat lepra reaction, rheumatoid arthritis, pulmonary tuberculosis and other chronic lung diseases etc..Tripterygium wilfordii has anti-
Tumour, anti-inflammatory etc. are acted on, but its is very toxic, so tripterygium wilfordii is constantly subjected to limitation in application medically.Tripterygium root is main
Containing wilfordine（wilfordine）, celastrus orbiculatus benzyl acid amides（celagbenzine）, Triptolide（wilforlide）Deng.It is peppery
Knotweed（Polygonum flaccidum Meissn. ）It is polygonaceae Polygonum, alias：The burnt grass of class, flaccid knotweed herb etc., annual herb is planted
Thing, high 60~90cm, complete stool spreads gland point and young pilose antler.Stem is upright, or bottom is thrown oneself on the ground, and usual aubergine, section expands, leaf alternate,
There is short handle.The wide lanceolar of blade, tip is tapering, base portion wedge shape, two sides by coarse wool, above bottle green, have splay blackspot, hold in the palm
Leaf sheath film quality, edge growth seta.Spike is born in Zhi Ding, and bennet is elongated, long 6~12cm, sagging, flower thinning；The drastic crack of perianth 5,
White, spreads green gland point, top takes on a red color；Stamen 7~8；The Room of ovary 1,3 pieces of style.The rib of achene 3, outsourcing persitent perianth.Flower
Summer phase.Florescence summer, aciculiform blade is to have splayed blackspot, and flower is born in Zhi Ding for spike sequence.National most area has
Distribution, is born in nearly waterside, dark and damp place.Polygonum flaccidum has expelling wind and removing dampness, blood stasis removing analgesic, removing toxicity for detumescence, the effect of desinsection is antipruritic, the traditional Chinese medical science
For treating dysentery, gastroenteritis, diarrhoea, rheumatic arthritis, treating swelling and pain by traumatic injury, functional uterine bleeding；Venomous snake bite, skin are controlled in external application
Skin eczema etc..It is the polygonum flaccidum herb rutin of monomer containing flavones (Rutin), Hyperoside (Hyperin), quercetin (Quercetin), different
Rhamnetin (Isorhamnetin) and kaempferol (Kaempferol) and volatile oil n-decane, a small amount of nopinene, limonene and just
Hendecane etc. is poisonous.Oil tea（Camellia oleifera Abel.）Belong to Theaceae, Camellia, alias：Ribes tree, tea oil tree
Deng.Evergreen dungarunga.Because its seed can extract oil（Tea oil）It is edible, it is therefore named.Shrub or middle arbor；Spray has coarse wool.Leaf keratin,
Ellipse, Long Circle or obovate, tip is sharp and has blunt nosed, and sometimes tapering or blunt, base portion wedge shape is long 5-7 centimetres, wide 2-4 lis
Rice, it is sometimes longer, above bottle green, shinny, middle arteries have coarse wool or pubescence, below light green color, hairless or middle arteries become mildewed, lateral vein
It can see below not it is obvious that there is serration at edge, have cognate sometimes, petiole is long 4-8 millimeters, there is coarse wool above.Tea oil tree
It is one of big woody oleiferous plants in the world four, it is grown in high mountain and the hilly country of southern china subtropical zone, is that China is peculiar
A kind of pure natural senior oil plant.China cultivates extensively from the Yangtze river basin to south China various regions.It is concentrated mainly on Zhejiang, Jiangxi, river
South, Hunan, Guang Xiwu are saved.Tea oil color clear flavor incense, nutritious, storage tolerance is high quality food oil；Also can be as lubricating oil, anti-
Rust oil is used for industry.Tea cake is both agricultural chemicals, is fertilizer again, can improve farmland water-holding capacity and preventing and treating rice pest.In leached tea oil slag
Containing Tea Saponin, tea seed polysaccharide, tea seed albumen etc., they are all the raw materials of chemical industry, light industry, food, feed industry product etc..Urine
Liquid：Urine 95% or so is water, and remaining is solid portion.In solids, inorganic substances have sodium, potassium, chlorine, calcium, phosphorus, magnesium and sulfuric acid
The inorganic salts such as salt, phosphate, ammonium salt；Organic substance mainly have urea, uric acid, hippuric acid, creatine and creatinine, amino acid and
Ammonia, urokinase, EGF, growth hormone, erythropoietin, promoting sexual gland hormone, callicrein and antitumor
Peptide matters etc..So, tripterygium root, polygonum flaccidum stem, Oil-tea-cake are immersed in urine, inorganic salts, the organic principle energy of urine
Effectively inorganic, the organic principle of tripterygium root, polygonum flaccidum stem, Oil-tea-cake are dissolved in urine, naturally extracted purpose is reached.
Experiment shows that main functional component wilfordine of tripterygium root etc. has good stomach poison function, polygonum flaccidum stem
The functional components such as general flavone+volatile oil n-decane not only have good action of contace poison, moreover it is possible to promote the functions such as wilfordine into
Divide the speed acted in vegetable aphid toxicity in vivo, the two has collaboration, synergistic effect well.Tripterygium root, polygonum flaccidum stem
Main functional component have to vegetable aphid it is good tag, interior suction, the toxic action such as stomach toxicity.The functions such as the Tea Saponin of Oil-tea-cake
Composition not only can effectively dissolve the functional components such as wilfordine, general flavone, significantly improve to the tagging of vegetable aphid, interior suction, stomach
The toxic action of poison etc., Tea Saponin etc. can also form soap-film, block the stomata of aphid, enhance tripterygium root, the poison of polygonum flaccidum stem
The effect of killing, is a kind of good auxiliary agent.
Embodiment
Implementation below and embodiment are intended to further illustrate the present invention, rather than limitation of the present invention.
The preparation of control agent stoste：Tripterygium root, polygonum flaccidum stem, Oil-tea-cake, human urine all should be selected from soil, water, air etc.
The free of contamination area of environmental condition, tripterygium root, polygonum flaccidum stem, Oil-tea-cake use fresh and alive material, and human urine is deposited undoped with excrement etc.
1-30d pure urine.Oil-tea-cake is the dry cake of 1-30d moisture absorption of not going mouldy.First by the soil on tripterygium root, polygonum flaccidum stem during preparation
Remove, do not need washing.Then, tripterygium root, polygonum flaccidum stem smash damage with hammer open and remove, be cut into the segment of about 10cm length.With hammer oil
Tea cake is pounded the fritter of about 5 × 5cm sizes, is soaked in proportion in urine, capping immersion 15d after mixing.Tripterygium root, polygonum flaccidum
Stem, human urine, the mass ratio of the component of Oil-tea-cake four are 3.0~4.0:1.0~2.0:6.0~8.0:0.5~1.0,（Wherein human urine is
Brewed medium, Oil-tea-cake is auxiliary agent）.Tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake by 1-30d moisture absorption of not going mouldy
Dry cake weight is calculated, and human urine is pressed the pure urine weight for depositing 30d undoped with excrement etc. and calculated.Capping immersion 15d after mixing.Capping leaching
Steep after 15d, 4 layers of filtered through gauze 2~3 times collects filtrate, be put into container, seal immediately, be that this organic vegetable aphid prevents
Agent stoste is controlled, is preserved 1-6 months.Applied after insect pest generation, by every 667m25~15kg stostes, 10 times of liquid sprayings, can be very fast
Aphid is killed, prevents insect pest from spreading, moreover it is possible to the nutrition such as nitrogen are supplemented, accelerates to recover.
The component tripterygium root of the above four, polygonum flaccidum stem, human urine, the most preferred mass ratio of Oil-tea-cake are 3.5:1.5:7.5:
0.75, when breaking out aphid, lid is opened, stoste is taken out, by every 667m25~15kg stostes, 10 times of liquid（Refer to amount of water）Spray
Mist.Embodiment is implemented in Yiyang proving ground, and the plot that aphid insect pest is often sent out from former years is carried out.When Chinese cabbage, cucumber, trailing plants
Fore-telling is prevented and treated when being broken out with fresh kidney beans aphid；Control is located at same plot.When effect is mainly broken out with same vegetable aphid,
After spraying medicament, counted and statistical analysis, compareed with the percentage of the aphid death rate（CK）Water spray.Chinese cabbage, cucumber, radish with
Four kinds of vegetables of fresh kidney beans are when aphid is broken out, and selection carries out spray processing in the morning or during the dusk.Spraying apply 24h in do not sprinkle water or
Fertilising, is observed and is counted during 24h.Conventional rich water quality management after 24h.
Embodiment 1：
Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of the component of Oil-tea-cake four are 3.0:1.0:6.0:0.5 presses per 667m2
The prevention effect of 10 times of liquid sprayings of 5kg control agents stoste：
The preparation of control agent stoste：Tripterygium root, polygonum flaccidum stem, Oil-tea-cake, human urine all should be selected from soil, water, air etc.
The free of contamination area of environmental condition, tripterygium root, polygonum flaccidum stem, Oil-tea-cake use fresh and alive material, and human urine is deposited undoped with excrement etc.
1-30d pure urine.Oil-tea-cake is the dry cake of 1-30d moisture absorption of not going mouldy.First by the soil on tripterygium root, polygonum flaccidum stem during preparation
Remove, do not need washing.Thereafter, tripterygium root, polygonum flaccidum stem smash damage with hammer open and remove, be cut into the segment of about 10cm length.With hammer oil
Tea cake is pounded the fritter of about 5 × 5cm sizes, is soaked in proportion in urine, capping immersion 15d after mixing.Tripterygium root, polygonum flaccidum
Stem, human urine, the mass ratio of the component of Oil-tea-cake four are 3.0:1.0:6.0:0.5,（Wherein human urine is brewed medium, and Oil-tea-cake is to help
Agent）.Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, and human urine is pressed
The pure urine weight for depositing 30d undoped with excrement etc. is calculated.Capping immersion 15d after mixing.After capping immersion 15d, 4 layers of filtered through gauze
2~3 times, filtrate is collected, container is put into, seals immediately, be this organic vegetable control of aphids agent stoste, preserve 1-6
Month.Applied after insect pest generation, by every 667m2The 10 times of liquid sprayings of 5kg stostes, can comparatively fast kill aphid, prevent insect pest from spreading,
The nutrition such as nitrogen can also be supplemented, accelerate to recover.Carried out in Yiyang proving ground.The plot for often sending out aphid insect pest from former years is entered
OK.Prevention effect experiment is carried out when Chinese cabbage, cucumber, radish and fresh kidney beans aphid are broken out；Control is located at same plot.Effect is main
It is when being broken out with same vegetable aphid, after spraying medicament, to be counted with the percentage of the aphid death rate and statistical analysis, control
（CK）Water spray.Chinese cabbage, cucumber, four kinds of vegetables of radish and fresh kidney beans are when aphid is broken out, and selection is carried out at spray in the morning or during the dusk
Reason.Spraying, which is applied, does not sprinkle water or applies fertilizer in 24h, is observed and is counted during 24h.Conventional rich water quality management after 24h.Processing
During 24h, the percentage of the aphid death rate is：
Chinese cabbage is that 69.25%, CK is 3.03%, and the aphid death rate is higher than CK 22.85 times；Cucumber is that 65.28%, CK is
2.15%, the aphid death rate is higher than CK 30.36 times；Radish is that 60.22%, CK is 1.10%, and the aphid death rate is higher than CK 54.75 times；
Fresh kidney beans are that 60.16%, CK is 2.15%, and the aphid death rate is higher than CK 27.98 times.
Result of implementation shows, organic vegetable control of aphids agent tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake compatibility mass ratio
For 3.0:1.0:6.0:0.5 presses per 667m2The prevention effect of 10 times of liquid sprayings of 5kg control agents stoste is notable.
Embodiment 2：
Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of the component of Oil-tea-cake four are 3.5:1.5:7.5:0.75 by every
667m2The prevention effect of 10 times of liquid sprayings of 10kg control agents stoste：
The preparation of control agent stoste：Tripterygium root, polygonum flaccidum stem, Oil-tea-cake, human urine all should be selected from soil, water, air etc.
The free of contamination area of environmental condition, tripterygium root, polygonum flaccidum stem, Oil-tea-cake use fresh and alive material, and human urine is deposited undoped with excrement etc.
1-30d pure urine.Oil-tea-cake is the dry cake of 1-30d moisture absorption of not going mouldy.First by the soil on tripterygium root, polygonum flaccidum stem during preparation
Remove, do not need washing.Thereafter, tripterygium root, polygonum flaccidum stem smash damage with hammer open and remove, be cut into the segment of about 10cm length.With hammer oil
Tea cake is pounded the fritter of about 5 × 5cm sizes, is soaked in proportion in urine, capping immersion 15d after mixing.Tripterygium root, polygonum flaccidum
Stem, human urine, the mass ratio of the component of Oil-tea-cake four are 3.5:1.5:7.5:0.75,（Wherein human urine is brewed medium, and Oil-tea-cake is to help
Agent）.Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, and human urine is pressed
The pure urine weight for depositing 30d undoped with excrement etc. is calculated.Capping immersion 15d after mixing.After capping immersion 15d, 4 layers of filtered through gauze
2~3 times, filtrate is collected, container is put into, seals immediately, be this organic vegetable control of aphids agent stoste, preserve 1-6
Month.Applied after insect pest generation, by every 667m2The 10 times of liquid sprayings of 10kg stostes, can comparatively fast kill aphid, prevent insect pest from spreading,
The nutrition such as nitrogen can also be supplemented, accelerate to recover.Carried out in Yiyang proving ground.The plot for often sending out aphid insect pest from former years is entered
OK.Prevention effect experiment is carried out when Chinese cabbage, cucumber, radish and fresh kidney beans aphid are broken out；Control is located at same plot.Effect is main
It is when being broken out with same vegetable aphid, after spraying medicament, to be counted with the percentage of the aphid death rate and statistical analysis, control
（CK）Water spray.Chinese cabbage, cucumber, four kinds of vegetables of radish and fresh kidney beans are when aphid is broken out, and selection is carried out at spray in the morning or during the dusk
Reason.Spraying, which is applied, does not sprinkle water or applies fertilizer in 24h, is observed and is counted during 24h.Conventional rich water quality management after 24h.Processing
During 24h, the percentage of the aphid death rate is：
Chinese cabbage is that 90.13%, CK is 3.03%, and the aphid death rate is higher than CK 29.75 times；Cucumber is that 92.16%, CK is
2.15%, the aphid death rate is higher than CK 42.87 times；Radish is that 92.41%, CK is 1.10%, and the aphid death rate is higher than CK 84.01 times；
Fresh kidney beans are that 94.20%, CK is 2.15%, and the aphid death rate is higher than CK 43.81 times.
Result of implementation shows, organic vegetable control of aphids agent tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake compatibility mass ratio
For 3.5:1.5:7.5:0.75 presses per 667m2The prevention effect of 10 times of liquid sprayings of 10kg control agents stoste is notable.
Embodiment 3：
Tripterygium root, polygonum flaccidum stem, human urine, the mass ratio of the component of Oil-tea-cake four are 4.0:2.0:8.0:1.0 by every
667m2The prevention effect of 10 times of liquid sprayings of 15kg control agents stoste：
The preparation of control agent stoste：Tripterygium root, polygonum flaccidum stem, Oil-tea-cake, human urine all should be selected from soil, water, air etc.
The free of contamination area of environmental condition, tripterygium root, polygonum flaccidum stem, Oil-tea-cake use fresh and alive material, and human urine is deposited undoped with excrement etc.
1-30d pure urine.Oil-tea-cake is the dry cake of 1-30d moisture absorption of not going mouldy.First by the soil on tripterygium root, polygonum flaccidum stem during preparation
Remove, do not need washing.Thereafter, tripterygium root, polygonum flaccidum stem smash damage with hammer open and remove, be cut into the segment of about 10cm length.With hammer oil
Tea cake is pounded the fritter of about 5 × 5cm sizes, is soaked in proportion in urine, capping immersion 15d after mixing.Tripterygium root, polygonum flaccidum
Stem, human urine, the mass ratio of the component of Oil-tea-cake four are 4.0:2.0:8.0:1.0,（Wherein human urine is brewed medium, and Oil-tea-cake is to help
Agent）.Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is calculated by the dry cake weight of 1-30d moisture absorption of not going mouldy, and human urine is pressed
The pure urine weight for depositing 30d undoped with excrement etc. is calculated.Capping immersion 15d after mixing.After capping immersion 15d, 4 layers of filtered through gauze
2~3 times, filtrate is collected, container is put into, seals immediately, be this organic vegetable control of aphids agent stoste, preserve 1-6
Month.Applied after insect pest generation, by every 667m2The 10 times of liquid sprayings of 15kg stostes, can comparatively fast kill aphid, prevent insect pest from spreading,
The nutrition such as nitrogen can also be supplemented, accelerate to recover.Carried out in Yiyang proving ground.The plot for often sending out aphid insect pest from former years is entered
OK.Prevention effect experiment is carried out when Chinese cabbage, cucumber, radish and fresh kidney beans aphid are broken out；Control is located at same plot.Effect is main
It is when being broken out with same vegetable aphid, after spraying medicament, to be counted with the percentage of the aphid death rate and statistical analysis, control
（CK）Water spray.Chinese cabbage, cucumber, four kinds of vegetables of radish and fresh kidney beans are when aphid is broken out, and selection is carried out at spray in the morning or during the dusk
Reason.Spraying, which is applied, does not sprinkle water or applies fertilizer in 24h, is observed and is counted during 24h.Conventional rich water quality management after 24h.Processing
During 24h, the percentage of the aphid death rate is：
Chinese cabbage is that 98.16%, CK is 3.03%, and the aphid death rate is higher than CK 32.40 times；Cucumber is that 98.10%, CK is
2.15%, the aphid death rate is higher than CK 45.63 times；Radish is that 98.46%, CK is 1.10%, and the aphid death rate is higher than CK 89.51 times；
Fresh kidney beans are that 98.70%, CK is 2.15%, and the aphid death rate is higher than CK 45.91 times.
Result of implementation shows, organic vegetable control of aphids control agent tripterygium root, polygonum flaccidum stem, human urine, Oil-tea-cake compatibility matter
Amount is than being 4.0:2.0:8.0:1.0 press per 667m2The prevention effect of 10 times of liquid sprayings of 15kg control agents stoste is notable.
Claims (3)
1. a kind of organic vegetable control of aphids agent, it is characterised in that the organic vegetable control of aphids agent is by tripterygium root, polygonum flaccidum
Stem, human urine and auxiliary agent Oil-tea-cake compatibility is brewed forms, wherein the compatibility quality of tripterygium root, polygonum flaccidum stem, human urine and Oil-tea-cake it
Than for 3.0~4.0:1.0~2.0:6.0~8.0:0.5~1.0, tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake presses 1-
The dry cake weight of 30d moisture absorption of not going mouldy is calculated, and human urine is calculated by pure urine weight of the 1~30d of storage undoped with excrement.
2. a kind of organic vegetable control of aphids agent according to claim 1, it is characterised in that tripterygium root:Polygonum flaccidum stem:People
Urine:The mass ratio of Oil-tea-cake is 3.5:1.5:7.5:0.75.
3. a kind of application method of organic vegetable control of aphids agent as claimed in claim 1, it is characterised in that in vegetable aphid
Applied after generation, by every 667m25~15kg stostes, 10 times of liquid spraying, selection is sprayed in the morning or during the dusk, after
Do not sprinkle water or apply fertilizer in 24h, conventional rich water quality management, if rained using within rear 24h, is then needed again after rain after 24h
Processing.
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| CN101288413A (en) * | 2008-05-26 | 2008-10-22 | 浙江省农业科学院 | Tripterygium wilfordii compound pesticides for high effectively preventing and curing tea geometrid |
| CN102018001A (en) * | 2009-09-14 | 2011-04-20 | 周新荣 | Plant medicament for preventing corn aphids |
| CN104381338A (en) * | 2014-11-10 | 2015-03-04 | 张美丽 | Comphortree root insecticide and preparation method thereof |
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| CN101288413A (en) * | 2008-05-26 | 2008-10-22 | 浙江省农业科学院 | Tripterygium wilfordii compound pesticides for high effectively preventing and curing tea geometrid |
| CN102018001A (en) * | 2009-09-14 | 2011-04-20 | 周新荣 | Plant medicament for preventing corn aphids |
| CN104381338A (en) * | 2014-11-10 | 2015-03-04 | 张美丽 | Comphortree root insecticide and preparation method thereof |
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