CN1371767A - Fermentation agent for urban organic refuse biological treatment - Google Patents
Fermentation agent for urban organic refuse biological treatment Download PDFInfo
- Publication number
- CN1371767A CN1371767A CN 01106252 CN01106252A CN1371767A CN 1371767 A CN1371767 A CN 1371767A CN 01106252 CN01106252 CN 01106252 CN 01106252 A CN01106252 A CN 01106252A CN 1371767 A CN1371767 A CN 1371767A
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- CN
- China
- Prior art keywords
- biological treatment
- organic refuse
- fermentation
- fermentation agent
- urban organic
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The biological treatment fermentation agent for organic refuse is a powder preparation made of mixed (by weight) plant lactobacillus 1-3, thermophilic streptococcus 2-6, tropical candidin yeast 1-2 and viridin 1-5. Said invention can effectively degrade domestic organic refuse, and can convert it into useful resource, tis fermentation period can be shortened by 15-20 days, and the protein content in its product can be insreased by 2-5 times.
Description
The present invention relates to microorganism application technology, specifically a kind of organic waste biological treatment leavening.
Along with the development of producing, the increasing and the urban population high concentration of population, the quantity of municipal refuse constantly increases, contaminated environment day by day seriously, the Health and Living of giving the public is with great influence.Each city always transports a large amount of rubbish to rural area as organic fertilizer, owing to contain a large amount of materials such as brick and tile fragment in the rubbish, so chronic administration in the farmland, can progressively be destroyed soil aggregate and physicochemical property.In recent years, agricultural sector has refused to use rubbish in the farmland.Inoculum commonly used is human and animal excreta, mud of fermentation and composition thereof in the fermentation of present organic waste, and not only inoculation quality does not quantize, the effect instability, and fermentation period is long, and there are problems in contaminated environment etc.
The purpose of this invention is to provide a kind of bacteria leaven and replace fermentation process such as ight soil, it is stable to reach ferment effect, and fermentation period is short, fermentation agent for urban organic refuse biological treatment free from environmental pollution.
The present invention is by weight and gets Lactobacillus plantarum (Lactobacillus plantarum, AS1.557) 1～3; Streptococcus thermophilus (Streptococcus thermphilus, AS1.1855) 2～6; Candida tropicalis (Candidatropicalis, AS296) 1～2; Trichoderma viride (Trichoderma sp., AS3.4265) 1～5, four kinds of bacterium mix makes pulvis (above each bacterial classification is all purchased in Beijing Institute of Micro-biology).
Culture medium of the present invention
Slant medium
Lactobacillus plantarum culture medium: dusty yeast 1%, glucose 1.5%, calcium carbonate 1.5%, agar 2%, pH7.0,30-35 ℃
Improvement IRIE culture medium, tryptone 0.5, yeast extract 0.25, lactose 1, sodium succinate 1, KH
2PO
40.2, K
2HPO
40.2, agar 1.5, pH6.8 ± 0.2,37 ℃.
Czapek's medium is used to cultivate mould and saccharomycete: sucrose 30, K
2HPO
41, MgSO
40.5, NaNO
33, KCL 0.5, FeSO
40.01, water 1000ml, pH6.7.
Seed culture medium
Dusty yeast 0.5～1%, glucose 1～2%, tryptone 0.5～1%, NaCl 0.2～0.5%, pH6.0～8.0.
Loading amount: 5～10ml/30ml test tube, 20～50ml/250ml triangular flask
Fermentation medium
Dusty yeast 0.25～1%, glucose 0.5～1%, peptone 0.1～0.5%, beef extract 0.1～0.5%.Soluble starch 0.1～0.5%, NaCl 0.1～0.3%, Na
2HPO
40.1～0.5%, KH
2PO
40.01～0.1%, pH6.0～8.0.
Loading amount: 50ml/250ml triangular flask
Fermentation process and microbial inoculum technological process
Single strain bacterium liquid and preparation method thereof
Slant activation 16-24h---seed liquor 5ml/ test tube, 25--38 ℃, 100～160rpm cultivates 10-24h, inoculum concentration 3---10%; 25---38 ℃ of seed liquor 30---50ml/250ml triangular flask, 100---160rpm cultivates 10-30h, inoculum concentration 3---10%;
The fermenting agent technological process
Slant activation 16---24hr---seed liquor 2.5---5ml/ test tube, 25---38 ℃, 100---160rpm, cultivate 10-24hr, meet 3--10%,---30 ℃ of seed liquor 20ml/250ml triangular flasks, 10 ℃--160rpm, cultivate 10-24hr, inoculum concentration 1--10%,---zymotic fluid 100--200ml/500ml triangular flask, 30 ℃, 100---160rpm,------1--15% inoculum concentration---is to solid medium: mix after the inoculation, adorn 20--35 ℃ of koji tray, static cultivation 30-48h to cultivate 20-50hr., dry or the airing pulverizing for 20-45 ℃, various bacterium powder contain the bacterium number and reach 10
5---10
9CFU/g, the solid culture with 4 kinds of bacterium cooperates in proportion, adds suitable sodium chloride, urea etc. and mixes, and uses as leavening.
The present invention is directed to municipal domestic organic refuse, adopt high-efficiency strain powdery leavening to replace the method for natural inoculum fermentation such as ight soil, mud, coordinative role by microbe symbiotic principle and metabolite is effectively degraded domestic organic garbage, make it innoxious, and further be converted into available resources.Its fermentation period shortens 15～20 days, and the crude protein content in the product increases by 2～5 times.
Embodiment
Respectively with 4 kinds of microorganisms at slant activation 17hr, under 26 ℃, 110rpm condition, cultivate 11hr then, make seed liquor 5ml/ examination respectively, respectively be seeded in fermentation medium and (mix 22 ℃ of dress koji traies after the inoculation by 3%, static cultivation 30h is dried or the airing pulverizing for 25 ℃); 1. by weight with 1: 2: 1: 1 ratio cooperates the solid culture of 4 kinds of bacterium, adds suitable sodium chloride, urea etc. and mixes, and uses as leavening.2. by weight with 3: 6: 2: 5 ratio cooperates the solid culture of 4 kinds of bacterium, adds suitable sodium chloride, urea etc. and mixes, and uses as leavening.
1. 100 gram organic wastes are by this leavening of use 3%, and through fermentation in 5～7 days, crude protein content can reach 6.38% in the tunning, and crude fiber content is 0.7%.
2. 100 gram organic wastes are by this leavening of use 2.5%, and through fermentation in 6～8 days, crude protein content can reach 6.26% in the tunning, and crude fiber content is 0.8%.
Comparative example
Compare with the organic waste before the fermentation, crude protein content increases more than 2 times, and the crude fibre degradation rate is 41%.
And not using the fermentation starting of this rubbish fermentation agent method fermentation slow, fermentation period reaches 30 days, even longer.
Claims (2)
1. a fermentation agent for urban organic refuse biological treatment is characterized in that getting by weight Lactobacillus plantarum (Lactobacillus plantarum, AS 1.557) 1～3; Streptococcus thermophilus (Streptococcus thermphilus, AS1.1855) 2～6; Candida tropicalis (Candida tropicalis, AS296) 1～2; Trichoderma viride (Trichoderma sp., AS3.4265) 1～5, four kinds of bacterium mix makes pulvis.
2. fermentation agent for urban organic refuse biological treatment bacterium liquid and preparation method thereof is characterized in that slant activation 16--24h---seed liquor 5ml/ test tube, and 25--38 ℃, 100～160rpm cultivates 10-24h, inoculum concentration 3---10%; 25---38 ℃ of seed liquor 30---50ml/250ml triangular flask, 100---160rpm cultivates 10-30h, inoculum concentration 3---10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01106252 CN1371767A (en) | 2001-02-28 | 2001-02-28 | Fermentation agent for urban organic refuse biological treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01106252 CN1371767A (en) | 2001-02-28 | 2001-02-28 | Fermentation agent for urban organic refuse biological treatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1371767A true CN1371767A (en) | 2002-10-02 |
Family
ID=4655289
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01106252 Pending CN1371767A (en) | 2001-02-28 | 2001-02-28 | Fermentation agent for urban organic refuse biological treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1371767A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531586A (en) * | 2014-12-31 | 2015-04-22 | 广州舒国生物科技有限公司 | Microbial agent for processing carcasses and preparation method and application thereof |
| CN108373385A (en) * | 2018-04-24 | 2018-08-07 | 贵州贵谷农业股份有限公司 | A kind of preparation method of Rice Cropping dedicated fertilizer |
| CN108424183A (en) * | 2018-04-24 | 2018-08-21 | 贵州梵净山生态农业股份有限公司 | A kind of organic fertilizer fermentation active bacteria formulation |
| CN108424321A (en) * | 2018-04-24 | 2018-08-21 | 贵州贵谷农业股份有限公司 | A kind of Rice Cropping dedicated fertilizer |
| CN108530164A (en) * | 2018-04-24 | 2018-09-14 | 贵州贵谷农业股份有限公司 | A kind of high-yield rice implantation methods |
-
2001
- 2001-02-28 CN CN 01106252 patent/CN1371767A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531586A (en) * | 2014-12-31 | 2015-04-22 | 广州舒国生物科技有限公司 | Microbial agent for processing carcasses and preparation method and application thereof |
| CN108373385A (en) * | 2018-04-24 | 2018-08-07 | 贵州贵谷农业股份有限公司 | A kind of preparation method of Rice Cropping dedicated fertilizer |
| CN108424183A (en) * | 2018-04-24 | 2018-08-21 | 贵州梵净山生态农业股份有限公司 | A kind of organic fertilizer fermentation active bacteria formulation |
| CN108424321A (en) * | 2018-04-24 | 2018-08-21 | 贵州贵谷农业股份有限公司 | A kind of Rice Cropping dedicated fertilizer |
| CN108530164A (en) * | 2018-04-24 | 2018-09-14 | 贵州贵谷农业股份有限公司 | A kind of high-yield rice implantation methods |
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Legal Events
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |