CN103563575A - Storage method for increasing germination rate of michelia macclurei seeds - Google Patents
Storage method for increasing germination rate of michelia macclurei seeds Download PDFInfo
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- CN103563575A CN103563575A CN201310530447.5A CN201310530447A CN103563575A CN 103563575 A CN103563575 A CN 103563575A CN 201310530447 A CN201310530447 A CN 201310530447A CN 103563575 A CN103563575 A CN 103563575A
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Abstract
The invention discloses a storage method for increasing the germination rate of michelia macclurei seeds. The storage method comprises the steps as follows: ripe michelia macclurei cones are placed in a shade place for natural drying; the michelia macclurei cones are turned over frequently till the cones dehisce; seeds coated with seed coats are peeled off the cones, soaked in tap water for 1-12 hours and rubbed with wet sand according to a volume ratio of (1-5): (1-5); after the seed coats of the seeds are peeled off, the seeds are collected and placed in the shade place for natural drying; and the well processed seeds, drying sand and water are mixed uniformly according to a volume ratio of (1-10): (5-15): (1-3) and stored at a temperature of minus 20 DEG C to 25 DEG C after vacuum pumping is performed. According to the storage method, seed mildew cannot be caused easily, the germination rate of the michelia macclurei seeds after long-time storage can be increased to a large extent, and compared with a conventional storage method, the germination rate is increased by more than 50%; and the operation is simple, the cost is low, the promotion is easy, the problem that the germination rate of the michelia macclurei seeds after long-time storage is very low can be effectively solved, and the storage method has a wide application prospect.
Description
Technical field
The invention belongs to plant seed storage technique field, specifically, the present invention relates to improve the storage practice of Michelia macclurei percentage of seedgermination.
Background technology
Michelia macclurei (Michelia macclurei Dandy) has another name called michelia macclurei, belong to the evergreen megaphanerophyte of Magnoliaceae Michelia, the height of tree can reach 35m, the diameter of a cross-section of a tree trunk 1.3 meters above the ground can reach 1.2m, main natural distributed is in Guangdong and Guangxi intersection, and on the south Yi Daiji the Changjiang river, the Changjiang river, all there is extensive introducing and planting each provinces and regions, is that the important broad-leaved of one's native land of south China is precious with material and multifunctional efficient benefit seeds, there is the features such as strong adaptability, damage by disease and insect is few, growth is very fast, and form is perfectly straight, out-put is high, material is good.Michelia macclurei is to have the multiduty seeds of high value, suitablely does landscape tree, shade tree and street tree; Desirable fire prevention, improve the soil, water source self-restraint and ecoscape seeds; Also be precious fast-growing commerical tree species, its timber has gloss, structure is thin and even, texture is straight, rotproofness good, sapwood availability is high, have pungent smell can ant prevention erosion etc. feature, wood usage is extensive, is the high-quality timber for building construction, first-class furniture, timber floor, gun stock, balustrade etc.Michelia macclurei is also medicine food plant, and its leaf, bark, flower can extract the active ingredients such as plants essential oil.
In recent years, along with the continuous increase of society to precious timber demand, timber resources is day by day rare, and Michelia macclurei is as Precious Timber Species of one's native land, and the feature such as compare other indigenous tree speciess and have strong adaptability, grow and be very fast, has huge exploitation potentiality.The forest plantation planting range of Michelia macclurei constantly expands in recent years, and the cultivation of Michelia macclurei and seed selection research are also paid attention to gradually.
Yet the breeding of Michelia macclurei so far mainly relies on planting seed to grow seedlings, without report and the application example of cuttage and graft technology, Michelia macclurei plant tissue culture technique also has a small amount of report temporarily, and research is ripe not enough, does not drop in actual production and applies.For the germination rate of guaranteeing that Michelia macclurei seed is higher, current Michelia macclurei seed propagation seedling technology is to pluck fresh ripe Michelia macclurei cone, and processing is sowed after obtaining seed at once.Therefore, utilize seed to preserve Michelia macclurei high-quality germ plasm resource.
(ecological characteristic of fine tree species Michelia macclurei and the culture technique such as Chen Zhuohua, forest-science, 12 phases in 2011,212-223) with the refined equalization of Yao (research of michelia macclurei seedling growth test, Forestry in Guizhou Province science and technology, 2001,29(1): 46-48) adopt respectively moistening husky layering storage and Sha Zangzhi to sow the beginning of spring in next year, these methods are sowed and can be obtained good germination rate after preserving for the seed short period, if but take the said method storage long period, percentage of seedgermination reduces greatly.We test discovery, adopt the store method of above-mentioned routine to contain higher moisture, and seed is mouldy (mold of seed rate was directly proportional to the time) easily, and through 1 year above storage time, percentage of seedgermination was almost nil.And if seed is dried to preservation, thereby seed can dry out and loses germinating capacity.So Michelia macclurei seed storage technology is to be related to Michelia macclurei cultivation and seed selection research, and the major issue of actual production.Efficiently storage technique is preserved and is played an important role Michelia macclurei breeding, and Michelia macclurei is grown seedlings and cultivated to utilize plays an important role.
Summary of the invention
Based on this, in order to overcome the shortcoming and deficiency of above-mentioned prior art, the invention provides the storage practice that improves Michelia macclurei percentage of seedgermination.
To achieve these goals, the present invention has taked following technical scheme:
The storage practice that improves Michelia macclurei percentage of seedgermination, comprises the following steps:
(1), the In Shade natural air drying of ripe Michelia macclurei cone, often stir until cone cracking, kind of a seed for clothing parcel is peeled off to cone, being placed in running water soaked after 1～12 hour, rub 1～5:1～5 by volume with sand, after seed departs from kind of clothing, collect seed, shady and cool place is air-dry; The modulus of fineness of described sand is 1.6-2.2, and water content is 35-45%;
(2), seed that step (1) is handled well, mix 1～10:5～15:1～3 by volume with dry sand, water, vacuumize in-20～25 ℃ of preservations; The modulus of fineness of described sand is 1.6-2.2.
Therein in an embodiment, the volume ratio 4:8:1 of seed described in step (2), dry sand and water.This ratio is to take sand as basic, regulates moisture and seed addition, makes the humidity of sand moderate, can be too not dry, and also too much water is not deposited on storage sack bottom; Seed and sand are conducive to seed in wet husky complete encirclement after fully mixing, be unlikely to again sand too many.
In an embodiment, in step (2), seed is stored in 4 ℃ of refrigerators therein.Storage temperature is too low, and the moisture in wet sand can be in the state that freezes, and is not easy to allow seed in low temperature and has in the environment of certain humidity; Storage temperature is too high, easily produces mould.Therefore, 4 ℃ of the bests of storage temperature.
In an embodiment, described in step (1), the volume ratio of seed and sand is 1:1 therein.Collect after seed, the ratio of employing 1:1 and wet sand are washed by rubbing with the hands and are removed in kind of clothing process, and effect is relatively good.Seed specific humidity sand is many, washes by rubbing with the hands in process and easily causes seed and seed friction, causes seed impaired; Seed specific humidity is husky few, washes that to remove the speed of seed slow by rubbing with the hands.
In an embodiment, in step (1), the seed of described kind of clothing parcel is placed in to running water and soaks 3～6h therein.Test discovery, the kind clothing of ripe seed enough allows kind of a clothing immersion swell in this time period of 1-12h, then easily washes by rubbing with the hands and removes kind of a clothing with wet sand, if the too short effect that do not reach of time, the oversize meeting of seed soak time in water affect percentage of seedgermination.Soak the sand that dries after 3～6h and wash that to remove the effect of kind of clothing best by rubbing with the hands.
Compared with prior art, the present invention has following beneficial effect:
1. Michelia macclurei seed storage method of the present invention has added appropriate moisture in sand, can allow seed in storage, keep certain water content, thereby can improve largely the percentage of seedgermination of Michelia macclurei seed after long-time storage, than the percentage of seedgermination of conventional storage practice, improve more than 50%; And storage practice of the present invention in suitable temperature range, is difficult for causing that seed goes mouldy by treated seed storage;
2. Michelia macclurei seed storage method of the present invention does not need loaded down with trivial details treatment step and special storage facilities, simple to operate, with low cost, easily promote, can effectively solve the long-time storage of Michelia macclurei seed and cause the problem that germination rate is very low, can be and effectively alleviate from now on the breeding situation that supply falls short of demand, have broad application prospects.
Embodiment
Below the present invention is further elaborated by specific embodiment, and its object is only to understand better content of the present invention, and protection scope of the present invention comprises and is not limited in the cited case.
The Michelia macclurei cone using in following examples is plucked the good Michelia macclurei individual plant family filial generation from Rong County, Guangxi, and seed is from same strain elite stand, and elite stand is numbered: RITF-RX05(HLN94).
Embodiment 1 improves the storage practice of Michelia macclurei percentage of seedgermination
Step is as follows:
1, the ripe Michelia macclurei cone of gathering is packed in breathable nylon bag, be placed in shady and cool ventilation place natural air drying, often stir Nylon Bag until cone cracking exposes red seed of planting clothing parcel, redness is planted to the seed of clothing parcel and peel off cone, the seed of collection is placed in to running water and soaks 3～6h, pull out the seed that soaks and wet sand by volume 1:1 rub, until the Michelia macclurei seed of sepia or black, depart from after kind of clothing, collect seed and clean, shady and cool place is air-dry.
2, the Michelia macclurei seed of handling well, mixes according to 4:8:1 volume ratio with dry sand, water, with sealed bag, vacuumizes and is stored in 4 ℃ of refrigerators.
Adopt the condition of embodiment 1; 100g seed is peeled off kind of clothing need to approximately 20 minutes; seed does not have breakage; after seed is air-dry, done repeated test 3 times, each 150 Michelia macclurei seeds of repeated using carry out storage test, take out seed and sow after 1 year; repeat for 3 times to be respectively 0,5,8 with the seed number of mould; sow after 2 months the seed seedling of sprouting is carried out to statistical analysis, germination rate is respectively 74.3%, 67.6%, 73.1%, and the seed height of seedling of sprouting is consistent.
Embodiment 2 improves the storage practice of Michelia macclurei percentage of seedgermination
Except Michelia macclurei seed in step 2, mix according to 8:8:1 volume ratio with dry sand, water, with sealed bag, vacuumize and be stored in 10 ℃ of refrigerator China and foreign countries, other operations are all identical with embodiment 1.
Adopt the condition of embodiment 2; 100g seed is peeled off kind of clothing need to approximately 20 minutes; seed does not have breakage; after seed is air-dry, done repeated test 3 times, each 150 Michelia macclurei seeds of repeated using carry out storage test, take out seed and sow after 1 year; repeat for 3 times to be respectively 67,52,83 with the seed number of mould; sow after 2 months the seed seedling of sprouting is carried out to statistical analysis, germination rate is respectively 52.3%, 60.7%, 41.2%, and the seed height of seedling of sprouting is unanimous on the whole.
Embodiment 3 improves the storage practice of Michelia macclurei percentage of seedgermination
Except Michelia macclurei seed in step 2, mix according to 4:12:1.5 volume ratio with dry sand, water, with sealed bag, vacuumize and be stored in-20 ℃ of refrigerators, other operations are all identical with embodiment 1.
Adopt the condition of embodiment 3; 100g seed is peeled off kind of clothing need to approximately 20 minutes; seed does not have breakage; after seed is air-dry, done repeated test 3 times, each 150 Michelia macclurei seeds of repeated using carry out storage test, take out seed and sow after 1 year; the seed not going mouldy in repeating for 3 times; sow after 2 months the seed seedling of sprouting is carried out to statistical analysis, germination rate is respectively 59.2%, 54.3%, 55.0%, and the seed height of seedling of sprouting is unanimous on the whole.The kind shell of the seed that strip off is not germinateed, the embryo dehydration of the inside seriously causes shrivelled, and this explanation reserve temperature is too low, makes embryo dehydration serious, thereby has affected germination rate.
Embodiment 4 improves the storage practice of Michelia macclurei percentage of seedgermination
Except the seed of collection being placed in to running water in step 1, soak 1～2h, outside pulling the seed that soaks and wet sand out 1:4 rubbing by volume, other operations are all identical with embodiment 1.
Adopt the condition of embodiment 4; 100g seed is peeled off kind of clothing need to approximately 60 minutes; seed does not have breakage; after seed is air-dry, done repeated test 3 times, each 150 Michelia macclurei seeds of repeated using carry out storage test, take out seed and sow after 1 year; repeat for 3 times to be respectively 0,3,0 with the seed number of mould; sow after 2 months the seed seedling of sprouting is carried out to statistical analysis, germination rate is respectively 72.3%, 75.6%, 68.0%, and the seed height of seedling of sprouting is unanimous on the whole.
Embodiment 5 improves the storage practice of Michelia macclurei percentage of seedgermination
Except the seed of collection being placed in to running water in step 1, soak 10～12h, outside pulling the seed that soaks and wet sand out 4:1 rubbing by volume, other operations are all identical with embodiment 1.
Adopt the condition of embodiment 5; 100g seed is peeled off kind of clothing need to approximately 20 minutes; seed minority has breakage; after seed is air-dry, done repeated test 3 times, each 150 Michelia macclurei seeds of repeated using carry out storage test, take out seed and sow after 1 year; repeat for 3 times to be respectively 6,7,4 with the seed number of mould; sow after 2 months the seed seedling of sprouting is carried out to statistical analysis, germination rate is respectively 65.5%, 62.8%, 70.4%, and the seed height of seedling of sprouting is unanimous on the whole.
The comparison of the inventive method and existing conventional method
The inventive method and traditional Michelia macclurei seed storage method, and Michelia macclurei seed to adopt be the percentage of seedgermination result of the test comparison of broadcasting method, as table 1.
Table 1
As can be seen from Table 1, although adopt storage practice of the present invention to adopt than Michelia macclurei seed that to broadcast germination rate lower slightly, to compare with traditional conventional storage practice, seed is not easy mouldy, and in storage, after 1 year, can also keep 72.8% percentage of seedgermination, germination rate is high.
The above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (5)
1. improve the storage practice of Michelia macclurei percentage of seedgermination, it is characterized in that, comprise the following steps:
(1), the In Shade natural air drying of ripe Michelia macclurei cone, often stir until cone cracking, kind of a seed for clothing parcel is peeled off to cone, being placed in running water soaked after 1～12 hour, rub 1～5:1～5 by volume with sand, after seed departs from kind of clothing, collect seed, shady and cool place is air-dry; The modulus of fineness of described sand is 1.6-2.2, and water content is 35-45%;
(2), seed that step (1) is handled well, mix 1～10:5～15:1～3 by volume with dry sand, water, vacuumize in-20～25 ℃ of preservations; The modulus of fineness of described sand is 1.6-2.2.
2. the storage practice of raising Michelia macclurei percentage of seedgermination according to claim 1, is characterized in that, the volume ratio 4:8:1 of seed described in step (2), dry sand and water.
3. the storage practice of raising Michelia macclurei percentage of seedgermination according to claim 1, is characterized in that, in step (2), seed is stored in 4 ℃ of refrigerators.
4. according to the storage practice of the raising Michelia macclurei percentage of seedgermination described in claim 1-3, it is characterized in that, described in step (1), the volume ratio of seed and sand is 1:1.
5. according to the storage practice of the raising Michelia macclurei percentage of seedgermination described in claim 1-3 any one, it is characterized in that, in step (1), the seed of described kind of clothing parcel is placed in to running water and soaks 3～6h.
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| CN104620717A (en) * | 2015-02-10 | 2015-05-20 | 广西壮族自治区林业科学研究院 | Preparation method of seedling seeds of michelia macclurei |
| CN105532116A (en) * | 2016-03-04 | 2016-05-04 | 中国林业科学研究院资源昆虫研究所 | Gnetum pendulum seed treatment method |
| CN110366962A (en) * | 2019-07-26 | 2019-10-25 | 江苏省农业科学院 | A kind of store method of radish germ plasm resource |
| CN111642170A (en) * | 2020-07-10 | 2020-09-11 | 四川省林业科学研究院 | Method for preserving seed of Acer catalpa |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104620717A (en) * | 2015-02-10 | 2015-05-20 | 广西壮族自治区林业科学研究院 | Preparation method of seedling seeds of michelia macclurei |
| CN104620717B (en) * | 2015-02-10 | 2016-09-21 | 广西壮族自治区林业科学研究院 | A kind of modulator approach of Michelia macclurei nursery seed |
| CN105532116A (en) * | 2016-03-04 | 2016-05-04 | 中国林业科学研究院资源昆虫研究所 | Gnetum pendulum seed treatment method |
| CN110366962A (en) * | 2019-07-26 | 2019-10-25 | 江苏省农业科学院 | A kind of store method of radish germ plasm resource |
| CN111642170A (en) * | 2020-07-10 | 2020-09-11 | 四川省林业科学研究院 | Method for preserving seed of Acer catalpa |
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