CN104897839A - Multi-component comprehensively quantificational method for assessing and controlling quality of traditional Chinese medicine and application - Google Patents
Multi-component comprehensively quantificational method for assessing and controlling quality of traditional Chinese medicine and application Download PDFInfo
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- 240000001745 Rheum palmatum Species 0.000 claims abstract description 5
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- Medicines Containing Plant Substances (AREA)
Abstract
The application of the invention provides a multi-component comprehensively quantificational method for assessing and controlling the quality of traditional Chinese medicine and application. The method for assessing and controlling the quality of traditional Chinese medicine combines measurement of chemical content and measurement of biological potency, focuses on comprehensively quantificational assessment on the quality of the rheum palmatum herbs or medicinal slices through biological potency, and establishes the equivalent computation method for components causing the rheum palmatum purgative effect; the method can be applied to preliminary assessment on the quality of rheum palmatum herbs or medicinal slices with different producing areas, different batches and different growth years.
Description
Technical field
The application relates to a kind of evaluation method of traditional Chinese medicine quality of polycomponent comprehensive quantification.
Background technology
Medicine quality evaluated is traditional Chinese medicine research and the focus in production and difficult point always, is also important foundation and the key of the modernization of Chinese medicine.At present, the pattern of medicine quality evaluated most reference phytochemistry and Western medicine quality assessment, adopts the content of some chemical composition in single mensuration medicinal material to evaluate the quality of medicinal material.From traditional traditional Chinese medicine viewpoint, the control of single component is difficult to the effect really reflecting Chinese medicine, especially compound preparation, detects any one index components and all can not reflect its overall curative effect embodied.Biological assessment is because having the technical advantages such as drug effect is relevant, entirety is controlled, meet quality standard control model and the method [1] of traditional Chinese medicine feature, one of important development direction having become quality standards in Chinese drugs, and had pertinent literature to report [2,3].
Rheum officinale another name general, Huang Liang, fire ginseng, skin as etc., be one of China's " Chinese medicine four-dimensional ".3 important bases of the genuine rhubarb medicinal material that 2010 editions " Chinese Pharmacopoeia " records are former to be comprised: the dry root welding technology [4] of polygonum rheum palmatum Rheum palmatum L., Rheum tanguticum Maxim Rheum tanguticum Maxim.ex Balf. or Rheum officinale Rheum officinale Baill., main product is in extremely frigid zones such as the southeast of Gansu, western Sichuan, East of Qinghai Province, Eastern Tibet, there is heat and toxic materials clearing away, purging intense heat and detonicating, cooling blood and hemostasis, promoting blood circulation, rushes down clearly damp and hot effect [5].But existing rhubarb medicinal material quality control standard, most evaluation index [4] measured using the dissociated anthraquinone Aglycones content after hydrochloric acid hydrolysis as its quality good or not, the clinical efficacy degree of association of this and rheum officinale relieving constipation by purgation is strong, can not the overall quality comprehensively evaluating rheum officinale.Existing document shows [6,7,8], and in rheum officinale, Anthraquinones and dianthrone constituents are the main pharmacodynamics compositions of its relieving constipation by purgation.
The definition of biological value: the degree that so-called biological value normally completes certain specific physiological action with it is weighed as index, and standard reference thing compares as 100% and obtains, therefore biological value is relative value, can be greater than or less than 100%.
Document [9,10] is although relate to and rush down titration based on causing of mouse compound diphenoxylate Constipation Model, but what all adopt in document is the soak by water crude extract administration of rheum officinale, can not be concrete reflect singlely causes intensity under the rushing down of rushing down effect components, and this also just cannot be associated with its assay result.Therefore be a kind of rough titration.
In view of rhubarb decoction pieces is the clinical conventional Chinese medicine for constipation, chronic kidney disease, disease in the liver and gallbladder, how to evaluate different batches, Different sources, the rhubarb decoction pieces quality stability of separate sources, homogeneity be the difficult point that need pay close attention to, solve of pendulum in face of people, therefore develop a kind of estimation of biological potency method that can reflect that rheum officinale rushes down lower curative effect on the whole and seem very necessary and urgent.
List of references
[1] Xiao little He, Wang Jiabai, Yan Dan. the research and apply of biological assessment in quality standards in Chinese drugs [J]. World Science technology-TCM Modernization, 2014,16 (3): 514-518.
[2] Li Hanbing, Yan Dan, Wang Jia uncle etc. based on the biological assessment [J] of the Radix Isatidis quality that neuraminidase activity detects. Acta Pharmaceutica Sinica, 2009,44 (2): 162-166.
[3] Li Hanbing, Yan Dan, Xiao little He etc. the Radix Isatidis quality biomass evaluation method detected based on antiviral activity and optimizing research [J]. Chinese herbal medicine, 2011,42 (8): 1560-1565.
[4] the Pharmacopoeia of People's Republic of China council. the Pharmacopoeia of the People's Republic of China () [S]. Beijing: China Medical Science Press, 2010:22.
[5] Zhang Tingmo. Clinical Chinese Materia Medica [M]. Shanghai science tech publishing house, 2006,8:136-137.
[6] Mu Yikun, Li Malin, Yang Weimin. rheum officinale inquires into [J] to intestines and stomach Function and its mechanisms. Medical review, 2003,9 (2): 125-126.
[7] Zhang Dandan. clinical pharmacology research [J] of rheum officinale. Chinese traditional Chinese medicine modern distance education, 2011,9 (17): 68-69.
[8] Luo Pei, Xu Xiangzhen, Tan Zhenghuai. free anthraquinones extracted from rheum study on mechanism [J]. Pharmacology and Clinics of Chinese Materia Medica, 2013,29 (3): 88-90.
[9] Wang Jiabai, Li Huifang, Xiao little He etc. based on the research [J] of the rheum officinale minor biological cycling method of purgatives medicine effect model. China Association of Traditional Chinese Medicine's Chinese patent drug academic discuss collection of thesis, 2007,08,01:305-307.
[10] Li Huifang, Wang Jiabai, Xiao little He etc. cause and rush down the technique study [J] of bioactivity for rheum officinale quality evaluation. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2008,33,11:1309-1311.
Summary of the invention
First object of the present invention is to provide a kind of medicine quality evaluated method.
Medicine quality evaluated method of the present invention, comprises the content that (1) measures the active component relevant to the clinical efficacy of described Chinese medicine; (2) biological value of described active component is measured; (3) utilize following formula I to calculate the effect components equivalent value of the active component of described Chinese medicine: (4) utilize effect components equivalent value to obtain the actual amount numerical value of medicinal material by suitably converting;
EV=A
1× C
1+ A
2× C
2+ ... A
i× C
i-------formula I
Wherein EV represents the effect components equivalent value of active component;
A
1represent the effect components equivalent coefficient of active component 1;
C
1represent the content (mg/g) of active component 1 in every 1 gram of Chinese medicine;
A
2represent the effect components equivalent coefficient of active component 2;
C
2represent the content (mg/g) of the active component 2 in every 1 gram of Chinese medicine;
A
irepresent the effect components equivalent coefficient of active component i;
C
irepresent the content (mg/g) of active component 1 in every 1 gram of Chinese medicine.
Second object of the present invention is to provide a kind ofly rushes down based on rheum officinale crude drug or causing of rhubarb decoction pieces the method that effect equivalence factor regulates the consumption of rheum officinale crude drug or rhubarb decoction pieces.
Of the present inventionly rush down effect equivalence factor regulate the method for the consumption of rheum officinale crude drug or rhubarb decoction pieces to comprise the following steps based on causing of rheum officinale crude drug or rhubarb decoction pieces:
A () adopts the content that liquid phase chromatography measures aloe-emodin-8-o-β-D-Glucose glycosides in described rheum officinale crude drug or rhubarb decoction pieces, Rhein-8-o-β-D-Glucose glycosides, Chrysophanol-8-o-β-D-Glucose glycosides, archen-8-o-β-D-Glucose glycosides, Physcion-8-o-β-D-Glucose glycosides, aloe-emodin, Rhein, archen, Chrysophanol, Physcion, Sennoside A, Sennoside B amount to 12 kinds of active components;
B () utilizes formula II to calculate causing of rhubarb medicinal material to be measured to rush down effect components equivalent value EV:
EV=1.000 × C
1+ 0.647 × C
2+ 0.659 × C
3+ 0.722 × C
4+ 0.455 × C
5+ 0.475 × C
6+ 0.559 × C
7+ 0.383 × C
8+ 0.468 × C
9+ 0.273 × C
10+ 0.263 × C
11+ 0.521 × C
12-------formula II
C
1represent the content of the Sennoside A in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
2represent the content of the Sennoside B in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
3represent the content of the aloe-emodin-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
4represent the content of the Rhein-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
5represent the content of the Chrysophanol-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
6represent the content of the archen-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
7represent the content of the Physcion-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
8represent the content of aloe-emodin in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
9represent the content of the Rhein in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
10represent the content of the Chrysophanol in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
11represent the content of the archen in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
12represent the content of the Physcion in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g.
C () rushes down according to causing of calculating the consumption that effect components equivalent value EV regulates rheum officinale crude drug or rhubarb decoction pieces.
In one embodiment, step (a) comprising:
Chromatographic column octadecylsilane chemically bonded silica is filling agent, C18 post;
Mobile phase: with methyl alcohol (A)-0.1% phosphate aqueous solution (B) for mobile phase;
Flow rate of mobile phase: 0.1-0.4ml/min;
Elution requirement: 0 ~ 5min:38 → 43 (A), 5 ~ 7min:43 → 52 (A), 7 ~ 12min:52 → 57 (A), 12 ~ 15min:57 → 72 (A), 15 ~ 17min:72 → 79 (A), 17 ~ 20min:79 → 85 (A);
Determined wavelength: 250-420nm;
Column temperature: 22-42 DEG C;
Sample size: 1.0-4.0 μ l;
Theoretical cam curve calculates should be not less than 50000 by Physcion;
The preparation of reference substance solution:
Solvent is made with hplc grade methanol; Precision takes 10 reference substances such as aloe-emodin-8-o-β-D-Glucose glycosides and is placed in brown volumetric flask in right amount, is mixed with the mixing reference substance solution that concentration is 50 μ g/ml, 55 μ g/ml, 35 μ g/ml, 20 μ g/ml, 15 μ g/ml, 8 μ g/ml, 10 μ g/ml, 7 μ g/ml, 8 μ g/ml, 7 μ g/ml.
The preparation of need testing solution to be measured:
Get Rhubarb 0.20g (crossing No. four sieves), accurately weighed, put in the brown volumetric flask of 50ml, precision adds 25ml methyl alcohol, weighed weight, ultrasonic process 60 minutes, weighed weight again, supplies the weight of less loss, shakes up with methyl alcohol, get supernatant and cross 0.22 μm of miillpore filter, get subsequent filtrate as need testing solution.
Measure: accurate absorption reference substance solution and each 2 μ l sample introductions of need testing solution measure respectively, calculate peak area, measure content according to one point external standard method.
Ultra Performance Liquid Chromatography instrument: preferred Waters Acquity Ultra Performance Liquid Chromatography instrument (water generation company of the U.S.) in the present invention, is furnished with Waters PDA Detector (diode array detector), is furnished with automatic sampler, Empower 2 chromatographic work station.
Chromatographic column: in the present invention, preferred chromatographic column is: Acquity UPLC BEH C18 post (1.7 μm, 2.1 × 100mm).
Mobile phase: with methyl alcohol (A)-0.1% phosphate aqueous solution (B) for mobile phase, the mobile phase that the present invention adopts is selected, be through inventor preferably to go out in great many of experiments heuristic process, as inventor adopts methyl alcohol (A)-0.1% phosphate aqueous solution (B) respectively at (45:55), (55:45), (65:35) and under the ratio of gradient elution compare, at the determined wavelength of 410nm, the column temperature of 30 DEG C, the flow velocity of 0.2ml/min, under the condition of the sample size of 2 μ l, Waters Acquity Ultra Performance Liquid Chromatography instrument is utilized to detect, find under condition of gradient elution, to greatest extent interfering material 10 target peaks can be separated, obtain degree of separation and all satisfactory chromatographic peak of chromatographic peak profile.(see Fig. 1)
Flow rate of mobile phase: 0.2ml/min; Inventor is detecting respectively under 0.1ml/min, 0.2ml/min and 0.3ml/min, 0.4ml/min flow velocity, finds all can reach requirement of experiment, and preferred 0.2ml/min is as detection flow velocity.
Column temperature: 30 DEG C; Inventor detects under 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 38 DEG C, 42 DEG C column temperatures, finds all can reach requirement of experiment, and preferably 30 DEG C of conducts detect column temperature.
Determined wavelength: 340nm; Sennoside A reference substance solution, Sennoside B reference substance solution are adopting diode array detector (PDA detecting device) in the interscan of 200-450nm wavelength coverage by inventor, find to have at 254nm and 410nm wavelength place to absorb more by force, preferred 410nm is as determined wavelength.
Sample size: 2 μ l; Inventor detects under 1 μ l, 2 μ l, 2.5 μ l, 3 μ l, 3.5 μ l, 4 μ l column temperatures, finds all can reach requirement of experiment, and preferably 2 μ l are as detection sample size.
The preparation of reference substance solution: precision takes 10 reference substances such as aloe-emodin-8-o-β-D-Glucose glycosides and is placed in brown volumetric flask in right amount, is mixed with the mixing reference substance solution that concentration is 50 μ g/ml, 55 μ g/ml, 35 μ g/ml, 20 μ g/ml, 15 μ g/ml, 8 μ g/ml, 10 μ g/ml, 7 μ g/ml, 8 μ g/ml, 7 μ g/ml.
The preparation of need testing solution: using 70% methyl alcohol, 80% methyl alcohol, 90% methyl alcohol, absolute methanol as Extraction solvent, extraction time is respectively 40 minutes, 50 minutes, 60 minutes, 80 minutes, 90 minutes, adopts ultrasound wave extraction, heating and refluxing extraction method as extracting method.Be preferably Extraction solvent with absolute methanol, ultrasound wave extracts 60 minutes as need testing solution preparation method of the present invention.
B. the content of Sennoside A and Sennoside B in test sample (rhubarb medicinal material namely to be detected or medicine materical crude slice sample) is measured:
Chromatographic column: be filling agent with octadecylsilane chemically bonded silica, C18 post;
Mobile phase: with acetonitrile (A)-0.1% phosphate aqueous solution (B) for mobile phase;
Flow rate of mobile phase: 0.2ml/min;
Elution requirement: 0 ~ 5min:8 → 12 (A), 5 ~ 10min:12 → 13 (A), 10 ~ 15min:13 → 15 (A), 15 ~ 20min:15 → 17 (A), 20 ~ 25min:17 → 21 (A), 25 ~ 30min:21 → 60 (A);
Determined wavelength: 340nm;
Column temperature: 30 DEG C;
Sample size: 2.0 μ l;
Theoretical cam curve calculates should be not less than 10000 by Sennoside A;
The preparation of the reference substance solution of Sennoside A, Sennoside B:
With containing 0.1%NaHCO
345% methanol aqueous solution make solvent; Precision takes Sennoside A, Sennoside B reference substance is placed in brown volumetric flask in right amount, and being mixed with concentration is 70 μ g/ml, 40 μ g/ml mixing reference substance solution.
The preparation of need testing solution to be measured:
Get Rhubarb 0.20g (crossing No. four sieves), accurately weighed, put in the brown volumetric flask of 50ml, precision adds containing 0.1%NaHCO
345% methanol aqueous solution 25ml, weighed weight, ultrasonic process 40 minutes, more weighed weight, use 0.1%NaHCO
345% methanol aqueous solution supply the weight of less loss, centrifugal 10 minutes, get supernatant and cross 0.22 μm of miillpore filter, get subsequent filtrate as need testing solution.
Measure: accurate absorption reference substance solution and each 2 μ l sample introductions of need testing solution measure, and calculate peak area, measure content according to one point external standard method.
C. causing to rush down and tiring of 12 chemical compositions such as aloe-emodin-8-o-β-D-Glucose glycosides, Sennoside A is measured:
With ICR male mice 140, body weight 20g, is divided into 14 groups, often organizes 10, test front 12 h fast and can't help water, and wherein first group as normal group, not to and any modeling and administration; As model control group, only give and cause Constipation Model with compound ground phenol promise ester sheet for second group; All the other 12 groups corresponding 12 effect components administrations respectively.First use compound ground phenol promise ester sheet (dosage 50mgkg
-1, 60mgkg
-1) gavage carries out modeling, gastric infusion after modeling 0.5h, 1h, 2h, (wherein the concentration of 12 chemical composition monomers such as aloe-emodin-8-o-β-D-Glucose glycosides, Sennoside A is 1mg/ml, 0.8mg/ml, 0.6mg/ml, 0.4mg/ml to dosage 0.4-0.8ml, 37 DEG C of hot water dissolvings), after administration, water is can't help in fasting, collect stool quality after 6h, 8h, 10h, as inspection target, cause as rheum officinale monomer component and rush down titration (U/mg).
Through investigating, finally preferred first to use compound ground phenol promise ester sheet (dosage 60mgkg
-1) gavage carries out modeling, gastric infusion after modeling 1h, (wherein the concentration of 12 chemical composition monomers such as aloe-emodin-8-o-β-D-Glucose glycosides, Sennoside A is 1mg/ml to dosage 0.4ml, 37 DEG C of hot water dissolvings), collect stool quality after 8h after administration, rush down titration method as of the present invention causing.
D. rheum officinale causes the structure rushing down effect components equivalent formula:
What obtain according to each chemical monomer composition of step c causes to rush down and tires, tire as with reference to (rushing down lower coefficient for 1) using Sennoside A, the causing to rush down of all the other 11 effect components is tired in contrast, obtains 11 and causes and rush down causing of effect components and rush down effect components equivalent coefficient.Causing of 12 compositions such as aloe-emodin-8-o-β-D-Glucose glycosides, Sennoside A is rushed down effect components equivalent coefficient and be multiplied by content (mg/g) respectively, then by every addition, obtain rheum officinale and cause and rush down effect components equivalent formula II.
EV=1.000 × C
1+ 0.647 × C
2+ 0.659 × C
3+ 0.722 × C
4+ 0.455 × C
5+ 0.475 × C
6+ 0.559 × C
7+ 0.383 × C
8+ 0.468 × C
9+ 0.273 × C
10+ 0.263 × C
11+ 0.521 × C
12-------formula II
In formula:
EV represents effect components equivalent value
C
1represent the content (mg/g) of Sennoside A in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
2represent the content (mg/g) of Sennoside B in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
3represent the content (mg/g) of aloe-emodin-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
4represent the content (mg/g) of Rhein-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
5represent the content (mg/g) of Chrysophanol-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
6represent the content (mg/g) of archen-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
7represent the content (mg/g) of Physcion-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
8represent the content (mg/g) of aloe-emodin in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
9represent the content (mg/g) of Rhein in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
10represent the content (mg/g) of Chrysophanol in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
11represent the content (mg/g) of archen in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
C
12represent the content (mg/g) of Physcion in every 1 gram of rhubarb medicinal material/medicine materical crude slice;
Coefficient before each subitem formula rushes down effect components equivalent coefficient for causing, meaning representated by it is: to tire as object of reference under the rushing down of Sennoside A, to tire under the rushing down of other 11 monomer components reference gained with it, namely 1mg/ml cause the ratio of tiring produced with the Sennoside A of 1mg/ml of tiring rushing down composition and produce.
Effect components equivalent value: refer to a certain large class in Chinese crude drug and there is the biological value of the effect components (active component) of similar pharmacological action compared with reference substance, obtain tire (namely equivalent is tired) that each composition is equivalent to how many reference substance.The content of each effect components in medicinal material is multiplied by equivalent to tire and obtain the equivalent value of this effect components, finally the equivalent value of each effect components is added and, namely obtain the equivalent value that this medicinal material is equivalent to how many reference substance, i.e. effect components equivalent value.Such as in the present invention, causing having in rhubarb medicinal material the equivalent that the several main pharmacodynamics composition conversions rushing down effect become reference substance Sennoside A, being the effect components equivalent value of Sennoside A.
Compared with prior art, the traditional Chinese medicine quality of the application's polycomponent comprehensive quantification comments control method to be more suitable for the therapeutic evaluation of rhubarb medicinal material, more comprehensively can embody the intensity that rheum officinale rushes down lower curative effect, meets the developing direction that traditional Chinese medicine quality comments control.Particularly, compared with total free anthraquinone detection method of content in existing rhubarb medicinal material or medicine materical crude slice, the quality of the application comments control method tool to have the following advantages:
1. changing the quality evaluating rhubarb medicinal material with single survey total free anthraquinone content, adding the assay (associativity anthraquinone glucosides, sennoside constituents) of the index effect components relevant to rushing down lower curative effect.
2. add the minor biological cycling based on compound diphenoxylate Constipation Model, embody intensity under the rushing down between different activities composition more accurately, be more conducive to the assessment of rheum officinale clinical efficacy.
3, obtain rheum officinale first and cause the equivalent coefficient rushing down effect components, provide reference for rheum officinale difference causes the pharmacological research rushing down effect components.Obtain rheum officinale effect components equivalent value first, intensity under the rushing down of different batches rhubarb medicinal material (or medicine materical crude slice) can be evaluated by the size of effect components equivalent value, reference can be provided for clinical rational consumption.
4, the evaluation method based on effect components equivalent value is that traditional Chinese medicine quality controls to provide new thinking and schema reference.Such as when evaluating heat-clearing and detoxifying herb honeysuckle, can take chlorogenic acid as reference substance, the composition conversions such as isochlorogenic acid, myristic acid, forulic acid, caffeic acid, protocatechuic acid are become the effect components equivalent value of chlorogenic acid, reflect with this effect power that honeysuckle is clearing heat and detoxicating, antibacterial.Such as evaluate toxic traditional Chinese medicine monkshood time, wherein diester-type alkaloids has stronger toxicity, therefore can take aconitine as reference substance, Hypaconitine, mesaconitine, mesaconine etc. are converted to the effect components equivalent value of aconitine, the toxicity evaluating monkshood with the size of effect components equivalent value is strong and weak.
Inventor is according on the basis of the chemical composition of rheum officinale, pharmacological action and biliographic data, through repeatedly groping, finally establish and a set ofly cause the detection method of rushing down titration in conjunction with effect components assay and effect components, establish the computing formula causing and rush down effect components equivalent on this basis, cause rush down effect components equivalent value can the quality of concentrated expression rhubarb medicinal material, and provide reference for the biological assessment method of rheum officinale.
Accompanying drawing explanation
Fig. 1 shows aloe-emodin-8-o-β in rheum officinale reference substance solution-D-Glucose glycosides isochrome spectrogram (410nm wavelength);
Fig. 2 shows aloe-emodin-8-o-β in rheum officinale need testing solution-D-Glucose glycosides isochrome spectrogram (410nm wavelength);
Embodiment
Describe the embodiment of the application below by embodiment, one of ordinary skill in the art appreciates that these specific embodiments only show object in order to reach the application and the enforcement technical scheme selected, is not the restriction to technical scheme.According to the instruction of the application, be obvious in conjunction with the improvement of prior art to technical scheme, all belong to the scope of the application's protection.
The material adopted in following examples, except where noted, all the other are commercially available.
Embodiment 1 (10 kinds of content determinations of active components such as aloe-emodin-8-o-β-D-Glucose glycosides in rhubarb medicinal material)
Test sample: rheum officinale test sample (rhubarb medicinal material sample namely to be detected)
Reference substance: aloe-emodin-8-o-β-D-Glucose glycosides (lot number 141225, for assay in order to 98.78), Rhein-8-o-β-D-Glucose glycosides (lot number 141211, for assay in order to 98.57%), Chrysophanol-8-o-β-D-Glucose glycosides (lot number 141220, for assay in order to 99.42%), archen-8-o-β-D-Glucose glycosides (lot number 141209, for assay in order to 98.67%), Physcion-8-o-β-D-Glucose glycosides (lot number 141012, for assay in order to 99.14%), by Chengdu, clo agate biotechnology company provides,
Aloe-emodin (lot number 110795-201007, for assay in order to 98.05%), Rhein (lot number 110757-200206, for assay in order to 98.65%), archen (lot number 110756, for assay in order to 99.55%), Chrysophanol (lot number 110796-201118, for assay in order to 99.50%), Physcion (lot number 110758-201013, for assay in order to 99.80%) is provided by National Institute for Food and Drugs Control.
1. experimental apparatus: Waters Acquity Ultra Performance Liquid Chromatography instrument (water generation company of the U.S.), is furnished with Waters PDA Detector (diode array detector), automatic sampler, Empower 2 chromatographic work station, (1.7 μm, Acquity UPLC BEH C18 post, 2.1 × 100mm), XS-205 electronic balance (METTLER TOLEDO), AL-204 electronic balance (METTLER TOLEDO), Ultrasound Instrument (Xin Chen bio tech ltd, Nanjing, 40KHz).
2. chromatographic condition:
Mobile phase: with methyl alcohol (A)-0.1% phosphate aqueous solution (B) for mobile phase;
Flow rate of mobile phase: 0.2ml/min;
Elution requirement: 0 ~ 5min:38 → 43 (A), 5 ~ 7min:43 → 52 (A), 7 ~ 12min:52 → 57 (A), 12 ~ 15min:57 → 72 (A), 15 ~ 17min:72 → 79 (A), 17 ~ 20min:79 → 85 (A);
Determined wavelength: 410nm;
Column temperature: 30 DEG C;
Sample size: 2.0 μ l;
Theoretical cam curve calculates should be not less than 100000 by Physcion;
3. the preparation of reference substance solution:
Solvent is made with hplc grade methanol; Precision takes 10 reference substances such as aloe-emodin-8-o-β-D-Glucose glycosides and is placed in brown volumetric flask in right amount, is mixed with the mixing reference substance solution that concentration is 50 μ g/ml, 55 μ g/ml, 35 μ g/ml, 20 μ g/ml, 15 μ g/ml, 8 μ g/ml, 10 μ g/ml, 7 μ g/ml, 8 μ g/ml, 7 μ g/ml.
4. the preparation of need testing solution to be measured: get Rhubarb 0.20g (crossing No. four sieves), accurately weighed, put in the brown volumetric flask of 50ml, precision adds containing 25ml methyl alcohol, weighed weight, ultrasonic process 60 minutes, weighed weight again, supplies the weight of less loss, shakes up with methyl alcohol, get supernatant and cross 0.22 μm of miillpore filter, get subsequent filtrate as need testing solution.
5. test sample measures: carry out assay to the rheum officinale test sample of 10 batches of Different sources as stated above.Measurement result is in table 1.
The rhubarb medicinal material combined anthraquinone of table 1 10 batches of Different sources and dissociated anthraquinone assay result (n=3)
Embodiment 2 assay of Sennoside A, Sennoside B (in the rhubarb medicinal material)
Test sample: rheum officinale test sample (rhubarb medicinal material sample namely to be detected)
Reference substance: Sennoside A (lot number 13122701, for assay in order to 98.58%), Sennoside B (lot number 13062606, for assay in order to 98.35%), by Chengdu, Pu Rui biotechnology company provides;
Waters Acquity Ultra Performance Liquid Chromatography instrument (water generation company of the U.S.), is furnished with Waters PDA Detector (diode array detector), automatic sampler, Empower 2 chromatographic work station, (1.7 μm, Acquity UPLC BEH C18 post, 2.1 × 100mm), XS-205 electronic balance (METTLER TOLEDO), AL-204 electronic balance (METTLER TOLEDO), Ultrasound Instrument (Xin Chen bio tech ltd, Nanjing, 40KHz).
The preparation of the reference substance solution of Sennoside A, Sennoside B:
With containing 0.1%NaHCO
345% methanol aqueous solution make solvent; Precision takes Sennoside A, Sennoside B reference substance is placed in brown volumetric flask in right amount, is mixed with the mixing reference substance solution that concentration is 70 μ g/ml, 40 μ g/ml.
Folium sennae need testing solution preparation method:
Get Senna Leaf (crossing No. four sieves) about 0.20g, accurately weighed, put in the brown volumetric flask of 50ml, precision adds 0.1%NaHCO
3aqueous solution 25ml, weighed weight, ultrasonic 30 minutes (controlling water temperature less than 30 DEG C), more weighed weight, use 0.1%NaHCO
3aqueous solution supplies the weight of less loss, centrifugal 5 minutes, gets supernatant and crosses 0.22 μm of miillpore filter, get subsequent filtrate as need testing solution.
Determination method: accurate absorption reference substance solution and each 2 μ l of need testing solution respectively, injects Ultra Performance Liquid Chromatography instrument, measures chromatographic peak area, calculate and get final product by external standard method.
The rhubarb medicinal material Sennoside A of table 2 10 batches of Different sources, Sennoside B assay result (n=3)
Embodiment 3 (rheum officinale causes the detailed examination of the calculating of rushing down mensuration that effect components tires and equivalent coefficient)
ICR male mice 140, body weight 20g, is divided into 14 groups, often organizes 10, test front 12 h fast and can't help water, and wherein first group as normal group, not to and any modeling and administration; As model control group, only give and cause Constipation Model with compound ground phenol promise ester sheet for second group; All the other 12 groups corresponding 12 effect components administrations respectively.First use compound ground phenol promise ester sheet (dosage 60mgkg
-1) gavage carries out modeling, gastric infusion after modeling 1h, (wherein the concentration of 12 kinds of active components such as aloe-emodin-8-o-β-D-Glucose glycosides, Sennoside A is 1mg/ml to dosage 0.4ml, 37 DEG C of hot water dissolvings), after administration, water is can't help in fasting, collect stool quality after 8h, each active component being rheum officinale causes and rushes down the U/mg that tires, and causing using 1mg stool quality as 1U is rushed down and tired.The causing of 12 kinds of active components such as aloe-emodin-8-o-β-D-Glucose glycosides, Sennoside A rush down titration result, as follows in table 3.:
The titration result (n=3) of each composition of table 3
Causing to rush down and tire as reference using Sennoside A, the causing to rush down of all the other 11 kinds of active components is tired by comparison, obtains this active component and rushes down effect components equivalent coefficient in causing of Sennoside A.The results are shown in Table 4.
Effect components equivalent coefficient result of calculation (n=3) is rushed down in causing of table 4 12 kinds of active components
Special instruction: causing the calculating of rushing down effect components equivalent coefficient is that causing of self containing in (rheum officinale) medicinal material rushes down the strongest material of effect as reference, what finally rush down lower composition with other mensuration tires by comparison, and this ratio is the equivalent coefficient of this active component.Due to animal used as test individuality between the factor such as difference, operate miss, the causing of often kind of composition is rushed down effect components equivalent coefficient and be there is certain fluctuation range, and present inventor thinks that scope in table 4 is in tolerance interval.
Embodiment 4 calculates causing of the rhubarb medicinal material of Different sources 10 batches and rushes down effect components equivalent value
According to the data that above embodiment 1,2,3 obtains, calculate according to formula 1, obtain causing of this rhubarb medicinal material/medicine materical crude slice of 10 batches and rush down effect components equivalent value.The results are shown in Table 5.
Effect components equivalent value result of calculation (n=3) in table 5 10 batches of Different sources rhubarb medicinal materials
The embody rule of embodiment 5 effect components equivalent
Can find out according to above 1-4 example result of implementing, Different sources, different base are former, the rhubarb medicinal material of different growth years, according to the quality control standard of 2010 editions " Chinese Pharmacopoeias ", it is all standard compliant medicinal material, but it causes and rushes down effect components equivalent and there is larger difference, therefore its rush down under pharmacologically active intensity just there is larger difference.Such as, under the rushing down of Rheum tanguticum Maxim, intensity is far longer than intensity under the rushing down of sorrel, therefore should note distinguishing consumption in clinical practice.Under same dose requirements, cause rush down the higher rhubarb medicinal material of effect components equivalent value by convert should be suitable reduction consumption; Otherwise, cause rush down the lower rhubarb medicinal material of effect components equivalent value can according to conversion suitably increase consumption, ensure validity and the security of rheum officinale clinical efficacy with this.Such as cause for the difference of 10 batches of rhubarb medicinal materials in table 5 and rush down effect components equivalent and by being converted into the actual use amount of the conforming medicinal material of equivalent be:
Table 6 difference causes the actual use amount of the rhubarb medicinal material rushing down effect components equivalent
Special instruction: for the data in table 6, calculate in the following manner: medicinal material is numbered the rhubarb medicinal material of 2, the actual measurement effect components equivalent value calculating its 1g medicinal material through method of the present invention is 41.611, if reach 40 equivalent values in Sennoside A, so medicinal material is numbered the clinical actual amount of rhubarb medicinal material of 2 should be 40/41.611=0.961g.
Medicinal material is numbered the rhubarb medicinal material of 3, the actual measurement effect components equivalent value calculating its 1g medicinal material through method of the present invention is 15.775, if reach 40 equivalent values in Sennoside A, so medicinal material is numbered the clinical actual amount of rhubarb medicinal material of 2 should be 40/15.775=2.536g.
In like manner can calculate the conforming actual amount of equivalent of the rheum officinale of different batches, namely can ensure the stability of quantity.
The foregoing is only the preferred embodiment of the application, not any formal and substantial restriction is made to the application.Those skilled in the art, not departing from the scope of technical scheme, a little change made, modifies the Equivalent embodiments being the application with the equivalent variations of differentiation when utilizing disclosed above technology contents; Meanwhile, all substantial technological according to the application to the change of any equivalent variations that above embodiment is done, modify with differentiation etc. all in the scope defined by claim of the application.
Claims (8)
1. a medicine quality evaluated method, comprises the content that (1) measures the active component relevant to the clinical efficacy of described Chinese medicine; (2) biological value of described active component is measured; (3) utilize following formula I to calculate the effect components equivalent value of the active component of described Chinese medicine: (4) utilize effect components equivalent value to obtain the actual amount numerical value of medicinal material by suitably converting;
EV=A
1× C
1+ A
2× C
2+ ... A
i× C
i-------formula I
Wherein EV represents the effect components equivalent value of active component;
A
1represent the effect components equivalent coefficient of active component 1;
C
1represent the content (mg/g) of active component 1 in every 1 gram of Chinese medicine;
A
2represent the effect components equivalent coefficient of active component 2;
C
2represent the content (mg/g) of the active component 2 in every 1 gram of Chinese medicine;
A
irepresent the effect components equivalent coefficient of active component i;
C
irepresent the content (mg/g) of active component 1 in every 1 gram of Chinese medicine.
2. the method for claim 1, wherein said Chinese medicine is rheum officinale crude drug or rhubarb decoction pieces, and described biological value causes to rush down biological value.
3. rush down based on rheum officinale crude drug or causing of rhubarb decoction pieces the method that effect equivalence factor regulates the consumption of rheum officinale crude drug or rhubarb decoction pieces, said method comprising the steps of:
A () adopts the content that liquid phase chromatography measures aloe-emodin-8-o-β-D-Glucose glycosides in described rheum officinale crude drug or rhubarb decoction pieces, Rhein-8-o-β-D-Glucose glycosides, Chrysophanol-8-o-β-D-Glucose glycosides, archen-8-o-β-D-Glucose glycosides, Physcion-8-o-β-D-Glucose glycosides, aloe-emodin, Rhein, archen, Chrysophanol, Physcion, Sennoside A, Sennoside B amount to 12 kinds of active components;
B () utilizes formula II to calculate causing of rhubarb medicinal material to be measured to rush down effect components equivalent value EV:
EV=1.000 × C
1+ 0.647 × C
2+ 0.659 × C
3+ 0.722 × C
4+ 0.455 × C
5+ 0.475 × C
6+ 0.559 × C
7+ 0.383 × C
8+ 0.468 × C
9+ 0.273 × C
10+ 0.263 × C
11+ 0.521 × C
12-------formula II
C
1represent the content of the Sennoside A in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
2represent the content of the Sennoside B in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
3represent the content of the aloe-emodin-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
4represent the content of the Rhein-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
5represent the content of the Chrysophanol-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
6represent the content of the archen-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
7represent the content of the Physcion-8-o-β-D-Glucose glycosides in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
8represent the content of aloe-emodin in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
9represent the content of the Rhein in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
10represent the content of the Chrysophanol in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
11represent the content of the archen in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g;
C
12represent the content of the Physcion in every 1 gram of rhubarb medicinal material/medicine materical crude slice, mg/g.
C () rushes down according to causing of calculating the consumption that effect components equivalent value EV regulates rheum officinale crude drug or rhubarb decoction pieces.
4. method as claimed in claim 3, wherein said step (a) comprising:
Chromatographic column octadecylsilane chemically bonded silica is filling agent, C18 post;
Mobile phase: with methyl alcohol (A)-0.1% phosphate aqueous solution (B) for mobile phase;
Flow rate of mobile phase: 0.1-0.4ml/min;
Elution requirement: 0 ~ 5min:38 → 43 (A), 5 ~ 7min:43 → 52 (A), 7 ~ 12min:52 → 57 (A), 12 ~ 15min:57 → 72 (A), 15 ~ 17min:72 → 79 (A), 17 ~ 20min:79 → 85 (A);
Determined wavelength: 250-420nm;
Column temperature: 22-42 DEG C;
Sample size: 1.0-4.0 μ l;
Theoretical cam curve calculates should be not less than 50000 by Physcion;
The preparation of reference substance solution:
Solvent is made with hplc grade methanol; Precision takes aloe-emodin-8-o-β-D-Glucose glycosides, Rhein-8-o-β-D-Glucose glycosides, Chrysophanol-8-o-β-D-Glucose glycosides, archen-8-o-β-D-Glucose glycosides, Physcion-8-o-β-D-Glucose glycosides, aloe-emodin, Rhein, archen, Chrysophanol, Physcion 10 reference substances are placed in brown volumetric flask in right amount, is mixed with the mixing reference substance solution that concentration is 50 μ g/ml, 55 μ g/ml, 35 μ g/ml, 20 μ g/ml, 15 μ g/ml, 8 μ g/ml, 10 μ g/ml, 7 μ g/ml, 8 μ g/ml, 7 μ g/ml;
The preparation of need testing solution to be measured:
Get Rhubarb 0.20g, cross No. four sieves, accurately weighed, put in the brown volumetric flask of 50ml, precision adds 25ml methyl alcohol, weighed weight, ultrasonic process 60 minutes, more weighed weight, the weight of less loss is supplied with methyl alcohol, shake up, get supernatant and cross 0.22 μm of miillpore filter, get subsequent filtrate as need testing solution;
Measure: accurate absorption reference substance solution and each 2 μ l sample introductions of need testing solution measure respectively, calculate peak area, according to external standard method content.
5. method as claimed in claim 3, wherein said step (a) comprising:
Measure test sample, the content of Sennoside A and Sennoside B in rhubarb medicinal material namely to be detected or medicine materical crude slice sample:
Chromatographic column: be filling agent with octadecylsilane chemically bonded silica, C18 post;
Mobile phase: with acetonitrile (A)-0.1% phosphate aqueous solution (B) for mobile phase;
Flow rate of mobile phase: 0.2ml/min;
Elution requirement: 0 ~ 5min:8 → 12 (A), 5 ~ 10min:12 → 13 (A), 10 ~ 15min:13 → 15 (A), 15 ~ 20min:15 → 17 (A), 20 ~ 25min:17 → 21 (A), 25 ~ 30min:21 → 60 (A);
Determined wavelength: 340nm;
Column temperature: 30 DEG C;
Sample size: 2.0 μ l;
Theoretical cam curve calculates should be not less than 10000 by Sennoside A;
The preparation of the reference substance solution of Sennoside A, Sennoside B:
With containing 0.1%NaHCO
345% methanol aqueous solution make solvent; Precision takes Sennoside A, Sennoside B reference substance is placed in brown volumetric flask in right amount, and being mixed with concentration is 70 μ g/ml, 40 μ g/ml mixing reference substance solution;
The preparation of need testing solution to be measured:
Get Rhubarb 0.20g, cross No. four sieves, accurately weighed, put in the brown volumetric flask of 50ml, precision adds containing 0.1%NaHCO
345% methanol aqueous solution 25ml, weighed weight, ultrasonic process 60 minutes, controls water temperature less than 35 DEG C, more weighed weight, uses 0.1%NaHCO
345% methanol aqueous solution supply the weight of less loss, centrifugal 10 minutes, get supernatant and cross 0.22 μm of miillpore filter, get subsequent filtrate as need testing solution;
Measure: accurate absorption reference substance solution and each 2 μ l sample introductions of need testing solution measure respectively, measure peak area, measure content according to one point external standard method.
6. method according to claim 5, the chromatographic condition that wherein said a step is determined is:
Mobile phase: with methyl alcohol (A)-0.1% phosphate aqueous solution (B) for mobile phase;
Flow rate of mobile phase: 0.2ml/min;
Elution requirement: 0 ~ 5min:38 → 43 (A), 5 ~ 7min:43 → 52 (A), 7 ~ 12min:52 → 57 (A), 12 ~ 15min:57 → 72 (A), 15 ~ 17min:72 → 79 (A), 17 ~ 20min:79 → 85 (A);
Determined wavelength: 410nm;
Column temperature: 30 DEG C;
Sample size: 2.0 μ l.
7. method as claimed in claim 3, wherein said step (b) comprising:
The effect components equivalent coefficient of active component Sennoside A is 1.000; The effect components equivalent coefficient of active component Sennoside B is 0.647; The effect components equivalent coefficient of active component aloe-emodin-8-o-β-D-Glucose glycosides is 0.659; The effect components equivalent coefficient of active component Rhein-8-o-β-D-Glucose glycosides is 0.722; The effect components equivalent coefficient of active component Chrysophanol-8-o-β-D-Glucose glycosides is 0.455; The effect components equivalent coefficient of active component archen-8-o-β-D-Glucose glycosides is 0.475; The effect components equivalent coefficient of active component Physcion-8-o-β-D-Glucose glycosides is 0.559; The effect components equivalent coefficient of active component aloe-emodin is 0.383; The effect components equivalent coefficient of active component Rhein is 0.468; The effect components equivalent coefficient of active component archen is 0.273; The effect components equivalent coefficient of active component Chrysophanol is 0.263; The effect components equivalent coefficient of active component aloe-emodin methyl ether is 0.521.
8. method according to claim 3, is characterized in that: described rheum officinale crude drug or rhubarb decoction pieces comprise the dry root of polygonum rheum palmatum Rheum palmatum L., Rheum tanguticum Maxim Rheum tanguticum Maxim.ex Balf. or Rheum officinale Rheum officinale Baill., rhizome and rhubarb decoction pieces.
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| CN114636779B (en) * | 2022-03-29 | 2024-05-24 | 陕西盘龙药业集团股份有限公司 | Construction method of three-conversion soup reference sample freeze-dried powder fingerprint and fingerprint thereof |
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