CN107251728A - A kind of extracting method of dendrobium candidum - Google Patents
A kind of extracting method of dendrobium candidum Download PDFInfo
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- CN107251728A CN107251728A CN201710322657.3A CN201710322657A CN107251728A CN 107251728 A CN107251728 A CN 107251728A CN 201710322657 A CN201710322657 A CN 201710322657A CN 107251728 A CN107251728 A CN 107251728A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The invention discloses a kind of extracting method of dendrobium candidum, the dendrobium candidum after pre-treatment is taken, water and citric acid after cleaning, after immersion, decoct, filter while hot, merging filtrate, film is depressurized under 70 80 DEG C of 0.06 0.08MPa of filtrate and is condensed into crushing after medicinal extract, extract dry, is produced.The present invention is improved the specific processing technology of ganoderma lucidum so that the active ingredient polysaccharide of ganoderma lucidum is easily measured, and the polyoses content measured is high so that the good drug efficacy of ganoderma lucidum.
Description
Technical field
The present invention relates to a kind of extracting method of dendrobium candidum, the extraction of the high dendrobium candidum of particularly a kind of polyoses content
Method.
Background technology
Dendrobium candidum nickname iron sheet orchid, bracketplant, Yunnan iron sheet, iron sheet bracketplant, are orchid family Dendrobium herbaceos perennials,
Dendrobium candidum is the top grade of Shihu " medicinal materials, have the laudatory title of " gold in Chinese celestial grass-medicine ", is used as medicine with stem, contains polysaccharide, stone
The active ingredients such as dry measure used in former times alkali, alkaloid, with nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, wet one's whistle improving eyesight the effects such as, it is antitumor, improve human body
In terms of immunity, treatment enterogastric diseases, anti-aging, anti-oxidant, platelet aggregation-against, reduction blood glucose, treatment cataract
There is good efficacy.
Modern pharmacology research shows that dendrobium candidum has humidification to non-specific and specific immunity, anti-swollen
In terms of the treatment of knurl, anti-oxidant, reduction blood glucose and cataract, pharmacological action is notable.Its chemical composition is more complicated, it has been determined that
Type of compounds mainly have：Alkaloids, polysaccharide, luxuriant and rich with fragrance class, bibenzyl parent nucleus class, wherein alkaloids, polysaccharide pharmacology are lived
Property is more notable.Polysaccharide constituents are the important substance basis that medicinal dendrobium plays immunocompetence effect, Dendrobium officinale polysaccharide energy
Peripheral leukocytes are significantly improved, promotes lymphocyte to produce migration inhibition factor, immunity of organisms can be effectively improved.2010 editions
《Pharmacopeia》The quality standard of dendrobium candidum has been recorded first, and assay is carried out to polysaccharide and mannose.
It is typically that dendrobium candidum is directly ground into powder, but invent when being processed in the prior art to dendrobium candidum
People has found in research process, when being processed using prior art to ganoderma lucidum, and the active ingredient polysaccharide of dendrobium candidum is difficult to survey
Go out so that the drug effect of ganoderma lucidum is preferable not enough.Simultaneously as the health-care efficacy of dendrobium candidum is widely recognized and connect by people
By dendrobium officinale far can not meet the demand of people.People have begun to use artificial cultivation dendrobium candidum, iron sheet
In the cultural method of the stem of noble dendrobium, culture medium is dendrobium candidum conditions on which persons or things depend for existence, different culture raw material and with can be to iron sheet
Growth, yield and active component of the stem of noble dendrobium etc. produce important influence.The culture medium of dendrobium candidum and existing in the prior art
Cultural method, is unfavorable for the quality guarantee of the dendrobium candidum of output.Particularly it cannot be guaranteed that in dendrobium candidum polysaccharide content, make
Polyoses content is low in the dendrobium candidum that must be finally given.
The content of the invention
The purpose of the present invention, is to provide a kind of extracting method of dendrobium candidum, specific processing technology of the present invention to ganoderma lucidum
Improved so that the active ingredient polysaccharide of ganoderma lucidum is easily measured, and the polyoses content measured is high so that the drug effect of ganoderma lucidum
It is good.
What the present invention was realized in.A kind of extracting method of dendrobium candidum, takes the dendrobium candidum after pre-treatment, after cleaning
With water and citric acid, after immersion, decoct, filter, merging filtrate, depressurized under 70-80 DEG C of 0.06-0.08MPa of filtrate thin while hot
Film is condensed into after medicinal extract, extract dry and crushed, and produces.
In the extracting method of foregoing dendrobium candidum, the dendrobium candidum after pre-treatment is taken, the 6-10 times of water measured is added after cleaning
With the 10-12 times of citric acid measured, soak after 1-3h, decoct 2-3 times, each 1-2h is filtered, merging filtrate while hot, filtrate 70-
Film is depressurized under 80 DEG C of 0.07MPa and is condensed into crushing after medicinal extract, extract dry, is produced.
The extracting method of foregoing dendrobium candidum, the pre-treatment is：Specifically include following steps：
(1) Seeds of Dendrobium Candidum is taken, after cleaning, 70-80% ethanol disinfection 5-15min is used on superclean bench
Afterwards, then with aseptic water washing 3-4 times, it is placed in after being blotted on the filter paper of sterilizing, is inoculated on germination medium, is 20- in daytime temperature
25 DEG C, under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；
(2) after after seed sprouting, the high single seedlings of 1-2cm are inoculated on proliferated culture medium, in 23-28 DEG C, light intensity 1500-
Daily illumination 9-10h under 2500lx, until dendrobium candidum height of seedling is 5-8cm；
(3) transplant, transplanting time is the 4-6 months, height of seedling is 5-8cm dendrobium candidum seedling, first cleans seedling root with clear water
Culture medium, then dilute immersion root with 0.3-0.5% potassium permanganate and sterilize, by line-spacing be 10-15cm dendrobium candidums seed in
In matrix, it is ensured that matrix covers root system, root system is set to be contacted with matrix；
(4) 1 bacterium ichor was sprayed every 3-5 days after planting, is continuously sprayed 20-30 days；Use sprays mode and carried out afterwards
Water, irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 3-6 months 1 time, 5-6 days 6-9 months 1 time, 2-3 days 1 9-12 months
It is secondary, March November to next year, harvesting, you can.
In the extracting method of foregoing dendrobium candidum, the step (1), the germination medium is main by by weight
10-20 parts of 5-15 parts of 3-6 parts of sucrose, the agar of calculation, 10-20 parts of the coptis and fritillaria are made as follows：The coptis and fritillaria add
The 10-12 times of decocting measured is boiled after 2-3h, filtering, takes filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, agar,
Produce.
The extracting method of foregoing dendrobium candidum, main 5 parts of sucrose, the fine jade by calculating by weight of the germination medium
15 parts of 10 parts of fat, 15 parts of the coptis and fritillaria are made as follows：The coptis and fritillaria add the decocting of 11 times of amounts to boil after 2.5h, mistake
Filter, takes filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, agar, produced.
In the extracting method of foregoing dendrobium candidum, the step (2), the proliferated culture medium is main by by weight
10-20 parts of 3-6 parts of the sucrose of calculation, 10-20 parts of sweet potato mash, 5-15 parts of soybean residue, NAA5-10 parts, 10-20 parts of Radix Angelicae Sinensis and Chinese ephedra are pressed
Following methods are made：Radix Angelicae Sinensis and Chinese ephedra add the 10-12 times of decocting measured to boil after 2-3h, filtering, take filter residue, dry, pulverize into thick
Grain, coarse granule is mixed with sucrose, sweet potato mash, soybean residue and NAA, is produced.
The extracting method of foregoing dendrobium candidum, main 5 parts of the sucrose, red by calculating by weight of the proliferated culture medium
15 parts of 15 parts of mashed potatoes, 10 parts of soybean residue, NAA8 parts, 15 parts of Radix Angelicae Sinensis and Chinese ephedra are made as follows：Radix Angelicae Sinensis and Chinese ephedra add 11 times
The decocting of amount is boiled after 2.5h, filtering, is taken filter residue, be dry, pulverize into coarse granule, coarse granule and sucrose, sweet potato mash, soybean residue and
NAA is mixed, and is produced.
In the extracting method of foregoing dendrobium candidum, the step (3), the main sugarcane by calculating by weight of the matrix
Sugared 3-6 parts, 10-20 parts of sweet potato mash, 10-20 parts of mashed potatoes, 5-15 parts of cornmeal mush, 5-15 parts of soybean residue, 10-20 parts of cultivated land, pellet
10-20 parts of 5-15 parts of skin, 10-20 parts of kuh-seng, 5-15 parts of the fruit of glossy privet and evodia rutaecarpa are made as follows：Cultivated land, the root bark of tree peony, hardship
Ginseng, the fruit of glossy privet and evodia rutaecarpa add the 10-12 times of decocting measured to boil after 2-3h, filter, take filter residue, dry, pulverize into coarse granule, thick
Grain is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, is produced.
The extracting method of foregoing dendrobium candidum, the matrix it is main by calculate by weight 5 parts of sucrose, sweet potato mash 15
Part, 15 parts of mashed potatoes, 10 parts of cornmeal mush, 10 parts of soybean residue, 15 parts of cultivated land, 10 parts of the root bark of tree peony, 15 parts of kuh-seng, 10 parts of the fruit of glossy privet and Wu
15 parts of the fruit of medicinal cornel is made as follows：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the 10-12 times of decocting measured to boil 2-3h
Afterwards, filter, take filter residue, dry, pulverize into coarse granule, coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue
It is even, produce.
Applicant has carried out substantial amounts of experimental study to the present invention, and part Experiment is as follows：
The polyoses content of experimental example 1 investigates
1 materials and methods
1.1 material
1.1.1 dendrobium candidum 1：Extracted by embodiment 1.
1.1.2 dendrobium candidum 2：Extracted by embodiment 1, be free of the coptis and fritillaria during pre-treatment in germination medium,
Be free of in proliferated culture medium and cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa are free of in Radix Angelicae Sinensis and Chinese ephedra, matrix, remaining method is same
Embodiment 1.
1.2 instruments and reagent
Dextrose standard sample [glucose of D mono- (+) one]：KAYON companies；Methanol (chromatographically pure)：Close europeanized of Tianjin section
Reagent Co., Ltd；Remaining reagent is that analysis is pure；Phenol：Shanghai Ling Feng chemical reagent Co., Ltd；Sulfuric acid：Insult peaking in Shanghai
Learn reagent Co., Ltd.
2 methods
The preparation of 2.1 need testing solutions
2.1.1 the preparation of need testing solution 1：
Dendrobium candidum of the present invention (dendrobium candidum 1) sample is taken, 50 DEG C is put in baking oven, dry, pulverize, No. three sieves is crossed, took
Medicinal powder after filter, volatilizes solvent, and add water 2000mL, is heated to reflux 2 hours, lets cool, is transferred in 250mL measuring bottles, with a small amount of
Moisture time washing container, washing lotion is incorporated in same measuring bottle, adds water to scale, shake up, and filters, and precision measures subsequent filtrate 2mL, put
In 15mL centrifuge tubes, precision adds 95% ethanol, shakes up, and refrigerates 1 hour, takes out, and centrifuges 20 minutes, abandoning supernatant, precipitation
Plus hot water dissolving, it is transferred in 25mL measuring bottles, lets cool, add water to scale, shake up, produces need testing solution 1.
2.1.2 the preparation of need testing solution 2
The sample of dendrobium candidum 2 is taken, 50 DEG C is put in baking oven, dry, pulverize, No. three sieves is crossed, takes medicinal powder after filtering, volatilize
Solvent, add water 2000mL, is heated to reflux 2 hours, lets cool, is transferred in 250mL measuring bottles, with a small amount of moisture time washing container, washes
Liquid is incorporated in same measuring bottle, adds water to scale, is shaken up, and filtration, precision measures subsequent filtrate 2mL, put in 15mL centrifuge tubes, and precision adds
Enter 95% ethanol, shake up, refrigerate 1 hour, take out, centrifuge 20 minutes, abandoning supernatant, precipitation plus hot water dissolving are transferred to
In 25mL measuring bottles, let cool, add water to scale, shake up, produce need testing solution 2.
The preparation of 2.2 reference substance solutions
It is appropriate to the DEXTROSE ANHYDROUS of constant weight that precision weighs 105 DEG C of dryings, adds water and 99mg/L solution is made, standby.
It is prepared by 2.3 standard curves
Precision measure reference substance solution 0.2,0.4,0.6,0.8,1.0m L put in 10m L tool plug test tubes, add water to
1.0mL, precision adds 5% phenol solution 1mL (I is now prepared before use), shakes up, then accurate addition sulfuric acid 5m L, shakes up, puts boiling
Heat 20 minutes, take out in water-bath, be put into ice-water bath and cool down 5 minutes, using corresponding reagent as blank, at 488nm wavelength
Absorbance is determined, using absorbency Y as ordinate, concentration X (g/L) is abscissa, draws standard curve, and regression equation is：Y=
11.772X-0.1492, r=0.9995.As a result show that polysaccharide linear relationship in the range of 0.02-0.10g/L is good.
2.4 determination method
Precision measures need testing solution 1 and each 0.5mL of need testing solution 2 respectively, puts in 10mL tool plug test tubes, adds water to
1mL, by the method under the preparation of " 2.3 " standard curve, from " precision adds 5% phenol solution 1m L ", measure is inhaled in accordance with the law
Luminosity, the content of DEXTROSE ANHYDROUS from need testing solution is read on standard curve, calculates, produces.
2.5 precision test
Precision draws 6 parts of glucose control product solution, and every part of 0.6m L determines absorbance according to the method under " 2.4 " item.
Measurement result, RSD is respectively 1.60% and 1.62%, and precision is good.
2.6 replica test
6 parts of need testing solutions 1 and need testing solution 2 are prepared as stated above, according to determining absorbance as stated above.Determine
As a result, RSD is respectively 2.70% and 2.71%, and repeatability is good.
2.7 stability test
Take same need testing solution 1 and need testing solution 2 respectively, place at room temperature, respectively at 10min, 30min, 1h,
Absorbance is determined after 2h, 4h, 6h as stated above.Measurement result, RSD is respectively 2.70% and 2.73%, shows that test sample is molten
Liquid 1 and need testing solution 2 are stable in 6 hours.
2.8 average recoveries are tested
6 parts of 1 powder of dendrobium candidum of known content (12.5%) is weighed, every part of about 1.65g is accurately weighed.It is accurate to add
Glucose control product solution (concentration is 0.099g/L) 2mL, prepares need testing solution 1, determines as stated above as stated above,
Polyoses content is calculated, as a result average recovery rate is that 94.9%, RSD is 2.7%.
6 parts of 2 powder of dendrobium candidum of known content (12.5%) is weighed, every part of about 1.65g is accurately weighed.It is accurate to add
Glucose control product solution (concentration is 0.099g/L) 2mL, prepares need testing solution 2, determines as stated above as stated above,
Polyoses content is calculated, as a result average recovery rate is that 93.9%, RSD is 2.6%.
2.9 samples are determined
By the preparation method and condition determination of above-mentioned need testing solution 1 and need testing solution 2,6 parts of samples of dendrobium candidum 1 are determined
The content of polysaccharide in product and 6 parts of samples of dendrobium candidum 2, records result, and results averaged the results are shown in Table 1.
The determination of polysaccharide result of table 1
As seen from table, the inventive method carry out extract obtained Dendrobium officinale polysaccharide content it is higher.The product of dendrobium candidum
Matter is good.
Compared with prior art, the present invention is improved the specific processing technology of ganoderma lucidum so that ganoderma lucidum it is effective into
Polysaccharide is divided easily to measure, and the polyoses content measured is high so that the good drug efficacy of ganoderma lucidum.
Embodiment
Embodiment 1.
A kind of extracting method of dendrobium candidum：The dendrobium candidum after pre-treatment is taken, 8 times of water measured and 11 times of amounts are added after cleaning
Citric acid, immersion 2h after, decoct 2 times, each 1.5h is filtered, merging filtrate while hot, is subtracted under 70-80 DEG C of 0.07MPa of filtrate
Ironed film is condensed into after medicinal extract, extract dry and crushed, and produces.
The pre-treatment is：Specifically include following steps：
(1) Seeds of Dendrobium Candidum is taken, after cleaning, after the ethanol disinfection 10min that 75% is used on superclean bench, then is used
Aseptic water washing 3 times, is placed in after being blotted on the filter paper of sterilizing, is inoculated on germination medium, is 20-25 DEG C, night temperature in daytime temperature
Under the conditions of 18-20 DEG C, light culture, until seed is sprouted；The germination medium by sucrose 5kg, agar 10kg, coptis 15kg and
Fritillaria 15kg is made as follows：The coptis and fritillaria add the decocting of 11 times of amounts to boil after 2.5h, filter, take filter residue, dry, pulverize
Into coarse granule, coarse granule is mixed with sucrose, agar, is produced；
(2) after after seed sprouting, the high single seedlings of 1-2cm are inoculated on proliferated culture medium, in 23-28 DEG C, light intensity 1500-
Daily illumination 8h under 2500lx, until dendrobium candidum height of seedling is 5-8cm；The proliferated culture medium is by sucrose 5kg, sweet potato mash
15kg, soybean residue 10kg, NAA8kg, Radix Angelicae Sinensis 15kg and Chinese ephedra 15kg are made as follows：Radix Angelicae Sinensis and Chinese ephedra add 11 times amount
Decocting is boiled after 2.5h, filtering, takes filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, sweet potato mash, soybean residue and NAA
It is even, produce；
(3) transplant, transplanting time is the 4-6 months, height of seedling is 5-8cm dendrobium candidum seedling, first cleans seedling root with clear water
Culture medium, then diluted immersion root with 0.4% potassium permanganate and sterilized, be 10-15cm dendrobium candidum seeds in matrix by line-spacing
In, it is ensured that matrix covers root system, root system is contacted with matrix；The matrix is by sucrose 5kg, sweet potato mash 15kg, mashed potatoes
15kg, cornmeal mush 10kg, soybean residue 10kg, cultivated land 15kg, root bark of tree peony 10kg, kuh-seng 15kg, fruit of glossy privet 10kg and evodia rutaecarpa 15kg
It is made as follows：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the decocting of 11 times of amounts to boil after 2.5h, filter, take filter
Slag, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, produced；
(4) 1 bacterium ichor was sprayed every 4 days after planting, is continuously sprayed 25 days；Use sprays mode and watered afterwards,
Irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 3-6 months 1 time, 5-6 days 6-9 months 1 time, 2-3 days 9-12 months 1 time, 11
The moon to March next year, harvesting, you can.
Embodiment 2.
A kind of extracting method of dendrobium candidum：The dendrobium candidum after pre-treatment is taken, 10 times of water measured and 12 times are added after cleaning
After the citric acid of amount, immersion 3h, decoct 3 times, each 2h is filtered, merging filtrate while hot, is subtracted under 70-80 DEG C of 0.07MPa of filtrate
Ironed film is condensed into after medicinal extract, extract dry and crushed, and produces.
The pre-treatment is：Specifically include following steps：
(1) Seeds of Dendrobium Candidum is taken, after cleaning, after the ethanol disinfection 15min that 80% is used on superclean bench, then is used
Aseptic water washing 4 times, is placed in after being blotted on the filter paper of sterilizing, is inoculated on germination medium, is 20-25 DEG C, night temperature in daytime temperature
Under the conditions of 18-20 DEG C, light culture, until seed is sprouted；The germination medium by sucrose 6kg, agar 15kg, coptis 20kg and
Fritillaria 20kg is made as follows：The coptis and fritillaria add the decocting of 12 times of amounts to boil after 3h, filter, take filter residue, dry, pulverize into
Coarse granule, coarse granule is mixed with sucrose, agar, is produced；
(2) after after seed sprouting, the high single seedlings of 1-2cm are inoculated on proliferated culture medium, in 23-28 DEG C, light intensity 1500-
Daily illumination 10h under 2500lx, until dendrobium candidum height of seedling is 5-8cm；The proliferated culture medium is by sucrose 6kg, sweet potato mash
20kg, soybean residue 15kg, NAA10kg, Radix Angelicae Sinensis 20kg and Chinese ephedra 20kg are made as follows：Radix Angelicae Sinensis and Chinese ephedra add 12 times amount
Decocting is boiled after 3h, filtering, takes filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, sweet potato mash, soybean residue and NAA,
Produce；
(3) transplant, transplanting time is the 4-6 months, height of seedling is 5-8cm dendrobium candidum seedling, first cleans seedling root with clear water
Culture medium, then diluted immersion root with 0.5% potassium permanganate and sterilized, be 10-15cm dendrobium candidum seeds in matrix by line-spacing
In, it is ensured that matrix covers root system, root system is contacted with matrix；The matrix is by sucrose 6kg, sweet potato mash 20kg, mashed potatoes
20kg, cornmeal mush 15kg, soybean residue 15kg, cultivated land 20kg, root bark of tree peony 15kg, kuh-seng 20kg, fruit of glossy privet 15kg and evodia rutaecarpa 20kg
It is made as follows：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the decocting of 12 times of amounts to boil after 3h, filter, take filter residue,
Coarse granule is dry, pulverize into, coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, produced；
(4) 1 bacterium ichor was sprayed every 3-5 days after planting, is continuously sprayed 30 days；Use sprays mode and poured afterwards
Water, irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 3-6 months 1 time, 5-6 days 6-9 months 1 time, 2-3 days 1 9-12 months
It is secondary, March November to next year, harvesting, you can.
Embodiment 3.
The dendrobium candidum after pre-treatment is taken, adds after cleaning after the water and 10 times of citric acids measured of 6 times of amounts, immersion 1h, decocts 2
Secondary, each 1h is filtered, merging filtrate while hot, and film is depressurized under 70-80 DEG C of 0.07MPa of filtrate and is condensed into medicinal extract, extract dry
After crush, produce.
The pre-treatment is：Specifically include following steps：
(1) Seeds of Dendrobium Candidum is taken, after cleaning, after the ethanol disinfection 5min that 70% is used on superclean bench, then is used
Aseptic water washing 3 times, is placed in after being blotted on the filter paper of sterilizing, is inoculated on germination medium, is 20-25 DEG C, night temperature in daytime temperature
Under the conditions of 18-20 DEG C, light culture, until seed is sprouted；The germination medium by sucrose 3kg, agar 5kg, coptis 10kg and
Fritillaria 10kg is made as follows：The coptis and fritillaria add the decocting of 10 times of amounts to boil after 2h, filter, take filter residue, dry, pulverize into
Coarse granule, coarse granule is mixed with sucrose, agar, is produced；
(2) after after seed sprouting, the high single seedlings of 1-2cm are inoculated on proliferated culture medium, in 23-28 DEG C, light intensity 1500-
Daily illumination 9h under 2500lx, until dendrobium candidum height of seedling is 5-8cm；The proliferated culture medium is by sucrose 3kg, sweet potato mash
10kg, soybean residue 5kg, NAA5kg, Radix Angelicae Sinensis 10kg and Chinese ephedra 10kg are made as follows：Radix Angelicae Sinensis and Chinese ephedra add the water of 10 times of amounts
Decoct after 2h, filtering takes filter residue, dry, pulverize into coarse granule, coarse granule is mixed with sucrose, sweet potato mash, soybean residue and NAA, i.e.,
；
(3) transplant, transplanting time is the 4-6 months, height of seedling is 5-8cm dendrobium candidum seedling, first cleans seedling root with clear water
Culture medium, then diluted immersion root with 0.3% potassium permanganate and sterilized, be 10-15cm dendrobium candidum seeds in matrix by line-spacing
In, it is ensured that matrix covers root system, root system is contacted with matrix；The matrix is by sucrose 3kg, sweet potato mash 10kg, mashed potatoes
10kg, cornmeal mush 5kg, soybean residue 5kg, cultivated land 10kg, root bark of tree peony 5kg, kuh-seng 10kg, fruit of glossy privet 5kg and evodia rutaecarpa 10kg are pressed
The method of stating is made：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the decocting of 10 times of amounts to boil after 2h, filter, take filter residue, do
It is dry, coarse granule is ground into, coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, produced；
(4) 1 bacterium ichor was sprayed every 3 days after planting, is continuously sprayed 20 days；Use sprays mode and watered afterwards,
Irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 3-6 months 1 time, 5-6 days 6-9 months 1 time, 2-3 days 9-12 months 1 time, 11
The moon to March next year, harvesting, you can.
Claims (9)
1. a kind of extracting method of dendrobium candidum, it is characterised in that：The dendrobium candidum after pre-treatment is taken, water and lemon are used after cleaning
Acid, after immersion, is decocted, filters while hot, merging filtrate, and film is depressurized under 70-80 DEG C of 0.06-0.08MPa of filtrate and is condensed into leaching
Crush, produce after cream, extract dry.
2. the extracting method of dendrobium candidum as claimed in claim 1, it is characterised in that：The dendrobium candidum after pre-treatment is taken, is washed
Add after net after the 6-10 times of water and the 10-12 times of citric acid measured measured, immersion 1-3h, decoct 2-3 times, each 1-2h is filtered while hot
Cross, merging filtrate, film is depressurized under 70-80 DEG C of 0.07 MPa of filtrate and is condensed into crushing after medicinal extract, extract dry, is produced.
3. the extracting method of dendrobium candidum as claimed in claim 1 or 2, it is characterised in that：The pre-treatment is：Specifically include
Following steps：
Take Seeds of Dendrobium Candidum, after cleaning, on superclean bench using 70-80% ethanol disinfection 5-15min after, then with nothing
Bacterium water is rinsed 3-4 times, is placed in after being blotted on the filter paper of sterilizing, is inoculated on germination medium, is 20-25 DEG C, night temperature in daytime temperature
Under the conditions of 18-20 DEG C, light culture, until seed is sprouted；
After after seed sprouting, the high single seedlings of 1-2cm are inoculated on proliferated culture medium, in 23-28 DEG C, light intensity 1500-2500lx
Daily illumination 9-10h down, until dendrobium candidum height of seedling is 5-8cm；
（3）Transplant, transplanting time is the 4-6 months, height of seedling is 5-8cm dendrobium candidum seedling, and the training of seedling root is first cleaned with clear water
Base is supported, then is sterilized with 0.3-0.5% potassium permanganate dilution immersion root, is 10-15cm dendrobium candidum seeds in matrix by line-spacing
In, it is ensured that matrix covers root system, root system is contacted with matrix；
（4）1 bacterium ichor was sprayed after plantation every 3-5 days, is continuously sprayed 20-30 days；Use sprays mode and watered afterwards,
Irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 3-6 months 1 time, 5-6 days 6-9 months 1 time, 2-3 days 9-12 months 1 time, 11
The moon to March next year, harvesting, you can.
4. the extracting method of dendrobium candidum as claimed in claim 2, it is characterised in that：The step（1）In, it is described to sprout training
Base is supported mainly by 10-20 parts of 5-15 parts of 3-6 parts of sucrose, agar calculating by weight, 10-20 parts of the coptis and fritillaria by following
Method is made：The coptis and fritillaria add the 10-12 times of decocting measured to boil after 2-3h, filtering, take filter residue, dry, pulverize into coarse granule, slightly
Particle is mixed with sucrose, agar, is produced.
5. the extracting method of dendrobium candidum as claimed in claim 3, it is characterised in that：The germination medium is main by by weight
15 parts of 5 parts of sucrose, 10 parts of agar, 15 parts of the coptis and the fritillaria that amount part calculates are made as follows：The coptis and fritillaria add 11 times
The decocting of amount is boiled after 2.5h, filtering, takes filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, agar, produced.
6. the extracting method of dendrobium candidum as claimed in claim 2, it is characterised in that：The step（2）In, the propagation training
Support base main by 3-6 parts of the sucrose calculated by weight, 10-20 parts of sweet potato mash, 5-15 parts of soybean residue, NAA5-10 parts, Radix Angelicae Sinensis
10-20 parts are made as follows with 10-20 parts of Chinese ephedra：Radix Angelicae Sinensis and Chinese ephedra add the 10-12 times of decocting measured to boil after 2-3h, filtering,
Filter residue is taken, coarse granule is dry, pulverize into, coarse granule is mixed with sucrose, sweet potato mash, soybean residue and NAA, produced.
7. the extracting method of dendrobium candidum as claimed in claim 5, it is characterised in that：The proliferated culture medium is main by by weight
5 parts of sucrose that amount part calculates, 15 parts of sweet potato mash, 10 parts of soybean residue, NAA8 parts, 15 parts of 15 parts of Radix Angelicae Sinensis and Chinese ephedra make as follows
Into：Radix Angelicae Sinensis and Chinese ephedra add the decocting of 11 times of amounts to boil after 2.5h, filter, take filter residue, dry, pulverize into coarse granule, coarse granule and sugarcane
Sugar, sweet potato mash, soybean residue and NAA are mixed, and are produced.
8. the extracting method of the dendrobium candidum as described in claim asks 2, it is characterised in that：The step（3）In, the matrix
Mainly by 3-6 parts of the sucrose calculated by weight, 10-20 parts of sweet potato mash, 10-20 parts of mashed potatoes, 5-15 parts of cornmeal mush, soybean residue
10-20 parts of 5-15 parts, 10-20 parts of cultivated land, 5-15 parts of the root bark of tree peony, 10-20 parts of kuh-seng, 5-15 parts of the fruit of glossy privet and evodia rutaecarpa press following sides
Method is made：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the 10-12 times of decocting measured to boil after 2-3h, filtering, take filter residue, do
It is dry, coarse granule is ground into, coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, produced.
9. the extracting method of the dendrobium candidum as described in claim asks 7, it is characterised in that：The matrix is main by by weight
5 parts of the sucrose of calculating, 15 parts of sweet potato mash, 15 parts of mashed potatoes, 10 parts of cornmeal mush, 10 parts of soybean residue, 15 parts of cultivated land, 10 parts of the root bark of tree peony,
15 parts of 15 parts of kuh-seng, 10 parts of the fruit of glossy privet and evodia rutaecarpa are made as follows：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add
The 10-12 times of decocting measured is boiled after 2-3h, filtering, takes filter residue, dry, pulverize into coarse granule, coarse granule and sucrose, sweet potato mash, soil
Beans mud, cornmeal mush and soybean residue are mixed, and are produced.
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