CN104431618A - Compound feed for preventing and treating gills bleeding of carassius auratus gibelio and application method of compound feed - Google Patents
Compound feed for preventing and treating gills bleeding of carassius auratus gibelio and application method of compound feed Download PDFInfo
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Abstract
The invention provides a compound feed for preventing and treating gills bleeding of carassius auratus gibelio. The compound feed is prepared from the following components in percentage by weight: 5%-10% of fish meal, 1%-15% of tenebrio molitor flour, 0.2%-10% of ginseng, 0.2%-5% of radix achyranthis bidentatae, 0.2%-5% of mushroom, 0%-10% of acanthopanax root, 0.2%-10% of herba epimedii, 0.2%-5% of liquorice, 20%-30% of cottonseed meal, 0%-5% of rapeseed meal, 5%-10% of bean pulp, 15%-20% of peanut meal, 5%-15% of soya-bean oil, 15%-20% of wheat, 0.2%-0.5% of choline chloride, 2% of monocalcium phosphate and 1.5% of gunk. Long-term breeding proves that the compound feed for preventing and treating gills bleeding of carassius auratus gibelio, prepared from an insect protein source and Chinese medicine raw materials as feed raw materials, is provided for the first time; and the effect of reducing gills bleeding is significant, thus the survival rate of breeding is significantly increased; and the compound feed is high in pertinence, good in effect, obvious in economic value and worthy of large-range popularization and application.
Description
Technical field
The invention belongs to aquatic feeds field, particularly relate to and a kind ofly prevent and treat the hemorrhage mixed feed of the hybridized prussian carp cheek and using method thereof.
Background technology
Carp herpes virus type 2 (Cyprinid herpesvirus 2, CyHV-2), also known as simplex keratitis Hematopoietic Necrosis virus, it is the cause of disease of the goldfish Hematopoietic Necrosis disease causing goldfish mortality, hybridized prussian carp (Carassius auratusgibelio) and goldfish belong to crucian carp (Carassius auratus) together and plant, identical to the neurological susceptibility of virus, be another natural host of CyHV-2.Hybridized prussian carp, because its delicious meat, growth are fast, output advantages of higher, are extensively cultivated in China, is occupied very important status in freshwater aquiculture.CyHV-2 infects the viral disease that the crucian Simplex Virus caused is the unique infection hybridized prussian carp found at present, the death rate is high, endanger huge, infectiousness is strong, during the annual 5-6 month and the 9-10 month, water temperature 20-28 DEG C, particularly water temperature is increased to 25 DEG C from 15 DEG C, can large area break out, be embodied in: the gill cover, fin ray, body surface, visceral hemorrhage, cheek silk epithelial cell shedding, haemocyte oozes out in a large number, cheek silk turns white, the most outstanding is the hematopoiesis organ pipe enlargements such as spleen kidney, the sick fish of stamping struggles a little and jumps, gill portion has blood to flow out (because the sick fish that lives is seldom so seldom see), the dead fish of dragging for up under water the side gill cover has watery blood to deposit, therefore be generally referred to as " cheek is hemorrhage ".2012 to 2014, at the Jianhu shining sun of jiangsu coast, the incidence of disease, up to 90%, does not also have the special effective prophylactico-therapeutic measures of prevention and controls for hybridized prussian carp " cheek is hemorrhage " so far, annual loss is huge, and this disease has become the chief threat of China's crucian cultivation.
Latent infection is one of principal character of herpesviral, can not show any symptom by long-term latent infection in fish body.It is this that to take viruliferous latent infection fish appearance normal, with virus-free healthy fish of carrying without any difference, be difficult to be identified differentiation, when temperature of cultivation is elevated to 22 DEG C, or other factors are when causing Abwehrkraft des Koepers to decline, latent infection is activated, and causes host start to fall ill and cause transmission, cause shoal of fish mortality, bring great difficulty therefore to the thorough elimination of disease.
The antiviral immunity of body is divided into two parts, i.e. specific immunity and nospecific immunity by classical Immunology.During evolution, fish are in the special status connecting higher vertebrate and the invertebrate such as low, its specific immune mechanisms imperfection, nospecific immunity accounts for critical role, after running into cause of disease, what first also play rapidly defense function is nonspecific immune response, for virus, significant nospecific immunity cell has two classes, one class is NK, and another kind of is mononuclear phagocyte system, meanwhile, some nonspecific humoral factor has also played important function, and that most represents meaning is interferon (INF).
At present, research in the feed formula of crucian, mainly for immunity and resisting stress aspect, such as number of patent application is 2013104440330,2013100802794, the application for a patent for invention of 201210117179X, also have in other, such as patent CN103330090B discloses a kind of Chinese herbal feed additive for infecting the explosive hemorrhage of hybridized prussian carp that Aeromonas hydrophila causes, and patent CN102008029B discloses a kind of Chinese medicine immunity enhancer preventing and treating hybridized prussian carp sporidiosis.So far, do not have a kind of specially for raising hybridized prussian carp anti-virus ability, thus improve the mixed feed of the hemorrhage function of the control hybridized prussian carp cheek.
Summary of the invention
For above deficiency, the invention provides a kind of ability improving hybridized prussian carp opposing virus infections, reduce mixed feed and the using method thereof of the hemorrhage generation of the cheek.
The present invention is achieved the above object by following scheme:
A kind ofly prevent and treat the hemorrhage mixed feed of the hybridized prussian carp cheek, by weight percentage, comprise following raw material components: 5 ~ 10% fish meal, 1 ~ 15% yellow meal worm powder, 0.2 ~ 10% ginseng, 0.2 ~ 5% root of bidentate achyranthes, 0.2 ~ 5% mushroom, 0 ~ 10% wilsonii, 0.2 ~ 10% barrenwort, 0.2 ~ 5% Radix Glycyrrhizae, 20 ~ 30% cotton dregs, 0 ~ 5% dish dregs of rice, 5 ~ 10% dregs of beans, 15 ~ 20% peanut meals, 5 ~ 15% soya-bean oil, 15 ~ 20% wheats, 0.2 ~ 0.5% Choline Chloride, 2% calcium dihydrogen phosphate, 1.5% premix.
The mixed feed that the preferred control hybridized prussian carp cheek is hemorrhage, by weight percentage, is made up of following raw material components: 9% fish meal, 5% yellow meal worm powder, 0.5% ginseng, 2% root of bidentate achyranthes, 2% mushroom, 0.5% barrenwort, 5% Radix Glycyrrhizae, 25% cotton dregs, the 5% dish dregs of rice, 6% dregs of beans, 15% peanut meal, 16% wheat, 0.5% Choline Chloride, 2% calcium dihydrogen phosphate, 1.5% premix, 5% soya-bean oil.
The mixed feed that the another kind of preferred control hybridized prussian carp cheek is hemorrhage, by weight percentage, is made up of following raw material components: 7% fish meal, 8% yellow meal worm powder, 1.0% ginseng, 1.0% root of bidentate achyranthes, 1.5% mushroom, 1.5% wilsonii, 2% barrenwort, 3% Radix Glycyrrhizae, 20% cotton dregs, the 4% dish dregs of rice, 7% dregs of beans, 18% peanut meal, 17.1% wheat, 0.4% Choline Chloride, 2% calcium dihydrogen phosphate, 1.5% premix, 5% soya-bean oil.
Above-mentioned premix can be commercially available general vitamin and minerals premix.
A kind of preferred premix is that every kilogram of premix comprises: vitamin A 2g, vitamin D 1g, vitamin E 6g, vitamin K 1g, nicotinic acid 6g, calcium pantothenate 4g, VB11 .5g, vitamin B2 1.8g, vitamin B6 1.8g, vitamin B12 0.012g, biotin 0.025g, folic acid 0.35g, vitamin C 6g, inositol 6g, five water magnesium sulfate 35g, ferrous sulfate monohydrate 20g, monohydrate zinc sulphate 20g, manganese sulfate monohydrate 6g, sodium selenite 4.5g, CoCL2 6H2O 1.6g, KI 5g, defatted rice bran 870.413g.
The present invention also provides a kind of method using the mixed feed that the above-mentioned control hybridized prussian carp cheek is hemorrhage, and described mixed feed and common hybridized prussian carp cheek feed interval are thrown something and fed, gap periods is 5-10 days.
Above-mentioned mixed feed, considering fish body nutritional need, meet on the basis of growth, utilize Chinese herbal medicine composition to improve hybridized prussian carp and produce antiviral agent (interleukins, interferon) ability, wherein, the panaxoside contained by ginseng and panaxan, glycyrrhizin contained by Radix Glycyrrhizae and enoxolone, epimedium brevicornum polysaccharide and flavones, Radix Et Caulis Acanthopanacis Senticosi polysaccharide and fly chew albumen and all can promote hybridized prussian carp interleukins, the generation of interferon, strengthens ability of killing the virus.
Raise through long-term raising and prove, the present invention proposes to utilize insect protein source and herbal raw material as feedstuff component for preventing and treating the hemorrhage mixed feed of the hybridized prussian carp cheek first, reduce the Be very effective of the hemorrhage incidence of disease of the cheek, thus significantly improve cultivation survival rate, with strong points, effective, economic worth is obvious, is worth applying on a large scale.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1:
Prevent and treat the mixed feed that the hybridized prussian carp cheek is hemorrhage, with weight, be made up of following raw material components: 9% fish meal, 5% yellow meal worm powder (i.e. the dry worm powder of yellow meal worm), 0.5% ginseng, 2% root of bidentate achyranthes, 2% mushroom, 0.5% barrenwort, 5% Radix Glycyrrhizae, 25% cotton dregs, the 5% dish dregs of rice, 6% dregs of beans, 15% peanut meal, 16% wheat, 0.5% Choline Chloride, 2% calcium dihydrogen phosphate, 1.5% premix, 5% soya-bean oil.
Wherein, above-mentioned premix is every kilogram of premix: vitamin A 2g, vitamin D 1g, vitamin E 6g, vitamin K 1g, nicotinic acid 6g, calcium pantothenate 4g, VB11 .5g, vitamin B2 1.8g, vitamin B6 1.8g, vitamin B12 0.012g, biotin 0.025g, folic acid 0.35g, vitamin C 6g, inositol 6g, five water magnesium sulfate 35g, ferrous sulfate monohydrate 20g, monohydrate zinc sulphate 20g, manganese sulfate monohydrate 6g, sodium selenite 4.5g, CoCL2 6H2O 1.6g, KI 5g, defatted rice bran 870.413g.
The preparation of above-mentioned mixed feed: mixing after the raw material components of above-mentioned mixed feed is pulverized, and namely obtain mixed feed through modified, processing of granulating.
Embodiment 2:
Prevent and treat the mixed feed that the hybridized prussian carp cheek is hemorrhage, with weight, be made up of following raw material components: 7% fish meal, 8% yellow meal worm powder (i.e. the dry worm powder of yellow meal worm), 1.0% ginseng, 1.0% root of bidentate achyranthes, 1.5% mushroom, 1.5% wilsonii, 2% barrenwort, 3% Radix Glycyrrhizae, 20% cotton dregs, the 4% dish dregs of rice, 7% dregs of beans, 18% peanut meal, 17.1% wheat, 0.4% Choline Chloride, 2% calcium dihydrogen phosphate, 1.5% premix, 5% soya-bean oil.
Wherein, above-mentioned premix is every kilogram of premix: vitamin A 2g, vitamin D 1g, vitamin E 6g, vitamin K 1g, nicotinic acid 6g, calcium pantothenate 4g, VB11 .5g, vitamin B2 1.8g, vitamin B6 1.8g, vitamin B12 0.012g, biotin 0.025g, folic acid 0.35g, vitamin C 6g, inositol 6g, five water magnesium sulfate 35g, ferrous sulfate monohydrate 20g, monohydrate zinc sulphate 20g, manganese sulfate monohydrate 6g, sodium selenite 4.5g, CoCL2 6H2O 1.6g, KI 5g, defatted rice bran 870.413g.
Embodiment 3:
The effect test of embodiment 1:
In Yancheng, Jiangsu Province shining sun hybridized prussian carp cultivation market, the mixed feed of embodiment 1 adds up to 3000 tons, be applied in about 4000 mu of waters surface, add up to 23 raisers, in process of the test, every mu of water surface is is on average thrown something and fed mixed feed about 1.5 tons, and wherein the mixed feed of embodiment 1 accounts for 1 ton, and other 0.5 ton of feed is commercially available hybridized prussian carp feed.Specifically the mode of throwing something and feeding is: the mixed feed of 10 days embodiments 1 of throwing something and feeding at interval of 5 days, and 5 day time at interval throws something and feeds commercially available hybridized prussian carp feed.
Result shows, adopt the test group generation whole year " cheek is hemorrhage " 6 family of the mixed feed of embodiment 1, incidence 27.3%, cultivation survival rate 66.5%, and whole shining sun market (adopting commercially available hybridized prussian carp feed normally to feed and the mixed feed of embodiment 1 of not throwing something and feeding) " cheek is hemorrhage " incidence of disease 81.3% in addition, average survival is 32.2%.Wherein, commercially available hybridized prussian carp feed is as shown in table 1.
Table 1: commercially available hybridized prussian carp feed formula table
| Component | Weight percentage (%) |
| Fish meal | 20 |
| Dregs of beans | 15 |
| The dish dregs of rice | 15 |
| Cotton dregs | 5 |
| DDGS | 7 |
| Rice bran | 8 |
| Wheat | 20 |
| System chaff | 2.3 |
| Calcium dihydrogen phosphate | 2 |
| Choline Chloride | 0.2 |
| Soya-bean oil | 3 |
| Phosphatide oil | 1 |
| Premix | 1.5 |
Embodiment 4:
The effect test of embodiment 2:
In Yancheng, Jiangsu Province great Feng hybridized prussian carp cultivation market, the mixed feed of embodiment 2 adds up to 4000 tons, be applied in about 4000 mu of waters surface, add up to 16 raisers, in process of the test, every mu of water surface is is on average thrown something and fed mixed feed about 2 tons, the wherein mixed feed of embodiment 2 about 1 ton, and other 1 ton of feed is commercially available hybridized prussian carp feed.Specifically the mode of throwing something and feeding is the mixed feed of 10 days embodiments 2 of throwing something and feeding at interval of 10 days, and 10 day time at interval throws something and feeds commercially available hybridized prussian carp feed.
Result shows, adopt the test group generation whole year " cheek is hemorrhage " 3 family of the mixed feed of embodiment 2, incidence 18.8%, cultivation survival rate 76.5%, and field, whole Dafeng City (adopting commercially available hybridized prussian carp feed normally to feed and the mixed feed of embodiment 2 of not throwing something and feeding) " cheek is hemorrhage " incidence of disease 75.3% in addition, average survival is 35.2%.Wherein, commercially available hybridized prussian carp feed is as shown in table 1.
Embodiment 5:
The effect test of embodiment 2:
In Yancheng, Jiangsu Province shining sun hybridized prussian carp cultivation market, the mixed feed (test group) of embodiment 2 and each 240 tons of hybridized prussian carp conventional feed (control group), be shown in Table 2, be applied in the water surface of about 160 mu respectively, wherein test group pond Volume Composition 15 mouthfuls, conventional feed (control group) pond Volume Composition 12 mouthfuls.Every mu of water surface is is on average thrown something and fed mixed feed about 1.5 tons.Specifically the mode of throwing something and feeding is: the mixed feed filled a prescription at interval of the 10 days embodiments 2 of throwing something and feeding for 5 days in the pond of test group, the hybridized prussian carp conventional feed of 5 days control groups of throwing something and feeding at interval, and throws something and feeds the hybridized prussian carp conventional feed of control group in the pond of contrast groups always.Result shows, " cheek the is hemorrhage " incidence 16.3% in the pond of experimental group, and cultivation survival rate 81.6%, pond " cheek the is hemorrhage " incidence of disease 59.2% of the contrast groups of conventional feed of throwing something and feeding, average survival is 42.5%.
Table 2: the feed formula table of experimental group and contrast groups
| Experimental group | Control group | |
| Fish meal | 7 | 22 |
| Peanut meal | 18 | 3 |
| Yellow meal worm | 8 | -- |
| Dregs of beans | 7 | 15 |
| The dish dregs of rice | 4 | 15 |
| Cotton dregs | 20 | 5 |
| DDGS | 0 | 6 |
| Rice bran | 0 | 7 |
| Wheat | 17.1 | 21.1 |
| Ginseng | 1 | 0 |
| The root of bidentate achyranthes | 1 | 0 |
| Mushroom | 1.5 | 0 |
| Wilsonii | 1.5 | 0 |
| Barrenwort | 2 | 0 |
| Radix Glycyrrhizae | 3 | 0 |
| Calcium dihydrogen phosphate | 2 | 2 |
| Choline Chloride | 0.4 | 0.4 |
| Soya-bean oil | 5 | 2 |
| Premix | 1.5 | 1.5 |
The above; be only preferably specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and design thereof and replace or change, all should be encompassed in protection scope of the present invention.
Claims (4)
1. prevent and treat the hemorrhage mixed feed of the hybridized prussian carp cheek for one kind, it is characterized in that, by weight percentage, comprise following raw material components: 5 ~ 10% fish meal, 1 ~ 15% yellow meal worm powder, 0.2 ~ 10% ginseng, 0.2 ~ 5% root of bidentate achyranthes, 0.2 ~ 5% mushroom, 0 ~ 10% wilsonii, 0.2 ~ 10% barrenwort, 0.2 ~ 5% Radix Glycyrrhizae, 20 ~ 30% cotton dregs, 0 ~ 5% dish dregs of rice, 5 ~ 10% dregs of beans, 15 ~ 20% peanut meals, 5 ~ 15% soya-bean oil, 15 ~ 20% wheats, 0.2 ~ 0.5% Choline Chloride, 2% calcium dihydrogen phosphate, 1.5% premix.
2. a kind ofly prevent and treat the hemorrhage mixed feed of the hybridized prussian carp cheek as claimed in claim 1, it is characterized in that, by weight percentage, be made up of following raw material components: 9% fish meal, 5% yellow meal worm powder, 0.5% ginseng, 2% root of bidentate achyranthes, 2% mushroom, 0.5% barrenwort, 5% Radix Glycyrrhizae, 25% cotton dregs, the 5% dish dregs of rice, 6% dregs of beans, 15% peanut meal, 16% wheat, 0.5% Choline Chloride, 2% calcium dihydrogen phosphate, 1.5% premix, 5% soya-bean oil.
3. a kind ofly prevent and treat the hemorrhage mixed feed of the hybridized prussian carp cheek as claimed in claim 1, it is characterized in that, by weight percentage, be made up of following raw material components: 7% fish meal, 8% yellow meal worm powder, 1.0% ginseng, 1.0% root of bidentate achyranthes, 1.5% mushroom, 1.5% wilsonii, 2% barrenwort, 3% Radix Glycyrrhizae, 20% cotton dregs, the 4% dish dregs of rice, 7% dregs of beans, 18% peanut meal, 17.1% wheat, 0.4% Choline Chloride, 2% calcium dihydrogen phosphate, 1.5% premix, 5% soya-bean oil.
4. a using method for the mixed feed that the control hybridized prussian carp cheek as described in claim arbitrary in claim 1-3 is hemorrhage, is characterized in that, throw something and feed in described mixed feed and common hybridized prussian carp cheek feed interval, gap periods is 5-10 days.
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Cited By (7)
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| CN107410753A (en) * | 2017-03-28 | 2017-12-01 | 陆川县恒伟养殖专业合作社 | A kind of selenium-rich fish meal and preparation method thereof |
| CN107897576A (en) * | 2017-11-21 | 2018-04-13 | 无锡三智生物科技有限公司 | A kind of hybridized prussian carp feed for preventing carp herpesvirusⅡtype virus |
| CN108925781A (en) * | 2017-05-26 | 2018-12-04 | 无锡三智生物科技有限公司 | Hybridized prussian carp feed addictive and its preparation method and application |
| CN109463580A (en) * | 2018-12-31 | 2019-03-15 | 盐城工学院 | A kind of hybridized prussian carp feed and preparation method thereof |
| CN111296632A (en) * | 2020-02-24 | 2020-06-19 | 盐城工学院 | Chinese herbal medicine compound preparation for preventing and treating gibelism hemorrhage of carassius auratus gibelio and preparation method thereof |
| CN112167123A (en) * | 2020-09-27 | 2021-01-05 | 通威股份有限公司 | Carassius auratus gibelio breeding method for reducing incidence rate of gill bleeding of carassius auratus |
| CN113559118A (en) * | 2021-08-04 | 2021-10-29 | 中国科学院水生生物研究所 | Application of ferrous ions in preventing and treating carp herpesvirus II (CyHV2) infection |
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