CN104788177A - Special water-retaining biological organic fertilizer for blueberries and preparation method thereof - Google Patents

Special water-retaining biological organic fertilizer for blueberries and preparation method thereof Download PDF

Info

Publication number
CN104788177A
CN104788177A CN201510149708.8A CN201510149708A CN104788177A CN 104788177 A CN104788177 A CN 104788177A CN 201510149708 A CN201510149708 A CN 201510149708A CN 104788177 A CN104788177 A CN 104788177A
Authority
CN
China
Prior art keywords
blueberry
microbial composite
liquid
fertilizer
special
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201510149708.8A
Other languages
Chinese (zh)
Other versions
CN104788177B (en
Inventor
马海林
孙蕾
刘方春
马丙尧
杜振宇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Academy of Forestry
Original Assignee
Shandong Academy of Forestry
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong Academy of Forestry filed Critical Shandong Academy of Forestry
Priority to CN201510149708.8A priority Critical patent/CN104788177B/en
Publication of CN104788177A publication Critical patent/CN104788177A/en
Application granted granted Critical
Publication of CN104788177B publication Critical patent/CN104788177B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Fertilizers (AREA)

Abstract

The invention discloses a special water-retaining biological organic fertilizer for blueberries and a preparation method thereof. The biological organic fertilizer is prepared from the following raw materials in parts by weight: 25-35 parts of a gelatinous microbial composition and 65-75 parts of an organic fertilizer. The total effective viable count of the special water-retaining biological organic fertilizer for the blueberries is 0.25*10<8>-0.30*10<8> cfu/g. The special water-retaining biological organic fertilizer can repeatedly release and absorb water, and the water use efficiency of the blueberries is remarkably improved, a small environment which is loose, ventilated, humid and high in organic content is provided for the roots of the blueberries, the water and nutrient absorption of the roots of the blueberries is promoted, and the yield and the quality of the blueberries are remarkably improved.

Description

Special water conservation biological organic fertilizer of blueberry and preparation method thereof
Technical field
The present invention relates to a kind of technical field of fertilizers, particularly special water conservation biological organic fertilizer of a kind of blueberry and preparation method thereof.
Background technology
Blueberry is Ericaceae (Ericaceae) Vaccinium (Vacciniumspp.) plant, is perennial undershrub seeds.Blueberry has higher nourishing function and pharmaceutical use, extremely welcome at home and abroad, and food and agricultural organization of united state is classified as one of large heath food of the mankind five.Blueberry has higher economic worth and wide DEVELOPMENT PROSPECT.In recent years, the cultivated area of blueberry is constantly expanded, and spread all over the country tens provinces and cities, and the total area is more than 3000hm 2.
The optimal soil of blueberry cultivation be loosen, good, moistening, that organic content is high acid soil of ventilating.Along with the continuous expansion of blueberry planting scale, increasing at the area of all kinds of grown on soil blueberry be not too applicable at present.In order to improve soil, generally adopting SULPHUR POWDER in blueberry cultivation and take the peat composed of rotten mosses as the co-administered method of the organic materials of representative.The strongly-acid of SULPHUR POWDER and soil makes the biological community structure of blueberry rhizosphere soil very unique, soil microorganisms utilizes the keying action in process to be difficult to realize at the humify of organic matter, mineralising and plant nutrient, be unfavorable for the normal growth of blueberry, blueberry soil microenvironment needs improvement badly.
Blueberry happiness ground moistening, in the wild range of distribution of blueberry, blueberry growth is on the organic matter with underground retention water.Under artificial cultivation condition, improve water conservation, the water-holding power of blueberry soil, be both beneficial to the normal growth of blueberry, and effectively can have reduced again the cost of man-made irrigation.
Therefore, the biological organic fertilizer with water conservation effect that development blueberry is special, stable lasting improvement blueberry soil and soil microenvironment, to improve the output of blueberry, to improve the quality of blueberry, are the active demands that current blueberry is produced.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, special water conservation biological organic fertilizer of a kind of blueberry and preparation method thereof is provided.
For achieving the above object, technical scheme of the present invention is as follows:
The special water conservation biological organic fertilizer of a kind of blueberry, is made up of the raw material of following weight parts ratio: gel microbial composite 25 ~ 35 parts, fertilizer 65 ~ 75 parts.Described blueberry special water conservation biological organic fertilizer is containing total living bacteria count 0.25 × 10 8~ 0.30 × 10 8cfu/g.
Described gel microbial composite be by microorganism adsorption liquid and absorption carrier according to (20 ~ 30): the ratio of 1 (mL/g) mixes.Described absorption carrier is CLP type braking carrier.
Described microorganism adsorption liquid by microbial composite bacterium liquid and special diluent by microbial composite bacterium liquid: special diluent=1:4 ~ 5 (V/V) mix.
Described microorganism comprises pseudomonas X123, bacillus fusiformis L13, lactobacillus johnsonii and S. cervisiae.Not antagonism between four kinds of bacterial strains, works in coordination with mutually.
The ratio of the quantity (cfu) of described pseudomonas X123, described bacillus fusiformis L13, described lactobacillus johnsonii, described S. cervisiae is: 35 ~ 45:25 ~ 35:10 ~ 20:10 ~ 20.Microbial composite bacterium liquid is containing viable count 6.0 × 10 8~ 7.0 × 10 8cfu/ml.
The pseudomonas X123 that the present invention uses, Classification And Nomenclature: first Zymomonas mobilis, Pseudomonas sp., be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 31st, 2013, deposit number is CGMCC No.7668, depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica; The bacillus fusiformis L13 that the present invention uses, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 31st, 2012, deposit number is CGMCC No.7068, open at Chinese patent 2013100166040; The S. cervisiae (saccharomyces cerevisiae) that the present invention uses, lactobacillus johnsonii (Lactobacillus casei) derive from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), S. cervisiae bacterium numbering is 2.0666, and lactobacillus johnsonii bacterium numbering is 1.0580.
The making of described special diluent: soybean sprout and water boil 10min according to the ratio of 1:30 ~ 35 (g/ml), cold filtration, obtains mixed solution, adds the glucose of 1% ~ 1.5% of mixed solution quality and the urea of 0.2% ~ 0.3% respectively, after dissolving, mixing, makes special diluent.
Described CLP type braking carrier, from Beijing Han Limiao Technew SA, size is 0.3 ~ 0.8mm.
The making of described fertilizer: chicken manure and bacterium chaff are mixed by weight 2:8, inoculate organic material composting microbial inoculum, carry out aerobic fermentation, becomes thoroughly decomposed after drying and pulverized 1 ~ 2mm sieve, water content≤15%, organic content>=60%, total nutrient N+P 2o 5+ K 2o>=5%.
Blueberry of the present invention special water conservation biological organic fertilizer preparation method, comprises the following steps:
(1) microbial composite bacterium liquid is prepared: according to the ratio of the quantity (cfu) of pseudomonas X123, bacillus fusiformis L13, lactobacillus johnsonii, S. cervisiae be: 35 ~ 45:25 ~ 35:10 ~ 20:10 ~ 20 preparing microorganism composition bacterium liquid;
(2) prepare microorganism adsorption liquid: by the microbial composite bacterium liquid in step (1) with special diluent by microbial composite bacterium liquid: special diluent=1:4 ~ 5 (V/V) mix, and make microorganism adsorption liquid;
(3) gel microbial composite is prepared: by the microorganism adsorption liquid in step (2) and absorption carrier according to (20 ~ 30): the ratio mixing of 1 (ml/g), namely forms gel microbial composite;
(4) fertilizer is prepared: by chicken manure and the mixing of bacterium chaff, obtain mixture; By described mixture inoculation organic material composting microbial inoculum, carry out aerobic fermentation, obtain fertilizer;
(5) the fertilizer mixing microbial composite obtained in step (3) and step (4) obtained, obtain mixture, mixture is pulverized aftershaping, oven drying at low temperature obtains the special water conservation biological organic fertilizer of blueberry.
Specifically comprise the following steps:
(1) microbial composite bacterium liquid is prepared
Extractum carnis 3 parts, peptone 10 parts, 5 parts, sodium-chlor, 982 parts, water are mixed and make substratum, spread cultivation pseudomonas X123, bacillus fusiformis L13 preparation pseudomonas X123 fermented liquid, bacillus fusiformis L13 fermented liquid in the medium respectively; The cultural method provided by culture presevation unit prepares the fermented liquid of yeast saccharomyces cerevisiae fermented liquid, lactobacillus johnsonii; By gained fermented liquid by the ratio of the quantity (cfu) of pseudomonas X123, bacillus fusiformis L13, lactobacillus johnsonii, S. cervisiae be: 35 ~ 45:25 ~ 35:10 ~ 20:10 ~ 20 mix preparing microorganism composition bacterium liquid; Microbial composite bacterium liquid is containing viable count 6.0 × 10 8~ 7.0 × 10 8cfu/ml.
(2) microorganism adsorption liquid is prepared
Soybean sprout and water are boiled 10min, cold filtration according to the ratio of 1:30 ~ 35 (g/ml), obtains mixed solution, add the glucose of 1% ~ 1.5% of mixed solution quality and the urea of 0.2% ~ 0.3% respectively, after dissolving, mixing, makes special diluent.
Microbial composite bacterium liquid in step (1) and special diluent are pressed microbial composite bacterium liquid: special diluent=1:4 ~ 5 (V/V) mixing, make microorganism adsorption liquid.
(3) gel microbial composite is prepared
By the microorganism adsorption liquid in step (2) and absorption carrier according to (20 ~ 30): the ratio mixing of 1 (ml/g), namely forms gel microbial composite.
(4) fertilizer is prepared
Chicken manure and bacterium chaff are mixed by weight 2:8, inoculates organic material composting microbial inoculum, carry out aerobic fermentation, become thoroughly decomposed after drying and pulverized 1 ~ 2mm sieve, water content≤15%, organic content>=60%, total nutrient N+P 2o 5+ K 2o>=5%.
(5) mixture process before granulating
The fertilizer gel microbial composite obtained in step (3) and step (4) obtained, by after parts by weight of raw materials metering, drops in mixing machine and fully mixes, obtain mixture, pulverized by mixture.
(6) granulate, dry, cool, sieve, pack
Dropped in Squeezinggranulator by the material crushed and granulate, dropped into by cylindrical particle in dryer, bake out temperature controls, below 50 DEG C, to dry to water content≤15%, then sieves packaging after cooling.
The invention has the beneficial effects as follows:
1, the present invention with pseudomonas X123, bacillus fusiformis L13 for main ingredient, and the optimum combination microbial composite bacterium liquid of other function yeast, achieve multi-functional bacterial strain combination, composite bacterial solution has stronger specific aim and validity to blueberry, significantly improves blueberry soil microenvironment.
2, CLP type braking carrier combines with composite bacterial solution by the present invention, and is aided with special diluent, ensure that activity and the stability of function yeast.
3, special water conservation biological organic fertilizer of the present invention, can emission and absorption moisture repeatedly, significantly improve the water use efficiency of blueberry, for blueberry root system provides loose, ventilative, moistening, that organic content is high subenvironment, facilitate the absorption of blueberry root system to moisture and nutrient, significantly improve the yield and quality of blueberry.
Accompanying drawing explanation
Fig. 1 shows: nutritive medium and clear water are on the impact of viable count.
Fig. 2 shows: experimental group and control group are on the impact of living bacteria count.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further described.
The preparation of the special water conservation biological organic fertilizer of embodiment 1 blueberry
Extractum carnis 3 parts, peptone 10 parts, 5 parts, sodium-chlor, 982 parts, water are mixed and make substratum, spread cultivation pseudomonas X123, bacillus fusiformis L13 preparation pseudomonas X123 fermented liquid, bacillus fusiformis L13 fermented liquid in the medium respectively;
The cultural method provided by culture presevation unit prepares the fermented liquid of yeast saccharomyces cerevisiae fermented liquid, lactobacillus johnsonii.
The number ratio of gained fermented liquid by contained viable count is mixed: the ratio of the quantity (cfu) of pseudomonas X123, bacillus fusiformis L13, lactobacillus johnsonii, S. cervisiae is: 40:30:15:15.Microbial composite bacterium liquid is 6.58 × 10 containing viable count 8cfu/ml.Soybean sprout and water are boiled 10min, cold filtration according to the ratio of 1:30 (g/ml), adds the glucose of 1% and the urea of 0.3%, after dissolving, mixing, makes special diluent.By microbial composite bacterium liquid and special diluent by microbial composite bacterium liquid: special diluent=1:5 (V/V) is mixed and made into microorganism adsorption liquid.
Microorganism adsorption liquid and CLP type are braked carrier fully mix in the ratio of 25:1 (ml/g), make gel microbial composite.
Chicken manure and bacterium chaff are mixed by weight 2:8, inoculates organic material composting microbial inoculum, carry out aerobic fermentation, become thoroughly decomposed after drying and pulverized 2mm sieve, obtained water content≤15%, organic content>=60%, total nutrient N+P 2o 5+ K 2the fertilizer of O>=5%.
After the weight part metering of gel microbial composite 25 parts, fertilizer 75 parts, drop in mixing machine and fully mix, obtain mixture, mixture is pulverized.Dropped in Squeezinggranulator by the material crushed and granulate, dropped into by cylindrical particle in dryer, bake out temperature controls, at 50 DEG C, to dry to water content≤15%, then sieves packaging after cooling.
The viable count of the special water conservation biological organic fertilizer of the blueberry made is: 0.30 × 10 8cfu/g.
Embodiment 2 is exclusively used in the microbial composite bacterium liquid of blueberry
Pseudomonas X123, bacillus fusiformis L13, lactobacillus johnsonii, S. cervisiae are pressed the ratio mixing of the cfu of 40:30:15:15, microbial composite bacterium liquid is 6.58 × 10 containing viable count 8cfu/ml.Field planting blueberry seedling, and respectively by 4 kinds of bacterium liquid and water in 1: 2 ratio mix, pour into according to the total amount of every basin 4,000,000,000 viable counts.After blueberry grows 6 months, measure blueberry rhizosphere soil physico-chemical property, and measure plant height and stem is thick, result is as shown in table 1 below.Can find out, with single inoculation pseudomonas X123, bacillus fusiformis L13, lactobacillus johnsonii, the contrast of S. cervisiae bacterium liquid phase, microbial composite bacterium liquid process rhizosphere soil bacterial number is significantly the highest, and blueberry plant height and leading thread are all significantly greater than other several process.Therefore, microbial composite bacterium liquid significantly can increase number of bacteria, promotes blueberry growth.
The impact that table 1 composition bacterium liquid grows blueberry
The test of the living bacteria count of embodiment 3 gel microbial composite
Soybean sprout and water boil 10min, cold filtration according to the ratio of 1:30 (g/ml), add the glucose of 1% and the urea of 0.3%, dissolve rear mixing, make special diluent.Pseudomonas X123, bacillus fusiformis L13, lactobacillus johnsonii, S. cervisiae are pressed the ratio mixing of the cfu of 40:30:15:15, microbial composite bacterium liquid is 6.58 × 10 containing viable count 8cfu/ml, presses 1:5 (V/V) with clear water and special diluent respectively and dilutes.Take 50g CLP type braking carrier, two kinds are fully mixed with the ratio of carrier in 25:1 (ml/g) respectively by the microbial composite bacterium liquid after diluting, makes gel microbial composite, be placed on Air drying cool place place, natural storage.Get 10g sample after carrier adsorption, measure viable count by dilution plate counting process, within later every three days, measure once.Result as shown in Figure 1.Can find out, measuring from first time, the living bacteria count of special diluted process is all significantly higher than clear water process.Therefore, special diluent process is utilized to be conducive to the survival of microorganism in bacterium liquid.
The living bacteria count test of the special water conservation biological organic fertilizer of embodiment 4 blueberry
Process 1: microbial composite bacterium liquid and special diluent are pressed microbial composite bacterium liquid: special diluent=1:5 (V/V) mixing, brake carrier with CLP type fully to mix in the ratio of 25:1 (ml/g) and make gel microbial composite, after gel microbial composite 25 parts (weight part), fertilizer 75 parts (weight part) metering, drop in mixing machine and fully mix, obtain mixture, mixture is pulverized.Dropped in Squeezinggranulator by the material crushed and granulate, dropped into by cylindrical particle in dryer, bake out temperature controls, below 50 DEG C, to dry to water content≤15%, then screening (experimental group) after cooling; By microbial composite bacterium liquid with after special diluted 5 times, the microorganism adsorption liquid of volume same with process 1 is sprayed into the fertilizer processing 1 same weight in mix thoroughly, after add weight same with process 1 again CLP type brake carrier, pulverize after mixing, be squeezed into column, temperature same with process 1 is dried to water content≤15%, then screening (control group) after cooling.Measure the viable count in microbial inoculum after natural storage 90d, result as shown in Figure 2.Can find out, the living bacteria count of experimental group is significantly greater than control group.
Blueberry special water conservation biological organic fertilizer field test
Blueberry special water conservation biological organic fertilizer field test is arranged in base, the Zang Jia village, Jia Wo group Huangdao District.Blueberry main breed is blue rich, and the age of tree is 7 years.Use the special water conservation biological organic fertilizer (experimental group) of blueberry that embodiment 3 is obtained, with fertilizer in contrast (control group).In application in two dressings in 2013: at the beginning of 3 months, every tree is used test fertilizer or contrasts fertile 5kg, every tree is used test fertilizer or contrasts fertile 5kg by the end of June, and adopt and dig ring-type ditch spread, Physicochemical fertilizer is normally used everywhere, by land for growing field crops ordinary method normal management.Measure the yield and quality index of blueberry in June, 2013, the results are shown in following table 2.As can be seen from the test results, that uses bio-organic fertilizer special significantly improves single fruit weight, improves fruit total reducing sugar and soluble solid content.Therefore, the special water conservation biological organic fertilizer of blueberry can improve blueberry output, improves fruit quality.
Table 2 biological organic fertilizer is on the impact of Blueberry yield and quality
By reference to the accompanying drawings the specific embodiment of the present invention is described although above-mentioned; but not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various amendment or distortion that creative work can make still within protection scope of the present invention.

Claims (9)

1. one kind is exclusively used in the microbial composite bacterium liquid of blueberry, it is characterized in that, comprise blueberry rhizosphere growth-promoting pseudomonas X123, blueberry rhizosphere growth-promoting bacillus fusiformis L13, lactobacillus johnsonii and S. cervisiae, the ratio of the quantity (CFU) of described blueberry rhizosphere growth-promoting pseudomonas X123, described blueberry rhizosphere growth-promoting bacillus fusiformis L13, described lactobacillus johnsonii is: 35 ~ 45:25 ~ 35:10 ~ 20:10 ~ 20, and microbial composite bacterium liquid is containing viable count 6.0 × 10 8~ 7.0 × 10 8cfu/ml.
Described blueberry rhizosphere growth-promoting pseudomonas X123, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.7668;
Described blueberry rhizosphere growth-promoting bacillus fusiformis L13, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.7068;
Described S. cervisiae is the bacterium numbering at China Committee for Culture Collection of Microorganisms's common micro-organisms center is the bacterial strain of 2.0666;
Described lactobacillus johnsonii is the bacterium numbering at China Committee for Culture Collection of Microorganisms's common micro-organisms center is the bacterial strain of 1.0580.
2. a microorganism adsorption liquid, it is characterized in that: be mixed by microbial composite bacterium liquid described in claim 1 and special diluent, described microbial composite bacterium liquid: the volume ratio of special diluent is 1:4 ~ 5, the making of described special diluent: soybean sprout and water boil 10min according to the ratio of 1:30 ~ 35 (g/ml), cold filtration, obtain mixed solution, add the glucose of 1% ~ 1.5% of mixed solution quality and the urea of 0.2% ~ 0.3% respectively, mixing after dissolving.
3. a gel microbial composite, is characterized in that: be by the microorganism adsorption liquid in claim 2 and absorption carrier according to (20 ~ 30): the ratio of 1 (V/W) mixes.
4. gel microbial composite as claimed in claim 3, is characterized in that: described absorption carrier is CLP type braking carrier.
5. the special water conservation biological organic fertilizer of blueberry, is characterized in that: be made up of the raw material of following weight parts ratio: the microbial composite of gel described in claim 3 25 ~ 35 parts, fertilizer 65 ~ 75 parts.
6. the special water conservation biological organic fertilizer of blueberry as claimed in claim 5, it is characterized in that: the making of described fertilizer: chicken manure and bacterium chaff are mixed by weight 2:8, inoculate organic material composting microbial inoculum, carry out aerobic fermentation, become thoroughly decomposed after drying and pulverized 1 ~ 2mm sieve, obtained water content≤15%, organic content>=60%, total nutrient N+P 2o 5+ K 2the fertilizer of O>=5%.
7. the special water conservation biological organic fertilizer of blueberry as claimed in claim 5, is characterized in that: the total living bacteria count 0.25 × 10 of described blueberry special water conservation biological organic fertilizer 8~ 0.30 × 10 8cfu/g.
8. a preparation method for the special water conservation biological organic fertilizer of blueberry, is characterized in that, comprise the following steps:
(1) microbial composite bacterium liquid is prepared: according to the ratio of the quantity (cfu) of pseudomonas X123, bacillus fusiformis L13, lactobacillus johnsonii, S. cervisiae be: 35 ~ 45:25 ~ 35:10 ~ 20:10 ~ 20 preparing microorganism composition bacterium liquid;
(2) prepare microorganism adsorption liquid: by the microbial composite bacterium liquid in step (1) and special diluent by microbial composite bacterium liquid: special diluent=1:4 ~ 5 (V/V) mixing, make microorganism adsorption liquid;
(3) gel microbial composite is prepared: by the microorganism adsorption liquid in step (2) and absorption carrier according to (20 ~ 30): the ratio mixing of 1 (ml/g), namely forms gel microbial composite;
(4) fertilizer is prepared: by chicken manure and the mixing of bacterium chaff, obtain mixture; By described mixture inoculation organic material composting microbial inoculum, carry out aerobic fermentation, obtain fertilizer;
(5) the fertilizer mixing microbial composite obtained in step (3) and step (4) obtained, obtain mixture, mixture is pulverized aftershaping, oven drying at low temperature obtains the special water conservation biological organic fertilizer of blueberry.
9. preparation method as claimed in claim 8, it is characterized in that, concrete steps are as follows:
(1) microbial composite bacterium liquid is prepared
Extractum carnis 3 parts, peptone 10 parts, 5 parts, sodium-chlor, 982 parts, water are mixed and make substratum, spread cultivation pseudomonas X123, bacillus fusiformis L13 preparation pseudomonas X123 fermented liquid, bacillus fusiformis L13 fermented liquid in the medium respectively; The cultural method provided by culture presevation unit prepares the fermented liquid of yeast saccharomyces cerevisiae fermented liquid, lactobacillus johnsonii; By gained fermented liquid by the ratio of the quantity (cfu) of pseudomonas X123, bacillus fusiformis L13, lactobacillus johnsonii, S. cervisiae be: 35 ~ 45:25 ~ 35:10 ~ 20:10 ~ 20 mix preparing microorganism composition bacterium liquid; Microbial composite bacterium liquid is containing viable count 6.0 × 10 8~ 7.0 × 10 8cfu/ml;
(2) microorganism adsorption liquid is prepared
Soybean sprout and water are boiled 10min, cold filtration according to the ratio of 1:30 ~ 35 (g/ml), obtains mixed solution, add the glucose of 1% ~ 1.5% of mixed solution quality and the urea of 0.2% ~ 0.3% respectively, after dissolving, mixing, makes special diluent;
Microbial composite bacterial concentration in step (1) and special diluent are pressed microbial composite bacterium liquid: special diluent=1:4 ~ 5 (V/V) mixing, make microorganism adsorption liquid;
(3) gel microbial composite is prepared
By the microorganism adsorption liquid in step (2) and absorption carrier according to (20 ~ 30): the ratio mixing of 1 (ml/g), namely forms gel microbial composite;
(4) fertilizer is prepared
Chicken manure and bacterium chaff are mixed by weight 2:8, inoculates organic material composting microbial inoculum, carry out aerobic fermentation, become thoroughly decomposed after drying and pulverized 1 ~ 2mm sieve, water content≤15%, organic content>=60%, total nutrient N+P 2o 5+ K 2o>=5%;
(5) mixture process before granulating
The fertilizer gel microbial composite obtained in step (3) and step (4) obtained, by after parts by weight of raw materials metering according to claim 4, drops in mixing machine and fully mixes, obtain mixture, pulverized by mixture;
(6) granulate, dry, cool, sieve, pack
Dropped in Squeezinggranulator by the material crushed and granulate, dropped into by cylindrical particle in dryer, bake out temperature controls, below 50 DEG C, to dry to water content≤15%, then sieves packaging after cooling.
CN201510149708.8A 2015-03-31 2015-03-31 Special water conservation biological organic fertilizer of blueberry and preparation method thereof Active CN104788177B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510149708.8A CN104788177B (en) 2015-03-31 2015-03-31 Special water conservation biological organic fertilizer of blueberry and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510149708.8A CN104788177B (en) 2015-03-31 2015-03-31 Special water conservation biological organic fertilizer of blueberry and preparation method thereof

Publications (2)

Publication Number Publication Date
CN104788177A true CN104788177A (en) 2015-07-22
CN104788177B CN104788177B (en) 2016-03-02

Family

ID=53553427

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510149708.8A Active CN104788177B (en) 2015-03-31 2015-03-31 Special water conservation biological organic fertilizer of blueberry and preparation method thereof

Country Status (1)

Country Link
CN (1) CN104788177B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109574720A (en) * 2019-01-07 2019-04-05 德州学院 A method of promoting poplar fast-growth
CN115777489A (en) * 2022-11-23 2023-03-14 山东省林业科学研究院 Biological greening method for garden engineering

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102965319A (en) * 2012-11-30 2013-03-13 山东省林业科学研究院 Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof
CN103060242A (en) * 2013-01-17 2013-04-24 山东省林业科学研究院 Bacillus fusiformis for promoting rhizosphere growth of blueberries and application thereof
CN104250127A (en) * 2014-09-09 2014-12-31 安徽省司尔特肥业股份有限公司 Fertilizer special for blueberry and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102965319A (en) * 2012-11-30 2013-03-13 山东省林业科学研究院 Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof
CN103060242A (en) * 2013-01-17 2013-04-24 山东省林业科学研究院 Bacillus fusiformis for promoting rhizosphere growth of blueberries and application thereof
CN104250127A (en) * 2014-09-09 2014-12-31 安徽省司尔特肥业股份有限公司 Fertilizer special for blueberry and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109574720A (en) * 2019-01-07 2019-04-05 德州学院 A method of promoting poplar fast-growth
CN115777489A (en) * 2022-11-23 2023-03-14 山东省林业科学研究院 Biological greening method for garden engineering

Also Published As

Publication number Publication date
CN104788177B (en) 2016-03-02

Similar Documents

Publication Publication Date Title
CN105294284B (en) A kind of preparation method of stalk fermentation bio-feritlizer
CN103964946B (en) A kind of paddy rice Special compound microbial fertilizer and preparation method thereof
CN104387136B (en) Method for producing organic fertilizer from municipal dry branch/fallen leaf waste and application thereof
CN106047764B (en) A kind of low temperature effective microorganism preparation product applying to straw-returning
CN106701095B (en) Soil conditioner and application thereof in desert control
CN106565357B (en) Method for preparing machine-transplanted rice seedling raising substrate by taking rice straw fermentation product as raw material
CN1244265C (en) Substrate for cultivating vegetable
CN102180743A (en) Method for producing insect resistance organic compound fertilizer by fermentation of agricultural organic wastes
CN100497267C (en) Process of fermenting fruit dregs and stalk to produce fulvic acid bacterial manure
CN104447073A (en) Special compound microorganism formulated fertilizer for pomegranate and preparation method and application thereof
CN103194407A (en) Straw-decomposition composite microbial preparation and preparation method thereof
CN113774050B (en) Microbial agent for promoting nutrient absorption
CN103420731A (en) Compatibility of ganoderma lucidum cultivation material and preparation method of ganoderma lucidum cultivation material
CN109536410B (en) Salt-tolerant growth-promoting composite microbial inoculum and preparation method and application thereof
CN105085121A (en) Composite microbial fertilizer
CN107721658A (en) A kind of fertilizer synergistic conditioning agent and its production method
CN105316249A (en) Bacillus subtilis, microbial agent and application of bacillus subtilis and microbial agent to fermentation compost maturity
CN109835881A (en) A kind of modification biological charcoal, charcoal base organic fertilizer and preparation method thereof and its application
CN106318884A (en) Straw organic decomposition agent and preparation method thereof
CN110791441A (en) Bacillus amyloliquefaciens and method for preparing gamma-polyglutamic acid fertilizer synergist by using same
CN104193508A (en) Method for preparing composite organic type environment-friendly seedling culture medium block
CN106866190A (en) A kind of method of utilization Chinese medicine residue organic fertilizer cultivation Chinese medicine and its application
CN111646852A (en) Efficient and environment-friendly composite biological organic fertilizer and preparation method thereof
CN103435383A (en) Special microbial fertilizer for seaside solonchak, and preparation method and application of microbial fertilizer
CN103011918A (en) Microbial organic phosphate fertilizer capable of preventing and controlling pseudo-ginseng blight

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
EXSB Decision made by sipo to initiate substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant