CN105567576A - Liquid strain breeding method and field bionic cultivation method for bolete - Google Patents
Liquid strain breeding method and field bionic cultivation method for bolete Download PDFInfo
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Abstract
The invention provides a liquid strain breeding method for bolete, and further provides a field bionic cultivation method for the bolete. The liquid strain breeding method for the bolete comprises the steps that a first culture solution is firstly prepared from potatoes, wood bits, water, glucose, magnesium sulfate, monopotassium phosphate, peptone, vitamin B1, yeast and agar, and tissue at the joint of stipes and caps of the bolete is placed in the first culture solution to be cultured, and a mother strain is obtained; a second culture solution is prepared from potatoes, wood bits, corncob powder, water, glucose, magnesium sulfate, monopotassium phosphate, peptone and vitamin B1, the second culture solution is subpacked in a shake flask, the shake flask is inoculated with the mother strain, and a first liquid strain is obtained after culture. The field bionic cultivation method for the bolete mainly comprises the steps that firstly a fungus bag is obtained after the first liquid strain is subjected to inoculated culture, and then the fungus bag is buried in soil. The field bionic cultivation method for the bolete has the advantages of being short in production period, simple, low in infection rate and the like.
Description
Technical field
The present invention relates to the artificial breeding technique field of bolete, in particular to liquid spawn offspring breeding method and the land for growing field crops bionic cultivation method of a kind of bolete.
Background technology
Bolete is the general designation of the fungi such as Boletaceae and pinecone Boletaceae, is wild and edible mushroom mushroom, wherein except minority kind is poisonous or bitter and except can not eating, and the equal edible of most of kind.But be only limitted to wild mushroom at present, output is very limited, be difficult to meet the demand of people to bolete, so everybody is seeking a kind of method of artificial propagation bolete always.
The method of existing artificial propagation bolete not only complicated operation, production cycle long, and infection rate is high.
Summary of the invention
The object of the present invention is to provide the liquid spawn offspring breeding method of a kind of bolete, the liquid spawn offspring breeding method of described bolete has the advantages such as with short production cycle, method is simple, infection rate is low.
Another object of the present invention is the land for growing field crops bionic cultivation method providing a kind of bolete, the method have cost low, simple to operate, be convenient to the advantages such as popularization.
In order to realize above-mentioned purpose of the present invention, spy by the following technical solutions:
A liquid spawn offspring breeding method for bolete, comprises the following steps:
Plant making step: under sterile environment, get organizing of the stem of bolete and cap individually to add the first substratum and cultivate, mycelia stem and cap grown mixed culture under the environment of 26-30 DEG C, obtains female kind after the Hyphal form of stem and cap melts;
Wherein, by weight, the first substratum is made up of following component: 180-220 part potato, 180-220 part wood chip, 1600-2400 part water, 18-22 part glucose, 1.8-2.2 part magnesium sulfate, 2.8-3.2 part potassium primary phosphate, 4.8-5.2 part peptone, 0.25-1 part VITMAIN B1,1.05-1.35 part yeast tablet and 18-22 part agar;
Liquid spawn making step: under gnotobasis, access is female in the second nutrient solution plants, and carry out shaking table cultivation, the temperature that shaking table is cultivated is 24-26 DEG C, and the rotation frequency of shaking table is 350-500rpm, and shaking table is cultivated after 3-6 days and obtained first liquid bacterial classification;
Wherein, by weight, the second substratum is made up of following component: 180-220 part potato, 180-220 part wood chip, 1600-2400 part water, 18-22 part glucose, 1.8-2.2 part magnesium sulfate, 2.8-3.2 part potassium primary phosphate, 4.8-5.2 part peptone and 0.25-1 part VITMAIN B1.
Present invention also offers the land for growing field crops bionic cultivation method of a kind of bolete, comprise the following steps:
Bacterium bag making step: wheat, wood chip, soil, lime, gypsum, sugar, corn, dregs of beans are mixed to get mixture; In mixture, the weight percentage of each component is respectively: wheat 36-40%, wood chip 28-32%, native 8-12%, lime 1.8-2.2%, gypsum 0.8-1.2%, sugared 0.8-1.2%, corn 4-6%, dregs of beans 1.8-2.2%; Then mixture packed and carry out heat sterilization process, then first liquid bacterial classification is inoculated into mixture in an aseptic environment, then cultivating under 26-30 DEG C of condition and obtain bacterium bag in 40-50 days;
De-bag cultivating step: first excavate heatable adobe sleeping platform in cultivation area, be placed in heatable adobe sleeping platform after then bacterium bag being taken off bag, then banket in heatable adobe sleeping platform and cover bacterium bag, then water to cultivation area.
Compared with prior art, beneficial effect of the present invention is:
(1) the liquid spawn offspring breeding method of this bolete, with short production cycle, the time that solid spawn plants cultivar three tier structure kind from mother is 3 months, and prepares liquid spawn less than 20 days, the time of cultivating solid original seed or cultivar needs 90 days, and cultivates a set of liquid bacterial classification and only need 3-7 days.Make liquid spawn and substantially reduce the production of hybrid seeds time, need of production can be supplied in time.
(2) liquid spawn makes simple, and the making of solid spawn through planting original seed again to cultivar three tier structure kind process from mother, and will want replaced medium, and formality is numerous and diverse.The making of liquid spawn, as long as same substratum, cultivates bacterial classification out, both can be used as female kind and has used, and also can be used as the use of original seed, cultivar, decrease the replacing of substratum and the change of culture environment.
(3) inoculate easy, liquid spawn can with the inoculation of the various ways such as injection, spice, easy, quick, even, be suitable for mechanize, automatic mass production use.
(4) grow fast, the mycelium pellet Dou Shiyige organic centre of each liquid spawn, because mycelium pellet quantity is many, be evenly distributed, after inoculation, mycelia can grow rapidly, completes vegetative growth phase in the short period of time, avoids the generation of polluting and danger.Can find that the growing state of bacterium ball is wound around strong and weak to the mycelia of cultivar in advance.
(5) the liquid spawn offspring breeding method of this bolete is lower than solid spawn infection rate by about 50%.
(6) land for growing field crops bionic cultivation method reduces cost of investment, and processing ease is convenient, is more suitable for popularization, drives common people to build up the family fortunes.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below.
Fig. 1 is the distribution schematic diagram of cultivation area in booth.
Embodiment
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting the scope of the invention.Unreceipted actual conditions person in embodiment, the condition of conveniently conditioned disjunction manufacturers suggestion is carried out.Agents useful for same or the unreceipted production firm person of instrument, be and can buy by commercially available the conventional products obtained.
A liquid spawn offspring breeding method for bolete, comprises the following steps:
Female kind making step: 180-220 part potato and 180-220 part wood chip are used 800-1200 part poach 20-40min respectively, then filters and two kinds of filtrates are mixed to get the first mixing water.Conveniently filter, first can obtain potato water by filtering removal potato after 180-220 part potato 800-1200 part poach 20-40min, then 180-220 part wood chip is not installed with cloth bag, again the wood chip installed with cloth bag is put into and to take out after 800-1200 part water boils 20-40min and to obtain wood chip water, then obtain the first mixing water by after potato water and the mixing of wood chip water.
Then 18-22 part glucose, 1.8-2.2 part magnesium sulfate, 2.8-3.2 part potassium primary phosphate, 4.8-5.2 part peptone, 0.25-1 part VITMAIN B1,1.05-1.35 part yeast tablet and 18-22 part agar to be added in the first mixing water and boil 15-25min and obtain the first nutrient solution.Wherein VITMAIN B1 and sheet yeast tablet are all in the form of sheets, and 0.5 refers to half, and 1.5 refer to 1 and add half, the like.In addition, in order to make in the first nutrient solution each nutritive ingredient mixing evenly, after preferably the first nutrient solution being stirred use.
Again the first nutrient solution is divided in sterilising vessel and carries out heat sterilization process, then sterilising vessel left standstill and make the first nutrient solution cooled and solidified 3-5 days obtain the first substratum; Under sterile environment, get organizing of the stem of bolete and cap and individually add the first substratum and cultivate, by stem and mycelia mixed culture under the environment of 26-30 DEG C of growing of cap, obtain female kind after the Hyphal form of stem and cap melts.
As preferably, in female kind making step, bolete obtains by after the process of fresh beef liver bacterium, and treating processes is: pluck the fresh beef liver bacterium that growth is intact, strong, then fresh beef liver bacterium is put into encloses container or under 18-22 DEG C of condition, places 3-5 days with after adhesive plaster parcel.
As preferably, the condition of female heat sterilization process of planting in making step is: sterilizing 40-50min under pressure is 0.1-0.2Pa and temperature is the condition of 110-130 DEG C.
As preferably, sterilising vessel is test tube, is tilted by test tube and keep the angle of 15 ° to cool with horizontal plane after heat sterilization process.Test tube capacity is little, and the first cultivation liquid measure of every cuvette cartridge is few, is convenient to the germ in the first nutrient solution thoroughly to kill.Make test tube keep heeling condition to be the conventional means of this area, the liquid level area of the first nutrient solution can be increased, be conducive in vitro obtaining more femalely to plant single.Further, female growing state of planting can also be observed, select mother's kind that wherein growing way is good and purificate and rejuvenate.
Liquid spawn making step: by 180-220 part potato, 180-220 part wood chip 800-1200 part poach 20-40min, then filters and two kinds of filtrates is mixed to get the second mixing water; Similar with the making processes of the first mixing water, conveniently filter, first can obtain potato water by filtering removal potato after 180-220 part potato 800-1200 part poach 20-40min, then 180-220 part wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 800-1200 part water boils 20-40min and to obtain wood chip water, then obtain the second mixing water by after potato water and the mixing of wood chip water.
Again by 18-22 part glucose, 1.8-2.2 part magnesium sulfate, 2.8-3.2 part potassium primary phosphate, 4.8-5.2 part peptone, 0.25-1 part VITMAIN B1 to add in the second mixing water and boils 15-25min and obtains the second nutrient solution; Second nutrient solution is dispensed into shaking flask and carries out heat sterilization process, treat that the second nutrient solution is cooled to 24-26 DEG C, then access is female in an aseptic environment plants, again shaking flask is positioned over shaking table, the rotation frequency of shaking table is 350-500rpm, at 24-26 DEG C, obtain first liquid bacterial classification after wave and culture 12-16 days.
The making of existing solid spawn through planting original seed again to cultivar three tier structure kind process from mother, and will want replaced medium, and formality is numerous and diverse.And the making of liquid spawn of the present invention, as long as same substratum, cultivate bacterial classification out, namely female kind of gram conduct uses, and also can be used as original seed, the use of cultivation, decreases the replacing of substratum and the change of culture environment.
Liquid spawn grows fast, and the mycelium pellet Dou Shiyige organic centre of each liquid spawn, can grow rapidly because mycelium pellet quantity is many, be evenly distributed, inoculate rear mycelia, complete vegetative growth phase in the short period of time, avoids the occurrence and harm polluted.Can find that the growing state of bacterium ball is wound around strong and weak to the mycelia of cultivar in advance.In addition, various nutrient solution of the present invention always have employed best carbon-nitrogen ratio, adopts the liquid spawn offspring breeding method of bolete of the present invention, and the mycelia length of playing a ball game is consistent, it is vigorous to grow, growing way good, vitality good.
The growth that solids manufacture is planted is from the kind block vertical spread of inoculation, and the mycelia in whole culturing bottle (bag) differs 20 ~ 30 days from top to bottom cell age, and its vitality difference is larger.Each mycelia bead of liquid spawn is start the same time to be formed substantially, cell age is consistent, the incubation time of whole bacterial classification is very short, if use liquid spawn in mycelial growth exponential phase (incubation time was at 5 ~ 7 days), i.e. best seed stage, mycelia can very fast field planting, vigorous growth.
As preferably, the condition of the heat sterilization process in liquid spawn making step is: sterilizing 40-50min under pressure is 0.1-0.2Pa and temperature is the condition of 110-130 DEG C.
If produce on a small quantity, can directly make bacterium bag with first liquid bacterial classification and carry out land for growing field crops bionic cultivation.
A land for growing field crops bionic cultivation method for bolete, comprises step and the following steps of the liquid spawn offspring breeding method of bolete:
Bacterium bag making step: wheat, wood chip, soil, lime, gypsum, sugar, corn, dregs of beans are mixed to get mixture; In mixture, the weight percentage of each component is respectively: wheat 36-40%, wood chip 28-32%, native 8-12%, lime 1.8-2.2%, gypsum 0.8-1.2%, sugared 0.8-1.2%, corn 4-6%, dregs of beans 1.8-2.2%; Then mixture packed and carry out heat sterilization process, then first liquid bacterial classification is inoculated into mixture in an aseptic environment, then cultivating under 26-30 DEG C of condition and obtain bacterium bag in 40-50 days;
De-bag cultivating step: first excavate heatable adobe sleeping platform in cultivation area, be placed in heatable adobe sleeping platform after then bacterium bag being taken off bag, then banket in heatable adobe sleeping platform and cover bacterium bag, then water to cultivation area.
As preferably, the condition of the heat sterilization process in bacterium bag making step is: sterilizing 4-6h under pressure is 0.1-0.2Pa and temperature is the condition of 110-130 DEG C.
As preferably, the degree of depth of heatable adobe sleeping platform is 12-18cm, and the humidity of the soil of the cultivation area after watering to cultivation area is 75-95%, interval 3-5cm between adjacent bacterium bag, then bankets in heatable adobe sleeping platform and cover bacterium bag, and the soil thickness at the top of bacterium bag is 3-5cm.
As preferably, cultivation area is multiple and is distributed in booth, and the temperature in booth is 26-30 DEG C, and the atmospheric moisture in booth is 70-90%.
As preferably, booth projection is in the horizontal plane rectangle, the main aisle of the length direction along booth is provided with in booth, cultivation area is distributed in the both sides of main aisle, the width of main aisle is 1-1.5m, leaves the auxiliary passageway that width is 0.15-0.25m between the adjacent cultivation area of the same side of main aisle.
As preferably, booth is arch, the apogee distance ground 2-2.4m of booth, plastic house heat-insulating film, and plastic house transmittance is sunshade net or the puggaree of 25%-35%.
As preferred further, the transmittance of sunshade net or puggaree is 30%.
Bolete is liked growing under the environment of darkness, so need the irradiation dose being reduced sunlight by sunshade net or puggaree.Saying popular in industry be 7 second 3 points of sun best.
If industrialization is produced, also need to carry out enlarged culturing to first liquid bacterial classification, to increase the amount of liquid spawn.
As preferably, the liquid spawn offspring breeding method of bolete also comprises enlarged culturing step;
Enlarged culturing step is: first liquid bacterial classification is accessed fermentor tank in an aseptic environment, passes into sterile air and carry out air blowing to the 3rd nutrient solution in fermentor tank to stir in fermentor tank, cultivates and obtains second liquid bacterial classification after 3-6 days;
According to the ratio of the volume with fermentor tank, 3rd nutrient solution is made up of following component: wheat bran 40-60g/100L, vitaminB10 .06-0.2g/100L, yeast tablet 0.25-0.35g/100L, starch 1.2-1.8kg/100L, magnesium sulfate 0.8-1.2kg/100L, potassium primary phosphate 0.1-0.3kg/100L, glucose 0.4-0.6kg/100L, white sugar 0.4-0.6kg/100L, peptone 0.2-0.4kg/100L and edible oil 0.01-0.03kg/100L, and surplus is water.
Starch, magnesium sulfate, potassium primary phosphate, glucose, white sugar, peptone are all relevant to the volume preparing the 3rd nutrient solution obtained with the consumption of edible oil, the volume of usual fermentor tank is 100L, 200L, 400L and 500L, and correspondingly the volume of the 3rd nutrient solution is generally 100L, 200L, 400L and 500L.Starch, magnesium sulfate, potassium primary phosphate, glucose, white sugar, peptone and edible oil all can directly use.Under wheat bran is more special relative to other components, wheat bran needs to add poach 25-35min, then obtains wheat bran water through filtering removal wheat bran, then is added by wheat bran water in fermentor tank and the mixing of other components.Starch preferably first adds suitable quantity of water and soaks into the use of pasty state starch.The edible oil that edible oil can select rapeseed oil, soybean wet goods common.
When industrialization is produced, be make bacterium bag with second liquid bacterial classification, concrete making processes is with to make bacterium bag with first liquid bacterial classification just the same.
A land for growing field crops bionic cultivation method for bolete, comprises the following steps:
Bacterium bag making step: wheat, wood chip, soil, lime, gypsum, sugar, corn, dregs of beans are mixed to get mixture; In mixture, the weight percentage of each component is respectively: wheat 36-40%, wood chip 28-32%, native 8-12%, lime 1.8-2.2%, gypsum 0.8-1.2%, sugared 0.8-1.2%, corn 4-6%, dregs of beans 1.8-2.2%; Then mixture packed and carry out heat sterilization process, then second liquid bacterial classification is inoculated into mixture in an aseptic environment, then cultivating under 26-30 DEG C of condition and obtain bacterium bag in 40-50 days;
De-bag cultivating step: first excavate heatable adobe sleeping platform in cultivation area, be placed in heatable adobe sleeping platform after then bacterium bag being taken off bag, then banket in heatable adobe sleeping platform and cover bacterium bag, then water to cultivation area.
Embodiment 1
Present embodiments provide the liquid spawn offspring breeding method of a kind of bolete, comprise the following steps:
(1) female kind making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the first mixing water by after potato water and the mixing of wood chip water.
Then 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1,4 tablets of yeast tablets and 20g agar to be added in the first mixing water and boil 20min and obtain the first nutrient solution, for subsequent use after the first nutrient solution is stirred.Wherein the weight of every sheet VITMAIN B1 is 0.5g, and the weight of every sheet yeast tablet is 0.3g.
Again the first nutrient solution is divided in vitro, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C; Then test tube tried slant setting and keep the angle of 15 ° to cool under the environment in cool place with horizontal plane; First nutrient solution cooled and solidified obtained the first substratum after 4 days; Pluck the fresh beef liver bacterium that growth is intact, strong, then fresh beef liver bacterium is placed 4 days with after adhesive plaster parcel under 20 DEG C of conditions; Under sterile environment, get organizing of the stem of bolete and cap individually to add the first substratum and cultivate, after observing the sprouting 50% of stem and cap, mycelia stem and cap grown mixed culture under the environment of 28 DEG C, obtains female kind after the Hyphal form of stem and cap melts.
(2) liquid spawn making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the second mixing water by after potato water and the mixing of wood chip water;
Again by 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1 to add in the second mixing water and boils 20min and obtains the second nutrient solution; Shaking flask is dispensed into, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C after being stirred by second nutrient solution; Treat that the second nutrient solution is cooled to 25 DEG C, then access is female in an aseptic environment plants, then shaking flask is positioned over shaking table, and the rotation frequency of shaking table is 350rpm, and at 25 DEG C, wave and culture obtained first liquid bacterial classification after 14 days.
Embodiment 2
Present embodiments provide the liquid spawn offspring breeding method of a kind of bolete, comprise the following steps:
(1) female kind making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the first mixing water by after potato water and the mixing of wood chip water.
Then 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1,4 tablets of yeast tablets and 20g agar to be added in the first mixing water and boil 20min and obtain the first nutrient solution, for subsequent use after the first nutrient solution is stirred.Wherein the weight of every sheet VITMAIN B1 is 0.5g, and the weight of every sheet yeast tablet is 0.3g.
Again the first nutrient solution is divided in vitro, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C; Then test tube tried slant setting and keep the angle of 15 ° to cool under the environment in cool place with horizontal plane; First nutrient solution cooled and solidified obtained the first substratum after 4 days; Pluck the fresh beef liver bacterium that growth is intact, strong, then fresh beef liver bacterium is placed 4 days with after adhesive plaster parcel under 20 DEG C of conditions; Under sterile environment, get organizing of the stem of bolete and cap individually to add the first substratum and cultivate, after observing the sprouting 50% of stem and cap, mycelia stem and cap grown mixed culture under the environment of 28 DEG C, obtains female kind after the Hyphal form of stem and cap melts.
(2) liquid spawn making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the second mixing water by after potato water and the mixing of wood chip water;
Again by 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1 to add in the second mixing water and boils 20min and obtains the second nutrient solution; Shaking flask is dispensed into, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C after being stirred by second nutrient solution; Treat that the second nutrient solution is cooled to 25 DEG C, then access is female in an aseptic environment plants, then shaking flask is positioned over shaking table, and the rotation frequency of shaking table is 350rpm, and at 25 DEG C, wave and culture obtained first liquid bacterial classification after 14 days.
(3) enlarged culturing step is: be that the fermentor tank of 400L is cleaned and sterilizes that (process of concrete cleaning and sterilizing is by capacity, after fermentor tank cleans up, to each switch-valve carry out inspection errorless after, sky is carried out to fermentor tank and disappears, add water at fermentor tank skin, then heat, when internal pressure reaches 0.1Pa, design temperature, the time sets 35 minutes, open outer three switches connecting inner layer simultaneously, with water vapour, internal layer and pipeline are sterilized 35 minutes); Then wheat bran water is obtained by filtering after 200g wheat bran 1000mL poach 30min, wheat bran water, 1 VITMAIN B1,4 yeast tablets, 6kg pasty state starch, 4kg magnesium sulfate, 0.8kg potassium primary phosphate, 2kg glucose, 2kg white sugar, 0.8kg peptone and 0.08kg edible oils are added fermentor tank, in fermentor tank, add the maximum capacity scale place of sterilized water to fermentor tank again, after stirring, obtain 400L the 3rd nutrient solution; Heat sterilization is carried out to the 3rd nutrient solution, after the 3rd nutrient solution is cooled to 27 DEG C, first liquid bacterial classification is accessed fermentor tank in an aseptic environment, in fermentor tank, passes into sterile air and air blowing is carried out to the 3rd nutrient solution and stir, cultivate and obtain second liquid bacterial classification after 8 days.
Embodiment 3
Present embodiments provide the land for growing field crops bionic cultivation method of a kind of bolete, comprise the following steps:
(1) female kind making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the first mixing water by after potato water and the mixing of wood chip water.
Then 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1,4 tablets of yeast tablets and 20g agar to be added in the first mixing water and boil 20min and obtain the first nutrient solution, for subsequent use after the first nutrient solution is stirred.Wherein the weight of every sheet VITMAIN B1 is 0.5g, and the weight of every sheet yeast tablet is 0.3g.
Again the first nutrient solution is divided in vitro, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C; Then test tube tried slant setting and keep the angle of 15 ° to cool under the environment in cool place with horizontal plane; First nutrient solution cooled and solidified obtained the first substratum after 4 days; Pluck the fresh beef liver bacterium that growth is intact, strong, then fresh beef liver bacterium is placed 4 days with after adhesive plaster parcel under 20 DEG C of conditions; Under sterile environment, get organizing of the stem of bolete and cap individually to add the first substratum and cultivate, after observing the sprouting 50% of stem and cap, mycelia stem and cap grown mixed culture under the environment of 28 DEG C, obtains female kind after the Hyphal form of stem and cap melts.
(2) liquid spawn making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the second mixing water by after potato water and the mixing of wood chip water;
Again by 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1 to add in the second mixing water and boils 20min and obtains the second nutrient solution; Shaking flask is dispensed into, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C after being stirred by second nutrient solution; Treat that the second nutrient solution is cooled to 25 DEG C, then access is female in an aseptic environment plants, then shaking flask is positioned over shaking table, and the rotation frequency of shaking table is 350rpm, and at 25 DEG C, wave and culture obtained first liquid bacterial classification after 14 days.
(3) bacterium bag making step: wheat, wood chip, soil, lime, gypsum, sugar, corn, dregs of beans are mixed to get mixture; In mixture, the weight percentage of each component is respectively: wheat 38%, wood chip 30%, soil 10%, lime 2%, gypsum 1%, sugar 1%, corn 5%, dregs of beans 2%; Then by mixture pack, then under temperature 120 degree, pressure 0.15PA condition sterilizing 5h; With inoculating gun, first liquid bacterial classification is inoculated into mixture in an aseptic environment, then cultivates under 28 DEG C of conditions and obtain bacterium bag in 45 days.
(4) de-bag cultivating step: first excavate heatable adobe sleeping platform in cultivation area, the degree of depth of heatable adobe sleeping platform is 15cm's, then be placed in heatable adobe sleeping platform after bacterium bag being taken off bag, interval 4cm between adjacent bacterium bag, banket in heatable adobe sleeping platform again and cover bacterium bag, the soil thickness at the top of bacterium bag is 4cm, and the humidity of then watering to cultivation area to the soil of cultivation area is 75-95%.
As shown in Figure 1, cultivation area 1 is multiple and is distributed in booth, booth projection is in the horizontal plane rectangle, the main aisle 2 along the length direction of booth is provided with in booth, cultivation area 1 is distributed in the both sides of main aisle 2, the width of main aisle 2 is 1.2m, between the adjacent cultivation area 1 of the same side of main aisle 2, leave the auxiliary passageway 3 that width is 0.2m.Booth is arch, the apogee distance ground 2.2m of booth, plastic house heat-insulating film, and plastic house transmittance is sunshade net or the puggaree of 30%.Temperature in booth is 26-30 DEG C, and the atmospheric moisture in booth is 70-90%.
Embodiment 4
Present embodiments provide the land for growing field crops bionic cultivation method of a kind of bolete, comprise the following steps:
(1) female kind making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the first mixing water by after potato water and the mixing of wood chip water.
Then 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1,4 tablets of yeast tablets and 20g agar to be added in the first mixing water and boil 20min and obtain the first nutrient solution, for subsequent use after the first nutrient solution is stirred.Wherein the weight of every sheet VITMAIN B1 is 0.5g, and the weight of every sheet yeast tablet is 0.3g.
Again the first nutrient solution is divided in vitro, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C; Then test tube tried slant setting and keep the angle of 15 ° to cool under the environment in cool place with horizontal plane; First nutrient solution cooled and solidified obtained the first substratum after 4 days; Pluck the fresh beef liver bacterium that growth is intact, strong, then fresh beef liver bacterium is placed 4 days with after adhesive plaster parcel under 20 DEG C of conditions; Get organizing of the stem of bolete and cap individually to add the first substratum and cultivate, after observing the sprouting 50% of stem and cap, mycelia stem and cap grown mixed culture under the environment of 28 DEG C, obtains female kind after the Hyphal form of stem and cap melts.
(2) liquid spawn making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the second mixing water by after potato water and the mixing of wood chip water;
Again by 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1 to add in the second mixing water and boils 20min and obtains the second nutrient solution; Shaking flask is dispensed into, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C after being stirred by second nutrient solution; Treat that the second nutrient solution is cooled to 25 DEG C, then access is female in an aseptic environment plants, then shaking flask is positioned over shaking table, and the rotation frequency of shaking table is 350rpm, and at 25 DEG C, wave and culture obtained first liquid bacterial classification after 14 days.
(3) enlarged culturing step is: be that the fermentor tank of 400L is cleaned and sterilizes that (process of concrete cleaning and sterilizing is by capacity, after fermentor tank cleans up, to each switch-valve carry out inspection errorless after, sky is carried out to fermentor tank and disappears, add water at fermentor tank skin, then heat, when internal pressure reaches 0.1Pa, design temperature, the time sets 35 minutes, open outer three switches connecting inner layer simultaneously, with water vapour, internal layer and pipeline are sterilized 35 minutes); Then wheat bran water is obtained by filtering after 200g wheat bran 1000mL poach 30min, wheat bran water, 1 VITMAIN B1,4 yeast tablets, 6kg pasty state starch, 4kg magnesium sulfate, 0.8kg potassium primary phosphate, 2kg glucose, 2kg white sugar, 1.2kg peptone and 0.08kg edible oils are added fermentor tank, in fermentor tank, add the maximum capacity scale place of sterilized water to fermentor tank again, after stirring, obtain 400L the 3rd nutrient solution; Heat sterilization is carried out to the 3rd nutrient solution, after the 3rd nutrient solution is cooled to 27 DEG C, first liquid bacterial classification is accessed fermentor tank in an aseptic environment, in fermentor tank, passes into sterile air and air blowing is carried out to the 3rd nutrient solution and stir, cultivate and obtain second liquid bacterial classification after 8 days.
(4) bacterium bag making step: wheat, wood chip, soil, lime, gypsum, sugar, corn, dregs of beans are mixed to get mixture; In mixture, the weight percentage of each component is respectively: wheat 38%, wood chip 30%, soil 10%, lime 2%, gypsum 1%, sugar 1%, corn 5%, dregs of beans 2%; Then by mixture pack, then under temperature 120 degree, pressure 0.15PA condition sterilizing 5h; With inoculating gun, second liquid bacterial classification is inoculated into mixture in an aseptic environment, then cultivates under 28 DEG C of conditions and obtain bacterium bag in 45 days.
(5) de-bag cultivating step: first excavate heatable adobe sleeping platform in cultivation area, the degree of depth of heatable adobe sleeping platform is 15cm's, then be placed in heatable adobe sleeping platform after bacterium bag being taken off bag, interval 4cm between adjacent bacterium bag, banket in heatable adobe sleeping platform again and cover bacterium bag, the soil thickness at the top of bacterium bag is 4cm, and the humidity of then watering to cultivation area to the soil of cultivation area is 75-95%.
As shown in Figure 1, cultivation area 1 is multiple and is distributed in booth, booth projection is in the horizontal plane rectangle, the main aisle 2 along the length direction of booth is provided with in booth, cultivation area 1 is distributed in the both sides of main aisle 2, the width of main aisle 2 is 1.2m, between the adjacent cultivation area 1 of the same side of main aisle 2, leave the auxiliary passageway 3 that width is 0.2m.Booth is arch, the apogee distance ground 2.2m of booth, plastic house heat-insulating film, and plastic house transmittance is sunshade net or the puggaree of 30%.Temperature in booth is 26-30 DEG C, and the atmospheric moisture in booth is 70-90%.
Embodiment 5
Present embodiments provide the liquid spawn offspring breeding method of a kind of bolete, comprise the following steps:
(1) female kind making step: first obtain potato water by filtering removal potato after 180g potato 800mL poach 20min, then 180g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 800 water boil 20-40min and to obtain wood chip water, then obtain the first mixing water by after potato water and the mixing of wood chip water.
Then 18g glucose, 1.8g magnesium sulfate, 2.8g potassium primary phosphate, 4.8g peptone, 0.5 VITMAIN B1,3.5 tablets of yeast tablets and 18g agar to be added in the first mixing water and boil 15min and obtain the first nutrient solution, for subsequent use after the first nutrient solution is stirred.Wherein the weight of every sheet VITMAIN B1 is 0.5g, and the weight of every sheet yeast tablet is 0.3g.
Again the first nutrient solution is divided in vitro, sterilizing 50min under pressure is 0.15Pa and temperature is the condition of 110 DEG C; Then test tube tried slant setting and keep the angle of 15 ° to cool under the environment in cool place with horizontal plane; First nutrient solution cooled and solidified obtained the first substratum after 3 days; Pluck the fresh beef liver bacterium that growth is intact, strong, then fresh beef liver bacterium is placed 5 days with after adhesive plaster parcel under 18 DEG C of conditions; Under sterile environment, get organizing of the stem of bolete and cap individually to add the first substratum and cultivate, after observing the sprouting 50% of stem and cap, mycelia stem and cap grown mixed culture under the environment of 26 DEG C, obtains female kind after the Hyphal form of stem and cap melts.
(2) liquid spawn making step: first obtain potato water by filtering removal potato after 180g potato 800mL poach 20-40min, then 180g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 800mL water boils 20min and to obtain wood chip water, then obtain the second mixing water by after potato water and the mixing of wood chip water;
Again by 18g glucose, 1.8g magnesium sulfate, 2.8g potassium primary phosphate, 4.8g peptone, 0.5 VITMAIN B1 to add in the second mixing water and boils 15min and obtains the second nutrient solution; Second nutrient solution is dispensed into shaking flask, sterilizing 50min under pressure is 0.15Pa and temperature is the condition of 110 DEG C; Treat that the second nutrient solution is cooled to 24 DEG C, then access is female in an aseptic environment plants, then shaking flask is positioned over shaking table, and the rotation frequency of shaking table is 400rpm, and at 24 DEG C, wave and culture obtained first liquid bacterial classification after 16 days.
Embodiment 6
Present embodiments provide the liquid spawn offspring breeding method of a kind of bolete, comprise the following steps:
(1) female kind making step: first obtain potato water by filtering removal potato after 220g potato 1200mL poach 40min, then 220g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1200mL water boils 40min and to obtain wood chip water, then obtain the first mixing water by after potato water and the mixing of wood chip water.
Then 22g glucose, 2.2g magnesium sulfate, 3.2g potassium primary phosphate, 5.2g peptone, 1.5 VITMAIN B1,4.5 tablets of yeast tablets and 22g agar to be added in the first mixing water and boil 25min and obtain the first nutrient solution, for subsequent use after the first nutrient solution is stirred.Wherein the weight of every sheet VITMAIN B1 is 0.5g, and the weight of every sheet yeast tablet is 0.3g.
Again the first nutrient solution is divided in vitro, sterilizing 40min under pressure is 2Pa and temperature is the condition of 130 DEG C; Then test tube tried slant setting and keep the angle of 15 ° to cool under the environment in cool place with horizontal plane; First nutrient solution cooled and solidified obtained the first substratum after 5 days; Pluck the fresh beef liver bacterium that growth is intact, strong, then fresh beef liver bacterium is placed 3 days with after adhesive plaster parcel under 22 DEG C of conditions; Under sterile environment, get organizing of the stem of bolete and cap individually to add the first substratum and cultivate, after observing the sprouting 50% of stem and cap, mycelia stem and cap grown mixed culture under the environment of 30 DEG C, obtains female kind after the Hyphal form of stem and cap melts.
(2) liquid spawn making step: first obtain potato water by filtering removal potato after 220g potato 1200mL poach 40min, then 220g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1200mL water boils 40min and to obtain wood chip water, then obtain the second mixing water by after potato water and the mixing of wood chip water;
Again by 22g glucose, 2.2g magnesium sulfate, 3.2g potassium primary phosphate, 5.2g peptone, 1.5 VITMAIN B1 to add in the second mixing water and boil 25min and obtain the second nutrient solution; Second nutrient solution is dispensed into shaking flask, sterilizing 40min under pressure is 2Pa and temperature is the condition of 130 DEG C; Treat that the second nutrient solution is cooled to 26 DEG C, then access is female in an aseptic environment plants, then shaking flask is positioned over shaking table, and the rotation frequency of shaking table is 450rpm, and at 26 DEG C, wave and culture obtained first liquid bacterial classification after 12 days.
Embodiment 7
Present embodiments provide the liquid spawn offspring breeding method of a kind of bolete, comprise the following steps:
(1) female kind making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the first mixing water by after potato water and the mixing of wood chip water.
Then 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1,4 tablets of yeast tablets and 20g agar to be added in the first mixing water and boil 20min and obtain the first nutrient solution, for subsequent use after the first nutrient solution is stirred.Wherein the weight of every sheet VITMAIN B1 is 0.5g, and the weight of every sheet yeast tablet is 0.3g.
Again the first nutrient solution is divided in vitro, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C; Then test tube tried slant setting and keep the angle of 15 ° to cool under the environment in cool place with horizontal plane; First nutrient solution cooled and solidified obtained the first substratum after 4 days; Pluck the fresh beef liver bacterium that growth is intact, strong, then fresh beef liver bacterium is placed 4 days with after adhesive plaster parcel under 20 DEG C of conditions; Under sterile environment, get organizing of the stem of bolete and cap individually to add the first substratum and cultivate, after observing the sprouting 50% of stem and cap, mycelia stem and cap grown mixed culture under the environment of 28 DEG C, obtains female kind after the Hyphal form of stem and cap melts.
(2) liquid spawn making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the second mixing water by after potato water and the mixing of wood chip water;
Again by 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1 to add in the second mixing water and boils 20min and obtains the second nutrient solution; Shaking flask is dispensed into, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C after being stirred by second nutrient solution; Treat that the second nutrient solution is cooled to 25 DEG C, then access is female in an aseptic environment plants, then shaking flask is positioned over shaking table, and the rotation frequency of shaking table is 500rpm, and at 25 DEG C, wave and culture obtained first liquid bacterial classification after 14 days.
(3) enlarged culturing step is: be that the fermentor tank of 400L is cleaned and sterilizes that (process of concrete cleaning and sterilizing is by capacity, after fermentor tank cleans up, to each switch-valve carry out inspection errorless after, carry out sky to fermentor tank to disappear, add water at fermentor tank skin, then heat, when internal pressure reaches 0.1Pa, design temperature, time sets 35 minutes, open outer three switches connecting inner layer simultaneously, with water vapour, internal layer and pipeline are sterilized 35 minutes), wheat bran water is obtained by filtering after 220g wheat bran 1200mL poach 32min, by wheat bran water, 1.5 VITMAIN B1, 4.5 tablets of yeast tablets, 7.2kg pasty state starch, 4.8kg magnesium sulfate, 1.2kg potassium primary phosphate, 2.4kg glucose, 2.4kg white sugar, 1.2kg peptone and 0.12kg edible oil add fermentor tank, the maximum capacity scale place of sterilized water to fermentor tank is added again in fermentor tank, 400L the 3rd nutrient solution is obtained after stirring, heat sterilization is carried out to the 3rd nutrient solution, after the 3rd nutrient solution is cooled to 27 DEG C, first liquid bacterial classification is accessed fermentor tank in an aseptic environment, in fermentor tank, passes into sterile air and air blowing is carried out to the 3rd nutrient solution and stir, cultivate and obtain second liquid bacterial classification after 8 days.
Embodiment 8
Present embodiments provide the land for growing field crops bionic cultivation method of a kind of bolete, comprise the following steps:
(1) female kind making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the first mixing water by after potato water and the mixing of wood chip water.
Then 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1,4 tablets of yeast tablets and 20g agar to be added in the first mixing water and boil 20min and obtain the first nutrient solution, for subsequent use after the first nutrient solution is stirred.Wherein the weight of every sheet VITMAIN B1 is 0.5g, and the weight of every sheet yeast tablet is 0.3g.
Again the first nutrient solution is divided in vitro, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C; Then test tube tried slant setting and keep the angle of 15 ° to cool under the environment in cool place with horizontal plane; First nutrient solution cooled and solidified obtained the first substratum after 4 days; Pluck the fresh beef liver bacterium that growth is intact, strong, then fresh beef liver bacterium is placed 4 days with after adhesive plaster parcel under 20 DEG C of conditions; Under sterile environment, get organizing of the stem of bolete and cap individually to add the first substratum and cultivate, after observing the sprouting 50% of stem and cap, mycelia stem and cap grown mixed culture under the environment of 28 DEG C, obtains female kind after the Hyphal form of stem and cap melts.
(2) liquid spawn making step: first obtain potato water by filtering removal potato after 200g potato 1000mL poach 30min, then 200g wood chip cloth bag is installed, again the wood chip installed with cloth bag is put into and to take out after 1000mL water boils 30min and to obtain wood chip water, then obtain the second mixing water by after potato water and the mixing of wood chip water;
Again by 20g glucose, 2g magnesium sulfate, 3g potassium primary phosphate, 5g peptone, 1 VITMAIN B1 to add in the second mixing water and boils 20min and obtains the second nutrient solution; Shaking flask is dispensed into, sterilizing 45min under pressure is 0.15Pa and temperature is the condition of 120 DEG C after being stirred by second nutrient solution; Treat that the second nutrient solution is cooled to 25 DEG C, then access is female in an aseptic environment plants, then shaking flask is positioned over shaking table, and the rotation frequency of shaking table is 500rpm, and at 25 DEG C, wave and culture obtained first liquid bacterial classification after 5 days.
(3) enlarged culturing step is: be that the fermentor tank of 400L is cleaned and sterilizes that (process of concrete cleaning and sterilizing is by capacity, after fermentor tank cleans up, to each switch-valve carry out inspection errorless after, sky is carried out to fermentor tank and disappears, add water at fermentor tank skin, then heat, when internal pressure reaches 0.1Pa, design temperature, the time sets 35 minutes, open outer three switches connecting inner layer simultaneously, with water vapour, internal layer and pipeline are sterilized 35 minutes); Wheat bran water is obtained by filtering after 220g wheat bran 1200mL poach 32min, wheat bran water, 1.5 VITMAIN B1,4.5 yeast tablets, 7.2kg pasty state starch, 4.8kg magnesium sulfate, 1.2kg potassium primary phosphate, 2.4kg glucose, 2.4kg white sugar, 1.6kg peptone and 0.12kg edible oils are added fermentor tank, in fermentor tank, add the maximum capacity scale place of sterilized water to fermentor tank again, after stirring, obtain 400L the 3rd nutrient solution; Heat sterilization is carried out to the 3rd nutrient solution, after the 3rd nutrient solution is cooled to 27 DEG C, first liquid bacterial classification is accessed fermentor tank in an aseptic environment, in fermentor tank, passes into sterile air and air blowing is carried out to the 3rd nutrient solution and stir, cultivate and obtain second liquid bacterial classification after 7 days.
(4) bacterium bag making step: wheat, wood chip, soil, lime, gypsum, sugar, corn, dregs of beans are mixed to get mixture; In mixture, the weight percentage of each component is respectively: wheat 36%, wood chip 28%, soil 8%, lime 1.8%, gypsum 0.8%, sugar 0.8%, corn 4%, dregs of beans 1.8%; Then mixture is packed, then temperature be 120 DEG C, sterilizing 5h under pressure 0.15PA condition; With inoculating gun, second liquid bacterial classification is inoculated into mixture in an aseptic environment, then cultivates under 28 DEG C of conditions and obtain bacterium bag in 45 days.
(5) de-bag cultivating step: first excavate heatable adobe sleeping platform in cultivation area, the degree of depth of heatable adobe sleeping platform is 18cm's, then be placed in heatable adobe sleeping platform after bacterium bag being taken off bag, interval 5cm between adjacent bacterium bag, banket in heatable adobe sleeping platform again and cover bacterium bag, the soil thickness at the top of bacterium bag is 5cm, and the humidity of then watering to cultivation area to the soil of cultivation area is 75-95%.
As shown in Figure 1, cultivation area 1 is multiple and is distributed in booth, booth projection is in the horizontal plane rectangle, the main aisle 2 along the length direction of booth is provided with in booth, cultivation area 1 is distributed in the both sides of main aisle 2, the width of main aisle 2 is 1.5m, between the adjacent cultivation area 1 of the same side of main aisle 2, leave the auxiliary passageway 3 that width is 0.25m.Booth is arch, the apogee distance ground 2.4m of booth, plastic house heat-insulating film, and plastic house transmittance is sunshade net or the puggaree of 25%.Temperature in booth is 26-30 DEG C, and the atmospheric moisture in booth is 70-90%.
Although illustrate and describe the present invention with specific embodiment, however it will be appreciated that can to make when not deviating from the spirit and scope of the present invention many other change and amendment.Therefore, this means to comprise all such changes and modifications belonged in the scope of the invention in the following claims.
Claims (10)
1. a liquid spawn offspring breeding method for bolete, is characterized in that, comprise the following steps:
Female kind making step: under sterile environment, get organizing of the stem of bolete and cap individually to add the first substratum and cultivate, the mycelia mixed culture under the environment of 26-30 DEG C described stem and described cap grown, obtains female kind after the Hyphal form of described stem and described cap melts;
Wherein, by weight, described first substratum is made up of following component: 180-220 part potato, 180-220 part wood chip, 1600-2400 part water, 18-22 part glucose, 1.8-2.2 part magnesium sulfate, 2.8-3.2 part potassium primary phosphate, 4.8-5.2 part peptone, 0.25-1 part VITMAIN B1,1.05-1.35 part yeast tablet and 18-22 part agar;
Liquid spawn making step: access described mother under gnotobasis and plant in the second nutrient solution, carry out shaking table cultivation, the temperature that shaking table is cultivated is 24-26 DEG C, and shaking table is cultivated after 3-6 days and obtained first liquid bacterial classification;
Wherein, by weight, described second substratum is made up of following component: 180-220 part potato, 180-220 part wood chip, 1600-2400 part water, 18-22 part glucose, 1.8-2.2 part magnesium sulfate, 2.8-3.2 part potassium primary phosphate, 4.8-5.2 part peptone and 0.25-1 part VITMAIN B1.
2. the liquid spawn offspring breeding method of bolete according to claim 1, is characterized in that, the liquid spawn offspring breeding method of bolete also comprises enlarged culturing step;
Described enlarged culturing step is: described first liquid bacterial classification is accessed fermentor tank in an aseptic environment, passes into sterile air and carry out air blowing to the 3rd nutrient solution in described fermentor tank to stir in described fermentor tank, cultivates and obtains second liquid bacterial classification after 3-6 days;
According to the ratio of the volume with described fermentor tank, described 3rd nutrient solution is made up of following component: wheat bran 40-60g/100L, vitaminB10 .06-0.2g/100L, yeast tablet 0.25-0.35g/100L, starch 1.2-1.8kg/100L, magnesium sulfate 0.8-1.2kg/100L, potassium primary phosphate 0.1-0.3kg/100L, glucose 0.4-0.6kg/100L, white sugar 0.4-0.6kg/100L, peptone 0.2-0.4kg/100L and edible oil 0.01-0.03kg/100L, and surplus is water.
3. the liquid spawn offspring breeding method of bolete according to claim 1, it is characterized in that, described mother plants in making step, described bolete obtains by after the process of fresh beef liver bacterium, treating processes is: pluck the fresh beef liver bacterium that growth is intact, strong, then fresh beef liver bacterium is put into encloses container or under 18-22 DEG C of condition, places 3-5 days with after adhesive plaster parcel.
4. the liquid spawn offspring breeding method of bolete according to claim 1, it is characterized in that, the condition of the heat sterilization process in described liquid spawn making step and in female kind making step is: sterilizing 40-50min under pressure is 0.1-0.2Pa and temperature is the condition of 110-130 DEG C.
5. the liquid spawn offspring breeding method of bolete according to claim 1, is characterized in that, described first substratum is slant medium.
6. a land for growing field crops bionic cultivation method for bolete, is characterized in that, comprises the step as described in any one of claim 1-5 and following steps:
Bacterium bag making step: wheat, wood chip, soil, lime, gypsum, sugar, corn, dregs of beans are mixed to get mixture; In described mixture, the weight percentage of each component is respectively: described wheat 36-40%, described wood chip 28-32%, described native 8-12%, described lime 1.8-2.2%, described gypsum 0.8-1.2%, described sugared 0.8-1.2%, described corn 4-6%, described dregs of beans 1.8-2.2%; Then described mixture packed and carry out heat sterilization process, again first liquid bacterial classification as claimed in claim 1 or second liquid bacterial classification as claimed in claim 2 are inoculated into described mixture in an aseptic environment, then cultivate under 26-30 DEG C of condition and obtain bacterium bag in 40-50 days;
De-bag cultivating step: first excavate heatable adobe sleeping platform in cultivation area, be placed in heatable adobe sleeping platform after then described bacterium bag being taken off bag, then banket in heatable adobe sleeping platform and cover described bacterium bag, then water to cultivation area.
7. the land for growing field crops bionic cultivation method of bolete according to claim 6, it is characterized in that, the degree of depth of described heatable adobe sleeping platform is 12-18cm's, the humidity of the soil of the cultivation area after watering to described cultivation area is 75-95%, interval 3-5cm between adjacent bacterium bag, banket in heatable adobe sleeping platform and cover described bacterium bag, the soil thickness at the top of described bacterium bag is 3-5cm.
8. the land for growing field crops bionic cultivation method of bolete according to claim 6, is characterized in that, described cultivation area is multiple and is distributed in booth, and the temperature in booth is 26-30 DEG C, and the atmospheric moisture in booth is 70-90%.
9. the land for growing field crops bionic cultivation method of bolete according to claim 8, it is characterized in that, the projection in the horizontal plane of described booth is rectangle, the main aisle of the length direction along booth is provided with in described booth, described cultivation area is distributed in the both sides of described main aisle, the width of described main aisle is 1-1.5m, between the adjacent described cultivation area of the same side of described main aisle, leave the auxiliary passageway that width is 0.15-0.25m.
10. the land for growing field crops bionic cultivation method of bolete according to claim 9, it is characterized in that, described booth is arch, the apogee distance ground 2-2.4m of described booth, described plastic house heat-insulating film, described plastic house transmittance is sunshade net or the puggaree of 25%-35%.
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| CN107641601A (en) * | 2017-10-20 | 2018-01-30 | 翔天农业开发集团股份有限公司 | A kind of liquid fermentation strain domestication method |
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| CN113348966A (en) * | 2021-06-07 | 2021-09-07 | 云南省热带作物科学研究所 | Culture medium and culture method for Boletus zhonghuasheng liquid strain |
| CN115104481A (en) * | 2021-12-31 | 2022-09-27 | 林礼 | Method for liquid culture of Huang cluster bacterium strain |
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