CN105613257B - A kind of samsara selection of cotton sterile line - Google Patents
A kind of samsara selection of cotton sterile line Download PDFInfo
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- CN105613257B CN105613257B CN201511020758.2A CN201511020758A CN105613257B CN 105613257 B CN105613257 B CN 105613257B CN 201511020758 A CN201511020758 A CN 201511020758A CN 105613257 B CN105613257 B CN 105613257B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of samsara selection of cotton sterile line, recurrent selection is carried out based on bud Huang photoperiod-sensitive male sterile line, a samsara is formed by bud Huang photoperiod-sensitive male sterile line underlying group, hybridization group, separation restructuring group, stable selection four populations of group, passes through a wheel or more wheel selection and breeding sprouting Huang photoperiod-sensitive male sterile lines；Hybridize group, by cotton bud Huang photoperiod-sensitive male sterile line and multiple and different types, have objective trait, can normal natural propagation and cotton strain without sterile gene or cenospecies material hybridize based on establish；Material carries out recurrent selection based on present invention selection bud Huang photoperiod-sensitive male sterile line, easy to offspring's early stage separation identification；Bud Huang photoperiod-sensitive male sterile line carries out batch hybridization as bridge tool (platform or reactor), labor intensity can not only be reduced, save a large amount of recruitments, improve work efficiency, many different types of satisfactory sterile line breeding materials or resource can also be created, is a kind of innovative sterile line breeding method.
Description
Technical field
The present invention relates to cotton sterile line breeding field, more particularly to a kind of samsara selection of cotton sterile line.
Background technology
The male sterility line of plants (abbreviation sterile line) applied at present in production is divided into two major classes：CMS line, two systems
Sterile line (mainly includes recessive gms line and photosensitive recessive cytoblast sterile dual purpose lines).Three systems and the sterility of recessive gms line
Keep that manually fertility will be kept it to sterile line pollination with maintainer；The sterility of photosensitive recessive cytoblast sterile dual purpose lines is kept
Sowing time, which need to only be adjusted, to be fallen its florescence just to keep it from fertility in its pollen fertile period, be not required to manually participate in.But institute
It is all to utilize hybrid excellent in seed on the maternal pollination harvest female parent of sterile line with restorer pollen to have sterile line hybrid breeding
Gesture.
Non-Bt cotton sterile line breeding has male parent pedigree method and hybridization pollination to keep maternal sterility two parts content, male parent
Pedigree method is two target parents of selection (one or two parent contains the maternal sterile gene gene of holding) hybridization, then passes through side
Side is selected to survey (including sterility keeps test cross backcrossing and cenospecies fertility restorer test cross, to two systems or Breeding of Ternary) pedigree choosing
To select, final purification maintainer and then purifying sterile line, variety test of lines selection and breeding sterile line and new varieties are carried out using cenospecies,
Its advantage：Hybridization pedigree understands that genetic connection can be looked into, and the breeding time limit is shorter, easily goes out kind；Shortcoming：Parent's number is limited, loses
Biography background is simple, and hereditary basis is narrow, and offspring separates limited types, and sterile line keeps sterility and breeding to need artificial pollination, labor
Fatigue resistance is big, how of high cost process links are.
Recurrent selection is a kind of effective means of population improvement, its basic law, that is, genic balance law：At one completely
In the colony of panmixia, if (such as select, be mutated, genetic shift) interference without other factors, gene and gene
Type frequency constant is each constant from generation to generation.The essence of population improvement and crop breeding seeks to constantly break colony's gene and base
Because of the balance of type, the frequency of necessary for human gene and genotype in modified colony is constantly improved.From the perspective of breeding,
Selection and genetic recombination are the principal element and power of colony's gene and genotype frequency change.Improved by crop groups, can
To improve breeding efficiency and breeding level.
Bud Huang photoperiod-sensitive male sterile line is used primarily as first-filial generation maternal breeding at present, is directly used in hybrid seed
Production and breeding, the patent of invention of such as Yu Wenpu《A kind of breeding and its matched breeding method of cotton sterile line》(patent is awarded
Weigh notification number CN1040828C), fast patent of invention is set in such as analogy《The breeding side of the yellow mark photoperiod-sensitive male sterile line of one seed bud
Method》(license notification number CN102415332B).The present invention is used using it as genetic tool or resource.
The content of the invention
, can be with the technical problem to be solved in the present invention is to provide a kind of samsara selection of innovative cotton sterile line
Many different types of satisfactory sterile line germ plasm resources are obtained at the same time, sterility, process letter are kept without artificial pollination
List, labor intensity is low and work efficiency is high.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：
A kind of samsara selection of cotton sterile line, carries out samsara choosing based on cotton bud Huang photoperiod-sensitive male sterile line
Select, one is formed by cotton bud Huang photoperiod-sensitive male sterile line underlying group, hybridization group, separation restructuring group, stable selection four populations of group
A samsara, passes through a wheel or more wheel selection and breeding sprouting Huang photoperiod-sensitive male sterile lines；The hybridization group, with the quick male of cotton bud yellow light not
Educate is with multiple and different types, with objective trait, the normal natural propagation of energy and the cotton strain without sterile gene or hybridization
Kind material batch is established based on hybridizing.
Further, in the hybridization group, F is hybridized1Carry out nature selfing and/or hybridization F again for plant1For plant conduct
Male parent is returned with cotton bud Huang photoperiod-sensitive male sterile line.
Further, specifically comprise the following steps：(1) cotton bud Huang photoperiod-sensitive male sterile line establishes basis by being selfed
Group；(2) in cotton bud Huang photoperiod-sensitive male sterile line underlying group, selector is fated the sterile line of ground breeding objective, it is local not
Educate season and multiple and different types, have objective trait can normal natural propagation and the cotton strain or miscellaneous without sterile gene
Kind of a material hybridization is handed over, produces a collection of hybridization F1For seed；Hybridize F1Male parent and the quick male of cotton bud yellow light are done after being planted for seed not
It is backcrossing harvest backcrossing F to educate1For seed, meanwhile, hybridize F1Selfing harvests F naturally after being planted for seed2Seed, composition hybridization group；
(3) next enter separation restructuring group, F is returned with cotton bud Huang photoperiod-sensitive male sterile line1For seed, about 50% is obtained after plantation
The quick sterile plant of bud yellow light be used for recurrent selection breeding female parent, F1F is harvested on plant2Seed plants F again2Plant, about isolates
25% sterile plant and 75% fertile plant, two above source sterile plant are selfed homozygosis in its fertile phase, are further divided naturally
Fertile plant, the sterile plant of significant adverse character individual and diseased plant are removed during from, selection；(4) enter after 3-5 generation restructuring and stablize
Group is selected, sterile line colony or individual of the selection with objective trait carry out pedigree selection composition subgroup, then by screening, with survey
Test kind the test cross in selection, final choice objective trait and the outstanding sterile line of Comprehensive Traits.
By using above-mentioned technical proposal, the present invention has at least the following advantages：
(1) new germ plasm resource is created：Material carries out based on present invention selection cotton bud Huang photoperiod-sensitive male sterile line
Recurrent selection, it has bud yellow and photosensitive sterile two genes are close linkage heredity, and is all recessive inheritance character, just
In offspring's early stage separation identification.Cotton bud Huang photoperiod-sensitive male sterile line carries out miscellaneous in batches as bridge tool (platform or reactor)
Hand over, only the paternal pollen with objective trait need to be taken to pollinate, reduce labor intensity, save a large amount of recruitments, improve work effect
Rate obtains a collection of cross combination.Outstanding strain and sterile plant are selected in the cross combination offspring having outstanding performance again.New one
Wheel transformation improves sterile line.Many different types of satisfactory sterile lines can be created by the selection of the present invention to educate
Kind material or resource, are a kind of innovative sterile line breeding methods.
(2) the excellent kind of selection and breeding Comprehensive Traits：By recurrent selection improve improve colony, can select high yield, it is high-quality,
Precocious, more anti-sterile line, conventional variety and cenospecies extensively fitted.
(3) adaptability of Exotic Germplasm is improved：Pollinated by platform with Exotic Germplasm and improve the adaptability of adventive.
Brief description of the drawings
Above-mentioned is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention, below
With reference to attached drawing, the present invention is described in further detail with embodiment.
Fig. 1 is the cotton sterile line samsara selection flow chart of the present invention.
Embodiment
The cotton sterile line samsara selection of the present invention is taken turns based on cotton bud Huang photoperiod-sensitive male sterile line
Return selection.
Cotton bud Huang photoperiod-sensitive male sterile line is in the Anhui Province Anqing Wangjiang County nakamise the Changjiang river of middle and lower reach of Yangtze River ecology
Experiment station cotton normal growth season shows as：Stamen of blooming before June 20 all has certain loose powder ability, can be selfed naturally
Into bell but mote rate height, sow again in second year positive season and still show the quick sterile gene of bud yellow light；By the end of June to early September this when
Duan Kaihua stamens are showed without powder, that is, male sterility, and gynoecium can receive foreign pollen Pollination Fertilization Cheng Ling, for producing cenospecies；
Mid or late September stamen of blooming has loose powder ability, can be selfed into bell naturally but most seeds are not mature enough, second year positive season is broadcast again
Kind still shows photosensitive sterile gene.
Stamen has loose powder ability when Hainan winter is numerous then, and performance can be selfed into bell naturally but mote rate is higher, Nan Fan
After return to the local positive last month of spring and broadcast, it is i.e. sterile to early September performance by the end of June still to show two foregoing stages, for outcrossing
(hybridization), before June 20 or mid or late September is selfed and is used to keep kind of property and a breeding into bell naturally.
The sterile gene of cotton bud Huang photoperiod-sensitive male sterile line shows as the simple heredity of a pair of alleles control, F1
Plant is completely fertile, F2Plant law of segregation genotype is 1AA+2Aa+1aa, phenotypic segregation ratio example about 3：1.
As shown in Figure 1, the recurrent selection of cotton bud Huang photoperiod-sensitive male sterile line, by cotton bud Huang photoperiod-sensitive male sterile line base
Plinth group, hybridize group, separation restructuring group, stablize four populations one samsaras of composition of selection group, by a wheel or more wheel hybridization, divides
From, homozygous, pedigree identification selection and breeding sprouting Huang photoperiod-sensitive male sterile line.Different types of photoperiod-sensitive male sterile line is cultivated to be used to hybridize
Photoperiod-sensitive male sterile line is improved, or New Line Selected is as breeding material or applied to production.
Specifically comprise the following steps：
(1) cotton bud Huang photoperiod-sensitive male sterile line establishes underlying group by selfing；
(2) in cotton bud Huang photoperiod-sensitive male sterile line underlying group, selector be fated ground breeding objective sterile line, work as
Ground infertility season (July early and middle ten days to mid-August) with 15-20 it is different types of, have objective trait can normal natural propagation
And the cotton strain without sterile gene or the hybridization of cenospecies material, produce a collection of hybridization F1For seed；
F1Male parent (bud Huang heterozygosis F is after being planted for seed1) and cotton bud Huang photoperiod-sensitive male sterile line backcrossing harvest F1Generation kind
Son, while hybridize F1Selfing harvests F naturally after being planted for seed2Seed, composition hybridization group；
(3) next enter separation restructuring group, F is returned with cotton bud Huang photoperiod-sensitive male sterile line1For seed, obtained after plantation
The quick sterile plant of bud yellow light to about 50% is used for the female parent of recurrent selection breeding, and about 50% fertile plant is used for F2Seed hybrid
Plant；The bud Huang heterozygosis F that second year is harvested at the same time1F on plant2Seed plants F again2Plant, about isolate 25% sterile plant and
75% fertile plant, two above source sterile plant is homozygous in its fertile phase selfing, further separation, and when selection removes fertile
Strain, the sterile plant individual of significant adverse character and diseased plant；
(4) by 3-5 generation restructuring after enter stablize selection group, selection with objective trait sterile line colony or individual into
The row pedigree selection composition subgroup such as subgroup is for example precocious, high-quality, disease-resistant, then by screening, with Testers in selection test cross, finally
Selection target character and the outstanding sterile line of Comprehensive Traits.
Technical scheme is described in detail with concrete application example below：
First Year：0704204S is an original bud Huang photoperiod-sensitive male sterile line in 2007, and infertility is received at the beginning of 11 months then
Selfed seed in strain；
Second year：Continue within 2008 to plant this sterile material, early July to late August shows male sterility, during this period
Hybridization, which is carried out, with 18 different types of cotton varieties prepares F1Seed, harvests 18 combination seeds；Sterile line mid or late September is certainly
The male-sterile seed of right self propagated；
3rd year：The F for planting 18 combinations respectively in 20091Seed development is F1Plant, all shows male-fertile, appoints
Its free pollination harvests F2Seed；And use F1Plant pollen is returned with former sterile line 0704204S, harvests 18 backcrossing F1Seed；
36 combined hybrid seeds are harvested altogether；Sterile line nature self propagated male-sterile seed；
4th year：The F for planting 18 combinations respectively in 20102Seed, respectively grows into F2200 plants of plant, separates, respectively
Combination has the ratio that 38-45 plants of sterile plants account for 1/4, but economical character has difference, harvest nature selfing mid-November of marking
Seed, by the mixed basic material received sterile line 18, sterile line is improved as transformation of combination；Each combination fertile plant, removes obvious
Often combination is single respectively after weak plant, the plant that dies of illness receives, and every plant is received 2 wadding bell mixing, receives 18 combination F altogether3Seed makees selfing next year
Separate seed.
Plant 18 backcrossing combination F respectively at the same time1Seed, respectively grows into F1200 plants of plant, separates, and each combination has
85-96 plants of sterile plants account for 1/2 ratio, but economical character has difference, and harvest nature selfed seed mid-November of marking, is pressed
The mixed basic material received sterile line 18, sterile line is improved as transformation of combination；Each combination fertile plant, removes 2 backcrossing combinations,
List is received respectively for often combination after removing obvious weak plant, the plant that dies of illness, and every plant is received 2 wadding bell mixing, altogether 16 combination F of receipts2Seed is made
Selfing separation next year seed.
5th year：Continue within 2011 to plant 36 sterile lines, 18 F combined respectively3Seed and 16 backcrossing combination F2Kind
Son, it combines plant and mixes receipts male-sterile seed, F by subgroups such as ripe phase, plant height, disease resistances accordingly4Seed and backcrossing combination F3Kind
Son.Its sterile plant isolated is selected and remain according to fertility, economical character, and the basic material of sterile line is improved as transformation.
6th year：Positive season in 2012 plants the sterile line subgroup seed of last year respectively, selected in each subgroup it is excellent not
Single plant 50 is educated, in November to Hainan winter numerous identification, unconspicuous strain is separated by trait expressions such as plant height, florescence, knot bells
Row is mixed to be received, and receives 108 plant seeds altogether.
7th year：108 plant seed plantations are carried out test cross with Testers, harvested by positive season in 2013 into 108 strains
108 test cross kinds and 108 sterile lines.
8th year：The 108 test cross kinds and corresponding 108 sterile lines that plantation last year in 2014 harvests, with provinces and regions examination
The control as identification garden is compareed, meets 5 to set control, it is elected to investigate selection 50 according to time of infertility economical character, economic characters
Combination, and fine individual plant will be selected in the corresponding sterile line strain of elected combination, breeding is further purified November to Hainan in Winter
Into strain.Therefrom select 14170954S, 14170956S, 14170957S, 14170967S, 14180980S, 14180983S
Deng sterile line.Following table is test cross kind variety protection result in 2014.
The cotton infertility samsara selection of the present invention, uses the samsara based on cotton bud Huang photoperiod-sensitive male sterile line
System of selection, makes full use of the quick male sterility gene of cotton bud yellow light, closed inbreeding (Constantly allogamous) is changed into open
Hybridization, although hybridization pedigree is unknown, breeding cycle length.But save cotton sterile line manually to keep pollinating, process is simpler.Can be real
Existing polygenes restructuring, hybrid strain can be hundreds and thousands of.Segregating population hereditary basis is extensive.
The above described is only a preferred embodiment of the present invention, not make limitation in any form to the present invention, this
Field technology personnel make a little simple modification, equivalent variations or modification using the technology contents of the disclosure above, all fall within this hair
In bright protection domain.
Claims (1)
1. a kind of samsara selection of cotton sterile line, it is characterised in that based on cotton bud Huang photoperiod-sensitive male sterile line
Recurrent selection is carried out, by cotton bud Huang photoperiod-sensitive male sterile line underlying group, hybridization group, separation restructuring group, stable selection group four
Population forms a samsara, passes through a wheel or more wheel selection and breeding sprouting Huang photoperiod-sensitive male sterile lines；
It is described that group is recombinated by cotton bud Huang photoperiod-sensitive male sterile line underlying group, hybridization group, separation, stablizes selection Qun Sigezhong groups
Specifically comprise the following steps into a samsara：
(1) cotton bud Huang photoperiod-sensitive male sterile line establishes underlying group by selfing；
(2) in cotton bud Huang photoperiod-sensitive male sterile line underlying group, selector is fated the sterile line of ground breeding objective, it is local not
Educate season and multiple and different types, have objective trait can normal natural propagation and the cotton strain or miscellaneous without sterile gene
Kind of a material hybridization is handed over, produces a collection of hybridization F1For seed；
Hybridize F1Make male parent and cotton bud Huang photoperiod-sensitive male sterile line backcrossing harvest backcrossing F after being planted for seed1For seed, meanwhile,
Hybridize F1Selfing harvests F naturally after being planted for seed2Seed, composition hybridization group；
(3) next enter separation restructuring group, F is returned with cotton bud Huang photoperiod-sensitive male sterile line1For seed, bud is obtained after plantation
The quick sterile plant of yellow light is used for the female parent of recurrent selection breeding, hybridizes F1The F harvested on plant2Seed plants F again2Plant, isolates
Sterile plant and fertile plant, two above source sterile plant are selfed homozygosis naturally in its fertile phase, further separation, when selection remove
Fertile plant, the sterile plant of significant adverse character individual and diseased plant；
(4) enter after 3-5 generation restructuring and stablize selection group, sterile line colony or individual system of the selection with objective trait
Spectrum selection composition subgroup, then by screening, with Testers while selection while test cross, final choice objective trait and Comprehensive Traits are outstanding
Sterile line.
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| CN201511020758.2A CN105613257B (en) | 2015-12-31 | 2015-12-31 | A kind of samsara selection of cotton sterile line |
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| CN201511020758.2A CN105613257B (en) | 2015-12-31 | 2015-12-31 | A kind of samsara selection of cotton sterile line |
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| CN105613257B true CN105613257B (en) | 2018-05-15 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110122316B (en) * | 2018-02-09 | 2021-12-28 | 中国农业科学院棉花研究所 | Cotton photosensitive nuclear male sterile mutant and application thereof |
| CN109496837A (en) * | 2018-11-08 | 2019-03-22 | 江苏沿海地区农业科学研究所 | A kind of cotton variety circulation selection |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1535570A (en) * | 2003-04-04 | 2004-10-13 | 吉林省农业科学院水稻研究所 | Biserial random rotational selective seed-breeding method |
| CN102415332B (en) * | 2011-09-29 | 2013-08-28 | 中国农业科学院棉花研究所 | Breeding method of yellow bud marker photosensitive male sterile line |
| CN103598083B (en) * | 2013-09-11 | 2015-03-25 | 山西省农业科学院棉花研究所 | Method of synchronously breeding cotton restorer line and sterile line |
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