CN104515760B - 双色荧光定位超分辨率生物显微方法及*** - Google Patents
双色荧光定位超分辨率生物显微方法及*** Download PDFInfo
- Publication number
- CN104515760B CN104515760B CN201410787361.5A CN201410787361A CN104515760B CN 104515760 B CN104515760 B CN 104515760B CN 201410787361 A CN201410787361 A CN 201410787361A CN 104515760 B CN104515760 B CN 104515760B
- Authority
- CN
- China
- Prior art keywords
- fluorescence
- image
- super
- resolution
- biological sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 50
- 239000012472 biological sample Substances 0.000 claims abstract description 94
- 230000004397 blinking Effects 0.000 claims abstract description 85
- 238000003384 imaging method Methods 0.000 claims abstract description 77
- 239000007853 buffer solution Substances 0.000 claims abstract description 50
- 230000008569 process Effects 0.000 claims abstract description 17
- 230000005284 excitation Effects 0.000 claims description 70
- 239000000523 sample Substances 0.000 claims description 36
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 32
- 230000004913 activation Effects 0.000 claims description 31
- 229940123973 Oxygen scavenger Drugs 0.000 claims description 29
- PZBFGYYEXUXCOF-UHFFFAOYSA-N TCEP Chemical compound OC(=O)CCP(CCC(O)=O)CCC(O)=O PZBFGYYEXUXCOF-UHFFFAOYSA-N 0.000 claims description 27
- 230000003287 optical effect Effects 0.000 claims description 20
- 102000016938 Catalase Human genes 0.000 claims description 18
- 108010053835 Catalase Proteins 0.000 claims description 18
- 235000010323 ascorbic acid Nutrition 0.000 claims description 16
- 229960005070 ascorbic acid Drugs 0.000 claims description 16
- 239000011668 ascorbic acid Substances 0.000 claims description 16
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 16
- 238000010276 construction Methods 0.000 claims description 14
- -1 oxygen scavenger Chemical compound 0.000 claims description 12
- FIKAKWIAUPDISJ-UHFFFAOYSA-L paraquat dichloride Chemical compound [Cl-].[Cl-].C1=C[N+](C)=CC=C1C1=CC=[N+](C)C=C1 FIKAKWIAUPDISJ-UHFFFAOYSA-L 0.000 claims description 12
- 108010015776 Glucose oxidase Proteins 0.000 claims description 11
- 235000019420 glucose oxidase Nutrition 0.000 claims description 11
- 239000004366 Glucose oxidase Substances 0.000 claims description 10
- 229940116332 glucose oxidase Drugs 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 10
- 230000005012 migration Effects 0.000 claims description 9
- 238000013508 migration Methods 0.000 claims description 9
- 108010001816 pyranose oxidase Proteins 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 6
- 230000001678 irradiating effect Effects 0.000 claims description 4
- 238000004364 calculation method Methods 0.000 claims description 3
- KDUIUFJBNGTBMD-VXMYFEMYSA-N cyclooctatetraene Chemical compound C1=C\C=C/C=C\C=C1 KDUIUFJBNGTBMD-VXMYFEMYSA-N 0.000 claims 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 20
- KDUIUFJBNGTBMD-DLMDZQPMSA-N [8]annulene Chemical compound C/1=C/C=C\C=C/C=C\1 KDUIUFJBNGTBMD-DLMDZQPMSA-N 0.000 description 19
- 210000004027 cell Anatomy 0.000 description 15
- 239000000203 mixture Substances 0.000 description 13
- 150000003573 thiols Chemical class 0.000 description 13
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 230000004807 localization Effects 0.000 description 5
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 4
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 4
- 230000003213 activating effect Effects 0.000 description 4
- 229930014669 anthocyanidin Natural products 0.000 description 4
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 4
- 235000008758 anthocyanidins Nutrition 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 238000000386 microscopy Methods 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 230000000886 photobiology Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 125000000143 2-carboxyethyl group Chemical group [H]OC(=O)C([H])([H])C([H])([H])* 0.000 description 2
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 description 2
- 101710128063 Carbohydrate oxidase Proteins 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000004061 bleaching Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003638 chemical reducing agent Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 150000004306 cyclooctatetraenes Chemical class 0.000 description 2
- 238000006392 deoxygenation reaction Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 150000004880 oxines Chemical class 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 150000003003 phosphines Chemical class 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- JLEXUIVKURIPFI-UHFFFAOYSA-N tris phosphate Chemical compound OP(O)(O)=O.OCC(N)(CO)CO JLEXUIVKURIPFI-UHFFFAOYSA-N 0.000 description 2
- LLTDOAPVRPZLCM-UHFFFAOYSA-O 4-(7,8,8,16,16,17-hexamethyl-4,20-disulfo-2-oxa-18-aza-6-azoniapentacyclo[11.7.0.03,11.05,9.015,19]icosa-1(20),3,5,9,11,13,15(19)-heptaen-12-yl)benzoic acid Chemical compound CC1(C)C(C)NC(C(=C2OC3=C(C=4C(C(C(C)[NH+]=4)(C)C)=CC3=3)S(O)(=O)=O)S(O)(=O)=O)=C1C=C2C=3C1=CC=C(C(O)=O)C=C1 LLTDOAPVRPZLCM-UHFFFAOYSA-O 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010021033 Hypomenorrhoea Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
- 230000002186 photoactivation Effects 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical group [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- 238000000492 total internal reflection fluorescence microscopy Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06113—Coherent sources; lasers
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Abstract
Description
Claims (13)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410787361.5A CN104515760B (zh) | 2014-12-17 | 2014-12-17 | 双色荧光定位超分辨率生物显微方法及*** |
US15/303,461 US10151697B2 (en) | 2014-12-17 | 2015-09-07 | Two-color fluorescence localization super-resolution biological microscopy method and system |
PCT/CN2015/089047 WO2016095572A1 (zh) | 2014-12-17 | 2015-09-07 | 双色荧光定位超分辨率生物显微方法及*** |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410787361.5A CN104515760B (zh) | 2014-12-17 | 2014-12-17 | 双色荧光定位超分辨率生物显微方法及*** |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104515760A CN104515760A (zh) | 2015-04-15 |
CN104515760B true CN104515760B (zh) | 2017-10-31 |
Family
ID=52791384
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410787361.5A Active CN104515760B (zh) | 2014-12-17 | 2014-12-17 | 双色荧光定位超分辨率生物显微方法及*** |
Country Status (3)
Country | Link |
---|---|
US (1) | US10151697B2 (zh) |
CN (1) | CN104515760B (zh) |
WO (1) | WO2016095572A1 (zh) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3298449B1 (en) * | 2015-05-22 | 2022-05-04 | The Hong Kong University of Science and Technology | Methods and systems for generating non-diffracting light sheets for multicolor fluorescence microscopy |
CN108693151A (zh) * | 2017-04-11 | 2018-10-23 | 中国科学技术大学 | Storm/palm显微成像方法及装置 |
DE102017131249A1 (de) * | 2017-12-22 | 2019-06-27 | Leica Microsystems Cms Gmbh | Verfahren zum Abbilden einer Probe unter Verwendung eines Fluoreszenzmikroskops mit stimulierter Emissionsdepletion |
CN108181282B (zh) * | 2018-01-03 | 2019-03-15 | 宁波纳美致生物科技有限公司 | 一种三通道荧光定位超分辨率生物显微***及方法 |
WO2020051764A1 (zh) * | 2018-09-11 | 2020-03-19 | 深圳大学 | 分子定位超分辨成像的降噪方法、装置及终端设备 |
CN109325914B (zh) * | 2018-09-11 | 2023-04-18 | 深圳大学 | 分子定位超分辨成像的降噪方法、装置及终端设备 |
CN111077298A (zh) * | 2019-12-21 | 2020-04-28 | 东南大学 | 一种免疫荧光共定位成像平台的制备方法及应用 |
CN111678902A (zh) * | 2020-07-24 | 2020-09-18 | 纳观生物有限公司 | 三通道荧光定位超分辨率生物显微***及显微方法 |
CN115753717A (zh) * | 2022-11-24 | 2023-03-07 | 深圳大学 | 一种单波长激发的荧光调制多色超分辨显微成像方法 |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102566048A (zh) * | 2012-01-17 | 2012-07-11 | 浙江大学 | 一种基于象散的样品轴向漂移补偿方法和装置 |
CN102589428A (zh) * | 2012-01-17 | 2012-07-18 | 浙江大学 | 基于非对称入射的样品轴向位置跟踪校正的方法和装置 |
EP2535755A1 (en) * | 2011-06-14 | 2012-12-19 | Ecole Polytechnique Fédérale de Lausanne (EPFL) | Cumulant microscopy |
CN103399413A (zh) * | 2013-07-30 | 2013-11-20 | 浙江大学 | 基于双螺旋光束的样品轴向漂移检测及补偿方法和装置 |
CN103575716A (zh) * | 2013-11-15 | 2014-02-12 | 中国科学院长春应用化学研究所 | 磁场调控的超分辨荧光成像方法 |
CN103592278A (zh) * | 2013-11-21 | 2014-02-19 | 中国计量学院 | 基于荧光发射抑制机理的随机定位超分辨显微方法及装置 |
CN104089935A (zh) * | 2014-07-11 | 2014-10-08 | 中国科学院化学研究所 | 超分辨荧光寿命相关光谱*** |
CN104122662A (zh) * | 2014-08-15 | 2014-10-29 | 北京大学 | 一种超高密度超分辨光学闪烁显微成像***及方法 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2399169A1 (en) * | 2001-09-07 | 2003-03-07 | Queen's University At Kingston | Diagnostic methods for determining susceptibility to convulsive conditions |
US7700375B2 (en) * | 2004-06-16 | 2010-04-20 | Valorisation-Recherche, Lp | Fluorescent labeling of specific protein targets in vitro and in vivo |
KR100845139B1 (ko) * | 2006-12-13 | 2008-07-09 | 전북대학교산학협력단 | 단백질-단백질 상호작용 측정을 위한 실시간 이중 색상분석방법 및 그 장치 |
EP2438555B1 (en) * | 2009-06-02 | 2017-03-08 | Sofast GmbH | Superresolution optical fluctuation imaging (sofi) |
US20100310246A1 (en) * | 2009-06-04 | 2010-12-09 | Digital Imaging Systems Gmbh | Method for using a variable aperture to tune image quality parameters in a camera system |
CN101587238B (zh) * | 2009-06-24 | 2012-01-04 | 中国科学院上海光学精密机械研究所 | 双色双光子荧光成像方法和装置 |
FR2951721A1 (fr) * | 2009-10-26 | 2011-04-29 | Univ Rouen | Procede de synthese d'analogues de l'epicocconone |
JP5484879B2 (ja) * | 2009-12-11 | 2014-05-07 | オリンパス株式会社 | 超解像顕微鏡 |
US8569680B2 (en) * | 2009-12-21 | 2013-10-29 | University Of Wyoming | Hyperacuity from pre-blurred sampling of a multi-aperture visual sensor |
US9946058B2 (en) * | 2010-06-11 | 2018-04-17 | Nikon Corporation | Microscope apparatus and observation method |
CN104364649B (zh) * | 2012-06-13 | 2017-05-17 | 默克专利股份有限公司 | 用于鉴定具有基因毒性的化合物的蛋白表达分析 |
JP6188721B2 (ja) * | 2012-12-13 | 2017-08-30 | オリンパス株式会社 | 蛍光観察装置 |
US10408757B2 (en) * | 2014-01-03 | 2019-09-10 | Arizona Board Of Regents On Behalf Of Arizona State University | Plasmonic imaging and detection of single DNA molecules |
WO2016090060A1 (en) * | 2014-12-02 | 2016-06-09 | Georgia Tech Research Corporation | Nucleophile-triggered degradble materials and methods of making and using the same |
-
2014
- 2014-12-17 CN CN201410787361.5A patent/CN104515760B/zh active Active
-
2015
- 2015-09-07 US US15/303,461 patent/US10151697B2/en active Active
- 2015-09-07 WO PCT/CN2015/089047 patent/WO2016095572A1/zh active Application Filing
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2535755A1 (en) * | 2011-06-14 | 2012-12-19 | Ecole Polytechnique Fédérale de Lausanne (EPFL) | Cumulant microscopy |
CN102566048A (zh) * | 2012-01-17 | 2012-07-11 | 浙江大学 | 一种基于象散的样品轴向漂移补偿方法和装置 |
CN102589428A (zh) * | 2012-01-17 | 2012-07-18 | 浙江大学 | 基于非对称入射的样品轴向位置跟踪校正的方法和装置 |
CN103399413A (zh) * | 2013-07-30 | 2013-11-20 | 浙江大学 | 基于双螺旋光束的样品轴向漂移检测及补偿方法和装置 |
CN103575716A (zh) * | 2013-11-15 | 2014-02-12 | 中国科学院长春应用化学研究所 | 磁场调控的超分辨荧光成像方法 |
CN103592278A (zh) * | 2013-11-21 | 2014-02-19 | 中国计量学院 | 基于荧光发射抑制机理的随机定位超分辨显微方法及装置 |
CN104089935A (zh) * | 2014-07-11 | 2014-10-08 | 中国科学院化学研究所 | 超分辨荧光寿命相关光谱*** |
CN104122662A (zh) * | 2014-08-15 | 2014-10-29 | 北京大学 | 一种超高密度超分辨光学闪烁显微成像***及方法 |
Non-Patent Citations (4)
Title |
---|
David Baddeley et al..4D super-resolution microscopy with conventional fluorophores and single wavelength excitation in optically thick cells and tissues.《PLoS ONE》.2011,第6卷(第5期),第e20645页. * |
Graham T Dempsey et al..Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging.《NATURE METHODS》.2011,第8卷(第12期),第1027-1036页. * |
Mark Bates et al..Multicolor super-resolution fluorescence imaging via multi-parameter fluorophore detection.《ChemPhyschem》.2012,第13卷第99-107页. * |
Y.Tang et al..Sub-nanometer drift correction for super-resolution imaging.《OPTICS LETTERS》.2014,第39卷(第19期),第5685-5688页. * |
Also Published As
Publication number | Publication date |
---|---|
US10151697B2 (en) | 2018-12-11 |
US20170284941A1 (en) | 2017-10-05 |
CN104515760A (zh) | 2015-04-15 |
WO2016095572A1 (zh) | 2016-06-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104515760B (zh) | 双色荧光定位超分辨率生物显微方法及*** | |
Vicidomini et al. | STED super-resolved microscopy | |
Turkowyd et al. | From single molecules to life: microscopy at the nanoscale | |
US8994807B2 (en) | Microscopy system and method for creating three dimensional images using probe molecules | |
US10394008B2 (en) | Hyperspectral multiphoton microscope for biomedical applications | |
CN108181282B (zh) | 一种三通道荧光定位超分辨率生物显微***及方法 | |
Ovečka et al. | Preparation of plants for developmental and cellular imaging by light-sheet microscopy | |
US20140340482A1 (en) | Three Dimensional Microscopy Imaging | |
WO2010108042A2 (en) | Non-coherent light microscopy | |
EP1584918A2 (de) | Verfahren und Vorrichtung zur Fluoreszenz-Lebensdauer-Imaging-Nanoskopie | |
Jing et al. | Super-resolution microscopy: shedding new light on in vivo imaging | |
Petriella et al. | Superresolution imaging with switchable fluorophores based on oxazine auxochromes | |
Talbot et al. | High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis | |
Watanabe et al. | Visualizing proteins in electron micrographs at nanometer resolution | |
Cavey et al. | Imaging cellular and molecular dynamics in live embryos using fluorescent proteins | |
Rai et al. | The basics of confocal microscopy | |
Waterman-Storer | Microtubules and microscopes: how the development of light microscopic imaging technologies has contributed to discoveries about microtubule dynamics in living cells | |
Kraus et al. | Linear fluorescence unmixing in cell biological research | |
DE102010035003B4 (de) | Räumlich und zeitlich hochauflösende Mikroskopie | |
KR20210122261A (ko) | 스펙트럼 언믹싱 | |
US9239292B2 (en) | Method and apparatus for tracking the motion of molecules in a microscope | |
White et al. | Multi-photon microscopy: seeing more by imaging less | |
Kanchanawong et al. | Localization-based super-resolution imaging of cellular structures | |
US20220228987A1 (en) | Clipping-Assisted Dual-Fluorophore Sensing | |
Quang et al. | Superresolution measurements in vivo: imaging Drosophila embryo by photoactivated localization microscopy |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Owner name: SHENZHEN NAGUAN BIOLOGY CO., LTD. Free format text: FORMER OWNER: HONG KONG NANOBIOIMAGING LTD. Effective date: 20150821 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20150821 Address after: 518000 Guangdong city of Shenzhen province Nanshan District Houhai Center Road, Liwan 100 E404 Applicant after: Shenzhen nano biological Co., Ltd. Address before: Room 6, building 2, 623, science and technology centre, 11 West Science Park, Sha Tin, Hongkong Science Park, Sha Tin, New Territories, Hongkong, China Applicant before: NANOBIOIMAGING LTD. |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20180428 Address after: 315300 Room 302, 6 Building 438, Hu Chong Road, Gutong street, Cixi, Zhejiang. Patentee after: Ningbo nanomei Biological Technology Co. Ltd. Address before: 518000 E404, Houhai Road, Nanshan District, Shenzhen, Guangdong. Patentee before: Shenzhen nano biological Co., Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20201105 Address after: 315400 No.5 Xingbin Road, Zhongyi Ningbo ecological park, Yuyao City, Ningbo City, Zhejiang Province (neighborhood center) 3-2-145 (self declaration) (limited to office) Patentee after: Ningbo Lixian Intelligent Technology Co., Ltd Address before: Room 302, building 6, 438 Huchong Road, Gutang street, Cixi City, Zhejiang Province, 315300 Patentee before: NINGBO NANOAMAZE BIOTECH Co.,Ltd. |