CN104082037A - Method for postprocessing of hericium erinaceus fermentation mycelium solution and preparing mycelium powder through biological enzyme - Google Patents
Method for postprocessing of hericium erinaceus fermentation mycelium solution and preparing mycelium powder through biological enzyme Download PDFInfo
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Abstract
The invention provides a method for postprocessing of hericium erinaceus fermentation mycelium solution and preparing mycelium powder through biological enzyme. The method solves the problems that according to existing hericium erinaceus mycelium, the fermentation period is long, contamination is prone to appearing, and the final product quality is not stable and also solves the problems that the number of mycelium fibers in an existing hericium erinaceus fermentation mycelium solution is large, the fibers cannot be easily separated, and further preparation of the mycelium powder is limited. The method comprises the steps of preparing a culture medium, activating a culture, inoculating, performing cultivating, expanding fermentation, performing enzymolysis, performing concentrating, performing homogenizing, performing spraying and performing drying. The method for preparing the culture medium comprises the steps of preparing an inclined plane culture medium, preparing a shake flask culture medium, preparing a seeding tank culture medium, and preparing a fermentation tank culture medium. The method is widely applied to processing the hericium erinaceus mycelium powder in the food industry.
Description
Technical field
The present invention relates to a kind of preparation method of healthy food, be specifically related to the method that biology enzyme is assisted post processing Hericium erinaceus fermented hypha liquid and prepared hypha powder.
Background technology
Hericium erinaceus (
hericium erinaceus) claim again Hericium erinaceus; drug efficacy study shows; it contains the macromolecular compounds such as polypeptide, polysaccharide and acid amides; there is the body's hypoxia tolerance of raising, raising painstaking effort output quantity, accelerate blood circulation, reduction blood sugar and blood pressure, protection liver; and can be by strengthening the phagocytosis of peritoneal macrophage and then strengthening the immunologic function of body and improve immunity, it also has good protective effect to stomach lining in addition.But in food industry, fruit body or mycelium obtain by solid culture, its incubation time needs certain cycle, and the time of obtaining fruit body needs longer, thereby makes its popularization have certain limitation.And modern study is found, effect material that Hericium erinaceus nutrient hypha and fruit body contain similar amount.Submerged fermentation can obtain a large amount of mycelium, and the cycle short, be convenient to industrialized production and automation control, also can be made into through serial post processing the active mycelium powder of stay in grade, be convenient to the edible and marketing of people.
Hericium mycelium powder is that the submerged fermentation of high-quality hedgehog fungus bacterial is cultivated to the hericium mycelium liquid obtaining, the active hericium mycelium xeraphium obtaining after cellulase degradation processing, concentrated, homogeneous and spraying are dry.Its technical process is: slant strains → one-level shake-flask seed → secondary seed tank bacterial classification → fermentation tank expands be dried → hericium mycelium dry powder → finished product of concentrate → concentrate homogeneous → Highspeedcentrifugingandsprayingdrier of cultivation → cellulase degradation processing → external circulation evaporator.
Summary of the invention
The present invention in order to solve long, the easily microbiological contamination of existing hericium mycelium solid fermentation cycle, final products quality is unstable, and in existing liquid fermentation hericium mycelium liquid, is difficult for further separating and preparing the problems such as hypha powder because mycelia fiber.And adopt the hericium mycelium liquid of a kind of biology enzyme enzymolysis after submerged fermentation, and after concentrated, homogeneous, spraying are dry, form a kind of become the instant powder that reconstitutes of hericium mycelium of instant, comprehensive nutrition, its method comprises the following steps:
A: the preparation of slant medium
According to mass percent potato 18 ~ 20%, the proportional arrangement of glucose 2 ~ 3%, agar 2 ~ 3%, water 1000ml, PH nature, divides and is filled in test tube, mixes rear high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min; Sterilizing finishes to take out test tube after 10 ~ 15min, is put into inclined-plane, and slant medium is gone to constant temperature culture 18 ~ 20h in 28 ~ 36 DEG C of incubators, without bacterium colony grow can take out for subsequent use;
B: the preparation of shaking flask medium
According to mass percent potato 18 ~ 20%, the proportional arrangement of glucose 2 ~ 3%, water 1000ml, PH nature, mixes rear high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min, puts into aseptic inoculation chamber after cooling and treat that inoculation uses;
C: the preparation of seed tank culture base
According to mass percent glucose 5 ~ 7%, soluble starch 2 ~ 3%, corn flour 3 ~ 5%, soybean meal 0.7 ~ 1.5%, yeast extract 1 ~ 2%, potassium dihydrogen phosphate 0.1 ~ 0.3%, magnesium sulfate 0.08 ~ 0.12%, add water to 100L, then carries out high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min; After sterilizing finishes, in the time that medium temperature is down to 27 ~ 35 DEG C, puts into aseptic inoculation chamber and treat inoculation use;
D: the preparation of fermentation tank culture medium
According to mass percent glucose 5 ~ 7%, soluble starch 2 ~ 3%, corn flour 3 ~ 5%, soybean meal 0.7 ~ 1.5%, yeast extract 1 ~ 2%, potassium dihydrogen phosphate 0.1 ~ 0.3%, magnesium sulfate 0.08 ~ 0.12%, add water to 1000L, retort is heated, when rising to 100 ~ 105 DEG C, temperature carries out an exhaust, while continuing to be warming up to 121 ~ 123 DEG C, close respirator and import and export switch, make mixed culture medium at 121 ~ 123 DEG C, sterilizing 30 ~ 40min in 0.15 ~ 0.17MPa environment, sterilizing is complete, close heating system make mixed culture medium be cooled to 25 ~ 28 DEG C of heat preservation for standby use put into aseptic inoculation chamber treat inoculation use,
Activated spawn step comprises:
(1) select Hericium erinaceus bacterial strain, be inoculated into slant medium and cultivate, condition of culture is: 26 ~ 28 DEG C, 2 ~ 3 days, treat that inclined-plane mycelia covers with, as mass-produced female kind;
(2) mother who upper bevel is cultivated plants and is seeded to shaking flask medium, and 26 ~ 28 DEG C are cultured to the circular shaking table that is placed on of bacterium block length, and 28 ~ 30 DEG C of cultivation 60 ~ 70h of constant temperature obtain liquid culture bacterial classification after having mycelium pellet to form.
In seed tank culture step, liquid culture bacterial classification being inoculated in the seed tank culture base having prepared by 10%, is 0.3～0.5 (m in ventilation
3/ m
3s), speed of agitator is 180r/min, and temperature is that under the condition of 26 ~ 28 DEG C, lucifuge is cultivated 2d, after fermentation the seed of secondary seed tank without miscellaneous bacteria, the mycelium smell delicate fragrance of medium, mycelium ball is many, slightly thickness.
Fermentation tank expands in incubation step, in the fermentation tank culture medium that the bacterial classification of seed tank culture has been prepared by 8% inoculation, is 0.3～0.5 (m in ventilation
3/ m
3s), speed of agitator is 180r/min, and temperature is that under the condition of 26 ~ 28 DEG C, lucifuge is cultivated 5d.
In enzymolysis step, mycelia liquid after fermentation is warming up to 50 DEG C, adds 0.3% cellulase by fermented liquid mass ratio, enzymolysis 90min.
In external circulation evaporator concentration step, its evaporating temperature is 70 DEG C, is under condition more than 0.08MPa in vacuum, makes solution solid content after concentrated in 25% left and right.
In homogenizing step, the liquid after concentrated is carried out to homogeneous by homogenizer under the condition of pressure 150MPa, make the solid content in solution reach refinement more, mix more uniformly.
In spraying drying steps, be 150 DEG C at EAT, leaving air temp is under the condition of 80～90 DEG C, the Ganoderma lucidum mycelium liquid after concentrated, homogeneous to be dried, and the water content of final dry powder is less than below 7%, gets product.
The invention has the advantages that:
1, adopt biotechnology (liquid deep layer fermenting method) to carry out suitability for industrialized production hericium mycelium powder, have with short production cyclely, take up an area less, output is high, polysaccharide yield high.
2, the hericium mycelium mycelium of producing by liquid deep layer fermenting, is of high nutritive value, and has unique local flavor, has more edibility.
3, adopt biology enzyme (cellulase) to process the hericium mycelium liquid after fermentation, refinement the fiber of mycelia, the follow-up concentrated and homogeneous link of being more convenient for, the yield of finished product is higher.The Ganoderma lucidum mycelium powder quality homogeneous that the dry supervisor post processing of spraying is obtained and more stable, is easier to preserve.
4, adopt the dry hericium mycelium powder processed of spraying, make the uniformity, dissolubility and the mobility of product better, more stable, be easier to preserve; And production process is simplified, operation is controlled easy, is easier to the large-scale production in food industry.
embodiment
Bacterial classification source in following instance is as follows: hericium erinaceus (CAHHFT-7010), (the Chinese Academy of Agricultural Sciences's Hericium erinaceus kind);
Example 1
1, prepare medium
A: the preparation of slant medium
According to mass percent potato 18%, the proportional arrangement of glucose 2%, agar 2%, water 1000ml, PH nature, divides and is filled in test tube, mixes rear high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min; Sterilizing finishes to take out test tube after 10 ~ 15min, is put into inclined-plane, and slant medium is gone to constant temperature culture 18 ~ 20h in 28 ~ 36 DEG C of incubators, without bacterium colony grow can take out for subsequent use;
B: the preparation of shaking flask medium
According to mass percent potato 18%, the proportional arrangement of glucose 2%, water 1000ml, PH nature, mixes rear high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min, puts into aseptic inoculation chamber after cooling and treat that inoculation uses;
C: the preparation of seed tank culture base
Take glucose 5kg, soluble starch 2kg, corn flour 3kg, soybean meal 3kg, yeast extract 1kg, potassium dihydrogen phosphate 0.1kg, magnesium sulfate 0.08kg according to quality, add water to 100L, then carry out high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min; After sterilizing finishes, in the time that medium temperature is down to 27 ~ 35 DEG C, puts into aseptic inoculation chamber and treat inoculation use;
D: the preparation of fermentation tank culture medium
Take glucose 50kg, soluble starch 20kg, corn flour 30kg, soybean meal 30kg, yeast extract 10kg, potassium dihydrogen phosphate 1kg, magnesium sulfate 0.8kg according to quality, add water to 1000L, retort is heated, when rising to 100 ~ 105 DEG C, temperature carries out an exhaust, while continuing to be warming up to 121 ~ 123 DEG C, close respirator and import and export switch, make mixed culture medium sterilizing 30 ~ 40min in 121 ~ 123 DEG C, 0.15 ~ 0.17MPa environment, sterilizing is complete, close heating system make mixed culture medium be cooled to 25 ~ 28 DEG C of heat preservation for standby use put into aseptic inoculation chamber treat inoculation use;
2, the activation of bacterial classification
(1) select Hericium erinaceus bacterial strain, be inoculated into slant medium and cultivate, condition of culture is: 26 ~ 28 DEG C, 2 ~ 3 days, treat that inclined-plane mycelia covers with, as mass-produced female kind;
(2) mother who upper bevel is cultivated plants and is seeded to shaking flask medium, and 26 ~ 28 DEG C are cultured to the circular shaking table that is placed on of bacterium block length, and 28 ~ 30 DEG C of cultivation 60 ~ 70h of constant temperature obtain liquid culture bacterial classification after having mycelium pellet to form.
3, inoculation and expansion fermented and cultured
In seed tank culture step, liquid culture bacterial classification being inoculated in the seed tank culture base having prepared by 10%, is 0.3～0.5 (m in ventilation
3/ m
3s), speed of agitator is 180r/min, and temperature is that under the condition of 26 ~ 28 DEG C, lucifuge is cultivated 2d, after fermentation the seed of secondary seed tank without miscellaneous bacteria, the mycelium smell delicate fragrance of medium, mycelium ball is many, slightly thickness.
Fermentation tank expands in incubation step, in the fermentation tank culture medium that the bacterial classification of seed tank culture has been prepared by 8% inoculation, is 0.3～0.5 (m in ventilation
3/ m
3s), speed of agitator is 180r/min, and temperature is that under the condition of 26 ~ 28 DEG C, lucifuge is cultivated 5d.
4, enzymolysis processing zymotic fluid
In enzymolysis step, mycelia liquid after fermentation is warming up to 50 DEG C, adds 0.3% cellulase by fermented liquid mass ratio, enzymolysis 90min.
5, concentrated
In external circulation evaporator concentration step, its evaporating temperature is 70 DEG C, is under condition more than 0.08MPa in vacuum, makes solution solid content after concentrated in 25% left and right.
6, homogeneous
In homogenizing step, the liquid after concentrated is carried out to homogeneous by homogenizer under the condition of pressure 150MPa, make the solid content in solution reach refinement more, mix more uniformly.
7, spraying is dry
In spraying drying steps, be 150 DEG C at EAT, leaving air temp is under the condition of 80～90 DEG C, the Ganoderma lucidum mycelium liquid after concentrated, homogeneous to be dried, and the water content of final dry powder is less than below 7%, gets product.
Example 2
1, prepare medium
A: the preparation of slant medium
According to mass percent potato 19%, the proportional arrangement of glucose 2.5%, agar 2.5%, water 1000ml, PH nature, divides and is filled in test tube, mixes rear high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min; Sterilizing finishes to take out test tube after 10 ~ 15min, is put into inclined-plane, and slant medium is gone to constant temperature culture 18 ~ 20h in 28 ~ 36 DEG C of incubators, without bacterium colony grow can take out for subsequent use;
B: the preparation of shaking flask medium
According to mass percent potato 19%, the proportional arrangement of glucose 2.5%, water 1000ml, PH nature, mixes rear high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min, puts into aseptic inoculation chamber after cooling and treat that inoculation uses;
C: the preparation of seed tank culture base
Take glucose 6kg, soluble starch 2.5kg, corn flour 4kg, soybean meal 4kg, yeast extract 1.5kg according to quality, potassium dihydrogen phosphate 0.2kg, magnesium sulfate 0.1kg, add water to 100L, then carries out high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min; After sterilizing finishes, in the time that medium temperature is down to 27 ~ 35 DEG C, puts into aseptic inoculation chamber and treat inoculation use;
D: the preparation of fermentation tank culture medium
Take glucose 60kg, soluble starch 25kg, corn flour 40kg, soybean meal 40kg, yeast extract 15kg, potassium dihydrogen phosphate 2kg, magnesium sulfate 1kg according to quality, add water to 1000L, retort is heated, when rising to 100 ~ 105 DEG C, temperature carries out an exhaust, while continuing to be warming up to 121 ~ 123 DEG C, close respirator and import and export switch, make mixed culture medium sterilizing 30 ~ 40min in 121 ~ 123 DEG C, 0.15 ~ 0.17MPa environment, sterilizing is complete, close heating system make mixed culture medium be cooled to 25 ~ 28 DEG C of heat preservation for standby use put into aseptic inoculation chamber treat inoculation use;
2, the activation of bacterial classification
(1) select Hericium erinaceus bacterial strain, be inoculated into slant medium and cultivate, condition of culture is: 26 ~ 28 DEG C, 2 ~ 3 days, treat that inclined-plane mycelia covers with, as mass-produced female kind;
(2) mother who upper bevel is cultivated plants and is seeded to shaking flask medium, and 26 ~ 28 DEG C are cultured to the circular shaking table that is placed on of bacterium block length, and 28 ~ 30 DEG C of cultivation 60 ~ 70h of constant temperature obtain liquid culture bacterial classification after having mycelium pellet to form.
3, inoculation and expansion fermented and cultured
In seed tank culture step, liquid culture bacterial classification being inoculated in the seed tank culture base having prepared by 10%, is 0.3～0.5 (m in ventilation
3/ m
3s), speed of agitator is 180r/min, and temperature is that under the condition of 26 ~ 28 DEG C, lucifuge is cultivated 2d, after fermentation the seed of secondary seed tank without miscellaneous bacteria, the mycelium smell delicate fragrance of medium, mycelium ball is many, slightly thickness.
Fermentation tank expands in incubation step, in the fermentation tank culture medium that the bacterial classification of seed tank culture has been prepared by 8% inoculation, is 0.3～0.5 (m in ventilation
3/ m
3s), speed of agitator is 180r/min, and temperature is that under the condition of 26 ~ 28 DEG C, lucifuge is cultivated 5d.
4, enzymolysis processing zymotic fluid
In enzymolysis step, mycelia liquid after fermentation is warming up to 50 DEG C, adds 0.3% cellulase by fermented liquid mass ratio, enzymolysis 90min.
5, concentrated
In external circulation evaporator concentration step, its evaporating temperature is 70 DEG C, is under condition more than 0.08MPa in vacuum, makes solution solid content after concentrated in 25% left and right.
6, homogeneous
In homogenizing step, the liquid after concentrated is carried out to homogeneous by homogenizer under the condition of pressure 150MPa, make the solid content in solution reach refinement more, mix more uniformly.
7, spraying is dry
In spraying drying steps, be 150 DEG C at EAT, leaving air temp is under the condition of 80～90 DEG C, the Ganoderma lucidum mycelium liquid after concentrated, homogeneous to be dried, and the water content of final dry powder is less than below 7%, gets product.
Example 3
1, prepare medium
A: the preparation of slant medium
According to mass percent potato 20%, the proportional arrangement of glucose 3%, agar 3%, water 1000ml, PH nature, divides and is filled in test tube, mixes rear high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min; Sterilizing finishes to take out test tube after 10 ~ 15min, is put into inclined-plane, and slant medium is gone to constant temperature culture 18 ~ 20h in 28 ~ 36 DEG C of incubators, without bacterium colony grow can take out for subsequent use;
B: the preparation of shaking flask medium
According to mass percent potato 20%, the proportional arrangement of glucose 3%, water 1000ml, PH nature, mixes rear high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min, puts into aseptic inoculation chamber after cooling and treat that inoculation uses;
C: the preparation of seed tank culture base
Take grape 7kg, soluble starch 3kg, corn flour 5kg, soybean meal 5kg, yeast extract 2kg according to quality, potassium dihydrogen phosphate 0.3kg, magnesium sulfate 0.12kg, add water to 100L, then carries out high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, and 30 ~ 40min; After sterilizing finishes, in the time that medium temperature is down to 27 ~ 35 DEG C, puts into aseptic inoculation chamber and treat inoculation use;
D: the preparation of fermentation tank culture medium
Take glucose 70kg, soluble starch 30kg, corn flour 50kg, soybean meal 50kg, yeast extract 20kg, potassium dihydrogen phosphate 3kg, magnesium sulfate 1.2kg according to quality, add water to 1000L, retort is heated, when rising to 100 ~ 105 DEG C, temperature carries out an exhaust, while continuing to be warming up to 121 ~ 123 DEG C, close respirator and import and export switch, make mixed culture medium sterilizing 30 ~ 40min in 121 ~ 123 DEG C, 0.15 ~ 0.17MPa environment, sterilizing is complete, close heating system make mixed culture medium be cooled to 25 ~ 28 DEG C of heat preservation for standby use put into aseptic inoculation chamber treat inoculation use;
2, the activation of bacterial classification
(1) select Hericium erinaceus bacterial strain, be inoculated into slant medium and cultivate, condition of culture is: 26 ~ 28 DEG C, 2 ~ 3 days, treat that inclined-plane mycelia covers with, as mass-produced female kind;
(2) mother who upper bevel is cultivated plants and is seeded to shaking flask medium, and 26 ~ 28 DEG C are cultured to the circular shaking table that is placed on of bacterium block length, and 28 ~ 30 DEG C of cultivation 60 ~ 70h of constant temperature obtain liquid culture bacterial classification after having mycelium pellet to form.
3, inoculation and expansion fermented and cultured
In seed tank culture step, liquid culture bacterial classification being inoculated in the seed tank culture base having prepared by 10%, is 0.3～0.5 (m in ventilation
3/ m
3s), speed of agitator is 180r/min, and temperature is that under the condition of 26 ~ 28 DEG C, lucifuge is cultivated 2d, after fermentation the seed of secondary seed tank without miscellaneous bacteria, the mycelium smell delicate fragrance of medium, mycelium ball is many, slightly thickness.
Fermentation tank expands in incubation step, in the fermentation tank culture medium that the bacterial classification of seed tank culture has been prepared by 8% inoculation, is 0.3～0.5 (m in ventilation
3/ m
3s), speed of agitator is 180r/min, and temperature is that under the condition of 26 ~ 28 DEG C, lucifuge is cultivated 5d.
4, enzymolysis processing zymotic fluid
In enzymolysis step, mycelia liquid after fermentation is warming up to 50 DEG C, adds 0.3% cellulase by fermented liquid mass ratio, enzymolysis 90min.
5, concentrated
In external circulation evaporator concentration step, its evaporating temperature is 70 DEG C, is under condition more than 0.08MPa in vacuum, makes solution solid content after concentrated in 25% left and right.
6, homogeneous
In homogenizing step, the liquid after concentrated is carried out to homogeneous by homogenizer under the condition of pressure 150MPa, make the solid content in solution reach refinement more, mix more uniformly.
7, spraying is dry
In spraying drying steps, be 150 DEG C at EAT, leaving air temp is under the condition of 80～90 DEG C, the Ganoderma lucidum mycelium liquid after concentrated, homogeneous to be dried, and the water content of final dry powder is less than below 7%, gets product.
Claims (6)
1. the auxiliary post processing Hericium erinaceus fermented hypha liquid of biology enzyme prepare the method for hypha powder, it comprises the following steps: prepare medium, activated spawn, inoculation and cultivation, expansion fermentation, enzyme processing, concentrated, homogeneous, spraying drying powder-forming, it is characterized in that, the described step of preparing medium comprises:
a: the preparation of slant medium
according to mass percent potato 18 ~ 20%, the proportional arrangement of glucose 2 ~ 3%, agar 2 ~ 3%, water 1000ml, PH nature, divides and is filled in test tube, mixes rear high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min; Sterilizing finishes to take out test tube after 10 ~ 15min, is put into inclined-plane, and slant medium is gone to constant temperature culture 18 ~ 20h in 28 ~ 36 DEG C of incubators, without bacterium colony grow can take out for subsequent use;
B: the preparation of shaking flask medium
According to mass percent potato 18 ~ 20%, the proportional arrangement of glucose 2 ~ 3%, water 1000ml, PH nature, mixes rear high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min, puts into aseptic inoculation chamber after cooling and treat that inoculation uses;
C: the preparation of seed tank culture base
According to mass percent glucose 5 ~ 7%, soluble starch 2 ~ 3%, corn flour 3 ~ 5%, soybean meal 3 ~ 5%, yeast extract 1 ~ 2%, potassium dihydrogen phosphate 0.1 ~ 0.3%, magnesium sulfate 0.08 ~ 0.12%, add water to 100L, then carries out high-temperature sterilization, sterilising conditions: 121 ~ 123 DEG C, 30 ~ 40min; After sterilizing finishes, in the time that medium temperature is down to 27 ~ 35 DEG C, puts into aseptic inoculation chamber and treat inoculation use;
D: the preparation of fermentation tank culture medium
According to mass percent glucose 5 ~ 7%, soluble starch 2 ~ 3%, corn flour 3 ~ 5%, soybean meal 3 ~ 5%, yeast extract 1 ~ 2%, potassium dihydrogen phosphate 0.1 ~ 0.3%, magnesium sulfate 0.08 ~ 0.12%, add water to 1000L, retort is heated, when rising to 100 ~ 105 DEG C, temperature carries out an exhaust, while continuing to be warming up to 121 ~ 123 DEG C, close respirator and import and export switch, make mixed culture medium at 121 ~ 123 DEG C, sterilizing 30 ~ 40min in 0.15 ~ 0.17MPa environment, sterilizing is complete, close heating system make mixed culture medium be cooled to 25 ~ 28 DEG C of heat preservation for standby use put into aseptic inoculation chamber treat inoculation use,
Described activated spawn step comprises:
Select Hericium erinaceus bacterial strain, be inoculated into slant medium and cultivate, condition of culture is: 26 ~ 28 DEG C, 2 ~ 3 days, treat that inclined-plane mycelia covers with, as mass-produced female kind;
(2) mother who upper bevel is cultivated plants and is seeded to shaking flask medium, and 26 ~ 28 DEG C are cultured to the circular shaking table that is placed on of bacterium block length, and 28 ~ 30 DEG C of cultivation 60 ~ 70h of constant temperature obtain liquid culture bacterial classification after having mycelium pellet to form;
In described seed tank culture step, liquid culture bacterial classification being inoculated in the seed tank culture base having prepared by 10%, is 0.3～0.5 (m in ventilation
3/ m
3s), speed of agitator is 180r/min, and temperature is that under the condition of 26 ~ 28 DEG C, lucifuge is cultivated 2d, after fermentation the seed of secondary seed tank without miscellaneous bacteria, the mycelium smell delicate fragrance of medium, mycelium ball is many, slightly thickness;
Described fermentation tank expands in incubation step, in the fermentation tank culture medium that the bacterial classification of seed tank culture has been prepared by 8% inoculation, is 0.3～0.5 (m in ventilation
3/ m
3s), speed of agitator is 180r/min, and temperature is that under the condition of 26 ~ 28 DEG C, lucifuge is cultivated 5d;
In described enzymolysis processing step, mycelia liquid after fermentation is warming up to 50 DEG C, adds 0.3% cellulase by fermented liquid mass ratio, enzymolysis 90min;
In described concentration step, it is under condition more than 0.08MPa that the temperature of external circulation evaporator is adjusted to 70 DEG C, vacuum, makes solution solid content after concentrated in 25% left and right;
In described homogenizing step, the liquid after concentrated is carried out to homogeneous by homogenizer under the condition of pressure 150MPa, make the solid content in solution reach refinement more, mix more uniformly;
In described spraying drying steps, be 150 DEG C at EAT, leaving air temp is under the condition of 80～90 DEG C, the Ganoderma lucidum mycelium liquid after concentrated, homogeneous to be dried, and the water content of final dry powder is less than below 7%, gets product.
2. the preparation method of hericium mycelium powder according to claim 1, is characterized in that, in described fermentation medium preparation process, uses corn flour and soybean meal as base-material, makes finished product have special local flavor.
3. the preparation method of hericium mycelium powder according to claim 1, is characterized in that, uses cellulase processing in described enzymolysis step, is convenient to refinement and the follow-up homogeneous of product.
4. the preparation method of hericium mycelium powder according to claim 1, is characterized in that, uses external circulation evaporator cryogenic vacuum concentrated in described concentration step.
5. the preparation method of hericium mycelium powder according to claim 1, is characterized in that, uses high-pressure homogeneous refinement mycelia liquid in described homogenizing step.
6. the preparation method of hericium mycelium powder according to claim 1, is characterized in that, uses spraying dry in described drying steps.
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