CN104067841B - A kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method - Google Patents
A kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method Download PDFInfo
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Abstract
The invention discloses a kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method, cultivate including maternal plant, cutting strunk rush is sprouted, cutting selects and cuttage, maternal plant management and cutting seedling management operation, it is characterized in that: superior clone seedling growth is cultivated in equipped with the container of substrate mother plant for cutting, block Seedling dry doubling in maternal plant base portion and spray hormone promotion rudiment, select high-quality tender fringe bar through root-inducing promoter process after cuttage in cool canopy, spray mixotrophism liquid, it is thus achieved that Aquilaria sinensis (Lour.) Spreng. superior clone cutting seedling.This method can keep the merit of select tree completely, can effectively control the growth potential of maternal plant branch, the branch turning out high-quality makees cutting, April is to 11 monthly energy cuttages, preferably improve breeding coefficient and cuttage survival rate, meanwhile, with low cost, promote easily, there is provided a kind of method effective, reliable for Aquilaria sinensis (Lour.) Spreng. Germ-plasma resources protection and superior clone nursery stock popularization and afforestation, there is preferable Social benefit and economic benefit.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, relate to a kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method.
Background technology
Aquilaria sinensis (Lour.) Spreng. (Aquilaria sinensis (Lour.) Gilg) is thymelaeceae eaglewood, is that China is distinctive
Precious medicinal plants, is now the most sporadicly distributed in the provinces and regions such as Hainan, Guangdong, Guangxi, Fujian and Taiwan.Due to Aquilaria sinensis (Lour.) Spreng. the most more
New ability is weak, adds artificial indiscriminate felling of trees etc., causes wild resource the deficientest, is listed in national three-level protective in imminent danger and plants
Thing, two grades of emphasis wild protection plants of country.Within after Aquilaria sinensis (Lour.) Spreng. seed maturity under natural conditions about 10 days, i.e. losing vitality, pole is not
Storage tolerance, if not having cryopreservation condition in Chang Tuyunshu, percentage of seedgermination will drastically decline, and seriously constrain Aquilaria sinensis (Lour.) Spreng. nursery stock
Produce and the development of artificial forest.
Pass through asexual propagation, it is possible to solve the problem that seed is not enough and genetic variation and genetic differentiation is big, for Aquilaria sinensis (Lour.) Spreng. resource conservation with open
Send out to utilize and open up new approach.Had been achieved with successfully by the method propagation ground Lignum Aquilariae Resinatum of tissue culture but tissue culture's production bar
Part and technology require higher, and production cost is high, the most not yet can spread, and Technique on Cuttage Propagation is easily mastered, raw
Product condition requires low, and general nursery just can produce.At home, there is Liang Ju about Aquilaria sinensis (Lour.) Spreng. Technique on Cuttage Propagation research report
Red grade (Lignum Aquilariae Resinatum improved seeds non-line-of-sight error technical research, Tropical Forest, 2007,35 (2): 24-25), it is with 1 year raw Seedling
Divide three segment cuttages and juvenile form bud cuttage；Niu Huanqiong etc. (Aquilaria sinensis (Lour.) Spreng. asexual propagation Preliminary Report on Experiment, forest inventory and planning, 2010,35
(6) it is: 119-123.) to gather branch cutting from stump base portion；(different plant growth regulators are to Lignum Aquilariae Resinatum cuttage for Zhang Yuchen etc.
The impact taken root, forestry scientific research, 2010,23(2): be 278-282.) with raw seedling semi-lignified branch cuttage in 1 year,
It is more general that the cultivation of maternal plant and the system of selection etc. of management, cutting are introduced by these articles.About Aquilaria sinensis (Lour.) Spreng. cottage propagation
Patent has " cuttage and breeding method of Aquilaria sinensis " (application number: 200810100568.5), be with any branch all can cuttage, cuttage base
Matter is based on lichen, but the method rooting rate extremely unstable in practical operation, and high cost；" a kind of Aquilaria sinensis (Lour.) Spreng. half wood
The method of matter epicormic branch cutting " (application number: 201010262969), it is to make maternal plant with seed seedling-raising, uses semi-lignified
Branch makees cutting, and maternal plant belongs to seedling, it is impossible to ensure to keep completely the fine quality of elite stand, and not about adopting after fringe maternal plant
Management technique, it will the impact utilization to follow-up branch.The present invention is to use after select superior tree, enters with the tissue of select tree
The superior clone nursery stock of row tissue culture gained makees mother plant for cutting, is planted in container, can keep the excellent product of elite stand completely
Matter, controls maternal plant growth potential as required, the most all can carry out cottage propagation, and breeding coefficient is high, and cuttage survival rate is higher.
Summary of the invention
It is an object of the invention to the deficiency for current Aquilaria sinensis (Lour.) Spreng. vegetative propagation technique, it is provided that a kind of with low cost, popularization
Easily, the Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method that breeding coefficient is high, merit is stable, survival rate is high.
The purpose of the present invention is achieved through the following technical solutions:
A kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method, including maternal plant cultivation, cutting strunk rush is sprouted, cutting selects and cuttage, maternal plant
Management and cutting seedling manage operation, it is characterised in that: superior clone seedling growth is cultivated in equipped with the container of substrate and adopts
Fringe maternal plant, blocks Seedling dry doubling in maternal plant base portion and sprays hormone promotion rudiment, select high-quality tender fringe bar after root-inducing promoter processes
Cuttage, in cool canopy, sprays mixotrophism liquid, it is thus achieved that Aquilaria sinensis (Lour.) Spreng. superior clone cutting seedling, comprises the following steps:
(1) maternal plant is cultivated: is cultivated in equipped with the maternal plant cultivating container of maternal plant substrate by superior clone seedling growth and adopts fringe
Maternal plant；
(2) cutting strunk promotees to sprout: blocks trunk in maternal plant base portion, and topdresses and spray hormone promotion rudiment；
(3) cutting selects and cuttage: gathers high-quality tender fringe bar in April～November, processes with root-inducing promoter according to a conventional method
After cutting, by cutting cuttage to cool canopy built with in the container of cutting medium；
(4) maternal plant management: after gathering fringe bar, carry out applying fertilizer or cutting lateral root depending on maternal plant growing state；Described place of being completely cured
Reason is when maternal plant root system penetrates that maternal plant cultivating container is more, shoot growth is crossed prosperous, moves and cuts off bottom cultivating container, and flat cultivating container
Stretch out the maternal plant root outside cultivating container；
(5) cutting seedling management: use covered rearing with plastic film cutting bed, raised thin film every 5～7 days, depending on substrate dry and wet situation
Water according to a conventional method and spray mixotrophism liquid, after cuttage rooting is stable, removing thin film.
Above step (1) maternal plant cultivate described in its component of maternal plant substrate and parts by weight be: yellow soil or forest table
10 parts of soil, wood bran or coconut palm bran 10 parts, ripening farm manure or peat soil 0.1～0.3 part.
Above step (1) maternal plant cultivate described in maternal plant cultivating container be use back cut diameter be 15～20 cm, a height of 15
～20 containers of cm.
It is to be 25～30 cm by maternal plant cultivating container by spacing in the rows × line-spacing that maternal plant described in above step (1) cultivates its method
× 30～40 cm are placed on the non-woven fabrics that thickness is 0.1～0.2 cm.
Above step (2) cutting strunk promote sprout described in its method of trunk of blocking be the mother that footpath reaches 0.5～1.0 cm over the ground
Strain, blocks trunk at base portion 20～25 cm.
Above step (2) cutting strunk promote to sprout described in topdress and spray its method of hormone and be: female to every strain according to a conventional method
Strain imposes Beijing Huimin and reaches refined organic fertilizer 5 g+compound fertilizer (N:P:K=15:15:15) 5 g that development in science and technology center produces,
Water permeable, and spray the 6-Furfurylaminopurine (6KT) that mass concentration is 20～50 mg.L-1 according to a conventional method.
Above step (3) cutting selects and the high-quality fringe bar described in cuttage is to select epidermis in the range of base portion 1～3 cm
Gray, a length of 12～25 twig row culture cutting of cm；The shoot of length ＞ 20 cm, cuts off terminal bud.
Above step (3) cutting selects: yellow soil 70 parts,
Coconut palm bran 29 parts, Vermiculitum 1 part.
Its method of topdressing described in the management of above step (4) maternal plant is: after gathering fringe bar, according to a conventional method to often every time
Strain maternal plant is drenched and executes 0.5～1.0 % urea water liquid；After having gathered cutting November, every strain maternal plant then imposes Beijing Huimin and reaches science and technology
Refined organic fertilizer 5 g+compound fertilizer (N:P:K=15:15:15) 5 g that centre of development produces.
The mixotrophism liquid that sprays described in the management of above step (5) cutting seedling is by being 0.2～0.3 containing mass concentration
The mixotrophism liquid of % peptide urea+0.2～0.3 % ferrous sulfate composition.
The present invention has the advantage that relative to existing technology
1. this Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method, uses after select superior tree, carries out tissue training with the tissue of select tree
The superior clone nursery stock supporting gained makees cuttage maternal plant, it is possible to keeping the merit of select tree completely, the cutting seedling cultivated is
Superior clone Seedling, and seedling cost is well below tissue culture, can mainly educating as superior clone nursery stock popularization and afforestation
Seedling-growing method.
2. this Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method, use container plantation maternal plant, place mat non-woven fabrics or cinder under container, can
Effectively controlling the growth potential of maternal plant branch, the branch turning out high-quality is made cutting, is effectively improved cuttage survival rate.
3. this Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method, sprays 6-Furfurylaminopurine after maternal plant cutting strunk, can promote sprouting of bud
Send out, improve breeding coefficient；After cuttage, spray peptide urea+ferrous sulfate mixed solution, the photosynthetic of cutting can be effectively facilitated
Effect and the conversion of nutrient, thus promote cuttage rooting.
4, this Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method, with low cost, promote easily, it is Aquilaria sinensis (Lour.) Spreng. Germ-plasma resources protection and excellent
Good clone nursery stock popularization and afforestation provides a kind of method effective, reliable, has preferable Social benefit and economic benefit.
Detailed description of the invention
By specific examples presented below, the present invention can be well understood to further, but they are not to this
Bright restriction.
Embodiment 1
By superior clone seedling growth in back cut diameter be 20 cm, in the maternal plant cultivating container of a height of 18 cm, cultivating container
Interior substrate is yellow soil 10 parts, coconut palm bran 10 parts, peat soil 0.2 part.It is 25 × 40 cm by maternal plant cultivating container by spacing in the rows × line-spacing
It is placed on the non-woven fabrics that thickness is 0.1 cm.Footpath reaches the maternal plant of 0.6 cm and blocks trunk at base portion 25 cm, by often over the ground
Rule method imposes Beijing Huimin and reaches refined organic fertilizer 5 g+compound fertilizer (N:P:K=that development in science and technology center produces every strain maternal plant
15:15:15) 5 g, waters permeable, and to spray mass concentration according to a conventional method be 20 mg.L-16-Furfurylaminopurine (6KT).4
The moon selects epidermis gray, the twig row culture cutting of a length of 12 cm in the range of base portion 1 cm, according to a conventional method with promotion of taking root
Agent processes after cutting, by cutting cuttage to cool canopy built with in the container of cutting medium, and its component of cutting medium and parts by weight
For: yellow soil 70 parts, coconut palm bran 29 parts and Vermiculitum 1 part.Use covered rearing with plastic film cutting bed, raised thin film every 7 days, do depending on substrate
Wet situation is watered according to a conventional method and sprayed by mass concentration is the mixing that 0.2 % peptide urea+0.3 % ferrous sulfate forms
Nutritional solution, after cuttage rooting is stable, removes thin film.After gathering fringe bar, every strain maternal plant is drenched execute 0.5 % carbamide according to a conventional method
Water liquid, moves and cuts off the maternal plant root stretched out outside cultivating container bottom maternal plant cultivating container, and flat cultivating container.Every strain maternal plant averagely obtains cutting
Article 3, after cuttage about 35 days start to take root, cuttage survival rate 87.9 %.
Embodiment 2
By superior clone seedling growth in back cut diameter be 15 cm, in the maternal plant cultivating container of a height of 20 cm, cultivating container
In substrate be yellow soil 10 parts, wood bran 10 parts, through the farm manure 0.3 part of overcuring.By maternal plant cultivating container by spacing in the rows × line-spacing
It is that 30 × 30 cm are placed on the non-woven fabrics that thickness is 0.2 cm.Over the ground footpath reach the maternal plant of 0.8 cm at base portion 23 cm cut
Disconnected trunk, imposes Beijing Huimin according to a conventional method and reaches refined organic fertilizer 5 g that development in science and technology center produces+multiple every strain maternal plant
Hefei (N:P:K=15:15:15) 5 g, waters permeable, and to spray mass concentration according to a conventional method be 30 mg.L-16-bran amino
Purine (6KT).Select epidermis gray, the twig row culture cutting of a length of 20cm in the range of base portion 2 cm July, according to a conventional method
Process after cutting with root-inducing promoter, by cutting cuttage to cool canopy built with in the container of cutting medium, its component of cutting medium
With parts by weight it is: yellow soil 70 parts, coconut palm bran 29 parts, Vermiculitum 1 part.Use covered rearing with plastic film cutting bed, raised thin every 6 days
Film, regards substrate dry and wet situation and waters according to a conventional method and spray by mass concentration sub-as 0.3 % peptide urea+0.2 % sulphuric acid
The mixotrophism liquid of ferrum composition, after cuttage rooting is stable, removes thin film.After gathering fringe bar, according to a conventional method to every strain maternal plant
0.8 % urea water liquid is executed in pouring, moves and cuts off the maternal plant root stretched out outside cultivating container bottom maternal plant cultivating container, and flat cultivating container.Every strain
Maternal plant averagely obtains cutting 4, and after cuttage about 25 days start to take root, cuttage survival rate 92.3 %.
Embodiment 3
By superior clone seedling growth in back cut diameter be 20 cm, in the maternal plant cultivating container of a height of 20 cm, cultivating container
In substrate be 10 parts of table soil, wood bran 10 parts, through the farm manure 0.1 part of overcuring.By maternal plant cultivating container by spacing in the rows × line-spacing it is
30 × 40 cm are placed on the non-woven fabrics that thickness is 0.2 cm.Footpath reaches the maternal plant of 1 cm and blocks master at base portion 20 cm over the ground
Dry, according to a conventional method every strain maternal plant is imposed Beijing Huimin and reaches refined organic fertilizer 5 g+compound fertilizer that development in science and technology center produces
(N:P:K=15:15:15) 5 g, waters permeable, and to spray mass concentration according to a conventional method be 50 mg.L-16-Furfurylaminopurine
(6KT).Select epidermis gray, the twig row culture cutting of a length of 25 cm in the range of base portion 3 cm November, in distance base portion 20
Top is cut off, after processing cutting with root-inducing promoter according to a conventional method, by cutting cuttage to cool canopy built with cutting medium at cm
Container in, its component of cutting medium and parts by weight are: yellow soil 70 parts, coconut palm bran 29 parts, Vermiculitum 1 part.Cover with plastic sheeting
Lid cutting bed, raised thin film every 7 days, regards substrate dry and wet situation and waters according to a conventional method and spray by mass concentration as 0.3 %
The mixotrophism liquid of peptide urea+0.2 % ferrous sulfate composition, after cuttage rooting is stable, removes thin film.Gather fringe bar
After, every strain maternal plant then imposes Beijing Huimin and reaches refined organic fertilizer 5 g+compound fertilizer (N:P:K=that development in science and technology center produces
15:15:15) 5 g.Every strain maternal plant averagely obtains cutting 4, and after cuttage about 4 months start to take root, cuttage survival rate 90.7 %.
Claims (6)
1. an Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method, including maternal plant cultivation, cutting strunk rush is sprouted, cutting selects and cuttage, maternal plant pipe
Reason and cutting seedling manage operation, it is characterised in that: superior clone seedling growth is cultivated in equipped with the container of substrate and adopts fringe
Maternal plant, blocks Seedling dry doubling in maternal plant base portion and sprays hormone promotion rudiment, select high-quality tender fringe bar skewer after root-inducing promoter processes
Insert in cool canopy, spray mixotrophism liquid, it is thus achieved that Aquilaria sinensis (Lour.) Spreng. superior clone cutting seedling, comprise the following steps:
(1) maternal plant is cultivated: superior clone seedling growth is cultivated in equipped with the maternal plant cultivating container of maternal plant substrate mother plant for cutting；
(2) cutting strunk promotees to sprout: blocks trunk in maternal plant base portion, and topdresses and spray hormone promotion rudiment；
(3) cutting selects and cuttage: gathers high-quality tender fringe bar in April～November, processes cutting with root-inducing promoter according to a conventional method
After, by cutting cuttage to cool canopy built with in the container of cutting medium；
(4) maternal plant management: after gathering fringe bar, carry out applying fertilizer or cutting lateral root depending on maternal plant growing state；Described cutting lateral root is
When maternal plant root system penetrates that maternal plant cultivating container is more, shoot growth is crossed prosperous, move to cut off bottom cultivating container, and flat cultivating container and stretch out
Maternal plant root outside cultivating container；
(5) cutting seedling management: use covered rearing with plastic film cutting bed, raised thin film every 5～7 days, depending on substrate dry and wet situation by often
Rule method is watered and sprays mixotrophism liquid, after cuttage rooting is stable, removes thin film；
Step (1) maternal plant cultivate described in maternal plant matrix components and parts by weight be: yellow soil or forest top soil 10 parts, wood bran
Or coconut palm bran 10 parts, ripening farm manure or peat soil 0.1～0.3 part；
Step (2) cutting strunk promote sprout described in trunk method of blocking be the maternal plant that footpath reaches 0.5～1.0 cm over the ground, in base portion 20
～25 block trunk at cm；
Step (2) cutting strunk promote to sprout described in topdress and spray Hormonal methods and be: according to a conventional method every strain maternal plant is imposed Beijing
Huimin reaches compound fertilizer 5 g of the refined organic fertilizer 5 g+N:P:K=15:15:15 that development in science and technology center produces, and waters permeable, and presses
It is 20～50 mg.L that conventional method sprays mass concentration-16-Furfurylaminopurine；
Fertilizing method described in the management of step (4) maternal plant is: after gathering fringe bar every time, drenches every strain maternal plant according to a conventional method and executes
0.5～1.0 % urea water liquid；After having gathered cutting November, every strain maternal plant then imposes Beijing Huimin and reaches the life of development in science and technology center
Compound fertilizer 5 g of the refined organic fertilizer 5 g+N:P:K=15:15:15 produced.
2. a kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method described in a Ju claim 1, it is characterised in that: step (1) maternal plant is trained
Maternal plant cultivating container described in educating is that to use back cut diameter be 15～20 cm, a height of 15～20 containers of cm.
3. a kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method described in a Ju claim 1, it is characterised in that: described in step (1)
Maternal plant breeding method be maternal plant cultivating container is 25～30 cm × 30 by spacing in the rows × line-spacing～40 cm be placed in thickness be 0.1～
On the non-woven fabrics of 0.2 cm.
4. a kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method described in a Ju claim 1, it is characterised in that: step (3) cutting selects
Selecting fringe bar tender with the high-quality described in cuttage is to select epidermis gray in the range of base portion 1～3 cm, a length of 12～25 cm
Twig row culture cutting；The shoot of length ＞ 20 cm, cuts off terminal bud.
5. a kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method described in a Ju claim 1, it is characterised in that: step (3) cutting selects
Select and with the cutting medium component described in cuttage and parts by weight be: yellow soil 70 parts, coconut palm bran 29 parts, Vermiculitum 1 part.
6. a kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method described in a Ju claim 1, it is characterised in that: step (5) cutting seedling
Mixotrophism liquid described in management is by being that 0.2～0.3 % peptide urea+0.2～0.3 % sulphuric acid is sub-containing mass concentration
The mixotrophism liquid of ferrum composition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410335373.4A CN104067841B (en) | 2014-07-15 | 2014-07-15 | A kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410335373.4A CN104067841B (en) | 2014-07-15 | 2014-07-15 | A kind of Aquilaria sinensis (Lour.) Spreng. softwood cutting propagation method |
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| Publication Number | Publication Date |
|---|---|
| CN104067841A CN104067841A (en) | 2014-10-01 |
| CN104067841B true CN104067841B (en) | 2016-08-24 |
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| CN104996155B (en) * | 2015-08-08 | 2017-10-20 | 重庆天春科技有限公司 | The breeding method of agalloch eaglewood nursery stock |
| CN105493832A (en) * | 2015-12-09 | 2016-04-20 | 重庆三零三科技有限公司 | Daphone odora Var. Marginata cultivation method |
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| CN106034903B (en) * | 2016-05-31 | 2019-02-19 | 广西科技大学鹿山学院 | A kind of method of hill gooseberry's potted landscape cultivation |
| CN106211998A (en) * | 2016-07-29 | 2016-12-14 | 广西壮族自治区林业科学研究院 | The breeding method of Flos Lonicerae container cutting propagation Seedling |
| CN106386353A (en) * | 2016-09-14 | 2017-02-15 | 广西壮族自治区林业科学研究院 | Seedling growing method of tender branch cutting for keteleeria cyclolepis flous |
| CN107409925A (en) * | 2017-09-06 | 2017-12-01 | 广西壮族自治区林业科学研究院 | A kind of method of the asexual fast breeding of Moringa |
| CN110463501B (en) * | 2019-09-19 | 2022-03-29 | 云南省林业科学院 | Scion germination promoting method for cutting pinus khasys of 3 years old or older |
| CN114402821A (en) * | 2021-12-30 | 2022-04-29 | 海南万安农业开发有限公司 | Method for raising seedlings of semi-lignified twigs of agilawood by cutting |
| CN115104440B (en) * | 2022-06-30 | 2023-08-29 | 中国热带农业科学院热带生物技术研究所 | Seedling raising method for promoting cutting rooting of agilawood branches |
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