CN104621183A - Dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent, and preparation method and application thereof - Google Patents
Dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent, and preparation method and application thereof Download PDFInfo
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- CN104621183A CN104621183A CN201510084508.9A CN201510084508A CN104621183A CN 104621183 A CN104621183 A CN 104621183A CN 201510084508 A CN201510084508 A CN 201510084508A CN 104621183 A CN104621183 A CN 104621183A
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- polyhedrosis virus
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Abstract
The invention discloses a dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent, and a preparation method and application thereof. The dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent is prepared by soaking, chopping, eluting, filtering, centrifugally separating, drying in vacuum, blending and sub-packaging. A dendrolimus punctatus cytoplasmic polyhedrosis virus concentrated liquid is obtained by vacuum drying at 28-35 DEG C in negative 0.9 barometric pressure for 10-15 hours by virtue of a vacuum dryer; and the foul odor of the oiling agent is removed, so that a product is easy to popularize and apply; blending comprises the step of adding saponated glycerinum and analytically pure activated carbon; the content of a dendrolimus punctatus cytoplasmic polyhedrosis virus in the prepared oiling agent is 1500,000,000PIB/ml or above; the pathogenicity of the virus is high; and the expiration date of the oiling agent is prolonged and can reach 3-4 years. By virtue of the oiling agent disclosed by the invention, the infective rate of the dendrolimus punctatus cytoplasmic polyhedrosis virus can reach 95% or above; the oiling agent has a lasting control effect on pine moths in a forest for multiple years after prevention and treatment is performed once; and the lasting control effect can last for five years or above.
Description
Technical field
The invention belongs to microorganism formulation processing technique field, be specifically related to preparation method and the application of the viral insecticide finish of pine caterpillars control.
Background technology
Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus (Dendrolimus Cytoplasmic Polyhedrosis Virus, DCPV) be a kind of natural pathogen of pine caterpillars, Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus to control pine caterpillars have continue efficient, to features such as person poultry safety are nontoxic, and free from environmental pollution, without the need to three-protection design during production.Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is middle intestines infection type virus, only copies in host's midgut epithelial cell.The production process of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is actually to be separated this viral polyhedron in intestines in the pine caterpillars of infection Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus and separates with other solid matter (pine caterpillars body tissue fragment etc.) from polypide, then concentrated, add certain auxiliary agent and make formulation.
(patent No. is ZL03114048.3 to prior art " production method of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish ", CN1282416C, authorized announcement date on November 1st, 2006) comprise section, wash-out, filtration, sedimentation, centrifugation, mixture, detection, dilution, packing operation.Prepared finish solves easy precipitated and separated, blocks the problem of shower nozzle, meets the requirement of ultra low volume spraying, have killing rate high, free from environmental pollution, to person poultry safety, feature that production cost is low.But the pine caterpillars virus finish polyhedron content produced due to the method is low, and during woodland application, consumption is large; Shelf-life shorter (1-2), its viral 1-2 just loses activity, and this formulation still exists stench, peculiar smell is large, make operating personnel's work under bad environment, affect operating efficiency, be unfavorable for operator ' s health, hinder its produce on popularization and use.Therefore, these technical problems anxious to be resolved, more efficiently can promote the use of pine caterpillars viral product easily.
Summary of the invention
The object of the invention is to overcome defect that above-mentioned prior art exists and provide a kind of novel Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish and its preparation method and application, with the demand of large-scale application in satisfied production.
Technical scheme of the present invention is as follows:
1, a preparation method for Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish, comprise carry out successively immersion, chopping, wash-out filtration, centrifugation, vacuum drying, mixture, each step of packing, it is characterized in that:
Described immersion is: the pine caterpillars clear water infecting Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is soaked 14-16 days;
Described wash-out filters: the pine caterpillars of chopping after soaking is placed in agitator, after adding clear water stirring, proceed in dewaterer, with the rotating speed of 400-600 rev/min, coarse filtration liquid is obtained after dewaterer liquid outlet is with 20 order nylon gauze filters, then will, put into dewaterer at coarse filtration liquid, with the rotating speed of 400-600 rev/min, at dewaterer liquid outlet 120 mesh filter screens, coarse filtration liquid is filtered to obtain qualified filtrate;
Described centrifugation is: by qualified filtrate with seperator 6800 revs/min rotating speed be separated obtain Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate I;
Described vacuum drying is: Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate I is put into vacuum drier, between adjusting temperature 28-35 DEG C, under negative 0.9 atmospheric pressure, within vacuum drying 10-15 hour, obtains Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate II;
Described mixture is: batch can Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate II being placed in vacuum drier, the ratio adding 30 kilograms of saponification glycerine in every 100 kilograms of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrates II adds saponification glycerine, adding 500 grams of fineness in every 100 kilograms of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrates II is again that the ratio of the pure active carbon of analysis of more than 120 orders adds the pure active carbon of analysis that described fineness is more than 120 orders, shut batch can, start running vacuum drier, batch can sampling is opened by shutdown in operation process, under 600 times of biomicroscopes, counting chamber method is adopted to measure Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus content, when the content of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is 1,500,000,000 more than PIB/ml, shut down, namely packing obtains Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish.
2, the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish of preparation method's acquisition of a kind of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish described in technical scheme 1.
3, the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish of preparation method's acquisition of a kind of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish described in technical scheme 2 is in the application preventing and treating pine caterpillars.
4, according to technical scheme 3 a kind of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish preparation method obtain Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish control pine caterpillars application be along woodland on both sides of the road strip spray described Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish.
Compared with prior art, main innovate point of the present invention and beneficial effect are:
1, vacuum drying is adopted, go out the foul smell (vacuum drying under large negative pressure of existing Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish in a large number, make a large amount of fungus and bacterium inactivations in virus liquid, thus it is corrupt to make virus liquid stop, under negative pressure, a large amount of smell substances in virus liquid are extracted in vacuum drying out simultaneously, product peculiar smell is reduced greatly), the working environment making staff spray this finish obtains purification, under in order to be effective prerequisite, solve the problem that the products such as original viral product content is low, stench, peculiar smell are large are difficult to promote the use of.Meanwhile, process technology is extracted in pine caterpillars virus of the present invention vacuum drying, can remove a large amount of fungus and bacteriums in virus liquid, thus significantly improve the content of viral polyhedron.
2, adopt the mode adding active carbon, protect the activity of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, enhance the shelf-life of product, (saponification glycerine and active carbon all play a protective role to polyhedrosis virus to make produced finish shelf life of products reach 3-4, vacuum drying simultaneously makes moisture content reduce in a large number, reduce the hydrolysis of viral polyhedron, thus also make viral finish storage life extend), namely in this finish of 3-4, virus still has activity, and existing Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish is not owing to protecting the measure of this virus, Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish is made to see that light easily decomposes death, shelf-life only has 1-2.
3, the finish of the present invention's production, control pine caterpillars efficiency is high especially, and control (spraying) once can be protected pine forest more than 5 years pine caterpillars and not cause disaster, and reaches the effect of getting twice the result with half the effort.
The present invention adopts immersion, chopping, wash-out filters, centrifugation, vacuum drying, mixture virus protectant, product quality, the steps such as packing, the viral polyhedron content of the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish that the present invention is produced significantly improves, the pathogenicity of this preparation is obviously strengthened, in addition, the viral finish that the present invention produces obviously extended (anti-ultraviolet ability reinforcement) in the vigor phase of woodland, the contagiousness of this preparation virus is obviously strengthened, pine caterpillars virus susceptible gene just can be made to reach more than 95%, the pine caterpillars of this viral disease of infection is made to lose the infringement ability of infringement pine forest, after infecting the pine caterpillars of this virus then, dead worm falls within woodland, viral polyhedron can be preserved for many years in woods, 2nd year and all have lasting control effects to woodland pine caterpillars for many years afterwards, and also can be transmitted to the next generation by the pine caterpillars of this virus infections, therefore, the ULV Eliminating pine caterpillars efficiency that the inventive method is produced is high especially, only need spray once, in the 2nd year with all have lasting control effects to woodland pine caterpillars for many years afterwards, small part worm is only had in the 2nd year, within 3rd year, be difficult to find worm, thereafter continue to control for many years, the control efficiency of more than 5 years can be reached, reach the effect of getting twice the result with half the effort.In addition, this virus formulation shelf-life reaches 3-4, produces a collection of product, can use for many years, and significantly reduce the production cost of virus, what substantially increase product prevents and treats efficiency.And existing Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish due to viral polyhedron content low, and polyhedron is at the easy inactivation of woodland, and first time uses, only there is a small amount of pine caterpillars dead, therefore, this finish needs to spray more than 3 years continuously at woodland, just can reach the object controlling pine caterpillars; And the shelf-life only 1-2 of existing Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish, the shorter shelf-life makes the production cost of virus greatly increase.
In sum, the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish that the inventive method is obtained, eliminates stench peculiar smell, makes this product be easy to apply.The viral polyhedron content of the finish made is high, and pathogenicity is strong, and extend the shelf-life of finish, can guarantee the quality 3-4.Use finish of the present invention that pine caterpillars virus susceptible gene can be made to reach more than 95%, once, lasting control efficiency can reach more than 5 years in control.Overcome existing Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish polyhedron content low, and stench peculiar smell is large, staff's work under bad environment, hinder applying of finish, viral viability is low, needs repeatedly exhaustive the spraying of large area just effectively can prevent and treat pine caterpillars during use, use cost high, more difficult in woodland operation, the shelf-life only has 1-2, the defect that relative production cost is higher and deficiency.
When collecting the pine caterpillars infecting Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, the pine branch of the fresh needle of the band having sprayed Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus need be used to carry out nursing inoculation, this Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus (Dendrolimus Cytoplasmic polyhedrosis Virus, DCPV) be " production method of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish " in denomination of invention, the patent No. is ZL03114048.3, CN1282416C, the Chinese patent in authorized announcement date on November 1st, 2006 is open, the applicant Yunnan Academy of Forestry Sci. preserves above-mentioned biomaterial, applicant Yunnan Academy of Forestry Sci. ensures to provide to the public in 20 years from present patent application day.Address of the applicant: No. 2, blue gum road, Panlong District, Kunming, Yunnan Province, postcode: 650201.
Embodiment
Following embodiment is conventional method without specified otherwise.
Embodiment 1
1, the collection of the pine caterpillars of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is infected
(1) the large fence in field copies: the annual beginning of spring, and at the beginning of pine caterpillars overwinter generation causes harm comparatively serious 4 months, live about gathering for 4 ages pine caterpillars, puts into the large fence being in dead level set in advance.Fence arranges size and suits measures to local conditions, general 0.5-2.0 mu, in fence, put into that to have sprayed Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentration be that the pine branch of the fresh needle of band of the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus liquid of 3,000 ten thousand PIB/ milliliters carries out nursing inoculation, within 15-20 days, collect susceptible worm.The susceptible worm of collecting generally is transported to indoor processing with 50 kilograms of plastics barreleds to facilitate.The susceptible worm that field fence copies collection and obtains prepares Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish of the present invention by following immersion, chopping, wash-out filtration, centrifugation, vacuum drying, mixture, each step of packing successively.
(2) soak: the above-mentioned pine caterpillars clear water having infected caterpillar cytoplasmic polyhedrosis virus is soaked 14-16 days, soaking mainly in order to soak intestines in susceptible worm, below facilitating, cleaning out this viral polyhedron.
(3) shred: the above-mentioned susceptible worm through soaking is cut into 1-2 cm of tissue fragment.
(4) wash-out filters: the pine caterpillars of chopping is placed in agitator, add clear water and stir stirring after 7 minutes, proceed in dewaterer, at the rotating speed of 600 revs/min, after dewaterer liquid outlet is with 20 order nylon gauze filters, obtains coarse filtration liquid, large worm corpse tissue block is removed.Again coarse filtration liquid is filtered to obtain qualified filtrate at dewaterer liquid outlet 120 mesh filter screens again.
(5) centrifugation: by qualified filtrate with seperator 6800 revs/min rotating speed be separated obtain Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate I.
(6) vacuum drying: Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate is put into vacuum drier, between adjusting temperature 28-35 DEG C, under negative 0.9 atmospheric pressure, vacuum drying obtains Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate II in 10 hours.
(7) mixture: the batch can of SZG-250 type vacuum drier (manufacture of changzhou one step dryer factory) will be placed in through vacuum drying Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate II, the ratio adding 30 kilograms of saponification glycerine (commercially available) in every 100 kilograms of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrates II adds saponification glycerine, adding 500 grams of fineness in every 100 kilograms of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrates II is again that the ratio of the pure active carbon of analysis of more than 120 orders adds the pure active carbon of analysis that described fineness is more than 120 orders, shut batch can, start running vacuum drier, operate a hours, it is allowed fully to be mixed evenly, batch can sampling is opened by shutdown in operation process, under 600 times of biomicroscopes, counting chamber method is adopted to measure Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus content, during content 1,500,000,000 more than the PIB/ml of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, shut down, obtain Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish prepared by the inventive method.
(8) packing: by Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish 10 kilograms of Plastic Drum packing of content 1,500,000,000 more than the PIB/ml of above-mentioned Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus.
Embodiment 2
Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish of the present invention (hereinafter referred to as finish of the present invention) is in woodland large-scale application
(1) finish of the present invention is in Honghe state, Yunnan Province Milei County woodland large-scale application:
Huashan pine, pinus yunnanensis mix in raw natural forest in more than 10 ten thousand mu, Xi Yi township, Honghe state, Yunnan Province Milei County, cause harm by Dendrolimus kikuchii for years, injured area 38000 mu, cause part Huashan pine dead.Insect survey average population density in pine forest of being injured reaches 300-500 bar/strain.In mid-March, 2005, use finish of the present invention, finish consumption of the present invention 10 grams/acre, (finish is stoste to add water 2-3 kilogram in every mu of finish used, during use, suitable quantity of water can increase control area when ensureing control efficiency), use Japan to produce little Song power driven sprayer, the woodland road of the convenient walking along woodland highway both sides, strip sprays, and prevents and treats 20,000 mu.
Prevent and treat and check control efficiency after 25 days, control district's Dendrolimus kikuchii virus disease susceptible gene checks in March, 95%, 2006, and average population density drops to 10-20 bar/strain.In March, 2007 checks (first time sprays rear Second Year), be difficult to find worm, to 2014 (first time sprays latter 9th year), all there is not pine caterpillar in this sheet more than 10 ten thousand mu of natural forest, finish of the present invention sprays once, and pine caterpillars continues control effects and reaches 9 years.
(2) finish of the present invention is in mouth town, West Street, Shilin county of Kunming, Yunnan Province woodland large-scale application
West Street, Shilin county of Kunming, Yunnan Province Kou Zhen town government periphery about 30,000 mu of pinus yunnanensis Fast-growth phase are caused harm by Dendrolimus punctatus wenshanensis for many years, and be distributed with Duo Jia stone pit in pine forest, Long-Time Service chemical pesticide effectively cannot control the plague of insects, prevents every year, the plague of insects occurs every year.In mid-March, 2009, use ULV Eliminating of the present invention 4,000 mu, the finish consumption of use 10 grams/acre, add water in every mu of finish used 2-3 kilogram, uses Japan to produce little Song power driven sprayer, along the spraying of the strip on both sides of the road control of the convenient walking of woodland.
Prevent and treat and check control efficiency in latter 25 days, Dendrolimus punctatus wenshanensis virus disease susceptible gene 97% in control district; In April, 2010 checks control efficiency, occasionally has small part to set upper several Dendrolimus punctatus wenshanensis, adopts worm and carry out indoor detection, Virus carrying rate 78%; In March, 2011, cause harm the Sheng phase at Dendrolimus punctatus wenshanensis, in this sheet more than 30,000 mu forest land, carry out insect survey, cannot find a pine caterpillars; In March, 2014 is investigated in this forest zone again, still cannot find a pine caterpillars.
(3) finish of the present invention is in woods scenic area, Shilin county of Kunming, Yunnan Province periphery woodland large-scale application
Stone Forest Scenic Spot, Kunming, Yunnan Province is famous tourist attractions, and scenic spot periphery is more than 80,000 mu pinus yunnanensis Fast-growth phase of affiliated stone forest forest farm administration, and pine caterpillar is extremely serious, before 2009, will prevent and treat every year.Owing to there being the control efficiency of (2) part above, Shilin county gloomy control department has determined to prevent and treat 80,000 mu, stone forest forest farm Pinus Yunnanensis Forest finish of the present invention belonging to the periphery of Stone Forest Scenic Spot.In mid-March, 2009, use ULV Eliminating of the present invention 20,000 mu, the finish of use is 10 grams/acre, and add water in every mu of finish used 2-3 kilogram, uses Japan to produce little Song power driven sprayer, along the spraying of the strip on both sides of the road control of the convenient walking of woodland.
Prevent and treat and check control efficiency in latter 25 days, Dendrolimus punctatus wenshanensis virus disease susceptible gene 96% in control district; In April, 2012 checks control efficiency, occasionally has small part to set upper several Dendrolimus punctatus wenshanensis, adopts worm and carry out indoor detection, Virus carrying rate 71%; In mid-March, 2013 carries out insect survey in sheet more than 80,000 mu forest land belonging to stone forest forest farm, has been difficult to find pine caterpillars; In March, 2014 continues insect survey, cannot collect pine caterpillars.This county in history the most headachy pine caterpillar does not occur for continuous 5 years.
Claims (4)
1. a preparation method for Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish, comprises immersion, chopping, wash-out filtration, centrifugation, vacuum drying, mixture, each step of packing, it is characterized in that:
Described immersion is: the pine caterpillars clear water infecting Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is soaked 14-16 days;
Described wash-out filters: the pine caterpillars of chopping is placed in agitator, after adding clear water stirring, proceed in dewaterer, with the rotating speed of 400-600 rev/min, coarse filtration liquid is obtained after dewaterer liquid outlet is with 20 order nylon gauze filters, then will, put into dewaterer at coarse filtration liquid, with the rotating speed of 400-600 rev/min, at dewaterer liquid outlet 120 mesh filter screens, coarse filtration liquid is filtered to obtain qualified filtrate;
Described centrifugation is: by qualified filtrate with seperator 6800 revs/min rotating speed be separated obtain Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate I;
Described vacuum drying is: Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate I is put into vacuum drier, between adjusting temperature 28-35 DEG C, under negative 0.9 atmospheric pressure, within vacuum drying 10-15 hour, obtains Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate II;
Described mixture is: batch can Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrate II being placed in vacuum drier, the ratio adding 30 kilograms of saponification glycerine in every 100 kilograms of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrates II adds saponification glycerine, adding 500 grams of fineness in every 100 kilograms of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus concentrates II is again that the ratio of the pure active carbon of analysis of more than 120 orders adds the pure active carbon of analysis that described fineness is more than 120 orders, shut batch can, start running vacuum drier, batch can sampling is opened by shutdown in operation process, under 600 times of biomicroscopes, counting chamber method is adopted to measure Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus content, when the content of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is 1,500,000,000 more than PIB/ml, shut down, namely packing obtains Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish.
2. the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish of preparation method's acquisition of a kind of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish described in claim 1.
3. Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish according to claim 2 is in the application of control pine caterpillars.
4. Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish according to claim 3 control pine caterpillars application, it is characterized in that: along woodland on both sides of the road strip spray described Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish.
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| CN105613143A (en) * | 2015-12-18 | 2016-06-01 | 云南省林业科学院 | Method for controlling dendrolimus punctatus by using artificial migration natural enemies |
| CN110393185A (en) * | 2019-08-05 | 2019-11-01 | 云南省林业科学院 | The method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105613143A (en) * | 2015-12-18 | 2016-06-01 | 云南省林业科学院 | Method for controlling dendrolimus punctatus by using artificial migration natural enemies |
| CN110393185A (en) * | 2019-08-05 | 2019-11-01 | 云南省林业科学院 | The method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish |
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| CN104621183B (en) | 2017-07-07 |
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