CN102090266A - High-efficient cultivation method of schizophyllum commune - Google Patents
High-efficient cultivation method of schizophyllum commune Download PDFInfo
- Publication number
- CN102090266A CN102090266A CN 201010578619 CN201010578619A CN102090266A CN 102090266 A CN102090266 A CN 102090266A CN 201010578619 CN201010578619 CN 201010578619 CN 201010578619 A CN201010578619 A CN 201010578619A CN 102090266 A CN102090266 A CN 102090266A
- Authority
- CN
- China
- Prior art keywords
- bacterium
- days
- medium
- inoculation
- white ginseng
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a high-efficient cultivation method of schizophyllum commune. The method comprises the following steps of: inoculating a cultivar cultivated by domesticating wild schizophyllum commune into a cultivar culture medium which is prepared by components such as barley seeds or buckwheat seeds, broad leaf saw dust and the like and is subjected to high temperature and high pressure treatment; directly sheltering, shelving and arranging; exhausting air through a pricked hole; unsealing; cutting, and the like. Mushroom fruiting is managed at a proper temperature and high humidity; the grown schizophyllum commune has high quality; and the method is high in economic benefit, is easy to operate and practicable, and is an ideal schizophyllum commune manual planting technology.
Description
Technical field
The invention belongs to artificial cultivation edible mushroom technology, the efficient cultivation of especially a kind of white ginseng bacterium.
Background technology
Join Pseudomonas in vain in Basidiomycotina, Hymenomycetes, Agaricales, schizophyllum commune section, Pleurotus has another name called schizophyllum commune, and popular name eight load bavins are a kind of wild edible fungus distributed more widely.Its adaptation temperature spoke broad, mycelia can grow in 6-30 ℃ of scope, and low energy is 8 ℃ of growth fruit bodys, the fruit body optimum growth temp is 14-28 ℃, and 30 ℃ of limit high temperature are had relatively high expectations to moisture content, fruit body optimum growh air humidity is 80-90%, is lower than dehydration death in 55% short time.Because white ginseng bacterium is rich in multiple organic matter, contain 17 kinds in needed by human body 18 seed amino acids, contain 7 kinds of 8 kinds of trace elements of needed by human body, and contain antitumor, antibiotic synergist, many of schizophyllum communes, the delicious delicate fragrance of edible taste, often ediblely clear liver and improve vision, stomach-invigorating intestine-moisturizing, the function of nourishing and fit keeping function, infantile night sweat, hemorrhoid disease, constipation, neurasthenia, dizzy tinnitus, dyssomnia all there is remarkable result, can strengthen body immunity, can assist tumor treatment, be a kind of of great value food medicine dual purpose product.But the pure wild little flavor of a white ginseng bacterium shape is old, output is limited, can not meet the need of market, people have also obtained some progress aspect the white ginseng of the cultivation bacterium taming and dociling to educate, tame white ginseng bacterium quality, taste, output all are better than wild bacterium, as the patent No. is that artificial cultivation raising technology that ZL200410021821.X joins bacterium in vain is wherein a kind of, and through the wild white ginseng bacterium of seed selection domestication, and successful breeding cultivation has been produced quality, output and is better than wild white living bacterium greatly.But should the cover technology still have deficiency: the medium batching component of cultivated species is too much, reach the 5-7 kind, and the nutriment that can provide is not enough, the white ginseng bacterium of planting out except that a first planting shape, output better, several batches of backs are obvious weak, are difficult to reach high yield, stable yields, and the economy price ratio is not high, and, seem too cumbersome in the operation.
Summary of the invention
Provide few, the comprehensive nutrition of a cover component, the cultivated species medium that content is high for remedying above-mentioned deficiency, the invention reside in, bigger to ensure a white ginseng bacterium shape of producing, outward appearance is more attractive in appearance, and stable and high yields can be adopted many batches, economic benefit height, and the simple white ginseng bacteria cultivation method of operating technology.
The present invention finishes like this: preferred fresh and alive wild white ginseng bacterial strain is inoculated in potato agar albumen test tube medium with separate tissue or spore separation method, cultivating purifies obtains female the kind, mother planted cultivate in the buckwheat grain medium be inoculated in vial purify original seed, again behind the culture medium for cultivating bag that original seed access preparation is handled well, directly move into the plastic tunnel of thermophilic high humidity, observe the management fruiting.
The technical characterictic of this method is that the cultivated species medium has three kinds of prescriptions, by the percentage by weight of preparing burden is:
1. wheat berry 50%, broad-leaved weedtree sawdust 49%, gesso 1%;
2. barley corn 60%, broad-leaved weedtree sawdust 30%, wheat bran 9%, gesso 1%;
3. the buckwheat grain 60%, broad-leaved weedtree sawdust 35%, wheat bran 4%, gesso 1%;
Batching is mixed all, adding water by material-water ratio 1: 1.3-1.5 stirs, making water content is 55%-60%, pinch not drip with hand and be advisable, divide the bag of packing into, tighten mouth and insert wet sterilization pot pressurization 0.147MPa, temperature control keeps sterilization in 90 minutes for 128 ℃, naturally cooling, pressure is reduced to the taking-up sack that boiled at 0 o'clock, inserts the original seed make with sterile working when temperature is reduced to below 25 ℃, with direct put on the shelf 14-28 ℃ of the grand style temperature control of an immigration booth in non-drying adhesive tape sealing label inoculation eye back, damping 80-90%, carry out management of producing mushroom, can adopt the first planting in 15-18 days and join bacterium in vain, manage and protect 10 days with method and can adopt regrowth hair, receive the 3-4 stubble continuously, eliminate the bacterium tube after 50 days.
The inoculation of above-mentioned cultivated species takes to keep flat culture bag with inoculation bastinade cave one row's four eyed, and every is sealed with non-drying adhesive tape after putting into 20-25 grain original seed buckwheat grain.
Above-mentioned cultivated species inserts back 3 days, cover with inoculation when mycelia and at the moment puncture the exhaust of sealing compound cloth with 0.3cm diameter iron nail, threw off in 8-9 days and seal adhesive plaster, and cut and scratch each 1 of sack 1.5cm osculum in mycelia haloing limit, both sides up and down, form 12 fruiting eyes of three rows at inoculation eye.
Above-mentioned mother's kind, pedigree seed culture medium adopted pressure 0.147MPa, temperature 128-130 ℃ of steam pressure retort sterilization 40-60 minute.
The white ginseng bacterium that cultivates by this law is piece big, the profile endoplasm is all excellent, and the more former method of output improves more than 1 times, inserts behind the original seed and can need not pile bavin and cultivate a whole set of production routine and easy to operation, economic benefit height directly into the canopy grand style of putting on the shelf.
Provide embodiments of the invention below:
Embodiment
To pick up from open-air fresh and alive white ginseng bacterium after simple the processing, sterile working in inoculating hood, white ginseng bacterial strain tissue is inserted the test tube potato agar protein culture medium inclined-plane for preparing, obtain female the kind through cultivating to purify, again mother is planted and insert in the vial buckwheat grain medium for preparing, it is standby that the cultivation purification obtains original seed, and the vial of 1 500ml is installed in 0.43 jin of dried buckwheat grain, and the original seed for preparing can be for the inoculation of 40-50 bag cultivating kind.Taking by weighing 60kg does not have barley corn that the worm that goes mouldy stays and puts into 0.2% limewash and soak that to pick up the back flushing with clean water in 40 hours clean, take by weighing 30kg broad-leaved weedtree sawdust and 9kg wheat bran again, the 1kg gesso, adding water 130kg after the material loading mixing is mixed thoroughly fully stirs evenly, pinch not drip with hand and be advisable, water content is 60%, divide in the polyethylene bacterium bag of putting into 15 * 55cm, every bag of 1kg puts into the special-purpose wet sterilization pot 0.147MPa that pressurizes, 128 ℃ of temperature controls after sack tightened, kept 90 minutes, naturally cooling, pressure is 0 o'clock taking-up pocket that boils, and just can obtain 100 cultivation bags when temperature is reduced to below 25 ℃.Mother's kind of above-mentioned preparation, pedigree seed culture medium also will same high pressure-temperature sterilization treatment just can use after 40-60 minute in the wet sterilization pot equally.In transfer room, cultivation bag is kept flat with loop-carrier four inoculation eyes of horizontal bundle one row along bag, put into 20-25 grain original seed buckwheat grain for every, seal with non-drying adhesive tape, can obtain 100 bag cultivating bacterial classifications, directly moving into the interior grand style of booth puts on the shelf, under 14-28 ℃ of temperature, inoculate back 3 days mycelia cover with whole inoculation eye and inwardly a layer depth go into, this moment, the nail with diameter 0.3cm punctured the venting of sealing compound cloth, covered with sack half after 5-6 days mycelia, and had the sudden and violent property mushroom of part to emerge from the adhesive plaster edge, can throw off adhesive plaster, simultaneously the inoculation eye up and down both sides mycelia haloing edge with pocket knife sack is scratched each 1 of the osculum of 1.5cm, make whole bacterium bag be 12 of horizontal three rows' fruiting mouth, most of fruiting mouths all grew small mushroom bud after 2-3 days, this moment is when continuous spray form water, make humidity keep 80-90%, can pluck first batch of white ginseng bacterium after 5 days, the same manner management can be adopted second batch again after 10 days, if no mycotic infection, can adopt the 3-4 stubble continuously, can adopt 0.2kg altogether for every bag, eliminate the bacterium tube that more renews after 50 days.
Claims (5)
1. the efficient cultivation of a white ginseng bacterium, be that preferred open country is joined bacterial strain separate tissue or spore separation inoculation on potato agar albumen test tube medium in vain, through thermophilic cultivate female kind, again mother is planted and insert the glass buckwheat grain medium culture make and get original seed, original seed is inserted medium that preparation handles well make and go into the canopy grand style of putting on the shelf behind the cultivated species, the management fruiting is characterized in that, the cultivated species medium has three kinds of prescriptions, by ingredients by weight percentage is:
1. wheat berry 50%, broad-leaved weedtree sawdust 49%, gesso 1%;
2. barley corn 60%, broad-leaved weedtree sawdust 30%, wheat bran 9%, gesso 1%;
3. the buckwheat grain 60%, broad-leaved weedtree sawdust 35%, wheat bran 4%, gesso 1%;
Batching is mixed all, adding water by material-water ratio 1: 1.3-1.5 stirs, making water content is 55%-60%, pinch not drip with hand and be advisable, divide the bag of packing into, tighten mouth and insert wet sterilization pot pressurization 0.147MPa, temperature control keeps sterilization in 90 minutes for 128 ℃, naturally cooling, pressure is reduced to the taking-up sack that boiled at 0 o'clock, inserts the original seed make with sterile working when temperature is reduced to below 25 ℃, with direct put on the shelf 14-28 ℃ of the grand style temperature control of an immigration booth in non-drying adhesive tape sealing label inoculation eye back, damping 80-90%, carry out management of producing mushroom, can adopt the first planting in 15-18 days and join bacterium in vain, manage and protect 10 days with method and can adopt regrowth hair, adopt the 3-4 stubble continuously, eliminate the bacterium tube after 50 days.
2. the efficient cultivation of white ginseng bacterium as claimed in claim 1 is characterized in that, the used barley corn of cultivated species medium, wheat berry, buckwheat grain all must soak 40 hours with 0.2% limewash, pick up with flushing with clean water only again with other material adapted.
3. the efficient cultivation of white ginseng bacterium as claimed in claim 1 is characterized in that, row's four eyed is adopted in the inoculation of cultivated species, inserts 20-25 grain original seed buckwheat grain for every.
4. the efficient cultivation of white ginseng bacterium as claimed in claim 1, it is characterized in that cultivated species inoculation 3 days punctures the exhaust of sealing compound cloth with 0.3cm diameter iron nail, threw off in 8-9 days and seal adhesive plaster, and cut and scratch each 1 of sack 1.5cm osculum in mycelia haloing limit, both sides up and down at inoculation eye, form 12 fruiting eyes of three rows.
5. the efficient cultivation of white ginseng bacterium as claimed in claim 1 is characterized in that, the medium of female kind, original seed adopts pressure 0.147MPa, temperature 128-130 ℃ of steam pressure retort sterilization 40-60 minute.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2010105786192A CN102090266B (en) | 2010-12-05 | 2010-12-05 | High-efficient cultivation method of schizophyllum commune |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2010105786192A CN102090266B (en) | 2010-12-05 | 2010-12-05 | High-efficient cultivation method of schizophyllum commune |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102090266A true CN102090266A (en) | 2011-06-15 |
CN102090266B CN102090266B (en) | 2012-10-31 |
Family
ID=44123668
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2010105786192A Expired - Fee Related CN102090266B (en) | 2010-12-05 | 2010-12-05 | High-efficient cultivation method of schizophyllum commune |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102090266B (en) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102503601A (en) * | 2011-11-17 | 2012-06-20 | 大连春天生物技术有限公司 | Preparation method for bamboo fungus liquid strain culture medium |
CN102523935A (en) * | 2012-02-23 | 2012-07-04 | 蔡家顺 | Schizphylhls commne Fr. cultivation method capable of increasing yield exponentially |
CN105613035A (en) * | 2015-12-22 | 2016-06-01 | 镇江盛弘景观植物有限公司 | Cultivation and domestication method for wild cantharellus cibarius |
CN105875199A (en) * | 2016-05-12 | 2016-08-24 | 庞开云 | High-and-stable-yield cultivation method for Schizophyllumcommuneh |
CN106754409A (en) * | 2016-12-08 | 2017-05-31 | 段必儒 | White ginseng bacterium efficiently educates plant method |
CN107828664A (en) * | 2017-10-25 | 2018-03-23 | 湖南省农业生物技术研究中心 | Schizophyllum commune XT 1 and its cultural method and application |
CN109168961A (en) * | 2018-10-31 | 2019-01-11 | 芒市喜丰农业发展有限公司 | A kind of cultural method of white ginseng |
CN109220550A (en) * | 2018-10-31 | 2019-01-18 | 芒市喜丰农业发展有限公司 | A kind of method that white ginseng culture material waste residue recycles |
CN109348989A (en) * | 2018-12-03 | 2019-02-19 | 玉溪农业职业技术学院 | The efficient stable yields cultural method of white ginseng bacterium |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN85103492A (en) * | 1985-05-22 | 1987-04-15 | 南京大学 | The culture process of Split-gill and utilization thereof |
CN1306084A (en) * | 2000-01-18 | 2001-08-01 | 罗星野 | Artificially cultured schizophyllum sporophores and its culture process |
CN1557122A (en) * | 2004-02-11 | 2004-12-29 | 腾冲县中和乡农民文化技术学校 | Schizophyllum commune artificial cultivation and breeding technology |
-
2010
- 2010-12-05 CN CN2010105786192A patent/CN102090266B/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN85103492A (en) * | 1985-05-22 | 1987-04-15 | 南京大学 | The culture process of Split-gill and utilization thereof |
CN1306084A (en) * | 2000-01-18 | 2001-08-01 | 罗星野 | Artificially cultured schizophyllum sporophores and its culture process |
CN1557122A (en) * | 2004-02-11 | 2004-12-29 | 腾冲县中和乡农民文化技术学校 | Schizophyllum commune artificial cultivation and breeding technology |
Non-Patent Citations (2)
Title |
---|
《农村实用技术》 20081231 裴凤等 白参菌多层次立体栽培技术 第33页 1-5 , 第8期 2 * |
《安徽农业科学》 20071231 李竹英等 裂褶菌的规模化栽培技术 第700,711页 1-5 第35卷, 第3期 2 * |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102503601A (en) * | 2011-11-17 | 2012-06-20 | 大连春天生物技术有限公司 | Preparation method for bamboo fungus liquid strain culture medium |
CN102523935A (en) * | 2012-02-23 | 2012-07-04 | 蔡家顺 | Schizphylhls commne Fr. cultivation method capable of increasing yield exponentially |
CN105613035A (en) * | 2015-12-22 | 2016-06-01 | 镇江盛弘景观植物有限公司 | Cultivation and domestication method for wild cantharellus cibarius |
CN105875199A (en) * | 2016-05-12 | 2016-08-24 | 庞开云 | High-and-stable-yield cultivation method for Schizophyllumcommuneh |
CN106754409A (en) * | 2016-12-08 | 2017-05-31 | 段必儒 | White ginseng bacterium efficiently educates plant method |
CN106754409B (en) * | 2016-12-08 | 2020-01-10 | 段必儒 | Method for cultivating and planting holothuria leucorrhoeae |
CN107828664A (en) * | 2017-10-25 | 2018-03-23 | 湖南省农业生物技术研究中心 | Schizophyllum commune XT 1 and its cultural method and application |
CN107828664B (en) * | 2017-10-25 | 2020-10-23 | 湖南省农业生物技术研究中心 | Schizophyllum commune XT-1 and cultivation method and application thereof |
CN109168961A (en) * | 2018-10-31 | 2019-01-11 | 芒市喜丰农业发展有限公司 | A kind of cultural method of white ginseng |
CN109220550A (en) * | 2018-10-31 | 2019-01-18 | 芒市喜丰农业发展有限公司 | A kind of method that white ginseng culture material waste residue recycles |
CN109348989A (en) * | 2018-12-03 | 2019-02-19 | 玉溪农业职业技术学院 | The efficient stable yields cultural method of white ginseng bacterium |
Also Published As
Publication number | Publication date |
---|---|
CN102090266B (en) | 2012-10-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102090266B (en) | High-efficient cultivation method of schizophyllum commune | |
CN104041330B (en) | Ganoderma tsugae imitates wild juggle cultivation method | |
CN103918475B (en) | The elegant precious method of mushroom bonsai type cultivation and the medium for cultivating elegant precious mushroom | |
CN101444170B (en) | Strain separation method of apricot ormer mushroom and cultivating method thereof | |
CN102786333B (en) | Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same | |
CN103907478B (en) | The method that flat mushroom bonsai type is cultivated and the substratum for cultivating white mushroom | |
CN104938211A (en) | Cultivation method for high yield and quality of mushroom | |
CN104557244A (en) | Cultivation medium for hericium erinaceus and cultivation method of hericium erinaceus | |
CN103891524A (en) | Potted lucid ganoderma cultivating method and culture medium for cultivating lucid ganoderma | |
CN102557792A (en) | Culture medium of beech mushrooms and culture method of beech mushrooms | |
CN104429611A (en) | Black fungi cultivation method | |
CN105237090A (en) | Cultivation method for phellinus igniarius strains from solid culture medium to liquid culture medium | |
CN103907477A (en) | Method and medium for cultivating mushrooms in potted landscape manner | |
KR100823541B1 (en) | Mushroom cultivation method | |
CN104855134A (en) | High-yield cultivation method for auricularia polytricha | |
CN106242824B (en) | A kind of planting almond abalone mushroom material and the implantation methods using planting material production pleurotus eryngii | |
CN106754409B (en) | Method for cultivating and planting holothuria leucorrhoeae | |
CN107216178A (en) | A kind of true pleurotus cornucopiae cultural method | |
KR101018145B1 (en) | Method for making of nutrient broth for cultivating Oyster mushrooms and nutrient broth for cultivating Oyster mushrooms made thereby | |
CN106416746A (en) | Cultivation method capable of increasing nutrition content of agrocybe cylindracea | |
KR100723068B1 (en) | Method for culturing flammulina velutipes including ginseng saponin | |
CN107912229A (en) | A kind of elegant precious mushroom compost and its method for producing elegant precious mushroom | |
CN103563653A (en) | Method for bottle cultivation of pleurotus cornucopiae | |
KR101634857B1 (en) | Cultivation method of shiitake mushrooms | |
Mizuno | Cultivation of the medicinal mushroom royal sun agaricus—Agaricus blazei Murr.(Agaricomycetideae) |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121031 Termination date: 20161205 |