CN104688796B - Method based on ultrasonic wave extraction maca bioactive ingredients - Google Patents
Method based on ultrasonic wave extraction maca bioactive ingredients Download PDFInfo
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Abstract
The present invention discloses a kind of method based on ultrasonic wave extraction maca bioactive ingredients, and maca root tuber is handled using high pressure wall breaking technology and ultrasonic wave extraction, final to obtain the product containing high concentration maca bioactive ingredients.The extracting method of the present invention can effectively extract the bioactive ingredients such as maca alkaloid in maca root tuber, maca ene, macamide, improve raw material availability and reduce production cost relative to existing extractive technique.The product prepared simultaneously according to the inventive method, has higher bioactivity, health-care effect is substantially better than prior art.
Description
Technical field
The present invention relates to the extracting method of maca bioactive ingredients, and in particular to one kind is given birth to based on ultrasonic wave extraction maca
The method of thing active component.
Background technology
Maca (Maca) also known as Peruvian ginseng, maka etc., it is that South America is annual or biennial herbaceous plant, category
Cruciferae (Cruciferae) separate row Vegetable spp (Lepidium L), specific kind is also disputable, in the world it is believed that
Lepidium two kinds of meyenii Walp and Lepidium peruvianum Chacon growth and breeding, Phytochemistry and
Almost there is no difference on health-care efficacy, therefore be collectively referred to as " Maca " (transliteration is " maca ").Maca is in South America andes region
The cultivation history for having had thousands of years is one of indispensable crop.With good health-care effect.
Maca alkaloid can improve the quantity of the quantity of animal graaffian follicle corpusculum, the mobility of sperm and sperm, so as to
It is remarkably improved the fertility of mammal and fish.And the alkaloid in maca can act on hypothalamus and brain hangs down
Body, there is the regulation incretory such as function such as adrenal gland, thyroid gland, pancreas, ovary, so as to balance hormone, thus can use and give
Treat female climacteric syndrome.
Common alkaloid extracting method has sour water direct extraction method, infusion process, oozes rumble method, hot cocurrent flow method, ultrasonic extraction
Deng.At present, only it is related to the ultrasonic extraction of maca alkaloid.Therefore, maca, maca are handled using the plant supercritical ultrasonics technology of routine
The yield of alkaloid is relatively low, causes existing maca alkaloid cost high and health-care effect unobvious.
The content of the invention
In view of this, the present invention, which disclose one kind, can greatly improve maca alkaloid yield, reduction Maca reactive into mitogenetic
Produce cost, strengthen the extraction process of maca bioactive ingredients physiological function.
The purpose of the present invention is achieved through the following technical solutions：
Based on the method for ultrasonic wave extraction maca bioactive ingredients, including following process：
S1. pre-treatment maca root tuber；
S2. it is particle the maca root tuber to be crushed；
S3. broken wall treatment is carried out to the particle, obtains broken wall product；
S4. using ultrasonic extraction processing broken wall product；Filtrate is collected by filtration, obtains the first filtrate；
S5. filter residue is collected, and using purifying 90~150min of flooding, filtrate is collected by filtration, obtains the second filtrate；
S6. first filtrate and the second filtrate are mixed, maca bioactive ingredients product is obtained through Aging storage.
Pre-treatment alleged by S1 includes the conventional pre-treatment step of prior art, such as cleans, dries.Alleged by the present invention
Maca root tuber is water content in 50wt%~75wt% commercially available prod.S2 can use any prior art to realize, such as existing
Beater grinder.It is little particle that the root tuber of raw material, which is crushed, is advantageous to increase the specific surface area of raw material, at follow-up broken wall
In reason, there is bigger area, which to bear the rise of pressure and moment, reduces, so as to improve broken wall efficiency.Original through broken wall treatment
Material, maca alkaloid, macamide, the activated protein yield of ultrasonic wave extraction are far above the ultrasonic wave extraction of prior art.And
Filter residue after ultrasonic wave extraction is further extracted with hydro-thermal method, can thoroughly be extracted under the premise of lower-cost residual in filter residue
Remaining active component, the utilization rate of raw material is improved, waste can be reduced in large-scale production and application and effectively controls maca to live
The production cost of property composition.Aging storage can be standing, lucifuge preservation 20~30 days.Broken wall treatment can also use any plantation
Thing wall breaking technology is realized.
Further, the broken wall treatment refers to the particle being placed in the container of sealing, is passed through and vessel volume phase
Deng ammonia, and heat container to 60~65 DEG C of container Nei Wenduda and maintain 3~5min；Stop ammonia, and to defeated in container
Enter gauge pressure in pressure-air to container to be 0.5~0.7Mpa and maintain 60~130 seconds, by the gauge pressure in container in 0.5~1 second
It is reduced to 0pa；Volume is used as the sodium citrate of vessel volume 40%~80% and alcohol mixture eluant container, collecting washings is
For the broken wall product；
The particle diameter of the particle is 0.2~0.5mm；
Further, use ultrasonic extraction processing broken wall product refer to by the broken wall product and quality for its 2~5
Ethanol mixing again, it is heated to using 80KHz 30~50min of ultrasonic wave extraction after 30~40 DEG C, filter residue is carried out after filtering
Extract again three times；The ethanol that the multiple extraction refers to use quality to be 3~5 times of filter residue is at 50~80 DEG C with 60KHz ultrasound
Ripple extracts 90~140min；Merging filtrate obtains first filtrate.
The ultrasonic wave of prior art is less efficient to Maca reactive constituents extraction, is primarily due to the thin of maca root tuber cell
Cell wall mechanical strength is higher, and the mechanical crushing broken wall efficiency of in general cutting type is low, causes substantial amounts of cell inclusion by cell
Wall and obstruct.The present invention applies pressure-air to particle first, and in abrupt release pressure, makes individual cells instantaneous expansion and make
Cell membrane is burst, and cellular content largely ejects.Prior art has use to be filled with ammonia makes raw material low at a higher temperature
Compression swelling and realize shell-broken effect, but for maca raw material, high temperature also may be used there is a possibility that active material therein deforms
The substance escapings such as wherein volatile maca ene, mustard oil can be made, be lost in.After tested, it is passed through ammonia and is favorably improved cell membrane
Unstability, the expansion being allowed to bursts that process is more violent, and additionally aiding reduces maca ene, the volatilization efficiency of mustard oil, carries
The yield of high maca ene and mustard oil etc..Sodium citrate can ensure the stability that broken wall product is subjected to after ammonia corrosion, especially
It is that reactive protein therein can be avoided to inactivate.Sodium citrate-the alcohol mixture is 2~3 parts by weight of lemon
The mixture of sour sodium and 60~90 parts of ethanol.Grain（Half）Footpath is that 0.2~0.5mm particle is easy to process by prior art,
And and can makes the cell membrane of more many cells expose in atmosphere.The container can select the steam-explosion jar of prior art.The present invention
In ultrasonic wave extraction any ultrasonic wave extraction equipment can be selected realize, can also use common ultrasonic extraction to realize.
Extracted using repeated ultrasonic ripple, the yield of Maca reactive composition especially maca alkaloid can be obviously improved.Due to different
Activity component concentration in extraction time material is different, and different extraction conditions, Neng Gou are set through the multiple authentication present invention
Highest yield is obtained under minimum cost, while product has highest bioactivity.Particularly, the present invention is by container
High pressure is defined to 0.5~0.7Mpa of gauge pressure.Upon discharge, volatile maca ene is by along with the pressure of release for too high pressure
Power is lost in, and too low pressure can not thorough fracturing cell walls.But the cell membrane in the present invention, handled through ammonia is easy to brokenly
It is broken, so as to be minimized the pressure in container, prevent the loss of volatile substances.The input quantity of ammonia is preferably vessel volume
1.3~2.5 times.
Further, the Aging storage in the S6 is directed to add 1~6 portion of reed in 50~70 parts by weight of filtrate
Luxuriant growth root system suspension, while 2~8 parts of vitamin Cs, 10~17 parts of glucose, 2~15 parts of ethanol are added, with 35 in sealing container
~45 DEG C of anoxybiotics obtain maca bioactive ingredients product after handling 5~21.The biology that after-ripening step can improve product is living
Property, improve the maca aromatic odor in product.The secretion of aloe root system and microorganism contribute to the progress of after-ripening step.
Further, the aloe root system suspension is 10~19 parts by weight of Chinese aloe root, is crushed as 1~3mm's
In the bead that segment is immersed in 20~40 parts of sterilized waters and 2~15 parts of particle diameters are 1mm, using rotary shaker 200~
The filtrate of acquisition is filtered after being vibrated 6 minutes under 250 turns/min speed.
Chinese aloe root is cut into segment and extruded using bead, contributes to the release of above-mentioned secretion.
The Chinese aloe root is the fresh aloe root of prior art, and its weight is fresh weight.
Particularly preferred, the process S1 pre-treatments maca root tuber refers to crisp to maca root tuber 5 low temperature cell membranes of progress
Chemical industry sequence；The low temperature cell membrane embrittlement process is specially that the temperature of maca root tuber is down into -9~-5 DEG C in 60s, is kept
The mass fraction containing 30~40 DEG C of constant temperature is immersed into after 30min as 60min in 0.01% sodium chloride solution, every kilogram
Maca root tuber sprays the acetic acid solution that 3~6g mass fractions are 6%.
Under cryogenic, intracellular Free water will condense into trickle ice crystal and puncture cell membrane and cell membrane.And
Mobility declines, is brittle plant cell membrane without materials such as cholesterol at low temperature, is easily broken.Especially in acetic acid and chlorination
After sodium processing, the cell membrane of maca is also and its easily damaged in a low temperature of repeatedly, cell wall answering for slight crack dress is occurred
Power bears a little, to be advantageous to the thorough of cell membrane in follow-up broken wall process and crush.
Embodiment
For the ease of it will be appreciated by those skilled in the art that being described in further detail below in conjunction with embodiment to the present invention：
Embodiment 1
The present embodiment provides a kind of method based on ultrasonic wave extraction maca bioactive ingredients, including following process：
S1. pre-treatment maca root tuber；
S2. it is particle above-mentioned maca root tuber to be crushed；
S3. broken wall treatment is carried out to above-mentioned particle, obtains broken wall product；
S4. using ultrasonic extraction processing broken wall product；Filtrate is collected by filtration, obtains the first filtrate；
S5. filter residue is collected, and using purifying flooding 125min, filtrate is collected by filtration, obtains the second filtrate；
S6. above-mentioned first filtrate and the second filtrate are mixed, maca bioactive ingredients product is obtained through Aging storage.
Above-mentioned broken wall treatment refers to above-mentioned particle being placed in the container of sealing, is passed through ammonia, and heats container to container
60 DEG C of Nei Wenduda simultaneously maintains 4min；Stop input ammonia, and be to inputting gauge pressure in pressure-air to container in container
0.6Mpa is simultaneously maintained 90 seconds, and the gauge pressure in container is reduced into 0pa in 0.5 second；Using alcohol flushing container, washings are collected
As above-mentioned broken wall product；
The particle diameter of above-mentioned particle is 0.4mm；
Ultrasonic extraction processing broken wall product is used to refer to quality mix above-mentioned broken wall product for its 3 times ethanol
Close, be heated to using 80KHz ultrasonic wave extraction 45min after 35 DEG C, filter residue is extracted again three times after filtering；It is above-mentioned to carry again
Take the ethanol for referring to use quality to be 4 times of filter residue at 60 DEG C with 60KHz ultrasonic wave extraction 100min；On merging filtrate obtains
State the first filtrate.
Aging storage in above-mentioned S6 is directed to add 3 parts of aloe root system suspensions in 55 parts by weight of filtrate, simultaneously
7 parts of vitamin Cs, 12 parts of glucose, 9 parts of ethanol are added, maca life is obtained after being handled 14 with 40 DEG C of anoxybiotics in sealing container
Thing active component product.
Above-mentioned aloe root system suspension is 17 parts by weight of Chinese aloe root, the segment crushed as 3mm be immersed in 30 parts it is sterile
Water and 8 parts of particle diameters are in 1mm bead, are filtered after being vibrated 6 minutes under 250 turns/min speed using rotary shaker
The filtrate of acquisition.Above-mentioned operation S1 pre-treatment maca root tubers refer to carry out 5 low temperature cell membrane embrittlement processes to maca root tuber；On
It is specially that the temperature of maca root tuber is down into -9~-5 DEG C in 60s to state low temperature cell membrane embrittlement process, is kept it after 30min
It is immersed in 60min in the sodium chloride solution that the mass fraction containing 30~40 DEG C of constant temperature is 0.01%, every kilogram of maca root tuber sprinkling
3~6g mass fractions are 6% acetic acid solution.
In the present embodiment, 1 part is 1kg.Using 60 parts of maca root tuber（Water content 70wt%）, it is concentrated finally to prepare
73.25kg product, wherein maca alkaloid is 327g after measured, and maca ene content is 109g, and macamide is
0.999g, polysaccharide 538.39g, glucosinolate 981g.
Embodiment 2
The present embodiment provides a kind of method based on ultrasonic wave extraction maca bioactive ingredients, including following process：
S1. pre-treatment maca root tuber；
S2. it is particle above-mentioned maca root tuber to be crushed；
S3. broken wall treatment is carried out to above-mentioned particle, obtains broken wall product；
S4. using ultrasonic extraction processing broken wall product；Filtrate is collected by filtration, obtains the first filtrate；
S5. filter residue is collected, and using purifying flooding 150min, filtrate is collected by filtration, obtains the second filtrate；
S6. above-mentioned first filtrate and the second filtrate are mixed, maca bioactive ingredients product is obtained through Aging storage.
Above-mentioned broken wall treatment refers to above-mentioned particle being placed in the container of sealing, is passed through ammonia, and heats container to container
60 DEG C of Nei Wenduda simultaneously maintains 5min；Stop input ammonia, and be to inputting gauge pressure in pressure-air to container in container
0.5Mpa is simultaneously maintained 130 seconds, and the gauge pressure in container is reduced into 0pa in 0.5 second；Using alcohol flushing container, collect and rinse
Thing is above-mentioned broken wall product；
The particle diameter of above-mentioned particle is 0.5mm；
Ultrasonic extraction processing broken wall product is used to refer to quality mix above-mentioned broken wall product for its 2 times ethanol
Close, be heated to using 80KHz ultrasonic wave extraction 30min after 40 DEG C, filter residue is extracted again three times after filtering；It is above-mentioned to carry again
Take the ethanol for referring to use quality to be 5 times of filter residue at 50 DEG C with 60KHz ultrasonic wave extraction 140min；On merging filtrate obtains
State the first filtrate.
Aging storage in above-mentioned S6 is directed to add 6 parts of aloe root system suspensions in 50 parts by weight of filtrate, simultaneously
2 parts of vitamin Cs, 17 parts of glucose, 2 parts of ethanol are added, maca biology is obtained after being handled 5 with 45 DEG C of anoxybiotics in sealing container
Active component product.
Above-mentioned aloe root system suspension is 19 parts by weight of Chinese aloe root, the segment crushed as 1mm be immersed in 40 parts it is sterile
Water and 2 parts of particle diameters are in 1mm bead, are filtered after being vibrated 6 minutes under 250 turns/min speed using rotary shaker
The filtrate of acquisition.
In the present embodiment, 1 part is 1kg.Using 60 parts of maca root tuber（Water content 70wt%）, it is concentrated finally to prepare
75.69kg product, after measured wherein maca alkaloid（Total alkali, acetic acid titration）Content is 277.20g, and maca ene content is
98.11g, macamide 1.237g, polysaccharide 529.82g, glucosinolate 997.83g.
Embodiment 3
The present embodiment provides a kind of method based on ultrasonic wave extraction maca bioactive ingredients, including following process：
S1. pre-treatment maca root tuber；
S2. it is particle above-mentioned maca root tuber to be crushed；
S3. broken wall treatment is carried out to above-mentioned particle, obtains broken wall product；
S4. using ultrasonic extraction processing broken wall product；Filtrate is collected by filtration, obtains the first filtrate；
S5. filter residue is collected, and using purifying flooding 90min, filtrate is collected by filtration, obtains the second filtrate；
S6. above-mentioned first filtrate and the second filtrate are mixed, maca bioactive ingredients product is obtained through Aging storage.
Above-mentioned broken wall treatment refers to above-mentioned particle being placed in the container of sealing, is passed through ammonia, and heats container to container
65 DEG C of Nei Wenduda simultaneously maintains 3min；Stop input ammonia, and be to inputting gauge pressure in pressure-air to container in container
0.7Mpa is simultaneously maintained 60 seconds, and the gauge pressure in container is reduced into 0pa in 1 second；Using alcohol flushing container, collecting washings is
For above-mentioned broken wall product；
The particle diameter of above-mentioned particle is 0.2mm；
Ultrasonic extraction processing broken wall product is used to refer to quality mix above-mentioned broken wall product for its 5 times ethanol
Close, be heated to using 80KHz ultrasonic wave extraction 50min after 30 DEG C, filter residue is extracted again three times after filtering；It is above-mentioned to carry again
Take the ethanol for referring to use quality to be 3 times of filter residue at 80 DEG C with 60KHz ultrasonic wave extraction 90min；On merging filtrate obtains
State the first filtrate.
Aging storage in above-mentioned S6 is directed to add 1 part of aloe root system suspension in 70 parts by weight of filtrate, simultaneously
8 parts of vitamin Cs, 10 parts of glucose, 15 parts of ethanol are added, maca life is obtained after being handled 21 with 35 DEG C of anoxybiotics in sealing container
Thing active component product.
Above-mentioned aloe root system suspension is 10 parts by weight of Chinese aloe root, the segment crushed as 3mm be immersed in 20 parts it is sterile
Water and 15 parts of particle diameters are mistake after being vibrated 6 minutes under 200 turns/min speed using rotary shaker in 1mm bead
Filter the filtrate obtained.
In the present embodiment, 1 part is 1kg.Using 60 parts of maca root tuber（Water content 70wt%）, it is concentrated finally to prepare
74.75kg product, wherein maca alkaloid is 309g after measured, and maca ene content is 87g, and macamide is
0.952g, polysaccharide 511.47g, glucosinolate 961g.
Embodiment 4
The present embodiment provides a kind of method based on ultrasonic wave extraction maca bioactive ingredients, including following process：
S1. pre-treatment maca root tuber；
S2. it is particle above-mentioned maca root tuber to be crushed；
S3. broken wall treatment is carried out to above-mentioned particle, obtains broken wall product；
S4. using ultrasonic extraction processing broken wall product；Filtrate is collected by filtration, obtains the first filtrate；
S5. filter residue is collected, and using purifying flooding 125min, filtrate is collected by filtration, obtains the second filtrate；
S6. above-mentioned first filtrate and the second filtrate are mixed, maca bioactive ingredients product is obtained through Aging storage.
Above-mentioned pre-treatment is that clean, dry to water content conventional to the progress of fresh maca root tuber is 70wt%.Above-mentioned broken wall
Processing refers to above-mentioned particle being placed in the container of sealing, is passed through ammonia, and heat container to 60 DEG C of container Nei Wenduda and tie up
Hold 4min；Stop input ammonia, and be 0.6Mpa and maintain 90 seconds to inputting gauge pressure in pressure-air to container in container,
The gauge pressure in container is reduced to 0pa in 0.5 second；Using alcohol flushing container, it is above-mentioned broken wall product to collect washings；
The particle diameter of above-mentioned particle is 0.4mm；
Ultrasonic extraction processing broken wall product is used to refer to quality mix above-mentioned broken wall product for its 3 times ethanol
Close, be heated to using 80KHz ultrasonic wave extraction 45min after 35 DEG C, filter residue is extracted again three times after filtering；It is above-mentioned to carry again
Take the ethanol for referring to use quality to be 4 times of filter residue at 60 DEG C with 60KHz ultrasonic wave extraction 100min；On merging filtrate obtains
State the first filtrate.
In the present embodiment, 1 part is 1kg.Using 60 parts of maca root tuber（Water content 70wt%）, it is concentrated finally to prepare
73.25kg product, wherein maca alkaloid is 265g after measured, and maca ene content is 65g, and macamide is
0.907g, polysaccharide 458.39g, glucosinolate 710g.
Embodiment 5
The present embodiment provides a kind of method based on ultrasonic wave extraction maca bioactive ingredients, including following process：
S1. pre-treatment maca root tuber；
S2. it is particle above-mentioned maca root tuber to be crushed；
S3. broken wall treatment is carried out to above-mentioned particle, obtains broken wall product；
S4. using ultrasonic extraction processing broken wall product；Filtrate is collected by filtration, obtains the first filtrate；
S5. filter residue is collected, and using purifying flooding 125min, filtrate is collected by filtration, obtains the second filtrate；
S6. above-mentioned first filtrate and the second filtrate are mixed, maca bioactive ingredients product is obtained through Aging storage.
Above-mentioned broken wall treatment crushes for pressure-air.
Aging storage in above-mentioned S6 is directed to add 3 parts of aloe root system suspensions in 55 parts by weight of filtrate, simultaneously
7 parts of vitamin Cs, 12 parts of glucose, 9 parts of ethanol are added, maca life is obtained after being handled 14 with 40 DEG C of anoxybiotics in sealing container
Thing active component product.
Above-mentioned aloe root system suspension is 17 parts by weight of Chinese aloe root, the segment crushed as 3mm be immersed in 30 parts it is sterile
Water and 8 parts of particle diameters are in 1mm bead, are filtered after being vibrated 6 minutes under 250 turns/min speed using rotary shaker
The filtrate of acquisition.
By adult male mice（113.11 ± 5.72g of body weight）It is divided into 6 groups, every group 50, every group of difference gavage feeding is real
Example 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 and physiological saline are applied, feeding dosage is 0.5g.Feeding process continues
50 days.Mouse feeding food and water since the childhood.
30 mouse of every group of selection, its swimming is driven in being gone in off the deep end, mouse blood sugar concentration is tested after 120min.And
Test the growth rate of mouse weight in 50 days.Its result is as shown in table 1.
The mouse test results of table 1
It is the wherein specific implementation of the present invention above, its description is more specific and detailed, but can not therefore manage
Solve as the limitation to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, do not departing from
On the premise of present inventive concept, various modifications and improvements can be made, these obvious alternative forms belong to this hair
Bright protection domain.
Claims (4)
1. a kind of method based on ultrasonic wave extraction maca bioactive ingredients, including following process：S1. pre-treatment maca
Root tuber；S2. it is particle the maca root tuber to be crushed；S3. broken wall treatment is carried out to the particle, obtains broken wall product
；S4. using ultrasonic extraction processing broken wall product；Filtrate is collected by filtration, obtains the first filtrate；S5. filter residue is collected,
And using purifying 90~150min of flooding, filtrate is collected by filtration, obtains the second filtrate；S6. first filter is mixed
Liquid and the second filtrate, maca bioactive ingredients product is obtained through Aging storage；The broken wall treatment refers to put the particle
In the container of sealing, ammonia is passed through, and heats container to 60~65 DEG C of container Nei Wenduda and maintains 3~5min
；Stop input ammonia, and be 0.5~0.7Mpa and maintenance 60 to inputting gauge pressure in pressure-air to container in container
~130 seconds, the gauge pressure in container is reduced to 0pa in 0.5~1 second；Using alcohol flushing container, collect and rinse
Thing is the broken wall product；The particle diameter of the particle is 0.2~0.5mm；Broken wall is handled using ultrasonic extraction
Product refers to that by the broken wall product be that its 2~5 times ethanol mixes with quality, is used after being heated to 30~40 DEG C
80KHz 30~50min of ultrasonic wave extraction, filter residue is extracted again three times after filtering；The multiple extraction refers to adopt
With the ethanol that quality is 3~5 times of filter residue at 50~80 DEG C with 60KHz ultrasonic wave extraction 90~
140min ；Merging filtrate obtains first filtrate.
2. the method according to claim 1, it is characterised in that：Aging storage in the S6 is directed to by weight
1~6 part of aloe root system suspension of addition in the filtrate of 50~70 parts of meter, while 2~8 parts of vitamin Cs of addition,
10~17 parts of glucose, 2~15 parts of ethanol, 5~21 are handled with 35~45 DEG C of anoxybiotics in sealing container
Maca bioactive ingredients product is obtained in the future.
3. according to the method for claim 2, it is characterised in that：The aloe root system suspension is by weight 10~19
The Chinese aloe root of part, the segment crushed as 1~3mm are immersed in 20~40 parts of sterilized waters and 2~15 parts of particle diameters
For in 1mm bead, after being vibrated 6 minutes under 200~250 turns/min speed using rotary shaker
Filter the filtrate obtained.
4. the method according to claim 1, it is characterised in that：The process S1 pre-treatments maca root tuber refers to pair
Maca root tuber carries out 5 low temperature cell membrane embrittlement processes；The low temperature cell membrane embrittlement process is specially by maca root tuber
Temperature be down to -9~-5 DEG C in 60s, be immersed into the matter containing 30~40 DEG C of constant temperature after keeping 30min
60min in the sodium chloride solution that fraction is 0.01% is measured, it is 6% that every kilogram of maca root tuber, which sprays 3~6g mass fractions,
Acetic acid solution.
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| CN105146511A (en) * | 2015-07-31 | 2015-12-16 | 昆明博尚生物技术有限公司 | Processing method for improving specific active components of Peruvian ginseng |
| CN105941819A (en) * | 2016-04-27 | 2016-09-21 | 云南还原医学生物科技有限公司 | Extraction method of maca crude proteins |
| CN106723001B (en) * | 2017-01-13 | 2021-01-22 | 中国科学院过程工程研究所 | Method for increasing content of amide in maca, prepared product and application of product |
| CN107982439B (en) * | 2017-12-22 | 2020-09-29 | 营养屋(成都)生物医药有限公司 | Dendrobium officinale and American ginseng capsule, preparation method thereof and extraction method of dendrobium officinale |
| CN108685969A (en) * | 2018-06-11 | 2018-10-23 | 南方医科大学 | A kind of extracting method of maca total alkaloid |
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