CN105994946A - Preparation method of high calcium lactate fermented feed - Google Patents
Preparation method of high calcium lactate fermented feed Download PDFInfo
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Abstract
The present invention relates to the technical fields of feeds, enzyme preparations and microbial fermentation, and particularly relates to a preparation method of a high calcium lactate fermented feed. One or more of soybean meal, food processing by-products, brans and molasses syrup are mixed with clean water, starch materials and stone powder evenly, lactic acid bacterium liquid and compound enzymes are added, the mixture is subjected to a natural enzymolysis, the enzymolyzed mixture is subjected to an anaerobic fermentation, and the anaerobic fermented mixture is prepared into a fermentation culture medium wet base. The stone powder is used repeatedly to adjust pH, and then the lactic acid bacteria are inoculated into the mixture to conduct fermentation repeatedly to prepare a high calcium lactate fermented feed wet base. The high calcium lactate fermented feed is rich in calcium lactate, lactic acids, small peptides, amino acids, vitamins and probiotics, has the characteristics of being high in nutrition, easy in digestion, good in palatability, etc., and can significantly increase the lactation amount of sows and the feed intake, and reduce the piglet diarrhea.
Description
Technical field
The invention belongs to feedstuff, enzyme preparation and technical field of microbial fermentation, be specifically related to one
The preparation method of high calcium lactate zymolysis feedstuff.
Background technology
China is populous nation, is also that the first in the world is raised pigs big country.2015, China's herding
The industry output value reaches 3.5 trillion yuan, and live pig delivers 6.43 hundred million for sale, wherein sow livestock on hand 45,000,000,
Carnis Sus domestica yield 54,870,000 tons.According to Ministry of Agriculture's statistical data, within 2015, national commercial feed is produced
Amount reaches 2.18 hundred million tons.Wherein, complete feed 1.79 hundred million tons, concentrated feed 21,510,000 tons,
Premix material 6,400,000 tons, including pig feed yield 89,740,000 tons, fowl material yield 90,000,000 tons, water
Produce 3,500,000 tons of the feedstuffs such as 53,000,000 tons of feedstuff, 8,500,000 tons of cattle and sheep feedstuff, rabbit Nyctereutes procyonoides house pet,
Whole nation sow feed more than 500 ten thousand tons.Expect the year two thousand twenty whole nation mixed feed yield to be up to
More than 2.2 hundred million tons, wherein sow feed is up to more than more than 800 ten thousand tons.
It is known that the difficult point of the exploitation of feed product, development is the feed for piglet, how
Improve the feed for piglet palatability, digestibility and reduction suckling piglet diarrhoea and become animal battalion in recent years
The problem that foster scholar actively inquires into.Suckling piglet metabolism is vigorous, and relative growth is rapid, to day
The requirement of grain nutrient substance is high, but its gastral function is unsound, gastral weight and appearance
Amassing less and send out the region between the heart and the diaphragm not exclusively, gastric acid secretion is not enough, and gastrointestinal micro-flora is variable, digests
It is unsound that the region between the heart and the diaphragm is sent out by enzyme system, digestive enzyme hyposecretion.Suckling piglet protein digestibility is bad, protein
Easily produce some spoilage product, cadaverine, putrescine, histamine etc. at hindgut, stimulate intestinal mucosa, damage
Evil intestinal villi film, causes intestinal contents osmotic pressure to raise, and intestinal dehydration causes diarrhoea.
System's acid power (Acid-binding capacity), also known as daily ration system acid power, is that daily ration combines
The ability of acid, i.e. refers to the acidity buffer capacity that acidic materials are had by the daily ration of certain mass, also
It is referred to as daily ration acid buffering power, acid counteragent, acid adhesion or acid attachment coefficient, usually makes 1
Kg feedstuff pH value is down to the milliequivalent of required hydrochloric acid when 3.0.Research shows: feedstuff
System's acid power is the lowest, and intestine of young pigs pH is the lowest；Otherwise, the highest.Therefore, in order to reduce son
The diarrhoea of pig, people start to seek to be acid power feedstuff on the low side, in the hope of reducing piglet
Intestinal pH.
Stone powder main component is exactly calcium carbonate.Its wide material sources, but it is acid, and power is the highest.One
For as, after piglet diet addition is more than 0.8%, just it is easier to cause piglet diarrhea.
And calcium lactate is a kind of organic calcium, it is that acid power is on the low side, is one of optimal calcium source nutrition of piglet.
Therefore, people begin to use calcium lactate to replace stone powder.
In recent years, fermented feed, as green, environment-friendly feed, becomes at livestock product safety
Under this overall background of the subject matter of animal husbandry development, have become as current Animal nutrition research
Focus.Feed fermentation can not only open up feed resource, improves feedstuff palatability, but also can
To improve feed digestibility.It is worth mentioning that fermented feedstuff is in suppression is digested and assimilated
The breeding of escherichia coli, Salmonella and some pathogenic bacterias and pathogen is imitated with growth aspect
Fruit substantially, therefore, the most increasingly paid attention to by countries in the world in recent years, feed fermentation by fermented feed
Technology is in the ascendant.But, fermented feed lactic acid content typically ratio is relatively low, wet basis state next
As only up to 2-3%, generally not over 5%.Trace it to its cause is that lactic acid can feedback suppression breast
Acid bacterium self fermentation and acid.Therefore, the acid bacteria fermented effect of solid state fermentation conditions stimulating milk secretion how is improved
Rate is also a new problem.
How fermented feed is combined with calcium lactate and be one and highly study and inquire into
New problem.But, there is not yet report or the technology of high calcium lactate fermented feed.
Summary of the invention
, piglet diarrhea rate height, feed fermentation high for current pig starter feed stone powder system of China acid power
The difficult problem that lactic acid content is on the low side, it is an object of the invention to: provide a kind of rich in calcium lactate, breast
Peptide sour, little, aminoacid, vitamin and probiotic bacteria, have that nutrition is high, easy to digest, palatability
The feature such as good, can significantly improve milk yield of sow, hence it is evident that increase feed intake, significantly reduce piglet
The high calcium lactate zymolysis feedstuff of diarrhoea.
To achieve these goals, the technical solution adopted in the present invention is:
A kind of preparation method of high calcium lactate zymolysis feedstuff, step is as follows:
1., the preparation of one time fermentation culture medium wet basis:
By one or more in bean cake, food processing side-product, furfur, molasses syrup with dry
After water purification, starch material, stone powder mix homogeneously, after high temperature sterilize, cooling, prepare solid fermentation
Culture medium, then accesses lactobacillus inoculation liquid and compound enzyme, after anaerobic fermentation, prepares one time fermentation
Culture medium wet basis；
2., the preparation of ferment in second time culture medium wet basis:
After one time fermentation culture medium wet basis and stone powder being sufficiently mixed uniformly, access lactobacillus inoculation
Liquid, after anaerobic fermentation, prepares ferment in second time culture medium wet basis；
3., the preparation of three fermentation medium wet basis:
After ferment in second time culture medium wet basis and stone powder being sufficiently mixed uniformly, access lactobacillus inoculation
Liquid, after anaerobic fermentation, prepares three fermentation medium wet basis；
4., the preparation of high calcium lactate zymolysis feedstuff:
After three fermentation medium wet basis and stone powder being sufficiently mixed uniformly, access lactobacillus inoculation
Liquid, after anaerobic fermentation, prepares high calcium lactate zymolysis feedstuff wet basis or dries prepared high calcium lactate ferment
Solve feedstuff butt.
Further, starch material during described starch material is National agricultural portion feedstuff catalogue
One or more, for Semen Maydis, Oryza glutinosa, crack rice, Semen Tritici aestivi, flour, Herba bromi japonici, Fructus Hordei Vulgaris, height
In fine strain of millet, Radix Ipomoeae, Maninot esculenta crantz., Rhizoma Dioscoreae esculentae, Rhizoma Solani tuber osi, or the dilated product of above-mentioned starch material one
Plant or multiple；
Described molasses syrup is in National agricultural portion feedstuff catalogue the one of sugar molasse syrup
Kind or multiple, for sucrose slurry/powder, glucose syrup/powder, fructose syrup/powder, maltodextrin slurry/powder,
High fructose syrup/powder, maltose syrup/powder, oligosaccharide syrup/powder, Sugar cane molasses, beet molasses, big
One or more in bean molasses；
Described food processing side-product is feedstuff in National agricultural portion feedstuff catalogue
One or more, for cottonseed meal, rapeseed meal, Semen arachidis hypogaeae dregs, wheat gluten flour, Zein powder,
Rice protein powder, DDGS, wheat bran, Testa oryzae, acid-sludge, Chinese liquor distiller grains, medicated beer distiller grains, shallow lake
One or more in pulverized sugar slag.
Preferably, described starch material is Semen Maydis, crack rice, Semen Tritici aestivi, flour, or above-mentioned shallow lake
One or more in the dilated product of amylaceous substance；
Described molasses syrup be the one in glucose syrup/powder, Sugar cane molasses, beet molasses or
Multiple；
Described food processing side-product be cottonseed meal, rapeseed meal, Semen arachidis hypogaeae dregs, Zein powder,
One or more in DDGS, wheat bran, Testa oryzae, acid-sludge, medicated beer distiller grains, starch sugar slag.
Further, the preparation condition of step 1. middle one time fermentation culture medium wet basis is as follows:
Clean water, starch material, bean cake, food processing side-product, furfur, molasses syrup
Weight ratio is 100:1～1000:0～500:0～300:0～200:0～100；
Lactobacillus inoculation liquid inoculum concentration accounts for the mass percent of solid-state fermentation culture medium: 0.5%～
50%；
Compound enzyme addition accounts for the mass percent of solid-state fermentation culture medium: 0.0002%～
2%；
Fermentation temperature: 5～50 DEG C；
Starting fermentation pH: natural；
Fermented type: anaerobic fermentation；
Moisture content in medium: 15%～90%；
Fermentation time: 3～480 hours；
Compound enzyme by following enzyme live ratio enzyme form: protease, cellulase, xylanase,
The enzyme of pectase and mannase live than for 100:1～600:1～600:1～600:1～
200。
Preferably, the preparation condition of step 1. middle one time fermentation culture medium wet basis is as follows:
Clean water, starch material, bean cake, food processing side-product, furfur, molasses syrup
Weight ratio is 100:60～80:20～30:10～20:5～10:1～5；
Lactobacillus inoculation liquid inoculum concentration accounts for the mass percent of solid-state fermentation culture medium: 4%～
8%；
Compound enzyme addition accounts for the mass percent of solid-state fermentation culture medium: 0.10%～
0.50%；
Fermentation temperature: 25～26 DEG C；
Starting fermentation pH: natural；
Fermented type: anaerobic fermentation；
Moisture content in medium: 45%～55%；
Fermentation time: 60～84 hours；
Compound enzyme by following enzyme live ratio enzyme form: protease, cellulase, xylanase,
The enzyme of pectase and mannase live than for 100:30～60:30～60:30～60:10～
20。
Further, described step 2., 3., 4. in access lactobacillus inoculation liquid anaerobic fermentation conditions
As follows:
One time fermentation culture medium wet basis or ferment in second time culture medium wet basis or three fermentation medium
Wet basis is 100:0.01～10 with the weight ratio of stone powder；
PH regulator is extremely: 5～9；
Lactobacillus inoculation liquid inoculum concentration accounts for one time fermentation culture medium wet basis or ferment in second time culture medium is wet
The mass percent of base or three fermentation medium wet basis is: 0.1%～20%；
Fermentation temperature: 4～40 DEG C；
Fermented type: anaerobic fermentation；
Fermentation time: 2～240 hours.
Preferably, described step 2., 3., 4. in access lactobacillus inoculation liquid anaerobic fermentation conditions such as
Under:
One time fermentation culture medium wet basis or ferment in second time culture medium wet basis or three fermentation medium
Wet basis is 100:0.5～1 with the weight ratio of stone powder；
PH regulator is extremely: 6～7；
Lactobacillus inoculation liquid inoculum concentration accounts for one time fermentation culture medium wet basis or ferment in second time culture medium is wet
The mass percent of base or three fermentation medium wet basis is: 1%～2%；
Fermentation temperature: 24～26 DEG C；
Fermented type: anaerobic fermentation；
The beneficial effects of the present invention is: zymolysis feedstuff prepared by the present invention is rich in calcium lactate, breast
Peptide sour, little, aminoacid, vitamin and probiotic bacteria, have that nutrition is high, easy to digest, palatability
The feature such as good, can significantly improve milk yield of sow, hence it is evident that increase feed intake, significantly reduce piglet
Diarrhoea.
Detailed description of the invention
Below with reference to embodiment, the present invention is described in detail.
Embodiment 1
1., the preparation of one time fermentation culture medium wet basis:
80kg clean water, 60kg Semen Maydis, 15kg are cracked rice, 30kg bean cake, 10kg wheat bran,
After 5kg Sugar cane molasses and the mixing of 1kg stone powder, after high temperature sterilize, cooling, prepare solid fermentation training
Support base, access lactobacillus inoculation liquid 15kg and be mixed into 80g compound enzyme, under the conditions of 22-28 DEG C,
Carry out anaerobic fermentation 60 hours, prepare 210-212kg one time fermentation culture medium wet basis.
Wherein, compound enzyme is made up of the enzyme of following enzyme ratio alive compound enzyme: protease, cellulose
The enzyme of enzyme, xylanase, pectase and mannase lives ratio for 100:50:40:45:
15。
2., the preparation of ferment in second time culture medium wet basis:
After 210-212kg one time fermentation culture medium wet basis and 3kg stone powder being mixed, access 20kg
Lactobacillus inoculation liquid, regulates pH to 6-8, under the conditions of 25-26 DEG C, carries out anaerobic fermentation 48
Hour, prepare 228-232kg ferment in second time culture medium wet basis.
3., the preparation of three fermentation medium wet basis:
After 228-232kg ferment in second time culture medium wet basis and 3kg stone powder being mixed, access 20kg
Lactobacillus inoculation liquid, regulates pH to 6-8, under the conditions of 25-26 DEG C, carries out anaerobic fermentation 48
Hour, prepare tri-fermentation medium wet basis of 248-252kg.
4., the preparation of high calcium lactate zymolysis feedstuff:
After tri-fermentation medium wet basis of 248-252kg and 5kg stone powder being mixed, access 30kg
Lactobacillus inoculation liquid, regulates pH to 6-8, under the conditions of 25-26 DEG C, carries out anaerobic fermentation 48
Hour, prepare 275-278kg height calcium lactate zymolysis feedstuff wet basis, or dry prepared 100-105kg
High calcium lactate zymolysis feedstuff butt.
Embodiment 2
1., the preparation of one time fermentation culture medium wet basis:
50kg clean water, 40kg Semen Maydis, 30kg are cracked rice, 20kg Semen Tritici aestivi, 25kg bean cake,
After 10kg wheat bran, 5kg beet molasses and the mixing of 1kg stone powder, prepare after high temperature sterilize, cooling
Solid-state fermentation culture medium, accesses lactobacillus inoculation liquid 20kg and is mixed into 100g compound enzyme,
Under the conditions of 22-28 DEG C, carry out anaerobic fermentation 60 hours, prepare the training of 200-205kg one time fermentation
Support base wet basis.
Wherein, compound enzyme is made up of the enzyme of following enzyme ratio alive: protease, cellulase, wood are poly-
The enzyme of carbohydrase, pectase and mannase lives ratio for 100:35:55:45:20.
2., the preparation of ferment in second time culture medium wet basis:
After 200-205kg one time fermentation culture medium wet basis and 4kg stone powder being mixed, access 25kg
Lactobacillus inoculation liquid, regulates pH to 6-8, under the conditions of 25-26 DEG C, carries out anaerobic fermentation 48
Hour, prepare 225-230kg ferment in second time culture medium wet basis.
3., the preparation of three fermentation medium wet basis:
After 225-230kg ferment in second time culture medium wet basis and 5kg stone powder being mixed, access 25kg
Lactobacillus inoculation liquid, regulates pH to 6-8, under the conditions of 25-26 DEG C, carries out anaerobic fermentation 48
Hour, prepare tri-fermentation medium wet basis of 238-242kg.
4., the preparation of high calcium lactate zymolysis feedstuff:
After tri-fermentation medium wet basis of 238-242kg and 6kg stone powder being mixed, access 30kg
Lactobacillus inoculation liquid, regulates pH to 6-8, under the conditions of 25-26 DEG C, carries out anaerobic fermentation 48
Hour, prepare 262-265kg height calcium lactate zymolysis feedstuff wet basis, or dry prepared
118-122kg height calcium lactate zymolysis feedstuff butt.
One, Major Nutrient material content in the high calcium lactate zymolysis feedstuff of embodiment 1 preparation, is shown in
Table 1.
The high calcium lactate zymolysis feed product Middle nutrition content of material of table 1, embodiment 1 preparation
Two, the high calcium lactate zymolysis feedstuff of embodiment 1 preparation feeds ablactational baby pig effect.
For inquiring into the high calcium lactate zymolysis feedstuff of embodiment 1 preparation, ablactational baby pig is fed effect
Really, the DLY three way cross piglet 120 of selective body heavy phase nearly (body weight 7.5 ± 0.50kg),
Being randomly divided into test group and matched group, test group 60, matched group 60, each group is all provided with 6
Individual repetition, each repetition 10.
Test group is identical with matched group piglet basal diet trophic level, is powder.Matched group
Not using high calcium lactate zymolysis feedstuff, test group uses interpolation quality hundred in basal diet respectively
Mark is the high calcium lactate zymolysis feedstuff of 4%, 8% and 12%.Basal diet configures with G state
Mark GB/T5915 piglet, grow-finish swine feed standard are reference.Result of the test is as follows:
By table 2 result it can be seen that basal diet adds 4%, 8% and 12% high lactic acid
After calcium zymolysis feedstuff, the daily gain of three way cross piglet respectively than matched group improve 9.71%,
7.30%, 9.62%, feed intake improves 4.45%, 6.57%, 3.62% than matched group respectively,
Feedstuff-meat ratio reduces by 3.42%, 6.85%, 6.85% than matched group respectively.
The impact on Production Performance of Weaning Pigs of the high calcium lactate zymolysis feedstuff of table 2, embodiment 1 preparation
Result shows: high calcium lactate zymolysis feedstuff can improve the daily gain of ablactational baby pig, improves
Feed intake, strengthens premunition, hence it is evident that improve feed efficiency.
Three, the high calcium lactate zymolysis feedstuff of embodiment 1 preparation feeds grower pigs effect.
For inquiring into the high calcium lactate zymolysis feedstuff feeding effect to grower pigs of embodiment 1 preparation,
Choose age in days, parity, DLY tri-crossbreeding 160 that body weight is close, be randomly divided into two
Group, respectively test group and matched group.The test phase of raising in advance is 7 days, is just trying the phase 29 days.Examination
Test group identical with matched group piglet basal diet trophic level.Matched group does not use high calcium lactate ferment
Solving feedstuff, test group uses adds the high calcium lactate that mass percent is 8% in basal diet
Zymolysis feedstuff.Result of the test is as follows:
The impact on grower pigs production performance of the high calcium lactate zymolysis feedstuff of table 3, embodiment 1 preparation
| Project | Matched group | Test group |
| Head number (head) | 80 | 80 |
| Beginning phase counterpoise (kg) | 23.08 | 24.04 |
| Terminate counterpoise (kg) | 38.74 | 40.28 |
| Head all weightening finishes (kg/ head) | 15.66 | 16.24 |
| Average daily gain (kg/ head/day) | 0.54 | 0.56 |
| Averagely search for food heavily (kg/ head/day) | 1.28 | 1.22 |
| Efficiency of feed utilization | 2.37 | 2.18 |
| Sickness rate (%) | 6.25 | 1.25 |
By table 3 result it can be seen that matched group daily gain is 540 grams, test group is 560 grams.
After basal diet adds 8% high calcium lactate zymolysis feedstuff, grower pigs daily gain improves 3.7%.
Matched group efficiency of feed utilization is 2.37:1, and test group is 2.18:1.Basal diet adds
After 8% high calcium lactate zymolysis feedstuff, grower pigs efficiency of feed utilization improves 8.0%.
The average daily ingestion amount of matched group is 1.28 kilograms, and test group is 1.22 kilograms.Basis day
After grain adds 8% high calcium lactate zymolysis feedstuff, daily ingestion amount improves 4.7%.
The sickness rate of test group is 1.25%, and matched group is 6.25%, and it is high that basal diet adds 8%
After calcium lactate zymolysis feedstuff, sickness rate reduces 5.0%.
Above content is only citing made for the present invention and explanation, affiliated the art
Described specific embodiment is made various amendment or supplements or use class by technical staff
As mode substitute, without departing from the design of invention or surmount the claims and defined
Scope, protection scope of the present invention all should be belonged to.
Claims (7)
1. the preparation method of one kind high calcium lactate zymolysis feedstuff, it is characterised in that step is as follows:
1., the preparation of one time fermentation culture medium wet basis:
By one or more in bean cake, food processing side-product, furfur, molasses syrup with dry
After water purification, starch material, stone powder mix homogeneously, after high temperature sterilize, cooling, prepare solid fermentation
Culture medium, then accesses lactobacillus inoculation liquid and compound enzyme, after anaerobic fermentation, prepares one time fermentation
Culture medium wet basis；
2., the preparation of ferment in second time culture medium wet basis:
After one time fermentation culture medium wet basis and stone powder being sufficiently mixed uniformly, access lactobacillus inoculation
Liquid, after anaerobic fermentation, prepares ferment in second time culture medium wet basis；
3., the preparation of three fermentation medium wet basis:
After ferment in second time culture medium wet basis and stone powder being sufficiently mixed uniformly, access lactobacillus inoculation
Liquid, after anaerobic fermentation, prepares three fermentation medium wet basis；
4., the preparation of high calcium lactate zymolysis feedstuff:
After three fermentation medium wet basis and stone powder being sufficiently mixed uniformly, access lactobacillus inoculation
Liquid, after anaerobic fermentation, prepares high calcium lactate zymolysis feedstuff wet basis or dries prepared high calcium lactate ferment
Solve feedstuff butt.
The preparation method of a kind of high calcium lactate zymolysis feedstuff the most according to claim 1,
It is characterized in that:
Described starch material be starch material in National agricultural portion feedstuff catalogue one or
Multiple, for Semen Maydis, Oryza glutinosa, crack rice, Semen Tritici aestivi, flour, Herba bromi japonici, Fructus Hordei Vulgaris, Sorghum vulgare Pers., Radix Ipomoeae,
One or more in Maninot esculenta crantz., Rhizoma Dioscoreae esculentae, Rhizoma Solani tuber osi, or the dilated product of above-mentioned starch material；
Described molasses syrup is in National agricultural portion feedstuff catalogue the one of sugar molasse syrup
Kind or multiple, for sucrose slurry/powder, glucose syrup/powder, fructose syrup/powder, maltodextrin slurry/powder,
High fructose syrup/powder, maltose syrup/powder, oligosaccharide syrup/powder, Sugar cane molasses, beet molasses, big
One or more in bean molasses；
Described food processing side-product is feedstuff in National agricultural portion feedstuff catalogue
One or more, for cottonseed meal, rapeseed meal, Semen arachidis hypogaeae dregs, wheat gluten flour, Zein powder,
Rice protein powder, DDGS, wheat bran, Testa oryzae, acid-sludge, Chinese liquor distiller grains, medicated beer distiller grains, shallow lake
One or more in pulverized sugar slag.
The preparation side of a kind of high calcium lactate zymolysis feedstuff the most according to claim 1 and 2
Method, it is characterised in that:
Described starch material is Semen Maydis, crack rice, Semen Tritici aestivi, flour, or above-mentioned starch material
One or more in dilated product；
Described molasses syrup be the one in glucose syrup/powder, Sugar cane molasses, beet molasses or
Multiple；
Described food processing side-product be cottonseed meal, rapeseed meal, Semen arachidis hypogaeae dregs, Zein powder,
One or more in DDGS, wheat bran, Testa oryzae, acid-sludge, medicated beer distiller grains, starch sugar slag.
The preparation method of a kind of high calcium lactate zymolysis feedstuff the most according to claim 1,
It is characterized in that, the preparation condition of step 1. middle one time fermentation culture medium wet basis is as follows:
Clean water, starch material, bean cake, food processing side-product, furfur, molasses syrup
Weight ratio is 100:1～1000:0～500:0～300:0～200:0～100；
Lactobacillus inoculation liquid inoculum concentration accounts for the mass percent of solid-state fermentation culture medium: 0.5%～
50%；
Compound enzyme addition accounts for the mass percent of solid-state fermentation culture medium: 0.0002%～
2%；
Fermentation temperature: 5～50 DEG C；
Starting fermentation pH: natural；
Fermented type: anaerobic fermentation；
Moisture content in medium: 15%～90%；
Fermentation time: 3～480 hours；
Compound enzyme by following enzyme live ratio enzyme form: protease, cellulase, xylanase,
The enzyme of pectase and mannase live than for 100:1～600:1～600:1～600:1～
200。
The preparation method of a kind of high calcium lactate zymolysis feedstuff the most according to claim 4,
It is characterized in that, the preparation condition of step 1. middle one time fermentation culture medium wet basis is as follows:
Clean water, starch material, bean cake, food processing side-product, furfur, molasses syrup
Weight ratio is 100:60～80:20～30:10～20:5～10:1～5；
Lactobacillus inoculation liquid inoculum concentration accounts for the mass percent of solid-state fermentation culture medium: 4%～
8%；
Compound enzyme addition accounts for the mass percent of solid-state fermentation culture medium: 0.10%～
0.50%；
Fermentation temperature: 25～26 DEG C；
Starting fermentation pH: natural；
Fermented type: anaerobic fermentation；
Moisture content in medium: 45%～55%；
Fermentation time: 60～84 hours；
Compound enzyme by following enzyme live ratio enzyme form: protease, cellulase, xylanase,
The enzyme of pectase and mannase live than for 100:30～60:30～60:30～60:10～
20。
The preparation method of a kind of high calcium lactate zymolysis feedstuff the most according to claim 1,
It is characterized in that, described step 2., 3., 4. in access lactobacillus inoculation liquid anaerobic fermentation conditions such as
Under:
One time fermentation culture medium wet basis or ferment in second time culture medium wet basis or three fermentation medium
Wet basis is 100:0.01～10 with the weight ratio of stone powder；
PH regulator is extremely: 5～9；
Lactobacillus inoculation liquid inoculum concentration accounts for one time fermentation culture medium wet basis or ferment in second time culture medium is wet
The mass percent of base or three fermentation medium wet basis is: 0.1%～20%；
Fermentation temperature: 4～40 DEG C；
Fermented type: anaerobic fermentation；
Fermentation time: 2～240 hours.
The preparation method of a kind of high calcium lactate zymolysis feedstuff the most according to claim 6,
It is characterized in that, described step 2., 3., 4. in access lactobacillus inoculation liquid anaerobic fermentation conditions such as
Under:
One time fermentation culture medium wet basis or ferment in second time culture medium wet basis or three fermentation medium
Wet basis is 100:0.5～1 with the weight ratio of stone powder；
PH regulator is extremely: 6～7；
Lactobacillus inoculation liquid inoculum concentration accounts for one time fermentation culture medium wet basis or ferment in second time culture medium is wet
The mass percent of base or three fermentation medium wet basis is: 1%～2%；
Fermentation temperature: 24～26 DEG C；
Fermented type: anaerobic fermentation；
Fermentation time: 36～48 hours.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490304A (en) * | 2016-11-02 | 2017-03-15 | 徐亚洲 | Beneficial bacteria peptide high activity economic benefits and social benefits lactein and preparation method thereof |
| CN107897529A (en) * | 2017-11-24 | 2018-04-13 | 贵州大兴农业科技发展有限公司 | Low anti-feed of Tiny ecosystem and preparation method thereof |
| CN109717312A (en) * | 2019-03-07 | 2019-05-07 | 衢州丰帆科技有限公司 | Improve the pig feed additive and preparation method thereof of immune performance |
| CN111034854A (en) * | 2019-11-22 | 2020-04-21 | 合肥五粮泰生物科技有限公司 | Preparation method of palatable flavor enzymolysis fermentation wet feed for feed |
| CN112042808A (en) * | 2019-06-05 | 2020-12-08 | 周南 | Plant enzyme calcium and preparation method thereof |
| CN112515044A (en) * | 2020-12-01 | 2021-03-19 | 北京挑战农业科技有限公司 | Preparation method and application of fermentation premix capable of enhancing vitamin stability |
| TWI737276B (en) * | 2020-04-27 | 2021-08-21 | 東方學校財團法人東方設計大學 | Method for producing calcium lactate |
| US11856969B2 (en) | 2020-05-01 | 2024-01-02 | Packerland Whey Products Inc. | Compositions and methods for solidified fermented animal feed |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490304A (en) * | 2016-11-02 | 2017-03-15 | 徐亚洲 | Beneficial bacteria peptide high activity economic benefits and social benefits lactein and preparation method thereof |
| CN107897529A (en) * | 2017-11-24 | 2018-04-13 | 贵州大兴农业科技发展有限公司 | Low anti-feed of Tiny ecosystem and preparation method thereof |
| CN109717312A (en) * | 2019-03-07 | 2019-05-07 | 衢州丰帆科技有限公司 | Improve the pig feed additive and preparation method thereof of immune performance |
| CN112042808A (en) * | 2019-06-05 | 2020-12-08 | 周南 | Plant enzyme calcium and preparation method thereof |
| CN111034854A (en) * | 2019-11-22 | 2020-04-21 | 合肥五粮泰生物科技有限公司 | Preparation method of palatable flavor enzymolysis fermentation wet feed for feed |
| TWI737276B (en) * | 2020-04-27 | 2021-08-21 | 東方學校財團法人東方設計大學 | Method for producing calcium lactate |
| US11856969B2 (en) | 2020-05-01 | 2024-01-02 | Packerland Whey Products Inc. | Compositions and methods for solidified fermented animal feed |
| CN112515044A (en) * | 2020-12-01 | 2021-03-19 | 北京挑战农业科技有限公司 | Preparation method and application of fermentation premix capable of enhancing vitamin stability |
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