CN103364540A - Technology for detecting urine G-6-PD activity and supplementary reagents - Google Patents
Technology for detecting urine G-6-PD activity and supplementary reagents Download PDFInfo
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- CN103364540A CN103364540A CN 201210093836 CN201210093836A CN103364540A CN 103364540 A CN103364540 A CN 103364540A CN 201210093836 CN201210093836 CN 201210093836 CN 201210093836 A CN201210093836 A CN 201210093836A CN 103364540 A CN103364540 A CN 103364540A
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Abstract
The invention relates to a technology for detecting G-6-PD activity and supplementary chemical reagents, instruments and automatic biochemical analyzer reagents, and is applicable to detecting the urine G-6-PD activity. The conventional technology for detecting the G-6-PD activity has the following defects: the gene detection technology of extracting DNA is high in cost and complex in operation and requires high quality of operators; the hemolysis cell blood biochemical analyzer detection technology is complex in operation and insufficient in specificity; and the hemolysis paper-strip-method detection technology is complex in operation and poor in specificity. In order to solve the problems, the invention provides the technology for detecting the G-6-PD activity and supplementary chemical reagents, instruments and automatic biochemical analyzer reagents. The detection technology is convenient, practical, simple and easy to operate, good in specificity, high in sensitivity, low in cost and low in quality requirement for operators.
Description
Technical field
The present invention relates to a kind of technology of novel detection G-6-PD activity and supporting dry chemistry reagent and instrument, automatic biochemical analyzer reagent, be applicable to detect urine G-6-PD active.
Background technology
There is following shortcoming in the technology that detects at present the G-6-PD activity: extracting DNA carries out that the technique of gene detection cost is high, complicated operation, require operator quality high; Haemolysis ball blood biochemical instrument detection technique complicated operation, specificity deficiency; Haemolysis ball paper disk method detection technique complex operation, poor specificity.The human body G-6-PD overwhelming majority is present in the red blood cell, and old and feeble red blood cell mainly destroys at spleen, and G6PD is flowed out by urine through kidney with blood flow, thereby can be active by urine detection G-6-PD.
Summary of the invention
In order to overcome the above problems, the invention provides a kind of detection technique of novel detection G-6-PD activity and supporting dry chemistry reagent and instrument, automatic biochemical reagent.This detection technique is convenient and practical, operation is simple and easy, specificity good, susceptibility is high, cost is low, operating personnel are not high to competency profiling.
The technical solution adopted for the present invention to solve the technical problems is: 1. " wong's method " detects technology and the matched reagent of urine G-6-PD activity, it is characterized in that: utilize urine specimen to detect G-6-PD.2. " wong's method " detects technology and the matched reagent of urine G-6-PD activity, and it is characterized in that: robotization reagent R1 is by MgCL
2, the composition such as NADP, antiseptic, stabilizing agent, damping fluid, reagent R2 is comprised of damping fluid, G6P, antiseptic, stabilizing agent etc.3. " wong's method " detects technology and the matched reagent of urine G-6-PD activity, and it is characterized in that: dry chemistry reagent is made the 3rd layer of intrinsic reagent water accepting layer that dry powder is inserted drying chemical reagent paper in proportion by 6-phosphogluconic acid solution, G6P solution, nitro blue tetrazolium, phenazine methosulfate, damping fluid etc.
The invention has the beneficial effects as follows: this detection technique is convenient and practical, operation is simple and easy, specificity good, susceptibility is high, cost is low, operating personnel are not high to competency profiling.
Specific embodiments
Dry chemical urine G-6-PD activity detection: leave and take inspection person's urine, pour test tube into, put into dry chemical scrip, put into the dry chemical instrument after leaving standstill, owing to change color appears in the reagent reacting of G-6-PD in the urine and drying chemical reagent paper, the dry chemical instrument is by Optical Absorption and reflection reading result.Dry chemistry reagent is made the 3rd layer of intrinsic reagent water accepting layer that dry powder is inserted drying chemical reagent paper in proportion by 6-phosphogluconic acid solution, G6P solution, nitro blue tetrazolium, phenazine methosulfate, damping fluid etc.
Automatic biochemical analyzer urine G6PD organized enzyme detection method: leave and take inspection person's urine, pour machine testing on the test tube automatic biochemical analyzer into.Robotization reagent R1 is by MgCL
2, the composition such as NADP, antiseptic, stabilizing agent, damping fluid, reagent R2 is comprised of damping fluid, G6P, antiseptic, stabilizing agent etc.Rate method is measured, and the detection wavelength is 340nm.
Claims (3)
1. technology and a matched reagent that detects urine G-6-PD activity is characterized in that: utilize urine specimen to detect G-6-PD.
2. technology and matched reagent that detects urine G-6-PD activity, it is characterized in that: robotization reagent R1 is by MgCL
2, the composition such as NADP, antiseptic, stabilizing agent, damping fluid, reagent R2 is comprised of damping fluid, G6P, antiseptic, stabilizing agent etc.
3. technology and matched reagent that detects urine G-6-PD activity, it is characterized in that: dry chemistry reagent is made the 3rd layer of intrinsic reagent water accepting layer that dry powder is inserted drying chemical reagent paper in proportion by 6-phosphogluconic acid solution, G6P solution, nitro blue tetrazolium, phenazine methosulfate, damping fluid etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201210093836 CN103364540A (en) | 2012-03-27 | 2012-03-27 | Technology for detecting urine G-6-PD activity and supplementary reagents |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210093836 CN103364540A (en) | 2012-03-27 | 2012-03-27 | Technology for detecting urine G-6-PD activity and supplementary reagents |
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| CN103364540A true CN103364540A (en) | 2013-10-23 |
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| CN 201210093836 Pending CN103364540A (en) | 2012-03-27 | 2012-03-27 | Technology for detecting urine G-6-PD activity and supplementary reagents |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109609594A (en) * | 2018-12-24 | 2019-04-12 | 苏州科铭生物技术有限公司 | A kind of glucose 6-phosphate assay kit and method based on micromethod |
| CN111060503A (en) * | 2020-01-02 | 2020-04-24 | 青岛汉唐生物科技有限公司 | G-6-PD detection kit free from anemia and detection method thereof |
| CN111638212A (en) * | 2020-06-15 | 2020-09-08 | 江南大学 | Method for detecting content of glucose-6-phosphate based on nano enzyme |
| CN115078341A (en) * | 2022-08-22 | 2022-09-20 | 上海执诚生物科技有限公司 | Reagent for detecting glucose-6-phosphate dehydrogenase and application thereof |
-
2012
- 2012-03-27 CN CN 201210093836 patent/CN103364540A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109609594A (en) * | 2018-12-24 | 2019-04-12 | 苏州科铭生物技术有限公司 | A kind of glucose 6-phosphate assay kit and method based on micromethod |
| CN111060503A (en) * | 2020-01-02 | 2020-04-24 | 青岛汉唐生物科技有限公司 | G-6-PD detection kit free from anemia and detection method thereof |
| CN111638212A (en) * | 2020-06-15 | 2020-09-08 | 江南大学 | Method for detecting content of glucose-6-phosphate based on nano enzyme |
| CN111638212B (en) * | 2020-06-15 | 2021-07-27 | 江南大学 | Method for detecting content of glucose-6-phosphate based on nano enzyme |
| CN115078341A (en) * | 2022-08-22 | 2022-09-20 | 上海执诚生物科技有限公司 | Reagent for detecting glucose-6-phosphate dehydrogenase and application thereof |
| CN115078341B (en) * | 2022-08-22 | 2022-11-29 | 上海执诚生物科技有限公司 | Reagent for detecting glucose-6-phosphate dehydrogenase and application thereof |
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Application publication date: 20131023 |