CN104904602B - A kind of American aloe rejuvenation culture medium - Google Patents
A kind of American aloe rejuvenation culture medium Download PDFInfo
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- CN104904602B CN104904602B CN201510358873.4A CN201510358873A CN104904602B CN 104904602 B CN104904602 B CN 104904602B CN 201510358873 A CN201510358873 A CN 201510358873A CN 104904602 B CN104904602 B CN 104904602B
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- american aloe
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Abstract
The invention discloses a kind of American aloe rejuvenation culture medium, comprise the raw material of following weight portion: 300～400 parts of organic material of agricultural, the organoclay of 100～150 parts, 30～50 parts of calcareous, 0.5～1 part of zymophytes, 1～5 part of nutritive salt and 0.5～1 part of function bacterium. Organoclay is in fertile soil, peat soil, bentonite, diatomite; Calcareous is the mixture of oyster shell whiting, donkey bone meal; Zymophyte is EM microbial inoculum; Nutritive salt is the mixture of ammonium nitrate, 7 water magnesium sulfates, potassium dihydrogen phosphate, potassium nitrate, inositol, nicotinic acid, thiamine hydrochloride, puridoxine hydrochloride, glycine mixing; Function bacterium comprises phosphate solubilizing bacteria, lactic acid bacteria, saccharomycete, photosynthetic bacteria, nitrogen-fixing bacteria, bacillus subtilis. American aloe rejuvenation culture medium of the present invention, can improve the structure of soil, the nutrients such as manganese in soil, zinc, magnesium, potassium significantly increase, the fungal component of American aloe root system has significant increasing action, the average attack rate of American aloe significantly reduces, be reduced to 15% by original 100%, disease resistance significantly strengthens.
Description
Technical field
The present invention relates to a kind of American aloe rejuvenation culture medium, belong to American aloe cultivation technique field.
Background technology
American aloe (AgaveamericanaL.) has another name called the imperial tongue palm, american aloe, is Agavaceae, AgaveThe large-scale herbaceous plant of perennial evergreen. Originate in american torrid zone, the normal introducing and planting of In South China and southwestern each provinces and regions;Just bloom after generally wanting decades in original producton location, after blooming, maternal plant is withered, and cross-pollination could be solid; In YunnanEscaped raw for many years, and in Red River, the Dry-hot Valley Area in Nujiang, Jinsha jiang River etc. so that Kunming all can normally openBow structure is real.
Agave stem is short, leaf sword shape, trigonometric sum aciculiform etc., and meat, is rosette-stape and arranges, scapeTall and big, spike or panicle top are raw, very beautiful, have ornamental value, are flower gardens, Shelter in South China CitiesAnd the common flowers of greenbelt. But, frostbite, sun burns, the plant excessive growth that causes because of illumination deficiency withAnd mechanical damage etc. causes harmful effect to plant, or the disease harm such as leaf spot, anthracnose and gray moldAmerican aloe growth, makes its butt rot or yellow leaf, and when serious, even whole blade rots, extremely shadowRing ornamental value. At present, for American aloe rejuvenation, turn cultivation or spraying medicine except moving basin, not moreGood rejuvenation method.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, and a kind of dragon is providedThe blue rejuvenation culture medium of tongue, this rejuvenation culture medium not only can have the upgrowth situation of efficient recovery American aloe, all rightImprove the nutritional labeling of American aloe Soil Surrounding, can increase again the beneficial bacterium growth in American aloe soil.
The present invention is achieved through the following technical solutions:
A kind of American aloe rejuvenation culture medium, comprises the raw material of following weight portion: 300～400 parts of organic material of agricultural,The organoclay of 100～150 parts, 30～50 parts of calcareous, 0.5～1 part of zymophytes, 1～5 part of nutritive salt and 0.5～1 partFunction bacterium.
Preferably, the organic material of described agricultural is any one in stalk, bagasse, wood chip, beans bar, ight soilKind, or at least two kinds of mixtures that coordinate with arbitrary proportion.
Preferably, described organoclay is any one in fertile soil, peat soil, bentonite, diatomite,Or at least two kinds of mixtures that coordinate with arbitrary proportion.
Preferably, described calcareous be the mixture of oyster shell whiting, donkey bone meal, the weight of oyster shell whiting, donkey bone mealThan being 2:1.
Preferably, described zymophyte is EM microbial inoculum.
Preferably, described nutritive salt be ammonium nitrate, 7 water magnesium sulfates, potassium dihydrogen phosphate, potassium nitrate, inositol,The mixture that nicotinic acid, thiamine hydrochloride, puridoxine hydrochloride, glycine mix, each components by weight is: 2:1：1：1：1：1：1：1.5：0.5。
Preferably, described function bacterium comprise phosphate solubilizing bacteria, lactic acid bacteria, saccharomycete, photosynthetic bacteria, nitrogen-fixing bacteria,Bacillus subtilis, its weight ratio is 2:1:1:1:1:2.
The present invention also provides a kind of preparation method of above-mentioned American aloe rejuvenation culture medium, comprises the following steps:
(1), agriculture organic material is crushed to 1～2mm; Organoclay is crossed 100～150 mesh sieves, removes large particulate matter,Retain the lower organoclay of sieve; Calcareous specification is, particle diameter 300～500nm; Proportionally take zymophyte and locateThe organic material of agricultural after reason, organoclay and calcareous, by organic agricultural material, organoclay, calcareous proportionally mixedEven;
(2) the organic material of paving one deck agricultural, organoclay, calcareous mixture, spread one deck fermentation uniformly on itBacterium; Repave the organic material of one deck agricultural, organoclay, calcareous mixture, on it, spread uniformly again one deck fermentationBacterium; So stack, stacks into the buttress of high 1 meter, wide 1 meter, long 3 meters; In buttress, every 50 centimetres of intersectionsPut into ventilation duct one deck of 2 cun, place vertically and put a ventilation duct every 1.5 meters, on ventilation duct, have openning hole in advance,Ensure the turnover of sweat air free; Stacking will be loosened, and must not make real the not covering such as handy Polypropylence Sheet;
(3), measure temperature every day, within 3-4 days, compost each point temperature exceedes more than 60 DEG C, turn over throw buttress, then pressStep 2 continues to stack into the buttress of high 1 meter, wide 1 meter, long 3 meters; Continue about 3 days, then turn over throwing once,After turning over throwing, do not continue to heat up, can judge that fermentation completes, can ferment general 14-18 days autumn and winter, the summerWithin season 6-9 days, left and right just can ferment. If in buttress, a some temperature exceedes more than 60 DEG C during twice is turned over throwing,Need spread cold water cooling, must not exceed 70 DEG C, need spray 2-3 cooling every day in summer. While becoming fertile, temperature is generalAt 45 DEG C, moisture should be 30% left and right, as too high in moisture, can divide airing one day. When fermentation, should be placed on and keep awayWind place, is not directly exposed to the sun by sunlight.
(4) product fermenting is mixed and obtains American aloe with nutritive salt, the function bacterium of described ratioRejuvenation culture medium.
The present invention also provides a kind of using method of above-mentioned American aloe rejuvenation culture medium, comprises the following steps:
(1), in advance by organic agricultural material, organoclay, calcareous mixing, add zymophyte and ferment;
(2), after the fermentation material fermenting, be ready to nutritive salt, function bacterium the previous day that applies rejuvenation culture medium;
(2) applied fertilizer the same day, the fermentation material fermenting, nutritive salt, function bacterium were proportionally mixed;
(3) every American aloe root applies fertilizer 2～5 kilograms.
American aloe rejuvenation culture medium of the present invention, can improve the structure of soil, manganese in soil, zinc,The nutrient such as magnesium, potassium significantly increases, and the fungal component of American aloe root system has significant increasing action, imperial tongueBlue average attack rate significantly reduces, and is reduced to 15% by original 100%, and disease resistance significantly strengthens.
Detailed description of the invention
Below in conjunction with embodiment, American aloe rejuvenation culture medium of the present invention is specifically described:
Embodiment 1: preparation American aloe rejuvenation culture medium I
Comprise the following steps:
(1), crushed stalk is to 2mm; Fertile soil is crossed 100 mesh sieves, removes large particulate matter, retains sieve lower rottenGrow soil; Shell, donkey bone meal are broken to 350nm and prepare oyster shell whiting and donkey bone meal; Take 350 parts of powder of straw, 120Part fertile soil, 30 parts of oyster shell whitings, 15 parts of donkey bone meal, 1 portion of EM microbial inoculum, by powder of straw, fertile soil, shellfishShell powder, donkey bone meal proportionally mix and are prepared into mixture;
(2) mixture of paving one deck powder of straw, fertile soil, oyster shell whiting, donkey bone meal, spreads one on it uniformlyLayer zymophyte; Repave one deck mixture, on it, spread uniformly again one deck zymophyte; So stack, stacks intoThe buttress of high 1 meter, wide 1 meter, long 3 meters; In buttress, every 50 centimetres of intersections are put into ventilation duct one deck of 2 cun,Place vertically and put a ventilation duct every 1.5 meters, on ventilation duct, have openning hole in advance, ensure that sweat air free entersGo out; Stacking will be loosened, and must not make real the not covering such as handy Polypropylence Sheet;
(3), measure temperature every day, within 3-4 days, compost each point temperature exceedes more than 60 DEG C, turn over throw buttress, then pressStep 2 continues to stack into the buttress of high 1 meter, wide 1 meter, long 3 meters; Continue about 3 days, then turn over throwing once,After turning over throwing, do not continue to heat up, can judge that fermentation completes, can ferment general 14-18 days autumn and winter, the summerWithin season 6-9 days, left and right just can ferment;
(4) by 0.4 part of ammonium nitrate, 0.2 part of 7 water magnesium sulfate, 0.2 part of potassium dihydrogen phosphate, 0.2 part of potassium nitrate,0.2 part of inositol, 0.2 part of nicotinic acid, 0.2 part of thiamine hydrochloride, 0.3 part of puridoxine hydrochloride, 0.1 glycine mixBe prepared into nutritive salt; By 0.4 part of phosphate solubilizing bacteria, 0.2 part of lactic acid bacteria, 0.2 part of saccharomycete, 0.2 part of photosynthetic bacteria,0.2 part of nitrogen-fixing bacteria is mixed with into function bacterium with 0.4 part of bacillus subtilis;
(5) nutritive salt, function bacterium that fermentation material step 3 being prepared and step 4 prepare are mixed in proportionClose and evenly obtain American aloe rejuvenation culture medium I.
Embodiment 2: preparation American aloe rejuvenation medium ii
In step 1, organoclay is bentonite but not fertile soil, other components and preparation method and embodiment 1 phaseWith.
Embodiment 3: preparation American aloe rejuvenation medium ii I
In step 1, agriculture organic material is beans bar but not stalk, other components and preparation method and embodiment 1 phaseWith.
Comparative example 1: preparation American aloe contrast rejuvenation culture medium A
Do not contain the function bacterium described in the EM microbial inoculum described in embodiment 1 step 1 and step 4, otherComponent and preparation method are identical with embodiment 1.
Comparative example 2: preparation American aloe contrast rejuvenation culture medium B
Do not contain the EM microbial inoculum described in embodiment 1 step 1, other components and preparation method and embodiment 1Identical.
Comparative example 3: preparation American aloe contrast rejuvenation culture medium C
Do not contain the function bacterium described in embodiment 1 step 4, other components and preparation method and embodiment 1Identical.
Demonstration test 1:
The dragon that uses embodiment 1, comparative example 1, comparative example 2, comparative example 3 to prepare separatelyThe blue rejuvenation culture medium of tongue I, contrast rejuvenation culture medium A, contrast rejuvenation culture medium B, contrast rejuvenation culture medium CAmerican aloe is carried out to rejuvenation, 100 every group, while using above-mentioned matrix, every American aloe root 3 public affairs of applying fertilizerJin, rejuvenation, after 1 month, is added up the American aloe incidence of disease, and result is as shown in table 1:
Table 1: American aloe morbidity statistics situation after the rejuvenation of different rejuvenation culture medium
| Project | Contrast rejuvenation culture medium A | Contrast rejuvenation culture medium B | Contrast rejuvenation culture medium C | Rejuvenation culture medium I |
| The incidence of disease before rejuvenation | 100％ | 100％ | 100％ | 100％ |
| Rejuvenation sequela rate | 85％ | 80％ | 82％ | 15％ |
| Cure rate | 15％ | 20％ | 18％ | 85％ |
As shown in Table 1, the incidence of disease before rejuvenation is 100%, and American aloe contrast rejuvenation culture medium A, rightThan rejuvenation culture medium B, contrast rejuvenation culture medium C, American aloe is carried out to rejuvenation, although American aloe can rejuvenation,But have little effect, average attack rate is 82.3%; And American aloe rejuvenation culture medium I of the present invention enters American aloeRow rejuvenation, average attack rate is only 15%, has significantly reduced the incidence of disease of American aloe, American aloe is had aobviousThe rejuvenation effect of work.
Demonstration test 2:
Use rejuvenation culture medium I, contrast rejuvenation culture medium A, contrast rejuvenation culture medium B, contrast rejuvenation to cultivateBase C carries out rejuvenation to American aloe, and 100 every group, while using above-mentioned matrix, every American aloe root fertilising 3Kilogram, rejuvenation, after 3 months, is sampled to the soil of American aloe root, measures the nutritional labeling in soilContent, result is as shown in table 2:
Table 2: the nutritional labeling comparison in American aloe root soil
As shown in Table 2, American aloe rejuvenation matrix of the present invention, can improve the nutrition of American aloe Soil SurroundingComposition.
Demonstration test 3:
Use rejuvenation culture medium I, contrast rejuvenation culture medium A, contrast rejuvenation culture medium B, contrast rejuvenation to cultivateBase C carries out rejuvenation to American aloe, and 100 every group, while using above-mentioned matrix, every American aloe root fertilising 3Kilogram, rejuvenation, after 3 months, is sampled to the soil of American aloe root, measures the bacterial number in soil,Result is as shown in table 3:
Table 3: the bacterial number comparison in American aloe root soil
As shown in Table 3, after the rejuvenation matrix rejuvenation American aloe of the present invention's formula, the fungal component of American aloe root systemHave significant increasing action, also, just because of the remarkable increase of fungal component, root soil nutritional labeling is remarkableImprove, the average attack rate of American aloe just can significantly reduce.
Above-mentioned example is just explanation technical conceive of the present invention and technical characterstic, can not limit this with thisThe protection domain of invention. Equivalent transformation or modification that all essence according to the present invention is done, all should be encompassed inWithin protection scope of the present invention.
Claims (7)
1. an American aloe rejuvenation culture medium, is characterized in that, comprises the raw material of following weight portion: 300～400Part agriculture organic material, the organoclay of 100～150 parts, 30～50 parts of calcareous, 0.5～1 part of fermentationsBacterium, 1～5 part of nutritive salt and 0.5～1 part of function bacterium;
Described zymophyte is EM microbial inoculum;
Described function bacterium comprises phosphate solubilizing bacteria, lactic acid bacteria, saccharomycete, photosynthetic bacteria, nitrogen-fixing bacteria, withered grass gemmaBacillus, its weight ratio is 2:1:1:1:1:2.
2. culture medium according to claim 1, is characterized in that, the organic material of described agricultural be stalk,Any one in bagasse, wood chip, beans bar, ight soil, or at least two kinds of mixtures that coordinate with arbitrary proportion.
3. culture medium according to claim 1, is characterized in that, described organoclay be fertile soil,Any one in peat soil, bentonite, diatomite, or at least two kinds of mixtures that coordinate with arbitrary proportion.
4. culture medium according to claim 1, is characterized in that, described calcareous be oyster shell whiting, donkeyThe mixture of bone meal, the weight ratio of oyster shell whiting, donkey bone meal is 2:1.
5. culture medium according to claim 1, is characterized in that, described nutritive salt is ammonium nitrate, 7Water magnesium sulfate, potassium dihydrogen phosphate, potassium nitrate, inositol, nicotinic acid, thiamine hydrochloride, puridoxine hydrochloride, sweetThe mixture that propylhomoserin mixes, each components by weight is: 2:1:1:1:1:1:1:1.5:0.5.
6. a preparation method for the American aloe rejuvenation culture medium described in claim 1～5 any one, comprisesFollowing steps:
(1), agriculture organic material is crushed to 1～2mm; Organoclay is crossed 100～150 mesh sieves, removes largeParticulate matter, retains the lower organoclay of sieve; Calcareous specification is, particle diameter 300～500nm; Proportionally claimGet zymophyte and the organic material of agricultural after treatment, organoclay and calcareous, by organic agricultural material, organoclay,Calcareously proportionally mix;
(2) the organic material of paving one deck agricultural, organoclay, calcareous mixture, spread one deck zymophyte uniformly on it;Repave the organic material of one deck agricultural, organoclay, calcareous mixture, on it, spread uniformly again one deck zymophyte;So stack, stacks into the buttress of high 1 meter, wide 1 meter, long 3 meters; In buttress, every 50 centimetres of intersections are putEnter ventilation duct one deck of 2 cun, place vertically and put a ventilation duct every 1.5 meters, on ventilation duct, have openning hole in advance,Ensure the turnover of sweat air free; Stacking will be loosened, and must not make real the not covering such as handy Polypropylence Sheet;
(3), measure temperature every day, within 3-4 days, compost each point temperature exceedes more than 60 DEG C, turn over throw buttress, then pressStep 2 continues to stack into the buttress of high 1 meter, wide 1 meter, long 3 meters; Continue about 3 days, then turn over and throw oneInferior, turn over after throwing and do not continue to heat up, can judge that fermentation completes, can ferment general 14-18 days autumn and winterGood, just can ferment about 6-9 days summer;
(4) product fermenting is mixed with nutritive salt, the function bacterium of described ratio and obtain American aloe againStrong culture medium.
7. a using method for the American aloe rejuvenation culture medium described in claim 1～5 any one, comprisesFollowing steps:
(1), in advance by organic agricultural material, organoclay, calcareous mixing, add zymophyte and ferment;
(2), after the fermentation material fermenting, be ready to nutritive salt, function bacterium the previous day that applies rejuvenation culture medium;
(2) applied fertilizer the same day, the fermentation material fermenting, nutritive salt, function bacterium were proportionally mixed;
(3) every American aloe root applies fertilizer 2～5 kilograms.
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| CN106359091A (en) * | 2016-08-30 | 2017-02-01 | 柳州市泓吉农业科技有限公司 | Culture medium for dendrobium officinale tissue culture seedling |
| CN107018905B (en) * | 2017-05-17 | 2018-11-09 | 天津润松生态科技发展有限公司 | A kind of bottle orchid test tube seedling preserving seed method |
| CN107182792A (en) * | 2017-07-19 | 2017-09-22 | 合肥申沃园艺有限公司 | A kind of Cunninghamia tissue culture method |
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| CN101993293A (en) * | 2009-08-31 | 2011-03-30 | 夏荣堂 | Rice reviving and tillering agent |
| CN102217469A (en) * | 2010-04-19 | 2011-10-19 | 陆振冈 | Organic cultivation medium and application method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993293A (en) * | 2009-08-31 | 2011-03-30 | 夏荣堂 | Rice reviving and tillering agent |
| CN102217469A (en) * | 2010-04-19 | 2011-10-19 | 陆振冈 | Organic cultivation medium and application method thereof |
| CN102653502A (en) * | 2012-04-09 | 2012-09-05 | 马鞍山科邦生态肥有限公司 | Low-cost water retention fertilizer and preparation method thereof |
Non-Patent Citations (1)
| Title |
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| 发酵粉碎麦秸和过筛炉渣混合制备的有机生态无土栽培基质研究;范广璞等;《北方园艺》;20131015(第19期);第22-25页 * |
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