CN104620996A - Probiotic fermentation bed for breeding livestock and poultry - Google Patents
Probiotic fermentation bed for breeding livestock and poultry Download PDFInfo
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- CN104620996A CN104620996A CN201510109188.8A CN201510109188A CN104620996A CN 104620996 A CN104620996 A CN 104620996A CN 201510109188 A CN201510109188 A CN 201510109188A CN 104620996 A CN104620996 A CN 104620996A
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- fermentation
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- poultry
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K31/00—Housing birds
- A01K31/04—Dropping-boards; Devices for removing excrement
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a probiotic fermentation bed for breeding livestock and poultry, wherein after being subjected to microbial fermentation, organic bedding materials, such as Chinese herbal medicines, are prepared into a fermentation bed for breeding; livestock and poultry are put on the fermentation bed to breed; the problems of mould, pathogenic bacteria, parasite and the like can be solved; shit and urine excrements of the livestock and poultry can be degraded and digested rapidly and effectively; a breeding site is unnecessary to clean and wash every day; therefore, no waste is discharged from the breeding site; and the purpose of zero emission is achieved.
Description
Technical field
The present invention relates to livestock and poultry cultivation technical field, especially relate to a kind of probiotics fermention bed for livestock and poultry cultivation.
Background technology
In recent years along with aquaculture scale, business-like development, the producer is devoted to the raising of breeding performonce fo animals and quantity always, but does not cause enough attention on animal food safety and problem of environmental pollution.So that food security and Environment Pollution Event constantly occur.Though extremely country payes attention to, under the ordering about of advantage factors, effectively do not improved.Therefore, so that increasing people thirst for the national monitoring can strengthened food security and environmental pollution, and ensure that people can have the food of the relieved health of safety, current country has formulated a series of laws and regulations, but this all can not play the effect of effectively containment.
The environmental pollution that current China intensive culture causes and animal product residue problem more serious.The trace element mainly replenished in feed is mostly inorganic salt form, and because the mineral element abilities of digestive and absorption of animal body to inorganic states is poor, availability is low, so excess interpolation just must can play due effect.This is one, and it two is generally adopt high-copper (copper sulphate), high zinc (zinc oxide) and high antibiosis generally grain in Swine Production, to improve the productivity of animal.While causing data waste, environmental pollution, affect quality and the human health of animal product.
Barber etc. (1995) Late Cambrian adds the copper of animal normal needs 10 times in feed, can obviously improve its growth rate and food conversion.People also find that the zinc oxide of high dose can improve the daily ingestion amount of piglet and daily gain reduces feedstuff-meat ratio and grice diarrhoea rate, Feedstuff Enterprises and raiser generally add the even higher zinc oxide high dose trace element of 2000-3000mg/kg (high-copper and high zinc) as effective growth-promoting additive in piglet feed, have become pig industry nutrition regulation means comparatively widely.
In Swine Production, high-copper uses quite general as growth-promoting additive, due to the antagonism between trace element, also improve the consumption of the trace element such as zinc, iron simultaneously.Use in pig feed the inorganic microelements such as a large amount of copper (Cu), zinc (Zn), iron (Fe) due to absorption rate low, trace element is discharged in a large number through ight soil, causes severe contamination to environment (soil, water system etc.).In view of high dose trace element adds the harm caused, the European Community has proposed the sanitary standard of Feed Copper allowance.Specifying piggy 175mg/kg in 16 weeks, more than 17 weeks is 35mg/kg, and copper in daily ration and the maximum limitation of zinc respectively provide as 125mg/kg and 500mg/kg by Canada, U.S. feed association.China also adjusts feed medium trace element usage amount, piggy 200mg/kg, and growing and fattening pigs are 35mg/kg.High-copper not only can cause pig copper poisoning, also causes some nutrient (as VA, VD, VE, VB2) of pig to lack, when Feed Copper content is up to 250ppm, has bad shadow, thus cause food-safety problem to meat quality.Holland considers environmental protection, no longer allows in daily ration, use high-copper and high zinc as growth accelerator: Japan also strictly limits the consumption of copper, zinc.
Produce in the pollution of environment at Modern Animal Husbandry, the trace element such as copper, zinc accounts for the overwhelming majority.This not only causes animal micro-element nutrition surplus and waste economically, and causes severe contamination to ecotope.It is reported, the microelement additive that China uses every year is 15 ~ 180,000 tons, but due to its availability low, nearly about 100,000 tons not by the mineral matter of animal use with fecaluria discharge contaminated environment become large public hazards.In general feeds, the addition of inorganic microelement as copper is 20 ~ 40 times of body normal needs, after Feed Copper metabolism, 90% arranges through ight soil, copper is non-degradable material, and soil, water source, vegetation will inevitably be caused seriously dirty, hinders the development of environmental protection type agricultural.Zinc major part is from external source zinc unabsorbed in daily ration, and the research Pharmacological Level of Zinc daily ration group pig zinc every day discharge rate that shows to feed is 33 times of basic ration group of feeding, and the zinc of high dose like this enters in environment through defecate, and zinc in soil is accumulated and contaminated environment.If infer that the zinc in all pig manures rests in soil according to Japanese relevant expert, the Zn content contaminated soil of 120mg/kg that will exceed standard in 17 years no longer will have arability, and the zinc that zinc requires zinc excessive within 0.1mg/L to fish and the larger fishery water quality of hydrobiological toxicity also can hinder the sustainable development of livestock breeding by polluted underground water after carcass excretion
The micro-consumption of high dose like this, will certainly affect animal product quality, and copper tolerance limit (GB 15199-94) regulation in China's meat product, in pork, copper content is no more than 10ppm.High copper diet can cause pork liver copper content to increase 10-15 doubly, people can cause copper accumulation in the tissues such as liver, brain and kidney after eating the high animal product of cupric, the level of increase machine interior free yl, changes lipid metabolism, causes atherosclerotic and accelerate the aging and dead of cell.
From 1950's, animal scientists find, in feed, the antibiotic of low concentration can promote growth of animals or poultry significantly, and fodder antibiotics just widely uses, in the world each feed production state almost none does not add various antibiotic in feed.Since should saying nearly half a century, fodder antibiotics, to prevention Animal diseases, promotes growth of animal, improves the price of deed and really serves positive effect.20 Multiple Classes of Antibiotics and tens kinds of synthetic antimicrobial class medicines are had to be applied in feed according to statistics in the world.The sixties to the beginning of the eighties is the period that antibiotic should be with the most use by feedstuff industry.
As everyone knows, antibiotic has made tremendous contribution as the development of production promoter to livestock breeding.But the medicament residue caused by antibiotic use lack of standardization, the problems such as drug resistance attract great attention.For many years, because China raiser uses antibiotic for a long time in a large number, livestock and poultry profitable strain is killed, and cause body flora imbalance, suprainfection, drug resistance etc., immunologic function of livestock and birds and premunition, productivity decline.Aquaculture cost increases.Moreover, in the past in years, the expert of Europe and the U.S. just this has been investigation, and conclusion is that bacterium may be transferred to people to the tolerance of medicine in animal body.Drug resistance even passes to human body by animal products, brings threat to the health of the mankind, treatment.According to Chinese Veterinary Journal (1992), " rabid ox disease " of making us terrified in recent years is also that the three-in-one injection of one owing to making to water under beef injection antibiotic, hormone and sheep horse for a long time causes, and for example the generation of " pseudomembrane intestines, also known as antibiotic enteritis " also has substantial connection with abuse of antibiotics.
Antibiotic use not only suppresses or has killed pathogenic microorganism, also suppress or killed the beneficial bacterium in animal body simultaneously, break microecological balance, cause some hypovitaminosis of animal, immunocompetence reduces, even make non-sensitive flora and fungi amount reproduction, cause that the obstacle digested and assimilated is so that secondary infection, causes animal to fall ill or death.As Long-Time Service antibiotic, make livestock and poultry body produce dependence, limit the performance of immunocyte function in body, body's immunity is declined, resist the disease ability reduces.Antibiotic after animal alimentary canal plays a role, enters the blood circulation of animal with feed, and the filtration great majority through kidney excrete with urine, but still has some residual in animal body.These residual antibiotic are accumulated in animal product as meat, egg, milk, Mao Zhong, directly threaten health.Antibiotic is used likely to make animal produce gene mutation, deformity, carcinogenic; Antibiotic is discharged outside animal body in a large number, also may cause environmental pollution.According to reports indicate that, in feed, use the antibiotic of low dosage for a long time, the probability that drug resistance produces can be increased.
In view of the side effect that antibiotic is serious, EU Committee's legislation was forbidden in feed, add bacitracin, spiramycin, virginiamycin, tylosin 4 kinds of antibiotic from 1 day July in 1999.And be defined in the tissue of animal and must can not have detected medicament residue, this just has higher requirement to China's export animal products.At present, U.S. FDA eliminates penicillin, tetracycline, erythromycin, lincomycin, tylosin and virginiamycin from agricultural use.
In order to solve the problems such as livestock product safety hidden danger, scholars has carried out the research to green feed additive.Now existing probiotics and enzyme preparation, probiotics is the beneficial bacterium by increasing in digestive tract, suppresses harmful bacteria, and adjustment or maintenance gut flora balance, reach prevent disease, growth promoting effects, improve the effect of efficiency of feed utilization; Enzyme containing multiple decomposable asymmetric choice net feed Middle nutrition material in enzyme preparation, helps animal digestion to absorb, promotes growth of animal, improve efficiency of feed utilization.But these products can not reach the effect of fodder antibiotics completely.Chinese herbal medicine is the traditional medicine of preventing and curing diseases of China, and hope is sent in health care Chinese herbal medicine being used for animal by present people.Most research shows, Chinese herbal feed additive has and promotes growth of animals or poultry and health-care effect, and its toxic and side effect is little, noresidue, the unique advantage such as have no drug resistance, but Chinese medicine compound prescription amount is large, absorption difference, and extract is expensive, effect is single, application is restricted.
People obtains very large progress with field of medicaments to Effective Component of Chinese Medicine and micro-coordination research, along with developing rapidly and further investigation to Chinese medicine physiology, pharmacological active substance of bioinorganic chemistry, a large amount of achievements in research using active ingredient important in Chinese medicine for trace element (inorganic constituents) and the significance to life, health and curative effect, has given sufficient identification.To the research of trace element also by simple mensuration, progressively deeply.Show there is stronger pharmacologically active after trace element coordinates with organic principle in Chinese herbal medicine according to Effective Component of Chinese Medicine and micro-coordination theory research, some is also higher than organic compound for its activity.Pharmacology effectiveness is combining form mostly, easilier than alone mineral salt penetrates in tissue.For plant extracts and microelement complex the application of feedstuff industry domestic there is not yet to study report.
Along with the enhancing of global food awareness of safety, under current cultivation condition, how while performance animal largest production performance, the health of animal can be ensured again, improve body immunity and the resistance etc. of the foeign elements such as disease is more and more become to the core content of Production of Livestock and Poultry.So our company coordinates a kind of NEW TYPE OF COMPOSITE poultry micro-ecological preparation of trace element and Chinese herbal medicine fermentation by research application probio, in order to substitute antibiotics, reduce micro-consumption simultaneously.This product, by changing intestinal microflora, overcomes the adverse consequences of antibiotic and chemicals, has growth promotion, prevents and cures diseases, the effect such as health care, can significantly improve livestock and poultry production performance, improve efficiency of feed utilization etc.This can not only strengthen safe animal product and produce and environmental protection, ensures people's health, and can promote sustainable development of animal husbandry again, meeting the needs of China's Foreign Trade, is also the needs of national politics economic stability simultaneously.
In the prior art, livestock and poultry cultivation creates a large amount of ight soil, containing a large amount of nitrogen, phosphorus and organic pollutant etc. in feces of livestock and poultry, the pernicious gases such as a large amount of ammonia nitrogens, hydrogen sulphide, scatol, methane can be produced afterwards by fermentation, these gases not only can destroy ecology, but also directly can affect human health.Livestock and poultry farm (family) environmental consciousness is poor in addition, and arbitrarily stack feces of livestock and poultry and sewage effluent, dirt enters water system with rainwater and sewage, severe contamination underground water, causes soil property and water quality constantly to worsen.Since reform and opening-up, China's livestock breeding achieves significant progress, and the production of meat of current China leaps to the first in the world, and has become genuine herding big country.But livestock breeding remains low yield industry in China, for improving the productivity of animal, multiplex and abuse feed addictive (copper, zinc, antibiotic and the class additive that promotes production) has become the ubiquitous problem of feedstuff industry.Country has also put into effect some corresponding laws and regulations and has been limited, but it is not fully up to expectations to produce effects.These additive parts remain in the middle of animal product, by the edible health directly affecting people; The direct entered environment of part follower excreta, causes certain pollution to soil, air, water source, and then enters human body by food chain, same potential threat human health.Therefore, how to solve livestock breeding safety in production and problem demanding prompt solution in current sustainable development of animal husbandry has been become on the impact of environment.Along with China's rapid development of economy, the popularization of the ecological agriculture, the demand of feedstuff industry to environmentally friendly machine is increasing.
Summary of the invention
The technical problem that the present invention solves is exactly, a kind of probiotics fermention bed for livestock and poultry cultivation is provided, it is made into the fermentation bed cultivated after organic bedding and padding such as Chinese herbal medicine are carried out fermentable, by cultivation placed on it for livestock and poultry, mould, pathogenic bacteria can be solved, the problems such as parasitic generation; Can to degrade quickly and effectively, digest the fecaluria excreta of livestock and poultry, not need clean every day and rinse farming site, thus do not have any discarded object to discharge farming site, reach the object of zero-emission.
The technical solution used in the present invention is:
For a probiotics fermention bed for livestock and poultry cultivation, its preparation method comprises:
1. probiotics fermentation: comprise the anaerobic fermentation of anaerobe and the aerobic fermentation of aerobic microorganisms; Described anaerobe comprises plant lactobacillus and enterococcus faecalis, and described aerobic microorganisms comprises Geotrichum, S. cervisiae, actinomycetes and bacillus subtilis; Described anaerobic fermentation is inoculated in liquid nutrient medium by anaerobe to carry out anaerobic constant temperature culture, and obtain anaerobic fermentation bacterium liquid by one-level expansion cultivation and secondary expansion cultivation; Described aerobic fermentation is inoculated in liquid nutrient medium by aerobic microorganisms to carry out aerobic constant temperature cultivation, and obtain aerobic fermentation bacterium liquid by one-level expansion cultivation and secondary expansion cultivation;
2. the preparation of probiotics fermention bed: the various raw materials of preparation fermentation bed material are mixed, obtain organic bedding and padding, again anaerobic fermentation bacterium liquid and aerobic fermentation bacterium liquid are seeded in organic bedding and padding, carry out accumulation solid state fermentation, after 3-5 days when solid material temperature reaches 50 DEG C, organic bedding and padding are evenly paved into described fermentation bed, spread thickness and be not less than 60cm, then livestock and poultry to be cultivated; Described organic bedding and padding comprise sawdust, rice husk, wheat bran and the first compound Chinese herb, and described first compound Chinese herb comprises dandelion, cordate houttuynia, oriental wormwood, the bark of ash, folium isatidis, barrenwort, sweet wormwood, Herba Andrographitis, black nightshade, macleaya cordata, wolf's milk, rhizome of cyrtomium and Golden Larch Bark.
Preferably, step 1. described in the detailed process of anaerobic fermentation be the plant lactobacillus that will preserve, enterococcus faecalis is inoculated in liquid nutrient medium, 37 DEG C of constant temperature culture 24h under anaerobic, as zymotic fluid pH < 4.5, cultivation is expanded by carrying out one-level in its culture transferring to the fermentation tank of 50L, 37 DEG C of constant temperature culture 24h under anaerobic, as zymotic fluid pH < 4.5 in fermentation tank, cultivation is expanded by carrying out secondary in its culture transferring to the fermentation tank of 1000L, the lower 37 DEG C of constant temperature culture 30h of oxygen free condition, as zymotic fluid pH < 4.5 in fermentation tank, terminate fermentation, obtain described anaerobic fermentation bacterium liquid.
Preferably, step 1. described in the detailed process of aerobic fermentation be that described aerobic microorganisms is inoculated in liquid nutrient medium, the lower 30 DEG C of constant temperature culture 24h of aerobic conditions, as zymotic fluid pH < 5.5, cultivation is expanded by carrying out one-level in its culture transferring to the fermentation tank of 50L, 30 DEG C of constant temperature culture 24h under aerobic conditions, when in tank during zymotic fluid pH < 5.5, cultivation is expanded by carrying out secondary in its culture transferring to the fermentation tank of 1000L, 30 DEG C of constant temperature culture 30h under aerobic conditions, when in fermentation tank during zymotic fluid pH < 5.5, terminate fermentation, obtain described aerobic fermentation bacterium liquid.
Preferably, the composition of liquid nutrient medium used in described anaerobic fermentation and aerobic fermentation comprises: glucose, sulfate of ammoniac, manganese sulphate, magnesium sulfate, potassium dihydrogen phosphate, corn, dregs of beans and deionized water.
Preferably, step 2. described in the composition of organic bedding and padding and parts by weight thereof comprise 40% sawdust, the rice husk of 40%, the wheat bran of 5% and 15% the first compound Chinese herb, in described first compound Chinese herb, the parts by weight of various composition are: dandelion 1-2 part, cordate houttuynia 1-2 part, oriental wormwood 1-2 part, bark of ash 1-2 part, folium isatidis 1-2 part, barrenwort 1-2 part, sweet wormwood 1-2 part, Herba Andrographitis 1-2 part, black nightshade 1-2 part, macleaya cordata 2-4 part, wolf's milk 1-2 part, rhizome of cyrtomium 1-3 part and Golden Larch Bark 1-4 part.
Preferably, step 2. described in anaerobic fermentation bacterium liquid and aerobic fermentation bacterium liquid be mix with the volume ratio of 1:1, then to be seeded in organic bedding and padding by the inoculum concentration of 10% (mass percent).
Preferably, the water content of postvaccinal described organic bedding and padding is no more than 60%.
Preferably, step 2. described in the size of fermentation bed for being not less than 20m
2, described livestock and poultry are pig, and on average every pig area occupied is 1.0 ~ 1.5m
2.
Key technology point of the present invention is by being fermented by organic bedding and padding such as Chinese herbal medicine, and be made into fermentation bed livestock and poultry are cultivated, probiotics fermention bed of the present invention makes up existing ecological bed shortcoming by Chinese herbal medicine with ecological bed being combined, solve mould, pathogenic bacteria, the problems such as parasitic generation; Be particularly useful for pig cultivation, the present invention adopts the ecological cultivation method of probiotics fermention bed thoroughly to solve the pollution problem of raising pigs to environment.Due in organic bedding and padding of spreading in described fermentation bed containing the activated beneficial microbe of tool (namely above-mentioned several bacterial classifications), it can be degraded quickly and effectively, digest the fecaluria excreta of pig, do not need clean swinery every day, rinse pig house, thus do not have any discarded object to discharge pig farm, also without any the sewage rinsing colony house, more than 70% can be conserved water.Really reach the object of zero-emission of raising pigs.Improve pig house environment, the hog house design with fermentation bed is large window simultaneously, and Full-open in summer Rolling curtain closet in winter is incubated, and pig house roof arranges electric exhausting skylight, ventilated good, growth that temp. and humidity is all suitable for pig.Pig dung and urine decomposes rapidly through beneficial microbe, there is no foul smell in pig house, do not grow fly, detect through surrounding air, in microecological fermentation bed pig house, the pernicious gas content such as carbonic acid gas, hydrogen sulphide, ammonia is all lower than traditional brooder pig house, is significantly less than National agricultural portion " animal or fowls farm environment matter dish standard ".In addition, the first compound Chinese herb powder is used in the fermentation bed of Tiny ecosystem, fermentation bed is further improved, to prevent mould growth, pathogenic bacteria and parasite etc., greatly reduce the incidence of disease of pig, efficiently solve when pig farm and swinery generation respiratory disease, if not medication, growth speed of pigs is slow, survival rate and growth rate is low, the price of deed reduce, the deficiency that interests lose more than gain.
Embodiment
Hereinafter will be described in detail to embodiments of the invention.It should be noted that, when not conflicting, the embodiment in the application and the feature in embodiment can be combined mutually.
Embodiment 1: the kind of probiotics and fermentation process thereof
This fermentation bed uses bacterial classification to comprise plant lactobacillus, enterococcus faecalis, Geotrichum, S. cervisiae, actinomycetes and bacillus subtilis, and plant lactobacillus and enterococcus faecalis are carried out anaerobic fermentation, and other bacterial classification carries out aerobic fermentation.
Anaerobic fermentation: intact plant lactobacillus and enterococcus faecalis to be inoculated in liquid nutrient medium (1000mL conical flask loading amount is generally 500mL) under anaerobic environment, i.e. 37 DEG C of constant temperature culture 24h under anaerobic, as zymotic fluid pH < 4.5, cultivation is expanded by carrying out one-level in its culture transferring to the fermentation tank of 50L, then 37 DEG C of constant temperature culture 24h under anaerobism (i.e. anaerobic) condition, as zymotic fluid pH < 4.5 in fermentation tank, cultivation is expanded again by carrying out secondary in its culture transferring to the fermentation tank of 1000L, again under anaerobic, 37 DEG C of constant temperature culture 30h, as zymotic fluid pH < 4.5 in fermentation tank, liquid fermentation terminates, obtain the bacterium liquid of anaerobic fermentation.
Aerobic fermentation: by the Geotrichum preserved, S. cervisiae, actinomycetes and bacillus subtilis are inoculated into (1000mL conical flask loading amount is 500mL) in liquid nutrient medium, under aerobic environment, 30 DEG C of constant temperature culture 24h, as zymotic fluid pH < 5.5, cultivation is expanded by carrying out one-level in its culture transferring to the fermentation tank of 50L, then under aerobic conditions, 30 DEG C of constant temperature culture 24h, as zymotic fluid pH < 5.5 in fermentation tank, cultivation is expanded by carrying out secondary in its culture transferring to the fermentation tank of 1000L, under aerobic conditions, 30 DEG C of constant temperature culture 30h, when in fermentation tank during zymotic fluid pH < 5.5, liquid fermentation terminates, obtain the bacterium liquid of aerobic fermentation.
Preferably, the composition of liquid nutrient medium used in above-mentioned anaerobic fermentation and aerobic fermentation comprises: the compositions such as glucose, sulfate of ammoniac, manganese sulphate, magnesium sulfate, potassium dihydrogen phosphate, corn, dregs of beans and deionized water.
Embodiment 2: preparation fermentation bed
First percentage by weight is respectively the sawdust of 40%, the rice husk of 40%, the wheat bran of 5%, first compound Chinese herb of 15% mixes, be organic bedding and padding, again the bacterium liquid of anaerobic fermentation and the bacterium liquid of aerobic fermentation are mixed with volume ratio 1:1, mixed bacterium liquid is seeded in described organic bedding and padding by the inoculum concentration by 10%, water content control is being no more than 60%, then solid state fermentation is carried out in the mode of piling up, after 3-5 days when solid material temperature reaches 50 DEG C, organic bedding and padding after fermentation are not less than 60cm with thickness be layered on and be not less than in the fermentation bed of 20m2, ecologic breeding is carried out to pig, density is that on average every pig accounts for 1.0m2 ~ 1.5m2.This first compound Chinese herb is 80 object powder.
The present invention adopts the ecological cultivation method of probiotics fermention bed thoroughly to solve the pollution problem of raising pigs to environment.Due in organic bedding and padding of spreading in above-mentioned fermentation bed containing the activated beneficial microbe of tool (namely above-mentioned several bacterial classifications), it can be degraded quickly and effectively, digest the fecaluria excreta of pig, do not need clean swinery every day, rinse pig house, thus do not have any discarded object to discharge pig farm, also without any the sewage rinsing colony house, more than 70% can be conserved water.Really reach the object of zero-emission of raising pigs.Improve pig house environment, because being large window with the hog house design of fermentation bed, Full-open in summer Rolling curtain closet in winter is incubated, and pig house roof arranges electric exhausting skylight simultaneously, ventilated good, growth that temp. and humidity is all suitable for pig.Pig dung and urine decomposes rapidly through beneficial microbe, there is no foul smell in pig house, do not grow fly, detect through surrounding air, in microecological fermentation bed pig house, the pernicious gas content such as carbonic acid gas, hydrogen sulphide, ammonia is all lower than traditional brooder pig house, is significantly less than National agricultural portion " animal or fowls farm environment matter dish standard ".In addition, the first compound Chinese herb powder is used in the fermentation bed of Tiny ecosystem, fermentation bed is further improved, to prevent mould growth, pathogenic bacteria and parasite etc., greatly reduce the incidence of disease of pig, efficiently solve when pig farm and swinery generation respiratory disease, if not medication, growth speed of pigs is slow, survival rate and growth rate is low, the price of deed reduce, the deficiency that interests lose more than gain.
In addition, it should be noted that be provided with in fermentation bed to raise pig pig house can not use chemical disinfection medicine and antibiotics at this, if used, will kill and suppress microorganism, or suppressing it to breed, the activity of microorganism is reduced; This is also conducive to preventing people from using the materials such as antibiotic to raise pig.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1., for a probiotics fermention bed for livestock and poultry cultivation, it is characterized in that: preparation method comprises:
(1) probiotics fermentation: comprise the anaerobic fermentation of anaerobe and the aerobic fermentation of aerobic microorganisms; Described anaerobe comprises plant lactobacillus and enterococcus faecalis, and described aerobic microorganisms comprises Geotrichum, S. cervisiae, actinomycetes and bacillus subtilis; Described anaerobic fermentation is inoculated in liquid nutrient medium by anaerobe to carry out anaerobic constant temperature culture, and obtain anaerobic fermentation bacterium liquid by one-level expansion cultivation and secondary expansion cultivation; Described aerobic fermentation is inoculated in liquid nutrient medium by aerobic microorganisms to carry out aerobic constant temperature cultivation, and obtain aerobic fermentation bacterium liquid by one-level expansion cultivation and secondary expansion cultivation;
(2) probiotics fermention bed is prepared: mixed by the various raw materials of preparation fermentation bed material, obtain organic bedding and padding, again the anaerobic fermentation bacterium liquid in step (1) and aerobic fermentation bacterium liquid are seeded in organic bedding and padding, carry out accumulation solid state fermentation, after 3-5 days when solid material temperature reaches 50 DEG C, by organic bedding and padding uniform spreading on livestock and poultry cultivation bed, spread thickness and be not less than 60cm, make described probiotics fermention bed, then cultivate placed on it for livestock and poultry; Described organic bedding and padding comprise sawdust, rice husk, wheat bran and the first compound Chinese herb, and described first compound Chinese herb comprises dandelion, cordate houttuynia, oriental wormwood, the bark of ash, folium isatidis, barrenwort, sweet wormwood, Herba Andrographitis, black nightshade, macleaya cordata, wolf's milk, rhizome of cyrtomium and Golden Larch Bark.
2. probiotics fermention bed according to claim 1, it is characterized in that: the detailed process of anaerobic fermentation described in step (1) is the plant lactobacillus that will preserve, enterococcus faecalis is inoculated in liquid nutrient medium, 37 DEG C of constant temperature culture 24h under anaerobic, as zymotic fluid pH < 4.5, cultivation is expanded by carrying out one-level in its culture transferring to the fermentation tank of 50L, 37 DEG C of constant temperature culture 24h under anaerobic, as zymotic fluid pH < 4.5 in fermentation tank, cultivation is expanded by carrying out secondary in its culture transferring to the fermentation tank of 1000L, the lower 37 DEG C of constant temperature culture 30h of oxygen free condition, as zymotic fluid pH < 4.5 in fermentation tank, terminate fermentation, obtain described anaerobic fermentation bacterium liquid.
3. probiotics fermention bed according to claim 1, it is characterized in that: the detailed process of the aerobic fermentation described in step (1) is inoculated in liquid nutrient medium by described aerobic microorganisms, the lower 30 DEG C of constant temperature culture 24h of aerobic conditions, as zymotic fluid pH < 5.5, cultivation is expanded by carrying out one-level in its culture transferring to the fermentation tank of 50L, 30 DEG C of constant temperature culture 24h under aerobic conditions, when in tank during zymotic fluid pH < 5.5, cultivation is expanded by carrying out secondary in its culture transferring to the fermentation tank of 1000L, 30 DEG C of constant temperature culture 30h under aerobic conditions, when in fermentation tank during zymotic fluid pH < 5.5, terminate fermentation, obtain described aerobic fermentation bacterium liquid.
4. probiotics fermention bed according to claim 1, is characterized in that: the composition of liquid nutrient medium used in described anaerobic fermentation and aerobic fermentation comprises: glucose, sulfate of ammoniac, manganese sulphate, magnesium sulfate, potassium dihydrogen phosphate, corn, dregs of beans and deionized water.
5. probiotics fermention bed according to claim 1, it is characterized in that: the composition of organic bedding and padding described in step (2) and parts by weight thereof: comprise the sawdust of 40%, the rice husk of 40%, the wheat bran of 5% and first compound Chinese herb of 15%, in described first compound Chinese herb, the parts by weight of various composition are: dandelion 1-2 part, cordate houttuynia 1-2 part, oriental wormwood 1-2 part, bark of ash 1-2 part, folium isatidis 1-2 part, barrenwort 1-2 part, sweet wormwood 1-2 part, Herba Andrographitis 1-2 part, black nightshade 1-2 part, macleaya cordata 2-4 part, wolf's milk 1-2 part, rhizome of cyrtomium 1-3 part and Golden Larch Bark 1-4 part.
6. probiotics fermention bed according to claim 1, it is characterized in that: the anaerobic fermentation bacterium liquid described in step (2) and aerobic fermentation bacterium liquid mix with the volume ratio of 1:1, then be seeded in organic bedding and padding by the inoculum concentration of mass percent 10%.
7. probiotics fermention bed according to claim 1, is characterized in that: in step (2), the water content of postvaccinal described organic bedding and padding is no more than 60%.
8. probiotics fermention bed according to claim 1, is characterized in that: the size of the probiotics fermention bed described in step (2) is for being not less than 20m
2, described livestock and poultry are pig, and on average every pig area occupied is 1.0 ~ 1.5m
2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510109188.8A CN104620996A (en) | 2014-04-17 | 2015-03-12 | Probiotic fermentation bed for breeding livestock and poultry |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410155490.2A CN103891674A (en) | 2014-04-17 | 2014-04-17 | Ecological cultivation method with health care fermented feed matched with probiotic fermentation bed |
| CN2014101554902 | 2014-04-17 | ||
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| CN104946565A (en) * | 2015-06-29 | 2015-09-30 | 浙江惠嘉生物科技有限公司 | Solid-state continuous fermentation method of Bacillus subtilis |
| CN104885952A (en) * | 2015-07-08 | 2015-09-09 | 青岛嘉瑞生物技术有限公司 | Fermentation bed padding for ecologically breeding sheep |
| CN106069869A (en) * | 2016-07-01 | 2016-11-09 | 张雨 | A kind of sterilization bedding and padding for breeding layer chicken field |
| CN114051940A (en) * | 2021-12-07 | 2022-02-18 | 贵州天能生物科技有限公司 | Cattle breeding fermentation bed and method for preparing organic fertilizer by fermenting cattle manure |
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| CN104642259A (en) | 2015-05-27 |
| CN104686847A (en) | 2015-06-10 |
| CN104761310A (en) | 2015-07-08 |
| CN103891674A (en) | 2014-07-02 |
| CN104642259B (en) | 2017-11-03 |
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