CN104111256A - Method for detecting content of soil alkali-hydrolyzable nitrogen - Google Patents

Method for detecting content of soil alkali-hydrolyzable nitrogen Download PDF

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Publication number
CN104111256A
CN104111256A CN201410317310.6A CN201410317310A CN104111256A CN 104111256 A CN104111256 A CN 104111256A CN 201410317310 A CN201410317310 A CN 201410317310A CN 104111256 A CN104111256 A CN 104111256A
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diffusion boat
alkaline hydrolysis
soil
sample
plastics
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严君
韩晓增
郭爱雪
赵家煦
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Northeast Institute of Geography and Agroecology of CAS
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Northeast Institute of Geography and Agroecology of CAS
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Abstract

A method of measurement soil alkaline hydrolysis nitrogen content, it is related to the method for measuring soil alkaline hydrolysis nitrogen content. The purpose of invention is to solve the alkaline hydrolysis diffusion method of existing analysis soil alkaline hydrolysis nitrogen content in the presence of batch quantity analysis is unable to, and increases workload, the problem that error is big and detection data is not accurate enough. Step: one, preparation is equipped with the plastics diffusion boat sample to soil alkaline hydrolysis; Two, alkaline hydrolysis; Three, blank control sample is prepared; Four, formula is utilized Calculate soil alkaline hydrolysis nitrogen content W1, W2, W3, W4 and W5 in 5 samples; Five, it is averaged and obtains soil alkaline hydrolysis nitrogen content. The present invention can get a kind of method for measuring soil alkaline hydrolysis nitrogen content.

Description

A kind of assay method of soil alkali-hydrolyzable nitrogen content
Technical field
The present invention relates to measure the method for soil alkali-hydrolyzable nitrogen content.
Background technology
Soil alkali-hydrolyzable nitrogen content energy reflect soil is nitrogen supply situation at no distant date, it is one of the project that must analyze of current national fertilization compositions based on earth measurement project, the analytic approach of current existing routine is alkaline hydrolysis diffusion method, glue spreading method is wherein to spread upon diffusion boat mistress edge after adopting glass bar to dip in alkaline glue, this method of operating seriously restricts the efficiency of batch quantity analysis, increase workload, easily make some small differences out in the cold simultaneously, make the data of detection not accurate enough, and glass diffusion boat is fragile when cleaning diffusion boat.
Summary of the invention
The alkaline hydrolysis diffusion method that the object of the invention is to solve existing analysis soil alkali-hydrolyzable nitrogen content exists can not batch quantity analysis, increases workload, and error is large and detect the not accurate enough problem of data, and a kind of assay method of soil alkali-hydrolyzable nitrogen content is provided.
An assay method for soil alkali-hydrolyzable nitrogen content, specifically completes according to the following steps:
One, the plastics diffusion boat sample for the treatment of soil alkaline hydrolysis is equipped with in preparation: 1., first take air-dry pedotheque and 0.2g ferrous sulphate powder that 2.00g crosses 2mm aperture sieve; Then air-dry pedotheque and the 0.2g ferrous sulphate powder of the 2.00g taking being crossed to 2mm aperture sieve mix, then are laid in the mistress of plastics diffusion boat; To the interior chamber of plastics diffusion boat, adding 2mL boric acid concentration is boric acid-mixed indicator of 20g/L, re-using silica gel bowl cover covers above plastics diffusion boat, opening silica gel bowl cover makes plastics diffusion boat mistress expose a slit, the sodium hydroxide solution that is 1mol/L by 10mL concentration again joins in plastics diffusion boat mistress by slit, then covers silica gel bowl cover; Obtain being equipped with the plastics diffusion boat sample 1 for the treatment of soil alkaline hydrolysis; 2., repeating step one 1. 4 times, obtain being equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 2, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 3, be equipped with and treat the plastics diffusion boat sample 4 of soil alkaline hydrolysis and the plastics diffusion boat sample 5 for the treatment of soil alkaline hydrolysis is housed;
Two, alkaline hydrolysis: 1., by treat soil alkaline hydrolysis plastics diffusion boat sample 1, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 2, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 3, be equipped with and treat the plastics diffusion boat sample 4 of soil alkaline hydrolysis and be equipped with to treat that the plastics diffusion boat sample 5 of soil alkaline hydrolysis packs in diffusion boat supporting plate, then diffusion boat supporting plate is placed on culturing rack;
2., culturing rack is put into temperature is the constant temperature oven of 40 ℃, alkaline hydrolysis diffusion 24h; Re-use sulfuric acid standard solution respectively 5 of titration treat the ammonia in boric acid-mixed indicator that in the plastics diffusion boat sample of soil alkaline hydrolysis, chamber mesoboric acid concentration is 20g/L; When the boric acid concentration boric acid-mixed indicator that is 20g/L becomes blush from blue look, be titration end-point, stop titration and record the volume V that consumes sulfuric acid standard solution in titration 1, V 2, V 3, V 4and V 5;
Three, prepare blank sample: get a clean plastics diffusion boat, to the interior chamber of plastics diffusion boat, adding 2mL boric acid concentration is again boric acid-mixed indicator of 20g/L, re-using silica gel bowl cover covers above plastics diffusion boat, opening silica gel bowl cover makes plastics diffusion boat mistress expose a slit, the sodium hydroxide solution that is 1mol/L by 10mL concentration again joins in plastics diffusion boat mistress by slit, then covers silica gel bowl cover; Again plastics diffusion boat is put into temperature and is the constant temperature oven of 40 ℃, alkaline hydrolysis diffusion 24h; Re-use the ammonia in boric acid-mixed indicator that in sulfuric acid standard solution titration plastics diffusion boat, chamber boric acid concentration is 20g/L; When the boric acid concentration boric acid-mixed indicator that is 20g/L becomes blush from blue look, be titration end-point, stop the volume V that titration record consume sulfuric acid standard solution 0;
Four, utilize following computing formula to calculate respectively soil alkali-hydrolyzable nitrogen content W in 5 samples 1, W 2, W 3, W 4and W 5;
W i = C × ( V - V 0 ) × 14 m × 1000 ;
In above-mentioned formula:
W i---soil alkali-hydrolyzable nitrogen content in sample, unit is (mg/kg);
C---the concentration of sulfuric acid standard solution, unit is mole every liter (mol/L);
V---in titration, consume the volume of sulfuric acid standard solution, unit is milliliter (mL);
V 0---titration empty control sample consumes the volume of sulfuric acid standard solution, and unit is milliliter (mL);
14---the molal weight of nitrogen-atoms, unit is for gram often rubbing (g/mol);
M---the quality of wind desiceted soil earth sample in sample, unit is (g);
Five, W step 4 being obtained 1, W 2, W 3, W 4and W 5average and obtain soil alkali-hydrolyzable nitrogen content.
Advantage of the present invention:
One, the assay method of a kind of soil alkaline hydrolyzable nitrogen provided by the invention, by reduced the cost of experiment with plastics diffusion boat, and greatly reduce the breakage of cleaning process, with silica gel bowl cover, substituted glass cover slide and made testing process simplicity, reduce use and the impact on the result of experiment of experiment reagent, guaranteed the preci-sion and accuracy of data;
Two, the testing result by standard pedotheque proves, data measured of the present invention is all within critical field, and that existing method records number is higher compared with standard value, the relative standard deviation that the present invention simultaneously measures the method for soil alkali-hydrolyzable nitrogen content is 4.52% lower than existing methods relative standard deviation 5.65%.
The present invention can obtain a kind of method of measuring soil alkali-hydrolyzable nitrogen content.
Accompanying drawing explanation
Fig. 1 is that plastics diffusion boat and the silica gel described in embodiment one covers the section structure schematic diagram being combined with; In Fig. 1,1 is silica gel bowl cover, and 2 is plastics diffusion boat;
Fig. 2 is the diffusion boat carriage structure schematic diagram described in embodiment one, described on diffusion boat planker, arrange 5 with Fig. 1 in the groove that matches of plastics diffusion boat external diameter, plastics diffusion boat packs in groove;
Fig. 3 is the side structure schematic diagram of the culturing rack described in embodiment one;
Fig. 4 is the plan structure schematic diagram that the diffusion boat planker described in embodiment one is combined with culturing rack; In Fig. 4,1 is culturing rack, the 2nd, and diffusion boat planker, 3 is plastics diffusion boat.
Embodiment
Embodiment one: in conjunction with Fig. 1 to Fig. 4, present embodiment is a kind of method of measuring soil alkali-hydrolyzable nitrogen content, specifically completes according to the following steps:
An assay method for soil alkali-hydrolyzable nitrogen content, specifically completes according to the following steps:
One, the plastics diffusion boat sample for the treatment of soil alkaline hydrolysis is equipped with in preparation: 1., first take air-dry pedotheque and 0.2g ferrous sulphate powder that 2.00g crosses 2mm aperture sieve; Then air-dry pedotheque and the 0.2g ferrous sulphate powder of the 2.00g taking being crossed to 2mm aperture sieve mix, then are laid in the mistress of plastics diffusion boat; To the interior chamber of plastics diffusion boat, adding 2mL boric acid concentration is boric acid-mixed indicator of 20g/L, re-using silica gel bowl cover covers above plastics diffusion boat, opening silica gel bowl cover makes plastics diffusion boat mistress expose a slit, the sodium hydroxide solution that is 1mol/L by 10mL concentration again joins in plastics diffusion boat mistress by slit, then covers silica gel bowl cover; Obtain being equipped with the plastics diffusion boat sample 1 for the treatment of soil alkaline hydrolysis; 2., repeating step one 1. 4 times, obtain being equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 2, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 3, be equipped with and treat the plastics diffusion boat sample 4 of soil alkaline hydrolysis and the plastics diffusion boat sample 5 for the treatment of soil alkaline hydrolysis is housed;
Two, alkaline hydrolysis: 1., by treat soil alkaline hydrolysis plastics diffusion boat sample 1, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 2, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 3, be equipped with and treat the plastics diffusion boat sample 4 of soil alkaline hydrolysis and be equipped with to treat that the plastics diffusion boat sample 5 of soil alkaline hydrolysis packs in diffusion boat supporting plate, then diffusion boat supporting plate is placed on culturing rack;
2., culturing rack is put into temperature is the constant temperature oven of 40 ℃, alkaline hydrolysis diffusion 24h; Re-use sulfuric acid standard solution respectively 5 of titration treat the ammonia in boric acid-mixed indicator that in the plastics diffusion boat sample of soil alkaline hydrolysis, chamber mesoboric acid concentration is 20g/L; When the boric acid concentration boric acid-mixed indicator that is 20g/L becomes blush from blue look, be titration end-point, stop titration and record the volume V that consumes sulfuric acid standard solution in titration 1, V 2, V 3, V 4and V 5;
Three, prepare blank sample: get a clean plastics diffusion boat, to the interior chamber of plastics diffusion boat, adding 2mL boric acid concentration is again boric acid-mixed indicator of 20g/L, re-using silica gel bowl cover covers above plastics diffusion boat, opening silica gel bowl cover makes plastics diffusion boat mistress expose a slit, the sodium hydroxide solution that is 1mol/L by 10mL concentration again joins in plastics diffusion boat mistress by slit, then covers silica gel bowl cover; Again plastics diffusion boat is put into temperature and is the constant temperature oven of 40 ℃, alkaline hydrolysis diffusion 24h; Re-use the ammonia in boric acid-mixed indicator that in sulfuric acid standard solution titration plastics diffusion boat, chamber boric acid concentration is 20g/L; When the boric acid concentration boric acid-mixed indicator that is 20g/L becomes blush from blue look, be titration end-point, stop the volume V that titration record consume sulfuric acid standard solution 0;
Four, utilize following computing formula to calculate respectively soil alkali-hydrolyzable nitrogen content W in 5 samples 1, W 2, W 3, W 4and W 5;
W i = C × ( V - V 0 ) × 14 m × 1000 ;
In above-mentioned formula:
W i---soil alkali-hydrolyzable nitrogen content in sample, unit is (mg/kg);
C---the concentration of sulfuric acid standard solution, unit is mole every liter (mol/L);
V---in titration, consume the volume of sulfuric acid standard solution, unit is milliliter (mL);
V 0---titration empty control sample consumes the volume of sulfuric acid standard solution, and unit is milliliter (mL);
14---the molal weight of nitrogen-atoms, unit is for gram often rubbing (g/mol);
M---the quality of wind desiceted soil earth sample in sample, unit is (g);
Five, W step 4 being obtained 1, W 2, W 3, W 4and W 5average and obtain soil alkali-hydrolyzable nitrogen content.
Fig. 1 is that plastics diffusion boat and the silica gel described in embodiment one covers the section structure schematic diagram being combined with; In Fig. 1,1 is silica gel bowl cover, and 2 is plastics diffusion boat;
Fig. 2 is the diffusion boat carriage structure schematic diagram described in embodiment one, described on diffusion boat planker, arrange 5 with Fig. 1 in the groove that matches of plastics diffusion boat external diameter, plastics diffusion boat packs in groove;
Fig. 3 is the side structure schematic diagram of the culturing rack described in embodiment one;
Fig. 4 is the plan structure schematic diagram that the diffusion boat planker described in embodiment one is combined with culturing rack; In Fig. 4,1 is culturing rack, the 2nd, and diffusion boat planker, 3 is plastics diffusion boat.
The principle of the advantage of present embodiment and mensuration soil alkali-hydrolyzable nitrogen content method:
The assay method of a kind of soil alkaline hydrolyzable nitrogen that, present embodiment provides, by reduced the cost of experiment with plastics diffusion boat, and greatly reduce the breakage of cleaning process, with silica gel bowl cover, substituted glass cover slide and made testing process simplicity, reduce use and the impact on the result of experiment of experiment reagent, guaranteed the preci-sion and accuracy of data;
Two, the testing result by standard pedotheque proves, present embodiment data measured is all within critical field, and that existing method records number is higher compared with standard value, to measure the relative standard deviation of the method for soil alkali-hydrolyzable nitrogen content be 4.52% lower than existing methods relative standard deviation 5.65% to present embodiment simultaneously.
This embodiment can obtain a kind of assay method of soil alkali-hydrolyzable nitrogen content.
Embodiment two: present embodiment and embodiment one difference are: the compound method of boric acid-mixed indicator that the boric acid concentration of step 1 described in is 1. 20g/L is: 20g boric acid is dissolved in the hot distilled water of 950mL, naturally after cooling, the mixed indicator that adds 20mL, after fully mixing, the sodium hydroxide solution that dropping concentration is 0.1mol/L, pH value to boric acid-mixed indicator is 4.5, then boric acid-mixed indicator is diluted to 1L; Wherein, described mixed indicator be the bromcresol green of 0.09g and 0.066g methyl red to be dissolved in 100mL massfraction be in 95% ethanolic solution, formulated.Other steps are identical with embodiment one.
Embodiment three: present embodiment and one of embodiment one or two difference are: the concentration of the sulfuric acid standard solution of step 2 described in is 2. 0.01mol/L.Other steps are identical with embodiment one or two.
Embodiment four: one of present embodiment and embodiment one to three difference is: the concentration of the sulfuric acid standard solution described in step 3 is 0.01mol/L.Other steps are identical with embodiment one to three.
Adopt following verification experimental verification beneficial effect of the present invention:
Test one: a kind of assay method of soil alkali-hydrolyzable nitrogen content specifically completes according to the following steps:
One, the plastics diffusion boat sample for the treatment of soil alkaline hydrolysis is equipped with in preparation: 1., first by GBW07414, take air-dry pedotheque and the 0.2g ferrous sulphate powder that standard pedotheque 2.00g crosses 2mm aperture sieve; Then air-dry pedotheque and the 0.2g ferrous sulphate powder of the 2.00g taking being crossed to 2mm aperture sieve mix, then are laid in the mistress of plastics diffusion boat; To the interior chamber of plastics diffusion boat, adding 2mL boric acid concentration is boric acid-mixed indicator of 20g/L, re-using silica gel bowl cover covers above plastics diffusion boat, opening silica gel bowl cover makes plastics diffusion boat mistress expose a slit, the sodium hydroxide solution that is 1mol/L by 10mL concentration again joins in plastics diffusion boat mistress by slit, then covers silica gel bowl cover; Obtain being equipped with the plastics diffusion boat sample 1 for the treatment of soil alkaline hydrolysis; 2., repeating step 1. 4 times, obtain being equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 2, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 3, be equipped with and treat the plastics diffusion boat sample 4 of soil alkaline hydrolysis and the plastics diffusion boat sample 5 for the treatment of soil alkaline hydrolysis is housed;
Two, alkaline hydrolysis: 1., by treat soil alkaline hydrolysis plastics diffusion boat sample 1, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 2, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 3, be equipped with and treat the plastics diffusion boat sample 4 of soil alkaline hydrolysis and be equipped with to treat that the plastics diffusion boat sample 5 of soil alkaline hydrolysis packs in diffusion boat supporting plate, then diffusion boat supporting plate is placed on culturing rack;
2., culturing rack is put into temperature is the constant temperature oven of 40 ℃, alkaline hydrolysis diffusion 24h; Re-use sulfuric acid standard solution respectively 5 of titration treat the ammonia in boric acid-mixed indicator that in the plastics diffusion boat sample of soil alkaline hydrolysis, chamber mesoboric acid concentration is 20g/L; When the boric acid concentration boric acid-mixed indicator that is 20g/L becomes blush from blue look, be titration end-point, stop titration and record the volume V that consumes sulfuric acid standard solution in titration 1, V 2, V 3, V 4and V 5;
Three, prepare blank sample: get a clean plastics diffusion boat, to the interior chamber of plastics diffusion boat, adding 2mL boric acid concentration is again boric acid-mixed indicator of 20g/L, re-using silica gel bowl cover covers above plastics diffusion boat, opening silica gel bowl cover makes plastics diffusion boat mistress expose a slit, the sodium hydroxide solution that is 1mol/L by 10mL concentration again joins in plastics diffusion boat mistress by slit, then covers silica gel bowl cover; Again plastics diffusion boat is put into temperature and is the constant temperature oven of 40 ℃, alkaline hydrolysis diffusion 24h; Re-use the ammonia in boric acid-mixed indicator that in sulfuric acid standard solution titration plastics diffusion boat, chamber boric acid concentration is 20g/L; When the boric acid concentration boric acid-mixed indicator that is 20g/L becomes blush from blue look, be titration end-point, stop the volume V that titration record consume sulfuric acid standard solution 0;
Four, utilize following computing formula to calculate respectively soil alkali-hydrolyzable nitrogen content W in 5 samples 1, W 2, W 3, W 4and W 5;
W i = C × ( V - V 0 ) × 14 m × 1000 ;
In above-mentioned formula:
W i---soil alkali-hydrolyzable nitrogen content in sample, unit is (mg/kg);
C---the concentration of sulfuric acid standard solution, unit is mole every liter (mol/L);
V---in titration, consume the volume of sulfuric acid standard solution, unit is milliliter (mL);
V 0---the volume of titration empty control sample consumption consumption sulfuric acid standard solution, unit is milliliter (mL);
14---the molal weight of nitrogen-atoms, unit is for gram often rubbing (g/mol);
M---the quality of wind desiceted soil earth sample in sample, unit is (g);
Five, W step 4 being obtained 1, W 2, W 3, W 4and W 5average and obtain soil alkali-hydrolyzable nitrogen content.
Test boric acid-mixed indicator that the boric acid concentration described in a step 2 is 20g/L;
The concentration of testing the sulfuric acid standard solution of a step 2 described in is 4. 0.01mol/L;
The concentration of testing the sulfuric acid standard solution described in a step 3 is 0.01mol/L.
By W 1, W 2, W 3, W 4, W 5and mean value lists in table 1, table 1 is the testing result of test one;
Table 1
Sample number 1 2 3 4 5 Mean value Standard value Relative standard deviation
Measured value mg/kg 66 74 72 68 70 70 66±8 4.52％
As known from Table 1, mean value is 70mg/kg, and soil alkali-hydrolyzable nitrogen content is 70mg/kg; The standard value of test is 66 ± 8, and relative standard deviation is 4.52%.
Contrast test: the assay method of existing soil alkali-hydrolyzable nitrogen content, specifically completes according to the following steps:
One, the diffusion boat sample for the treatment of soil alkaline hydrolysis is equipped with in preparation: 1., first by GBW07414, take air-dry pedotheque and the 0.2g ferrous sulphate powder that standard pedotheque 2.00g crosses 2mm aperture sieve, then air-dry pedotheque and the 0.2g ferrous sulphate powder of the 2.00g taking being crossed to 2mm aperture sieve mix, then are laid in the mistress of diffusion boat, to the interior chamber of diffusion boat, adding 2mL boric acid concentration is boric acid-mixed indicator of 20g/L, then at diffusion boat mistress edge, coat Arabic gum, cover frosted glass, rotation, make diffusion boat and frosted glass completely bonding, turn-off frosted glass on one side again, make diffusion boat mistress expose a slit, the sodium hydroxide solution that is 1mol/L by 10mL concentration again joins in diffusion boat mistress by slit, make again frosted glass cover tightly diffusion boat, re-use bungee circle tight, frosted glass is fixed on diffusion boat, shake diffusion boat, sodium hydroxide solution can not be mixed with the boric acid-mixed indicator of interior chamber, sodium hydroxide solution is fully mixed with standard pedotheque, obtain being equipped with the diffusion boat sample 1 for the treatment of soil alkaline hydrolysis, 2., repeating step 1. 4 times, obtain being equipped with treat soil alkaline hydrolysis diffusion boat sample 2, be equipped with treat soil alkaline hydrolysis diffusion boat sample 3, be equipped with and treat the diffusion boat sample 4 of soil alkaline hydrolysis and the diffusion boat sample 5 for the treatment of soil alkaline hydrolysis is housed,
Two, alkaline hydrolysis: by be equipped with treat soil alkaline hydrolysis diffusion boat sample 1, be equipped with treat soil alkaline hydrolysis diffusion boat sample 2, be equipped with treat soil alkaline hydrolysis diffusion boat sample 3, be equipped with and treat the diffusion boat sample 4 of soil alkaline hydrolysis and the diffusion boat sample 5 for the treatment of soil alkaline hydrolysis is housed that to put into temperature be the constant temperature oven of 40 ℃, alkaline hydrolysis diffusion 24h; Take off the frosted glass on diffusion boat again, use the hair side on distilled water flushing frosted glass, washing fluid joins in the interior chamber of diffusion boat; Re-use sulfuric acid standard solution respectively 5 of titration treat in the diffusion boat sample of soil alkaline hydrolysis the ammonia in mixed liquor in chamber; It is titration end-point that mixed liquor in diffusion boat in chamber becomes blush from blue look, stops titration and records the volume V that consumes sulfuric acid standard solution in titration 1, V 2, V 3, V 4and V 5;
Three, prepare blank sample: get a clean diffusion boat, to the interior chamber of diffusion boat, adding 2mL boric acid concentration is boric acid-mixed indicator of 20g/L, then at diffusion boat mistress edge, coat Arabic gum, cover frosted glass, rotation, make diffusion boat and frosted glass completely bonding, turn-off frosted glass on one side again, make diffusion boat mistress expose a slit, the sodium hydroxide solution that is 1mol/L by 10mL concentration again joins in diffusion boat mistress by slit, make again frosted glass cover tightly diffusion boat, re-use bungee circle tight, frosted glass is fixed on diffusion boat, again diffusion boat is put into temperature and is the constant temperature oven of 40 ℃, alkaline hydrolysis diffusion 24h, take off the frosted glass on diffusion boat again, use the hair side on distilled water flushing frosted glass, washing fluid joins in the interior chamber of diffusion boat, re-use the ammonia in mixed liquor in the interior chamber of sulfuric acid standard solution titration diffusion boat sample, it is titration end-point that mixed liquor in diffusion boat in chamber becomes blush from blue look, stops titration and records the volume V that consumes sulfuric acid standard solution in titration 0,
Four, utilize following computing formula to calculate respectively soil alkali-hydrolyzable nitrogen content W in 5 samples 1, W 2, W 3, W 4and W 5;
W i = C × ( V - V 0 ) × 14 m × 1000 ;
In above-mentioned formula:
W i---soil alkali-hydrolyzable nitrogen content in sample, unit is (mg/kg);
C---the concentration of sulfuric acid standard solution, unit is mole every liter (mol/L);
V---in titration, consume the volume of sulfuric acid standard solution, unit is milliliter (mL);
V 0---the volume of titration empty control sample consumption consumption sulfuric acid standard solution, unit is milliliter (mL);
14---the molal weight of nitrogen-atoms, unit is for gram often rubbing (g/mol);
M---the quality of wind desiceted soil earth sample in sample, unit is (g);
Five, W step 4 being obtained 1, W 2, W 3, W 4and W 5average and obtain soil alkali-hydrolyzable nitrogen content.
Boric acid concentration described in contrast test step 2 is boric acid-mixed indicator of 20g/L;
The concentration of the sulfuric acid standard solution of contrast test step 2 described in is 3. 0.01mol/L;
The concentration of the sulfuric acid standard solution described in contrast test step 3 is 0.01mol/L.
The W that contrast test is calculated 1, W 2, W 3, W 4, W 5and mean value lists in table 2, table 2 is the testing result of test one;
Table 2
Sample number 1 2 3 4 5 Mean value Standard value Relative standard deviation
Measured value mg/kg 61 69 63 68 62 64.6 66±8 5.65％
As known from Table 2, mean value is 64.6mg/kg, and soil alkali-hydrolyzable nitrogen content is 64.6mg/kg; The standard value of test is 66 ± 8, and relative standard deviation is 5.65%.
The relative standard deviation of test one is 4.52%, and the relative standard deviation of contrast test is 5.65%, and the relative standard deviation of test one is lower than the relative standard deviation of contrast test.

Claims (4)

1. an assay method for soil alkali-hydrolyzable nitrogen content, is characterized in that a kind of assay method of soil alkali-hydrolyzable nitrogen content specifically completes according to the following steps:
One, the plastics diffusion boat sample for the treatment of soil alkaline hydrolysis is equipped with in preparation: 1., first take air-dry pedotheque and 0.2g ferrous sulphate powder that 2.00g crosses 2mm aperture sieve; Then air-dry pedotheque and the 0.2g ferrous sulphate powder of the 2.00g taking being crossed to 2mm aperture sieve mix, then are laid in the mistress of plastics diffusion boat; To the interior chamber of plastics diffusion boat, adding 2mL boric acid concentration is boric acid-mixed indicator of 20g/L, re-using silica gel bowl cover covers above plastics diffusion boat, opening silica gel bowl cover makes plastics diffusion boat mistress expose a slit, the sodium hydroxide solution that is 1mol/L by 10mL concentration again joins in plastics diffusion boat mistress by slit, then covers silica gel bowl cover; Obtain being equipped with the plastics diffusion boat sample 1 for the treatment of soil alkaline hydrolysis; 2., repeating step one 1. 4 times, obtain being equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 2, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 3, be equipped with and treat the plastics diffusion boat sample 4 of soil alkaline hydrolysis and the plastics diffusion boat sample 5 for the treatment of soil alkaline hydrolysis is housed;
Two, alkaline hydrolysis: 1., by treat soil alkaline hydrolysis plastics diffusion boat sample 1, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 2, be equipped with treat soil alkaline hydrolysis plastics diffusion boat sample 3, be equipped with and treat the plastics diffusion boat sample 4 of soil alkaline hydrolysis and be equipped with to treat that the plastics diffusion boat sample 5 of soil alkaline hydrolysis packs in diffusion boat supporting plate, then diffusion boat supporting plate is placed on culturing rack;
2., culturing rack is put into temperature is the constant temperature oven of 40 ℃, alkaline hydrolysis diffusion 24h; Re-use sulfuric acid standard solution respectively 5 of titration treat the ammonia in boric acid-mixed indicator that in the plastics diffusion boat sample of soil alkaline hydrolysis, chamber mesoboric acid concentration is 20g/L; When the boric acid concentration boric acid-mixed indicator that is 20g/L becomes blush from blue look, be titration end-point, stop titration and record the volume V that consumes sulfuric acid standard solution in titration 1, V 2, V 3, V 4and V 5;
Three, prepare blank sample: get a clean plastics diffusion boat, to the interior chamber of plastics diffusion boat, adding 2mL boric acid concentration is again boric acid-mixed indicator of 20g/L, re-using silica gel bowl cover covers above plastics diffusion boat, opening silica gel bowl cover makes plastics diffusion boat mistress expose a slit, the sodium hydroxide solution that is 1mol/L by 10mL concentration again joins in plastics diffusion boat mistress by slit, then covers silica gel bowl cover; Again plastics diffusion boat is put into temperature and is the constant temperature oven of 40 ℃, alkaline hydrolysis diffusion 24h; Re-use the ammonia in boric acid-mixed indicator that in sulfuric acid standard solution titration plastics diffusion boat, chamber boric acid concentration is 20g/L; When the boric acid concentration boric acid-mixed indicator that is 20g/L becomes blush from blue look, be titration end-point, stop the volume V that titration record consume sulfuric acid standard solution 0;
Four, utilize following computing formula to calculate respectively soil alkali-hydrolyzable nitrogen content W in 5 samples 1, W 2, W 3, W 4and W 5;
W i = C × ( V - V 0 ) × 14 m × 1000 ;
In above-mentioned formula:
W i---soil alkali-hydrolyzable nitrogen content in sample, unit is (mg/kg);
C---the concentration of sulfuric acid standard solution, unit is mole every liter (mol/L);
V---in titration, consume the volume of sulfuric acid standard solution, unit is milliliter (mL);
V 0---titration empty control sample consumes the volume of sulfuric acid standard solution, and unit is milliliter (mL);
14---the molal weight of nitrogen-atoms, unit is for gram often rubbing (g/mol);
M---the quality of wind desiceted soil earth sample in sample, unit is (g);
Five, W step 4 being obtained 1, W 2, W 3, W 4and W 5average and obtain soil alkali-hydrolyzable nitrogen content.
2. the assay method of a kind of soil alkali-hydrolyzable nitrogen content according to claim 1, the compound method that it is characterized in that boric acid-mixed indicator that the boric acid concentration described in step 1 is 1. 20g/L is: 20g boric acid is dissolved in the hot distilled water of 950mL, naturally after cooling, the mixed indicator that adds 20mL, after fully mixing, the sodium hydroxide solution that to drip concentration be 0.1mol/L, to the pH value of boric acid-mixed indicator be 4.5, then boric acid-mixed indicator is diluted to 1L; Wherein, described mixed indicator be the bromcresol green of 0.09g and 0.066g methyl red to be dissolved in 100mL massfraction be in 95% ethanolic solution, formulated.
3. the assay method of a kind of soil alkali-hydrolyzable nitrogen content according to claim 1, the concentration that it is characterized in that the sulfuric acid standard solution described in step 2 is 2. 0.01mol/L.
4. the assay method of a kind of soil alkali-hydrolyzable nitrogen content according to claim 1, the concentration that it is characterized in that the sulfuric acid standard solution described in step 3 is 0.01mol/L.
CN201410317310.6A 2014-07-04 2014-07-04 Method for detecting content of soil alkali-hydrolyzable nitrogen Pending CN104111256A (en)

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Cited By (7)

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CN104991036A (en) * 2015-08-03 2015-10-21 河南广电计量检测有限公司 Determination method for soil alkali-hydrolyzable nitrogen
CN109470836A (en) * 2018-10-23 2019-03-15 中国科学院合肥物质科学研究院 A kind of Soil K+adsorption alkali-hydrolyzable nitrogen batch detection device
CN109470835A (en) * 2018-10-23 2019-03-15 中国科学院合肥物质科学研究院 A kind of Soil K+adsorption alkali-hydrolyzable nitrogen processing unit
CN109490509A (en) * 2018-10-23 2019-03-19 中国科学院合肥物质科学研究院 A kind of soil alkali-hydrolyzable nitrogen content measuring laying device
CN109490508A (en) * 2018-10-23 2019-03-19 中国科学院合肥物质科学研究院 A kind of nitrogenous test expectation system of soil alkali-hydrolyzable nitrogen
CN109900694A (en) * 2019-01-18 2019-06-18 河南省烟草公司平顶山市公司 A kind of measuring method of vega soil alkaline hydrolysis nitrogen content
CN112505292A (en) * 2020-11-17 2021-03-16 中国环境科学研究院 Culture device and culture method for nitrogen hydrolysis experiment

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104991036A (en) * 2015-08-03 2015-10-21 河南广电计量检测有限公司 Determination method for soil alkali-hydrolyzable nitrogen
CN109470836A (en) * 2018-10-23 2019-03-15 中国科学院合肥物质科学研究院 A kind of Soil K+adsorption alkali-hydrolyzable nitrogen batch detection device
CN109470835A (en) * 2018-10-23 2019-03-15 中国科学院合肥物质科学研究院 A kind of Soil K+adsorption alkali-hydrolyzable nitrogen processing unit
CN109490509A (en) * 2018-10-23 2019-03-19 中国科学院合肥物质科学研究院 A kind of soil alkali-hydrolyzable nitrogen content measuring laying device
CN109490508A (en) * 2018-10-23 2019-03-19 中国科学院合肥物质科学研究院 A kind of nitrogenous test expectation system of soil alkali-hydrolyzable nitrogen
CN109490508B (en) * 2018-10-23 2021-10-29 中国科学院合肥物质科学研究院 Soil alkaline hydrolysis nitrogen-containing test prediction system
CN109900694A (en) * 2019-01-18 2019-06-18 河南省烟草公司平顶山市公司 A kind of measuring method of vega soil alkaline hydrolysis nitrogen content
CN112505292A (en) * 2020-11-17 2021-03-16 中国环境科学研究院 Culture device and culture method for nitrogen hydrolysis experiment
CN112505292B (en) * 2020-11-17 2022-02-18 中国环境科学研究院 Culture device and culture method for nitrogen hydrolysis experiment

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Application publication date: 20141022