CN104649759B - The high carbon-based additive of soil that a kind of fermentation soybean stalk is prepared - Google Patents
The high carbon-based additive of soil that a kind of fermentation soybean stalk is prepared Download PDFInfo
- Publication number
- CN104649759B CN104649759B CN201510059562.8A CN201510059562A CN104649759B CN 104649759 B CN104649759 B CN 104649759B CN 201510059562 A CN201510059562 A CN 201510059562A CN 104649759 B CN104649759 B CN 104649759B
- Authority
- CN
- China
- Prior art keywords
- soil
- compound bacteria
- stalk
- rhodococcus
- high carbon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention belongs to soil conditioner technical field, specifically discloses the high carbon-based additive of soil that a kind of fermentation soybean stalk is prepared.Additive of the present invention is using soybean stalk as degraded raw material, and adds compound bacteria and is prepared, the compound bacteria includes each strain of following ratio：Phanerochaete chrysosporium：Koning trichoderma：Paracoccus denitrificans：Rhodococcus sp：Thread bacillus=1 ~ 3 of butanedioic acid:2~8:3~8:1~3:2~8.Nutritional ingredient of the invention according to soybean stalk, using five kinds of compound bacteria-fermenteds degraded soybean stalks, utilize complementarity and synergy multiple between strain, not only in a short time soybean stalk can be made sufficiently to be degraded, and the high carbon-based additive of the soil that is prepared can also improved soil physicochemical property, promote crop growth, induce crop enhancing resistance, strengthen cold-resistant, drought resisting, disease-resistant and anti-adversity ability, increase yield, improve product quality.
Description
Technical field
The present invention relates to soil conditioner technical field, in particular it relates to the soil that a kind of fermentation soybean stalk is prepared
The high carbon-based additive of earth.
Background technology
Stalk is the byproduct of crops, is remaining crop residues after seed harvest, including Cereal, beans, potato
Class, oil Class, crudefiber crop, and the various crop such as cotton, sugarcane, tobacco, melon and fruit.Stalk cellulose is as storage capacity in nature
Maximum renewable resource, the annual yield in the whole world are up to 100,000,000,000 t, and China is a large agricultural country, agricultural straw resource
Very abundant, at present only important crop material just have nearly 20 kinds, annual production is up to 7~1,000,000,000 t.With the tune of the structure of rural undertaking
Whole, the ratio of industrial crops stalk also increased.Crop material in addition to containing substantial amounts of carbon, also containing nitrogen, phosphorus, potassium, calcium,
Magnesium, sulphur, silicon and various trace elements, and rich in organic substances such as cellulose, hemicellulose, protein.If can fully have
Effect ground utilizes stalk cellulose, by as " grain, the energy, environment " three big crisis of face of mankind nowadays is alleviated, realizes agricultural
The effective way of sustainable development.
However, with the horizontal raising of life of farmers and the generally use of chemical fertilizer, a large amount of agricultural crop straws be incinerated or with
Ground is abandoned, and this not only wastes the resource of preciousness, but also brings various harm, such as：Environmental pollution, fire, soil desertification
Deng.The soil fertility decline of influence after to(for) the finer crop of those nutritional requirements is especially big, such as tobacco, and tobacco is to soil
The adaptability of earth is very strong, from light sandy soil to rich clay, from acid soil to alkaline soil, from light gray to yellow, red, black soil etc.
Various soil, the needs of tobacco growing development can be met.But the tobacco leaf flavor that different soils is produced is different,
Some peppery people that chokes of hardship, some aromatic alcohols and, what is had is greyish white bright, and some but stop working by grey black.So examined from the angle of quality of tobacco
Consider, requirement of the tobacco leaf production to edaphic condition is stricter.In order to improve the fertility of tobacco planting soil, tobacco grower tries flower
The stalks such as life, soybean, rape are added in soil after crushing, yet with the compact structure and complexity of stalk cellulose, certainly
Decomposed under the conditions of so slow so that these resources can not be utilized rapidly by agricultural land soil, so the addition of stalk not only can not
The fertility of increase soil in time, can influence the cultivation management of tobacco on the contrary.
Therefore, before stalk cellulose returning to the field, it need to be handled, and stalk is quickly handled using biotechnology is
The more solution method of current application.The biological treatment stalk method such as microorganism and enzyme, can effectively degrade its crude fibre, with increase
Soil fertility, improve soil granular structure, reach soil moisture conservation, breathe freely, be incubated, the effect that succession crop yield significantly improves, both subtracted
Lack the adverse effect of crop straw burning, also create good soil environment for the growth of succession crop.But current biology
Handle stalk method and the shortcomings that startup is slow, decomposition efficiency is low, fermentation time is long, specific aim difference be present, it is difficult to be widely popularized utilization.Cause
This, for different crop materials, seed selection and using crop material efficient degradation microbial strains, researches and develops high-efficiency degradation composite bacteria
Agent, it is one of direction of crop material comprehensive utilization of resources.
The content of the invention
In view of the shortcomings of the prior art, a kind of be prepared the invention provides fermentation soybean stalk soil is high carbon-based to be added
Add thing.The additive using Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, the thread bacillus of butanedioic acid it
Between cooperative effect, and by the reasonable compounding of five kinds of strains, can efficient degradation soybean stalk, while can effective improved soil
Physicochemical property, increase yield of tobacco and quality.
It is a further object of the present invention to provide the preparation method of above-mentioned additive.
It is a further object of the present invention to provide the application method of above-mentioned additive.
It is a further object of the present invention to provide application of the above-mentioned additive in bio-fertilizer is prepared.
The above-mentioned purpose of the present invention is achieved by the following technical programs.
The high carbon-based additive of soil that a kind of fermentation soybean stalk is prepared, the additive are using soybean stalk as drop
Raw material is solved, and adds compound bacteria and is prepared, the compound bacteria includes each strain of following ratio：Phanerochaete chrysosporium：Health
Family name's trichoderma：Paracoccus denitrificans：Rhodococcus sp：Thread bacillus=1~3 of butanedioic acid:2~8:3~8:1~3:2~8.
Cellulose, hemicellulose, and a small amount of lignin, protein, penta are mainly contained through researching and analysing, in soybean stalk
Glycan.According to the trophic structure of soybean stalk, inventor passes through substantial amounts of screening experiment, selects above-mentioned five kinds of bacterium to carry out rationally multiple
Match somebody with somebody：The extracellular protease of Paracoccus denitrificans secretion hydrolyzes the crude protein in soybean stalk；Phanerochaete chrysosporium can secrete
The lignin-degrading enzymes such as lignin peroxidase, manganese peroxidase, laccase, and hemicellulase；The yellow archespore hair
Flat lead fungi, Rhodococcus sp coordinate lignin degrading, play and break the barrier action that lignin and protein are formed, while make fiber
Element and hemicellulose are exposed, and then Phanerochaete chrysosporium continues to degrade to hemicellulose；The butanedioic acid is thread
Bacillus is one of rumen bacteria, and it can not only produce multifilament element enzyme body, including cellulase, inscribe hemicellulase and circumscribed
Hemicellulase, additionally it is possible to produce a large amount of cellulose binding proteins being positioned on cell outer surface, make the bacterium be adhered to stalk
On cellulose, therefore, the thread bacillus of butanedioic acid carries out efficient degradation to cellulose, hemicellulose；The koning trichoderma can be a large amount of
Exoglucanase, endoglucanase are synthesized, but the exoglucanase vigor produced is low, and the thread bacillus production of butanedioic acid
Exoglucanase vigor is high, therefore, can be to being exposed when koning trichoderma and the thread bacillus of butanedioic acid are cooperated
Cellulose, hemicellulose are more efficiently degraded；Meanwhile koning trichoderma can secrete zytase, wood can be effectively decomposed
Glycan and pentosan.In addition, during growth metabolism, Phanerochaete chrysosporium can increase the production of the humic acid such as humic acid
Amount, and promote the degraded of fats, polysaccharose substance；Koning trichoderma can also the material such as synthetic antibiotic, it is big to avoid degraded
The growth of other miscellaneous bacterias during beanstalk stalk；Paracoccus denitrificans can effectively adjust C/N ratios in soil additive, play improvement soil
The effect of earth structure；Rhodococcus sp can not only lignin degrading, additionally it is possible to polyhalo of degrading biphenyl, hydro carbons, coal and petroleum-type thing
Matter, play a part of soil environment reparation.The present invention is using the cooperative effect between above-mentioned five kinds of strains, obtained compound bacteria energy
Enough soybean stalks of more efficiently degrading, at the same can also effective improved soil physicochemical property, promote crop growth, induce
Crop strengthens resistance, enhancing cold-resistant, drought resisting, disease-resistant and anti-adversity ability, increases yield, improves product quality.
Preferably, the addition of the compound bacteria is the 0.8~4% of soybean stalk weight.
Preferably, the compound bacteria is made up of each strain of following ratio：Phanerochaete chrysosporium：Koning trichoderma：Denitrogenation
Secondary coccus：Rhodococcus sp：Thread bacillus=1~3 of butanedioic acid:4~8:5~8:2~3:4~8.
Preferably, the addition of the compound bacteria is the 1~2.5% of soybean stalk weight.
The preparation method of above-mentioned additive：Soybean stalk is cut to obtain degraded raw material, adds stalk weight 0.8 thereto
~4% compound bacteria, appropriate water is added, fermentation process turn over pushing away once after 2~3 days；Turned over again every 8~15 days and push away one
It is secondary, turn over push away twice altogether, obtain the additive；Wherein, the preparation of the compound bacteria comprises the following steps：
S1. Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, the thread bacillus of butanedioic acid are lived respectively
Change, cultivate into bacterium solution viable bacteria Particle density as 0.8~2 × 109Individual/gram；
S2. take the bacterium solution of above-mentioned five kinds of bacterium is well mixed to obtain composite bacteria liquid in proportion.
To control the water content of degeneration system, more conducively fermentative degradation, it is preferable that the addition of the water is stalk quality
30~40%.
Preferably, the preparation method of above-mentioned additive, each strain composition of the compound bacteria is Phanerochaete chrysosporium：Health
Family name's trichoderma：Paracoccus denitrificans：Rhodococcus sp：Thread bacillus=1~3 of butanedioic acid:4~8:5~8:2~3:When 4~8, fermentation process
Time is 2 days, turn over pushing away once；Turned over and push away once every 8~10 days again, turn over push away twice altogether, obtain the additive.Optimization
It can shorten to turn over during compatibility between strain and push away the time, improve degradation efficiency.
The application method of above-mentioned additive, it is characterised in that directly impose on field.
Application of the above-mentioned additive in bio-fertilizer is prepared.
Compared with prior art, the beneficial effects of the present invention are：
(1) nutritional ingredient of the invention according to soybean stalk, using compound bacteria-fermented degraded soybean stalk.Inventor has found
The thread bacillus of Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, butanedioic acid is applied to the degraded of soybean stalk
When, Phanerochaete chrysosporium, Rhodococcus sp coordinate lignin degrading, and the thread bacillus of Phanerochaete chrysosporium, butanedioic acid coordinates degraded
Hemicellulose, the thread bacillus of koning trichoderma, butanedioic acid coordinate degraded cellulose, have multiple complementarity between above-mentioned five kinds of bacterium
And synergy, not only in a short time soybean stalk can be made sufficiently to be degraded, and the soil high-carbon being prepared
Base additive can also improved soil physicochemical property, promote crop growth, induction crop enhancing resistance is enhancing cold-resistant, anti-
Non-irrigated, disease-resistant and anti-adversity ability, increase yield, improve product quality.
(2) the high carbon-based additive of soil of the present invention can avoid varied bacteria growing, be effectively increased agron and contain
Amount, C/N ratios are adjusted, rehabilitating soil, avoid miscellaneous bacteria from growing, increase soil granular structure, favourable crop grows, therefore energy
Improve the plantation yield of tobacco.
Embodiment
Present disclosure is further illustrated with reference to specific embodiment, but should not be construed as limiting the invention.
Without departing from the spirit and substance of the case in the present invention, the simple modifications or substitutions made to the inventive method, step or condition,
Belong to the scope of the present invention；Unless otherwise specified, technological means used in embodiment is well known to those skilled in the art
Conventional meanses.
The thread bacillus of the Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, butanedioic acid is common
It is commercially available.
Embodiment 1
A kind of high carbon-based additive of soil, is prepared by the following method to obtain：100kg soybean stalk is cut into 10~
30cm section shape adds the compound bacteria of stalk weight 0.8%, adds 30kg water, fermentation process 2 thereto as degraded raw material
My god, turn over pushing away once；Turned over and push away once every 8 days again, turn over push away twice altogether, obtain the high carbon-based additive of the soil.
Wherein, the preparation method of the compound bacteria is：
S1. Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, the thread bacillus of butanedioic acid are lived respectively
Change, cultivate into bacterium solution viable bacteria Particle density as 2 × 109Individual/gram；
S2. take respectively 114g Phanerochaete chrysosporiums bacterium solution, 190g koning trichodermas bacterium solution, 190g Paracoccus denitrificans bacterium solution,
The thread bacillus bacterium solution of 76g Rhodococcus sps bacterium solution, 229g butanedioic acids (i.e. proportionally 3:5:5:2:6) it is well mixed and obtains compound bacteria
Liquid.
Embodiment 2
A kind of high carbon-based additive of soil, is prepared by the following method to obtain：100kg soybean stalk is cleaved into 10
~30cm section shape adds the compound bacteria of stalk weight 1.2%, adds 35kg water, fermentation process 2 thereto as degraded raw material
My god, turn over pushing away once；Turned over and push away once every 10 days again, turn over push away twice altogether, obtain the high carbon-based additive of the soil.
Wherein, the preparation method of the compound bacteria is：
S1. Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, the thread bacillus of butanedioic acid are lived respectively
Change, cultivate into bacterium solution viable bacteria Particle density as 2 × 109Individual/gram；
S2. take respectively 109g Phanerochaete chrysosporiums bacterium solution, 218g koning trichodermas bacterium solution, 273g Paracoccus denitrificans bacterium solution,
The thread bacillus bacterium solution of 164g Rhodococcus sps bacterium solution, 436g butanedioic acids (i.e. proportionally 2:4:5:3:8) it is well mixed and obtains compound bacteria
Liquid.
Embodiment 3
A kind of high carbon-based additive of soil, is prepared by the following method to obtain：100kg soybean stalk is cut into 10~
Compound bacteria of the 30cm section shape as degraded raw material, thereto addition stalk weight 2%, addition 40kg water, fermentation process 2 days,
Turn over pushing away once；Turned over and push away once every 12 days again, turn over push away twice altogether, obtain the high carbon-based additive of the soil.
Wherein, the preparation method of the compound bacteria is：
S1. Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, the thread bacillus of butanedioic acid are lived respectively
Change, cultivate into bacterium solution viable bacteria Particle density as 109Individual/gram；
S2. take respectively 286g Phanerochaete chrysosporiums bacterium solution, 381g koning trichodermas bacterium solution, 571g Paracoccus denitrificans bacterium solution,
The thread bacillus bacterium solution of 190g Rhodococcus sps bacterium solution, 571g butanedioic acids (i.e. proportionally 3:4:6:2:6) it is well mixed and obtains compound bacteria
Liquid.
Embodiment 4
A kind of high carbon-based additive of soil, is prepared by the following method to obtain：100kg soybean stalk is cut into 10~
Compound bacteria of the 30cm section shape as degraded raw material, thereto addition stalk weight 3%, addition 40kg water, fermentation process 2 days,
Turn over pushing away once；Turned over and push away once every 15 days again, turn over push away twice altogether, obtain the high carbon-based additive of the soil.
Wherein, the preparation method of the compound bacteria is：
S1. Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, the thread bacillus of butanedioic acid are lived respectively
Change, cultivate into bacterium solution viable bacteria Particle density as 109Individual/gram；
S2. take respectively 563g Phanerochaete chrysosporiums bacterium solution, 563g koning trichodermas bacterium solution, 750g Paracoccus denitrificans bacterium solution,
The thread bacillus bacterium solution of 188g Rhodococcus sps bacterium solution, 938g butanedioic acids (i.e. proportionally 3:3:4:1:5) it is well mixed and obtains compound bacteria
Liquid.
Embodiment 5
A kind of high carbon-based additive of soil, is prepared by the following method to obtain：100kg soybean stalk is cut into 10~
Compound bacteria of the 30cm section shape as degraded raw material, thereto addition stalk weight 4%, addition 40kg water, fermentation process 3 days,
Turn over pushing away once；Turned over and push away once every 15 days again, turn over push away twice altogether, obtain the high carbon-based additive of the soil.
Wherein, the preparation method of the compound bacteria is：
S1. Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, the thread bacillus of butanedioic acid are lived respectively
Change, cultivate into bacterium solution viable bacteria Particle density as 0.8 × 109Individual/gram；
S2. take respectively 571g Phanerochaete chrysosporiums bacterium solution, 1524g koning trichodermas bacterium solution, 762g Paracoccus denitrificans bacterium solution,
The thread bacillus bacterium solution of 571g Rhodococcus sps bacterium solution, 571g butanedioic acids (i.e. proportionally 3:8:4:3:3) it is well mixed and obtains compound bacteria
Liquid.
Comparative example 1
A kind of high carbon-based additive of soil, is prepared by the following method to obtain：100kg soybean stalk is cut into 10~
Compound bacteria of the 30cm section shape as degraded raw material, thereto addition stalk weight 5%, addition 60kg water, fermentation process 3 days,
Turn over pushing away once；Turned over and push away once every 7 days again, turn over push away twice altogether, obtain the high carbon-based additive of the soil.
Wherein, the preparation method of the compound bacteria is：
S1. Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, the thread bacillus of butanedioic acid are lived respectively
Change, cultivate into bacterium solution viable bacteria Particle density as 0.8 × 109Individual/gram；
S2. take respectively 769g Phanerochaete chrysosporiums bacterium solution, 385g koning trichodermas bacterium solution, 1538g Paracoccus denitrificans bacterium solution,
The thread bacillus bacterium solution of 1923g Rhodococcus sps bacterium solution, 385g butanedioic acids (i.e. proportionally 2:1:4:5:1) it is well mixed and obtains compound bacteria
Liquid.
Comparative example 2 (comparison with embodiment 3, no Rhodococcus sp)
A kind of high carbon-based additive of soil, is prepared by the following method to obtain：100kg soybean stalk is cut into 10~
Compound bacteria of the 30cm section shape as degraded raw material, thereto addition stalk weight 2%, addition 40kg water, fermentation process 2 days,
Turn over pushing away once；Turned over and push away once every 12 days again, turn over push away twice altogether, obtain the high carbon-based additive of the soil.
Wherein, the preparation method of the compound bacteria is：
S1. Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, the thread bacillus of butanedioic acid are activated respectively, culture is extremely
Viable bacteria Particle density is 2 × 10 in bacterium solution9Individual/gram；
S2. take respectively 316g Phanerochaete chrysosporiums bacterium solution, 421g koning trichodermas bacterium solution, 632g Paracoccus denitrificans bacterium solution,
The thread bacillus bacterium solution of 632g butanedioic acids (i.e. proportionally 3:4:6:6) it is well mixed and obtains composite bacteria liquid.
Comparative example 3 (comparison with embodiment 3, no thread bacillus of butanedioic acid)
A kind of high carbon-based additive of soil, is prepared by the following method to obtain：100kg soybean stalk is cut into 10~
Compound bacteria of the 30cm section shape as degraded raw material, thereto addition stalk weight 2%, addition 40kg water, fermentation process 2 days,
Turn over pushing away once；Turned over and push away once every 12 days again, turn over push away twice altogether, obtain the high carbon-based additive of the soil.
Wherein, the preparation method of the compound bacteria is：
S1. Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp are activated respectively, cultivated into bacterium solution living
Bacteria concentration is 109Individual/gram；
S2. take respectively 400g Phanerochaete chrysosporiums bacterium solution, 533g koning trichodermas bacterium solution, 800g Paracoccus denitrificans bacterium solution,
267g Rhodococcus sps bacterium solution (i.e. proportionally 3:4:6:2) it is well mixed and obtains composite bacteria liquid.
Comparative example 4 (comparison with embodiment 3 is without Phanerochaete chrysosporium, koning trichoderma)
A kind of high carbon-based additive of soil, is prepared by the following method to obtain：100kg soybean stalk is cut into 10~
Compound bacteria of the 30cm section shape as degraded raw material, thereto addition stalk weight 2%, addition 40kg water, fermentation process 2 days,
Turn over pushing away once；Turned over and push away once every 12 days again, turn over push away twice altogether, obtain the high carbon-based additive of the soil.
Wherein, the preparation method of the compound bacteria is：
S1. Paracoccus denitrificans, Rhodococcus sp, the thread bacillus of butanedioic acid are activated respectively, cultivates into bacterium solution viable bacteria Particle density
For 109Individual/gram；
S2. 857g Paracoccus denitrificans bacterium solution, 286g Rhodococcus sps bacterium solution, the thread bacillus bacterium solution of 857g butanedioic acids are taken respectively (i.e.
Proportionally 6:2:6) it is well mixed and obtains composite bacteria liquid.
The inoculating proportion of comparative example 5
The method that this comparative example prepares the high carbon-based additive of soil is substantially same as Example 4, and difference is that this is right
Compound bacteria in ratio is using following several proportioning modes：Handle 1.-Phanerochaete chrysosporium：Koning trichoderma：Paracoccus denitrificans：
Rhodococcus sp：Thread bacillus=2 of butanedioic acid:2:7:4:9；Handle 2.-Phanerochaete chrysosporium：Koning trichoderma：Paracoccus denitrificans：It is red
Coccus：Thread bacillus=0.8 of butanedioic acid:9:4:3:1.5；Handle 3.-Phanerochaete chrysosporium：Koning trichoderma：Paracoccus denitrificans：
Rhodococcus sp：Thread bacillus=4 of butanedioic acid:2:2.5:4:7；Handle 4.-Phanerochaete chrysosporium：Koning trichoderma：Paracoccus denitrificans：
Rhodococcus sp：Thread bacillus=5 of butanedioic acid:2:2:4:9；Handle 5.-Phanerochaete chrysosporium：Koning trichoderma：Paracoccus denitrificans：It is red
Coccus：Thread bacillus=2 of butanedioic acid:2:3:2:1.
The compound bacteria addition of comparative example 6
The method that this comparative example prepares the high carbon-based additive of soil is substantially same as Example 2, and difference is compound
Bacterium uses following several processing compared to the addition of stalk：Handle 0.7% that 1.-compound bacteria is stalk weight；Processing 2.-it is multiple
Close 4.5% that bacterium is stalk weight；Handle 6% that 3.-compound bacteria is stalk weight；4.-compound bacteria is handled as stalk weight
8%.
Application Example
Test site：Guangdong Province Shaoguan Nanxiong City
Test period：2012~2013 years
Tobacco bred：K326
For trying soil：Beef liver soil field
Testing program：Each treatment group of embodiment and comparative example is experiment from 1 mu of growing way identical tobacco seedling
Object, and 3 parallel tests are set.Control treatment be reduce compared to normal tobacco cultivation management 25% dose；Implement
Example 1~5, to compare normal tobacco cultivation management, reduces by 25% dose, and add with each treatment group of comparative example 1~6
Add the 25% high carbon-based additive being prepared, other cultivation conditions are identical.The high carbon-based additive of various soil is determined to cigarette
The influence of grass growth, is harvested after tobacco maturation, determines the gross weight of the tobacco of each treatment group, also, the flue-cured tobacco that production obtains
Please the tobacco leaf expert that smokes panel test smoked panel test, determine the quality for the flue-cured tobacco that each treatment group obtains, as a result as shown in table 1.
Respectively processing harvests total amount and quality evaluation to table 1 to tobacco
The yield of flue-cured tobacco and quality of comparing embodiment 1~5 and comparative example 1, as a result as shown in table 1, it is known that compound bacteria adds
Dosage, each strain ratio, turn over and push away the time and can all influence the biology and physicochemical property of high carbon-based additive, using the high-carbon of the present invention
Base additive adds yield, quality and the output value of tobacco leaf etc. to a certain extent.
The yield of flue-cured tobacco and quality of comparing embodiment 3 and comparative example 2~4 can be seen that：One of inventive point of the present invention exists
Form compound bacteria in have selected mutually not antagonism, and by the strain of cooperative effect, the compound bacteria compared to by lignin, cellulose and
Degraded of the simple combination that hemicellulose degradation bacterium is carried out to soybean stalk is more effective.The thread bacillus of Rhodococcus sp, butanedioic acid, yellow spore
The flat lead fungi of raw wool and koning trichoderma are different to the influence degree of the yield of tobacco leaf, quality and the output value.Comparative example 2 is without Rhodococcus sp
When, the degradation effect of lignin weakens, and have impact on degraded of the compound bacteria subsequently to hemicellulose and cellulose, and the height is carbon-based adds
Thing is added to lose the repair that Rhodococcus sp is carried out to soil；Comparative example 3 is without the thread bacillus of butanedioic acid, the degradation effect of cellulose
It is obviously reduced；Comparative example 4 subtracts without Phanerochaete chrysosporium and koning trichoderma, lignin, cellulose, the degradation effect of pentosan
It is weak, and degraded of the compound bacteria subsequently to other materials of soybean stalk is have impact on, and the humus yield drop of the carbon-based additive of the height
It is low, varied bacteria growing, be unfavorable for improve tobacco yield and quality.
The tobacco harvest result for comparing 5 each processing mode of comparative example and embodiment 4 can be seen that：Another invention of the present invention
Point is that, to each strain progress reasonable compatibility, under conditions of rationally each strain ratio of compound bacteria is limited, tobacco could be improved
Yield and quality.Each strain proportion of compound bacteria of the present invention is found after many experiments, changes any strain
Between ratio, will all influence the property of soil additive that is prepared, and then the yield and quality of tobacco is had an impact.
The tobacco harvest result of 6 each processing mode of comparing embodiment 2 and comparative example can be seen that：The addition of compound bacteria
Total amount will be harvested to tobacco and quality evaluation has an impact.Generally, the addition of strain is the 1~10% of substrate, this
Invention is found, when the addition of compound bacteria is the 0.8~4% of soybean stalk weight, can improve the total amount and quality of tobacco, and right
Ratio 6 respectively handle tobacco total amount and quality it is relatively low.As can be seen here, the addition for controlling compound bacteria is soybean stalk weight
When 0.8~4%, the total amount and quality of tobacco can be effectively improved.
Claims (7)
1. a kind of application of the high carbon-based additive of soil that fermentation soybean stalk is prepared in tobacco planting soil is improved, its
It is characterised by, the additive is using soybean stalk as degraded raw material, and adds compound bacteria and is prepared, the compound bacteria is by such as
Each strain composition of lower ratio：Phanerochaete chrysosporium：Koning trichoderma：Paracoccus denitrificans：Rhodococcus sp：Thread bacillus=1 of butanedioic acid
~3:2~8:3~8:1~3:2~8；The addition of the compound bacteria is the 0.8 ~ 4% of soybean stalk weight.
2. application according to claim 1, it is characterised in that the compound bacteria is made up of each strain of following ratio：It is yellow
The flat lead fungi of archespore hair：Koning trichoderma：Paracoccus denitrificans：Rhodococcus sp：Thread bacillus=1 ~ 3 of butanedioic acid:4~8:5~8:2~3:4~8.
3. application according to claim 2, it is characterised in that the addition of the compound bacteria for soybean stalk weight 1 ~
2.5%。
4. application according to claim 1, it is characterised in that the preparation method of the high carbon-based additive of the soil is will be big
Beanstalk stalk cuts into 10 ~ 30cm section shape as degraded raw material, adds the compound bacteria of stalk weight 0.8 ~ 4% thereto, adds suitable
The water of amount, fermentation process turn over pushing away once after 2 ~ 3 days；Turned over and push away once every 8 ~ 15 days again, turn over push away twice altogether, obtain institute
State additive；Wherein, the preparation of the compound bacteria comprises the following steps：
S1. Phanerochaete chrysosporium, koning trichoderma, Paracoccus denitrificans, Rhodococcus sp, the thread bacillus of butanedioic acid are activated respectively, trained
Viable bacteria Particle density is supported into bacterium solution as 0.8 ~ 2 × 109Individual/gram；
S2. take the bacterium solution of above-mentioned five kinds of bacterium is well mixed to obtain composite bacteria liquid in proportion.
5. application according to claim 4, it is characterised in that the addition of the water is the 30 ~ 40% of stalk quality.
6. application according to claim 5, it is characterised in that each strain composition of the compound bacteria is yellow archespore Mao Pingge
Bacterium：Koning trichoderma：Paracoccus denitrificans：Rhodococcus sp：Thread bacillus=1 ~ 3 of butanedioic acid:4~8:5~8:2~3:When 4 ~ 8, during fermentation process
Between be 2 days, turn over pushing away once；Turned over and push away once every 8 ~ 10 days again, turn over push away twice altogether, obtain the additive.
7. application according to claim 1, it is characterised in that the application method of the high carbon-based additive of the soil is direct
Impose on field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510059562.8A CN104649759B (en) | 2015-02-04 | 2015-02-04 | The high carbon-based additive of soil that a kind of fermentation soybean stalk is prepared |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510059562.8A CN104649759B (en) | 2015-02-04 | 2015-02-04 | The high carbon-based additive of soil that a kind of fermentation soybean stalk is prepared |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104649759A CN104649759A (en) | 2015-05-27 |
| CN104649759B true CN104649759B (en) | 2018-04-10 |
Family
ID=53241468
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510059562.8A Expired - Fee Related CN104649759B (en) | 2015-02-04 | 2015-02-04 | The high carbon-based additive of soil that a kind of fermentation soybean stalk is prepared |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104649759B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109349050A (en) * | 2018-11-20 | 2019-02-19 | 河南农业大学 | A kind of cultural method for preventing and treating tobacco black shank |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102146341A (en) * | 2010-12-31 | 2011-08-10 | 东莞市天盛生物科技有限公司 | Straw decomposing inoculant |
| CN102174400A (en) * | 2011-01-27 | 2011-09-07 | 南昌大学 | Microbial degrading agent for decomposing cassava stalks and preparation method |
| CN102517217A (en) * | 2011-12-27 | 2012-06-27 | 何颖霞 | Environment-friendly straw fermenting agent |
| CN103642726A (en) * | 2013-11-28 | 2014-03-19 | 天津海友佳音生物科技股份有限公司 | Corn straw fermentation microorganism agent and preparation method and fermentation method thereof |
| CN104099281A (en) * | 2014-07-20 | 2014-10-15 | 尹梦珍 | Environment-friendly compound microbial agent |
-
2015
- 2015-02-04 CN CN201510059562.8A patent/CN104649759B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102146341A (en) * | 2010-12-31 | 2011-08-10 | 东莞市天盛生物科技有限公司 | Straw decomposing inoculant |
| CN102174400A (en) * | 2011-01-27 | 2011-09-07 | 南昌大学 | Microbial degrading agent for decomposing cassava stalks and preparation method |
| CN102517217A (en) * | 2011-12-27 | 2012-06-27 | 何颖霞 | Environment-friendly straw fermenting agent |
| CN103642726A (en) * | 2013-11-28 | 2014-03-19 | 天津海友佳音生物科技股份有限公司 | Corn straw fermentation microorganism agent and preparation method and fermentation method thereof |
| CN104099281A (en) * | 2014-07-20 | 2014-10-15 | 尹梦珍 | Environment-friendly compound microbial agent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104649759A (en) | 2015-05-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Owaid et al. | Applicable properties of the bio-fertilizer spent mushroom substrate in organic systems as a byproduct from the cultivation of Pleurotus spp | |
| CN104177137B (en) | A kind of microbial straw composing agent and preparation method thereof | |
| CN107201229B (en) | A kind of bioremediation agents and its preparation and application with improvement soil and regulating and controlling microbial population structure function | |
| Chanakya et al. | Cultivation of Pleurotus spp. on a combination of anaerobically digested plant material and various agro-residues | |
| CN105646071A (en) | Method for preparing biologic humic acid fertilizer from livestock manure | |
| CN104255420B (en) | A kind of seedling medium of melon diseases prevention growth promoting and preparation method thereof | |
| CN104909931A (en) | Cucumber seedling-raising substrate and preparation method thereof | |
| CN105481580A (en) | Novel organic fertilizer for kiwifruits and preparation method thereof | |
| CN102174400A (en) | Microbial degrading agent for decomposing cassava stalks and preparation method | |
| CN105948841B (en) | Organic fertilizer tank type fermentation method taking mushroom dregs as substrate | |
| CN102417424A (en) | Special biological organic fertilizer for traditional Chinese medicinal material isatis and preparation method thereof | |
| Zhou et al. | Cultivation of Pleurotus ostreatus, a potential candidate for biogas residues degradation | |
| CN102173899B (en) | Amino acid-containing liquid fertilizer and preparation method thereof | |
| CN101774849B (en) | Special organic fertilizer for cultivating herbaceous organic fruits and preparation method thereof | |
| Dedousi et al. | Utilization of spent substrates and waste products of mushroom cultivation to produce new crops of Pleurotus ostreatus, Pleurotus eryngii and Agaricus bisporus | |
| EP4271203A1 (en) | Plant material recycling inoculant and uses thereof | |
| CN105969697A (en) | Compound bacterium agent for composting straw as well as preparation method and application thereof | |
| CN103936517B (en) | A kind of saline land greening dedicated substrate and preparation method thereof | |
| CN113004077A (en) | Novel organic fertilizer and preparation method thereof | |
| CN104894025A (en) | Streptomyces sp. and application thereof | |
| Hammerschmiedt et al. | Assessment of digestates prepared from maize, legumes, and their mixed culture as soil amendments: Effects on plant biomass and soil properties | |
| CN103992956A (en) | Method for preparing trichoderma biocontrol microbial agent by using edible fungus chaff | |
| CN104649759B (en) | The high carbon-based additive of soil that a kind of fermentation soybean stalk is prepared | |
| CN106278550A (en) | A kind of universal cultivation organic substrate | |
| CN104649761B (en) | The high carbon-based additive of soil that a kind of rape stalk that ferments is prepared |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180410 Termination date: 20190204 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |