CN110393185A - The method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish - Google Patents
The method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish Download PDFInfo
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- CN110393185A CN110393185A CN201910718047.4A CN201910718047A CN110393185A CN 110393185 A CN110393185 A CN 110393185A CN 201910718047 A CN201910718047 A CN 201910718047A CN 110393185 A CN110393185 A CN 110393185A
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- Prior art keywords
- polyhedrosis virus
- cytoplasmic polyhedrosis
- dendrolimus punctatus
- punctatus cytoplasmic
- concentration
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish, comprising the following steps: firstly, handling the pine moth for carrying Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, separates and be made the solution of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus；Secondly, the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus separated is concentrated, the concentrate of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is made.In the present invention, centrifugal treating is first passed through during concentration, and moisture is quickly gone out, in the high activity for guaranteeing Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus while highly concentrated by vacuum dehydrating at lower temperature, to in a disguised form improve the effective component in pulvis, concentrate is added in the concentrate of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus simultaneously, it dries again after sufficiently mixing, again by the product grind into powder after drying, wettable powder is made, compared to finish, holding time is longer and not volatile, and viral effective time extends, and deinsectization effect is more preferable.
Description
Technical field
The present invention relates to biological agent manufacture fields, the specially method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish.
Background technique
Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is middle intestines infection type virus, is only replicated in host's midgut epithelial cell.Pine tag
The production process of worm cytoplasmic polyhedrosis virus is actually in the pine moth of infection Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus in intestines
The viral polyhedron is separated from polypide and is separated with other solid matters (pine moth body tissue fragment etc.), then plus
With concentration, certain auxiliary agent is added, dosage form is made.
Technical solution in patent document CN104621183B is refined virus by concentration technique from pine moth virus
Out, and finish is made, but finds in practice, finish is readily volatilized in storage, transportational process, and then leads to having for virus
Time shortening is imitated, deinsectization effect is caused to reduce.
Summary of the invention
The purpose of the present invention is to provide the method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish, to solve above-mentioned background
Finish is proposed in technology in storage, transportational process, effective time that is readily volatilized and causing virus shortens, and causes deinsectization effect
The problem of reduction.
To achieve the above object, the invention provides the following technical scheme: the concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish
Method, comprising the following steps:
Firstly, handling the pine moth for carrying Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, separating and pine moth matter type being made and is more
The solution of angle precursor virus；
Secondly, the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus separated is concentrated, Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is made
Concentrate；
Then, auxiliary agent is added into the concentrate of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, and is uniformly mixed；
Finally, products therefrom in previous step is dried, and the dry product completed is pulverized into powder and is packed,
Obtain the wettable powder of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus.
Preferably, the concentrate of the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is the solution by Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus
It is made after centrifugal treating and vacuum dehydrating at lower temperature.
Preferably, the revolving speed when centrifugal treating is 7000r/min and the duration is 3min, and the vacuum and low temperature is dry
Vacuum degree when dry is negative under 0.8 atmospheric pressure and temperature is -10 DEG C, duration 12h.
Preferably, the addition auxiliary agent includes the sodium metnylene bis-naphthalene sulfonate for being used as diffusant, is used as anionic surface
The lignin sulfonic acid of activator, the diisobutyl sodium naphthalene sulfonate as wetting agent and filler.
Preferably, the mass percent of the sodium metnylene bis-naphthalene sulfonate is 5-15%, the quality hundred of the lignin sulfonic acid
Divide than being 25-28%, the mass percent of the diisobutyl sodium naphthalene sulfonate is 2-5%, and the mass percent of the filler is 1-
3%。
Preferably, it is stirred after the auxiliary agent addition by mixing plant, the revolving speed of mixing plant is 200r/min, is held
Continuous 5min.
Preferably, the concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is in the concentrate of the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus
60-65%, and when adding auxiliary agent, the concentrate of the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is room temperature.
Compared with prior art, the beneficial effects of the present invention are:
In the present invention, centrifugal treating is first passed through during concentration, moisture is quickly gone out, exist by vacuum dehydrating at lower temperature
While highly concentrated guarantee Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus high activity, thus in a disguised form improve pulvis in it is effective at
Point, while concentrate is added in the concentrate of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, it dries again after sufficiently mixing, then will do
Product grind into powder after dry, is made wettable powder, and compared to finish, the holding time is longer and not volatile, virus
Effective time extend, deinsectization effect is more preferable.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
A kind of embodiment provided by the invention: the method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish, including following step
It is rapid:
S101, firstly, handling the pine moth for carrying Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, separating and pine moth matter being made
The solution of type polyhedrosis virus；
S103, secondly, the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus separated is concentrated, pine moth cytoplasmic polyhedrosis is made
The concentrate of poison；
S105, then, auxiliary agent is added into the concentrate of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, and be uniformly mixed；
S107, finally, products therefrom in previous step is dried, and the dry product completed is pulverized into powder simultaneously
Pack, obtains the wettable powder of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus.
Wherein, the concentrate of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is the solution by Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus through being centrifuged
It is made after processing and vacuum dehydrating at lower temperature, revolving speed when centrifugal treating is 7000r/min and the duration is 3min, vacuum and low temperature
Vacuum degree when dry is negative under 0.8 atmospheric pressure and temperature is -10 DEG C, duration 12h, and vacuum drying eliminates pine moth
Peculiar smell in cytoplasmic polyhedrosis virus, while at low ambient temperatures, it keeps under low temperature environment, holding pine moth matter type as far as possible is more
The activity of angle precursor virus guarantees insecticidal effect.
Wherein, addition auxiliary agent includes the sodium metnylene bis-naphthalene sulfonate for being used as diffusant, is used as anionic surfactant
Lignin sulfonic acid, diisobutyl sodium naphthalene sulfonate and filler as wetting agent, the mass percent of sodium metnylene bis-naphthalene sulfonate
For 5-15%, the mass percent of lignin sulfonic acid is 25-28%, and the mass percent of diisobutyl sodium naphthalene sulfonate is 2-5%, is filled out
The mass percent of material is 1-3%, is stirred after auxiliary agent addition by mixing plant, and the revolving speed of mixing plant is 200r/min,
Continue 5min, the addition of each section auxiliary agent enables to pulvis to be quickly dissolved in water when in use and forms suspension, convenient for application work
The progress of work.
Wherein, the concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is 60- in the concentrate of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus
65%, and when adding auxiliary agent, the concentrate of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is room temperature, guarantees that pine moth matter type is more in pulvis
The content of angle precursor virus guarantees insecticidal effect so that the effective component in pulvis is enough.
Working principle: handling the pine moth for carrying Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, isolates pine moth matter
The solution of type polyhedrosis virus, then solution carried out highly concentrated, and contain the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus in concentrate
Amount reaches certain value, then adds sodium metnylene bis-naphthalene sulfonate, the lignin sulfonic acid as anionic surfactant, as wet
Moisten the auxiliary agents such as diisobutyl sodium naphthalene sulfonate and the filler of agent, after dry water removal, is developed into powder, can be prepared by pine moth matter type
The wettable powder of polyhedrosis virus.
Claims (7)
1. the method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish, which comprises the following steps:
Firstly, handling the pine moth for carrying Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, separating and pine moth matter type being made and is more
The solution of angle precursor virus；
Secondly, the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus separated is concentrated, Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is made
Concentrate；
Then, auxiliary agent is added into the concentrate of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus, and is uniformly mixed；
Finally, products therefrom in previous step is dried, and the dry product completed is pulverized into powder and is packed,
Obtain the wettable powder of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus.
2. the method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish according to claim 1, which is characterized in that the pine
The concentrate of caterpillar cytoplasmic polyhedrosis virus is the solution by Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus through centrifugal treating and vacuum and low temperature
It is made after drying.
3. the method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish according to claim 2, which is characterized in that it is described from
Revolving speed when heart processing is 7000r/min and the duration is 3min, and the vacuum degree when vacuum dehydrating at lower temperature is negative 0.8
Under atmospheric pressure and temperature is -10 DEG C, duration 12h.
4. the method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish according to claim 1, which is characterized in that described to add
Adding assistant includes the sodium metnylene bis-naphthalene sulfonate as diffusant, the lignin sulfonic acid as anionic surfactant, uses
Make the diisobutyl sodium naphthalene sulfonate and filler of wetting agent.
5. the method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish according to claim 4, which is characterized in that the Asia
The mass percent of methyl sodium dinaphthalenesulfonate is 5-15%, and the mass percent of the lignin sulfonic acid is 25-28%, and described two is different
The mass percent of sodium butylnaphthalenesulfonate is 2-5%, and the mass percent of the filler is 1-3%.
6. the method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish according to claim 1, which is characterized in that described to help
It is stirred after agent addition by mixing plant, the revolving speed of mixing plant is 200r/min, continues 5min.
7. the method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish according to claim 1, which is characterized in that the pine
The concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is 60-65% in the concentrate of caterpillar cytoplasmic polyhedrosis virus, and in addition auxiliary agent
When, the concentrate of the Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus is room temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910718047.4A CN110393185A (en) | 2019-08-05 | 2019-08-05 | The method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish |
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|---|---|---|---|
| CN201910718047.4A CN110393185A (en) | 2019-08-05 | 2019-08-05 | The method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish |
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| CN110393185A true CN110393185A (en) | 2019-11-01 |
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| CN201910718047.4A Pending CN110393185A (en) | 2019-08-05 | 2019-08-05 | The method for concentration of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus finish |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101396024A (en) * | 2008-11-04 | 2009-04-01 | 武汉武大绿洲生物技术有限公司 | Dendrolimus punctatus cytoplasmic polyhedrosis virus(dpcpv) bacillus thuringiensis wettable powder and preparation method thereof |
| CN104621183A (en) * | 2015-02-16 | 2015-05-20 | 云南省林业科学院 | Dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent, and preparation method and application thereof |
-
2019
- 2019-08-05 CN CN201910718047.4A patent/CN110393185A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101396024A (en) * | 2008-11-04 | 2009-04-01 | 武汉武大绿洲生物技术有限公司 | Dendrolimus punctatus cytoplasmic polyhedrosis virus(dpcpv) bacillus thuringiensis wettable powder and preparation method thereof |
| CN104621183A (en) * | 2015-02-16 | 2015-05-20 | 云南省林业科学院 | Dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent, and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 胡光辉 等: "松毛虫CPV 的增殖、提取及应用技术", 《云南林业科技》 * |
| 苏志远 等: "德昌松毛虫质型多角体病毒杀虫剂的生产工艺", 《四川林业科技》 * |
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