CN105941819A - Extraction method of maca crude proteins - Google Patents

Extraction method of maca crude proteins Download PDF

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Publication number
CN105941819A
CN105941819A CN201610270797.6A CN201610270797A CN105941819A CN 105941819 A CN105941819 A CN 105941819A CN 201610270797 A CN201610270797 A CN 201610270797A CN 105941819 A CN105941819 A CN 105941819A
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CN
China
Prior art keywords
meyenii walp
lepidinm meyenii
crude protein
extraction
extracting method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610270797.6A
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Chinese (zh)
Inventor
叶承虎
罗丽莉
倪红标
杜军
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YUNNAN REDUCTION MEDICAL BIOLOGICAL TECHNOLOGY Co Ltd
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YUNNAN REDUCTION MEDICAL BIOLOGICAL TECHNOLOGY Co Ltd
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Priority to CN201610270797.6A priority Critical patent/CN105941819A/en
Publication of CN105941819A publication Critical patent/CN105941819A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/006Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a processing method of maca and particularly relates to an extraction method of maca crude proteins. The extraction method comprises the following steps: ultrafine grinding, alkali dissolving, ultrasonic extracting, centrifugal separating and filtering. In order to overcome the disadvantages that a traditional alkali dissolving and acid precipitating method is long in extraction time and relatively high in extraction temperature, seriously destroys proteins, and is large in energy consumption, the ultrasonic extraction technology is introduced in the method, and the ultrasonic wave is applied to the medium, so that strong tensile stress appears in an extract to form a negative pressure, which forces the raw material cell walls to be ruptured instantly, accelerates dissolution and diffusion efficiencies of the intracellular proteins in the extract, and thereby can effectively shorten the extraction time and increase the extraction rate. The method has the advantages of being simple in operation, easy to control, low in cost, good in extraction effect, large-scale in production, etc. By using the method, the maca crude protein extraction rate is 10%-14%.

Description

A kind of extracting method of Lepidinm meyenii Walp crude protein
Technical field
The processing method that the present invention relates to Lepidinm meyenii Walp, the extracting method of a kind of Lepidinm meyenii Walp crude protein.
Background technology
Lepidinm meyenii Walp is Cruciferae separate row Vegetable spp annual herb plant, originates in Peru more than height above sea level 3500m Mountain area, Andean, can be without fertilizer, anoxia, day and night temperature greatly, normally give birth under the most ice-bound unique environments Long." Maca " is that the English of plant Lepidium meyenii is commonly called as, and Lepidinm meyenii Walp is in nutritional labeling and increasing simultaneously Strong energy, effect aspect of endurance can match in excellence or beauty with Radix Ginseng, are commonly referred to as the most again " PeruvianGinseng (Peru's Radix Ginseng) ".
Fresh Lepidinm meyenii Walp (MACA) moisture content 10.4%, protein 10.2%, fat 2.2%, carbohydrate 59%, fiber 8.5%, ash 4.9%.Containing the aminoacid of abundant needed by human, such as: arginine (9.94%), Aspartic acid (9.17%), glutamic acid (15.6%) and lysine (54.5%) etc..Lepidinm meyenii Walp (MACA) Fatty acid in the content of unsaturated fatty acid account for 52.7% (its Linoleic acid 18.5%, linolenic acid 8.87%), Containing glucosinolate (glucosinolates) sterol and potassium, calcium, ferrum etc..Lepidinm meyenii Walp (MACA) lipid portion Position, containing Lepidinm meyenii Walp alkene (maceaene) and macamide (mecamide), also includes N-benzyl caprylamide, N- Benzyl-16-hydroxyl-9-oxidation-10E, 12E, 14E-pungent certain herbaceous plants with big flowers three acrylamide, N-benzyl-16 hydroxyl-9,16- Dioxy-10E, 12E, 14E-pungent certain herbaceous plants with big flowers three acrylamide and other 17 Lepidinm meyenii Walp alkene and the homologue of macamide. Lepidinm meyenii Walp alkene and macamide be considered as in Lepidinm meyenii Walp (MACA) extract tool promote sexual function active substance it One.
In presently disclosed document material, also do not have a kind of to extract the method for crude protein in Lepidinm meyenii Walp, invent one Fine maca deep processing is very important by the extracting method planting Lepidinm meyenii Walp crude protein.
Summary of the invention
The problem that it is an object of the invention to exist for above-mentioned technology, it is provided that the extraction side of a kind of Lepidinm meyenii Walp crude protein Method.
For achieving the above object, the technical solution used in the present invention is: the extracting method of a kind of Lepidinm meyenii Walp crude protein, Step includes:
(1) superfine grinding: Lepidinm meyenii Walp raw material dries after cleaning, and carries out superfine grinding after section, prepares pueraria root powder;
(2) alkali is molten: the alkaline solution adding its weight 10~50 times of weight in pueraria root powder extracts, and utilizes This alkaline solution regulation pH of mixed maintains 7.5~11.5;
(3) supersound extraction: mixed liquor adds supersound extraction tank and extracts 20~100min;
(4) centrifugation: remix liquid after supersound extraction and be centrifuged separating 15~20min;
(5) filter: filter after centrifugation, collect filtrate, add salt acid for adjusting pH extremely neutrality, After static 15min, recentrifuge separates, and curdled milk is Lepidinm meyenii Walp crude protein.
Further, in step (1), the fineness of described pueraria root powder is 10~20 mesh.
Further, in step (2), described alkaline solution is NaOH, and concentration is 1mol/L.
Further, in step (3), described supersound extraction power is 260W~660W, described supersound extraction Temperature 50~70 degree.
Further, in step (4), described centrifugation centrifuge speed is 3400r/min.
Further, in step (5), described centrifugation centrifuge speed is 3400r/min.
Further, in step (5), the mass concentration adding hydrochloric acid is 1mol/L.
The present invention also provides for a kind of Lepidinm meyenii Walp crude protein prepared by said method.
The present invention also provides for the application in food of a kind of Lepidinm meyenii Walp crude protein prepared by method, the thick egg of preparation Can be used for athlete or the egg albumen powder of body-building food in vain.
The ultimate principle of the present invention is to use alkali extraction-acid precipitation, utilizes most protein isoelectric point, IP at acid model Enclosing, when protein is in alkaline environment, most protein can be dissolved out from cell, works as lysate When regulation pH value is to isoelectric points of proteins, due to the destruction of outer layer charge, can gather between protein molecule Close precipitation, thus separate out from solution.
The method have the benefit that: in the present invention, for overcoming tradition alkali extraction-acid precipitation extraction time The defects such as length, Extracting temperature is higher, serious to protein breakdown, energy resource consumption is big, introduce ultrasound wave and carry Taking technique, by ul-trasonic irradiation in medium so that extracting solution is internal occurs that powerful tension is formed negative Pressure, forces raw material cell wall moment to rupture, and accelerates intracellular protein dissolving in extracting solution and diffusion effect Rate, thus can effectively shorten extraction time, improve extraction efficiency;The inventive method has simple to operate, Easily controllable, with low cost, extraction effect is preferable, and the feature such as can be mass-produced, and utilizes side of the present invention Method, the extraction ratio of Lepidinm meyenii Walp crude protein is 10%~14%.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Ground describes, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole realities Execute example.Based on the embodiment in the present invention, those of ordinary skill in the art are not before making creative work Put the every other embodiment obtained, broadly fall into the scope of protection of the invention.
Embodiment 1
A kind of extracting method of Lepidinm meyenii Walp crude protein, step includes:
(1) superfine grinding: selected Lepidinm meyenii Walp raw material 100kg dries after cleaning, and carries out superfine grinding after section, Prepared fineness is the pueraria root powder of 10 mesh;
(2) alkali is molten: adds NaOH solution 1000kg that concentration is 1mol/L in pueraria root powder and dissolves, and profit 7.5 are maintained by this alkaline solution regulation pH of mixed;
(3) supersound extraction: mixed liquor adds supersound extraction tank and extracts 20min, and supersound extraction power is 660W, Extracting temperature 70 degree;
(4) centrifugation: remixing liquid after supersound extraction and be centrifuged separating 15min, centrifuge speed is 3400r/min;
(5) filter: filter after centrifugation, collect filtrate, add salt acid for adjusting pH extremely neutrality, After static 15min, recentrifuge separates, and centrifuge speed is 3400r/min, and curdled milk is Lepidinm meyenii Walp crude protein 12kg。
Embodiment 2
A kind of extracting method of Lepidinm meyenii Walp crude protein, step includes:
(1) superfine grinding: Lepidinm meyenii Walp raw material 50kg dries after cleaning, and carries out superfine grinding after section, prepares Fineness is the pueraria root powder of 20 mesh;
(2) alkali is molten: adds NaOH solution 1200kg that concentration is 1mol/L in pueraria root powder and dissolves, And utilize this alkaline solution regulation pH of mixed to maintain 9.5;
(3) supersound extraction: mixed liquor adds supersound extraction tank and extracts 60min, and supersound extraction power is 460W, Extracting temperature 60 degree;
(4) centrifugation: remixing liquid after supersound extraction and be centrifuged separating 17min, centrifuge speed is 3400r/min;
(5) filter: filter after centrifugation, collect filtrate, add salt acid for adjusting pH extremely neutrality, After static 15min, recentrifuge separates, and centrifuge speed is 3400r/min, and curdled milk is Lepidinm meyenii Walp albumen 6.5kg。
Embodiment 3
A kind of extracting method of Lepidinm meyenii Walp crude protein, step includes:
(1) superfine grinding: Lepidinm meyenii Walp raw material 200kg dries after cleaning, and carries out superfine grinding after section, prepares Fineness is the pueraria root powder of 20 mesh;
(2) alkali is molten: adds NaOH solution 10000kg that concentration is 1mol/L in pueraria root powder and dissolves, And utilize this alkaline solution regulation pH of mixed to maintain 11.5;
(3) supersound extraction: mixed liquor adds supersound extraction tank and extracts 100min, and extraction power is 660W, Temperature 70 degree;
(4) centrifugation: remixing liquid after supersound extraction and be centrifuged separating 20min, centrifuge speed is 3400r/min;
(5) filter: filter after centrifugation, collect filtrate, add salt acid for adjusting pH extremely neutrality, After static 15min, recentrifuge separates, and centrifuge speed is 3400r/min, and curdled milk is Lepidinm meyenii Walp protein 25 kg.
Those skilled in the art of the present technique are appreciated that unless otherwise defined, all terms used herein (bag Include technical term and scientific terminology) have and being commonly understood by of the those of ordinary skill in art of the present invention Identical meaning.Should also be understood that those terms defined in such as general dictionary should be understood that There is the meaning consistent with the meaning in the context of prior art, and unless defined as here, no Meeting idealization or the most formal implication are explained.
One of ordinary skill in the art will appreciate that: the parts in device in embodiment can be according to embodiment Describe in the device being distributed in embodiment, it is also possible to carry out respective change and be disposed other than one of the present embodiment Or in multiple device.The parts of above-described embodiment can merge into parts, it is also possible to is further split into Multiple subassemblies.
It should be noted last that: above example only in order to illustrative not limiting technical scheme, Although being described in detail the present invention with reference to above-described embodiment, those of ordinary skill in the art should manage Solve: still the present invention can be modified or equivalent, without departing from the spirit and scope of the present invention Any modification or partial replacement, it all should be contained in the middle of scope of the presently claimed invention.

Claims (9)

1. the extracting method of a Lepidinm meyenii Walp crude protein, it is characterised in that step includes:
(1) superfine grinding: Lepidinm meyenii Walp raw material dries after cleaning, and carries out superfine grinding after section, prepares pueraria root powder;
(2) alkali is molten: the alkaline solution adding its weight 10~50 times of weight in pueraria root powder extracts, and utilizes This alkaline solution regulation pH of mixed maintains 7.5~11.5;
(3) supersound extraction: mixed liquor adds supersound extraction tank and extracts 20~100min;
(4) centrifugation: remix liquid after supersound extraction and be centrifuged separating 15~20min;
(5) filter: filter after centrifugation, collect filtrate, add salt acid for adjusting pH extremely neutrality, After static 15min, recentrifuge separates, and curdled milk is Lepidinm meyenii Walp crude protein.
The extracting method of Lepidinm meyenii Walp crude protein the most according to claim 1, it is characterised in that step (1) In, the fineness of described pueraria root powder is 10~20 mesh.
The extracting method of Lepidinm meyenii Walp crude protein the most according to claim 1, it is characterised in that step (2) In, described alkaline solution is NaOH, and concentration is 1mol/L.
The extracting method of Lepidinm meyenii Walp crude protein the most according to claim 1, it is characterised in that step (3) In, described supersound extraction power is 260W~660W, the temperature 50 of described supersound extraction~70 degree.
The extracting method of Lepidinm meyenii Walp crude protein the most according to claim 1, it is characterised in that step (4) In, described centrifugation centrifuge speed is 3400r/min.
The extracting method of Lepidinm meyenii Walp crude protein the most according to claim 1, it is characterised in that step (5) In, described centrifugation centrifuge speed is 3400r/min.
The extracting method of Lepidinm meyenii Walp crude protein the most according to claim 1, it is characterised in that step (5) In, the mass concentration adding hydrochloric acid is 1mol/L.
8. the Lepidinm meyenii Walp crude protein prepared by method described in any one of claim 1-7.
The Lepidinm meyenii Walp crude protein that the most according to claim 8, prepared by method application in food.
CN201610270797.6A 2016-04-27 2016-04-27 Extraction method of maca crude proteins Pending CN105941819A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103275012A (en) * 2013-06-08 2013-09-04 中国科学院过程工程研究所 Preparation method and application of Maca alkaloid
CN103918965A (en) * 2013-01-15 2014-07-16 北京同仁堂健康药业股份有限公司 Physical fatigue alleviating beverage
CN104688796A (en) * 2015-02-04 2015-06-10 广东长兴生物科技股份有限公司 Method for extracting maca bioactive components based on ultrasonic wave
CN105192723A (en) * 2015-07-01 2015-12-30 中国科学院过程工程研究所 Maca dietary fiber as well as preparation method and application thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103918965A (en) * 2013-01-15 2014-07-16 北京同仁堂健康药业股份有限公司 Physical fatigue alleviating beverage
CN103275012A (en) * 2013-06-08 2013-09-04 中国科学院过程工程研究所 Preparation method and application of Maca alkaloid
CN104688796A (en) * 2015-02-04 2015-06-10 广东长兴生物科技股份有限公司 Method for extracting maca bioactive components based on ultrasonic wave
CN105192723A (en) * 2015-07-01 2015-12-30 中国科学院过程工程研究所 Maca dietary fiber as well as preparation method and application thereof

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Application publication date: 20160921