CN104126551A - Method for breeding aphidius gifuensis ashmead through floating tobacco seedlings - Google Patents
Method for breeding aphidius gifuensis ashmead through floating tobacco seedlings Download PDFInfo
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Abstract
The invention discloses a method for breeding aphidius gifuensis ashmead through floating tobacco seedlings, particularly relates to a method for breeding aphidius gifuensis ashmead through floating tobacco seedlings, which is realized through breeding aphids by cultivating uniform, robust tobacco seedling with proper density and inoculating mummified aphids under net covering condition, and belongs to the technical field of insect breeding and biological control of injurious insects. According to the technical system for breeding aphidius gifuensis ashmead efficiently through floating tobacco seedlings, innovation is achieved in the three aphidius gifuensis ashmead breeding steps including host plant cultivation, host myzus persicae breeding and aphidius gifuensis ashmead breeding, the aphidius gifuensis ashmead breeding cycle is shortened to be two months, and the number of mummified aphids and parasitic aphids existing in each square meter can reach over one hundred and twenty thousand; the steps such as tobacco plant transplantation and cultivation are omitted, management is easy and convenient, and a small number of manpower and material resources are needed. By the adoption of the method for breeding aphidius gifuensis ashmead through floating tobacco seedlings, the aphidius gifuensis ashmead can be bred by fully utilizing existing breeding facilities, aphidius gifuensis ashmead breeding facility investment is reduced, and aphidius gifuensis ashmead breeding cost is reduced. The method is tested in large-scale aphidius gifuensis ashmead breeding work, the time efficiency, the unit-area efficiency, the management efficiency, the aphidius gifuensis ashmead breeding facility utilization rate and other aspects of large-scale aphidius gifuensis ashmead breeding are improved comprehensively, the aphidius gifuensis ashmead breeding cost is reduced greatly, and the method for breeding aphidius gifuensis ashmead is adopted by most tobacco enterprises in China.
Description
Technical field
The neat stalwartness of a kind of floating cigarette seedling breeding aphidius gifuensis method, particularly cultivation of the present invention, the numerous aphid of cigarette seedling that density is suitable connect stiff aphid seedling in good time, and the numerous honeybee method of floating cigarette seedling under cover net condition, belongs to insect raising and biological control of insect pests technical field.
Background technology
Denomination of invention " high-efficiency floating seedling Aphidius gifuensis propagation method " Chinese patent (application number or the patent No.: 201110209370.2) that applicant once declared, this Patent Application Publication is utilized seedling raising greenhouse, the field after flue-cured tobacco transplanting, vacated grow seedlings hut and nursery pond thereof, floats dish carry out alternate row seeding floating seedlings with 162 holes or 200 holes; In the time that having 3～4 true leaves, cigarette seedling connects aphid, numerous aphid; In the time that aphid amount reaches 300～350 heads/plant, by honeybee: aphid is than the honeybee amount that connects of 1:100, in required one-tenth honeybee access cover net; Under 20～30 DEG C of conditions, through 10～15 days, stiff aphid formed, becomes bee eclosion in a large number.
But through actual the applying of 3 years, applicant found: former declaring in patent adopts 3～4 leaf 1 heart cigarette seedlings to connect aphid, numerous aphid, and the problem of existence is that cigarette seedling is less, along with the rapid growth of aphid amount, tobacco seedling growth is bad, causes a large amount of parasitic aphids to be fled from or death, and the stiff aphid amount of formation is less; Be in the time that aphid amount reaches 300～350 heads/plant, to access into honeybee the honeybee opportunity of connecing in former patent return, the problem existing is in the numerous honeybee production of scale reality, easily cause damage when being gathered into honeybee, become honeybee easily dead, the honeybee that is connected into efficient lower, consequently connecing after honeybee can not abundant parasitic cigarette aphid, and the stiff aphid amount of formation is less.
Summary of the invention
The object of the invention is to improve the problem existing in former patent application, a kind of floating cigarette seedling breeding aphidius gifuensis method is originally provided, further improve numerous honeybee efficiency.
A kind of Floating seedling breeding of the present invention aphidius gifuensis method step is as follows:
(1) cultivate strong sprout: in the time that cigarette seedling has 5 true leaves, straight snips one hypo-tobacco leaf, but large short little, to cultivate the healthy and strong cigarette seedling that growth is neat, Group Consistency is good, density is suitable;
(2) connect the numerous aphid of aphid: while waiting step cigarette seedling to grow to 6 true leaves, kind of an aphid is connect and guided on cigarette seedling, allow cigarette aphid spread voluntarily breeding, it is high that the healthy and strong cigarette seedling forming after Leaf cutting carries aphid amount, continues numerous aphid time long, and the numerous aphid amount of unit are is high;
(3) connect the numerous honeybee of honeybee: in the time that cigarette aphid quantity reaches approximately 150 heads/plant, by honeybee: aphid is than the honeybee amount that connects for 1:50, in the stiff aphid of required aphidius gifuensis and parasitic aphid (stiff aphid seedling) access cover net, appoint into that honeybee sprouts wings voluntarily, parasitic cigarette aphid, breed the aphidius gifuensis making new advances;
(4) live cigarette seedling with the numerous honeybee cover of 60 order guard in time after connecing aphid, form space limited, sealing, until numerous honeybee is finished, prevent from connecing the intrusion of aphidius gifuensis and other insect after aphid, affect the breeding of cigarette aphid, prevent from connecing fleeing from of aphidius gifuensis after honeybee, improve parasitic effects.
Described (1) step is utilized existing seedling growth greenhouse, adopt long × wide × high dish that floats for 66.5cm × 34cm × 5.5cm, 162 cave specifications, cultivate numerous honeybee cigarette seedling by the requirement of growing seedlings of floating cigarette seedling, without filling the gaps with seedlings, in the time of cigarette seedling 5 leaf 1 heart, by floating seedlings requirement straight snips one hypo-tobacco leaf, but large short little, the healthy and strong cigarette seedling that cultivation growth is neatly, Group Consistency is good; The seedling natural death of part cigarette and Leaf cutting in seedling raising process, reduced gap between the seedling of cigarette seedling density, formation and increased permeability, met cigarette aphid and breed the needs to subenvironment between seedling.
Described (2) step is in the time that cigarette seedling has 6 leaf 1 heart, kind of aphid leaf is laid in to (cigarette aphid raw leaf back contact cigarette seedling) on cigarette seedling, appoint cigarette aphid to spread voluntarily, in the time of approximately 10 heads/plant of average aphid amounts on cigarette seedling, kind of aphid leaf is changed on new cigarette seedling, healthy and strong cigarette seedling nutritional sufficiency, numerous aphid amount is high, the duration is long.
(3) described step is in the time that cigarette aphid quantity reaches approximately 150 heads/plant, by honeybee: aphid, than the honeybee amount that connects for 1:50, in the stiff aphid of required aphidius gifuensis and parasitic aphid (stiff aphid seedling) access cover net, becomes honeybee to continue to sprout wings, continuous parasitic cigarette aphid, and bee yield is high.
Described (4) step is lived cigarette seedling with the numerous honeybee cover of 60 order guard after connecing aphid in time, until numerous honeybee is finished, forms space limited, sealing, prevent from connecing the intrusion of aphidius gifuensis and other insect after aphid, affect the breeding of cigarette aphid, prevent from connecing fleeing from of aphidius gifuensis after honeybee, improve parasitic effects.
One of innovative point of the present invention is by Leaf cutting, but large short little, tobacco seedling growth is neat, healthy and strong, it is high and lasting that individual plant carries aphid amount, and the effective numerous aphid cigarette seedling quantity of unit are increases, and Group Consistency is good, numerous aphid amount of unit are is high, realizes the lasting effect of efficient numerous aphid.
The key technology of efficient breeding aphidius gifuensis is that Fast-propagation goes out a large amount of host aphids in unit are.Breed a large amount of host aphids, the one, the fertility of host aphid self, as single female farrowing amount, generation time etc.; The 2nd, there are enough host plants and sufficient nutrition thereof; The 3rd, need suitable conditions of growth and development, as suitable temperature, humidity, illumination and living space.In numerous aphid work, people often only notice the quantity of host plant, ignore the density of host plant, the impact that living space grows on aphid.If cigarette seedling density is too high, a little less than seedling, be unfavorable for the breeding of cigarette aphid, not only aphid amount is lower, also can produce a large amount of alatae.In former patent return, notice cigarette seedling density issue, effectively improved numerous aphid amount, but the impact of the lasting effect of having ignored efficient numerous aphid on later stage breeding effect.
The numerous honeybee method of the present invention, in the time that cigarette seedling has 5 true leaves, is carried out a straight snips processing, and one of its effect is the growth that can promote cigarette shoot root, stem, and the cigarette seedling of more not leaf-cutting is neat, healthy and strong, and Group Consistency is good; In the time of cigarette seedling 6 leaf 1 heart, connect aphid, numerous aphid, the aphid amount of carrying of cigarette seedling is also higher, and lasting effect is also longer, connects after aphid the 12nd day, and aphid amount reaches nearly 500 heads/plant, keeps more than 700 heads/plant within the time of 15th～21 days, is conducive to the lasting parasitism of aphidius gifuensis.Two of its effect is to have played to press down large short little effect, in the time that cigarette seedling has 5 true leaves, carries out a hypo-tobacco leaf straight snips processing, and effective numerous aphid cigarette seedling quantity in unit are is increased.Three of its effect is to have rejected partial blade after Leaf cutting, makes cigarette seedling density suitable, and the intensity of illumination between Miao Yumiao increases, and has played the effect that interlacing is grown seedlings, the cigarette aphid breeding of carrying out after being conducive to.And without the cigarette seedling of Leaf cutting, connecing after aphid the 18th day, aphid amount only reaches nearly 420 heads/plant, thereafter quantity fast-descending.
Two of innovative point of the present invention is that stiff aphid and parasitic aphid the raw stiff aphid seedling that cries on cigarette seedling using stiff aphid and parasitic aphid (stiff aphid seedling) as planting honeybee, becomes that honeybee continues to sprout wings voluntarily, parasitic cigarette aphid, has avoided artificially collecting the damage to one-tenth honeybee, has improved parasitic efficiency.
Be in the time that aphid amount reaches 300～350 heads/plant, to access into honeybee the honeybee opportunity of connecing in former patent return, the problem one existing is in the numerous honeybee production of scale reality, easily cause damage when being gathered into honeybee, become honeybee easily dead, the honeybee that is connected into efficient lower, consequently connecing after honeybee can not abundant parasitic cigarette aphid; In addition while connecing honeybee higher, the cigarette seedling of aphid amount a little less than, cause parasitic aphid to be fled from or dead, the stiff aphid amount of formation is less, this be also we original to declare last patent unexpected.
The application is in the time that aphid amount reaches approximately 150 heads/plant, by honeybee: aphid connects honeybee than the honeybee amount that connects for 1:50, and the kind honeybee of access is stiff aphid seedling, existing stiff aphid, also has parasitic aphid, becomes that honeybee continues to sprout wings, parasitic cigarette aphid connecing after honeybee, avoid artificially collecting the damage to becoming honeybee, improved parasitic efficiency.
Three of innovative point of the present invention is " pressure the connects honeybee " methods that kept former patent application, efficient parasitic cigarette aphid.
Cover cigarette seedling with 60 order fly nets, form space limited, sealing, connect the intrusion that prevents aphidius gifuensis and other insect after aphid, affect the propagation of cigarette aphid; After connecing honeybee, prevent fleeing from of aphidius gifuensis.The parasitism of aphidius gifuensis to cigarette aphid, except the parasitic ability of self, is also subject to the impact of activity space and host plant density.The space of seedling raising greenhouse is very large, and aphidius gifuensis free diffusing is difficult to form the efficient parasitism to cigarette aphid.So, cover cigarette seedling with the little shed that 60 order fly nets are made, make aphidius gifuensis in space limited, sealing, find cigarette aphid parasitism, become one " pressure connects honeybee method ", effectively improve parasitic rate, form more stiff aphid.
Four of innovative point of the present invention is that numerous honeybee comprehensive benefit further improves.
Floating seedling breeding aphidius gifuensis technical system after the above-mentioned improvement of the present invention, cultivating host plant, connect aphid-numerous aphid, connect honeybee-all innovate in 3 numerous honeybee links of numerous honeybee, not only keeping in former patent application advantages such as " utilize the numerous honeybee of existing culture facility, numerous honeybee cycle is short, cost is low ", also further improved bee yield---the bee yield of every square metre of nursery pond reaches more than 120,000.
Brief description of the drawings
Fig. 1 be numerous aphid amount in two kinds of cigarette seedling processing mode processes (head/plant) change curve.
2013 to 2014, in the Fan Feng base, Muller branch company east wind Triangle of applicant unit, in conjunction with the numerous honeybee work of scale, carry out the leaf-cutting numerous honeybee of floating cigarette seedling and the numerous honeybee of non-leaf-cutting cigarette seedling, form a set of new Floating seedling breeding aphidius gifuensis method, further improved numerous honeybee efficiency.Step is as follows:
(1) cultivate strong sprout: in the time that cigarette seedling has 5 true leaves, straight snips one hypo-tobacco leaf, produces and press down large short little effect, after cultivating, obtains the healthy and strong cigarette seedling that growth is neat, Group Consistency is good, density is suitable;
(2) connect the numerous aphid of aphid: in the time that the cigarette seedling of the 1st step has grown to 6 true leaves, kind of an aphid is connect and guided on cigarette seedling, allow cigarette aphid spread voluntarily breeding, it is high that the growth that forms after Leaf cutting is neat, Group Consistency good, the suitable healthy and strong cigarette seedling of density carries aphid amount, continue numerous aphid time long, the numerous aphid amount of unit are is high;
(3) connect the numerous honeybee of honeybee: in the time that cigarette aphid quantity reaches approximately 150 heads/plant, by honeybee: aphid is than the honeybee amount that connects for 1:50, in the stiff aphid of required aphidius gifuensis and parasitic aphid (stiff aphid seedling) access cover net, appoint into that honeybee sprouts wings voluntarily, parasitic cigarette aphid, numerous aphidius gifuensis making new advances;
(4) connect after aphid and live cigarette seedling with the numerous honeybee cover of 60 order guard in time, until numerous honeybee is finished, form space limited, that seal, prevent from connecing the intrusion of aphidius gifuensis and other insect after aphid, affect the breeding of cigarette aphid, prevent from connecing fleeing from of aphidius gifuensis after honeybee, improve parasitic effects.
(1) step is utilized existing seedling growth greenhouse, adopt long × wide × high dish that floats for 66.5cm × 34cm × 5.5cm, 162 cave specifications, cultivate numerous honeybee cigarette seedling by the requirement of growing seedlings of floating cigarette seedling, without filling the gaps with seedlings, in the time of cigarette seedling 5 leaf 1 heart, by floating seedlings requirement straight snips one hypo-tobacco leaf, but large short little, the healthy and strong cigarette seedling that cultivation growth is neatly, Group Consistency is good; The seedling natural death of part cigarette and Leaf cutting in seedling raising process, reduced gap between the seedling of cigarette seedling density, formation and increased permeability, met cigarette aphid and breed the needs to subenvironment between seedling.
(2) step is in the time that cigarette seedling has 6 leaf 1 heart, kind of aphid leaf is laid in to (cigarette aphid raw leaf back contact cigarette seedling) on cigarette seedling, appoint cigarette aphid to spread voluntarily, in the time of approximately 10 heads/plant of average aphid amounts on cigarette seedling, kind of aphid leaf is changed on new cigarette seedling, healthy and strong cigarette seedling nutritional sufficiency, numerous aphid amount is high, the duration is long.
(3) step is in the time that cigarette aphid quantity reaches approximately 150 heads/plant, by honeybee: aphid, than the honeybee amount that connects for 1:50, in the stiff aphid of required aphidius gifuensis and parasitic aphid (stiff aphid seedling) access cover net, becomes honeybee to continue to sprout wings, continuous parasitic cigarette aphid, and bee yield is high.
(4) step is lived cigarette seedling with the numerous honeybee cover of 60 order guard after connecing aphid in time, until numerous honeybee is finished, forms space limited, sealing, prevent from connecing the intrusion of aphidius gifuensis and other insect after aphid, affect the breeding of cigarette aphid, prevent from connecing fleeing from of aphidius gifuensis after honeybee, improve parasitic effects.
embodiment 1(the breeding effect comparisons of two kinds of cigarette seedling processing modes)
(1) cultivate cigarette seedling
In same numerous honeybee canopy, with length × wide × height be 66.5cm × 34cm × 5.5cm, 162 cave specifications floating disc, by floating seedlings requirement seeding and seedling raising.
(2) connect aphid, numerous aphid
Process I (former method): in the time of 3～4 true leaves of cigarette seedling, kind of an aphid leaf is laid on cigarette seedling, leaf back is downward, appoint cigarette aphid to spread voluntarily, in the time on average having approximately 10 heads/plant of cigarette aphids on cigarette seedling, kind of aphid leaf is changed on new cigarette seedling, cover with 60 object fly nets in time after connecing aphid.
Process II (the inventive method): in the time that cigarette seedling has 5 true leaves, by leaf of floating tobacco seedling leaf shearing requirement straight snips, after leaf-cutting 3 days, when cigarette seedling forms the 6th true leaf, kind of an aphid leaf is laid on cigarette seedling, leaf back is downward, appoint cigarette aphid to spread voluntarily, in the time on average having approximately 10 heads/plant of cigarette aphids on cigarette seedling, kind of aphid leaf is changed on new cigarette seedling, cover with 60 object fly nets in time after connecing aphid.
(3) numerous aphid management
While connecing aphid, pack up sunshade net, strengthen illumination; The management expectancy of floating cigarette seedling carries out water and fertilizer management routinely, with cigarette seedling keep vigorous growth, blade is light green is advisable; Make temperature of shed remain on 20 DEG C～30 DEG C by the folding and unfolding of horticultural covering film and sunshade net as far as possible.
(4) investigation statistics
Adopt parallel sampling method, connect after aphid the 3rd day, the 6th day, the 9th day, the 12nd day, the 15th day, the 18th day, the 21st day, extract altogether 10 dish cigarette seedlings at two ends and the middle part of nursery pond, the each extraction 10 strain cigarette seedlings of every dish, statistics cigarette aphid quantity.
(5) numerous aphid effect
Result shows, while connecing aphid, the average cigarette seedling of two kinds of processing is all 135 strains, and 3～4 leaf 1 heart cigarette seedlings are when connecing aphid 18 days, and after average cigarette seedling number is 90.4 strains, 419.4 heads/plant of the highest aphid amounts, coil approximately 37914 of aphid amounts.6 leaf 1 heart cigarette seedlings through Leaf cutting are connecing 15～21 days after aphid, and average cigarette seedling number is 102.67 strains, 736.8 heads/plant of the highest aphid amounts, approximately 75647 of dish aphid amounts.6 leaf 1 heart cigarette seedlings are connecing the 12nd day aphid amount of aphid higher than 3～4 leaf 1 heart cigarette seedlings maximum aphid amount of the 18th day; Meanwhile, the aphid amount of 6 leaf 1 heart cigarette seedlings continues to keep higher level, and the aphid amount of 3～4 leaf 1 heart cigarette seedlings declines rapidly.In table 1 and Fig. 1.
embodiment 2(the breeding effect comparisons of two kinds of cigarette seedling processing modes)
(1) cultivate cigarette seedling
In same numerous honeybee canopy, with length × wide × height be 66.5cm × 34cm × 5.5cm, 162 cave specifications floating disc, by floating seedlings requirement seeding and seedling raising.
(2) numerous aphid, numerous honeybee
Process I (former method): in the time of 3～4 true leaves of cigarette seedling, kind of an aphid leaf is laid on cigarette seedling, leaf back is downward, appoint cigarette aphid to spread voluntarily, in the time on average having approximately 10 heads/plant of cigarette aphids on cigarette seedling, kind of aphid leaf is changed on new cigarette seedling, cover with 60 object fly nets in time after connecing aphid.While connecing approximately 260 heads/plant of 15 days average aphid amounts of aphid, by honeybee: aphid is accessed into respectively honeybee or stiff aphid seedling than 1:50,1:100, cover and float dish with 60 order fly nets after connecing honeybee, appoint into honeybee and find cigarette aphid parasitism.
Process II (the inventive method): in the time that cigarette seedling has 5 true leaves, by leaf of floating tobacco seedling leaf shearing requirement straight snips, after leaf-cutting 3 days, when cigarette seedling forms the 6th true leaf, kind of an aphid leaf is laid on cigarette seedling, leaf back is downward, appoint cigarette aphid to spread voluntarily, in the time on average having approximately 10 heads/plant of cigarette aphids on cigarette seedling, kind of aphid leaf is changed on new cigarette seedling, cover with 60 object fly nets in time after connecing aphid.While connecing approximately 150 of 9 days average aphid amounts of aphid/dish, by honeybee: aphid is accessed into respectively honeybee or stiff aphid seedling than 1:50,1:100, cover and float dish with 60 order fly nets after connecing honeybee, appoint into honeybee searching cigarette aphid parasitism.
(3) numerous aphid, bee management
While connecing aphid, pack up sunshade net, strengthen illumination; The management expectancy of floating cigarette seedling carries out water and fertilizer management routinely, with cigarette seedling keep vigorous growth, blade is light green is advisable; Make temperature of shed remain on 20 DEG C～30 DEG C by the folding and unfolding of horticultural covering film and sunshade net as far as possible.
(4) investigation statistics
Adopt parallel sampling method, connect after honeybee the 15th day, the 18th day, the 21st day, the 24th day, extract altogether 10 dish cigarette seedlings at two ends and the middle part of nursery pond, the each extraction 10 strain cigarette seedlings of every dish, statistics cigarette seedling number, stiff aphid quantity, parasitic aphid quantity.
(5) breeding effect
Result demonstration, with 3～4 numerous aphids of true leaf cigarette seedling, the stiff aphid amount and the total parasitic number that respectively connect honeybee processing all kept higher quantity at 15th～18 days that connect after honeybee, taking stiff aphid seedling as kind of a honeybee, the highest by the stiff aphid amount of honeybee: aphid=1:50 and total parasitic number, in table 2.
Through 6 numerous aphids of true leaf cigarette seedling of cigarette of Leaf cutting, respectively connect the total parasitic number of honeybee processing in the higher quantity that keeps for 15th～21 days connecing after honeybee, respectively connect the stiff aphid amount of honeybee processing in the higher quantity that keeps for 21st～24 days connecing after honeybee, taking stiff aphid seedling as kind of a honeybee, the highest by the stiff aphid amount of honeybee: aphid=1:50 and total parasitic number; From maximum bee yield, the stiff aphid amount of 6 true leaf cigarette seedlings of Leaf cutting, total parasitic number respectively compared with 3～4 leaf heart cigarette seedlings high 14958/dish, 8649/dish, in table 2.
From seeding and seedling raising to numerous stiff aphid seedling that goes out to be suitable for bulk storage, numerous honeybee cycle when both stiff aphid or parasitic sum reach maximum, 3～4 leaf heart cigarette seedlings be 58～61 days, 6 leaf heart cigarette seedlings be 62～65 days, only differ 4 day time, in table 3.
With 3～4 numerous honeybees of true leaf cigarette seedling, a little less than cigarette seedling, to cover at numerous honeybee later stage cigarette seedling, yellow leaf phenomenon is serious, the parasitic aphid of part can not form stiff aphid, and effective numerous honeybee rate reduces.6 numerous honeybees of true leaf cigarette seedling of Leaf cutting, until while being suitable for putting honeybee, cigarette seedling is stood upright all the time, blade keeps green, are all almost stiff aphid and parasitic aphid.
The present invention makes full use of the numerous honeybee of existing culture facility, and numerous honeybee remarkable benefit improves, and land for growing field crops plants cigarette and grows seedlings and often conflict mutually in time with a large amount of numerous honeybees work, and common practices is to build separately numerous honeybee canopy, and its expense accounts for the over half of numerous honeybee cost first.Adopt Floating seedling numerous honeybee required time only 2 months, can plant cigarette according to land for growing field crops and grow seedlings the time difference of growing seedlings with numerous honeybee, make full use of the seedling growth greenhouse vacated after flue-cured tobacco transplanting and nutrient solution numerous honeybee of growing seedlings, can reduce to a great extent numerous honeybee cost.As numerous honeybee in seedling raising greenhouse, only need on nursery pond, add a cover the hut of growing seedlings of 60 order fly nets; As the numerous honeybee in hut of growing seedlings, only fly net need be changed into 60 orders.Cultivation cigarette can continue to use after growing seedlings and need not changing sterilization with the nutrient solution of crossing; After emerging, according to tobacco seedling growth situation, topdress by the management expectancy of floating seedlings.
Claims (5)
1. a floating cigarette seedling breeding aphidius gifuensis method, is characterized in that breeding aphidius gifuensis step as follows:
(1) cultivate strong sprout: in the time that cigarette seedling has 5 true leaves, straight snips one hypo-tobacco leaf, but large short little, to cultivate the healthy and strong cigarette seedling that growth is neat, Group Consistency is good, density is suitable;
(2) connect the numerous aphid of aphid: while waiting step cigarette seedling to grow to 6 true leaves, kind of an aphid is connect and guided on cigarette seedling, allow cigarette aphid spread voluntarily breeding, it is high that the healthy and strong cigarette seedling forming after Leaf cutting carries aphid amount, continues numerous aphid time long, and the numerous aphid amount of unit are is high;
(3) connect the numerous honeybee of honeybee: in the time that cigarette aphid quantity reaches approximately 150 heads/plant, by honeybee: aphid is than the honeybee amount that connects for 1:50, in the stiff aphid of required aphidius gifuensis and parasitic aphid (stiff aphid seedling) access cover net, appoint into that honeybee sprouts wings voluntarily, parasitic cigarette aphid, breed the aphidius gifuensis making new advances;
(4) live cigarette seedling with the numerous honeybee cover of 60 order guard in time after connecing aphid, form space limited, sealing, until numerous honeybee is finished, prevent from connecing the intrusion of aphidius gifuensis and other insect after aphid, affect the breeding of cigarette aphid, prevent from connecing fleeing from of aphidius gifuensis after honeybee, improve parasitic effects.
2. floating cigarette seedling breeding aphidius gifuensis method according to claim 1, it is characterized in that (1) step utilizes existing seedling growth greenhouse, adopt long × wide × high dish that floats for 66.5cm × 34cm × 5.5cm, 162 cave specifications, cultivate numerous honeybee cigarette seedling by the requirement of growing seedlings of floating cigarette seedling, without filling the gaps with seedlings, in the time of cigarette seedling 5 leaf 1 heart, by floating seedlings requirement straight snips one hypo-tobacco leaf, but large short little, the healthy and strong cigarette seedling that cultivation growth is neatly, Group Consistency is good; The seedling natural death of part cigarette and Leaf cutting in seedling raising process, reduced gap between the seedling of cigarette seedling density, formation and increased permeability, met cigarette aphid and breed the needs to subenvironment between seedling.
3. according to the floating cigarette seedling breeding aphidius gifuensis method described in claim 1, it is characterized in that (2) step is in the time that cigarette seedling has 6 leaf 1 heart, kind of aphid leaf is laid in to (cigarette aphid raw leaf back contact cigarette seedling) on cigarette seedling, appoint cigarette aphid to spread voluntarily, in the time of approximately 10 heads/plant of average aphid amounts on cigarette seedling, kind of aphid leaf is changed on new cigarette seedling, healthy and strong cigarette seedling nutritional sufficiency, numerous aphid amount is high, the duration is long.
4. floating cigarette seedling breeding aphidius gifuensis method according to claim 1, it is characterized in that (3) step is in the time that cigarette aphid quantity reaches approximately 150 heads/plant, by honeybee: aphid is than the honeybee amount that connects for 1:50, in the stiff aphid of required aphidius gifuensis and parasitic aphid (stiff aphid seedling) access cover net, become honeybee to continue to sprout wings, continuous parasitic cigarette aphid, bee yield is high.
5. floating cigarette seedling breeding aphidius gifuensis method according to claim 1, it is characterized in that living cigarette seedling with the numerous honeybee cover of 60 order guard in time after (4) step connects aphid, until numerous honeybee is finished, form space limited, sealing, prevent from connecing the intrusion of aphidius gifuensis and other insect after aphid, affect the breeding of cigarette aphid, prevent from connecing fleeing from of aphidius gifuensis after honeybee, improve parasitic effects.
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| CN104938262A (en) * | 2015-06-29 | 2015-09-30 | 宁波市农业科学研究院 | Aphid biological control method with barley and Sitobion avenae as storage carriers |
| CN105104311A (en) * | 2015-09-25 | 2015-12-02 | 黔南州烟草公司瓮安县分公司 | Method for breeding aphidius gifuensis ashmeads through alternanthera philoxeroides |
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| CN105211008A (en) * | 2015-11-09 | 2016-01-06 | 云南省烟草公司玉溪市公司 | A kind of method improving dish aphid parasite reproductive efficiency |
| CN105941341A (en) * | 2016-05-30 | 2016-09-21 | 贵州省烟草公司毕节市公司 | A method for rapid aphid propagation in an aphidius gifuensis breeding process |
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| CN107455341A (en) * | 2017-08-01 | 2017-12-12 | 中国烟草总公司福建省公司 | A kind of aphidius gifuensis mating system of regulation and control host crop growth |
| CN107371764A (en) * | 2017-09-08 | 2017-11-24 | 云南省烟草公司楚雄州公司 | A kind of method that wet tobacco seedlings breeding cigarette aphid and aphidius gifuensis are floated using regeneration |
| CN108419758A (en) * | 2018-01-30 | 2018-08-21 | 西南林业大学 | A kind of efficient numerous bee method for improving aphid parasite deadlock aphid and forming equilibrium degree |
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