CN104094784A - Cultivation method for improving quality of astragalus - Google Patents

Cultivation method for improving quality of astragalus Download PDF

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Publication number
CN104094784A
CN104094784A CN201410346451.0A CN201410346451A CN104094784A CN 104094784 A CN104094784 A CN 104094784A CN 201410346451 A CN201410346451 A CN 201410346451A CN 104094784 A CN104094784 A CN 104094784A
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astragalus
radix astragali
cultivation method
blue
quality
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CN201410346451.0A
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梁建萍
周然
冯前进
李忠星
刘惠娟
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Shanxi Agricultural University
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Shanxi Agricultural University
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Abstract

The invention discloses a cultivation method for improving the quality of astragalus. The cultivation method includes performing light quality processing on 3-6 perennial astragalus on the basis of semi-wild cultivation management for conventional artificial seed sowing assisted reproduction and growth; constructing film supports in astragalus fields when the heights of growing plants reach 30cm from the last third of May to the beginning of June in a growing period; covering the astragalus by blue films for a growing season until leaves of the astragalus fall; conventionally managing the fields until the astragalus is harvested. The wavelength of blue light of the blue films ranges from 400nm to 450nm, the wave crest of the blue light is 420nm, and the light transmittance of the blue films is 85%. The cultivation method has the advantages that the cultivation method is simple, and the investment cost is low; the accumulation of flavone in roots of the astragalus is increased by 15% after the blue films cover the astragalus and the astragalus is processed as compared with a natural light condition on the premise that the dry weights of the roots of the astragalus are not obviously changed, the accumulation of saponin in the roots of the astragalus is increased by 6.5%, accordingly, the quality of the astragalus is obviously improved, and an input-output ratio of the cultivation method reaches 4.5 times at least.

Description

Improve the cultivation method of Radix Astragali quality
Technical field
The present invention relates to Radix Astragali culture technique field, particularly by changing light quality, improve the cultivation method of Radix Astragali quality.
Background technology
The Radix Astragali, has another name called continuous stilbene, herbaceos perennial, main product in Inner Mongol of China, the ground such as Shanxi, Gansu, Heilungkiang.The Radix Astragali is medicinal and edible plants, its medicinal history in existing more than 2000 year so far.The Radix Astragali is warm in nature, and taste is sweet, and tepor is returned lung, spleen, liver, kidney channel.The Radix Astragali has the effect of admittedly de-, the de-sore myogenic of invigorating qi for consolidating superficies, arrest sweating, inducing diuresis to reduce edema.Be used for the treatment of the deficiency of vital energy weak, the sinking of qi of middle-jiao, rushes down prolapse of the anus for a long time, the uterine bleeding of having blood in stool, and exterior deficiency spontaneous perspiration, ulcer is difficult bursts, and bursts and does not hold back for a long time, and the deficiency of blood is sallow, and interior heat is quenched one's thirst, chronic nephritis, albuminuria, diabetes etc.The bowl spares of astragalus root benefit gas, raw by the de-sore of solid table.The Radix Astragali is also often edible pure natural product of the common people, and folklore the saying of " often drinking astragali seu Hedysari Decoction, disease-prevention health health ", through the conventional Radix Astragali with the precious glossy ganoderma of fine jade decoct soup or soaked generation tea-drinking, there is good disease-prevention health effect.
Modern medicine study shows, the main pharmacodynamics composition in astragalus root has three classes, be respectively Radix Astragali flavone class material, Astrageloside material and astragalus polyose class material, and these active components is also the secondary metabolite of the Radix Astragali.Research shows, in plant corpus, Secondary Metabolite Contents is subject to the impact of environmental change, and the environmental conditions such as arid, high temperature, ultraviolet ray can be induced the synthetic of secondary metabolites and be accumulated, and then the drug effect of raising medicinal plant.At present, the research of domestic relevant Radix Astragali effective component concentrates on kind, metabolic pathway, chemical property, pharmacological effect and the distribution in plant corpus thereof etc. mostly, and environmental factor is less to the dynamic accumulation research of secondary metabolite in body, especially the accumulation dynamic report of illumination regulation and control Radix Astragali its secondary metabolites content is very few.Therefore, accumulation and the dynamic change of research Radix Astragali content of drug effect components under different light medium condition have important practical significance.Study its vegetation growth of plant and effective component accumulation dynamic and change, from the synthetic ecological mechanism of Physiology and biochemistry Study on Level effective component, for improving Radix Astragali output in producing and quality, the effective Radix Astragali cultivation step of foundation provide technological guidance.
The wild Radix Astragali, owing to digging excessively coyoting, causes quantity sharply to reduce in recent years, has at any time the danger of extinction.For preventing the extinction of its species, country has been classified as three-level protective plant, and restriction is adopted plane and excavated seedling, takes the circumstances into consideration to gather after seed maturity, advocates and greatly develops artificial planting simultaneously, enters at present the medicinal Radix Astragali and is mainly derived from artificial cultivation.And the quality of medicinal material existing in the current artificial cultivation Radix Astragali is unstable and quality of medicinal material decline problem is the bottleneck of puzzlement Radix Astragali cultivation development.The polysaccharide, saponin, the flavones isoreactivity component content that by culture technique, improve in the Radix Astragali become one of important content of Radix Astragali research.
Summary of the invention
The invention provides a kind of cultivation method that improves Radix Astragali quality, by changing the light quality condition in Radix Astragali process of growth, promote the dynamic accumulation of Radix Astragali main active, promote Milkvetch Root quality, the present invention is that investment is little, simple to operate, the significant cultivation method of raising Radix Astragali quality effect.
The present invention improves the cultivation method of Radix Astragali quality, comprises the steps:
(1) the semi-wild cultivation management of conventional manually sow seeds aid propagation and growth;
(2) the raw Radix Astragali of 3-6 is carried out to light quality processing, at the beginning of late May to 6 month, when plant is during up to 30cm, the Radix Astragali build film frame, with blue film, cover the Radix Astragali, until fall leaves;
(3) conventional field management is to results.
The blue light wavelength of described blue film is 400~450nm, and crest is 420nm, and light transmittance is 85%, and by Shanghai, Wei Kang coloured plastics material factory produces.
The present invention improves the cultivation method of Radix Astragali quality and the comparative trial contrasting
1. test method
Test is carried out in beautiful pearl Radix Astragali base, Hunyuan County, Datong City, Shanxi Province, is positioned at mountain range, Hengshan Mountain, belongs to the continental monsoon climate of temperate zone arid and semi-arid, 6.2 ℃ of average temperatures of the whole year, average-12 ℃ of January, average 21.6 ℃ of July.Average annual rainfall 429.4mm, annual sunshine amount 2696.3h.Geographical coordinate is north latitude 39 .21’~39 53 ', east longitude 113 .22’~113 58 ', height above sea level is 1300~1800m approximately, 20 °~50 ° of the gradients, and soil mostly is sandy yellow earth, and soft permeability is better.Test adopts semi-wild astragalus mongolicus, and the semi-wild cultivation management of manually sow seeds aid propagation and growth started examine on the spot, in adjacent plot, carries out sign in mid-May, 2013, at the beginning of late May to 6 month, starts overlay film when the average plant height of the Radix Astragali reaches 30cm.If two processing of blue film and natural daylight, each is processed and three samples is set respectively (repetition), the long 5m in sample ground, wide 2m, every sample ground 400 strain Radix Astragali plant, spacing in the rows 15cm, line-spacing 20cm.The selected Radix Astragali age is respectively life in 3 years, 5 years, 6 years.Growing period test index comprises: chlorophyll content in leaf blades, superoxide dismutase (SOD), peroxidase (POD), phenylalnine ammonialyase (PAL enzyme); After results, test index comprises: astragalus root dry matter weight and flavones, saponin(e, polyoses content, flavones individual plant economic flow rate, saponin(e individual plant economic flow rate and polysaccharide individual plant economic flow rate in astragalus root.
2. result of the test
Cultivation method of the present invention with the result of the test that contrasts cultivation method comparison in Table 1.
Table 1 cultivation method of the present invention and the result of the test that contrasts cultivation method comparison
Note: ※ ※represent that statistical analysis difference is extremely remarkable; represent statistical analysis significant difference.
From table 1, adopt cultivation method of the present invention to contrast cultivation method comparison with natural daylight, the former is significantly higher than the latter by the Radix Astragali blade Determination of Chlorophyll content utmost point; In the former astragalus root, the activity of SOD, POD, tri-kinds of enzymes of PAL is significantly higher than the latter; In the former root flavones and flavones individual plant economic flow rate, saponin(e and saponin(e individual plant economic flow rate significantly or the utmost point be significantly higher than the latter; In the former astragalus root dry weight, root, polysaccharide and polysaccharide individual plant economic flow rate be lower than the latter, but difference is not remarkable.
Result shows, (1) after blue film covers and processes, chlorophyll whole vegetative period average content high compared with natural daylight, illustrate that blue light has promoted that the Radix Astragali is chlorophyllous and synthesized; (2) after blue film covers processing, in astragalus root, flavones and saponin(e accumulation and their individual plant economic flow rate thereof increase significantly compared with natural daylight, and in astragalus root dry weight, root, polysaccharide and polysaccharide individual plant economic flow rate slightly decline compared with natural daylight, but difference is not remarkable; (3) after blue film covers processing, the Radix Astragali synthesizes relevant enzyme to secondary metabolites in vegetative period, and the average activity of SOD, POD, tri-kinds of enzymes of PAL significantly improves compared with natural daylight.It is the highest that SOD, POD and PAL are all fructescence activity, and flavones, saponin content reach maximum at fructescence too, show thus, SOD, POD, tri-kinds of enzymes of PAL can regulate the Radix Astragali to adapt to blue light light quality environment under blue light, promote the dynamic accumulation of flavonoids and saponins.Although the accumulation of astragalus polyose declines to some extent under blue light, compares difference not remarkable with natural daylight.
beneficial effect of the present invention
The present invention regulates and controls to improve Radix Astragali quality by applying blue light, and method is simple, and cost of investment is little, and after blue film covers processing, in astragalus root, accumulation of flavonoids amount is compared and improved 15% with natural daylight, and saponin(e accumulation has improved 6.5%, has significantly improved the quality of the Radix Astragali.
embodiment 1
In beautiful pearl Radix Astragali base, Hunyuan County, Datong City, Shanxi Province, the 3 years raw astragalus mongolicus of take are examination material, on June 1st, 2013, during as the average plant height 30cm of the Radix Astragali, build film frame, and the blue film of producing with Shanghai Wei Kang coloured plastics material factory covers the Radix Astragali, until fallen leaves are gathered in the crops.Measure flavones content, saponin content and polyoses content in astragalus root dry weight, root, compare with the Radix Astragali that does not cover the adjacent plot of blue film, covering blue film increases by 0.48% than the unit are astragalus root dry weight not covering, and in root, flavones content has improved 13.9%, and in root, saponin content has improved 8.1%.Because Radix Astragali quality improves the economic benefit of bringing, be to cover 4.5 times that blue film cost drops into.
embodiment 2
In beautiful pearl Radix Astragali base, Hunyuan County, Datong City, Shanxi Province, the 5 years raw astragalus mongolicus of take are examination material, on May 28th, 2013, during as the average plant height 30cm of the Radix Astragali, build film frame, and the blue film of producing with Shanghai Wei Kang coloured plastics material factory covers the Radix Astragali, until fallen leaves are gathered in the crops.Measure flavones content, saponin content and polyoses content in astragalus root dry weight, root, compare with the Radix Astragali that does not cover the adjacent plot of blue film, cover blue film and reduced by 0.21% than the unit are astragalus root dry weight not covering, in root, flavones content has improved 16.9%, and in root, saponin content has improved 7.1%.Because Radix Astragali quality improves the economic benefit of bringing, be to cover 5.1 times that blue film cost drops into.
embodiment 3
In beautiful pearl Radix Astragali base, Hunyuan County, Datong City, Shanxi Province, the 6 years raw astragalus mongolicus of take are examination material, on June 3rd, 2013, during as the average plant height 30cm of the Radix Astragali, build film frame, and the blue film of producing with Shanghai Wei Kang coloured plastics material factory covers the Radix Astragali, until fallen leaves are gathered in the crops.Measure flavones content, saponin content and polyoses content in astragalus root dry weight, root, compare with the Radix Astragali that does not cover the adjacent plot of blue film, cover blue film and reduced by 0.16% than the unit are astragalus root dry weight not covering, in root, flavones content has improved 15.3%, and in root, saponin content has improved 6.2%.Because Radix Astragali quality improves the economic benefit of bringing, be to cover 4.8 times that blue film cost drops into.

Claims (2)

1. a cultivation method that improves Radix Astragali quality, comprises the steps:
(1) the semi-wild cultivation management of conventional manually sow seeds aid propagation and growth;
(2) the raw Radix Astragali of 3-6 is carried out to blue light processing vegetative period, at the beginning of late May to 6 month, when plant is during on average up to 30cm, the Radix Astragali build film frame, with blue film, cover the Radix Astragali, until fall leaves;
(3) conventional field management is to results.
2. the cultivation method of raising Radix Astragali quality according to claim 1, is characterized in that, the blue light wavelength of described blue film is 400~450nm, and crest is 420nm, and light transmittance is 85%.
CN201410346451.0A 2014-07-21 2014-07-21 Cultivation method for improving quality of astragalus Pending CN104094784A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105145091A (en) * 2015-09-21 2015-12-16 甘肃省科学院生物研究所 Greenhouse accelerated culture method for colored zantedeschia in northwest region
CN105533402A (en) * 2015-12-23 2016-05-04 上海应用技术学院 Method for increasing contents of triterpenes and flavonoid substances in postharvest tomato fruits during storage and transportation processes
CN105837326A (en) * 2016-03-31 2016-08-10 平顶山市绿禾农业科技开发有限公司 Method for promoting root growth of Radix Astragali
CN111133937A (en) * 2018-11-05 2020-05-12 浙江省农业科学院 Cultivation method for improving flavone and polyphenol content of waxberry fruits by using colored greenhouse film
CN111587785A (en) * 2020-05-06 2020-08-28 内蒙古自治区生物技术研究院 Method for culturing astragalus membranaceus hairy roots for promoting accumulation of flavonoids

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CN102835236A (en) * 2012-08-28 2012-12-26 刘俊希 Semi-wild astragalus mongholicus sowing method
CN103430718A (en) * 2013-07-24 2013-12-11 王运海 Planting method of astragalus membranaceus
CN103688696A (en) * 2012-09-28 2014-04-02 深圳华大基因科技有限公司 Planting method for root and stem type traditional Chinese medicinal materials

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JPH0638635A (en) * 1991-12-05 1994-02-15 Nippon Soda Co Ltd Wave length changing material
CN102550244A (en) * 2011-12-21 2012-07-11 内蒙古广一药材种植有限责任公司 Planting method capable of improving astragalus mongholicus yield
CN102835236A (en) * 2012-08-28 2012-12-26 刘俊希 Semi-wild astragalus mongholicus sowing method
CN103688696A (en) * 2012-09-28 2014-04-02 深圳华大基因科技有限公司 Planting method for root and stem type traditional Chinese medicinal materials
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105145091A (en) * 2015-09-21 2015-12-16 甘肃省科学院生物研究所 Greenhouse accelerated culture method for colored zantedeschia in northwest region
CN105533402A (en) * 2015-12-23 2016-05-04 上海应用技术学院 Method for increasing contents of triterpenes and flavonoid substances in postharvest tomato fruits during storage and transportation processes
CN105837326A (en) * 2016-03-31 2016-08-10 平顶山市绿禾农业科技开发有限公司 Method for promoting root growth of Radix Astragali
CN111133937A (en) * 2018-11-05 2020-05-12 浙江省农业科学院 Cultivation method for improving flavone and polyphenol content of waxberry fruits by using colored greenhouse film
CN111133937B (en) * 2018-11-05 2022-03-25 浙江省农业科学院 Cultivation method for improving flavone and polyphenol content of waxberry fruits by using colored greenhouse film
CN111587785A (en) * 2020-05-06 2020-08-28 内蒙古自治区生物技术研究院 Method for culturing astragalus membranaceus hairy roots for promoting accumulation of flavonoids

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Application publication date: 20141015