CN104230519A - Method for preparing culture medium for edible mushroom - Google Patents
Method for preparing culture medium for edible mushroom Download PDFInfo
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- CN104230519A CN104230519A CN201410376225.7A CN201410376225A CN104230519A CN 104230519 A CN104230519 A CN 104230519A CN 201410376225 A CN201410376225 A CN 201410376225A CN 104230519 A CN104230519 A CN 104230519A
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Abstract
The invention relates to the technical field of cultivation of edible mushrooms and particularly relates to a method for preparing a culture medium for an edible mushroom. The method mainly comprises the following steps: processing walnut shells, firstly pulverizing the walnut shells into particles of which the diameter is less than 8mm by virtue of a high-speed pulverizer, placing the pulverized walnut shells into a high-pressure reactor equipped with ultrasonic waves and adding water of which the mass is 2.5-5.5 times of mass of the walnut shells, introducing nitrogen so that the pressure inside the reactor is 1.2MPa-15.5MPa, starting an ultrasonic generator, carrying out ultrasonic radiation for 5-45 minutes at room temperature, dehydrating and airing for later use. According to the method disclosed by the invention, by adopting a high-pressure ultrasonic irradiation method, the dissolution rate of tannin having inhibiting effect on the growth of the mushroom in the walnut shells is accelerated, the soaking time is reduced, the working efficiency is improved; the yield of the edible mushroom is increased by 18%-35% than the yield of the edible mushroom cultured by virtue of the traditional soaking medium, the production cost is significantly reduced and the market competitiveness is improved.
Description
Technical field
The present invention relates to fungus growing technique field, be specifically related to a kind of preparation method of edible fungus culture medium.
Background technology
Walnut, deciduous tree, imparipinnate leaf, leaflet is oval, and drupe is spherical, and exocarp is level and smooth, and endocarp is hard, has wrinkle, in brain shape.Belong to Spermatophyta, timber is tough and tensile, can make implements, and kernel can be eaten, and can extract oil, and also can be used as medicine, not only delicious, and nutritive value is very high, is described as " long live ", " Longevity ".Nut-shell is endocarp, it is a kind of particulate state crossed through processings such as degreasing, fragmentation, screenings, surface many micropores, the good filtrate of adsorption effect are also natural tumbling material and polish abrasive, do abrasive material can not destroy the advantages such as workpiece surface, polishing effect are good with it.Its stable chemical nature, in acid, alkali, water, meltage is very little, and the loss of nut-shell in hydrochloric acid soln is 4.99%, and the wastage rate in sodium hydroxide solution are 3.8%, and can not cause water quality deterioration phenomenon.
The purposes of current nut-shell is mainly processed as artwork, fires gac or grind and make fertilizer etc.Nut-shell is applied to edible mushrooms field, the research emphasis of this area always, but owing to containing vegatable tannin in nut-shell, and tannin has bacteriostatic activity on edible fungus culturing, suppress the normal growth of edible fungus mycelium, thus limit nut-shell and apply on edible fungus culturing.Denomination of invention is " method taking nut-shell as substrate culture edible mushrooms ", publication number is that to disclose a kind of take nut-shell as the method for substrate culture edible mushrooms to the patent of invention of CN100999418A, nut-shell room temperature is soaked in water 24 ~ 72 hours, to reduce tannin content; Denomination of invention is " a kind of preparation method of matrix of culturing edible fungus ", publication number is that the patent of invention of CN103922841A discloses " a kind of preparation method of matrix of culturing edible fungus ", it is with being soaked by nut-shell in vegetation buck 24 ~ 48 hours that this invention reduces the method for tannin content, but the method that infusion method reduces nut-shell tannin content all exists length consuming time, soaks the defect destroyed to edible mushrooms beneficiating ingredient in nut-shell for a long time.
Ultrasonic frequency is high, and power is large, can cause cavatition in a liquid, the density of liquid can be caused to change, tension when making liquid, time and pressurized.When liquid tension, liquid will rupture, and produces the little hole of some near vacuum.In liquid pressurized process, hole is collapsed, along with the huge sudden change of pressure by Hair Fixer life.All show from theory and practice, under the Ultrasonic Radiation effect of certain frequency, solvent-swollen is carried out to nut-shell, has significant effect for some chemical bond in loose nut-shell macromolecular network structure and fracture nut-shell macromolecular network structure, there is obvious reaming effect.But the nut-shell of Ultrasonic Radiation is applied to the matrix of cultivating edible mushrooms and yet there are no relevant report.
Summary of the invention
For above-mentioned technological deficiency, the invention provides a kind of preparation method of edible fungus culture medium, culture medium prepared by the present invention is more loose, be convenient to the absorption of edible mushrooms to nutritive substance, utilize the quick growth of edible mushrooms, output, compared with conventional immersion matrix, increases production 18% ~ 35%, greatly reduce production cost, improve the market competitiveness.
Technical solutions according to the invention are realized by following measures:
A preparation method for the matrix of culturing edible fungus, comprises the steps:
(1) nut-shell process, first with high speed disintegrator, walnut shell flour is broken into the particle that diameter is below 8mm, then the nut-shell of pulverizing is put into and ultrasonic high pressure reactor is housed and adds water, the quality of water is 2.5 ~ 5.5 times of nut-shell quality, pour nitrogen to make still internal pressure for 1.2MPa ~ 15.5MPa, open ultrasonic generator, ultrasonic radiation 5-45min under room temperature condition, anhydrates air-dry stand-by.
(2) edible fungus culture medium is made up of by mass percentage following raw material: nut-shell 57.0% ~ 80.0%, pine sawdust 10.0% ~ 15.0%, wheat bran 4.0% ~ 10.0%, dregs of beans: 3.0% ~ 8.0%, Semen Maydis powder: 3.0% ~ 6.0%, plaster stone 1.0% ~ 4.0%.
(3) step (2) materials in percentage by mass mixes pack i.e. obtained edible fungi substrate through mixing machine.
Described hyperacoustic frequency is 15Hz ~ 48Hz.
Described hyperacoustic frequency is 20Hz ~ 24Hz.
The described ultrasonic radiation time is 20min ~ 35Hz.
Described edible fungus culture medium is made up of by mass percentage following raw material: step (1) nut-shell 67.5.0%, pine sawdust 13.5%, wheat bran 5.5%, dregs of beans: 6.5%, Semen Maydis powder: 5.0%, plaster stone 2.0%.
Herbicidal composition of the present invention beneficial effect be: the present invention adopts high pressure ultrasonic radiation method first, accelerates speed fungus grown being had in nut-shell to the stripping of inhibiting material tannin, reduces soak time, improve working efficiency; Simultaneously, the huge sudden change of ultrasonic pressure, make that hard capsomere becomes loose, particle diameter is little, each component mixing is more even, and matrix is more loose, be convenient to the absorption of edible mushrooms to nutrition, utilize the quick growth of edible mushrooms, output, compared with conventional immersion matrix, increases production 18% ~ 35%, greatly reduce production cost, improve the market competitiveness.It is tender and crisp that the edible fungi nutrition that culture medium of the present invention grows enriches mouthfeel, without the strange taste being difficult to describe.
Embodiment
Embodiment 1
The present invention's high speed disintegrator used is the medium-sized high speed disintegrator of YS-10 type that Jiangyin City's health and Machinery Manufacturing Co., Ltd. produce; It is the XO-GY500 type ultrasonic high pressure reactor that Nanjing Xian Ou instrument manufacturing company limited produces that ultrasonic wave sends out high pressure vessel; Mixing machine is the 9HZTP-300 drum type brake ribbon mixer that the production of electromechanical company limited is moulded in Luoyang.
A preparation method for the matrix of cultivating white mushroom, comprises the steps:
(1) nut-shell is processed, walnut shell flour is broken into the particle that diameter is below 8mm, pulverize nut-shell put into container that ultrasonic generator is housed and add water, ultrasonic frequency is 15 Hz, room temperature ultrasonic radiation 5min, still internal pressure is 1.2MPa, and the quality of water is 5.5 times of nut-shell quality, anhydrates air-dry stand-by;
(2) edible fungus culture medium is made up of by mass percentage following raw material: step (1) nut-shell 79.0%, pine sawdust 10.0%, wheat bran 4.0%, dregs of beans 3.0%, Semen Maydis powder 3.0%, plaster stone 1.0%.
(3) step (2) materials in percentage by mass mixes pack i.e. obtained edible fungi substrate through mixing machine.
Embodiment 2
A preparation method for the matrix of mushroom culture, comprises the steps:
(1) nut-shell is processed, walnut shell flour is broken into the particle that diameter is below 6mm, pulverize nut-shell put into container that ultrasonic generator is housed and add water, ultrasonic frequency is 18 Hz, room temperature ultrasonic radiation 20min, still internal pressure is 2.5MPa, and the quality of water is 5.0 times of nut-shell quality, anhydrates air-dry stand-by;
(2) edible fungus culture medium is made up of by mass percentage following raw material: step (1) nut-shell 70.0%, pine sawdust 12.0%, wheat bran 6.0%, dregs of beans 5.0%, Semen Maydis powder 5.0%, plaster stone 2.0%.
(3) step (2) materials in percentage by mass mixes pack i.e. obtained edible fungi substrate through mixing machine.
Embodiment 3
Cultivate a preparation method for the matrix of white fungus, comprise the steps:
(1) nut-shell is processed, walnut shell flour is broken into the particle that diameter is below 4mm, pulverize nut-shell put into container that ultrasonic generator is housed and add water, ultrasonic frequency is 20 Hz, room temperature ultrasonic radiation 25min, still internal pressure is 3.8MPa, and the quality of water is 4.5 times of nut-shell quality, anhydrates air-dry stand-by;
(2) edible fungus culture medium is made up of by mass percentage following raw material: step (1) nut-shell 72.0%, pine sawdust 11.0%, wheat bran 5.0%, dregs of beans 5.0%, Semen Maydis powder 5.0%, plaster stone 2.0%.
(3) step (2) materials in percentage by mass mixes pack i.e. obtained edible fungi substrate through mixing machine.
Embodiment 4
A preparation method for the matrix of fungus cultivation, comprises the steps:
(1) nut-shell is processed, walnut shell flour is broken into the particle that diameter is below 3.5mm, pulverize nut-shell put into container that ultrasonic generator is housed and add water, ultrasonic frequency is 22 Hz, room temperature ultrasonic radiation 30min, still internal pressure is 8.2MPa, and the quality of water is 3.8 times of nut-shell quality, anhydrates air-dry stand-by;
(2) edible fungus culture medium is made up of by mass percentage following raw material: step (1) nut-shell 57.0%, pine sawdust 15.0%, wheat bran 10.0%, dregs of beans 8.0%, Semen Maydis powder 6.0%, plaster stone 4.0%.
(3) step (2) materials in percentage by mass mixes pack i.e. obtained edible fungi substrate through mixing machine.
Embodiment 5
Cultivate a preparation method for the matrix of dried mushroom, comprise the steps:
(1) nut-shell is processed, walnut shell flour is broken into the particle that diameter is below 3.2mm, pulverize nut-shell put into container that ultrasonic generator is housed and add water, ultrasonic frequency is 24 Hz, room temperature ultrasonic radiation 35min, still internal pressure is 12MPa, and the quality of water is 2.5 times of nut-shell quality, anhydrates air-dry stand-by;
(2) edible fungus culture medium is made up of by mass percentage following raw material: step (1) nut-shell 67.5%, pine sawdust 13.5%, wheat bran 5.5%, dregs of beans 6.5%, Semen Maydis powder 5.0%, plaster stone 2.0%.
(3) step (2) materials in percentage by mass mixes pack i.e. obtained edible fungi substrate through mixing machine.
Embodiment 6
Cultivate a preparation method for the matrix of Hericium erinaceus (Bull. Ex Fr.) Pers., comprise the steps:
(1) nut-shell is processed, walnut shell flour is broken into the particle that diameter is below 1.0mm, pulverize nut-shell put into container that ultrasonic generator is housed and add water, ultrasonic frequency is 48Hz, room temperature ultrasonic radiation 16min, still internal pressure is 10MPa, and the quality of water is 1.9 times of nut-shell quality, anhydrates air-dry stand-by;
(2) edible fungus culture medium is made up of by mass percentage following raw material: step (1) nut-shell 65.0%, pine sawdust 13.0%, wheat bran 7.0%, dregs of beans 6.0%, Semen Maydis powder 6.0%, plaster stone 3.0%.
(3) step (2) materials in percentage by mass mixes pack i.e. obtained edible fungi substrate through mixing machine.
Embodiment 7
Cultivate a preparation method for the matrix of dictyophora phalloidea, comprise the steps:
(1) nut-shell is processed, walnut shell flour is broken into the particle that diameter is below 1.5mm, pulverize nut-shell put into container that ultrasonic generator is housed and add water, ultrasonic frequency is 45Hz, room temperature ultrasonic radiation 45min, still internal pressure is 15.5MPa, and the quality of water is 2.6 times of nut-shell quality, anhydrates air-dry stand-by;
(2) edible fungus culture medium is made up of by mass percentage following raw material: step (1) nut-shell 68.0%, pine sawdust 12.0%, wheat bran 6.0%, dregs of beans 5.0%, Semen Maydis powder 6.0%, plaster stone 3.0%.
(3) step (2) materials in percentage by mass mixes pack i.e. obtained edible fungi substrate through mixing machine.
More than show and describe ultimate principle of the present invention, principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (5)
1. a preparation method for the matrix of culturing edible fungus, is characterized in that comprising the steps:
(1) nut-shell process, first with high speed disintegrator, walnut shell flour is broken into the particle that diameter is below 8mm, then the nut-shell of pulverizing is put into and ultrasonic high pressure reactor is housed and adds water, the quality of water is 2.5 ~ 5.5 times of nut-shell quality, pour nitrogen to make still internal pressure for 1.2MPa ~ 15.5MPa, open ultrasonic generator, ultrasonic radiation 5-45min under room temperature condition, anhydrates air-dry stand-by;
(2) edible fungus culture medium is made up of by mass percentage following raw material, mix through mixing machine: step (1) nut-shell 57.0% ~ 80.0%, pine sawdust 10.0% ~ 15.0%, wheat bran 4.0% ~ 10.0%, dregs of beans: 3.0% ~ 8.0%, Semen Maydis powder: 3.0% ~ 6.0%, plaster stone 1.0% ~ 4.0%;
(3) step (2) materials in percentage by mass mixes pack i.e. obtained edible fungi substrate through mixing machine.
2. the preparation method of the matrix of a kind of culturing edible fungus according to claim 1, is characterized in that: described hyperacoustic frequency is 15Hz ~ 48Hz.
3. the preparation method of the matrix of a kind of culturing edible fungus according to claim 1, is characterized in that: described hyperacoustic frequency is 20Hz ~ 24Hz.
4. the preparation method of the matrix of a kind of culturing edible fungus according to claim 1, is characterized in that: the described ultrasonic radiation time is 20min ~ 35Hz.
5. the preparation method of the matrix of a kind of culturing edible fungus according to claim 1, it is characterized in that: described edible fungus culture medium is made up of by mass percentage following raw material: nut-shell 67.5.0%, pine sawdust 13.5%, wheat bran 5.5%, dregs of beans: 6.5%, Semen Maydis powder: 5.0%, plaster stone 2.0%.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105924254A (en) * | 2016-06-22 | 2016-09-07 | 天津橙色味道食品科技有限公司 | Culture medium composition taking walnut shells and bagasse as main materials for edible fungi and preparation method |
| CN106116855A (en) * | 2016-06-22 | 2016-11-16 | 天津橙色味道食品科技有限公司 | Make full use of sucrose and extract edible fungus culture medium and the preparation method of residue |
| CN106116853A (en) * | 2016-06-22 | 2016-11-16 | 天津橙色味道食品科技有限公司 | Agricultural breeding edible fungus culture medium and preparation method thereof |
| CN106116725A (en) * | 2016-06-22 | 2016-11-16 | 天津橙色味道食品科技有限公司 | Edible fungi substrate composition and preparation method thereof |
| CN106978187A (en) * | 2017-03-29 | 2017-07-25 | 沂南县迎辉农业开发有限公司 | A kind of preparation method of plant source base soil conditioner |
| CN110637680A (en) * | 2018-06-27 | 2020-01-03 | 贵州金蟾大山生物科技有限责任公司 | Production method of Dictyophora rubrovalvata mildew-removing high-germination-rate cultivation stock |
| CN113501733A (en) * | 2021-08-09 | 2021-10-15 | 安徽省司尔特肥业股份有限公司 | Special functional bio-organic fertilizer for hickory and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106978187A (en) * | 2017-03-29 | 2017-07-25 | 沂南县迎辉农业开发有限公司 | A kind of preparation method of plant source base soil conditioner |
| CN110637680A (en) * | 2018-06-27 | 2020-01-03 | 贵州金蟾大山生物科技有限责任公司 | Production method of Dictyophora rubrovalvata mildew-removing high-germination-rate cultivation stock |
| CN110637680B (en) * | 2018-06-27 | 2023-08-18 | 贵州金蟾大山生物科技有限责任公司 | Production method of Dictyophora rubrovalvata mildew-removed and high-germination-rate cultivation stock |
| CN113501733A (en) * | 2021-08-09 | 2021-10-15 | 安徽省司尔特肥业股份有限公司 | Special functional bio-organic fertilizer for hickory and preparation method thereof |
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Application publication date: 20141224 |