CN104223063A - Freeze-dried dendrobium officinale powder and preparation method thereof - Google Patents
Freeze-dried dendrobium officinale powder and preparation method thereof Download PDFInfo
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Abstract
The invention discloses freeze-dried dendrobium officinale powder and a preparation method thereof. The method comprises the steps of (1) cleaning fresh picked dendrobium officinale with water, drying, and cutting the dendrobium officinale into sections which are 2-4cm long; (2) putting the dried dendrobium officinale into a juicing machine, adding pure water according to a ratio of material to liquid being 1g:5ml and juicing; (3) freezing the dendrobium officinale juice at the temperature of -20 DEG C for one night; (4) pre-cooling vacuum freeze-drying equipment at the temperature of -56 DEG C for half an hour; and (5) putting a frozen dendrobium officinale product into the pre-cooled vacuum freeze-drying equipment, and performing vacuum freeze-drying treatment under conditions that the temperature is -56 DEG C and the vacuum degree is 7-9Pa for 18-30 hours so as to obtain the freeze-dried dendrobium officinale powder. The method is simple, is easy to operate and is low in production cost; the obtained freeze-dried dendrobium officinale powder product is loose, is rich in dendrobium officinale polysaccharide, does not contain fibers and is high in dissolvability; the utilization rate of the officinale polysaccharide is high; furthermore, the original state of dendrobium officinale is retained, and the freeze-dried dendrobium officinale powder is light green, has a fresh taste and medicinal effects and can be stored and transported under normal temperature; the retention period is effectively prolonged.
Description
Technical field
The present invention relates to food processing field, particularly, relate to dendrobium candidum freeze-dried powder and preparation method thereof.
Background technology
Dendrobium candidum (Dendrobium officinale Kimura et Migo) is the orchid family (Orchidaceae) Dendrobium (Dendrobium) herbaceos perennial, be China's tradition rare traditional Chinese medicine, there is reinforcing stomach reg fluid, nourishing Yin and clearing heat, the unique effects such as moisten the lung and relieve the cough.In China's traditional Chinese medicine material, the stem of noble dendrobium appears in the Classic of Mountains and Rivers the earliest; As far back as Qin Han dynasty, Shennong's Herbal just records dendrobium candidum, and " taste is sweet, flat.In main wound, except numbness, lower gas, tonifying five zang organs consumptive disease, frail, reinforcing yin essence.Clothes of a specified duration, thick stomach, make light of one's life by commiting suicide, prolong life ".First of dendrobium candidum is classified as by " Taoist Scriptures " before more than 1000 year " Chinese nine immortal grass "; Li Shizhen (1518-1593 A.D.) evaluates dendrobium candidum " reinforcing yin essence benefit is smart, thick stomach, never sufficient in benefit, flat stomach Qi, longue meat, and intelligence development, except frightened, made light of one's life by commiting suicide and prolonged life " in Compendium of Material Medica.Among the peoplely be called the celestial grass of help, international medicinal plant circle is called " medicine circle giant panda ".2010 the version Pharmacopoeia of the People's Republic of China (one) iron sheet stone solution is separated from Shi Xiezhong, simply single-row, taste is sweet, is slightly cold.Return stomach, kidney channel.There is reinforcing stomach reg fluid, nourishing Yin and clearing heat effect.Hinder body fluid deficiency for the moon, dry polydipsia, deficiency of stomach-Yin, food is few retches, and abnormal heat is not moved back after being ill, fire excess from yin deficiency, and hectic fever due to yin labor heat, order is secretly failed to understand, muscles and bones impotence is soft.Scientific research shows, containing dendrobium polysaccharide, dendrobine, acids, sesquiterpenoids, steroid, some beneficiating ingredients such as amino acid and various trace elements in the stem of noble dendrobium, can improve human body body's immunity.Modern pharmacological research proves, dendrobium candidum has develop immunitypty, eliminates tumour, suppresses the effects such as cancer, all has significant curative effect to pharyngolaryngitis, gastrointestinal disease, cataract, angiocardiopathy, diabetes, tumour.In addition, in Chinese traditional medicine, the stem of noble dendrobium be also used for the treatment of child clearing heat and detoxicating, prevent the symptoms such as flu.
Because dendrobium candidum has good medical value and health value, people are increasing to its demand, but the iron-sheet dendrobe fresh product holding time is shorter, and though drying can extend the holding time afterwards, but organoleptic indicator is deteriorated, the particularly important is, effective physiologically active active component is also influenced, causes its effect to decline.
Thus, being badly in need of at present exploitation one can preserve for a long time, and organoleptic indicator is good, and the processing preparation technology of the effective dendrobium candidum product that the activity of physiologically active ingredient is good.
Summary of the invention
The present invention is intended at least to solve one of technical problem existed in prior art.For this reason, it is good that one object of the present invention is that proposition one can prepare organoleptic indicator, and the activity of effective physiologically active ingredient is good, the processing technology of dendrobium candidum product with long preservation period.
According to an aspect of the present invention, the invention provides a kind of method preparing dendrobium candidum freeze-dried powder.According to embodiments of the invention, the method comprises:
(1) fresh dendrobium candidum of gathering is cleaned, drained, be cut into the section that 2-4cm is long, for subsequent use;
(2) the dendrobium candidum section drained is placed in juice extractor, adds pure water with the ratio of solid-liquid ratio 1g:5ml and squeeze the juice;
(3) the dendrobium candidum juice obtained in step (2) is carried out-20 DEG C of freeze overnight, for subsequent use;
(4) vacuum freeze is carried out-56 DEG C of precooling half an hour; And
(5) the juice refrigerated product of dendrobium candidum obtained in step (3) are placed in the vacuum freeze through precooling treatment, in-56 DEG C, carry out vacuum freeze drying process 18-30 hour, to obtain dendrobium candidum freeze-dried powder under the condition of vacuum 7-9Pa.
Inventor is surprised to find, the method preparing dendrobium candidum freeze-dried powder of the present invention, equipment needed thereby is few, simple to operation, production cost is low, and the dendrobium candidum freeze-dried powder product utilizing method of the present invention to prepare loosens, and remains the reset condition of dendrobium candidum, light green color and luster, fresh taste and pharmacological effect, can normal temperature storage and transport, effectively extend storage life.Further, the dendrobium candidum freeze-dried powder of acquisition is rich in Dendrobium officinale polysaccharide, not fibre-bearing, and solubility is good, direct-edible or be used as batching and stew edible or make tea and drink.
In addition, following additional technical characteristic can also be had according to the method preparing dendrobium candidum freeze-dried powder of the embodiment of the present invention:
According to embodiments of the invention, described juice extractor is nine positive JYL-C010 cooking machines.This juice extractor can automatic fitration when squeezing the juice, the materials such as elimination fiber, thus when utilizing this juice extractor to squeeze the juice, the not fibre-bearing prepared by method of the present invention, direct-edible or be used as batching and stew edible or make tea and drink.
According to embodiments of the invention, at-20 DEG C, the dendrobium candidum juice obtained in step (2) is carried out freeze overnight.Thereby, it is possible to obtain the juice refrigerated product of the dendrobium candidum not damaging fresh taste and pharmacological effect efficiently, thus be conducive to carrying out follow-up vacuum freeze drying process.
According to embodiments of the invention, described vacuum freeze is FD-1A-50 type vacuum freeze drier, and preferred Shanghai produces than bright Instrument Ltd..This vacuum freeze-drying system equipment is the equipment being exclusively used in food processing, and its temperature is constant temperature-56 DEG C, farthest can retain the pharmacological effect of dendrobium candidum.
According to embodiments of the invention, carry out described vacuum freeze drying process 24 hours.Thus, the dendrobium candidum freeze-dried powder of acquisition can keep fresh taste and the pharmacological effect of dendrobium candidum, can normal temperature storage and transport, long shelf-life.
According to embodiments of the invention, method of the present invention comprises further: the dendrobium candidum freeze-dried powder that step (5) obtains is carried out vacuum packaging process by (6).Thus, the shelf-life of dendrobium candidum freeze-dried powder can effectively increase, and is convenient to normal temperature storage and transport.According to a concrete example of the present invention, food vacuum compressed bag is utilized to carry out described vacuum packaging process.Thus, convenient, fast, be convenient to normal temperature storage and the transport of dendrobium candidum freeze-dried powder.
According to another aspect of the invention, present invention also offers a kind of dendrobium candidum freeze-dried powder.According to embodiments of the invention, this dendrobium candidum freeze-dried powder is prepared by foregoing method.Inventor is surprised to find, and dendrobium candidum freeze-dried powder of the present invention is rich in Dendrobium officinale polysaccharide, not fibre-bearing, Yi Rong, direct-edible or be used as batching and stew edible or make tea and drink, and this freeze-dried powder quality is loose, expanded, be convenient to eat, be easily absorbed by the body.In addition, according to embodiments of the invention, dendrobium candidum freeze-dried powder of the present invention remains the reset condition of dendrobium candidum, light green color and luster, fresh taste and pharmacological effect, can normal temperature storage and transport, effectively extends storage life.
Additional aspect of the present invention and advantage will part provide in the following description, and part will become obvious from the following description, or be recognized by practice of the present invention.
Detailed description of the invention
Embodiments of the invention are described below in detail.Embodiment described below is exemplary, only for explaining the present invention, and can not be interpreted as limitation of the present invention.Unreceipted concrete technology or condition in embodiment, according to the technology described by the document in this area or condition or carry out according to product description.Agents useful for same or the unreceipted production firm person of instrument, being can by the conventional products of commercial acquisition.
Embodiment 1
Dendrobium candidum freeze-dried powder is prepared according to following steps:
(1) fresh dendrobium candidum of gathering is cleaned, drained, be cut into the section that 2-4cm is long, for subsequent use;
(2) dendrobium candidum drained is placed in nine sun (Joyoung) JYL-C010 cooking machine, adds pure water squeeze the juice with the ratio of solid-liquid ratio (1g:5ml), that has squeezed juice refrigeratedly spends the night, for subsequent use;
(3) FD-1A-50 type vacuum freeze drier (Shanghai produces than bright Instrument Ltd.) is carried out-56 DEG C of precooling half an hour;
(4) the juice refrigerated product of dendrobium candidum processed through step (2) are placed in vacuum freeze, cryostat is carried out at-56 DEG C, be evacuated to vacuum 7-9Pa, moisture in juice refrigerated for dendrobium candidum product is carried out lyophilization, time is 18-30 hour, to obtain dendrobium candidum freeze-dried powder;
(5) the food vacuum compressed bag of clean hygiene will be loaded through the dendrobium candidum freeze-dried powder product of step (4) lyophilization process, then seal.
Embodiment 2
The dendrobium candidum freeze-dried powder that embodiment 1 is prepared, the air-dried Dendrobium obtained with traditional stem of noble dendrobium drying means carries out sign and compares, specific as follows:
1, traditional stem of noble dendrobium drying means is utilized to prepare air-dried Dendrobium
(1) the preparation of freeze-drying section:
The method provided in referenced patent document " a kind of vacuum freeze-drying processing method of dendrobium officinale " (number of patent application is 201110114094.1) prepares dendrobium candidum freeze-drying section: 1. iron-sheet dendrobe fresh product stem section (2-4cm is long) is cleaned, drained, segment; 2. the dendrobium candidum stem section drained is placed in freeze drying equipment, at-30 DEG C--35 DEG C are carried out pre-freeze and cryostat: the dendrobium candidum lyophilization 3. 2. will processed through step; 4. the dendrobium candidum through step 3. lyophilization process is carried out residual moisture heating evaporation.
(2) the preparation of air-dry section:
Get the fresh stem section of dendrobium candidum (2-4cm is long) to load in ventilation mesh bag, hang on outdoor, natural air drying obtains air-dry section of dendrobium candidum after 1 month;
(3) the preparation of drying section:
Getting the fresh stem section of dendrobium candidum (2-4cm is long) is placed in stainless steel pallet, and in 60 DEG C of baking ovens, constant temperature drying obtained dendrobium candidum drying section after 30 hours.
2, the comparison of polyoses content
According to following steps, measure the obtained dendrobium candidum freeze-dried powder of embodiment 1 respectively, and the obtained freeze-drying section of above-mentioned steps 1, air-dry section and drying section polyoses content:
(1) precision takes testing sample (the freeze-drying section that the dendrobium candidum freeze-dried powder that embodiment 1 obtains, above-mentioned steps 1 obtain, air-dry section or drying section) 1g, add water 200ml, add hot reflux 2h, put to room temperature, be all transferred in 250ml volumetric flask, with a small amount of moisture washing container several times, washing lotion is transferred in same volumetric flask, be diluted with water to scale, shake up, filter; Accurate absorption filtrate 2ml, puts in 15ml centrifuge tube, adds absolute ethyl alcohol 8ml, shake up, in-20 DEG C of refrigerators, place 1h, take out, centrifugal (4000r/min) 20min, abandoning supernatant, precipitation heating water dissolves, and is transferred in 5ml volumetric flask, puts to room temperature, add water to scale, shake up and get final product.Thus, the need testing solution of dendrobium candidum freeze-dried powder, freeze-drying section, air-dry section and drying section is prepared respectively.
(2) the preparation of reference substance solution
Precision takes D-Glucose reference substance 14.01mg, puts in 100ml volumetric flask, adds appropriate distilled water and dissolves, be diluted to scale, shake up and get final product.
(3) Phenol sulfuric acid procedure
The preparation of nitrite ion: slowly added by 50ml sulfuric acid in 10ml distilled water, stirs and is cooled to room temperature, then add 0.6g and heavily steam phenol, stirs and makes it dissolve, be namely made into nitrite ion.
Accurate absorption need testing solution 0.5ml, is placed in tool plug test tube respectively, adds water and complement to 2ml, add nitrite ion 5.0ml, shake up, put in boiling water bath and heat 15min, take out, put in ice-water bath and cool 5min, take out, put to room temperature, separately get the same operation repetitive of 2.0ml distilled water, as blank, measure absorbance at 490nm place.
(4) linear relationship
Precision take reference substance solution 0.2,0.4,0.6,0.8,1.0ml, be placed in tool plug test tube respectively, adding distil water supplies 2ml, add developer 5ml, shake up, put in boiling water bath and heat 15min, take out, put in frozen water and cool 5min, take out, put to room temperature, separately get the same operation repetitive of 2.0ml distilled water, as blank, 490nm place measures absorbance.Take absorbance as ordinate, concentration is abscissa, show that regression equation is y=4.8964x+0.0465, r2=0.9927.
Then, based on the absorbance of each need testing solution that step (3) records, according to regression equation, calculate the polysaccharide concentration that extracts in each testing sample as following table:
| Sample | The polysaccharide concentration (mg/ml) of each testing sample |
| Freeze-dried powder | 0.335 |
| Freeze-drying section | 0.028 |
| Air-dry section | Content very little, not in standard curve range, cannot detect |
| Drying section | 0.014 |
As shown above, each testing sample of equal quality, the polyoses content extracted in the freeze-dried powder that embodiment 1 is obtained is the highest, and the air-dried Dendrobium obtained far above three kinds of traditional stem of noble dendrobium drying means, thus, show that the utilization rate of dendrobium polysaccharide in dendrobium candidum freeze-dried powder of the present invention is the highest.
3, the comparison of other characteristic indexs
The dendrobium candidum freeze-dried powder that this experiment is obtained to embodiment 1, and the obtained freeze-drying section of above-mentioned steps 1, air-dry section and drying section sign, such as color and luster, mouthfeel, solubility and shelf-life compare, specific as follows:
1. first carry out entrance to each processing sample to chew, draw the comparing result of mouthfeel, see the following form;
2. freeze-drying section obtained for above-mentioned steps 1, air-dry section and drying section are pulverized respectively in medicinal herb grinder, and sieve (80 order), so that then the dry powder obtaining three kinds of testing samples compare to the color and luster of the dendrobium candidum freeze-dried powder that dry powder and the embodiment 1 of these three kinds of testing samples obtain, the results are shown in following table;
3. get each processing sample dry powder (dry powder of above-mentioned obtained freeze-drying section, air-dry section and drying section, and the obtained dendrobium candidum freeze-dried powder of embodiment 1) 10g respectively, be dissolved in 50ml distilled water, contrast its solubility, the results are shown in following table;
4. by each processing sample (dendrobium candidum freeze-dried powder that embodiment 1 is obtained, and the obtained freeze-drying section of above-mentioned steps 1, air-dry section and drying section) in air drying environment, store 1 year after, again compare color and luster, mouthfeel according to the method described above, and the polyoses content of each sample is again measured according to the method in step 2, thus its shelf-life is compared, the results are shown in following table:
It should be noted that, traditional stem of noble dendrobium drying be mostly to adopt air-dry, dry, dry, the mode such as oven dry carries out, but it is long also to there is such as drying time in the air-dried Dendrobium that these methods obtain, sanitation degree is poor, nutrient component damages degree is high, and local flavor, taste easily change, and color and luster is variable, profile is shunk, the shortcomings such as metamorphosis is large.And from above-mentioned comparative result, stem of noble dendrobium freeze-dried powder obtained by the present invention effectively can keep the biologically active of dendrobium polysaccharide, color keeps light green, and not fibre-bearing, quality is loosened, and can be dissolved completely in water, relative to the air-dried Dendrobium that above-mentioned conventional method is obtained, dendrobium polysaccharide utilization rate is high, and storage life have also been obtained prolongation.Thus relative to conventional method, the method preparing dendrobium candidum freeze-dried powder of the present invention, has significant superiority.
Claims (7)
1. prepare a method for dendrobium candidum freeze-dried powder, it is characterized in that, comprising:
(1) fresh dendrobium candidum of gathering is cleaned, drained, be cut into the section that 2-4cm is long, for subsequent use;
(2) the dendrobium candidum section drained is placed in juice extractor, adds pure water with the ratio of solid-liquid ratio 1g:5ml and squeeze the juice;
(3) the dendrobium candidum juice obtained in step (2) is carried out-20 DEG C of freeze overnight, for subsequent use;
(4) vacuum freeze is carried out-56 DEG C of precooling half an hour; And
(5) the juice refrigerated product of dendrobium candidum obtained in step (3) are placed in the vacuum freeze through precooling treatment, in-56 DEG C, carry out vacuum freeze drying process 18-30 hour, to obtain dendrobium candidum freeze-dried powder under the condition of vacuum 7-9Pa.
2. method according to claim 1, is characterized in that, described juice extractor is nine positive JYL-C010 cooking machines.
3. method according to claim 1, is characterized in that, at-20 DEG C, the dendrobium candidum juice obtained in step (2) is carried out freeze overnight.
4. method according to claim 1, is characterized in that, described vacuum freeze is FD-1A-50 type vacuum freeze drier, and preferred Shanghai produces than bright Instrument Ltd..
5. method according to claim 1, is characterized in that, carries out described vacuum freeze drying process 24 hours.
6. method according to claim 1, is characterized in that, comprises further:
(6) the dendrobium candidum freeze-dried powder that step (5) obtains is carried out vacuum packaging process.
7. a dendrobium candidum freeze-dried powder, it is prepared by the method described in any one of claim 1-6.
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| CN104758257A (en) * | 2015-04-02 | 2015-07-08 | 贵州同威生物科技有限公司 | Fresh dendrobium officinale freeze-dried powder and preparation method thereof |
| CN105267692A (en) * | 2015-11-09 | 2016-01-27 | 安徽千方生物科技有限公司 | Dendrobium candidum preparation for enhancing cell repairing and preparing method of dendrobium candidum preparation |
| CN105602859A (en) * | 2016-01-26 | 2016-05-25 | 北京本草通汇科技中心 | Rhizopus microsporus C97102734 and application |
| CN105983061A (en) * | 2015-03-04 | 2016-10-05 | 安徽千方生物科技有限公司 | Preparation process for active dendrobium officinale freeze-dried powder |
| CN106421414A (en) * | 2016-10-19 | 2017-02-22 | 南京希元生物医药科技有限公司 | Process for preparing dendrobium officinale freeze-dried powder |
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| CN104758257B (en) * | 2015-04-02 | 2017-08-22 | 贵州同威生物科技有限公司 | Fresh dendrobium candidum freeze-dried powder and preparation method |
| CN104758257A (en) * | 2015-04-02 | 2015-07-08 | 贵州同威生物科技有限公司 | Fresh dendrobium officinale freeze-dried powder and preparation method thereof |
| CN105267692A (en) * | 2015-11-09 | 2016-01-27 | 安徽千方生物科技有限公司 | Dendrobium candidum preparation for enhancing cell repairing and preparing method of dendrobium candidum preparation |
| CN105602859B (en) * | 2016-01-26 | 2019-05-28 | 北京本草通汇科技中心 | Rhizopus microsporus C97102734 and application |
| CN105602859A (en) * | 2016-01-26 | 2016-05-25 | 北京本草通汇科技中心 | Rhizopus microsporus C97102734 and application |
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| CN110064026A (en) * | 2019-03-19 | 2019-07-30 | 安徽拂旦生物科技有限公司 | A kind of Efficient utilization method of meter of dry measure used in former times |
| CN110236044A (en) * | 2019-06-17 | 2019-09-17 | 广西山记农业发展有限公司 | A kind of fresh juice drink preparation method of dendrobium nobile and the fresh juice drink of dendrobium nobile |
| CN112137100A (en) * | 2019-06-27 | 2020-12-29 | 攀枝花市同丰斛农业开发有限公司 | Preparation and restoration method of fresh dendrobe juice freeze-dried cake |
| CN114306513A (en) * | 2021-11-30 | 2022-04-12 | 安徽省双辉生物科技有限公司 | Production process of low-oxidation dried dendrobium |
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