CN104365220A - Cyclocarya paliurus seed treatment method - Google Patents

Cyclocarya paliurus seed treatment method Download PDF

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Publication number
CN104365220A
CN104365220A CN201410771271.7A CN201410771271A CN104365220A CN 104365220 A CN104365220 A CN 104365220A CN 201410771271 A CN201410771271 A CN 201410771271A CN 104365220 A CN104365220 A CN 104365220A
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seed
blue
money willow
green money
days
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CN201410771271.7A
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CN104365220B (en
Inventor
孙远东
王海华
向育君
胡伟
谭树华
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Jiangsu Yangxian Technology Co ltd
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Hunan University of Science and Technology
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Abstract

The invention discloses a cyclocarya paliurus seed treatment method and belongs to the field of tree reproduction. The method includes the steps: (1) collecting seeds, removing fruiting wings and grinding thin seed coats with a mechanical method; (2) rubbing and elutriating the seeds in clear water added with alkaline synthetic detergent for six to eight times for removing part of inhibitors in the seed coats; (3) adopting the low-warm-low temperature-variable stratification and plant growth regulator (gibberellin) treatment combined dormancy release technique for accelerating germination. Inhibition of cyclocarya paliurus seed germination is eliminated or alleviated by the above taken measures, germination of the cyclocarya paliurus seeds is accelerated and increased, and high germination rate of 78.1-82.6% is obtained. By the technique, the seeds harvested in the current year can be sowed for seedling raising in the next spring.

Description

A kind of processing method of blue or green money willow seed
Technical field
The invention belongs to tree propagation field, specifically, be to provide a kind of processing method of blue or green money willow seed.
Background technology
Blue or green money willow ( cyclocarya paliurus(Batal) Iljinskaja) be the distinctive Rare plant of China; containing multiple compounds such as polysaccharide, triterpene compound, steroid compound, flavones in blue or green money willow; have and reduce blood sugar and blood fat, antitumor and anti-ageing several functions of waiting for a long time; being good natural health care and medicine resource, is a kind of rare tree having very much DEVELOPMENT PROSPECT.Blue or green money willow seed has deep dormancy characteristic, needs next year even just to sprout after 2 years after conventional seed planting, and natural germination rate extremely low (less than 0.2%), investigation finds, in the area of blue or green money willow, because percentage of seedgermination is low, its natural regeneration ability is more weak, is difficult to find treelet seedling.Existing blue or green money willow natural resources is few, and seedling breeding technology not yet obtains the breakthrough of essence, seriously constrains the development and utilization of blue or green money willow, and the artificial propagation techniques therefore studying blue or green money willow becomes the task of top priority developing blue or green money willow.Research shows that the rigid shell of blue or green money willow seed causes its main cause of forcing dormancy, secondly, seed also also exists physiological dormancy, seed coat hinders embryo and extraneous water_air exchange to a certain extent, in addition, have accumulated a large amount of inhibiting substances in seed coat, also make rataria penetration depth resting state.Therefore, as long as take corresponding measure eliminate or alleviate the above-mentioned influence factor causing blue or green money willow percentage of seedgermination too low, blue or green money willow percentage of seedgermination just effectively can be improved, thus the nursery speed of Quick Gaoqing Qian Liu.
Summary of the invention
The object of the invention is to solve blue or green money willow seed next year could sprout under field conditions (factors), and the problem that germination rate is low, a kind of processing method of blue or green money willow seed is provided, blue or green money willow percentage of seedgermination can not only be improved, can also realize collecting seed then, next year can the target of seeding and seedling raising, solves blue or green money willow seed seedling-raising technical barrier.
In order to achieve the above object, the present invention adopts following technical scheme: a kind of processing method of blue or green money willow seed, comprises the following steps:
(1) collect seed October, remove fruit wing, mechanically wear down seed coat;
(2) blue or green money willow seed step (1) processed crumples elutriation 6 ~ 8 times in the clear water of washing agent, with the liquor potassic permanganate of 50ppm seed soaking sterilization 0.5 hour, cleans and dries;
(3) blue or green money willow seed clear water step (2) processed soaks 4 ~ 6 days, is filtered dry, soaks 1 day by 400ppm Gibberellins solution, then the wet sand mixed is soaked as lamination matrix with 400ppm gibberellin, husky was 3:1 with the volume ratio of seed, loads antistaling box, in 2 ~ 6 DEG C of refrigerator laminations 30 ~ 35 days; Then sift out seed, then with the wet sand mixing that the leaching of 400ppm gibberellin is mixed, be placed in 20 ~ 30 DEG C of constant incubator laminations 40 ~ 50 days; And then with the wet sand mixing that the leaching of 400ppm gibberellin is mixed, to be placed in-2 ~-20 DEG C of low temperature refrigerators lamination 30 ~ 35 days.
As preferably, step mechanically grinds off 1/3 ~ 2/3 exosper in (1).
As preferably, the washing agent in step (2) is alkaline synthetic detergent.
In step (3), in Stratificated treatment process, often stir inspection, keep seed moistening, note mildew-resistant.
Further optimization, in step (3), prior to 4 DEG C of refrigerator laminations, is then placed in 20 DEG C of constant incubator laminations, and then is placed at lamination in-4 ~-20 DEG C of low temperature refrigerators.
Beneficial effect: mechanically wear down seed coat, can destroy blue or green money willow seed skin structure to a certain extent, alleviate it to the mechanical obstacles of seed germination and permeability obstacle.Blue or green money willow seed is crumpled elutriation 6 ~ 8 times adding in alkaline synthetic detergent clear water, part mortifier in seed coat can be removed.Treated seed effectively can depart from dormancy and suppress, as long as soil environment is suitable for, within after planting 30 ~ 40 days, can sprout, germination rate reaches 78.1 ~ 82.6%, and it is neat to emerge, and survival rate is high.This technology can realize the seed of gathering then, and spring next year gets final product seeding and seedling raising.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1:
1, collect seed: be gather ripe blue or green money willow seed in 2012.
2, seed treatment:
(1) after seed is adopted back, remove wing, on coarse concrete floor, exchange views seed with coarse sandpaper and wooden float, grind off most of exocuticle.
(2) the seed clear water through mill skin process soaks, and adds washing powder rubbing pericarp 6 times, with the liquor potassic permanganate seed soaking sterilization 0.5 hour of 50ppm, cleans and dries in the shade.
(3) low-warm-low alternating temperature stratification is adopted to process breaking dormancy technology in conjunction with plant growth regulator (gibberellin), carry out vernalization process.Concrete grammar is: the blue or green money willow seed processed in step (2) is soaked 4 days at clear water, be filtered dry, soak 24 hours by 400ppm Gibberellins solution, then with the wet sand mixing that the leaching of 400ppm gibberellin is mixed, load plastic storage case, be placed in 4 DEG C of refrigerator Stratificated treatment 30 days.Then chest is placed in 20 DEG C of constant incubator Stratificated treatment 40 days, moisturizing is ventilated, and keeps humidity about 70%.To be placed in-4 DEG C of low temperature refrigerators lamination 30 days.
3, feeding block seedlings raising
In late March, 2013, adopts the nursery of black nutritive cup.Matrix is the mixture of red soil and vermiculite, and its weight ratio is 7:1.Red soil takes from the soil layer of upper soll layer 5 ~ below 10cm, dries, and breaks into pieces, sieves, and after fully mixing in proportion, sprays 2 times, carry out matrix sterilization, sow front water and irrigate, be placed in plastic tunnel before dress cup with carbendazim with vermiculite.Within every two days later, use water spray moisturizing.
This method after planting 30 days, germination rate is 78.1%.
Embodiment 2:
1, collect seed: be gather ripe blue or green money willow seed in 2013.
2, seed treatment:
(1) after seed is adopted back, remove wing, on coarse concrete floor, exchange views seed with coarse sandpaper and wooden float, grind off most of exocuticle.
(2) the seed clear water through mill skin process soaks, and adds washing powder rubbing pericarp 8 times, with the liquor potassic permanganate seed soaking sterilization 0.5 hour of 50ppm, cleans and dries in the shade.
(3) low-warm-low alternating temperature stratification is adopted to process breaking dormancy technology in conjunction with plant growth regulator (gibberellin), carry out vernalization process.Concrete grammar is: the blue or green money willow seed processed in step (2) is soaked 5 days at clear water, be filtered dry, soak 24 hours by 400ppm Gibberellins solution, then with the wet sand mixing that the leaching of 400ppm gibberellin is mixed, load plastic storage case, be placed in 2 DEG C of refrigerator Stratificated treatment 32 days.Then chest is placed in 25 DEG C of constant incubator Stratificated treatment 45 days, moisturizing is ventilated, and keeps humidity about 70%.To be placed in-10 DEG C of low temperature refrigerators lamination 30 days.
3, drilling is germinateed
In late March, 2014, carries out drilling by gained seed after process, and seed furrow is apart from 25cm, dark 3cm, thickness of earth covering 2cm, and matrix is the mixture of red soil and vermiculite, and its weight ratio is 7:1.Red soil takes from the soil layer of upper soll layer 5 ~ below 10cm, breaks into pieces, after fully mixing in proportion, sprays 2 times with carbendazim, carry out soil disinfection with vermiculite, sows front water and irrigates, covered plastic booth.To spray water later every day moisturizing.
After planting about 30d, germination rate is 82.6%.
Embodiment 3:
1, collect seed: be gather ripe blue or green money willow seed in 2013.
2, seed treatment:
(1) after seed is adopted back, remove wing, on coarse concrete floor, exchange views seed with coarse sandpaper and wooden float, grind off most of exocuticle.
(2) the seed clear water through mill skin process soaks, and adds washing powder rubbing pericarp 8 times, with the liquor potassic permanganate seed soaking sterilization 0.5 hour of 50ppm, cleans and dries in the shade.
(3) low-warm-low alternating temperature stratification is adopted to process breaking dormancy technology in conjunction with plant growth regulator (gibberellin), carry out vernalization process.Concrete grammar is: the blue or green money willow seed processed in step (2) is soaked 6 days at clear water, be filtered dry, soak 24 hours by 400ppm Gibberellins solution, then with the wet sand mixing that the leaching of 400ppm gibberellin is mixed, load plastic storage case, be placed in 6 DEG C of refrigerator Stratificated treatment 35 days.Then chest is placed in 30 DEG C of constant incubator Stratificated treatment 50 days, moisturizing is ventilated, and keeps humidity about 70%.To be placed in-18 DEG C of low temperature refrigerators lamination 30 days.
3, drilling is germinateed
In late March, 2014, carries out drilling by gained seed after process, and seed furrow is apart from 25cm, dark 3cm, thickness of earth covering 2cm, and matrix is the mixture of red soil and vermiculite, and its weight ratio is 7:1.Red soil takes from the soil layer of upper soll layer 5 ~ below 10cm, breaks into pieces, after fully mixing in proportion, sprays 2 times with carbendazim, carry out soil disinfection with vermiculite, sows front water and irrigates, covered plastic booth.To spray water later every day moisturizing.
After planting about 30d, germination rate is 81.9%.

Claims (5)

1. a processing method for blue or green money willow seed, is characterized in that, comprise the following steps:
(1) the blue or green money willow seed of harvesting ripe, removes fruit wing, mechanically wear down seed coat;
(2) blue or green money willow seed step (1) processed crumples elutriation 6 ~ 8 times in the clear water adding washing agent, with the liquor potassic permanganate of 50ppm seed soaking sterilization 0.5 hour, cleans and dries;
(3) blue or green money willow seed clear water step (2) processed soaks 4 ~ 6 days, be filtered dry, 1 day is soaked by 400ppm Gibberellins solution, then the wet sand mixed is soaked as lamination matrix with 400ppm gibberellin, mixing, husky was 3:1 with the volume ratio of seed, loads antistaling box, in 2 ~ 6 DEG C of refrigerator laminations 30 ~ 35 days; Then sift out seed, then with the wet sand mixing that the leaching of 400ppm gibberellin is mixed, be placed in 20 ~ 30 DEG C of constant incubator laminations 40 ~ 50 days; And then with the wet sand mixing that the leaching of 400ppm gibberellin is mixed, to be placed in-2 ~-20 DEG C of low temperature refrigerators lamination 30 ~ 35 days.
2. the processing method of blue or green money willow seed according to claim 1, it is characterized in that, step mechanically grinds off 1/3 ~ 2/3 exosper in (1).
3. the processing method of blue or green money willow seed according to claim 1, it is characterized in that, the washing agent in step (2) is alkaline synthetic detergent.
4. the processing method of blue or green money willow seed according to claim 1, is characterized in that, in step (3), in Stratificated treatment process, often stir inspection, keeps seed moistening, notes mildew-resistant.
5. the processing method of blue or green money willow seed according to claim 1, is characterized in that, in step (3), prior to 4 DEG C of refrigerator laminations, is then placed in 20 DEG C of constant incubator laminations, and then is placed at lamination in-2 ~-20 DEG C of low temperature refrigerators.
CN201410771271.7A 2014-12-15 2014-12-15 A kind of processing method of blue or green money willow seed Withdrawn - After Issue CN104365220B (en)

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Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104782269A (en) * 2015-04-28 2015-07-22 商城县鑫林种养殖专业合作社 Cultivation method of cyclocarya paliurus seeds
CN104920483A (en) * 2015-05-29 2015-09-23 阜南县猛发工艺品有限公司 High-effective willow seed treatment agent
CN105123381A (en) * 2015-07-21 2015-12-09 广西龙胜立兵生物科技开发有限公司 Seedling culturing method for cyclocarya paliurus
CN105165578A (en) * 2015-08-26 2015-12-23 湖北思慧生物科技有限公司 Seedling method for cyclocarya paliurus seeds
CN105191540A (en) * 2015-09-25 2015-12-30 榕江平阳生态源开发有限责任公司 Cyclocarya paliurus seed soaking method
CN105900771A (en) * 2016-04-21 2016-08-31 剑河兴农林木种苗专业合作社 Cyclocarya paliurus propagating method
CN106069494A (en) * 2016-06-21 2016-11-09 资源县高山青钱柳种植专业合作社 A kind of method improving Cyclocarya paliurus Iljinskaja percentage of seedgermination
CN106068772A (en) * 2016-07-05 2016-11-09 江苏碧云天农林科技有限公司 A kind of method promoting Cyclocarya paliurus Iljinskaja seed Rapid Rooting to germinate
CN106105484A (en) * 2016-06-30 2016-11-16 南京林业大学 One breaks the seed dormant method of Cyclocarya paliurus Iljinskaja
CN106212159A (en) * 2016-07-28 2016-12-14 阜南县东奥工艺品有限公司 A kind of method for culturing seedlings of Salix babylonica L.
CN106211860A (en) * 2016-08-15 2016-12-14 黄平县源顺生态园有限公司 A kind of method improving Cyclocarya paliurus Iljinskaja percentage of seedgermination
CN106234103A (en) * 2016-07-28 2016-12-21 阜南县东奥工艺品有限公司 A kind of implantation methods of high-survival rate Salix babylonica L.
CN107371753A (en) * 2017-08-30 2017-11-24 贵州侗乡生态农业科技发展有限公司 A kind of breeding method of blue or green money willow seedling
CN108029428A (en) * 2017-12-15 2018-05-15 绩溪县万禾林业开发有限公司 A kind of method for culturing seedlings of higher survival rate blue or green money willow
CN109526446A (en) * 2018-12-20 2019-03-29 河西学院 A kind of low alternating temperature stratification overlay film direct sowing and seedling method of shiny-leaved yellowhorn seed
CN111096116A (en) * 2018-10-29 2020-05-05 广州中医药大学(广州中医药研究院) Method for treating evodia lepta seeds

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Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104782269A (en) * 2015-04-28 2015-07-22 商城县鑫林种养殖专业合作社 Cultivation method of cyclocarya paliurus seeds
CN104920483A (en) * 2015-05-29 2015-09-23 阜南县猛发工艺品有限公司 High-effective willow seed treatment agent
CN105123381B (en) * 2015-07-21 2017-09-05 广西龙胜立兵生物科技开发有限公司 A kind of method for culturing seedlings of blue or green money willow
CN105123381A (en) * 2015-07-21 2015-12-09 广西龙胜立兵生物科技开发有限公司 Seedling culturing method for cyclocarya paliurus
CN105165578A (en) * 2015-08-26 2015-12-23 湖北思慧生物科技有限公司 Seedling method for cyclocarya paliurus seeds
CN105191540A (en) * 2015-09-25 2015-12-30 榕江平阳生态源开发有限责任公司 Cyclocarya paliurus seed soaking method
CN105900771A (en) * 2016-04-21 2016-08-31 剑河兴农林木种苗专业合作社 Cyclocarya paliurus propagating method
CN106069494A (en) * 2016-06-21 2016-11-09 资源县高山青钱柳种植专业合作社 A kind of method improving Cyclocarya paliurus Iljinskaja percentage of seedgermination
CN106105484B (en) * 2016-06-30 2019-03-01 南京林业大学 A method of breaking blue or green money willow seed dormancy
CN106105484A (en) * 2016-06-30 2016-11-16 南京林业大学 One breaks the seed dormant method of Cyclocarya paliurus Iljinskaja
CN106068772A (en) * 2016-07-05 2016-11-09 江苏碧云天农林科技有限公司 A kind of method promoting Cyclocarya paliurus Iljinskaja seed Rapid Rooting to germinate
CN106212159A (en) * 2016-07-28 2016-12-14 阜南县东奥工艺品有限公司 A kind of method for culturing seedlings of Salix babylonica L.
CN106234103A (en) * 2016-07-28 2016-12-21 阜南县东奥工艺品有限公司 A kind of implantation methods of high-survival rate Salix babylonica L.
CN106211860A (en) * 2016-08-15 2016-12-14 黄平县源顺生态园有限公司 A kind of method improving Cyclocarya paliurus Iljinskaja percentage of seedgermination
CN107371753A (en) * 2017-08-30 2017-11-24 贵州侗乡生态农业科技发展有限公司 A kind of breeding method of blue or green money willow seedling
CN108029428A (en) * 2017-12-15 2018-05-15 绩溪县万禾林业开发有限公司 A kind of method for culturing seedlings of higher survival rate blue or green money willow
CN111096116A (en) * 2018-10-29 2020-05-05 广州中医药大学(广州中医药研究院) Method for treating evodia lepta seeds
CN111096116B (en) * 2018-10-29 2022-06-14 广州中医药大学(广州中医药研究院) Method for treating evodia lepta seeds
CN109526446A (en) * 2018-12-20 2019-03-29 河西学院 A kind of low alternating temperature stratification overlay film direct sowing and seedling method of shiny-leaved yellowhorn seed

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