CN104585019B - The selection of the long fringe neck CMS line of erect head long stigma - Google Patents
The selection of the long fringe neck CMS line of erect head long stigma Download PDFInfo
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- CN104585019B CN104585019B CN201510019839.4A CN201510019839A CN104585019B CN 104585019 B CN104585019 B CN 104585019B CN 201510019839 A CN201510019839 A CN 201510019839A CN 104585019 B CN104585019 B CN 104585019B
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Abstract
The invention discloses the selection of the long fringe neck CMS line of erect head long stigma, belong to rice sterile line breeding technical field. the present invention selects erect head rice varieties Shennong-265, long stigma rice varieties sea B long fringe neck rice varieties IR68902eB1 provide parent for objective trait, it is keep system for recurrent parent to produce the bigger excellence of upper popularizing area three, by hybridizing, reestablish diplomatic relations and backcross transformation, it is bred as economical character, yield traits is close with former sterile line, there is erect head simultaneously, the CMS line of long stigma and long fringe neck character, this sterile line not Bao Sui, stigma exposing ratio is high, outcrossing seed-setting rate is high, the hybrid seed yield of hybrid paddy rice can be improved with its preparation ternary hybrid rice, reduce production cost and reduce environmental pollution.
Description
Technical field
Present invention relates particularly to the selection of the long fringe neck CMS line of erect head long stigma, belong to rice sterile line breeding technical field.
Background technology
The incubation of breeding of short stem and hybrid rice is twice great leap in China's Oryza sativa L. scientific and technological revolution, makes the rice yield of China be greatly improved, substantially solves the problem of food and clothing of national people. But produce the sterile line of the ternary hybrid rice of upper popularization and application at present, bag neck bag fringe phenomenon is serious, in seed produces, need to spray gibberellins (being commonly called as 920) solve bag fringe problem, but spray gibberellins and not only increase the cost that seed produces, also create environmental pollution and seed vigor declines, and the long-spike neck sterile line containing eui gene can reach heredity and release bag neck Bao Sui, in the production of hybrid seeds, sterile line can use no or little gibberellins, so that the seed production cost of hybrid paddy rice declines, seed quality improves and reduces environmental pollution. The rice panicle type group structure of erect head type is reasonable, and the ecological condition such as canopy intensity of illumination, temperature, humidity is superior, is conducive to improving fertilizer resistance anti-lodging, also helps pollen transmission, improves outcrossing seed-setting rate. The length size of sterile line grain husk chapiter directly influences its complexity accepting pollen, and the sterile line of long stigma is conducive to male parent to pollinate because stigma exposing ratio is high, and hybrid seed yield is also high than common little stigma sterile line. These 3 kinds of character being conducive to outcrossing to be pollinated of erect head, long stigma and long fringe neck be polymerized imports in CMS line simultaneously, the CMS line being bred as has that not Bao Sui, outcrossing seed-setting rate be high, hybrid seed yield advantages of higher, also can reduce labour cost, reduce environmental pollution, improve peasant's production of hybrid seeds benefit.
Summary of the invention
It is an object of the invention to provide the selection of the long fringe neck CMS line of a kind of erect head long stigma, thus improving outcrossing seed-setting rate and the hybrid seed yield of CMS line. the present invention selects erect head rice varieties Shennong-265, B(Chen Yu is new in long stigma rice varieties sea. the election effects of hybrid rice long stigma sterile line-Hai A. and the Journal of Hainan University's natural science edition, 1991, 9 (2): 54-58), long fringe neck rice varieties IR68902eB1 provides parent for objective trait, it is keep system for recurrent parent to produce the bigger excellence of upper popularizing area three, by hybridizing, reestablish diplomatic relations and backcross transformation, it is bred as economical character, yield traits is close with former sterile line, there is erect head simultaneously, the CMS line of long stigma and long fringe neck character, this sterile line not Bao Sui, stigma exposing ratio is high, outcrossing seed-setting rate is high, the hybrid seed yield of hybrid paddy rice can be improved with its preparation ternary hybrid rice, reduce production cost and reduce environmental pollution.
Technical scheme is as follows:
1) select erect head rice varieties Shennong-265 (A), long stigma rice varieties sea B(B), long fringe neck rice varieties IR68902eB1(C) for objective trait provide parent, it is keep system for recurrent parent (R) that China produces the bigger excellence of popularizing area three at present, make hybridization A/B, F must be hybridized1In generation, with F1-ABRepresent;
2) plantation hybridization F1In generation, make hybridization F in florescence1-AB/ C, must reestablish diplomatic relations F1, with F1-ABCRepresent;
3) plantation F1-ABC, selfing sowing obtains F2-ABC, plant F2-ABC, colony's 1800-2000 strain, full heading time screening has the plant listing mark of erect head, long stigma and long fringe neck character simultaneously, and the period of maturation is individual plant sowing (F respectively3-ABC);
4) strain plantation 3) there is while gained the F of erect head, long stigma and long fringe neck character3-ABC, each strain plants 30 strains, and full heading time screening has erect head, long stigma and long fringe neck character and character without strain 2-3 separated simultaneously, therefrom selects individual plant 3 strain that economical character is excellent, with recurrent parent (R) for maternal, makes hybridization R/F3-ABC, F must be hybridized1In generation, with F1-ABCRRepresent;
5) plantation 4) gained F1-ABCR, period of maturation mixing sowing obtains F2-ABCR, plant F2-ABCR, colony's 1400-1600 strain, full heading time screening has the individual plant of erect head, long stigma and long fringe neck character simultaneously, therefrom selects individual plant 2 strain that economical character is excellent, and backcross R/F2-ABCR, must backcross F1In generation, with BC1F1Represent;
6) BC that backcrosses is planted1F1, selfing obtains BC1F2, plant BC1F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and individual plant 2 strain therefrom selecting economical character and recurrent parent R most like makes female parent with recurrent parent R() continue to backcross, must backcross BC2F1Generation;
7) BC that backcrosses is planted2F1In generation, selfing obtains BC2F2, plant BC2F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and individual plant 2 strain therefrom selecting economical character and recurrent parent R most like makes female parent with recurrent parent R() continue to backcross, must backcross BC3F1Generation;
8) step 7) is repeated until BC4F1In generation, plant BC4F1, selfing obtains BC4F2, plant BC4F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and the individual plant 1-2 strain sizing i.e. incubation long fringe neck three of erect head long stigma therefrom selecting economical character and recurrent parent R most like is keep system, is called for short ZCeB system;
9) ZCeB system is keep being corresponding for recurrent parent R CMS line (making female parent) 3 generations of hybridizing and backcross with three, is namely bred as the long fringe neck CMS line of erect head long stigma, is called for short ZCeA system;
The selection of the long fringe neck CMS line of erect head long stigma, its recurrent parent three is keep being mark 1B for Long Tepu B, flower 2B or Fujian, and its corresponding CMS line is that 1A is marked in Long Tepu A, flower 2A or Fujian.
Beneficial effects of the present invention:
Effect of the invention is that by hybridize and back cross breeding method control erect head, long stigma and long fringe neck character channel genes in the CMS line of hybrid rice, it is bred as the CMS line with erect head, long stigma and long fringe neck character, this sterile line not bag neck, stigma exposing ratio are high, outcrossing seed-setting rate is high, can improve the hybrid seed yield of hybrid paddy rice with its preparation ternary hybrid rice, reduce production cost and reduce environmental pollution. Seed farm every mu can increase yield 8-12%, reduces and sprays gibberellins cost 60-80 unit, increases economic benefit 150-220 unit.
Detailed description of the invention:
Embodiment 1:
1) select erect head rice varieties Shennong-265 (A), long stigma rice varieties sea B(B), long fringe neck rice varieties IR68902eB1(C) for objective trait provide parent, Long Tepu B recurrent parent (R), make hybridization A/B, F must be hybridized1In generation, with F1-ABRepresent;
2) plantation hybridization F1In generation, make hybridization F in florescence1-AB/ C, must reestablish diplomatic relations F1, with F1-ABCRepresent;
3) plantation F1-ABC, selfing sowing obtains F2-ABC, plant F2-ABC, colony 1800 strain, full heading time screening has the plant listing mark of erect head, long stigma and long fringe neck character simultaneously, and the period of maturation is individual plant sowing (F respectively3-ABC);
4) strain plantation 3) there is while gained the F of erect head, long stigma and long fringe neck character3-ABC, each strain plants 30 strains, and full heading time screening has erect head, long stigma and long fringe neck character and character without the strain 2 separated simultaneously, therefrom selects individual plant 3 strain that economical character is excellent, with recurrent parent (R) for maternal, makes hybridization R/F3-ABC, F must be hybridized1In generation, with F1-ABCRRepresent;
5) plantation 4) gained F1-ABCR, period of maturation mixing sowing obtains F2-ABCR, plant F2-ABCR, colony 1400 strain, full heading time screening has the individual plant of erect head, long stigma and long fringe neck character simultaneously, therefrom selects individual plant 2 strain that economical character is excellent, and backcross R/F2-ABCR, must backcross F1In generation, with BC1F1Represent;
6) BC that backcrosses is planted1F1, selfing obtains BC1F2, plant BC1F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and individual plant 2 strain therefrom selecting economical character and recurrent parent R most like makes female parent with recurrent parent R() continue to backcross, must backcross BC2F1Generation;
7) BC that backcrosses is planted2F1In generation, selfing obtains BC2F2, plant BC2F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and individual plant 2 strain therefrom selecting economical character and recurrent parent R most like makes female parent with recurrent parent R() continue to backcross, must backcross BC3F1Generation;
8) step 7) is repeated until BC4F1In generation, plant BC4F1, selfing obtains BC4F2, plant BC4F2Full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and the individual plant 1-2 strain sizing i.e. incubation long fringe neck three of erect head long stigma therefrom selecting economical character and recurrent parent R most like is keep system's (being called for short ZC Long Te Pu eB);
9) " ZC Long Te Pu eB " makes female parent with Long Tepu A() 3 generations of hybridizing and backcross, namely it is bred as the long fringe neck CMS line of erect head long stigma (being called for short ZC Long Te Pu eA);
" ZC Long Te Pu eA " has erect head, long stigma and long fringe neck character, " ZC Long Te Pu eA " not bag neck, stigma exposing ratio are high, outcrossing seed-setting rate is high, yield 8% can be increased with its preparation ternary hybrid rice seed farm every mu, reduce and spray gibberellins cost 60 yuan, increase economic benefit 150 yuan.
Embodiment 2:
1) select erect head rice varieties Shennong-265 (A), long stigma rice varieties sea B(B), long fringe neck rice varieties IR68902eB1(C) for objective trait provide parent, flower 2B be recurrent parent (R), make hybridization A/B, F must be hybridized1In generation, with F1-ABRepresent;
2) plantation hybridization F1In generation, make hybridization F in florescence1-AB/ C, must reestablish diplomatic relations F1, with F1-ABCRepresent;
3) plantation F1-ABC, selfing sowing obtains F2-ABC, plant F2-ABC, colony 1900 strain, full heading time screening has the plant listing mark of erect head, long stigma and long fringe neck character simultaneously, and the period of maturation is individual plant sowing (F respectively3-ABC);
4) strain plantation 3) there is while gained the F of erect head, long stigma and long fringe neck character3-ABC, each strain plants 30 strains, and full heading time screening has erect head, long stigma and long fringe neck character and character without the strain 3 separated simultaneously, therefrom selects individual plant 3 strain that economical character is excellent, with recurrent parent (R) for maternal, makes hybridization R/F3-ABC, F must be hybridized1In generation, with F1-ABCRRepresent;
5) plantation 4) gained F1-ABCR, period of maturation mixing sowing obtains F2-ABCR, plant F2-ABCR, colony 1500 strain, full heading time screening has the individual plant of erect head, long stigma and long fringe neck character simultaneously, therefrom selects individual plant 2 strain that economical character is excellent, and backcross R/F2-ABCR, must backcross F1In generation, with BC1F1Represent;
6) BC that backcrosses is planted1F1, selfing obtains BC1F2, plant BC1F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and individual plant 2 strain therefrom selecting economical character and recurrent parent R most like makes female parent with recurrent parent R() continue to backcross, must backcross BC2F1Generation;
7) BC that backcrosses is planted2F1In generation, selfing obtains BC2F2, plant BC2F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and individual plant 2 strain therefrom selecting economical character and recurrent parent R most like makes female parent with recurrent parent R() continue to backcross, must backcross BC3F1Generation;
8) step 7) is repeated until BC4F1In generation, plant BC4F1, selfing obtains BC4F2, plant BC4F2, full heading time screening has a plant of erect head, long stigma and long fringe neck character simultaneously, and the individual plant 1-2 strain sizing therefrom selecting economical character and recurrent parent R most like to be namely bred as the long fringe neck three of erect head long stigma be keep system's (be called for short ZC flower 2eB system);
9) " ZC flower 2eB " makes female parent with flower 2A() 3 generations of hybridizing and backcross, namely it is bred as the long fringe neck CMS line of erect head long stigma (being called for short ZC flower 2eA);
" ZC flower 2eA " has erect head, long stigma and long fringe neck character, " ZC flower 2eA " not bag neck, stigma exposing ratio are high, outcrossing seed-setting rate height, yield 10% can be increased with its preparation ternary hybrid rice seed farm every mu, reduce and spray gibberellins cost 70 yuan, increase economic benefit 190 yuan.
Embodiment 3:
1) select erect head rice varieties Shennong-265 (A), long stigma rice varieties sea B(B), long fringe neck rice varieties IR68902eB1(C) for objective trait provide parent, Fujian mark 1B be recurrent parent (R), make hybridization A/B, F must be hybridized1In generation, with F1-ABRepresent;
2) plantation hybridization F1In generation, make hybridization F in florescence1-AB/ C, must reestablish diplomatic relations F1, with F1-ABCRepresent;
3) plantation F1-ABC, selfing sowing obtains F2-ABC, plant F2-ABC, colony 2000 strain, full heading time screening has the plant listing mark of erect head, long stigma and long fringe neck character simultaneously, and the period of maturation is individual plant sowing (F respectively3-ABC);
4) strain plantation 3) there is while gained the F of erect head, long stigma and long fringe neck character3-ABC, each strain plants 30 strains, and full heading time screening has erect head, long stigma and long fringe neck character and character without the strain 3 separated simultaneously, therefrom selects individual plant 3 strain that economical character is excellent, with recurrent parent (R) for maternal, makes hybridization R/F3-ABC, F must be hybridized1In generation, with F1-ABCRRepresent;
5) plantation 4) gained F1-ABCR, period of maturation mixing sowing obtains F2-ABCR, plant F2-ABCR, colony 1600 strain, full heading time screening has the individual plant of erect head, long stigma and long fringe neck character simultaneously, therefrom selects individual plant 2 strain that economical character is excellent, and backcross R/F2-ABCR, must backcross F1In generation, with BC1F1Represent;
6) BC that backcrosses is planted1F1, selfing obtains BC1F2, plant BC1F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and individual plant 2 strain therefrom selecting economical character and recurrent parent R most like makes female parent with recurrent parent R() continue to backcross, must backcross BC2F1Generation;
7) BC that backcrosses is planted2F1In generation, selfing obtains BC2F2, plant BC2F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and individual plant 2 strain therefrom selecting economical character and recurrent parent R most like makes female parent with recurrent parent R() continue to backcross, must backcross BC3F1Generation;
8) step 7) is repeated until BC4F1In generation, plant BC4F1, selfing obtains BC4F2, plant BC4F2Full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and the individual plant 1-2 strain sizing i.e. incubation long fringe neck three of erect head long stigma therefrom selecting economical character and recurrent parent R most like is keep system's (being called for short ZC Fujian mark 1eB system);
9) " ZC Fujian mark 1eB " and Fujian mark 1A(make female parent) 3 generations of hybridizing and backcross, namely it is bred as the long fringe neck CMS line of erect head long stigma (being called for short ZC Fujian mark 1eA);
" ZC Fujian mark 1eA " has erect head, long stigma and long fringe neck character, " ZC Fujian mark 1eA " not bag neck, stigma exposing ratio are high, outcrossing seed-setting rate height, yield 12% can be increased with its preparation ternary hybrid rice seed farm every mu, reduce and spray gibberellins cost 80 yuan, increase economic benefit 220 yuan.
The foregoing is only presently preferred embodiments of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to the covering scope of the present invention.
Claims (1)
1. the selection of the long fringe neck CMS line of erect head long stigma, it is characterised in that: specifically comprising the following steps that of described selection
1) select erect head rice varieties Shennong-265 (A), long stigma rice varieties sea B(B), long fringe neck rice varieties IR68902eB1(C) for objective trait provide parent, it is keep system for recurrent parent (R) that China produces the bigger excellence of popularizing area three at present, make hybridization A/B, F must be hybridized1In generation, with F1-ABRepresent;
2) plantation hybridization F1In generation, make hybridization F in florescence1-AB/ C, must reestablish diplomatic relations F1, with F1-ABCRepresent;
3) plantation F1-ABC, selfing sowing obtains F2-ABC, plant F2-ABC, colony's 1800-2000 strain, full heading time screening has the plant listing mark of erect head, long stigma and long fringe neck character simultaneously, and period of maturation individual plant sowing respectively obtains F3-ABC;
4) plantation 3) there is while gained the F of erect head, long stigma and long fringe neck character3-ABC, each strain plants 30 strains, and full heading time screening has erect head, long stigma and long fringe neck character and character without strain 2-3 separated simultaneously, therefrom selects individual plant 3 strain that economical character is excellent, with recurrent parent (R) for maternal, makes hybridization R/F3-ABC, F must be hybridized1In generation, with F1-ABCRRepresent;
5) plantation 4) gained F1-ABCR, period of maturation mixing sowing obtains F2-ABCR, plant F2-ABCR, colony's 1400-1600 strain, full heading time screening has the individual plant of erect head, long stigma and long fringe neck character simultaneously, therefrom selects individual plant 2 strain that economical character is excellent, and backcross R/F2-ABCR, must backcross F1In generation, with BC1F1Represent;
6) BC that backcrosses is planted1F1, selfing obtains BC1F2, plant BC1F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and individual plant 2 strain therefrom selecting economical character and recurrent parent R most like continues to backcross as female parent with recurrent parent R, and must backcross BC2F1Generation;
7) BC that backcrosses is planted2F1In generation, selfing obtains BC2F2, plant BC2F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and individual plant 2 strain therefrom selecting economical character and recurrent parent R most like continues to backcross as female parent with recurrent parent R, and must backcross BC3F1Generation;
8) step 7) is repeated until BC4F1In generation, plant BC4F1, selfing obtains BC4F2, plant BC4F2, full heading time screening has the plant of erect head, long stigma and long fringe neck character simultaneously, and the individual plant 1-2 strain sizing i.e. incubation long fringe neck three of erect head long stigma therefrom selecting economical character and recurrent parent R most like is keep system, is called for short ZCeB system;
9) ZCeB system is keep the CMS line being corresponding for recurrent parent R to make hybridization of female parent 3 generations that backcrossed with three, is namely bred as the long fringe neck CMS line of erect head long stigma, is called for short ZCeA system;
Recurrent parent three is keep being mark 1B for Long Tepu B, flower 2B or Fujian, and its corresponding CMS line is that 1A is marked in Long Tepu A, flower 2A or Fujian.
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| CN104126497A (en) * | 2014-07-14 | 2014-11-05 | 福建农林大学 | Method for breeding high-stress-resistance giant embryo rice sterile line |
| CN104137764A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of three-line genic male sterile line of high-resistance glabrous yellow-leaf giant embryo rice |
| CN104137767A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of high-stress resistance elongated upper internode three-line genic male sterile line |
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| CN104126497A (en) * | 2014-07-14 | 2014-11-05 | 福建农林大学 | Method for breeding high-stress-resistance giant embryo rice sterile line |
| CN104137764A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of three-line genic male sterile line of high-resistance glabrous yellow-leaf giant embryo rice |
| CN104137767A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of high-stress resistance elongated upper internode three-line genic male sterile line |
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Effective date of registration: 20180509 Address after: 410200 Lei Feng North Road 106, Gaotang Ling Street, Wangcheng, Changsha, Hunan. Patentee after: Hunan Shennong Dafeng seed industry science and Technology Co., Ltd. Address before: 710016 B 1616, Tiandi Times Plaza, Fengcheng two road, Weiyang District, Xi'an, Shaanxi. Patentee before: Bie Chao |