CN104160873A - Cultivation and management method for cordyceps militaris - Google Patents
Cultivation and management method for cordyceps militaris Download PDFInfo
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- CN104160873A CN104160873A CN201410385204.1A CN201410385204A CN104160873A CN 104160873 A CN104160873 A CN 104160873A CN 201410385204 A CN201410385204 A CN 201410385204A CN 104160873 A CN104160873 A CN 104160873A
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Abstract
The invention discloses a cultivation and management method for cordyceps militaris. The method comprises the steps of culture medium preparation, sub-packaging, sealing, sterilization, inoculation and shading cultivation; after inoculation, cultivation is performed for 5 d-6 d, and then visible light coloring is performed for 2 d-4 d; after coloring is completed, a cultivation room is sterilized, and then cultivation bottles or cultivation bags are ventilated, wherein the temperature is 18 DEG C-22 DEG C, and the relative air humidity is 80 percent-90 percent; sporocarps are formed on the surfaces of culture media at 13 d-25 d after the inoculation, wherein the controlled temperature is 20 DEG C, the humidity is 65 percent-75 percent, luminosity is 200 lux-300 lux, and illumination time is 10 h-12 h; when the sporocarps grow to 2 cm at 20 d-40 d after the inoculation, the temperature is raised to 20 DEG C-23 DEG C, the humidity is 70 percent-80 percent, the luminosity is 200 lux-300 lux, and the illumination time is 10 h-12 h per day; the illumination time is 15 h or more per day at the late growth stage of the sporocarps, the intumescent parts on the tops of the sporocarps become coarse, and the cordyceps militaris enters the mature stage and is harvested in time. The method is simple and easy to implement, low in pollution rate and suitable for industrial production.
Description
Technical field
The present invention relates to Cordyceps Militaris technical field, be specifically related to a kind of cordyceps militaris plantation management method.
Background technology
Cordyceps militaris is Ascomycotina, ergot Zoopagales, the type sepecies of Clavicipitaceae, Cordyceps.Formal name used at school is Cordycepsmilitaris, has another name called northern Chinese caterpillar Fungus, northern Chinese caterpillar fungus etc.Worldwide distribution natural resources quantity is little.By stroma (being careless part) and sclerotium (being the corpse part of worm) complex dimerous.Cordyceps militaris has following effect: righting benefit gas, very high immunity and hematopoiesis function; Tonifying lung is relievingd asthma, and improves respiratory system; Improve and regulate heart and brain system; Blood fat in control agent; Have and invigorate the lung and the kidney, improve effect of immunity, memory; Spleen-benefiting mind-tranquilizing, has good therapeutic action for insomnia and dreamful sleep, neurasthenia.
Summary of the invention
The object of this invention is to provide a kind of cordyceps militaris plantation management method.
The present invention is by the following technical solutions:
A kind of cordyceps militaris plantation management method, comprises the steps:
Step 1, medium preparation: select that fresh nothing is gone mouldy, free of contamination rice, by rice and dregs of beans 9:1 mixing in mass ratio, or rice, wheat and dregs of beans in mass ratio 4.5:4.5:1 mix, or rice, corn flour, dregs of beans in mass ratio 6.3:2.7:1 mix, and add water by 1.3～1.8 times of mixture quality; Add afterwards 0.15wt% potassium dihydrogen phosphate, 0.05wt% magnesium sulfate, 20mgVB1/100kg water, 5mgVB6/100kg water, 5mgVB12/100kg water, mix, regulate pH to 6.8～7.5;
Step 2, pack by every bottle of 30～40g the medium preparing into 500-650ml rice base bottle, or every bag of 100g of plastic sack, rice base bottle 13cm after point installing
3transparent polypropylene film add the sealing of elastic tape or bicycle tube bar, on plastic bag cover without cotton-padded covering ring seal mouth;
Step 3, by installing rice base bottle or plastic sack normal-pressure sterilization or the high pressure steam sterilization of medium, be cooled to afterwards 15～20 DEG C;
Step 4, aseptic inoculation: the liquid Cordyceps militaris spawn of 500-650ml rice base bottle graft kind 4～8ml, plastic sack inoculation 10～15ml;
Step 5, the shading cultivation at 15～18 DEG C by the rice base bottle after inoculation or plastic sack; Mycelial growth is to medium 1/2～2/3 o'clock, temperature is adjusted to 20～22 DEG C and is cultured to mycelia and covers with medium, wherein, cultivates 5～6d and transfer visible ray annesl to, annesl 2～4d after inoculation;
Step 6, turned after look and will sterilize to culturing room, to blake bottle or culture bag ventilation, temperature is at 18～22 DEG C afterwards, and relative air humidity is 80～90%; 13～25d after inoculation, fruit body forms in media surface, controls temperature at 20 DEG C, humidity 65～75%, luminosity 200～300lux, light application time 10～12h; 20～40d after inoculation, fruit body grows to after 2cm, and temperature is brought up to 20～23 DEG C, humidity 70～80%, luminosity 200～300lux, illumination every day 10～12h; More than sporophore growth later stage illumination every day 15h, fruit body top is expanded part and is seen coarsely, and Cordyceps militaris enters the stage of ripeness, gathers in time.
Described in step 1, rice is hybridization rice, brown rice or polished rice.
Described in step 4, Cordyceps militaris spawn is " No. 6, Kingsoft " or " No. 8, Kingsoft ".
Described in step 4, liquid Cordyceps militaris spawn preparation method is: 3～5 0.5cm of picking under aseptic condition
3female piece of planting is put into 500～1000ml liquid nutrient medium bottle, adopts shaking flask concussion to cultivate, and at 20～21 DEG C, cultivates 3～5d; When use, dilute 3～5 times, wherein, liquid nutrient medium preparation method is: 200g potato is put into 1000ml water, boil 25min, cross leaching cleaner liquid, add afterwards 10g glucose, 3g peptone, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, 0.5g agar, 20mgVB1, low baking temperature fusing, regulates pH to 6.8; Or 15g peptone, 10g glucose, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, 0.5 agar, 20mgVB1 are put into 1000ml water, mix, regulate pH to 6.8.
Beneficial effect of the present invention: the inventive method is simple, pollution rate is low, is applicable to suitability for industrialized production.
Embodiment
Below in conjunction with embodiment, the present invention is done further and explained.Following embodiment does not limit the present invention in any form, and all employings are equal to replaces or technical scheme that the mode of equivalent transformation obtains, all among protection scope of the present invention.
A kind of cordyceps militaris plantation management method, comprises the steps:
Step 1, medium preparation: select that fresh nothing is gone mouldy, free of contamination rice, rice can be hybridization rice, brown rice or polished rice, by rice and dregs of beans 9:1 mixing in mass ratio, or rice, wheat and dregs of beans in mass ratio 4.5:4.5:1 mix, or rice, corn flour, dregs of beans in mass ratio 6.3:2.7:1 mix, and add water by 1.3～1.8 times of mixture quality; Add afterwards 0.15wt% potassium dihydrogen phosphate, 0.05wt% magnesium sulfate, 20mgVB1/100kg water, 5mgVB6/100kg water, 5mgVB12/100kg water, mix, regulate pH to 6.8～7.5;
Step 2, pack by every bottle of 30～40g the medium preparing into 500-650ml rice base bottle, or every bag of 100g of plastic sack, rice base bottle 13cm after point installing
3transparent polypropylene film add the sealing of elastic tape or bicycle tube bar, on plastic bag cover without cotton-padded covering ring seal mouth;
Step 3, by installing rice base bottle or plastic sack normal-pressure sterilization or the high pressure steam sterilization of medium, be cooled to afterwards 15～20 DEG C;
Step 4, aseptic inoculation: the liquid " No. 6, Kingsoft " of 500-650ml rice base bottle graft kind 4～8ml or " No. 8, Kingsoft " Cordyceps militaris spawn, plastic sack inoculation 10～15ml; Described liquid Cordyceps militaris spawn preparation method is: 3～5 0.5cm of picking under aseptic condition
3female piece of planting is put into 500～1000ml liquid nutrient medium bottle, adopts shaking flask concussion to cultivate, and at 20～21 DEG C, cultivates 3～5d; When use, dilute 3～5 times, wherein, liquid nutrient medium preparation method is: 200g potato is put into 1000ml water, boil 25min, cross leaching cleaner liquid, add afterwards 10g glucose, 3g peptone, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, 0.5g agar, 20mgVB1, low baking temperature fusing, regulates pH to 6.8; Or 15g peptone, 10g glucose, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, 0.5 agar, 20mgVB1 are put into 1000ml water, mix, regulate pH to 6.8;
Step 5, the shading cultivation at 15～18 DEG C by the rice base bottle after inoculation or plastic sack; Mycelial growth is to medium 1/2～2/3 o'clock, temperature is adjusted to 20～22 DEG C and is cultured to mycelia and covers with medium, wherein, cultivates 5～6d and transfer visible ray annesl to, annesl 2～4d after inoculation;
Step 6, turned after look and will sterilize to culturing room, to blake bottle or culture bag ventilation, temperature is at 18～22 DEG C afterwards, and relative air humidity is 80～90%; 13～25d after inoculation, fruit body forms in media surface, controls temperature at 20 DEG C, humidity 65～75%, luminosity 200～300lux, light application time 10～12h; 20～40d after inoculation, fruit body grows to after 2cm, and temperature is brought up to 20～23 DEG C, humidity 70～80%, luminosity 200～300lux, illumination every day 10～12h; More than sporophore growth later stage illumination every day 15h, fruit body top is expanded part and is seen coarsely, and Cordyceps militaris enters the stage of ripeness, gathers in time.
Embodiment 1
1, medium preparation: select that fresh nothing is gone mouldy, free of contamination hybridization rice, will hybridize meter and dregs of beans 9:1 mixing in mass ratio, add water by 1.3 times of mixture quality; Add afterwards 0.15wt% potassium dihydrogen phosphate, 0.05wt% magnesium sulfate, 20mgVB1/100kg water, 5mgVB6/100kg water, 5mgVB12/100kg water, mix, regulate pH to 6.8;
2, pack by every bottle of 30g the medium preparing into 500ml rice base bottle, divide and install rice base bottle 13cm afterwards
3transparent polypropylene film add elastic tape sealing;
3, by installing the rice base bottle normal-pressure sterilization of medium, be cooled to afterwards 15 DEG C;
4, aseptic inoculation: liquid " No. 6, Kingsoft " Cordyceps militaris spawn of rice base bottle graft kind 4ml;
5, the rice base bottle shading at 15 DEG C after inoculation is cultivated; Mycelial growth is during to medium 1/2, temperature is adjusted to 20 DEG C and is cultured to mycelia and covers with medium, wherein, cultivates 5d and transfer visible ray annesl to, annesl 2d after inoculation;
6, turned look and will sterilize to culturing room afterwards, to blake bottle or culture bag ventilation, temperature is at 18 DEG C afterwards, and relative air humidity is 80%; 13d after inoculation, fruit body forms in media surface, controls temperature at 20 DEG C, humidity 65%, luminosity 200lux, light application time 10h; 20d after inoculation, fruit body grows to after 2cm, and temperature is brought up to 20 DEG C, humidity 70%, luminosity 200lux, illumination every day 10h; More than sporophore growth later stage illumination every day 15h, fruit body top is expanded part and is seen coarsely, and Cordyceps militaris enters the stage of ripeness, gathers in time.
Embodiment 2
1, medium preparation: select that fresh nothing is gone mouldy, pollution-free brown rice, by brown rice, wheat and dregs of beans in mass ratio 4.5:4.5:1 mix, add water by 1.8 times of mixture quality; Add afterwards 0.15wt% potassium dihydrogen phosphate, 0.05wt% magnesium sulfate, 20mgVB1/100kg water, 5mgVB6/100kg water, 5mgVB12/100kg water, mix, regulate pH to 7.5;
2, pack by every bottle of 40g the medium preparing into 650ml rice base bottle, divide and install rice base bottle 13cm afterwards
3bicycle tube bar sealing;
3, by installing the rice base bottle high pressure steam sterilization of medium, be cooled to afterwards 20 DEG C;
4, aseptic inoculation: liquid " No. 8, Kingsoft " Cordyceps militaris spawn of rice base bottle graft kind 8ml;
5, by the rice base bottle after inoculation or plastic sack, shading at 18 DEG C is cultivated; Mycelial growth is during to medium 2/3, temperature is adjusted to 22 DEG C and is cultured to mycelia and covers with medium, wherein, cultivates 6d and transfer visible ray annesl to, annesl 4d after inoculation;
6, turned look and will sterilize to culturing room afterwards, to blake bottle or culture bag ventilation, temperature is at 22 DEG C afterwards, and relative air humidity is 90%; 25d after inoculation, fruit body forms in media surface, controls temperature at 20 DEG C, humidity 75%, light 300lux, light application time 12h; 40d after inoculation, fruit body grows to after 2cm, and temperature is brought up to 23 DEG C, humidity 80%, luminosity 300lux, illumination every day 12h; More than sporophore growth later stage illumination every day 15h, fruit body top is expanded part and is seen coarsely, and Cordyceps militaris enters the stage of ripeness, gathers in time.
Embodiment 3
1, medium preparation: select that fresh nothing is gone mouldy, free of contamination polished rice, by polished rice, corn flour, dregs of beans in mass ratio 6.3:2.7:1 mix, add water by 1.5 times of mixture quality; Add afterwards 0.15wt% potassium dihydrogen phosphate, 0.05wt% magnesium sulfate, 20mgVB1/100kg water, 5mgVB6/100kg water, 5mgVB12/100kg water, mix, regulate pH to 7.0;
2, pack by every bag of 100g the medium preparing into plastic sack, after point installing on plastic bag cover without cotton-padded covering ring seal mouth;
3, will install the plastic sack high pressure steam sterilization of medium, be cooled to afterwards 15 DEG C;
4, aseptic inoculation: plastic sack inoculation 10～15ml liquid " No. 6, Kingsoft " Cordyceps militaris spawn;
5, by the rice base bottle after inoculation or plastic sack, shading at 16 DEG C is cultivated; Mycelial growth is during to medium 2/3, temperature is adjusted to 21 DEG C and is cultured to mycelia and covers with medium, wherein, cultivates 6d and transfer visible ray annesl to, annesl 4d after inoculation;
6, turned look and will sterilize to culturing room afterwards, to blake bottle or culture bag ventilation, temperature is at 20 DEG C afterwards, and relative air humidity is 85%; 20d after inoculation, fruit body forms in media surface, controls temperature at 20 DEG C, humidity 70%, luminosity 250lux, light application time 11h; 30d after inoculation, fruit body grows to after 2cm, and temperature is brought up to 21 DEG C, humidity 75%, luminosity 250lux, illumination every day 11h; More than sporophore growth later stage illumination every day 15h, fruit body top is expanded part and is seen coarsely, and Cordyceps militaris enters the stage of ripeness, gathers in time.
Claims (4)
1. a cordyceps militaris plantation management method, is characterized in that, comprises the steps:
Step 1, medium preparation: select that fresh nothing is gone mouldy, free of contamination rice, by rice and dregs of beans 9:1 mixing in mass ratio, or rice, wheat and dregs of beans in mass ratio 4.5:4.5:1 mix, or rice, corn flour, dregs of beans in mass ratio 6.3:2.7:1 mix, and add water by 1.3～1.8 times of mixture quality; Add afterwards 0.15wt% potassium dihydrogen phosphate, 0.05wt% magnesium sulfate, 20mgVB1/100kg water, 5mgVB6/100kg water, 5mgVB12/100kg water, mix, regulate pH to 6.8～7.5;
Step 2, pack by every bottle of 30～40g the medium preparing into 500-650ml rice base bottle, or every bag of 100g of plastic sack, rice base bottle 13cm after point installing
3transparent polypropylene film add the sealing of elastic tape or bicycle tube bar, on plastic bag cover without cotton-padded covering ring seal mouth;
Step 3, by installing rice base bottle or plastic sack normal-pressure sterilization or the high pressure steam sterilization of medium, be cooled to afterwards 15～20 DEG C;
Step 4, aseptic inoculation: the liquid Cordyceps militaris spawn of 500-650ml rice base bottle graft kind 4～8ml, plastic sack inoculation 10～15ml;
Step 5, the shading cultivation at 15～18 DEG C by the rice base bottle after inoculation or plastic sack; Mycelial growth is to medium 1/2～2/3 o'clock, temperature is adjusted to 20～22 DEG C and is cultured to mycelia and covers with medium, wherein, cultivates 5～6d and transfer visible ray annesl to, annesl 2～4d after inoculation;
Step 6, turned after look and will sterilize to culturing room, to blake bottle or culture bag ventilation, temperature is at 18～22 DEG C afterwards, and relative air humidity is 80～90%; 13～25d after inoculation, fruit body forms in media surface, controls temperature at 20 DEG C, humidity 65～75%, luminosity 200～300lux, light application time 10～12h; 20～40d after inoculation, fruit body grows to after 2cm, and temperature is brought up to 20～23 DEG C, humidity 70～80%, luminosity 200～300lux, illumination every day 10～12h; More than sporophore growth later stage illumination every day 15h, fruit body top is expanded part and is seen coarsely, and Cordyceps militaris enters the stage of ripeness, gathers in time.
2. cordyceps militaris plantation management method according to claim 1, is characterized in that, rice is hybridization rice, brown rice or polished rice described in step 1.
3. cordyceps militaris plantation management method according to claim 1, is characterized in that, Cordyceps militaris spawn is " No. 6, Kingsoft " or " No. 8, Kingsoft " described in step 4.
4. cordyceps militaris plantation management method according to claim 1, is characterized in that, liquid Cordyceps militaris spawn preparation method is described in step 4: 3～5 0.5cm of picking under aseptic condition
3female piece of planting is put into 500～1000ml liquid nutrient medium bottle, adopts shaking flask concussion to cultivate, and at 20～21 DEG C, cultivates 3～5d; When use, dilute 3～5 times, wherein, liquid nutrient medium preparation method is: 200g potato is put into 1000ml water, boil 25min, cross leaching cleaner liquid, add afterwards 10g glucose, 3g peptone, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, 0.5g agar, 20mgVB1, low baking temperature fusing, regulates pH to 6.8; Or 15g peptone, 10g glucose, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, 0.5 agar, 20mgVB1 are put into 1000ml water, mix, regulate pH to 6.8.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604522A (en) * | 2015-01-19 | 2015-05-13 | 河南省民兴茧丝绸有限责任公司 | Method for producing cordyceps militaris |
| JP5800211B1 (en) * | 2015-02-13 | 2015-10-28 | 天然物産業つくば株式会社 | Artificial culture method of fruit body |
| CN105660188A (en) * | 2016-02-23 | 2016-06-15 | 张家港顺泰元生物科技有限公司 | Cultivation method for cordyceps militaris |
| CN105724054A (en) * | 2016-02-23 | 2016-07-06 | 张家港顺泰元生物科技有限公司 | Liquid culture solution for dried cordyceps militaris and culture method for dried cordyceps militaris |
| CN105838624A (en) * | 2016-04-26 | 2016-08-10 | 贵州省烟草公司贵阳市公司 | Cordycepsmilitaris(L.)Link fruiting body culture method capable of preventing mixed fungus pollution |
| CN107548885A (en) * | 2017-08-30 | 2018-01-09 | 宁波佗鹊堂生物科技有限公司 | Cordyceps militaris lighting culture |
| CN108207490A (en) * | 2018-01-02 | 2018-06-29 | 河南天蚕生物科技有限公司 | Univoltine Cordyceps militaris breeding method |
| CN110447472A (en) * | 2019-08-12 | 2019-11-15 | 江门市山海堂虫草有限公司 | A kind of cultivating container and its application method of Cordyceps militaris |
| CN112825730A (en) * | 2019-11-25 | 2021-05-25 | 湖南金芙农业科技有限公司 | Method for cultivating cordyceps militaris with high adenosine content |
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2014
- 2014-08-06 CN CN201410385204.1A patent/CN104160873A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604522A (en) * | 2015-01-19 | 2015-05-13 | 河南省民兴茧丝绸有限责任公司 | Method for producing cordyceps militaris |
| JP5800211B1 (en) * | 2015-02-13 | 2015-10-28 | 天然物産業つくば株式会社 | Artificial culture method of fruit body |
| CN105660188A (en) * | 2016-02-23 | 2016-06-15 | 张家港顺泰元生物科技有限公司 | Cultivation method for cordyceps militaris |
| CN105724054A (en) * | 2016-02-23 | 2016-07-06 | 张家港顺泰元生物科技有限公司 | Liquid culture solution for dried cordyceps militaris and culture method for dried cordyceps militaris |
| CN105838624A (en) * | 2016-04-26 | 2016-08-10 | 贵州省烟草公司贵阳市公司 | Cordycepsmilitaris(L.)Link fruiting body culture method capable of preventing mixed fungus pollution |
| CN107548885A (en) * | 2017-08-30 | 2018-01-09 | 宁波佗鹊堂生物科技有限公司 | Cordyceps militaris lighting culture |
| CN108207490A (en) * | 2018-01-02 | 2018-06-29 | 河南天蚕生物科技有限公司 | Univoltine Cordyceps militaris breeding method |
| CN110447472A (en) * | 2019-08-12 | 2019-11-15 | 江门市山海堂虫草有限公司 | A kind of cultivating container and its application method of Cordyceps militaris |
| CN112825730A (en) * | 2019-11-25 | 2021-05-25 | 湖南金芙农业科技有限公司 | Method for cultivating cordyceps militaris with high adenosine content |
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Application publication date: 20141126 |