CN105462879A - Preparation method of agricultural complex bacterium - Google Patents
Preparation method of agricultural complex bacterium Download PDFInfo
- Publication number
- CN105462879A CN105462879A CN201510915443.8A CN201510915443A CN105462879A CN 105462879 A CN105462879 A CN 105462879A CN 201510915443 A CN201510915443 A CN 201510915443A CN 105462879 A CN105462879 A CN 105462879A
- Authority
- CN
- China
- Prior art keywords
- preparation
- agricultural
- composite bacteria
- solid
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a preparation method of an agricultural complex bacterium. Agricultural microbial bacteria are selected according to purposes and the relative proportions of various strains are determined, and then the strains, as a complex strain, are applied to liquid and solid combined fermenting culture. The preparation method disclosed by the invention, through the solid and liquid combined culture on the agricultural complex microbial bacterium, can save cost and improve efficiency; various farming byproducts can be utilized reasonably, so that waste materials are recycled; in early stage, the various strains are rapidly expanded through liquid culture, and in later stage, the various microbial bacteria are cultured in a same fermentation tank, so that the strains are co-cultured and mutually coordinated, and co-survive in a same environment; therefore, the disintegrating degree among the strains is improved; and the preparation not only can improve the quantity of effective live bacteria but also can enhance the function of the complex bacterium.
Description
Technical field
The present invention relates to the cultivation field of composite bacteria, particularly relate to a kind of preparation method of agricultural composite bacteria.
Background technology
Current domestic microbiological microbial inoculum development rapidly, in order to do the function yeast of composite fertilizer, fertilizer, punching fertilising etc., effectively can improve utilization rate of fertilizer, and control bacterial fungus disease infection, instant effect, income is high.A lot of agriculture microbial composite bacteria used is mainly composite in varing proportions according to each bacterial classification using function now, or the bacterial classification of multiple same alike result is made into composite bacteria, separately through liquid culture or separately through solid culture, contradict each other between so easy generation bacterial classification, cause being difficult to practical function unification, effect is lower.
Summary of the invention
The technical problem to be solved in the present invention, is the preparation method providing a kind of agricultural composite bacteria, comprises the following steps:
1, choose bacterial classification: choose agricultural microorganism bacterium according to purposes and as composite bacteria after determining the relative proportion of each bacterial classification, described agricultural microorganism bacterium comprise in vinelandii, root nodule bacterium, genus bacillus, yeast, milk-acid bacteria, actinomycetes or fungi more than one;
2, liquid fermenting: by each agricultural microorganism bacterium of choosing according to respective culture medium prescription, by liquid fermenting, enlarged culturing obtains each microbial inoculum;
3, solid fermentation: drop into 60% Crop Residues, 20% soyflour or Semen Maydis powder, 10% molasses water solution and 10% composite bacteria liquid in solid-state fermentation tank successively, the solid fermentation of 48-72 hour is carried out after having fed intake, described composite bacteria liquid be step 2 by each microbial inoculum mixing gained after liquid fermenting, wherein the ratio of each bacterium liquid is the determined ratio of step 1;
4, discharging: after solid fermentation, discharging after detecting, the material moisture of discharging controls between 25-35%;
5, pack: tank under material, immediately pack and vacuumize.
Further, in described composite bacteria, the ratio of each bacterial classification is: vinelandii 30%-40%, root nodule bacterium 20%-30%, genus bacillus 30%-60%, yeast 20%-40%, milk-acid bacteria 20%-30%, actinomycetes 10%-20%, fungi 5%-10%.
Further, described Crop Residues is rice bran, the grain of rice or defatted rice bran.
Further, in described molasses water solution, molasses and water ratio are 1:3.
Further, in described step 3, in solid-state fermentation tank, each state modulator is: temperature is between 30-40 DEG C, and initial water sub-control is built in 30%-50%, and air quantity ratio controls between 0.2-0.5, and solid mixing speed controls at 2-5 rev/min.
Further, in described step 3, during solid fermentation, detected the parameter index of the moisture of material, pH value of water solution, OD600 value, enumeration every 4 hours.
Further, in described step 1, the ratio of choosing is that 40% genus bacillus, 40% yeast, 20% milk-acid bacteria are as the composite bacteria for livestock and poultry cultivation.
Further, in described step 2, genus bacillus adopts subtilis, its culture medium prescription: glucose 2%, starch 0.5%, yeast extract paste 1%, peptone 1%, potassium primary phosphate 0.2g%, dipotassium hydrogen phosphate 0.2%, sodium-chlor 0.5%, manganous sulfate 0.1%; PH value modulation 6.80-7.2, culture temperature controls at 31 DEG C, ferments 48 hours;
Described yeast adopts pichia spp, its culture medium prescription: glucose 2%, sucrose 1%, peptone 1%, yeast extract paste 1%, potassium primary phosphate 0.25%, dipotassium hydrogen phosphate 0.25%; PH nature, culture temperature controls at 29 DEG C, ferments 48 hours;
Described milk-acid bacteria adopts Lactobacterium acidophilum, its culture medium prescription: glucose 4%, sucrose 2%, peptone 1%, potassium primary phosphate 0.25%, dipotassium hydrogen phosphate 0.25%, corn steep liquor 1%, manganous sulfate 0.1%, magnesium sulfate 0.1%; PH controls between 6.5-6.8, culture temperature control 34 DEG C, fermentation 48 hours.
Further, in described step 3,60% rice bran and 20% soyflour is dropped in solid-state fermentation tank, through 130 DEG C, 0.1Mpa steam high-voltage sterilizing 30min, be cooled to after room temperature until it, then drop into 10% molasses water, be 121 DEG C and pressure through excess temperature be the high-temperature sterilization of 0.1Mpa, sterilizing continues 20-30min, is cooled to room temperature, then drops into 10% composite bacteria liquid again.
Further, in described step 3, after having fed intake, setting culture temperature in solid-state fermentation tank controls at 36 DEG C, initial water sub-control is built in 35%, and mixing control is at 4 revs/min, and first 24 hours stuffy, start after 24 hours to ventilate, ventilating ratio controls 0.35, starts logical hot blast after 60 hours, and temperature is set in 120 DEG C, when material moisture is down to 30%, stop logical hot blast and discharging; Under material, tank carries out vacuum packaging, living bacteria count >=1 wherein × 109cfu/g.
Further, in described step 1, choose vinelandii, root nodule bacterium, actinomycetes, bacillus megaterium as the composite bacteria for agricultural planting.
Further, in described step 1, choose Bacillus licheniformis, milk-acid bacteria, yeast, actinomycetes, fungi are composite bacteria for agricultural wastes biological compost.
The present invention has following advantage:
1, with the composite microbial bacteria of solid-liquid Combined culture agricultural use, cost-saving, raise the efficiency;
2, by different Crop Residues Appropriate application, can be turned waste into wealth;
3, by liquid culture, each bacterial classification is expanded rapidly early stage, different microbial bacterias is placed on same fermentor cultivation by the later stage, bacterial classification co-cultivation and mutual survival and mutually collaboratively to do under equivalent environment, improve the compatible degree between bacterial classification, not only improve living bacteria count and strengthen composite bacteria function simultaneously.
Embodiment
The preparation method of agricultural composite bacteria of the present invention, comprises the following steps:
1, bacterial classification is chosen: choose agricultural microorganism bacterium according to purposes and as composite bacteria after determining the ratio of each bacterial classification, in described composite bacteria, the ratio of each bacterial classification is: vinelandii 30%-40%, root nodule bacterium 20%-30%, genus bacillus 30%-60%, yeast 20%-40%, milk-acid bacteria 20%-30%, actinomycetes 10%-20%, fungi 5%-10%.
2, liquid fermenting: by each agricultural microorganism bacterium of choosing according to respective culture medium prescription, by liquid fermenting, enlarged culturing obtains each microbial inoculum;
3, solid fermentation: drop into 60% Crop Residues, 20% soyflour or Semen Maydis powder, 10% molasses water solution and 10% composite bacteria liquid in solid-state fermentation tank, the solid fermentation of 48-72 hour is carried out after having fed intake, described Crop Residues is rice bran, the grain of rice or defatted rice bran, in described molasses water solution, molasses and water ratio are 1:3, described composite bacteria liquid be step 2 by each microbial inoculum mixing gained after liquid fermenting, wherein the ratio of each bacterium liquid is the determined ratio of step 1; In solid-state fermentation tank, each state modulator is: temperature is between 30-40 DEG C, and initial water sub-control is built in 30%-50%, and air quantity ratio controls between 0.2-0.5, and solid mixing speed controls at 2-5 rev/min; Detected the parameter index of the moisture of material, pH value of water solution, OD600 value, enumeration every 4 hours during solid fermentation;
4, discharging: after solid fermentation, discharging after detecting, the material moisture of discharging controls between 25-35%;
5, pack: tank under material, immediately pack and vacuumize.
Embodiment 1
1, ratio of choosing is that 40% genus bacillus, 40% yeast, 20% milk-acid bacteria are as the composite bacteria for livestock and poultry cultivation.
2, by each agricultural microorganism bacterium of choosing according to respective culture medium prescription, by liquid fermenting, enlarged culturing obtains each microbial inoculum;
Described genus bacillus adopts subtilis, its culture medium prescription: glucose 2%, starch 0.5%, yeast extract paste 1%, peptone 1%, potassium primary phosphate 0.2g%, dipotassium hydrogen phosphate 0.2%, sodium-chlor 0.5%, manganous sulfate 0.1%; PH value modulation 6.80-7.2, culture temperature controls at 31 DEG C, ferments 48 hours;
Described yeast adopts pichia spp, its culture medium prescription: glucose 2%, sucrose 1%, peptone 1%, yeast extract paste 1%, potassium primary phosphate 0.25%, dipotassium hydrogen phosphate 0.25%; PH nature, culture temperature controls at 29 DEG C, ferments 48 hours;
Described milk-acid bacteria adopts Lactobacterium acidophilum, its culture medium prescription: glucose 4%, sucrose 2%, peptone 1%, potassium primary phosphate 0.25%, dipotassium hydrogen phosphate 0.25%, corn steep liquor 1%, manganous sulfate 0.1%, magnesium sulfate 0.1%; PH controls between 6.5-6.8, culture temperature control 34 DEG C, fermentation 48 hours.
3, in solid-state fermentation tank, drop into 60% rice bran and 20% soyflour, through 130 DEG C, 0.1Mpa steam high-voltage sterilizing 30min, be cooled to after room temperature until it, drop into 10% molasses water again, be 121 DEG C and pressure through excess temperature be the high-temperature sterilization of 0.1Mpa, sterilizing continues 20-30min, is cooled to room temperature, then drops into 10% composite bacteria liquid again;
After having fed intake, setting culture temperature in solid-state fermentation tank controls at 36 DEG C, initial water sub-control is built in 35%, and mixing control is at 4 revs/min, and first 24 hours stuffy, start after 24 hours to ventilate, ventilating ratio controls 0.35, starts logical hot blast after 60 hours, and temperature is set in 120 DEG C, when material moisture is down to 30%, stop logical hot blast.
4, tank under detection → discharging → material.
5, vacuum packaging → finished product, living bacteria count >=1 wherein × 109cfu/g.
Embody rule: the composite bacteria that above-mentioned production is good be applied on raising pigs, test piglet after 7 days from wean, piglet quantity is 30, and weight in average is 7kg, and the test period is 42 days.
Table 1 process of the test contrasts
Table 2 comparison of test results
As can be seen from data and the result of table 1 and table 2, use the piggy of biological compound fungi, not only dead quantity is few, also has obvious appearance investigation simultaneously, simultaneously than control group weightening finish 15.56%.Other economic benefit of later stage quality-improving also having meat etc.
Embodiment 2
Step 1, chooses vinelandii, root nodule bacterium, actinomycetes, bacillus megaterium as the composite bacteria for agricultural planting, other preparation process reference examples 1.
Embodiment 3
Step 1, chooses Bacillus licheniformis, milk-acid bacteria, yeast, actinomycetes, fungi is composite bacteria for agricultural wastes biological compost, other preparation process reference examples 1.
Embodiment 1 is most preferred embodiment of the present invention.
The present invention has following advantage:
1, with the composite microbial bacteria of solid-liquid Combined culture agricultural use, cost-saving, raise the efficiency;
2, by different Crop Residues Appropriate application, can be turned waste into wealth;
3, by liquid culture, each bacterial classification is expanded rapidly early stage, different microbial bacterias is placed on same fermentor cultivation by the later stage, bacterial classification co-cultivation and mutual survival and mutually collaboratively to do under equivalent environment, improve the compatible degree between bacterial classification, not only improve living bacteria count and strengthen composite bacteria function simultaneously.
Above to invention has been exemplary description; obvious specific implementation of the present invention is not subject to the restrictions described above; as long as have employed the improvement of the various unsubstantialities that method of the present invention is conceived and technical scheme is carried out; or design of the present invention and technical scheme directly applied to other occasion, all within protection scope of the present invention without to improve.
Claims (10)
1. a preparation method for agricultural composite bacteria, is characterized in that, comprises the following steps:
1, choose bacterial classification: choose agricultural microorganism bacterium according to purposes and as composite bacteria after determining the relative proportion of each bacterial classification, described agricultural microorganism bacterium comprise in vinelandii, root nodule bacterium, genus bacillus, yeast, milk-acid bacteria, actinomycetes or fungi more than one;
2, liquid fermenting: by each agricultural microorganism bacterium of choosing according to respective culture medium prescription, by liquid fermenting, enlarged culturing obtains each microbial inoculum;
3, solid fermentation: drop into 60% Crop Residues, 20% soyflour or Semen Maydis powder, 10% molasses water solution and 10% composite bacteria liquid in solid-state fermentation tank successively, the solid fermentation of 48-72 hour is carried out after having fed intake, described composite bacteria liquid be step 2 by each microbial inoculum mixing gained after liquid fermenting, wherein the ratio of each bacterium liquid is the determined ratio of step 1;
4, discharging: after solid fermentation, discharging after detecting, the material moisture of discharging controls between 25-35%;
5, pack: tank under material, immediately pack and vacuumize.
2. the preparation method of agricultural composite bacteria according to claim 1, it is characterized in that, in described composite bacteria, the ratio of each bacterial classification is: vinelandii 30%-40%, root nodule bacterium 20%-30%, genus bacillus 30%-60%, yeast 20%-40%, milk-acid bacteria 20%-30%, actinomycetes 10%-20%, fungi 5%-10%.
3. the preparation method of the preparation method of agricultural composite bacteria according to claim 1 and 2, it is characterized in that, in described step 3, in solid-state fermentation tank, each state modulator is: temperature is between 30-40 DEG C, initial water sub-control is built in 30%-50%, air quantity ratio controls between 0.2-0.5, and solid mixing speed controls at 2-5 rev/min.
4. the preparation method of the preparation method of agricultural composite bacteria according to claim 1 and 2, is characterized in that, in described step 3, detects the parameter index of the moisture of material, pH value of water solution, OD600 value, enumeration during solid fermentation every 4 hours.
5. the preparation method of the preparation method of agricultural composite bacteria according to claim 1, is characterized in that, in described step 1, the ratio of choosing is that 40% genus bacillus, 40% yeast, 20% milk-acid bacteria are as the composite bacteria for livestock and poultry cultivation.
6. the preparation method of the preparation method of agricultural composite bacteria according to claim 5, it is characterized in that, in described step 2, genus bacillus adopts subtilis, its culture medium prescription: glucose 2%, starch 0.5%, yeast extract paste 1%, peptone 1%, potassium primary phosphate 0.2g%, dipotassium hydrogen phosphate 0.2%, sodium-chlor 0.5%, manganous sulfate 0.1%; PH value modulation 6.80-7.2, culture temperature controls at 31 DEG C, ferments 48 hours;
Described yeast adopts pichia spp, its culture medium prescription: glucose 2%, sucrose 1%, peptone 1%, yeast extract paste 1%, potassium primary phosphate 0.25%, dipotassium hydrogen phosphate 0.25%; PH nature, culture temperature controls at 29 DEG C, ferments 48 hours;
Described milk-acid bacteria adopts Lactobacterium acidophilum, its culture medium prescription: glucose 4%, sucrose 2%, peptone 1%, potassium primary phosphate 0.25%, dipotassium hydrogen phosphate 0.25%, corn steep liquor 1%, manganous sulfate 0.1%, magnesium sulfate 0.1%; PH controls between 6.5-6.8, culture temperature control 34 DEG C, fermentation 48 hours.
7. the preparation method of the preparation method of agricultural composite bacteria according to claim 5, it is characterized in that, in described step 3, in solid-state fermentation tank, drop into 60% rice bran and 20% soyflour, through 130 DEG C, 0.1Mpa steam high-voltage sterilizing 30min, be cooled to after room temperature until it, dropping into 10% molasses water again, be 121 DEG C and pressure is the high-temperature sterilization of 0.1Mpa through excess temperature, and sterilizing continues 20-30min, be cooled to room temperature, then drop into 10% composite bacteria liquid again.
8. the preparation method of the preparation method of agricultural composite bacteria according to claim 1, is characterized in that, in described step 1, chooses vinelandii, root nodule bacterium, actinomycetes, bacillus megaterium as the composite bacteria for agricultural planting.
9. the preparation method of the preparation method of agricultural composite bacteria according to claim 1, is characterized in that, in described step 1, chooses Bacillus licheniformis, milk-acid bacteria, yeast, actinomycetes, fungi are composite bacteria for agricultural wastes biological compost.
10. the preparation method of the agricultural composite bacteria according to claim 1 or 2 or 5 or 6 or 7, it is characterized in that, in described molasses water solution, molasses and water ratio are 1:3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510915443.8A CN105462879A (en) | 2015-12-10 | 2015-12-10 | Preparation method of agricultural complex bacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510915443.8A CN105462879A (en) | 2015-12-10 | 2015-12-10 | Preparation method of agricultural complex bacterium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105462879A true CN105462879A (en) | 2016-04-06 |
Family
ID=55601041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510915443.8A Pending CN105462879A (en) | 2015-12-10 | 2015-12-10 | Preparation method of agricultural complex bacterium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105462879A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085922A (en) * | 2016-08-12 | 2016-11-09 | 中山市润泽生物科技有限公司 | Small-sized microbial ecosystem method prepares multifunctional composite microbe microbial inoculum and application |
| CN106748341A (en) * | 2017-03-23 | 2017-05-31 | 安徽莱姆佳生物科技股份有限公司 | Potato microbial bacterial manure special and preparation method thereof |
| CN107568249A (en) * | 2017-07-19 | 2018-01-12 | 北京百丰天下生物科技有限公司 | Bioprotective applied to prevention and cure of viruses and preparation method thereof |
| CN107619797A (en) * | 2016-07-21 | 2018-01-23 | 济南航晨生物科技有限公司 | One kind is based on solid waste mixed fermentation composite bacteria agent and its production technology |
| CN107723254A (en) * | 2016-08-10 | 2018-02-23 | 湖南山河美生物环保科技股份有限公司 | A kind of biological organic fertilizer zymophyte for discarded object fermentation process |
| CN108148779A (en) * | 2018-01-16 | 2018-06-12 | 天津生机集团股份有限公司 | A kind of organic matter decomposing inoculant and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1275551A (en) * | 2000-06-19 | 2000-12-06 | 韩承民 | Environmental protection biological bacterial manure and production method thereof |
| CN1568747A (en) * | 2004-04-27 | 2005-01-26 | 广州市希普生物饲料有限公司 | Direct feeding viable bacteria preparation and method for preparing it |
| CN1703968A (en) * | 2004-05-25 | 2005-12-07 | 中国农业大学 | Synbiotic feed addictive of mini-peptide with beneficial microbe and its usage |
| CN102987114A (en) * | 2012-10-08 | 2013-03-27 | 安徽长风农牧科技有限公司 | Feed additive for live pig breeding |
-
2015
- 2015-12-10 CN CN201510915443.8A patent/CN105462879A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1275551A (en) * | 2000-06-19 | 2000-12-06 | 韩承民 | Environmental protection biological bacterial manure and production method thereof |
| CN1568747A (en) * | 2004-04-27 | 2005-01-26 | 广州市希普生物饲料有限公司 | Direct feeding viable bacteria preparation and method for preparing it |
| CN1703968A (en) * | 2004-05-25 | 2005-12-07 | 中国农业大学 | Synbiotic feed addictive of mini-peptide with beneficial microbe and its usage |
| CN102987114A (en) * | 2012-10-08 | 2013-03-27 | 安徽长风农牧科技有限公司 | Feed additive for live pig breeding |
Non-Patent Citations (3)
| Title |
|---|
| 王贺祥: "《农业微生物学》", 31 August 2003 * |
| 董永胜: "《有机固体废弃物的处理及应用技术研究》", 30 September 2015 * |
| 陈谦等: "生物有机肥中几种功能微生物的研究及应用概况", 《应用与环境生物学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619797A (en) * | 2016-07-21 | 2018-01-23 | 济南航晨生物科技有限公司 | One kind is based on solid waste mixed fermentation composite bacteria agent and its production technology |
| CN107723254A (en) * | 2016-08-10 | 2018-02-23 | 湖南山河美生物环保科技股份有限公司 | A kind of biological organic fertilizer zymophyte for discarded object fermentation process |
| CN106085922A (en) * | 2016-08-12 | 2016-11-09 | 中山市润泽生物科技有限公司 | Small-sized microbial ecosystem method prepares multifunctional composite microbe microbial inoculum and application |
| CN106748341A (en) * | 2017-03-23 | 2017-05-31 | 安徽莱姆佳生物科技股份有限公司 | Potato microbial bacterial manure special and preparation method thereof |
| CN107568249A (en) * | 2017-07-19 | 2018-01-12 | 北京百丰天下生物科技有限公司 | Bioprotective applied to prevention and cure of viruses and preparation method thereof |
| CN108148779A (en) * | 2018-01-16 | 2018-06-12 | 天津生机集团股份有限公司 | A kind of organic matter decomposing inoculant and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105462879A (en) | Preparation method of agricultural complex bacterium | |
| CN102160595B (en) | Preparation process of complex microorganism fermented active feed | |
| CN105418172B (en) | The preparation method of biological organic fertilizer | |
| CN104293694B (en) | A kind of preparation method of sludge aerobic compost composite bacteria agent | |
| CN102132762B (en) | Straw feed and preparation method thereof | |
| CN103642726B (en) | A kind of maize straw organism of fermentation microbial inoculum and preparation method thereof and fermentation process | |
| CN104478515B (en) | Zymotic fluid, pleurotus eryngii culture medium and preparation method thereof for cultivating pleurotus eryngii matrix | |
| CN105462880A (en) | Preparation method of sewage processing complex bacterium | |
| CN102286413B (en) | Preparation method of liquid fermentation medium for bacillus thuringiensis | |
| CN104987157A (en) | Ganoderma lucidum antler culture medium utilizing domestic garbage and method for cultivating ganoderma lucidum antler | |
| CN101914445A (en) | Indigenous probiotic microorganism solid fungicide and preparation method and application thereof | |
| CN103409382B (en) | A kind of strengthen the technology of lignin degradation in Phanerochaete chrysosporium solid fermentation | |
| CN101851121B (en) | Compound bacterial agent for efficiently converting pig excrements and preparation method and application thereof | |
| CN103289937A (en) | Method for producing bacillus laterosporus live bacteria by high density solid fermentation | |
| CN103289935A (en) | Compound strain microecological preparation and preparation method thereof | |
| CN109497266A (en) | A kind of method that multi-cultur es composite fermentation produces high-quality biological feedstuff | |
| CN104509683A (en) | Method for preparing yellow corn silage feed by compounding and fermenting shells of bamboo shoots | |
| CN104509688A (en) | Method of producing biological feed by fermenting banana stem and leaf through micro-organism | |
| CN102731175A (en) | Microbial agent for composting organic material | |
| CN102334589B (en) | Method for preparing fermented feed from waste bacillus fermentation liquor | |
| CN103409383A (en) | Method used for accelerating lignin degradation in Aspergillus oryzae solid state fermentation | |
| CN103445020A (en) | Fermented feed for feeding pigs | |
| CN102940145A (en) | Preparation method of fermentation aquatic health care feed | |
| CN103205388B (en) | Method for producing viable bacteria of bacillus licheniformis by high-density solid fermentation | |
| CN108795812A (en) | A kind of compost decomposing agent and the preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160406 |
|
| WD01 | Invention patent application deemed withdrawn after publication |