CN104386813B

CN104386813B - A kind of method of quick formation anaerobic ammonium oxidation granular sludge

Info

Publication number: CN104386813B
Application number: CN201410668873.XA
Authority: CN
Inventors: 于鹏飞; 苏杨; 赵鹤谦; 高子平
Original assignee: Shenyang Jianzhu University
Current assignee: Shenyang Jianzhu University
Priority date: 2014-11-20
Filing date: 2014-11-20
Publication date: 2016-01-13
Anticipated expiration: 2034-11-20
Also published as: CN104386813A

Abstract

A method for quick formation anaerobic ammonium oxidation granular sludge, belongs to biological wastewater treatment field.Reactor with sewage work's digested sludge for seed sludge is inoculated UASB reactor; Wherein reactor MLSS is 3672mg/L, MLVSS be 2398mg/L, MLVSS/MLSS is 65.3%; Reactor exospheric temperature controls at 32+1 DEG C, with inorganic carbon source control pH 7.8 ~ 8.2; Keep the condition such as temperature, pH of reactor.By exposing to the sun nitrogen and add metallic cation compound continuously, after steady running for some time, obtain red anaerobic ammonium oxidation granular sludge.It is simple that present method has operational condition, the advantages such as the anaerobic ammonium oxidation granulation time is short, can be widely used in forming anaerobic ammonium oxidation sludge granulating.

Description

A kind of method of quick formation anaerobic ammonium oxidation granular sludge

Technical field

The present invention relates to ANAMMOX granular sludge technology, belong to biological wastewater treatment field.Be intended to the method for openly a kind of quick formation ANAMMOX granule sludge.

Background technology

Anammox be in recent years in the efficient bio-denitrification technology that grows up, compared with traditional nitration denitrification technique, it does not need additional organic carbon source to carry out denitrification, and sludge yield is few; Do not need oxygen completely, save a large amount of working costs; Do not need acid-base neutralisation agent, avoid secondary pollution.Anammox is particularly suitable for process low-carbon (LC) high ammonia-nitrogen wastewater, therefore inquires into Anammox process and is significant to efficient process ammonia nitrogen waste water.

Anaerobic ammonia oxidizing bacteria poor growth, cell yield is low, and the feature extremely responsive to environment makes it be difficult to carry out large-scale application in engineering practice.How to obtain the technical essential that enough anaerobic ammonia oxidizing bacterias are Anammox most criticals.Granular sludge can improve the settling property of thalline and improve the structure of reactor, and impelling function yeast effectively to hold and stay, is prerequisite and the guarantee of realization response device Effec-tive Function.Therefore how fast culture efficient anaerobic ammoxidation granule sludge has important practical significance.

Summary of the invention

Longer and water inlet flow shear is not enough for the existing formation anaerobic ammonium oxidation granular sludge cycle, the problem to be solved in the present invention is to provide a kind of method and the operation of granular sludge reactor when forming certain hydraulic shear of quick formation anaerobic ammonium oxidation granular sludge.

The method of quick formation anaerobic ammonium oxidation granular sludge of the present invention, its step related to and processing condition are:

1) reactor is with sewage work's digested sludge for seed sludge is inoculated UASB reactor, and mud is black, device steady running 2 years, mud gray, and is mingled with red floc sludge.Wherein reactor MLSS is 3672mg/L, MLVSS be 2398mg/L, MLVSS/MLSS is 65.3%, mud free from extraneous odour.2) reactor exospheric temperature controls 32 +1 DEG C.With inorganic carbon source control pH 7.8 ~ 8.2.

3) described UASB reactor material is synthetic glass, is divided into sludge reaction district, sludge settling district, mud-water separation district three part.There is inside and outside double-layer structure in sludge reaction district: internal layer is reaction zone, internal diameter 10cm, height overall 200cm, reaction zone volume 11L, settling region volume 4L.Outer is water bath with thermostatic control district, is ensure that anaerobic ammonia oxidizing bacteria has suitable growing environment, takes lucifuge process to reactor with temperature controller controlled circulation bath temperature.For making anaerobic ammonia oxidation reactor have more excellent treatment effect, the secondary escape pipe of traditional UASB reactor addition, and built-in two triphase separators.

4) with the water inlet that the simulated wastewater manually prepared is reactor, waterpower lift velocity is 1 ~ 2m/h, and reactor hydraulic detention time is 12h; Wherein, NH ₄-N, NO ₂-N is respectively with NH ₄cl, NaNO ₂there is provided, KH ₂pO ₄concentration be 10mg/L, MgSO ₄7H ₂the concentration of O is 300mg/L, CaCl ₂2H ₂the concentration of O is 56mg/L; The each 1mL of trace element I, II joins in 1L water inlet, and the concentration of micro-I:EDTA is 5000mg/L, FeSO ₄concentration be 5000mg/L; The concentration of trace element II:EDTA is 15000mg/L, ZnSO ₄7H ₂the concentration of O is 430mg/L, MnCl ₂4H ₂the concentration of O is 990mg/L, CH ₃bO ₄concentration be 14mg/L, CuSO ₄5H ₂the concentration of O is 250mg/L, NaMoO ₄2H ₂o concentration is 220mg/L, NaSeO ₄10H ₂o concentration is 210mg/L, NiCl ₂6H ₂the concentration of O is 190mg/L;

5) reaction adopts Continuous Flow mode to run, and before test starts, in reactor water inlet, the concentration ratio of ammonia nitrogen and nitrite nitrogen is 1:1.1 ~ 1.2; In water inlet, the concentration of ammonia nitrogen is 150mg/L, and the concentration of nitrite nitrogen is 177mg/L.Within every two days, survey nitrogen concentration, PH, according to its adjustment reactor operational conditions.

6) in reactor, pass into nitrogen continuously, gas-water ratio is 10:1.Under stronger hydraulics, can form closely knit granule sludge, the effect of mass transmitting of enhancement microbiological and pollutent, not easily disintegrates.Hydraulic shear is the coefficient result of gas, liquid, solid three-phase.

7) in reactor, metallic cation compound is added: metallic cation compound is by FeSO ₄, CaCl ₂2H ₂o and MgSO ₄7H ₂o forms; FeSO ₄concentration be 50 ~ 110mg/L, CaCl ₂2H ₂the concentration of O is 40 ~ 70mg/L, MgSO ₄7H ₂the concentration of O is 60 ~ 150mg/L.

Beneficial effect: because cell is electronegative, positively charged ion can form a positive charge layer at cell surface, and to affect between thalline with matrix effective contacts for the dense degree of positive charge layer, thus can affect the metabolic process of thalline.Iron ion can promote the nitric efficiency of reactor, and reactor maximum removal rate increases with the rising of iron concentration; Calcium constituent is that Anammox bacteria growing is very important.Adding of calcium constituent can increase the settleability of mud.Anaerobic ammonium oxidation sludge must have good settleability.Due to the nutrition goods and materials that namely ammonia-state nitrogen in Anammox reaction and nitrite nitrogen itself are reactions, it is again inhibitory substance.So want the clearance improving nitrogen, have only and realized by reduction hydraulic detention time or enlargement discharge, this just increases the requirement of anaerobic ammonium oxidation granular sludge to sludge settling.Magnesium elements is the important component in anaerobic grain sludge ash content, can the form of carbonate be present in granule sludge, can improve the settleability to granule sludge.

Accompanying drawing explanation

Fig. 1 is reaction unit figure;

Fig. 2 is that hydraulic shear is to anaerobic ammonia oxidizing bacteria effect;

Fig. 3 is that metallic cation is to anaerobic ammonia oxidizing bacteria effect.

Embodiment

Of the present inventionly carry out step and processing condition are:

1) in reactor, pass into nitrogen remove oxygen in water inlet, reactor exospheric temperature controls at 32+1 DEG C, with inorganic carbon source control pH 7.8 ~ 8.2;

2) UASB reactor material is synthetic glass, is divided into sludge reaction district, sludge settling district, mud-water separation district three part.There is inside and outside double-layer structure in sludge reaction district: internal layer is reaction zone, internal diameter 10cm, height overall 200cm, reaction zone volume 11L, settling region volume 4L.Outer is water bath with thermostatic control district, is ensure that anaerobic ammonia oxidizing bacteria has suitable growing environment, takes lucifuge process to reactor with temperature controller controlled circulation bath temperature.

3) with the water inlet of the simulated wastewater manually prepared for UASB reactor, wherein, NH ₄-N and NO ₂-N is respectively with NH ₄cl, NaNO ₂there is provided, NaHCO ₃have for anaerobic ammonia oxidizing bacteria provides inorganic carbon source and the effect regulating pH.ρ (KH ₂pO ₄)=10mg/L, ρ (MgSO ₄7H ₂o)=300mg/L, ρ (CaCl ₂2H ₂o)=56mg/L; The each 1mL of trace element I, II adds in 1L water inlet, micro-I: ρ (EDTA)=5000mg/L, ρ (FeSO ₄)=5000mg/L; Trace element II: ρ (EDTA)=15000mg/L, ρ (ZnSO ₄7H ₂o)=430mg/L, ρ (MnCl ₂4H ₂o)=990mg/L, CH ₃bO ₄)=14mg/L, ρ (CuSO ₄5H ₂o)=250mg/L, ρ [(NaMoO ₄2H ₂o]=220mg/L, ρ [(NaSeO ₄10H ₂o]=210mg/L, ρ (NiCl ₂6H ₂o)=190mg/L.

4) reaction adopts Continuous Flow mode to run, and before test starts, the concentration of reactor influent ammonia nitrogen and nitrite nitrogen is respectively 150mg/L, 177mg/L.Within every two days, survey nitrogen concentration, PH, according to its adjustment reactor operational conditions.

5) in reactor, pass into nitrogen continuously, gas-water ratio is 10:1.Under stronger hydraulics, densification, solid granule sludge can be formed, the effect of mass transmitting of enhancement microbiological and pollutent.Hydraulic shear is the coefficient result of gas, liquid, solid three-phase.

6) in reactor, metallic cation compound is added: ρ (FeSO ₄)=50 ~ 110mg/L, ρ (CaCl ₂2H ₂o)=40 ~ 70mg/L, ρ (MgSO ₄7H ₂o)=60 ~ 150mg/L.

Wherein:

Reactor is with sewage work's digested sludge for seed sludge is inoculated UASB reactor, and mud is black, device steady running 2 years, mud gray, and is mingled with red floc sludge.

In quick formation anaerobic ammonium oxidation granular sludge method, reactor MLSS is 3672mg/L, MLVSS be 2398mg/L, MLVSS/MLSS is 65.3%, mud free from extraneous odour.

In quick formation anaerobic ammonium oxidation granular sludge method, in water inlet, the concentration ratio of ammonia nitrogen and nitrite nitrogen is 1:1.1 ~ 1.2.

In quick formation anaerobic ammonium oxidation granular sludge method, in order to make reactor have more excellent treatment effect, to the secondary escape pipe of traditional UASB reactor addition, and built-in two triphase separators.

By exposing to the sun nitrogen and add metallic cation compound continuously, after steady running for some time, obtain red anaerobic ammonium oxidation granular sludge.It is simple that present method has operational condition, the advantages such as the anaerobic ammonium oxidation granulation time is short, can be widely used in forming anaerobic ammonium oxidation sludge granulating.

Claims

1. form a method for anaerobic ammonium oxidation granular sludge fast, it is characterized in that comprising the following steps:

1) reactor with sewage work's digested sludge for seed sludge is inoculated UASB reactor; Wherein reactor MLSS is 3672mg/L, MLVSS be 2398mg/L, MLVSS/MLSS is 65.3%;

2) reactor exospheric temperature controls at 32 ± 1 DEG C, with inorganic carbon source control pH 7.8 ~ 8.2;

3) with the water inlet that the simulated wastewater manually prepared is reactor, waterpower lift velocity is 1 ~ 2m/h, and reactor hydraulic detention time is 12h; Wherein, NH ₄-N, NO ₂-N is respectively with NH ₄cl, NaNO ₂there is provided, KH ₂pO ₄concentration be 10mg/L, MgSO ₄7H ₂the concentration of O is 300mg/L, CaCl ₂2H ₂the concentration of O is 56mg/L;

4) reaction adopts Continuous Flow mode to run, NH in reactor water inlet before test starts ₄-N and NO ₂the concentration ratio of-N is 1:1.1 ~ 1.2;

5) in reactor, pass into nitrogen continuously, maintenance gas-water ratio is 10:1; Metallic cation compound is added in reactor.

2. the method for a kind of quick formation anaerobic ammonium oxidation granular sludge as claimed in claim 1, is characterized in that: in reactor, inorganic carbon source is NaHCO ₃, for anaerobic ammonia oxidizing bacteria provides inorganic carbon source and adjust ph.

3. the method for a kind of quick formation anaerobic ammonium oxidation granular sludge as claimed in claim 1, is characterized in that: add each 1mL of micro-I, II in every 1L water inlet, the concentration of micro-I:EDTA is 5000mg/L, FeSO ₄concentration be 5000mg/L; The concentration of trace element II:EDTA is 15000mg/L, ZnSO ₄7H ₂the concentration of O is 430mg/L, MnCl ₂4H ₂the concentration of O is 990mg/L, CH ₃bO ₄concentration be 14mg/L, CuSO ₄5H ₂the concentration of O is 250mg/L, NaMoO ₄2H ₂o concentration is 220mg/L, NaSeO ₄10H ₂o concentration is 210mg/L, NiCl ₂6H ₂the concentration of O is 190mg/L.

4. the method for a kind of quick formation anaerobic ammonium oxidation granular sludge as claimed in claim 1, is characterized in that: reactor run duration continues to reactor assembly the nitrogen that exposes to the sun.

5. the method for a kind of quick formation anaerobic ammonium oxidation granular sludge as claimed in claim 1, is characterized in that: metallic cation compound is by FeSO ₄, CaCl ₂2H ₂o and MgSO ₄7H ₂o forms; FeSO ₄concentration be 50 ~ 110mg/L, CaCl ₂2H ₂the concentration of O is 40 ~ 70mg/L, MgSO ₄7H ₂the concentration of O is 60 ~ 150mg/L.

6. the method for a kind of quick formation anaerobic ammonium oxidation granular sludge as claimed in claim 1, is characterized in that: NH in water inlet ₄the concentration of-N is 150mg/L, NO ₂the concentration of-N is 177mg/L.