CN105052515B - A kind of breeding method of the healthy seedling of color green pepper - Google Patents
A kind of breeding method of the healthy seedling of color green pepper Download PDFInfo
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- CN105052515B CN105052515B CN201510538653.XA CN201510538653A CN105052515B CN 105052515 B CN105052515 B CN 105052515B CN 201510538653 A CN201510538653 A CN 201510538653A CN 105052515 B CN105052515 B CN 105052515B
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- 235000008534 Capsicum annuum var annuum Nutrition 0.000 title claims abstract description 39
- 240000008384 Capsicum annuum var. annuum Species 0.000 title claims abstract description 39
- 238000009395 breeding Methods 0.000 title claims abstract description 9
- 241000196324 Embryophyta Species 0.000 claims abstract description 36
- 239000007864 aqueous solution Substances 0.000 claims abstract description 24
- 241000894006 Bacteria Species 0.000 claims abstract description 19
- 241000233866 Fungi Species 0.000 claims abstract description 15
- 239000005819 Potassium phosphonate Substances 0.000 claims abstract description 14
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 10
- 239000010451 perlite Substances 0.000 claims abstract description 9
- 235000019362 perlite Nutrition 0.000 claims abstract description 9
- 239000010455 vermiculite Substances 0.000 claims abstract description 9
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 9
- FQKMRXHEIPOETF-UHFFFAOYSA-N F.OP(O)(O)=O Chemical compound F.OP(O)(O)=O FQKMRXHEIPOETF-UHFFFAOYSA-N 0.000 claims abstract description 8
- 241001582888 Lobus Species 0.000 claims abstract description 6
- 201000010099 disease Diseases 0.000 claims description 44
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 44
- 235000013399 edible fruits Nutrition 0.000 claims description 24
- 240000008042 Zea mays Species 0.000 claims description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 15
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 15
- 235000005822 corn Nutrition 0.000 claims description 15
- 238000000855 fermentation Methods 0.000 claims description 15
- 230000004151 fermentation Effects 0.000 claims description 15
- 239000002245 particle Substances 0.000 claims description 15
- 239000000758 substrate Substances 0.000 claims description 15
- 235000015097 nutrients Nutrition 0.000 claims description 13
- 239000000575 pesticide Substances 0.000 claims description 13
- 239000000126 substance Substances 0.000 claims description 13
- 241000233614 Phytophthora Species 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 10
- KDWVHRNGTQBVEN-UHFFFAOYSA-N tripotassium;phosphite Chemical compound [K+].[K+].[K+].[O-]P([O-])[O-] KDWVHRNGTQBVEN-UHFFFAOYSA-N 0.000 claims description 10
- 239000003337 fertilizer Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000005986 Prohexadione Substances 0.000 claims description 5
- 244000061456 Solanum tuberosum Species 0.000 claims description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 5
- 239000011575 calcium Substances 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 235000001727 glucose Nutrition 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 230000007226 seed germination Effects 0.000 claims description 5
- 235000020183 skimmed milk Nutrition 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 4
- 208000036142 Viral infection Diseases 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 85
- 235000009566 rice Nutrition 0.000 abstract description 85
- 230000012010 growth Effects 0.000 abstract description 54
- 230000000694 effects Effects 0.000 abstract description 11
- 230000004069 differentiation Effects 0.000 abstract description 9
- 208000035240 Disease Resistance Diseases 0.000 abstract description 6
- YXXXKCDYKKSZHL-UHFFFAOYSA-M dipotassium;dioxido(oxo)phosphanium Chemical compound [K+].[K+].[O-][P+]([O-])=O YXXXKCDYKKSZHL-UHFFFAOYSA-M 0.000 abstract description 4
- NLKUPINTOLSSLD-UHFFFAOYSA-L calcium;4-(1-oxidopropylidene)-3,5-dioxocyclohexane-1-carboxylate Chemical compound [Ca+2].CCC([O-])=C1C(=O)CC(C([O-])=O)CC1=O NLKUPINTOLSSLD-UHFFFAOYSA-L 0.000 abstract description 3
- 231100000331 toxic Toxicity 0.000 abstract description 3
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- 238000000034 method Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 238000007796 conventional method Methods 0.000 description 6
- 238000012549 training Methods 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 235000013311 vegetables Nutrition 0.000 description 5
- 238000013329 compounding Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 239000005556 hormone Substances 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 238000012913 prioritisation Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000010496 root system development Effects 0.000 description 2
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of breeding method of the healthy seedling of color green pepper, will be enriched in Cordceps militaris bacterium fermentate, turf, vermiculite and the perlite of scarlet caterpiller fungus mycelia by volume 1：5：2：2 uniform mixed preparing seedling mediums, carry out hole plate seedling growth；Color green pepper sprout to cotyledon deploy when every plant pour and apply concentration for 30mgkg‑1The 8ml of the potassium phosphite aqueous solution 5；1st lobus cardiacus dew point pours to every plant during 11 heart of leaf and applies concentration for 20mgkg‑1The 10ml of the Prohexadione calcium aqueous solution 8；Every plant is poured and applies concentration for 100mgkg during 21 heart of leaf‑1The 15ml of the toxic fluoride phosphate aqueous solution 12；Every plant is poured and applies concentration for 75mgkg during 31 heart of leaf‑1The 15ml of the potassium phosphite aqueous solution 12.The rice shoot that the present invention is brought out better than conventional hole plate seedling growth in terms of root activity, seedling vigorous index, bud differentiation, disease resistance and yield.
Description
Technical field
The present invention relates to a kind of breeding method of color green pepper seedling, specifically, it is related to a kind of method for cultivating the healthy seedling of color green pepper,
Belong to vegetables seedling-raising technique field.
Background technology
Color green pepper hole plate seedling growth is widely used to production.Hole plate seedling growth is to make nursery base with light materials such as turf, vermiculites
Matter, using precision drilling, the modernization nursery system of once-seedling forming.Traditional hole plate seedling growth technology has energy-conservation, saving of labor, province
Power, saving seedbed area, economize on kind, are easy to the advantages of long distance transportation is also allowed for using standardization seedling management technology, fixed
Plant is easily survived, no seedling-slowing stage, and nursery production realizes specialized, mechanization, and commercialization is realized for seedling.
In process of the present invention is realized, inventor has found at least there is problems with the prior art：Traditional color green pepper cave
Disk seedling-raising technique tends to cause rice shoot excessive growth due to factors such as hole plate seedling growth environment, causes the quality of seedlings to decline, main table
Rooting ability after being now colonized for rice shoot is poor, and growth potential is weak, and malformed fruit rate is high（Mainly seedling stage bud differentiation is bad caused）, resist
Characteristic of disease is poor, yields poorly.
The content of the invention
The technical problems to be solved by the invention are to be directed to problem above there is provided a kind of method for cultivating the healthy seedling of color green pepper,
Rice shoot excessive growth can effectively be suppressed, be conducive to nurturing staff, promote root system development, root activity and seedling vigorous index is improved；Promote flower
Bud well breaks up, and reduces malformed fruit rate, improves the commodity of fruit；Strengthen the disease resistance of rice shoot, reduce the use of chemical pesticide
Number of times and consumption；Improve color green pepper yield.
To solve the above problems, the present invention is adopted the following technical scheme that：The breeding method of color green pepper health seedling, by Cordceps militaris bacterium
Fermentate, turf, vermiculite and perlite by volume 1：5：2：2 uniform mixed preparings are rich in the nursery base of scarlet caterpiller fungus mycelia
Hole plate seedling growth is carried out after matter, medium in tray, is comprised the following steps：
1）Color green pepper seed is sowed at 72 hole standard hole trays, and every plant is poured and applies concentration for 30mg when seed germination to cotyledon deploys
kg-1Potassium phosphite aqueous solution 5-8ml；
2）1st lobus cardiacus dew point pours to every plant during 11 heart of leaf and applies concentration for 20mgkg-1Prohexadione calcium aqueous solution 8-
10ml；
3）Every plant is poured and applies concentration for 100mgkg during 21 heart of leaf-1Toxic fluoride phosphate aqueous solution 12-15ml；
4）Every plant is poured and applies concentration for 75mgkg during 31 heart of leaf-1Potassium phosphite aqueous solution 12-15ml.
A kind of prioritization scheme：The Cordceps militaris bacterium fermentate, is prepared from by following steps：
1）The particle for the diameter 3-5mm sizes that corn kernel is ground into, is made corn kernel shot-like particle；
2）With corn kernel shot-like particle and wheat bran by volume 1：1 composition fermentation substrate；
3）After the activated culture of slant preservation strain, access by potato, glucose, skimmed milk power in mass ratio
1000：20：In the 2 semi-synthetic fluid nutrient mediums mixed, 20-28 DEG C is cultivated 10 days, produces Cordceps militaris liquid spawn；
4）By fermentation substrate and water according to 5：Load round after 1 mass ratio mixing, 115-132 DEG C sterilizes 20 points
Clock, after sterilizing terminates, is cooled to normal temperature under cleaning condition, standby；
5）Cordceps militaris liquid spawn is accessed by the 10%-15% of fermentation substrate quality, under the conditions of 20-28 DEG C, lucifuge training
After supporting 8 days, then culture 18 days under 100-150lx illumination condition are transferred to, that is, obtain Cordceps militaris bacterium fermentate, the fermentate is rich in
Scarlet caterpiller fungus mycelia.
Another prioritization scheme：The full elements compounding of the appropriate full dissolubility of seedling medium addition rich in scarlet caterpiller fungus mycelia
Fertilizer, makes seedling medium initial nutrient N, P2O5、K2O content is respectively：50-60mg·kg-1、20-40mg·kg-1、80-
90mg·kg-1, middle micro- appropriate, EC values 1.0-1.5mscm-1。
Further, rice shoot seedling vigorous index is 0.0298-0.0325, improves 22.3%-23.7%；
Rice shoot root activity is 81.2mgg-1·h-1-86.9mg·g-1·h- 1,, improve 20.1%-22.4%；
Malformed fruit rate is 0 after rice shoot field planting；
Field virosis, the phytophthora root rot incidence of disease are 0 after rice shoot field planting；
Chemical pesticide can be reduced in production using 3-5 times, chemical pesticide usage amount reduces more than 40%；
Total output is 4926.8-12258.3kg/ mus after rice shoot field planting, increases production 22.8%-23.0%.
Standard hole tray is many cavities containers for vegetables nursery, is made of plastics, and the size of standard hole tray is long 52cm
× wide 28cm × high 4.5cm.
Using above technical scheme, compared with prior art, with advantages below：
（1）Rice shoot excessive growth can effectively be suppressed, be conducive to nurturing staff, promote root system development, root activity is improved and strong
Seedling index.According to experiment, gained rice shoot seedling vigorous index is 0.0298-0.0325, and more conventional Plug seedling of educating improves 22.3%-23.7%；
Root activity more 81.2mgg-1·h-1-86.9mg·g-1·h- 1,, conventional hole plate seedling growth raising 20.1%-22.4%.
（2）Rice shoot bud differentiation is good, is conducive to improving the commodity of fruit.According to experiment, gained rice shoot color green pepper fruit is abnormal
Shape fruit rate is 0, and conventional Plug seedling color green pepper deformed fruit fruit rate is 9.8%-14.6%.
（3）Rice shoot disease resistance is strong, is conducive to realizing that the decrement of chemical pesticide is used in color green pepper cultivation.According to experiment, gained
Field virosis, the phytophthora root rot incidence of disease are 0 after rice shoot field planting；The incidence of disease of the diseases such as epidemic disease, bacterial leaf spot is also remote
Chemical pesticide can be reduced less than conventional hole plate seedling growth, in production using 3-5 times, chemical pesticide usage amount reduces more than 40%.
（4）Be conducive to improving color green pepper yield.According to experiment, yield is 4926.8-12258.3kg/ mus after the field planting of gained rice shoot,
More conventional hole plate seedling growth improves 22.8%-23.0%.
Selection and the consumption of potassium phosphite, Prohexadione calcium and toxic fluoride phosphate in the present invention, are the biological characteristics according to color green pepper
Property, and result of the test of the present inventor in color green pepper hole plate seedling growth and determine.It is the most frequent field of hole plate seedling growth to water
Operation, potassium phosphite, Prohexadione calcium and pouring for toxic fluoride phosphate are applied and can not increase recruitment with progress of watering.
The raw materials used present invention is to allow what is used in agricultural production, and raw materials market is on sale.
With reference to embodiment, the present invention is described in detail.
Embodiment
The present embodiment is claim and core without limiting the present invention in any way for the ease of understanding the present invention
Content.
Embodiment 1, heliogreenhouse Autumn cultivation color green pepper, varieties of plant is Wei Weier, and sowing on July 6, August is determined on the 14th
Plant, place is in Shandong Shouguang green house of vegetables.If 2 processing, from 72 hole standard hole plate seedling growths, in addition to desired measure,
Other control measures are identical.
Cordceps militaris bacterium fermentate, is prepared from by following steps：
1）The particle for the diameter 3-5mm sizes that corn kernel is ground into, is made corn kernel shot-like particle；
2）With corn kernel shot-like particle and wheat bran by volume 1：1 composition fermentation substrate；
3）After the activated culture of slant preservation strain, access by potato, glucose, skimmed milk power in mass ratio
1000：20：In the 2 semi-synthetic fluid nutrient mediums mixed, 20-28 DEG C is cultivated 10 days, produces Cordceps militaris liquid spawn；
4）By fermentation substrate and water according to 5：Load round after 1 mass ratio mixing, 115-132 DEG C sterilizes 20 points
Clock, after sterilizing terminates, is cooled to normal temperature under cleaning condition, standby；
5）Cordceps militaris liquid spawn is accessed by the 10%-15% of fermentation substrate quality, under the conditions of 20-28 DEG C, lucifuge training
After supporting 8 days, then culture 18 days under 100-150lx illumination condition are transferred to, that is, obtain Cordceps militaris bacterium fermentate.
Processing 1：Using the inventive method nursery
By Cordceps militaris bacterium fermentate, turf, vermiculite and perlite by volume 1：5：2：2 uniform mixing, while addition is suitable
Measure full dissolubility all-element compound fertilizer and prepare the seedling medium rich in scarlet caterpiller fungus mycelia, make seedling medium initial nutrient N, P2O5、
K2O content is respectively：50-60mg·kg-1、20-40mg·kg-1、80-90mg·kg-1, middle micro- appropriate, EC values 1.0-
1.5ms·cm-1, color green pepper hole plate seedling growth is carried out after medium in tray；Every plant is poured and applies concentration when after planting seed germination to cotyledon deploys
For 30mgkg-1Potassium phosphite aqueous solution 5-8ml；1st lobus cardiacus dew point pours to every plant during 11 heart of leaf and applies concentration for 20mg
kg-1Prohexadione calcium aqueous solution 8-10ml；Every plant is poured and applies concentration for 100mgkg during 21 heart of leaf-1Toxic fluoride phosphate aqueous solution 12-
15ml；Every plant is poured and applies concentration for 75mgkg during 31 heart of leaf-1Potassium phosphite aqueous solution 12-15ml.
Processing 2：Conventional method nursery
By 6: 2: the 2 uniform mixing by volume of turf, vermiculite, perlite, while the full elements compounding of the appropriate full dissolubility of addition
Fertilizer prepares seedling medium, makes seedling medium initial nutrient N, P2O5、K2O content is respectively：50-60mg·kg-1、20-40mg·
kg-1、80-90mg·kg-1, middle micro- appropriate, EC values 1.0-1.5mscm-1, color green pepper hole tray is carried out after medium in tray and is educated
Seedling, according to a conventional method spray on plants hormone control rice shoot excessive growth.
It is colonized during above-mentioned each processing 41 heart of leaf of rice shoot, root activity and seedling vigorous index is determined before field planting；Investigated after field planting color
The malformed fruit incidence of green pepper fruit, analyzes rice shoot bud differentiation situation；Investigating field diseases, a situation arises, analyzes the disease-resistant of rice shoot
Property；Count color green pepper total output.
Through experiment, the rice shoot seedling vigorous index that the present invention is cultivated is 0.0307, and the rice shoot strong sprout that conventional hole plate seedling growth is brought out refers to
Number is 0.0251, and more conventional hole plate seedling growth of the invention improves 22.3%, and difference reaches the level of signifiance.
The rice shoot root activity that the present invention is cultivated is 81.2mgg-1·h-1, the rice shoot root system that conventional hole plate seedling growth is cultivated
Vigor is 67.6mgg-1·h-1, more conventional hole plate seedling growth of the invention improves 20.1%, significant difference.
The rice shoot fruit fruit malformed fruit rate that the present invention is cultivated is 0, and the rice shoot malformed fruit that conventional hole plate seedling growth is cultivated
Rate is 9.8%, and difference reaches the level of signifiance, illustrates that the rice shoot bud differentiation that the present invention is cultivated is good.
The field Streak Disease incidence of disease is 0 after the rice shoot field planting that the present invention is cultivated, and conventional hole plate seedling growth is cultivated
Rice shoot field planting after the field Streak Disease incidence of disease be 10.3%, difference reaches the level of signifiance, illustrate the of the invention rice shoot cultivated
Viral diseases ability is strong.
The field phytophthora root rot incidence of disease is 0 after the rice shoot field planting that the present invention is cultivated, and conventional hole plate seedling growth is cultivated
Rice shoot plant after the field phytophthora root rot incidence of disease be 4.1%, illustrate the of the invention rice shoot resistant to phytophthora root rot ability cultivated
By force.
The rice shoot disease resistance that the present invention is cultivated is strong, and Field diseases are low after field planting, and chemical pesticide can be reduced in production to be made
With 5 times, chemical pesticide usage amount reduces more than 40%.
The rice shoot 667m that the present invention is cultivated2Yield is 12258.3kg, the rice shoot 667m that conventional hole plate seedling growth is cultivated2Production
Measure as 9962.9kg, more conventional hole plate seedling growth of the invention improves 23.0%, and difference reaches the level of signifiance.
Embodiment 2, heliogreenhouse gets over summer stubble cultivation color green pepper, and varieties of plant is that century is red, and sowing on April 20, June 2 is fixed
Plant, place is in Shandong Shouguang green house of vegetables.If 2 processing, from 72 hole standard hole plate seedling growths, in addition to desired measure,
Other control measures are identical.
Cordceps militaris bacterium fermentate rich in scarlet caterpiller fungus mycelia, is prepared from by following steps：
1）The particle for the diameter 3-5mm sizes that corn kernel is ground into, is made corn kernel shot-like particle；
2）With corn kernel shot-like particle and wheat bran by volume 1：1 composition fermentation substrate；
3）After the activated culture of slant preservation strain, access by potato, glucose, skimmed milk power in mass ratio
1000：20：In the 2 semi-synthetic fluid nutrient mediums mixed, 20-28 DEG C is cultivated 10 days, produces Cordceps militaris liquid spawn；
4）By fermentation substrate and water according to 5：Load round after 1 mass ratio mixing, 115-132 DEG C sterilizes 20 points
Clock, after sterilizing terminates, is cooled to normal temperature under cleaning condition, standby；
5）Cordceps militaris liquid spawn is accessed by the 10%-15% of fermentation substrate quality, under the conditions of 20-28 DEG C, lucifuge training
After supporting 8 days, then culture 18 days under 100-150lx illumination condition are transferred to, that is, obtain the Cordceps militaris bacterium rich in scarlet caterpiller fungus mycelia
Fermentate.
Processing 1：Using the inventive method nursery
By Cordceps militaris bacterium fermentate, turf, vermiculite and perlite by volume 1：5：2：2 uniform mixing, while addition is suitable
Measure full dissolubility all-element compound fertilizer and prepare the seedling medium rich in scarlet caterpiller fungus mycelia, make seedling medium initial nutrient N, P2O5、
K2O content is respectively：50-60mg·kg-1、20-40mg·kg-1、80-90mg·kg-1, middle micro- appropriate, EC values 1.0-
1.5ms·cm-1, color green pepper hole plate seedling growth is carried out after medium in tray；Every plant is poured and applies concentration when after planting seed germination to cotyledon deploys
For 30mgkg-1Potassium phosphite aqueous solution 5-8ml；1st lobus cardiacus dew point pours to every plant during 11 heart of leaf and applies concentration for 20mg
kg-1Prohexadione calcium aqueous solution 8-10ml；Every plant is poured and applies concentration for 100mgkg during 21 heart of leaf-1Toxic fluoride phosphate aqueous solution 12-
15ml；Every plant is poured and applies concentration for 75mgkg during 31 heart of leaf-1Potassium phosphite aqueous solution 12-15ml.
Processing 2：Conventional method nursery
By 6: 2: the 2 uniform mixing by volume of turf, vermiculite, perlite, while the full elements compounding of the appropriate full dissolubility of addition
Fertilizer prepares seedling medium, makes seedling medium initial nutrient N, P2O5、K2O content is respectively：50-60mg·kg-1、20-40mg·
kg-1、80-90mg·kg-1, middle micro- appropriate, EC values 1.0-1.5mscm-1, color green pepper hole tray is carried out after medium in tray and is educated
Seedling, according to a conventional method spray on plants hormone control rice shoot excessive growth.
It is colonized during above-mentioned each processing 41 heart of leaf of rice shoot, root activity and seedling vigorous index is determined before field planting；Investigated after field planting color
The malformed fruit incidence of green pepper fruit, analyzes rice shoot bud differentiation situation；Investigating field diseases, a situation arises, analyzes the disease-resistant of rice shoot
Property；Count color green pepper total output.
Through experiment, the rice shoot seedling vigorous index that the present invention is cultivated is 0.0325, the rice shoot strong sprout that conventional hole plate seedling growth is cultivated
Index is 0.0264, and more conventional hole plate seedling growth of the invention improves 23.1%, and difference reaches the level of signifiance.
The rice shoot root activity that the present invention is cultivated is 82.7mgg-1·h-1, the rice shoot root system that conventional hole plate seedling growth is cultivated
Vigor is 68.1mgg-1·h-1, more conventional hole plate seedling growth of the invention improves 21.4%, significant difference.
The rice shoot malformed fruit rate that the present invention is cultivated is 0, and the rice shoot malformed fruit rate that conventional hole plate seedling growth is cultivated is
13.8%, difference reaches the level of signifiance, illustrates that the rice shoot bud differentiation that the present invention is cultivated is good.
The field Streak Disease incidence of disease is 0 after the rice shoot field planting that the present invention is cultivated, and conventional hole plate seedling growth is cultivated
Rice shoot field planting after the field Streak Disease incidence of disease be 13.9%, difference reaches the level of signifiance, illustrate the of the invention rice shoot cultivated
Viral diseases ability is strong.
The field phytophthora root rot incidence of disease is 0 after the rice shoot field planting that the present invention is cultivated, and conventional hole plate seedling growth is cultivated
Rice shoot plant after the field phytophthora root rot incidence of disease be 5.3%, illustrate the of the invention rice shoot resistant to phytophthora root rot ability cultivated
By force.
The field bacterial leaf spot incidence of disease is 2.5% after the rice shoot field planting that the present invention is cultivated, and conventional hole plate seedling growth training
The field bacterial leaf spot incidence of disease is 14.3% after the rice shoot field planting brought out, and difference reaches the level of signifiance, illustrates that the present invention is cultivated
Rice shoot bacteria resistance leaf spot ability it is strong.
The rice shoot disease resistance that the present invention is cultivated is strong, and Field diseases are low after field planting, and chemical pesticide can be reduced in production to be made
With 4 times, chemical pesticide usage amount reduces more than 40%.
The rice shoot 667m that the present invention is cultivated2Yield is 4926.8kg, the rice shoot 667m that conventional hole plate seedling growth is cultivated2Production
Measure as 4012.3kg, more conventional hole plate seedling growth of the invention improves 22.8%, and difference reaches the level of signifiance.
Embodiment 3, heliogreenhouse early spring stubble cultivation color green pepper, varieties of plant is tangerine West Asia, sowing on December 5, in January, the 2nd
It is colonized within 20th, place is in Shandong Shouguang green house of vegetables.If 2 processing, from 72 hole standard hole trays, except desired measure
Outside, other control measures are identical.
Cordceps militaris bacterium fermentate rich in scarlet caterpiller fungus mycelia, is prepared from by following steps：
1）The particle for the diameter 3-5mm sizes that corn kernel is ground into, is made corn kernel shot-like particle；
2）With corn kernel shot-like particle and wheat bran by volume 1：1 composition fermentation substrate；
3）After the activated culture of slant preservation strain, access by potato, glucose, skimmed milk power in mass ratio
1000：20：In the 2 semi-synthetic fluid nutrient mediums mixed, 20-28 DEG C is cultivated 10 days, produces Cordceps militaris liquid spawn；
4）By fermentation substrate and water according to 5：Load round after 1 mass ratio mixing, 115-132 DEG C sterilizes 20 points
Clock, after sterilizing terminates, is cooled to normal temperature under cleaning condition, standby；
5）Cordceps militaris liquid spawn is accessed by the 10%-15% of fermentation substrate quality, under the conditions of 20-28 DEG C, lucifuge training
After supporting 8 days, then culture 18 days under 100-150lx illumination condition are transferred to, that is, obtain the Cordceps militaris bacterium rich in scarlet caterpiller fungus mycelia
Fermentate.
Processing 1：Using the inventive method nursery
It will be enriched in Cordceps militaris bacterium fermentate, turf, vermiculite and the perlite of scarlet caterpiller fungus mycelia by volume 1：5：2：2 is equal
Even mixing, while the appropriate full dissolubility all-element compound fertilizer of addition prepares seedling medium, makes seedling medium initial nutrient N, P2O5、K2O
Content is respectively：50-60mg·kg-1、20-40mg·kg-1、80-90mg·kg-1, middle micro- appropriate, EC values 1.0-
1.5ms·cm-1, color green pepper hole plate seedling growth is carried out after medium in tray；Every plant is poured and applies concentration when after planting seed germination to cotyledon deploys
For 30mgkg-1Potassium phosphite aqueous solution 5-8ml；1st lobus cardiacus dew point pours to every plant during 11 heart of leaf and applies concentration for 20mg
kg-1Prohexadione calcium aqueous solution 8-10ml；Every plant is poured and applies concentration for 100mgkg during 21 heart of leaf-1Toxic fluoride phosphate aqueous solution 12-
15ml；Every plant is poured and applies concentration for 75mgkg during 31 heart of leaf-1Potassium phosphite aqueous solution 12-15ml.
Processing 2：Conventional method nursery
By 6: 2: the 2 uniform mixing by volume of turf, vermiculite, perlite, while the full elements compounding of the appropriate full dissolubility of addition
Fertilizer prepares seedling medium, makes seedling medium initial nutrient N, P2O5、K2O content is respectively：50-60mg·kg-1、20-40mg·
kg-1、80-90mg·kg-1, middle micro- appropriate, EC values 1.0-1.5mscm-1, color green pepper hole tray is carried out after medium in tray and is educated
Seedling, according to a conventional method spray on plants hormone control rice shoot excessive growth.
It is colonized during above-mentioned each processing 41 heart of leaf of rice shoot, root activity and seedling vigorous index is determined before field planting；Investigated after field planting color
The malformed fruit incidence of green pepper fruit, analyzes rice shoot bud differentiation situation；Investigating field diseases, a situation arises, analyzes the disease-resistant of rice shoot
Property；Count color green pepper total output.
Through experiment, the rice shoot seedling vigorous index that the present invention is cultivated is 0.0298, the rice shoot strong sprout that conventional hole plate seedling growth is cultivated
Index is 0.0241, and more conventional hole plate seedling growth of the invention improves 23.7%, and difference reaches the level of signifiance.
The rice shoot root activity that the present invention is cultivated is 86.9mgg-1·h-1, the rice shoot root system that conventional hole plate seedling growth is cultivated
Vigor is 71.0mgg-1·h-1, more conventional hole plate seedling growth of the invention improves 22.4%, significant difference.
The rice shoot malformed fruit rate that the present invention is cultivated is 0, and the rice shoot malformed fruit rate that conventional hole plate seedling growth is cultivated is
14.6%, difference reaches the level of signifiance, illustrates that the rice shoot bud differentiation that the present invention is cultivated is good.
The field mosaic virus incidence of disease is 0 after the rice shoot field planting that the present invention is cultivated, and conventional hole plate seedling growth is cultivated
Rice shoot field planting after the field mosaic virus incidence of disease be 11.3%, difference reaches the level of signifiance, illustrate the of the invention rice shoot cultivated
Viral diseases ability is strong.
The field phytophthora root rot incidence of disease is 0 after the rice shoot field planting that the present invention is cultivated, and conventional hole plate seedling growth is cultivated
Rice shoot plant after the field phytophthora root rot incidence of disease be 6.2%, illustrate the of the invention rice shoot resistant to phytophthora root rot ability cultivated
By force.
The field epidemic disease incidence of disease is 4.3% after the rice shoot field planting that the present invention is cultivated, and the seedling that conventional hole plate seedling growth is cultivated
The field epidemic disease incidence of disease is 12.9% after seedling field planting, and difference reaches the level of signifiance, illustrates the anti-epidemic disease ability of rice shoot that the present invention is cultivated
By force.
The field bacterial leaf spot incidence of disease is 2.5% after the rice shoot field planting that the present invention is cultivated, and conventional hole plate seedling growth training
The field bacterial leaf spot incidence of disease is 16.3% after the rice shoot field planting brought out, and difference reaches the level of signifiance, illustrates that the present invention is cultivated
Rice shoot bacteria resistance leaf spot ability it is strong.
The rice shoot disease resistance that the present invention is cultivated is strong, and Field diseases are low after field planting, and chemical pesticide can be reduced in production to be made
With 3 times, chemical pesticide usage amount reduces more than 40%.
The rice shoot 667m that the present invention is cultivated2Yield is 6738.4kg, the rice shoot 667m that conventional hole plate seedling growth is cultivated2Production
Measure as 5481.5kg, more conventional hole plate seedling growth of the invention improves 22.9%, and difference reaches the level of signifiance.
Citing described above for best mode for carrying out the invention, wherein the part do not addressed in detail is that this area is common
The common knowledge of technical staff.Protection scope of the present invention is defined by the content of claim, any technology based on the present invention
The equivalent transformation for enlightening and carrying out, also within protection scope of the present invention.
Claims (4)
1. the breeding method of the healthy seedling of a kind of color green pepper, it is characterised in that comprise the following steps：
1）Color green pepper seed is sowed at the 72 hole standard hole trays equipped with the seedling medium rich in scarlet caterpiller fungus mycelia, seed germination to cotyledon
Every plant is poured and applies concentration for 30mgkg during expansion-1Potassium phosphite aqueous solution 5-8ml；
2）1st lobus cardiacus dew point pours to every plant during 11 heart of leaf and applies concentration for 20mgkg-1Prohexadione calcium aqueous solution 8-10ml；
3）Every plant is poured and applies concentration for 100mgkg during 21 heart of leaf-1Toxic fluoride phosphate aqueous solution 12-15ml；
4）Every plant is poured and applies concentration for 75mgkg during 31 heart of leaf-1Potassium phosphite aqueous solution 12-15ml；
The healthy seedling is cultivated for color green pepper, and malformed fruit rate is 0；
Virosis, the phytophthora root rot incidence of disease are 0；
Chemical pesticide usage amount reduces more than 40%.
2. the breeding method of the healthy seedling of color green pepper according to claim 1, it is characterised in that：It is described rich in scarlet caterpiller fungus mycelia
Seedling medium is by Cordceps militaris bacterium fermentate, turf, vermiculite and perlite by volume 1：5：2：2 uniform mixed preparings.
3. the breeding method of the healthy seedling of color green pepper according to claim 2, it is characterised in that：The Cordceps militaris bacterium fermentate, leads to
Following steps are crossed to be prepared from：
1）The particle for the diameter 3-5mm sizes that corn kernel is ground into, is made corn kernel shot-like particle；
2）With corn kernel shot-like particle and wheat bran by volume 1：1 composition fermentation substrate；
3）After the activated culture of slant preservation strain, access by potato, glucose, skimmed milk power in mass ratio 1000：20：
In the 2 semi-synthetic fluid nutrient mediums mixed, 20-28 DEG C is cultivated 10 days, produces Cordceps militaris liquid spawn；
4）By fermentation substrate and water according to 5：Load round after 1 mass ratio mixing, 115-132 DEG C sterilizes 20 minutes, goes out
After bacterium is terminated, normal temperature is cooled under cleaning condition, it is standby；
5）Cordceps militaris liquid spawn, under the conditions of 20-28 DEG C, lucifuge culture 8 days are accessed by the 10%-15% of fermentation substrate quality
Afterwards, then be transferred under 100-150lx illumination condition cultivate 18 days, that is, Cordceps militaris bacterium fermentate is obtained, rich in scarlet caterpiller fungus mycelia.
4. the breeding method of the healthy seedling of color green pepper according to claim 1, it is characterised in that：It is described rich in scarlet caterpiller fungus mycelia
The appropriate full dissolubility all-element compound fertilizer of seedling medium addition, makes seedling medium initial nutrient N, P2O5、K2O content is respectively：
50-60mg·kg-1、20-40mg·kg-1、80-90mg·kg-1, middle micro- appropriate, EC values 1.0-1.5mscm-1。
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| CN107750850A (en) * | 2015-08-28 | 2018-03-06 | 寿光恒蔬无疆农业发展集团有限公司 | A kind of breeding method of color green pepper health seedling |
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| CN106472283A (en) * | 2016-10-20 | 2017-03-08 | 山东鑫诚现代农业科技有限责任公司 | A kind of glasshouse color green pepper soilless culture method |
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| JP2006191848A (en) * | 2005-01-13 | 2006-07-27 | Ajinomoto Co Inc | New method for cultivating fruit vegetable crop in undernutrition condition |
| CN102150531A (en) * | 2010-12-09 | 2011-08-17 | 苏州硒谷科技有限公司 | Hot pepper rich in organic selenium and production method thereof |
| CN102503739B (en) * | 2011-11-04 | 2014-04-02 | 广西田园生化股份有限公司 | Compound fertilizer of toxic fluorine-phosphorus and foliage fertilizer |
| CN102487708A (en) * | 2011-12-16 | 2012-06-13 | 珠海银基农产品有限公司 | Method for growing pepper |
| CN103766095A (en) * | 2012-10-18 | 2014-05-07 | 吴燕 | Pepper planting method |
| CN103392575B (en) * | 2013-08-06 | 2015-11-18 | 山东省寿光蔬菜产业集团有限公司 | A kind of Multifunctional seedling culturing matrix |
| CN103460937A (en) * | 2013-09-12 | 2013-12-25 | 江苏希旺农业科技有限公司 | Color pepper cultivation technology |
| CN104082025B (en) * | 2014-07-24 | 2016-03-23 | 寿光市新世纪种苗有限公司 | A kind of method of cultivating the disease-resistant seedling of color green pepper |
| CN104094764B (en) * | 2014-07-24 | 2016-03-23 | 寿光市新世纪种苗有限公司 | The breeding method of the disease-resistant seedling of a kind of color green pepper |
| CN104115672B (en) * | 2014-07-24 | 2016-05-04 | 山东百兴福农业发展股份有限公司 | The cultural method of a kind of tomato Chinese caterpillar fungus fruit |
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| CN107750850A (en) * | 2015-08-28 | 2018-03-06 | 寿光恒蔬无疆农业发展集团有限公司 | A kind of breeding method of color green pepper health seedling |
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Denomination of invention: A Method for Cultivating Healthy Seedlings of Colored Pepper Effective date of registration: 20230711 Granted publication date: 20170922 Pledgee: Shandong Shouguang Rural Commercial Bank Co.,Ltd. Pledgor: SHOUGUANG HENGSHU WUJIANG AGRICULTURAL DEVELOPMENT GROUP CO.,LTD. Registration number: Y2023980047941 |