CN104761311A - Micro-ecologic organic fertilizer fermented from viscera of fish and shellfish and manufacturing method thereof - Google Patents
Micro-ecologic organic fertilizer fermented from viscera of fish and shellfish and manufacturing method thereof Download PDFInfo
- Publication number
- CN104761311A CN104761311A CN201510113582.9A CN201510113582A CN104761311A CN 104761311 A CN104761311 A CN 104761311A CN 201510113582 A CN201510113582 A CN 201510113582A CN 104761311 A CN104761311 A CN 104761311A
- Authority
- CN
- China
- Prior art keywords
- fish
- bacillus natto
- fermentation
- fertilizer
- internal organ
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to a micro-ecologic organic fertilizer fermented from viscera of fish and shellfish and a manufacturing method thereof and belongs to the technical field of organic fertilizers production. The method includes following steps: (1) performing high-density cultivation and high-spore-rate conversion cultivation to bacillus natto to prepare a high-efficiency fermentation agent; (2) mixing and pre-treating the viscera of fish and shellfish with certain auxiliary materials; (3) adding the fermentation agent with stirring uniformly; and (4) performing constant-temperature stack fermentation to prepare the finish product of the micro-ecologic organic fertilizer. The micro-ecologic organic fertilizer contains not less than 10% of total nutrients including N, P2O5 and K2O and not less than 45% of organic substances, and is not less than 6*10<9>/g in live bacteria number of the bacillus natto. The micro-ecologic organic fertilizer not only includes abundant nutrients required in growth of crops but also has a large amount of probiotics, bacillus natto. By means of the effects of the bacillus natto, the organic substances, which are difficult to absorb, in the soil can be decomposed to generate humus which is absorbable by crops, thereby improving soil structure, enhancing soil fertility and reducing soil bulk density. The micro-ecologic organic fertilizer can improve soil arability so that an urgent demand of green organic agriculture production can be greatly satisfied.
Description
Technical field
The present invention relates to a kind of fertilizer, be specifically related to a kind of Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment and preparation method thereof, belong to organic fertilizer production technical field.
Background technology
The agricultural modernization of China just advances towards the direction of Sustainable development, but contradiction between grain and environment is very outstanding, brings many opportunities and challenges to agricultural science and technology work.In decades, the fertilizer that enterprise produces drops into considerably less, almost maintain crop yield with " chemical fertilizer fluttering, ammoniacal liquor are watered ", cause the hardening soil, soil fertility decline, crops quality reduction, ecotope to be subject to serious destruction thus, seriously constrain China's Agro-ecology Sustainable development.Current people generally blame that undue chemical fertilizer and the agricultural chemicals of relying on causes that melon is not fragrant, fruit is sweetless, dish is tasteless, people has eaten and easily fallen ill for, and fertilizer amount increase also makes agriculture production cost more and more higher.Tiny ecosystem fertilizer is the third generation fertilizer after farm manure and chemical fertilizer, and being nontoxic free of contamination environmental protection pioneer, is the dedicated fertilizer of green food and organic Farming.The green vital role of Tiny ecosystem fertilizer shows five aspects: one is promote the nutrient needed for plant growth, the nutrient being difficult to absorb by crop in nutrient in organic fertilizer needed for plant growth or soil by microbial process extracts, and is transformed into the nutrient supply crop that crop easily absorbs; Two is improvement Soil structures, strengthens soil fertility, because the organic matter of soil is the important indicator of soil fertility, be the basic substance forming good soil environment, and the soil ulmin in organic fertilizer can become organic matter stable in soil, thus promote soil fertility; Three is that organic fertilizer produces hydroxy kind material in soil, is combined, forms coacervate with the polyvalent metal ion of soil clay particle surface or hydrogen-oxygen polymer surfaces, the soil weight can be reduced, improve soil aeration situation, tilth is improved, thus improves soil physical property; Four is that organic fertilizer dissociation in the soil solution goes out hydrogen ion, has very strong cation exchange capacity (CEC), can displace the positively charged ion in soil mineral, makes it be transformed into nutritive substance needed for crop, adds the water regain of soil, water holding capacity and water-retentivity; Five is that microorganism probiotic aprowl or after death can discharge multiple amino acids, the VITAMIN such as paddy amic acid, proline(Pro), also has the plant hormones such as phytokinin, plant hormone, Plant hormones regulators,gibberellins, can bring huge promoter action to crop growth.
China is the relatively advanced country of processing of aquatic products in the world, and be the country that a unique in the world aquaculture amount exceedes amount of fishing, cultivation mainly concentrates on fresh water field, along with the breakthrough of cultivation breeding technique, the output of sea-food was also rising year by year in recent years, and fish and shellfish processing creates a large amount of tankage, fish and shellfish internal organ are major portions of the tankage of fish and shellfish processed goods, comprise all internal organs in the fish cheek and fish belly, shellfish is Digestive tract mainly, wherein the ratio shared by internal organ of fresh-water fishes is maximum, the fresh-water fishes internal organ of cultivation account for 35% ~ 45% of fish gross weight, the shellfish that internal organ account for large percentage has abalone, scallop, belt shellfish etc., account for 10 ~ 40% of gross weight, such as certain plant produced fresh-water fishes processed goods, calculate by processing 2 tons of fishes every day, the internal organ up to 0.9 ton will be produced, if add the processing of the sea-food of 2 tons of ocean fishes and shellfish, the internal organ amount that every day produces is just close to 2 tons, if and these fish and shellfish internal organ do not obtain the development that suitable utilization directly will affect fish processing industry.More with the research report of fish viscera refinement fish oil at present, but because the cost refining fish oil is larger, and the refuse that will discharge after refinement fish oil is more, so most of fish and shellfish internal organ are all not used for refinement fish oil, but directly walk clearly as rubbish and naturally stack.Because fish and shellfish internal organ contain more protein and fat, naturally stacking digest process needs chronic, and produces corrupt foul smell, serious environment pollution.
Fish and shellfish internal organ are the fish cheek mainly, stomach, liver, intestines, digestive gland of courage and shellfish etc., some foodstuff residues do not digested or some materials to be drained in its stomach and intestine, containing more protein, fat and myofiber, and Bacillus natto (Bacillus natto) is for the production of natto food and the microbial strains as feeding additive aquatic animal, safety non-toxic, reproduction speed is fast, vitality is very strong, all fermentation can be grown 5 ~ 55 DEG C of temperature ranges, extracellular protein lytic enzyme can be secreted in process of growth, the Nattokinase (Nattokinase NK) of lipolytic enzyme and short fiber hydrolization, rapidly the fermentation of fish and shellfish internal organ can be resolved into the soil ulmin of steady state.So adopt the method for bacillus natto to ferment fish and shellfish internal organ can produce biological organic fertilizer quickly and efficiently, the product of gained is the biological organic fertilizer of Bacillus natto containing comparatively high amts and more rich organic composition simultaneously.Utilize the development of fish and shellfish internal organ ferment making Tiny ecosystem fertilizer to fish processing industry and aquaculture all extremely important, contaminate environment can not only be avoided, the added value of fish and shellfish processing can also be improved, if such as production line calculates by the fresh-water fishes of 1 ~ 3t/h, the fish viscera of 0.35 ~ 1.35t/h will be produced, namely can produce the green fertilizer about doubling dose for the fertilizer that ferments, the economic benefit brought is huge.
In recent years, the research report in fertilizer has a lot, Chinese invention " a kind of fertilizer and preparation method thereof ", and application number 201210422218.7 discloses a kind of method that ight soil, stalk, plant ash etc. make fertilizer; Chinese invention " a kind of chicken manure organic fertilizer and preparation method thereof ", application number 201410098307.X discloses a kind of method that chicken manure, natural zeolite, turfy soil etc. make fertilizer; Also the various fermenting raw materials such as useful pig manure, wormcast, tea grounds makes the research of fertilizer; Chinese invention " a kind of amendment for sludge fermentation fertilizer and method of manufacture and use thereof ", application number 201110222364.0 discloses one and utilizes bacillus natto, is made the method for fertilizer by sludge fermentation; Research in the utilizing of fish viscera also has a lot, Chinese invention " a kind of method of comprehensive utilization of fish viscera ", application number 201110309017.1 discloses a kind of method utilizing fish viscera Fodder making, but there is not yet using fish and shellfish internal organ as raw material, carry out the report of ferment making Tiny ecosystem fertilizer with Bacillus natto.
Summary of the invention
The object of this invention is to provide a kind of production method utilizing fermenting bacillus natto fish and shellfish internal organ to produce the Tiny ecosystem fertilizer containing abundant nutrient and a large amount of Bacillus natto.Be brown with the fertilizer that the method prepares, tasteless or there is light fermented soya beans, salted or other wise fragrance taste, in the indices such as total nutrient content, organic component content and probiotic bacterium content, all reach the standard of national Tiny ecosystem fertilizer.The present invention needs solution three technical problems: the manufacturing technology of (1) high-efficiency ferment; (2) temperature control technology of fertilizer stacking fermentation; (3) organic fertilizer formula composition and technological process.
For realizing the object of foregoing invention, the technical scheme that the present invention takes is as follows:
A kind of making method of the Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment, first Bacillus natto is carried out concentration cultivation, and then carry out short gemma generation cultivation, farthest to keep the quantity of Bacillus natto cell, its fermentation capacity is not weakened, then by starter obtained after its concentrate drying; By fish and shellfish internal organ after 100 DEG C of heating, puddle evenly with other auxiliary material by composition of filling a prescription, access starter carries out stacking fermentation, utilizes hygrosensor to monitor stack center temperature in main fermentation stage, carry out when temperature is too high turning over buttress with heat radiation, prevent temperature too high; Until fermentation base-material is made particulate state with tablets press after fermentation ends, finally carry out drying, specifically comprise the steps:
(1) high-density culture of Bacillus natto:
Utilize inorganic salt, C source and N source are the substratum of raw material composition, by the method for liquid three grades of seed culture, in 37 DEG C, pH is constant temperature culture under the condition of 7.0, one-level is cultivated: the Bacillus natto that liquid state activates is inoculated in 100mL triangular flask substratum, namely primary seed solution is obtained after 46 ~ 50 hours, then triangular flask first order seed nutrient solution being inoculated in 1000mL is placed in shaking table and cultivates, secondary seed solution is obtained after 46 ~ 50 hours, again secondary seed solution access is cultivated 22 ~ 26 hours with in the 10L capacity fermentor tank of agitator, namely three grades of seed liquor are obtained, every grade of seed inoculum size is 10%, period keeps good ventilation condition, turn out Bacillus natto high-density cells liquid,
(2) short cell forms the cultivation of gemma:
The calcium of cultured Bacillus natto high-density cells liquid interpolation 0.1 ~ 0.3%, magnesium, zinc inorganic ion are carried out induction gemma and generate cultivation, and adopt the temperature stress CMC model method of 20 DEG C or 45 DEG C, to impel gemma to generate, incubation time is 22 ~ 26 hours simultaneously;
(3) starter dried recovered:
Utilize multi-stage type reliever or common reliever by the process of nutrient solution evaporation concentration, then carry out drying by spray-drying process, inlet temperature controls at 50 DEG C, and air outlet temperature controls, at 40 DEG C, to obtain bacillus natto to ferment agent after packaging;
(4) fish and shellfish internal organ pre-treatment:
Because the water content of fish and shellfish internal organ is higher, large about about 60-70%, compare thickness simultaneously, be unfavorable for fermenting bacillus natto, after fish and shellfish heating viscera to 100 DEG C is maintained 35 ~ 45 minutes, add conditioning material:, resinous shale soil, broken tangerine bar, sawdust, tealeaf residue or the dregs of a decoction, stir, moisture is reduced to 35 ~ 40%, makes base-material loosen simultaneously, obtain the base-material that ferments;
(5) inoculating starter ferments:
Fermentation base-material in add weight percent be 5 ~ 10% starter stir, enter fermentation stage, whole fermentation stage is divided into Primary Fermentation and secondary fermentation two benches, main fermentation stage piles slivering buttress by after the agent of fish and shellfish internal organ inoculation fermenting bacillus natto, buttress material is stirred once with turnover throwing machine every 2 ~ 4 days, the heat that fermenting bacillus natto is produced distributes in time, control leavening temperature 30 ~ 50 DEG C, because the too high growth being unfavorable for Bacillus natto of temperature, stir the oxygen that buttress expects can provide needed for Bacillus natto growth and breeding simultaneously, also buttress material will be stirred when the base temperature that ferments is too high, make it radiating and cooling, this process is 4 ~ 7 days, then the secondary fermentation stage is entered, this stage is that the base-material after Primary Fermentation being completed is stacked to 3 meters high, any wide and long bar buttress, this process is 2 ~ 4 days, and make becoming thoroughly decomposed of fermentation base-material further, now the organic composition of fish and shellfish internal organ is fully decomposed, become stable in properties, promote the soil ulmin of soil fertility, have the Bacillus natto of some amount, the protein content that main fermentation stage terminates in rear base-material reduces more than 80%, and the secondary fermentation stage terminates rear protein residues rate≤8% simultaneously,
(6) the shaping and packaging of finished product fertilizer:
The complete fertilizer tablets press of fermentation is carried out shaping making, and making particle diameter is the cylindrical of 5 ~ 10mm or circular granular, or makes loose powder type, then uses 45 ~ 55 DEG C of warm air dryings to moisture content to be less than 8%, and packaging is finished product.
Fertilizer making method of the present invention, requires to carry out in the factory building meeting hygienic standard.
High-density culture described in step (1) is final nutrient solution cell count>=10 after referring to and cultivating
8mL
-1.
Liquid described in step (1) three grades of seed culture substratum used is: primary-seed medium is (g L
-1): soy peptone 10, extractum carnis 5, glucose 15, NaCl 5; Secondary seed medium is (g L
-1): soy peptone 20, glucose 15, extractum carnis 10, NaCl 5; Three grades of seed culture mediums are (g L
-1): soy peptone 40, soybean meal 50, glucose 30, extractum carnis 20, NaCl 15, K
2hPO
42.0, KH
2pO
44.0, MgSO
40.5, CaCl
20.25.
The method of the liquid three grades of seed culture described in step (1) is: one-level is cultivated: be inoculated in the primary-seed medium of 100mL by the Bacillus natto that liquid state activates, namely primary seed solution is obtained after 48 hours, then first order seed nutrient solution is inoculated in the secondary seed medium of 1000mL, be placed in shaking table to cultivate, secondary seed solution is obtained after 48 hours, again secondary seed solution access is cultivated 24 hours with in the 10L capacity fermentor tank of agitator, namely obtain three grades of seed liquor, every grade of seed inoculum size is 10%.
In oscillation frequency be 250r min to keep good ventilation condition to refer to during described in step (1)
-1shaking table on cultivate.
Inorganic ion described in step (2) is CaCl
2, MgCl
2, ZnSO
4plurality of inorganic salt combines, and combination weight is than being 2:3:3.
The consumption of the fish and shellfish internal organ described in step (4) is 40 ~ 60% of fermentation base-material total amount.
The add-on of step (4) described sawdust is 5 ~ 15% of base-material amount of always fermenting, and water content is 3 ~ 5%; The add-on of described resinous shale soil is 10 ~ 15% of base-material amount of always fermenting, and water content is 5 ~ 8%; The add-on of described broken straw rod is 10 ~ 15% of base-material amount of always fermenting, and water content is 5 ~ 10%, and chip size is: maximum length≤10mm; The add-on of described tealeaf residue or the dregs of a decoction is 10 ~ 15% of base-material amount of always fermenting, and water content is 3 ~ 5%.
Utilizing the Tiny ecosystem fertilizer that fish and shellfish internal organ ferment, is the fertilizer adopting aforesaid method to be prepared from.
The present invention with fish and shellfish internal organ be main raw material, with Bacillus natto for starter.Fish and shellfish internal organ are main tankage of fish and shellfish processing, and its moisture content is comparatively large, and compares thickness, is unfavorable for fermenting bacillus natto, needs to add suitable auxiliary material, to reach the moisture content and short texture that reduce fermentation base-material, are easy to the fermentation of Bacillus natto.The relevant fertilizer of retrieval makes auxiliary material used, generally all adopt weathered coal, plant ash, broken straw rod, tealeaf residue, Chinese medicine slag, resinous shale soil, sawdust and culture medium of edible fungus waste material etc., consider that broken straw rod, resinous shale soil, tealeaf residue and sawdust easily obtain, therefore, the present invention adopts this few material as auxiliary material, efficiently solves the problem that fish and shellfish internal organ are not easy to fermenting bacillus natto; Bacillus natto is for the production of natto food and the microorganism as aquatic animal feed additive, make fertilizer and need high efficiency fermenting bacillus natto agent, namely need containing the larger starter of Bacillus natto cell count, to shorten fermentation period, give fertilizer larger Bacillus natto cell content simultaneously.Show according to science popularization data: the vitality of Bacillus natto is stronger, all can grow at 5 DEG C ~ 55 DEG C, and the Bacillus natto vitality of gemma state is stronger than nourishing body, still can survive at 100 DEG C of temperature, therefore, the present invention still can keep higher activity to make Bacillus natto in the technological process of heat drying, utilizes concentration cultivation and short gemma to generate culture technique, efficiently solves the problem of the technology of high-activity fermented dose of this key.
The invention has the beneficial effects as follows: the present invention is after fish and shellfish internal organ are carried out heating deinsectization and harmful bacteria process, add auxiliary material allotment, then access the fermenting bacillus natto agent made in advance, make green ecological fertilizer afterwards by fermentation.Whole process does not add any material HUMAN HEALTH being had to harm, microorganism Bacillus natto used of fermenting is safe and harmless probiotic bacterium, fish and shellfish internal organ contain the organic compositions such as higher myofiber, protein and fat, after fermenting bacillus natto, generate the soil ulmin of stable in properties, do not produce stink in production process, finished product fertilizer not only fertility enriches, and a large amount of Bacillus nattos contained, can strengthen soil fertility, reduce the use of fertilizer amount and agricultural chemicals, be the excellent fertilizer that green agriculture is produced.This Tiny ecosystem fertilizer contains total nutrient (N+P2O5+K2O)>=10%, organic composition>=45%, Bacillus natto viable count>=6 × 10
9individual/g, not only have and enrich nutrient needed for plant growth, also there is a large amount of probiotic bacteriums: Bacillus natto, the organic composition being difficult to absorb by crop in soil by the effect of Bacillus natto decomposes, generate the absorbent soil ulmin of crop, thus the object reaching improvement Soil structure, strengthen soil fertility, reduce the soil weight, soil tilth is improved, greatly meets the active demand of the green organic agricultural production that current people pursue.
Embodiment
Be described in further details the present invention below by example, these examples are only used for the present invention is described, do not limit the scope of the invention.
Embodiment 1
A making method for the Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment, comprises the steps:
(1) high-density culture of Bacillus natto:
Utilize inorganic salt, C source and N source are the substratum of raw material composition, by the method for liquid three grades of seed culture, in 37 DEG C, pH is constant temperature culture under the condition of 7.0, one-level is cultivated: the Bacillus natto that liquid state activates is inoculated in 100mL triangular flask substratum, namely primary seed solution is obtained after 48 hours, then triangular flask first order seed nutrient solution being inoculated in 1000mL is placed in shaking table and cultivates, secondary seed solution is obtained after 48 hours, again secondary seed solution access is cultivated 24 hours with in the 10L capacity fermentor tank of agitator, namely three grades of seed liquor are obtained, every grade of seed inoculum size is 10%, period keeps good ventilation condition, be 250r min in oscillation frequency
-1shaking table in cultivate, turn out Bacillus natto high-density cells liquid, final nutrient solution cell count>=10 after cultivating
8mL
-1,
The primary-seed medium that three grades of described seed culture are used is (g L
-1): soy peptone 10, extractum carnis 5, glucose 15, NaCl5; Secondary seed medium is (g L
-1): soy peptone 20, glucose 15, extractum carnis 10, NaCl 5; Three grades of seed culture mediums are (g L
-1): soy peptone 40, soybean meal 50, glucose 30, extractum carnis 20, NaCl 15, K
2hPO
42.0, KH
2pO
44.0, MgSO
40.5, CaCl
20.25.
(2) short cell forms the cultivation of gemma:
Cultured Bacillus natto high-density cells liquid is added complex inorganic salt catalyst (g L
-1): CaCl 0.2, MgCl
20.3, ZnSO
40.3, carry out induction gemma and generate cultivation, adopt the temperature stress CMC model method of 20 DEG C or 45 DEG C, to impel gemma to generate, the constant temperature culture time is 24 hours simultaneously;
(3) starter dried recovered:
The liquid Bacillus natto reduction vaporization method of enrichment of having cultivated carry out concentrated after, then carry out drying by spray-drying process, inlet temperature controls at 50 DEG C, and air outlet temperature controls, at 40 DEG C, to obtain bacillus natto to ferment agent for subsequent use after packaging;
(4) fish and shellfish internal organ pre-treatment:
Because the water content of fish and shellfish internal organ is higher, large about about 60-70%, compare thickness simultaneously, be unfavorable for fermenting bacillus natto, after fish and shellfish heating viscera to 100 DEG C is maintained 40 minutes, add conditioning material: resinous shale soil 10%, broken tangerine bar 10%, sawdust 15%, tealeaf residue 15% stir with fish and shellfish internal organ 50%, and moisture is reduced to 35%, make base-material loosen simultaneously, obtain the base-material that ferments;
(5) inoculating starter ferments:
Fermentation base-material in add weight percent be 10% starter stir, enter fermentation stage, whole fermentation stage is divided into Primary Fermentation and secondary fermentation two benches, main fermentation stage is stacked to high 1m by after the agent of fish and shellfish internal organ inoculation fermenting bacillus natto, the bar buttress of wide 3m, buttress material is stirred once with turnover throwing machine every 3 days, the heat that fermenting bacillus natto is produced distributes in time, control leavening temperature and be no more than 50 DEG C, because the too high growth being unfavorable for Bacillus natto of temperature, stir the oxygen that buttress expects can provide needed for Bacillus natto growth and breeding simultaneously, also buttress material will be stirred when the base temperature that ferments is too high, make it radiating and cooling, this process is 5 days, then the secondary fermentation stage is entered, this stage be by Primary Fermentation after base-material be stacked to 3 meters high, any wide and long bar buttress, this process is 3 days, and make becoming thoroughly decomposed of fermentation base-material further, now the organic composition of fish and shellfish internal organ is fully decomposed, become stable in properties, promote the soil ulmin of soil fertility, have the Bacillus natto of some amount, the protein content that main fermentation stage terminates in rear base-material reduces more than 80%, and the secondary fermentation stage terminates rear protein residues rate≤8% simultaneously,
(6) the shaping and packaging of finished product fertilizer:
The complete fertilizer tablets press of fermentation is carried out shaping making, and making particle diameter is the cylindrical of 8mm, is then less than 8% with 50 DEG C of warm air dryings to moisture content, packs and is finished product.
Embodiment 2
A making method for the Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment, comprises the steps:
(1) high-density culture of Bacillus natto:
Utilize inorganic salt, C source and N source are the substratum of raw material composition, by the method for liquid three grades of seed culture, in 37 DEG C, pH is constant temperature culture under the condition of 7.0, one-level is cultivated: the Bacillus natto that liquid state activates is inoculated in 100mL triangular flask substratum, namely primary seed solution is obtained after 46 hours, then triangular flask first order seed nutrient solution being inoculated in 1000mL is placed in shaking table and cultivates, secondary seed solution is obtained after 46 hours, again secondary seed solution access is cultivated 22 hours with in the 10L capacity fermentor tank of agitator, namely three grades of seed liquor are obtained, every grade of seed inoculum size is 10%, period keeps good ventilation condition, be 250r min in oscillation frequency
-1shaking table in cultivate, turn out Bacillus natto high-density cells liquid, final nutrient solution cell count>=10 after cultivating
8mL
-1,
The primary-seed medium that three grades of described seed culture are used is (g L
-1): soy peptone 10, extractum carnis 5, glucose 15, NaCl 5; Secondary seed medium is (g L
-1): soy peptone 20, glucose 15, extractum carnis 10, NaCl 5; Three grades of seed culture mediums are (g L
-1): soy peptone 40, soybean meal 50, glucose 30, extractum carnis 20, NaCl 15, K
2hPO
42.0, KH
2pO
44.0, MgSO
40.5, CaCl
20.25.
(2) short cell forms the cultivation of gemma:
Cultured Bacillus natto high-density cells liquid is added complex inorganic salt catalyst (g L
-1): CaCl 0.2, MgCl
20.3, ZnSO
40.3, carry out induction gemma and generate cultivation, adopt the temperature stress CMC model method of 20 DEG C or 45 DEG C, to impel gemma to generate, the constant temperature culture time is 22 hours simultaneously;
(3) starter dried recovered:
The liquid Bacillus natto reduction vaporization method of enrichment of having cultivated carry out concentrated after, then carry out drying by spray-drying process, inlet temperature controls at 50 DEG C, and air outlet temperature controls, at 40 DEG C, to obtain fermenting bacillus natto agent for subsequent use after packaging;
(4) fish and shellfish internal organ pre-treatment:
Because the water content of fish and shellfish internal organ is higher, large about about 60-70%, compare thickness simultaneously, be unfavorable for fermenting bacillus natto, after fish and shellfish heating viscera to 100 DEG C is maintained 35 minutes, add conditioning material: resinous shale soil 10%, broken tangerine bar 10%, sawdust 5%, tealeaf residue 15% stir with fish and shellfish internal organ 60%, and moisture is reduced to 35%, make base-material loosen simultaneously, obtain the base-material that ferments;
(5) inoculating starter ferments:
Fermentation base-material in add weight percent be 5% starter stir, enter fermentation stage, whole fermentation stage is divided into Primary Fermentation and secondary fermentation two benches, main fermentation stage is stacked to high 1m by after the agent of fish and shellfish internal organ inoculation fermenting bacillus natto, the bar buttress of wide 3m, buttress material is stirred once with turnover throwing machine every 2 days, the heat that fermenting bacillus natto is produced distributes in time, control leavening temperature and be no more than 50 DEG C, because the too high growth being unfavorable for Bacillus natto of temperature, stir the oxygen that buttress expects can provide needed for Bacillus natto growth and breeding simultaneously, also buttress material will be stirred when the base temperature that ferments is too high, make it radiating and cooling, this process is 4 days, then the secondary fermentation stage is entered, this stage be by Primary Fermentation after base-material be stacked to 3 meters high, any wide and long bar buttress, this process is 2 days, and make becoming thoroughly decomposed of fermentation base-material further, now the organic composition of fish and shellfish internal organ is fully decomposed, become stable in properties, promote the soil ulmin of soil fertility, have the Bacillus natto of some amount, the protein content that main fermentation stage terminates in rear base-material reduces more than 80%, and the secondary fermentation stage terminates rear protein residues rate≤8% simultaneously,
(6) the shaping and packaging of finished product fertilizer:
The complete fertilizer tablets press of fermentation being carried out shaping making, makes the circular granular that particle diameter is 5mm, is then less than 8% with 45 DEG C of warm air dryings to moisture content, packs and is finished product.
Embodiment 3
A making method for the Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment, comprises the steps:
(1) high-density culture of Bacillus natto:
Utilize inorganic salt, C source and N source are the substratum of raw material composition, by the method for liquid three grades of seed culture, in 37 DEG C, pH is constant temperature culture under the condition of 7.0, one-level is cultivated: the Bacillus natto that liquid state activates is inoculated in 100mL triangular flask substratum, namely primary seed solution is obtained after 50 hours, then triangular flask first order seed nutrient solution being inoculated in 1000mL is placed in shaking table and cultivates, secondary seed solution is obtained after 50 hours, again secondary seed solution access is cultivated 26 hours with in the 10L capacity fermentor tank of agitator, namely three grades of seed liquor are obtained, every grade of seed inoculum size is 10%, period keeps good ventilation condition, be 250r min in oscillation frequency
-1shaking table in cultivate, turn out Bacillus natto high-density cells liquid, final nutrient solution cell count>=10 after cultivating
8mL
-1,
The primary-seed medium that three grades of described seed culture are used is (g L
-1): soy peptone 10, extractum carnis 5, glucose 15, NaCl 5; Secondary seed medium is (g L
-1): soy peptone 20, glucose 15, extractum carnis 10, NaCl 5; Three grades of seed culture mediums are (g L
-1): soy peptone 40, soybean meal 50, glucose 30, extractum carnis 20, NaCl 15, K
2hPO
42.0, KH
2pO
44.0, MgSO
40.5, CaCl
20.25.
(2) short cell forms the cultivation of gemma:
Cultured Bacillus natto high-density cells liquid is added complex inorganic salt catalyst (g L
-1): CaCl 0.2, MgCl
20.3, ZnSO
40.3, carry out induction gemma and generate cultivation, adopt the temperature stress CMC model method of 20 DEG C or 45 DEG C, to impel gemma to generate, the constant temperature culture time is 26 hours simultaneously;
(3) starter dried recovered:
The liquid Bacillus natto reduction vaporization method of enrichment of having cultivated carry out concentrated after, then carry out drying by spray-drying process, inlet temperature controls at 50 DEG C, and air outlet temperature controls, at 40 DEG C, to obtain bacillus natto to ferment agent for subsequent use after packaging;
(4) fish and shellfish internal organ pre-treatment:
Because the water content of fish and shellfish internal organ is higher, large about about 60-70%, compare thickness simultaneously, be unfavorable for fermenting bacillus natto, after fish and shellfish heating viscera to 100 DEG C is maintained 45 minutes, add conditioning material: resinous shale soil 15%, broken tangerine bar 15%, sawdust 15%, tealeaf residue 15% stir with fish and shellfish internal organ 40%, and moisture is reduced to 40%, make base-material loosen simultaneously, obtain the base-material that ferments;
(5) inoculating starter ferments:
Fermentation base-material in add weight percent be 10% starter stir, enter fermentation stage, whole fermentation stage is divided into Primary Fermentation and secondary fermentation two benches, main fermentation stage is stacked to high 1m by after the agent of fish and shellfish internal organ inoculation fermenting bacillus natto, the bar buttress of wide 3m, buttress material is stirred once with turnover throwing machine every 4 days, the heat that fermenting bacillus natto is produced distributes in time, control leavening temperature and be no more than 50 DEG C, because the too high growth being unfavorable for Bacillus natto of temperature, stir the oxygen that buttress expects can provide needed for Bacillus natto growth and breeding simultaneously, also buttress material will be stirred when the base temperature that ferments is too high, make it radiating and cooling, this process is 7 days, then the secondary fermentation stage is entered, this stage is that the base-material after Primary Fermentation being completed is stacked to 3 meters high, any wide and long bar buttress, this process is 4 days, and make becoming thoroughly decomposed of fermentation base-material further, now the organic composition of fish and shellfish internal organ is fully decomposed, become stable in properties, promote the soil ulmin of soil fertility, have the Bacillus natto of some amount, the protein content that main fermentation stage terminates in rear base-material reduces more than 80%, and the secondary fermentation stage terminates rear protein residues rate≤8% simultaneously,
(6) the shaping and packaging of finished product fertilizer:
The complete fertilizer tablets press of fermentation is carried out shaping making, and making particle diameter is the cylindrical of 10mm, is then less than 8% with 55 DEG C of warm air dryings to moisture content, packs and is finished product.
Embodiment 4
A making method for the Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment, comprises the steps:
The making of fermenting bacillus natto agent: first make substratum, after Bacillus natto is activated, by three grades of seed culture methods, at 37 DEG C, 250r min
-1after being cultured to secondary seed maturation in the shaking table of oscillation frequency, the fermentor tank being transferred to 5L or 10L carries out third stage culture, makes three grades of seed liquor Bacillus natto total cellular score>=10 after cultivation
8ml
-1, then add complex inorganic salt catalyst (g L
-1): CaCl 0.2, MgCl
20.3, ZnSO
40.3, be placed in 20 DEG C or 40 DEG C and cultivate after 24 hours, after concentrated with rotatory evaporator, then be dried to pulverous fermenting bacillus natto agent with spray-drier.
Fish and shellfish internal organ have pre-treatment: heated 40 minutes at 100 DEG C of temperature by fish and shellfish internal organ, then add broken straw rod 10% by weight, tealeaf residue 10%, resinous shale soil 15%, sawdust 10% mix with fish and shellfish internal organ 55%, be cooled to less than 40 DEG C.
Inoculating starter stacking ferments: fish and shellfish internal organ good for pre-treatment fermentation base-material is accessed starter 8% by volume, turn and pile high 1 meter, wide 1.5 meters bar buttress evenly and ferment, every the temperature of 10 hours with hygrosensor detection fermentation center, if reach more than 50 DEG C to turn over buttress immediately once, make the fermentation base-material radiating and cooling at center, provide oxygen to Bacillus natto simultaneously.
(1) preparation of substratum and actication of culture: the liquid activation medium of preparation Bacillus natto and three grades of enlarged culturing substratum, then Bacillus natto is inoculated in activation culture and cultivates 48 hours based in the shaking culture case of 37 DEG C, oscillation frequency is 250r min
-1;
(2) Bacillus natto enlarged culturing: the triangular flask substratum that Bacillus natto activation culture completed is inoculated in 100mL carries out first order seed and cultivates 48 hours, rear access 500mL triangular flask substratum carries out secondary seed cultivation, three grades of seed culture mediums are accessed after 48 hours, proceed to 5L fermentor cultivation 48 hours, culture condition is the same.
(3) short gemma generates and cultivates: by complex inorganic salt catalyst: CaCl, MgCl
2, ZnSO
4make the mixed aqueous solution of 1% by weight 2 ︰ 3 ︰ 3, after sterilizing in 120 DEG C, 15 minutes, be cooled to normal temperature, then add in three grades of seed culture fluids, be placed in 40 DEG C, shaking culture case and cultivate 24 hours.
(4) starter dry packing: the nutrient solution rotatory evaporator after short gemma is cultivated carry out concentrated after, use spray-drier spraying dry, inlet temperature 50 DEG C, air outlet temperature 40 DEG C, then for subsequent use after packaging.
(5) to ferment the pre-treatment of base-material: the process such as tealeaf residue, sawdust, resinous shale soil and broken straw rod first do drying, shred, fish and shellfish heating viscera is maintained 40 minutes to 100 DEG C simultaneously, then these materials are pressed the fresh material of formula rate fish and shellfish internal organ 600kg(), broken straw rod 100g, tealeaf residue 100g, sawdust 50g, resinous shale soil 100 ~ 150g, mix, then detect the total protein content of fermentation base-material.
(6) ferment stacking: access fermenting bacillus natto agent 60g when fermentation base-material being cooled to about 37 DEG C, inoculum size is 8% of fermentation binder volume amount, 1 meter high is piled after stirring, the bar buttress of 1.5 meters wide, fermentation 4-7 days, measures the temperature of stack center every 10 hours hygrosensor, if when temperature reaches 50 DEG C or more, buttress radiating treatment must be turned over, also will turn over buttress once every three days in addition, to provide the oxygen needed for fermenting bacillus natto.Detected the content of fermentation base-material protein close to about 4 days, when protein reduction reaches more than 80%, can Primary Fermentation be terminated.
(7) secondary fermentation and finished product fertilizer shaping: base-material Primary Fermentation completed piles high 3 meters, and the bar of wide 3 meters buttress, ferment after 3 days, after forming machine granulation, carry out 50 DEG C of warm air dryings, is finished product after packing labeling.
Embodiment 5
(1) preparation and the actication of culture of substratum, Bacillus natto enlarged culturing are identical with example 4;
(2) do not add inorganic salt, directly carry out short gemma with 45 DEG C of stress conditions and generate cultivation;
(3) all the other steps are with method and fill a prescription identical with embodiment 4.
Embodiment 6
(1) to generate the steps such as cultivations, starter dry packing, the pre-treatment of fermentation base-material, ferment stacking, secondary fermentation and finished product fertilizer be shaping identical with embodiment 4 for preparation and the actication of culture of substratum, Bacillus natto enlarged culturing, short gemma;
(2) formula is (in 1kg finished product fertilizer):
The fresh material of fish and shellfish internal organ 400g(, water content about 55%), broken straw rod 150g, tealeaf residue 150g, sawdust 100 ~ 150g, resinous shale soil 100g, fermenting bacillus natto agent 60g;
(3) all the other steps are identical with embodiment 4 with method.
Claims (9)
1. a making method for the Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment, is characterized in that: comprise the steps:
(1) Bacillus natto Bacillus natto high-density culture:
By the traditional liquid three grades of seed culture methods of Bacillus natto, in 37 DEG C, pH be the condition of 7.0 under constant temperature culture, every grade of seed culture 46 ~ 50 hours, period keeps good ventilation condition, turns out Bacillus natto high-density cells liquid;
(2) Bacillus natto cell forms gemma cultivation fast:
The calcium of enchylema quality 0.1 ~ 0.3%, magnesium, zinc inorganic ion are added in cultured Bacillus natto high-density cells liquid, then carry out induction gemma and generate cultivation, adopt the temperature stress CMC model method of 20 DEG C or 45 DEG C simultaneously, to impel gemma to generate, temperature stress incubation time is 22 ~ 26 hours;
(3) preparation of starter:
The liquid Bacillus natto nutrient solution multi-stage type reliever or common reliever reduction vaporization that complete the cultivation of short cell formation gemma are concentrated, carry out drying by spray-drying process again, packaging, inlet temperature controls at 50 DEG C, air outlet temperature controls, at 40 DEG C, to obtain bacillus natto to ferment agent;
(4) fish and shellfish internal organ pre-treatment:
After fish and shellfish heating viscera to 80 ~ 120 DEG C maintain 35 ~ 45 minutes, add auxiliary material: resinous shale soil, broken tangerine bar, sawdust, tealeaf residue or the dregs of a decoction, stir, obtain fermentation base-material, the water content of fermentation base-material is 35 ~ 40%;
(5) inoculating starter ferments:
Fermentation base-material in add weight percent be 5 ~ 10% starter stir, enter fermentation stage, whole fermentation stage is divided into Primary Fermentation and secondary fermentation two benches, main fermentation stage is by stacking after the agent of fish and shellfish internal organ inoculation bacillus natto to ferment, buttress material is stirred once with turnover throwing machine every 2 ~ 4 days, control leavening temperature 30 ~ 50 DEG C, make it radiating and cooling, this process is 4 ~ 7 days; Then enter the secondary fermentation stage, this stage be by Primary Fermentation after base-material stacking, this process is 2 ~ 4 days, makes becoming thoroughly decomposed of fermentation base-material further;
(6) the shaping and packaging of finished product fertilizer:
The complete fertilizer tablets press of fermentation is carried out shaping making, and making particle diameter is the cylindrical of 5 ~ 10mm or circular granular, or makes loose powder type, then uses 45 ~ 55 DEG C of warm air dryings to moisture content to be less than 8%, and packaging is finished product.
2. the making method of a kind of Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment according to claim 1, is characterized in that: the high-density culture described in step (1) is final nutrient solution cell count>=10 after referring to and cultivating
8mL
-1.
3. the making method of a kind of Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment according to claim 1, is characterized in that: the three grades of seed culture of the liquid described in step (1) substratum used is: primary-seed medium is (g L
-1): soy peptone 10, extractum carnis 5, glucose 15, NaCl 5; Secondary seed medium is (g L
-1): soy peptone 20, glucose 15, extractum carnis 10, NaCl 5; Three grades of seed culture mediums are (g L
-1): soy peptone 40, soybean meal 50, glucose 30, extractum carnis 20, NaCl 15, K
2hPO
42.0, KH
2pO
44.0, MgSO
40.5, CaCl
20.25.
4. the making method of a kind of Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment according to claim 1, it is characterized in that: the method for the liquid three grades of seed culture described in step (1) is: one-level is cultivated: the Bacillus natto that liquid state activates is inoculated in the primary-seed medium of 100mL, namely primary seed solution is obtained after 48 hours, then first order seed nutrient solution is inoculated in the secondary seed medium of 1000mL, be placed in shaking table to cultivate, secondary seed solution is obtained after 48 hours, again secondary seed solution access is cultivated 24 hours with in the 10L capacity fermentor tank of agitator, namely three grades of seed liquor are obtained, every grade of seed inoculum size is 10%.
5. the making method of a kind of Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment according to claim 1, is characterized in that: in oscillation frequency be 250r min to keep good ventilation condition to refer to during described in step (1)
-1shaking table on cultivate.
6. the making method of a kind of Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment according to claim 1, is characterized in that: the inorganic ion described in step (2) is CaCl
2, MgCl
2, ZnSO
4plurality of inorganic salt combines, and weight percent is 2:3:3.
7. the making method of a kind of Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment according to claim 1, is characterized in that: the consumption of the fish and shellfish internal organ described in step (4) is 40 ~ 60% of fermentation base-material total amount.
8. the making method of a kind of Tiny ecosystem fertilizer utilizing fish and shellfish internal organ to ferment according to claim 1, is characterized in that: the add-on of step (4) described sawdust is 5 ~ 15% of base-material amount of always fermenting is that water content is 3 ~ 5%; The add-on of described resinous shale soil is 10 ~ 15% of base-material amount of always fermenting, and water content is 5 ~ 8%; The add-on of described broken straw rod is 10 ~ 15% of base-material amount of always fermenting, and water content is 5 ~ 10%, and chip size is: maximum length≤10mm; The add-on of described tealeaf residue or the dregs of a decoction is 10 ~ 15% of base-material amount of always fermenting, and water content is 3 ~ 5%.
9. utilize the Tiny ecosystem fertilizer that fish and shellfish internal organ ferment, it is characterized in that: described Tiny ecosystem fertilizer adopts method described in claim 1 ~ 8 any one claim to prepare.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510113582.9A CN104761311A (en) | 2015-03-16 | 2015-03-16 | Micro-ecologic organic fertilizer fermented from viscera of fish and shellfish and manufacturing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510113582.9A CN104761311A (en) | 2015-03-16 | 2015-03-16 | Micro-ecologic organic fertilizer fermented from viscera of fish and shellfish and manufacturing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104761311A true CN104761311A (en) | 2015-07-08 |
Family
ID=53643472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510113582.9A Pending CN104761311A (en) | 2015-03-16 | 2015-03-16 | Micro-ecologic organic fertilizer fermented from viscera of fish and shellfish and manufacturing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104761311A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0264084A (en) * | 1988-08-29 | 1990-03-05 | Renge:Kk | Fermented fertilizer and packaging tool of plant for culture |
| CN1156134A (en) * | 1996-01-29 | 1997-08-06 | 郭振乾 | Method for producing special organic fertilizer with soil improvement function |
| JP2004299949A (en) * | 2003-03-31 | 2004-10-28 | Kankyo Soken Kk | Composting method by burying fishery residue in soil |
| CN102173910A (en) * | 2011-03-17 | 2011-09-07 | 李大鹏 | Marine byproduct fermentation concentration composite fertilizer and production method thereof |
| CN102524531A (en) * | 2010-12-13 | 2012-07-04 | 青岛中仁药业有限公司 | Preparation method of bacillus natto solid microbial ecological agent |
| CN102964153A (en) * | 2012-11-07 | 2013-03-13 | 无锡保利化肥有限公司 | Biochemical compound fertilizer and preparation method thereof |
-
2015
- 2015-03-16 CN CN201510113582.9A patent/CN104761311A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0264084A (en) * | 1988-08-29 | 1990-03-05 | Renge:Kk | Fermented fertilizer and packaging tool of plant for culture |
| CN1156134A (en) * | 1996-01-29 | 1997-08-06 | 郭振乾 | Method for producing special organic fertilizer with soil improvement function |
| JP2004299949A (en) * | 2003-03-31 | 2004-10-28 | Kankyo Soken Kk | Composting method by burying fishery residue in soil |
| CN102524531A (en) * | 2010-12-13 | 2012-07-04 | 青岛中仁药业有限公司 | Preparation method of bacillus natto solid microbial ecological agent |
| CN102173910A (en) * | 2011-03-17 | 2011-09-07 | 李大鹏 | Marine byproduct fermentation concentration composite fertilizer and production method thereof |
| CN102964153A (en) * | 2012-11-07 | 2013-03-13 | 无锡保利化肥有限公司 | Biochemical compound fertilizer and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王猛: "饲用纳豆芽孢杆菌浓缩菌粉生产工艺研究", 《中国优秀硕士学位论文全文数据库（电子期刊）农业科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104529573B (en) | Many effect ferment fertilizer that a kind of agricultural wastes make | |
| CN101200387B (en) | Preparation of microorganium fertilizer by using bacilluslaterosporus and bacillus subtilis | |
| CN101317625B (en) | Comprehensive treatment method for pollution of livestock and poultry aquaculture, special feedstuff bridging agent, biological edible warm pad and biological organic fertilizer | |
| CN103360151A (en) | Biological organic composite fertilizer containing humic acid chelate | |
| CN104926530A (en) | High-efficiency biological organic fertilizer for organic crops and preparation method thereof | |
| CN108409380A (en) | A kind of method that agriculture wastes prepare environment-friendly fertilizer | |
| CN103342615A (en) | Phosphorus and potassium decomposing biological organic fertilizer and preparation method thereof | |
| CN101987799A (en) | Artificial turf as well as manufacturing method and use method thereof | |
| CN102775207A (en) | Chicken manure/pig manure mixed fermentation fertilizer and preparation method thereof | |
| CN102898189A (en) | Method for producing functional biological organic base fertilizer by using poultry excrement | |
| CN102696458A (en) | Edible fungus compost, production method thereof and edible fungus culture process | |
| CN103130555A (en) | Biological organic foliar fertilizer and preparation method thereof | |
| CN106946630A (en) | A kind of organic fungi-manure using sludge as raw material and preparation method thereof | |
| CN108863658A (en) | A kind of charcoal base organic fertilizer and preparation method | |
| CN108046948A (en) | A kind of special sheep manure fermentation fertilizer of leguminous forage and preparation method thereof | |
| CN104387135A (en) | Method for producing functional biological organic base fertilizer by using chicken manure | |
| CN102352316A (en) | Composite germ pulp, and production method and application thereof | |
| CN107129327A (en) | Microbial manure and preparation method thereof | |
| CN104761321A (en) | Ecological total-effect organic fertilizer and preparation method thereof | |
| CN107396888A (en) | A kind of method of ecologically raising pigs | |
| CN108264405A (en) | A kind of organic fertilizer prepared using cassava trade waste and preparation method thereof | |
| CN104926535A (en) | Spirulina peptide bio-organic fertilizer | |
| CN106966832A (en) | A kind of ecological organic fertilier and preparation method thereof | |
| CN108264404A (en) | A kind of organic fertilizer prepared using alcohol waste mash and preparation method thereof | |
| CN105565918A (en) | Plant nutrition package green organic fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150708 |
|
| RJ01 | Rejection of invention patent application after publication |