CN104082025B - A kind of method of cultivating the disease-resistant seedling of color green pepper - Google Patents
A kind of method of cultivating the disease-resistant seedling of color green pepper Download PDFInfo
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Abstract
The invention discloses a kind of method of cultivating the disease-resistant seedling of color green pepper, the method is with corn kernel shot-like particle and wheat bran 1: 1 composition fermentation substrate by volume, by the mode of solid state fermentation, maitake mushroom mycelia is grown in fermentation substrate, obtain the Grifolas frondosa germ fermentation product being rich in maitake mushroom mycelia, by Grifolas frondosa germ fermentation product, the peat composed of rotten mosses and vermiculite prepare seedling medium by volume at 2: 10: 3, carry out point disk seedling growing, color green pepper seedlings root absorbs the grifolan in mycelium, plant is induced to produce resistance to diseases such as phytophthora root rots after grifola frondosus acid and ash tree florigen, the of the right age seedling obtained is the disease-resistant seedling of color green pepper.The present invention can solve " dead " difficult problem caused by Phytophthora Root Rot in the plantation of color green pepper.
Description
Technical field
The present invention relates to a kind of method of cultivating the disease-resistant seedling of color green pepper, belong to growing vegetables technical field.
Background technology
Color dead of green pepper occurs serious in recent years in the Protectorate cultivation such as greenhouse by solar heat; especially occurring more based on the area of color green pepper plantation year after year; seriously have impact on color green pepper and produce, the interests of numerous vegetable growers are suffered huge loss, is urgent problem during current color green pepper produces.
Color dead of green pepper is mainly caused by phytophthora root rot etc. after field planting, and how cultivating color green pepper seedling phytophthora root rot being had to resistance against diseases, is the technical task that current people pay close attention to jointly.
Also do not apply to about bioactive ingredients thing specific to Grifolas frondosa germ the bibliographical information that the cultivation of color green pepper strengthens color pepper plant resistance against diseases at present.
Summary of the invention
The object of the invention is for prior art Problems existing, a kind of method of cultivating the disease-resistant seedling of color green pepper is provided, the seedling medium of maitake mushroom mycelia is rich in by training system, carry out point disk seedling growing, induce plant to produce resistance to phytophthora root rot after grifolan in color green pepper seedlings root absorption mycelium, grifola frondosus acid and ash tree florigen, thus solve during color green pepper produces " dead " difficult problem caused by Phytophthora Root Rot.
For solving above technical problem, the technical solution used in the present invention is as follows: a kind of method of cultivating the disease-resistant seedling of color green pepper, it is characterized in that: with corn kernel shot-like particle and wheat bran 1: 1 composition fermentation substrate by volume, by the mode of solid state fermentation, maitake mushroom mycelia is grown in fermentation substrate, obtain the Grifolas frondosa germ fermentation product being rich in maitake mushroom mycelia, Grifolas frondosa germ fermentation product, the peat composed of rotten mosses and vermiculite are mixed are mixed with seedling medium by volume at 2: 10: 3, carry out substrate seedling, the of the right age seedling obtained is the disease-resistant seedling of color green pepper.Peat composed of rotten mosses diameter 1 ~ 5mm, vermiculite diameter 3 ~ 5mm.
A kind of prioritization scheme, described corn kernel shot-like particle is the particle of the diameter 3 ~ 5mm size be ground into by corn kernel.
Another kind of prioritization scheme, the preparation of described Grifolas frondosa germ fermentation product comprises the following steps:
1) by after activated for slant preservation bacterial classification cultivation, access by potato, glucose, skim milk powder in mass ratio 1000: 20: the 2 semi-synthetic liquid nutrient mediums mixed, cultivate 5 ~ 8 days, obtain grifola frondosus liquid spawn for 18 ~ 25 DEG C;
2) fermentation substrate and water are loaded round according to after the mass ratio mixing of 4: 1 ~ 6: 1,115 ~ 132 DEG C of sterilizings 15 ~ 30 minutes, after sterilizing terminates, are cooled to normal temperature under cleaning condition, for subsequent use;
3) by 10% ~ 15% access grifola frondosus liquid spawn of fermentation substrate quality, under 20 ~ 25 DEG C of conditions, lucifuge was cultivated after 15 ~ 20 days, namely obtained Grifolas frondosa germ fermentation product.
Another prioritization scheme, described seedling-cultivating method is point disk seedling growing.The cave dish selecting each hole volume to be not less than 100ml carries out nursery, ensure that the consumption of the above-mentioned seedling medium of cultivation 1 strain rice shoot is not less than 100ml, after the matrix got ready is loaded cave dish, by bunch planting kind, the color green pepper seed of every bunch planting kind one, puts into seedbed by cave dish after broadcasting and manages routinely.
Another prioritization scheme, described of the right age seedling is 3 ~ 5 leaf 1 heart seedlings.
Adopt above technical scheme, compared with prior art, have the following advantages:
(1) seedling medium of maitake mushroom mycelia is rich in the present invention by training system, carry out point disk seedling growing, plant is induced to produce resisting substance to phytophthora root rot after grifolan in color green pepper seedlings root absorption mycelium, grifola frondosus acid and ash tree florigen, the germ of invasion can be annihilated rapidly, make color green pepper from phytophthora root rot, solve " dead " problem caused by phytophthora root rot being difficult in the plantation of color green pepper prevent and treat.
(2) the color green pepper rice shoot that the present invention cultivates all is better than existing conventional substrate seedling growing process after improving activity of root system, seedling vigorous index and field planting in output.
(3) the color green pepper rice shoot that the present invention cultivates contains multiple resistance material, not only have resistance to phytophthora root rot, and also have resistance in various degree to other color green pepper disease, after field planting, damage by disease and insect occurs few, dosage and number of times reduce, and product is safe green more.
Below in conjunction with embodiment, the invention will be further described.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 day-light greenhouse winter spring stubble cultivates color green pepper, and varieties of plant is red Luo Dan, and September 5 was sowed, and cultivate 4 leaf 1 heart seedlings, field planting on October 15, place is at Shouguang, Shandong green house of vegetables.
Process 1: adopt method of the present invention to carry out point disk seedling growing.Substrate composition is: Grifolas frondosa germ fermentation product, the peat composed of rotten mosses and vermiculite volume ratio 2: 10: 3, the particle diameter 1 ~ 5mm of the peat composed of rotten mosses, and the particle diameter of vermiculite is 3 ~ 5mm.
The making step of Grifolas frondosa germ fermentation product comprises: after activated for slant preservation bacterial classification cultivation, access is by potato, glucose, skim milk powder in mass ratio 1000: 20: the 2 semi-synthetic liquid nutrient mediums formed, cultivate 5 ~ 8 days, make grifola frondosus liquid spawn for 18 ~ 25 DEG C; Corn kernel is ground into the particle of particle diameter 3 ~ 5mm size, with corn particle and wheat bran by 1: 1 volume ratio be mixed into fermentation substrate; Above-mentioned fermentation substrate and water are loaded round according to after the mass ratio mixing of 4: 1 ~ 6: 1, and 115 ~ 132 DEG C of sterilizings 15 ~ 30 minutes, after sterilizing terminates, are cooled to normal temperature under cleaning condition, for subsequent use; By 10% ~ 15% access grifola frondosus liquid spawn of fermentation substrate quality, under 20 ~ 25 DEG C of conditions, lucifuge was cultivated after 15 ~ 20 days, namely obtained Grifolas frondosa germ fermentation product.
Process 2: adopt the seedling medium of EM bacterium fermentation product to carry out point disk seedling growing.Substrate composition is: EM bacterium fermentation product, the peat composed of rotten mosses and vermiculite volume ratio 2: 10: 3, and the particle diameter of the peat composed of rotten mosses is 1 ~ 5mm, and the particle diameter of vermiculite is 3 ~ 5mm.
The making step of EM bacterium fermentation product comprises: particle corn kernel being ground into particle diameter 3 ~ 5mm size, with corn particle and wheat bran by 1: 1 volume ratio be mixed into fermentation substrate; With active bacteria number>=2 × 10
9the EM stoste 2.5g of cfu/g is watered 1kg mixing and is made into EM bacterium liquid, and the above-mentioned fermentation substrate of every 100kg sprays EM bacterium liquid 3kg, mixes, adds water to water content 65%; Covered with plastic film insulation, moisturizing, carry out pile fermentation, period suitably breathe freely, moisturizing and stirring under 30 ~ 40 DEG C of conditions.After 8 ~ 10 days after stack retting, namely obtain EM bacterium fermentation product.
Process 3: adopt conventional peat composed of rotten mosses seedling medium to carry out point disk seedling growing.Substrate composition is: the peat composed of rotten mosses, vermiculite volume ratio 4: 1, the particle diameter 1 ~ 5mm of the peat composed of rotten mosses, and the particle diameter of vermiculite is 3 ~ 5mm.
Above-mentioned each process all select hole volume be 100ml cave dish carry out nursery, the consumption of every strain rice shoot matrix is 100ml, during rice shoot 3 leaf 1 heart, each process is got 10 strains at random and is measured seedling vigorous index, 50 strain inoculation phytophthora root rot germs are got in each process simultaneously at random, within 20 days, observe seedling incidence afterwards; Field planting during 4 leaf 1 heart, statistics output, a situation arises to observe other Major Diseases in whole vegetative period.
Through test, the seedling seedling vigorous index that the present invention cultivates is 0.0369, EM bacterium fermentation product seedling medium is 0.0298, and conventional peat composed of rotten mosses seedling medium is 0.0268, the present invention comparatively EM bacterium fermentation product seedling medium, conventional peat composed of rotten mosses seedling medium improves 23.8%, 37.7% respectively, and difference reaches significance level.
The seedling inoculation phytophthora root rot germ sequela rate that the present invention cultivates is 8%, the seedling inoculation phytophthora root rot germ sequela rate that EM bacterium fermentation product seedling medium is cultivated is 90%, and the seedling inoculation phytophthora root rot germ sequela rate that conventional peat composed of rotten mosses seedling medium is cultivated is 100%.
667m after the Seeding planting that the present invention cultivates
2output is 11605.6kg, EM bacterium fermentation product seedling medium is 9971.5kg, and conventional peat composed of rotten mosses seedling medium is 9225.1kg, and the present invention comparatively EM bacterium fermentation product seedling medium, conventional peat composed of rotten mosses seedling medium improves 16.4%, 25.8% respectively, and difference reaches significance level.
Have no bacterial leaf spot after the Seeding planting that the present invention cultivates to occur, and EM bacterium fermentation product seedling medium and the conventional peat composed of rotten mosses seedling medium bacterial leaf spot incidence of disease are respectively 8.6% and 12.5%.
After the Seeding planting that the present invention cultivates, gray mold incidence is 4.7%, and EM bacterium fermentation product seedling medium and the conventional peat composed of rotten mosses seedling medium gray mold incidence of disease are respectively 11.5% and 17.1%.
Embodiment 2 greenhouse by solar heat is got over summer stubble and is cultivated color green pepper, and varieties of plant is that century is red, and May 5 was sowed, and cultivate 4 leaf 1 heart seedlings, field planting on June 10, place is at Shouguang, Shandong green house of vegetables.
Process 1: adopt method of the present invention to carry out point disk seedling growing.Substrate composition is: Grifolas frondosa germ fermentation product, the peat composed of rotten mosses and vermiculite volume ratio 2: 10: 3, the particle diameter 1 ~ 5mm of the peat composed of rotten mosses, and the particle diameter of vermiculite is 3 ~ 5mm.
The making step of Grifolas frondosa germ fermentation product comprises: after activated for slant preservation bacterial classification cultivation, access is by potato, glucose, skim milk powder in mass ratio 1000: 20: the 2 semi-synthetic liquid nutrient mediums formed, cultivate 7 days, make grifola frondosus liquid spawn for 20 DEG C; Corn kernel is ground into the particle of particle diameter 3 ~ 5mm size, with corn particle and wheat bran by 1: 1 volume ratio be mixed into fermentation substrate; Above-mentioned fermentation substrate and water are loaded round according to after the mass ratio mixing of 5: 1, and 120 DEG C of sterilizings 25 minutes, after sterilizing terminates, are cooled to normal temperature under cleaning condition, for subsequent use; By 12% access grifola frondosus liquid spawn of fermentation substrate quality, under 25 DEG C of conditions, after lucifuge cultivates 15 days, namely obtain Grifolas frondosa germ fermentation product.
Process 2: adopt the seedling medium of EM bacterium fermentation product to carry out point disk seedling growing.Substrate composition is: EM bacterium fermentation product, the peat composed of rotten mosses and vermiculite volume ratio 2: 10: 3, and the particle diameter of the peat composed of rotten mosses is 1 ~ 5mm, and the particle diameter of vermiculite is 3 ~ 5mm.
The making step of EM bacterium fermentation product comprises: particle corn kernel being ground into particle diameter 3 ~ 5mm size, with corn particle and wheat bran by 1: 1 volume ratio be mixed into fermentation substrate; With active bacteria number>=2 × 10
9the EM stoste 2.5g of cfu/g is watered 1kg mixing and is made into EM bacterium liquid, and the above-mentioned fermentation substrate of every 100kg sprays EM bacterium liquid 3kg, mixes, adds water to water content 65%; Covered with plastic film insulation, moisturizing, carry out pile fermentation, period suitably breathe freely, moisturizing and stirring under 30 ~ 40 DEG C of conditions.After 8 ~ 10 days after stack retting, namely obtain EM bacterium fermentation product.
Process 3: adopt conventional peat composed of rotten mosses seedling medium to carry out point disk seedling growing.Substrate composition is: the peat composed of rotten mosses, vermiculite volume ratio 4: 1, and the particle diameter of the peat composed of rotten mosses is 1 ~ 5mm, and the particle diameter of vermiculite is 3 ~ 5mm.
Above-mentioned each process all select hole volume be 100ml cave dish carry out nursery, the consumption of every strain rice shoot matrix is 100ml, during rice shoot 3 leaf 1 heart, each process is got 10 strains at random and is measured improving activity of root system, 50 strain inoculation phytophthora root rot germs are got in each process simultaneously at random, within 20 days, observe seedling incidence afterwards; Field planting during 4 leaf 1 heart, statistics output, a situation arises to observe other Major Diseases in whole vegetative period.
Through test, the seedlings root vigor that the present invention cultivates is 84.46mgg
-1h
-1, EM bacterium fermentation product seedling medium is 78.69mgg
-1h
-1, conventional peat composed of rotten mosses seedling medium is 71.52mgg
-1h
-1, the present invention comparatively EM bacterium fermentation product seedling medium, conventional peat composed of rotten mosses seedling medium improves 7.3%, 18.1% respectively, and difference reaches significance level.
The seedling inoculation phytophthora root rot germ sequela rate that the present invention cultivates is 12%, the seedling inoculation phytophthora root rot germ sequela rate that EM bacterium fermentation product seedling medium is cultivated is 98%, and the seedling inoculation phytophthora root rot germ sequela rate that conventional peat composed of rotten mosses seedling medium is cultivated is 100%.
667m after the Seeding planting that the present invention cultivates
2output is 6157.2kg, EM bacterium fermentation product seedling medium is 5391.8kg, and conventional peat composed of rotten mosses seedling medium is 4893.5kg, and the present invention comparatively EM bacterium fermentation product seedling medium, conventional peat composed of rotten mosses seedling medium improves 14.2%, 25.8% respectively, and difference reaches significance level.
After the Seeding planting that the present invention cultivates, mosaic virus incidence is 2.8%, and EM bacterium fermentation product seedling medium and conventional peat composed of rotten mosses seedling medium mosaic virus incidence are respectively 7.5% and 11.2%.
Claims (5)
1. cultivate the method for the disease-resistant seedling of color green pepper for one kind, it is characterized in that: with corn kernel shot-like particle and wheat bran 1:1 composition fermentation substrate by volume, by the mode of solid state fermentation, maitake mushroom mycelia is grown in fermentation substrate, obtain the Grifolas frondosa germ fermentation product being rich in maitake mushroom mycelia, by Grifolas frondosa germ fermentation product, the peat composed of rotten mosses and vermiculite by volume 2:10:3 Homogeneous phase mixing prepare seedling medium, carry out substrate seedling, the of the right age seedling obtained is the disease-resistant seedling of color green pepper.
2. method according to claim 1, is characterized in that: described corn kernel shot-like particle is the particle of the diameter 3 ~ 5mm size be ground into by corn kernel.
3. method according to claim 1, is characterized in that: the preparation of described Grifolas frondosa germ fermentation product comprises the following steps:
1) by after activated for slant preservation bacterial classification cultivation, access by potato, glucose, the skim milk powder semi-synthetic liquid nutrient medium that 1000:20:2 mixes in mass ratio, cultivate 5 ~ 8 days, obtain grifola frondosus liquid spawn for 18 ~ 25 DEG C;
2) fermentation substrate and water are loaded round according to after the mass ratio mixing of 4:1 ~ 6:1,115 ~ 132 DEG C of sterilizings 15 ~ 30 minutes, after sterilizing terminates, are cooled to normal temperature under cleaning condition, for subsequent use;
3) by step 2) in 10% ~ 15% access grifola frondosus liquid spawn of mixture quality of fermentation substrate and water, under 20 ~ 25 DEG C of conditions, lucifuge was cultivated after 15 ~ 20 days, namely obtained Grifolas frondosa germ fermentation product.
4. method according to claim 1, is characterized in that: described seedling-cultivating method is point disk seedling growing.
5. method according to claim 1, is characterized in that: described of the right age seedling is 3 ~ 5 leaf 1 heart seedlings.
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| CN105123471B (en) * | 2015-08-28 | 2018-03-06 | 寿光市新世纪种苗有限公司 | A kind of breeding method of tomato health seedling |
| CN105052515B (en) * | 2015-08-28 | 2017-09-22 | 寿光恒蔬无疆农业发展集团有限公司 | A kind of breeding method of the healthy seedling of color green pepper |
| CN106008027A (en) * | 2016-06-30 | 2016-10-12 | 江苏挚信花卉发展有限公司 | Green environment-friendly nutrient solution beneficial to plant growth |
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| CN101747106A (en) * | 2009-12-18 | 2010-06-23 | 上海市农业科学院 | Grifola frondosa culture material |
| CN101855984A (en) * | 2010-03-29 | 2010-10-13 | 中国农业科学院蔬菜花卉研究所 | Pepper seedling raising medium and preparation method thereof |
| CN103704106A (en) * | 2013-12-24 | 2014-04-09 | 南京农业大学 | Vinegar residue biological active substrate used for chili seedling growing and cultivation and preparing method thereof |
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Patent Citations (3)
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| CN101747106A (en) * | 2009-12-18 | 2010-06-23 | 上海市农业科学院 | Grifola frondosa culture material |
| CN101855984A (en) * | 2010-03-29 | 2010-10-13 | 中国农业科学院蔬菜花卉研究所 | Pepper seedling raising medium and preparation method thereof |
| CN103704106A (en) * | 2013-12-24 | 2014-04-09 | 南京农业大学 | Vinegar residue biological active substrate used for chili seedling growing and cultivation and preparing method thereof |
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Inventor after: Hu Yongjun Inventor after: Tian Subo Inventor after: Sang Yizhen Inventor after: Wang Mingqin Inventor before: Hu Yongjun Inventor before: Tian Subo |
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Effective date of registration: 20160216 Address after: 262700, Shandong, Shouguang City, Weifang province the Yellow Sea northbound head Applicant after: Shouguang New Century Seed Co., Ltd. Address before: 262700 Shandong city in Weifang Province, Shouguang City Business District 5 Building 2 floor B block Agriculture Bureau Applicant before: Hu Yongjun |
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Effective date of registration: 20170707 Address after: 262702 Shandong province Shouguang Wenjia streets Mulberry Village North Patentee after: Shouguang constant vegetable Agriculture Development Group Co., Ltd. Address before: 262700, Shandong, Shouguang City, Weifang province the Yellow Sea northbound head Patentee before: Shouguang New Century Seed Co., Ltd. |