CN104531586A - Microbial agent for processing carcasses and preparation method and application thereof - Google Patents
Microbial agent for processing carcasses and preparation method and application thereof Download PDFInfo
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
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Abstract
The invention discloses a microbial agent for processing carcasses and a preparation method and application of the microbial agent. The microbial agent for processing the carcasses is formed by mixing five cultures according to a certain ratio. After a corresponding amount of microbial agent and the carcasses are mixed, at the temperature ranging from 70 DEG C to 90 DEG C, carcasses can be almost completely degraded within 24 hours and converted to usable microorganism organic fertilizer. Accordingly, the scientific purposes of rapid recycling, harmlessness and reduction of the carcasses on the site are achieved, and the microbial agent has a good application prospect.
Description
Technical field:
The invention belongs to carcase harmless treatment field, be specifically related to a kind of microbiobacterial agent processing carcase and its preparation method and application.
Background technology:
Livestock industry is fast-developing, dies of illness, the improper carcase died of illness is increasing, become the outstanding problem of the current Field of Animal Epidemic Disease Control of impact and sustainable development of animal husbandry.Harmless treatment is carried out to these carcases, be related to food safety, public health security, environmental safety and livestock industry sustainable, develop in a healthy way, be also the important process made conservation culture, build Urban Modern Agriculture and city of the world.Arbitrarily abandon carcase, there is potential pathophoresis harm, once the carcase of carrying infecting both domestic animals and human encephalapthy agent is discarded in refuse container, river course, Hu Bo even Residential areas, will the health of the serious harm mankind, even if the carcase of natural death, also can to environment.For production of developing animal husbandry, ensure that the people is healthy, implementing animals died of illness harmless treatment has become the current urgent task needing solution badly.How to make science, low-carbon (LC) and fast processing to dead livestock and poultry, be the key technical problem in innoxious and recycling treatment.
Existing innoxious and resource treatment technique respectively has relative merits, and according to investigations, the method that microorganism and plant and instrument combine is most widely used in present carcase process.And in this technique, the function of microorganism is carcase degradation speed and the key issue transforming valuable resource, thus development, develop the important ring that H.D microbiobacterial agent is resource utilization in carcase process.
Summary of the invention:
The object of the present invention is to provide one can fast degradation carcase, change into organic fertilizer, realize the microbiobacterial agent of process carcase of carcase quick resource, innoxious and minimizing process.
The microbiobacterial agent of process carcase of the present invention, it is characterized in that, described microbiobacterial agent is mixed according to mass ratio 1:0.5 ~ 1.5 by composite bacteria liquid and carrier, containing aspergillus oryzae (Asperqillus oryzae) CICC20142.0 ~ 3.0 × 10 in described composite bacteria liquid
12individual/L, thermophilus streptococcus (Streptococcus thermophilus) CICC 2011.0 ~ 3.0 × 10
13individual/L, yeast (Saccharomyces cerevisiae) CICC 10021.0 ~ 3.0 × 10
13individual/L, actinomycetes (Thermoactinomycessp) CICC 105233.0 ~ 3.5 × 10
12individual/L and bacillus licheniformis (Bacillus licheniformis) CICC 100921.0 ~ 2.5 × 10
13individual/L; Described carrier is mixed according to mass ratio 1:2.5 ~ 3.5 by Semen Maydis powder and rice bran.
Preferably, described microbiobacterial agent is mixed according to mass ratio 1:1 by composite bacteria liquid and carrier, and described carrier is mixed according to mass ratio 1:3 by Semen Maydis powder and rice bran.
Second object of the present invention is to provide a kind of preparation method processing the microbiobacterial agent of carcase, it is characterized in that, comprises the following steps:
A, cultivate aspergillus oryzae (Asperqillus oryzae) CICC 2014 respectively, thermophilus streptococcus (Streptococcusthermophilus) CICC 201, yeast (Saccharomyces cerevisiae) CICC 1002, actinomycetes (Thermoactinomyces sp) CICC 10523 and bacillus licheniformis (Bacillus licheniformis) CICC 10092, obtain its bacterium liquid, then by aspergillus oryzae (Asperqillus oryzae) CICC 2014, thermophilus streptococcus (Streptococcusthermophilus) CICC 201, yeast (Saccharomyces cerevisiae) CICC 1002, actinomycetes (Thermoactinomyces sp) CICC 10523 and bacillus licheniformis (Bacillus licheniformis) CICC 10092 bacterium liquid are mixed to form composite bacteria liquid, make in composite bacteria liquid containing aspergillus oryzae (Asperqillus oryzae) CICC 20142.0 ~ 3.0 × 10
12individual/L, thermophilus streptococcus (Streptococcus thermophilus) CICC 2011.0 ~ 3.0 × 10
13individual/L, yeast (Saccharomyces cerevisiae) CICC 10021.0 ~ 3.0 × 10
13individual/L, actinomycetes (Thermoactinomyces sp) CICC 105233.0 ~ 3.5 × 10
12individual/L and bacillus licheniformis (Bacillus licheniformis) CICC 100921.0 ~ 2.5 × 10
13individual/L,
B, Semen Maydis powder and rice bran are mixed formation carrier according to mass ratio 1:2.5 ~ 3.5;
C, composite bacteria liquid and carrier are mixed the microbiobacterial agent of formation processing carcase according to mass ratio 1:0.5 ~ 1.5.
Preferably, described microbiobacterial agent is mixed according to mass ratio 1:1 by composite bacteria liquid and carrier, and described carrier is mixed according to mass ratio 1:3 by Semen Maydis powder and rice bran.
3rd object of the present invention is to provide the application of microbiobacterial agent in degraded carcase of above-mentioned process carcase.
The microbiobacterial agent of process carcase of the present invention is according to necessarily mixing the rear complex micro organism fungicide formed than row by 5 strain bacterial classifications, after mixing with the carcase of rubbing according to corresponding amount, at the temperature of 70-90 DEG C, almost can degradable carcase in 24h, and be converted into available microorganism organic fertilizer.Thus original position solves the sciences problems of carcase quick resource, innoxious and minimizing, has extraordinary application prospect.
Embodiment:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
1, the preparation of aspergillus oryzae liquid
Aspergillus oryzae (Asperqillus oryzae CICC 2014) is chosen a ring from aspergillus oryzae slant medium to be seeded in liquid aspergillus oryzae seed culture medium, in 22 ~ 28 DEG C, static gas wave refrigerator 12 ~ 48h, when bacterial strain is in logarithmic phase, stop cultivating, obtained aspergillus oryzae first class inoculum; Then fill in the fermentor tank of aspergillus oryzae seed culture medium by the inoculum size access of 1 ~ 5%v/v, temperature is 22 ~ 28 DEG C, 24 ~ 72 hours static sealed fermenting time, obtain aspergillus oryzae (Asperqillus oryzae) CICC 2014 bacterium liquid, described aspergillus oryzae slant medium often rises containing wort 150mL, agar 20g, surplus is water, pH nature.Described aspergillus oryzae seed culture medium often rises containing wort 100-300ml, glucose 3 ~ 5g, and surplus is water, pH6.5 ~ 7.5.
Each substratum in embodiment is all that after each component being mixed, sterilizing is for subsequent use, lower same.
2, the preparation of streptococcus thermophilus fermentation liquid
The bacterium of described thermophilus streptococcus (Streptococcus thermophilus) CICC 201 is prepared by the following method: thermophilus streptococcus is chosen a ring from thermophilus streptococcus slant medium and is seeded in fluid milk bacillus seed culture medium, in 28 ~ 37 DEG C, quiescent culture 24 ~ 60h, when bacterial strain is in logarithmic phase, stop cultivating, obtained Bacterium lacticum first class inoculum, then fill in the fermentor tank of Bacterium lacticum seed culture medium by the inoculum size access of 1 ~ 5%v/v, temperature 35 ~ 40 DEG C, fermentation time 24 ~ 72 hours, obtain thermophilus streptococcus (Streptococcus thermophilus) CICC 201 bacterium liquid, described thermophilus streptococcus slant medium often rises containing skim-milk 100.0g, yeast powder 1g, K2HPO45g, agar 20g, surplus is water, pH is 6 ~ 7, described Bacterium lacticum seed culture medium often rises containing peptone 2 ~ 3g, glucose 2 ~ 3g, and surplus is water, pH6.0 ~ 7.0.
3, the preparation of S. cervisiae liquid
The bacterium of described yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC 1002 is prepared by the following method: yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC 1002 is chosen a ring from candidiasis slant medium and is seeded in liquid S. cervisiae seed culture medium, in 28 ~ 37 DEG C, 150 ~ 200rpm, shaking culture 12 ~ 48h, when bacterial strain is in logarithmic phase, stop cultivating, obtained S. cervisiae first class inoculum; Then fill in the fermentor tank of S. cervisiae seed culture medium by the inoculum size access of 1 ~ 5%v/v, temperature is 28 ~ 35 DEG C, mixing speed 200 ~ 300rpm, air flow 2 ~ 4L/ (Lmin), fermentation time 24 ~ 72 hours, obtain yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC 1002 bacterium liquid, described S. cervisiae slant medium often rises containing glucose 5g, peptone 2g, yeast extract paste 1g, sodium-chlor 0.5g, agar 20g, surplus is water, and pH is 6.5 ~ 7.5; Described S. cervisiae seed culture medium often rises containing peptone 3 ~ 4g, yeast extract paste 7 ~ 9g, glucose 3 ~ 5g, and surplus is water, pH6.5 ~ 7.5.
4, the preparation of actinomycetes bacterium liquid
The bacterium of described actinomycetes (Thermoactinomyces sp) CICC 10523 is prepared by the following method: actinomycetes (Thermoactinomyces sp) CICC 10523 is chosen a ring from candidiasis slant medium and is seeded in liquid actinomyces bacterium seed culture medium, in 28 ~ 37 DEG C, 150 ~ 200rpm, shaking culture 12 ~ 48h, when bacterial strain is in logarithmic phase, stop cultivating, obtained actinomyces bacterium first class inoculum; Then fill in the fermentor tank of actinomyces bacterium seed culture medium by the inoculum size access of 1 ~ 5%v/v, temperature is 28 ~ 35 DEG C, mixing speed 200 ~ 300rpm, air flow 2 ~ 4L/ (Lmin), fermentation time 24 ~ 72 hours, obtain actinomycetes (Thermoactinomyces sp) CICC 10523 bacterium liquid, described candidiasis slant medium often rises containing yeast powder 0.50g, peptone 0.50g, casein hydrolysate 0.50g, glucose 0.50g, Zulkovsky starch 0.50g, Sodium.alpha.-ketopropionate 0.30g, KH
2pO
40.30g, MgSO
47H
2o 0.05g, agar 15g, distilled water 1000mL, final pH 7.2; Described actinomyces bacterium seed culture medium often rises containing wort powder 3 ~ 4g, yeast extract paste 7 ~ 9g, and surplus is water, pH6.5 ~ 7.5.
5, the preparation of bacillus licheniformis bacterium liquid
Described Bacillus licheniformis (Bacillus licheniformis) CICC 10092 is prepared by the following method: Bacillus licheniformis (Bacillus licheniformis) CICC 10092 is chosen a ring from Bacillus licheniformis slant medium and is seeded in liquid Bacillus licheniformis seed culture medium, in 28 ~ 37 DEG C, 150 ~ 230rpm, shaking culture 24 ~ 48h, when bacterial strain is in logarithmic phase, stop cultivating, obtained Bacillus licheniformis first class inoculum, then fill in the fermentor tank of Bacillus licheniformis seed culture medium by the inoculum size access of 1 ~ 5%v/v, temperature 28 ~ 35 DEG C, mixing speed 250 ~ 350rpm, air flow 1 ~ 2L/ (Lmin), fermentation time 24 ~ 72 hours, obtain Bacillus licheniformis (Bacillus licheniformis) CICC10092 bacterium liquid, described Bacillus licheniformis slant medium often rises containing peptone 9 ~ 11g, extractum carnis 4.5 ~ 5.5g, glucose 9 ~ 11g, sodium-chlor 4.5 ~ 5.5g, agar 20g, surplus is water, pH is 6.5 ~ 7.5, described Bacillus licheniformis seed culture medium often rises containing peptone 4 ~ 5g, yeast extract paste 2 ~ 3g, glucose 3 ~ 5g, surplus is water, pH7.0 ~ 8.5.
Embodiment 1:
Above-mentioned aspergillus oryzae (Asperqillus oryzae) CICC 2014 bacterium liquid, thermophilus streptococcus (Streptococcusthermophilus) CICC 201 bacterium liquid, yeast (Saccharomyces cerevisiae) CICC 1002 bacterium liquid, actinomycetes (Thermoactinomyces sp) CICC 10523 bacterium liquid and bacillus licheniformis (Bacillus licheniformis) CICC 10092 bacterium liquid are mixed to form composite bacteria liquid, make in composite bacteria liquid containing aspergillus oryzae (Asperqillus oryzae) CICC 20142.0 × 10
12individual/L, thermophilus streptococcus (Streptococcus thermophilus) CICC 2011.0 × 10
13individual/L, yeast (Saccharomycescerevisiae) CICC 10021.0 × 10
13individual/L, actinomycetes (Thermoactinomyces sp) CICC 105233.0 × 10
12individual/L and bacillus licheniformis (Bacillus licheniformis) CICC 100921.0 × 10
13individual/L.
Semen Maydis powder and rice bran are mixed obtained carrier according to the ratio of mass ratio 1:3.5.
Composite bacteria liquid and carrier are mixed according to mass ratio 1:1.5, obtains the microbiobacterial agent processing carcase.
In 600kg carcase, add the microbiobacterial agent of the process carcase of 1.2kg, mix, at being placed at 70 DEG C, after 24h, remaining carcase is 80kg, decrease 520kg, reduction rate is 520/600*100%=90%, and carcase is converted into available microorganism organic fertilizer.Thus original position solves the sciences problems of carcase quick resource, innoxious and minimizing.
Embodiment 2:
Above-mentioned aspergillus oryzae (Asperqillus oryzae) CICC 2014 bacterium liquid, thermophilus streptococcus (Streptococcusthermophilus) CICC 201 bacterium liquid, yeast (Saccharomyces cerevisiae) CICC 1002 bacterium liquid, actinomycetes (Thermoactinomyces sp) CICC 10523 bacterium liquid and bacillus licheniformis (Bacillus licheniformis) CICC 10092 bacterium liquid are mixed to form composite bacteria liquid, make in composite bacteria liquid containing aspergillus oryzae (Asperqillus oryzae) CICC 20143.0 × 10
12individual/L, thermophilus streptococcus (Streptococcus thermophilus) CICC 2013.0 × 10
13individual/L, yeast (Saccharomycescerevisiae) CICC 10023.0 × 10
13individual/L, actinomycetes (Thermoactinomyces sp) CICC 105233.5 × 10
12individual/L and bacillus licheniformis (Bacillus licheniformis) CICC 100922.5 × 10
13individual/L;
Semen Maydis powder and rice bran are mixed obtained carrier according to the ratio of mass ratio 1:2.5.
Composite bacteria liquid and carrier are mixed according to mass ratio 1:0.5, obtains the microbiobacterial agent processing carcase.
The microbiobacterial agent of the process carcase of 1.67kg is added in 835kg carcase, mix, at being placed at 90 DEG C, after 24h, remaining carcase is 120kg, decrease 715kg, reduction rate is 715/835*100%=85.63%, and carcase is converted into available microorganism organic fertilizer.Thus original position solves the sciences problems of carcase quick resource, innoxious and minimizing.
Embodiment 3:
Aspergillus oryzae (Asperqillus oryzae) CICC 2014 bacterium liquid, thermophilus streptococcus (Streptococcus thermophilus) CICC 201 bacterium liquid, yeast (Saccharomyces cerevisiae) CICC 1002 bacterium liquid, actinomycetes (Thermoactinomycessp) CICC 10523 bacterium liquid and bacillus licheniformis (Bacillus licheniformis) CICC 10092 bacterium liquid are mixed to form composite bacteria liquid, make in composite bacteria liquid containing aspergillus oryzae (Asperqillus oryzae) CICC 20142.5 × 10
12individual/L, thermophilus streptococcus (Streptococcus thermophilus) CICC 2012 × 10
13individual/L, yeast (Saccharomyces cerevisiae) CICC 10022 × 10
13individual/L, actinomycetes (Thermoactinomyces sp) CICC 105233.2 × 10
12individual/L and bacillus licheniformis (Bacillus licheniformis) CICC 100922 × 10
13individual/L;
Semen Maydis powder and rice bran are mixed obtained carrier according to the ratio of mass ratio 1:3.
Composite bacteria liquid and carrier are mixed according to mass ratio 1:1, obtains the microbiobacterial agent processing carcase.
The microbiobacterial agent of the process carcase of 1.446kg is added in 723kg carcase, mix, at being placed at 80 DEG C, after 24h, remaining carcase is 48kg, decrease 675kg, reduction rate is 675/723*100%=93.36%, and carcase is converted into available microorganism organic fertilizer.Thus original position solves the sciences problems of carcase quick resource, innoxious and minimizing.
Embodiment 4:
Aspergillus oryzae (Asperqillus oryzae) CICC 2014 bacterium liquid, thermophilus streptococcus (Streptococcus thermophilus) CICC 201 bacterium liquid, yeast (Saccharomyces cerevisiae) CICC 1002 bacterium liquid, actinomycetes (Thermoactinomycessp) CICC 10523 bacterium liquid and bacillus licheniformis (Bacillus licheniformis) CICC 10092 bacterium liquid are mixed to form composite bacteria liquid, make in composite bacteria liquid containing aspergillus oryzae (Asperqillus oryzae) CICC 20142.6 × 10
12individual/L, thermophilus streptococcus (Streptococcus thermophilus) CICC 2012.2 × 10
13individual/L, yeast (Saccharomyces cerevisiae) CICC 10021.8 × 10
13individual/L, actinomycetes (Thermoactinomyces sp) CICC 105233.3 × 10
12individual/L and bacillus licheniformis (Bacillus licheniformis) CICC 100922.1 × 10
13individual/L;
Semen Maydis powder and rice bran are mixed obtained carrier according to the ratio of mass ratio 1:2.7.
Composite bacteria liquid and carrier are mixed according to mass ratio 1:0.6, obtains the microbiobacterial agent processing carcase.
The microbiobacterial agent of the process carcase of 1.816kg is added in 908kg carcase, mix, at being placed at 80 DEG C, after 24h, remaining carcase is 122kg, decrease 786kg, reduction rate is 786/908*100%=86.56%, and carcase is converted into available microorganism organic fertilizer.Thus original position solves the sciences problems of carcase quick resource, innoxious and minimizing.
Claims (7)
1. one kind processes the microbiobacterial agent of carcase, it is characterized in that, described microbiobacterial agent is mixed according to mass ratio 1:0.5 ~ 1.5 by composite bacteria liquid and carrier, containing aspergillus oryzae (Asperqillus oryzae) CICC2014 2.0 ~ 3.0 × 10 in described composite bacteria liquid
12individual/L, thermophilus streptococcus (Streptococcus thermophilus) CICC 201 1.0 ~ 3.0 × 10
13individual/L, yeast (Saccharomyces cerevisiae) CICC 1,002 1.0 ~ 3.0 × 10
13individual/L, actinomycetes (Thermoactinomyces sp) CICC 10,523 3.0 ~ 3.5 × 10
12individual/L and bacillus licheniformis (Bacilluslicheniformis) CICC 10,092 1.0 ~ 2.5 × 10
13individual/L; Described carrier is mixed according to mass ratio 1:2.5 ~ 3.5 by Semen Maydis powder and rice bran.
2. the microbiobacterial agent of process carcase according to claim 1, it is characterized in that, described microbiobacterial agent is mixed according to mass ratio 1:1 by composite bacteria liquid and carrier.
3. the microbiobacterial agent of process carcase according to claim 1, it is characterized in that, described carrier is mixed according to mass ratio 1:3 by Semen Maydis powder and rice bran.
4. process a preparation method for the microbiobacterial agent of carcase, it is characterized in that, comprise the following steps:
A, cultivate aspergillus oryzae (Asperqillus oryzae) CICC 2014 respectively, thermophilus streptococcus (Streptococcusthermophilus) CICC 201, yeast (Saccharomyces cerevisiae) CICC 1002, actinomycetes (Thermoactinomyces sp) CICC 10523 and bacillus licheniformis (Bacillus licheniformis) CICC 10092, obtain its bacterium liquid, then by aspergillus oryzae (Asperqillus oryzae) CICC 2014, thermophilus streptococcus (Streptococcusthermophilus) CICC 201, yeast (Saccharomyces cerevisiae) CICC 1002, actinomycetes (Thermoactinomyces sp) CICC 10523 and bacillus licheniformis (Bacillus licheniformis) CICC 10092 bacterium liquid are mixed to form composite bacteria liquid, make in composite bacteria liquid containing aspergillus oryzae (Asperqillus oryzae) CICC 20142.0 ~ 3.0 × 10
12individual/L, thermophilus streptococcus (Streptococcus thermophilus) CICC 201 1.0 ~ 3.0 × 10
13individual/L, yeast (Saccharomyces cerevisiae) CICC 1,002 1.0 ~ 3.0 × 10
13individual/L, actinomycetes (Thermoactinomyces sp) CICC 10,523 3.0 ~ 3.5 × 10
12individual/L and bacillus licheniformis (Bacillus licheniformis) CICC 10,092 1.0 ~ 2.5 × 10
13individual/L,
B, Semen Maydis powder and rice bran are mixed formation carrier according to mass ratio 1:2.5 ~ 3.5;
C, composite bacteria liquid and carrier are mixed the microbiobacterial agent of formation processing carcase according to mass ratio 1:0.5 ~ 1.5.
5. preparation method according to claim 4, is characterized in that, described microbiobacterial agent is mixed according to mass ratio 1:1 by composite bacteria liquid and carrier.
6. preparation method according to claim 4, is characterized in that, described carrier is mixed according to mass ratio 1:3 by Semen Maydis powder and rice bran.
7. the application of microbiobacterial agent in degraded carcase of the carcase described in claim 1-3.
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| CN104894007A (en) * | 2015-05-12 | 2015-09-09 | 安徽农业大学 | Compound microorganism preparation, preparing method and application of compound microorganism preparation to processing animal carcasses |
| CN105062932A (en) * | 2015-09-09 | 2015-11-18 | 肇庆大华农生物药品有限公司 | Microbial flora preparation applied to harmless dead poultry body disposal and preparation method and application thereof |
| CN105199980A (en) * | 2015-09-10 | 2015-12-30 | 山东华牧天元农牧股份有限公司 | Compounded microbial preparation and method for innocent treatment of animal carcasses |
| CN105215043A (en) * | 2015-10-09 | 2016-01-06 | 南京元凯生物能源环保工程有限公司 | A kind of biodegradation fermentation bed and application thereof |
| CN105861386A (en) * | 2016-05-25 | 2016-08-17 | 广西南宁市圣农科技开发有限公司 | Biological zymocyte for treating pigs dying of diseases and preparation method of biological fermentation zymocyte |
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| CN107912609A (en) * | 2017-11-15 | 2018-04-17 | 湖南耶啰耶兔业发展有限公司 | A kind of biology aquaculture fertilizer and preparation method thereof |
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| CN110272851A (en) * | 2019-07-18 | 2019-09-24 | 江苏黑钻生物科技有限公司 | Microorganism formulation and its application |
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