CN103947642A - Growth hormone type cane shoot fertilization promoting agent and spraying method thereof - Google Patents
Growth hormone type cane shoot fertilization promoting agent and spraying method thereof Download PDFInfo
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- CN103947642A CN103947642A CN201410147462.6A CN201410147462A CN103947642A CN 103947642 A CN103947642 A CN 103947642A CN 201410147462 A CN201410147462 A CN 201410147462A CN 103947642 A CN103947642 A CN 103947642A
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Abstract
The invention discloses a growth hormone type cane shoot fertilization promoting agent and a spraying method thereof. The cane shoot fertilization promoting agent consists of the following components: growth hormones, abscisic acid and gibberellin, wherein the weight ratio of the growth hormones to the abscisic acid to the gibberellin is 2:(1-2):1. A spraying method is characterized in that the first-time spraying is performed in a four to five-leaf stage during the growth of cane shoots, and the second-time spraying is performed with the same dosage one week later. Through reasonable proportion of the growth hormones IAA, ABA and GA3 and by adopting the reasonable spraying method with the spraying dosage at a proper time, the fertilization of the cane shoots can be promoted, the yield of the cane shoots can be increased, and the content of the IAA, the ABA and the GA3 has no remarkable difference with that of a contrast or is lower than that of the contrast, and therefore, the content abnormity of the hormones in a cane shoot body is not caused. In comparison with a cane shoot field which is managed generally or managed by virtue of a nutrient solution, for the cane shoot field managed by adopting the method disclosed by the invention, the quantity and the yield of the cane shoots in the field are remarkably improved, and economic benefits are obviously improved.
Description
Technical field
The invention belongs to production technology of crops field, be specifically related to a kind ofly promote the pregnant hay of wild rice stem, improve wild rice stem field output and do not cause the hay agent of growth hormone wild rice stem progestogenic and the spraying method that wild rice stem hormone in vivo content is abnormal.
Background technology
Wild rice stem (
zizania latifoliaturcz.) for grass family wild rice belongs to perennial root herbaceous plant, it is the important aquatic vegetable of one that originates in China, extensively cultivate in the Yangtze river basin and areas to the south, become at present the second largest aquatic vegetable of China except lotus rhizome, only in the cultivated area in Zhejiang Province just up to more than 50 ten thousand mu, there is important economic worth.Wild rice stem claims again wild rice, carving recklessly or melon, is one of ancient Chinese five cereals.Wild rice stem is nutritious, contain more protein, dietary fiber, potassium, phosphorus, sodium, magnesium, nickel, cobalt and thiamine, and moisture is lower, essential amino acids content is abundant, proportion of composing is reasonable, far exceedes other cereals and beans, and protein effect is higher than flour, rice and soybean.In addition, wild rice stem also has very high medical value, records according to medical book, and wild rice stem is hidden sweet cool in nature, has heat-clearing solution to be tired of, to promote the production of body fluid to quench thirst, to adjust the function such as stomach, sharp stool and urine.
The pregnant hay of wild rice stem and its endogenetic fungus-wild rice smut to infect interaction close.There are three kinds of phenotypes in wild rice stem field, i.e. normal wild rice stem (edible), grey hay (wild rice stem stem is full of wild rice smut spore) and male hay (stem does not expand) while production.Histologic section is observed and is shown: wild rice smut is expanded stem at normal wild rice stem and exists in a large number, and the vascular bundle tissue of this external wild rice stem subterranean stem, leaf sheath and bud and parenchyma are also observed the distribution of wild rice smut.Wild rice smut is infected after wild rice stem success, and wild rice stem is bloomed and is suppressed, and stimulates wild rice stem stem to expand, and forms the edible stem that expands.There is report to think that it is to cause due to the increase of wild rice stem stem growth hormone and Cytokinin Contents that wild rice stem stem expands.Research find, when wild rice stem fleshy stem expands, IAA content increasing degree is less, the duration is longer, before IAA content ratio in fleshy stem expands process expands rise, until expand the later stage just occur decline; Expanding of fleshy stem cell and gap thereof may be that IAA plays a major role; Wild rice stem expands GA in process
3lower with ABA content.In the wild rice smut zymotic fluid of cultured in vitro, plant hormone IAA, ABA and GA detected
3, wherein IAA content is 0.1306 mgL
-1, ABA content is 0.01367 mgL
-1.As can be seen here, the somatotropin relevant to the pregnant hay of wild rice stem may jointly be produced by wild rice stem plant and endogenetic fungus thereof in Interaction, very likely the pregnant hay of wild rice stem is exerted an influence with the factor of wild rice stem plant and wild rice smut growth correlation.
At present, for promoting the pregnant hay of wild rice stem, improve Water Bamboo Yield mainly by methods such as field rich water control, breed improvements.There is not yet the pregnant hay of similar promotion wild rice stem, improve the somatotropin series products of Water Bamboo Yield.
Summary of the invention
For the defect existing in prior art, the object of this invention is to provide a kind of Reasonable by different somatotropin and recently promote the pregnant hay of wild rice stem and output but do not cause the hay agent of growth hormone wild rice stem progestogenic and the spraying method that wild rice stem hormone in vivo content is abnormal.
Described growth hormone wild rice stem progestogenic hay agent, is characterized in that this wild rice stem progestogenic hay agent is composed of the following components: growth hormone, abscisic acid, gibberellin, three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:1-2:1.
Described growth hormone wild rice stem progestogenic hay agent, is characterized in that this wild rice stem progestogenic hay agent is composed of the following components, growth hormone, abscisic acid, gibberellin, and three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:2:1.
Described growth hormone wild rice stem progestogenic hay agent, is characterized in that this wild rice stem progestogenic hay agent is composed of the following components, growth hormone, abscisic acid, gibberellin, and three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:1:1.
The spraying method of described growth hormone wild rice stem progestogenic hay agent, is characterized in that the method is made up of following steps:
1) Jiao Dun selects: select hay Tanaka normally to tie hay and the similar Jiao Dun of proterties in field planting in the last ten-days period in March, conventional field management;
2) preparation of progestogenic hay dilution agent solution: take the somatotropin of formula ratio, dissolve with ethanol, it is 5% that ultra-pure water is settled to mother liquid concentration, when use 1000 times of mother liquor dilute with waters progestogenic hay dilution agent solution;
3) spray: in the 4-5 leaf phase of wild rice stem growth, spray on the blade and leaf sheath of wild rice stem plant with progestogenic hay dilution agent solution, sprayed once every 7 days, spray continuously twice.
The spraying method of described growth hormone wild rice stem progestogenic hay agent, is characterized in that step 2) in concentration of alcohol be 95%.
The spraying method of described growth hormone wild rice stem progestogenic hay agent, is characterized in that step 2) the w/v g:ml of the agent of progestogenic hay and ethanol is 4-5:2.
The spraying method of described growth hormone wild rice stem progestogenic hay agent, is characterized in that in step 3), and the amount of spraying is that every pier wild rice stem sprays 4L progestogenic hay dilution agent solution at every turn.
The present invention compared with prior art its advantage is: by different somatotropin IAA, ABA, GA
3rational proportion, in the suitable time, adopt after rational spraying method and the amount of spraying, can promote the pregnant hay of wild rice stem, increase Water Bamboo Yield, the hay field that adopts the hay field of the inventive method management to manage and adopt nutrient solution to manage than conventional wild rice stem, its knot hay number and knot hay output significantly improve, and wild rice stem body interior IAA, ABA and GA
3content does not have significance difference XOR lower than contrast with contrasting, and does not therefore cause that wild rice stem hormone in vivo content is abnormal, and its economic benefit obviously improves.
Embodiment
Further illustrate the present invention below in conjunction with specific embodiment.
1 field trial
1.1 test materials: tested in 2013 and carry out in experiment field, Dong family's wild rice stem base, Tongxiang City, Zhejiang Province, test material is No. two, imperial hay, select hay Tanaka in 2012 normally to tie hay and the similar Jiao Dun of proterties, be colonizated in plastic basin (80 × 60 × 50 cm March 25
3) in, every basin 1 pier Basic Seedling.Conventional field management.
1.2 experimental scheme: field trial is established four treatment combinations altogether, are respectively and process one, process two, process three, process four, 3 repetitions of each processing.
Process a formula: growth hormone: abscisic acid: gibberellin=2:2:1; Specifically be formulated as: take respectively in proportion 1gIAA, 1gABA and 0.5g GA
3, be dissolved in 1ml 95 ℅ ethanol, add ultra-pure water constant volume and make mother liquor to 50ml, 4 DEG C keep in Dark Place for subsequent use.While spraying, by 1000 times of uses of mother liquor dilution.
Process two formulas: growth hormone: abscisic acid: gibberellin=2:1:1; Specifically be formulated as: take respectively in proportion 1gIAA, 0.5gABA and 0.5g GA
3, be dissolved in 1ml 95 ℅ ethanol, add ultra-pure water constant volume and make mother liquor to 40ml, 4 DEG C keep in Dark Place for subsequent use.While spraying, by 1000 times of uses of mother liquor dilution.
Process three formulas: processing three is nutrient solution prescription: 500 times of ' trace element water-soluble fertilizer ' dilute with waters, be specially the trace element water-soluble fertilizer 50g 2.5kg that adds water, ' spray health ' 25g is dissolved in 15kg water, and equal-volume mixes rear use.Wherein ' trace element water-soluble fertilizer ' provided by Guilin flower letter plant tissue culture seedling Co., Ltd; ' spray health ' provided by Shanxi Kaisheng Fertilizer Industry Co., Ltd..
Process four for contrast CK, adopt clear water to spray.
Each processing respectively sprays alignment processing formula, and the amount of spraying is that every pier sprays 4L at every turn, all sprays twice, sprays for the first time in the 4-5 leaf phase of wild rice stem growth, and spraying for the first time the concrete time is on April 3rd, 2013, and the time that sprays is for the second time on April 10th, 2013.Different times in wild rice stem growth carries out field statistical observation to it.
1.3 sample collections: after spraying for the first time every getting sample 1 week one time, when sampling, dig out Jiao Dun from field plastic basin, after cleaning desilt, measure plant height, choose wild rice stem basal part of stem material, 3 repetitions of each processing, respectively with clean husky cloth pack, after mark, liquid nitrogen flash freezer is preserved, and takes back laboratory and put-70 DEG C of refrigerators for subsequent use.
1.4 hormone-contents are measured: sample extracts with 80% methyl alcohol homogenate, with Dot-ELISA (ELISA) mensuration heteroauxin (IAA), gibberellin (GA
3) and the content of abscisic acid (ABA), each sample is established 3 repetitions.
ELISA kit is purchased from Yong Hui bio tech ltd, Beijing.
Hormone extracting method: take 1g wild rice stem stem material, (80% methyl alcohol includes 1.0 mmolL to add 2mL sample extracting solution
-1toluene di-tert-butyl phenol), in ice bath, grind to form homogenate, proceed to 10mL test tube, with the gradation of 2mL extract, mortar is rinsed well again, proceeded in the lump in test tube, shake up to be placed in 4 DEG C of refrigerators and leave standstill after 4h, with the centrifugal 15min of 1000 × g, get supernatant and cross C-18 solid-phase extraction column, then the sample of crossing after post is proceeded in 10mL plastic centrifuge tube, after nitrogen dries up, be stored at-20 DEG C for subsequent use.
1.5 field knot hay statistics: based on wild rice stem field growing and harvesting standard, respectively the wild rice stem of 4 treatment combinations is gathered on May 24th, 2013 to June 8.The collecting time first of processing one, processing two, processing three these mulberry processing is May 27, and processing four first collecting time is May 27.While gathering, respectively knot hay number, output, plant height, shell hay weight, hay meat length, hay meat width are measured and added up.
2. interpretation of result
2.1 wild rice stem change of heights
Somatotropin is used rear 4 different times the wild rice stem plant height of 4 treatment combinations is measured and statistical analysis, the results are shown in Table 1.The statistics of table 1 shows: somatotropin treatment combination has very significantly facilitation to wild rice stem plant strain growth, the wild rice stem plant of somatotropin treatment combination is significantly higher than other combinations before pregnant hay, but in the time of picking time and between other treatment combinations, there is no significant difference, the results are shown in Table 5.
Table 1: wild rice stem plant height field statistics
2.2 wild rice stem growth hormone content
With Dot-ELISA to the pregnant hay of wild rice stem before the comparative analysis of basal part of stem IAA content show: the use of somatotropin is little to Various Zizania latifolia IAA in period content influence, the results are shown in Table 2.
Table 2: different disposal combination IAA content before and after processing
2.3 wild rice stem GA content change
With Dot-ELISA to the pregnant hay of wild rice stem before basal part of stem GA
3the comparative analysis of content shows: somatotropin uses Various Zizania latifolia GA in period
3content impact seldom.No significant difference between each combination while using for the first time latter the 7th day, the 14th day, the 21st day; Different disposal combination GA the 28th day time
3content manifests difference, the GA of adjoining tree
3content is significantly higher than the plant of nutrient solution processing.The results are shown in Table 3.
Table 3: different disposal combination GA before and after processing
3content
2.4 wild rice stem ABA contents change
With Dot-ELISA to the pregnant hay of wild rice stem before the comparative analysis of basal part of stem ABA content show: spraying basal part of stem ABA content influence before the pregnant hay of wild rice stem of somatotropin is very remarkable.In most of periods, the ABA content of adjoining tree is significantly higher than other treatment combinations.The results are shown in Table 4.
Table 4: different disposal combination ABA content before and after processing
2.5 field wild rice stem knot hay analyses
The statistic analysis result of different disposal combination wild rice stem knot hay is in table 5, as shown in Table 5: the use of somatotropin can significantly improve the output of wild rice stem.Process one, process two with respect to control group, knot hay number has improved respectively 20.7,10.0 times, and gross yield has improved respectively 25.1,12.6 times.The shell hay representation work of control group combines lower than other, and the hay meat width between different disposal combination does not have notable difference.Hay meat length does not have significant difference between the somatotropin of different formulations and contrast.Compare control group, two formula collecting time evenings of the present invention three days.
Table 5: the pregnant hay situation of wild rice stem after processing
| Process | Process one | Process two | Process three | Process four |
| Collecting time first | May 27 | May 27 | May 27 | May 24 |
| Total number (individual) | 20.67±0.96 b | 10.00±0.13 c | 0.33±0.05 d | 1.00±0.08 d |
| Gross yield (kg) | 2.26±0.09 c | 1.13±0.02 c | 0.04±0.01 c | 0.09±0.01 c |
| Average plant height (cm) | 177.8±3.14 a | 174.8±0.53 a | 165.4±0.33 a | 173.4±0.33 a |
| Shell hay heavy (g) | 53.3±4.92 b | 50.27±5.52 b | 63.20±4.23 ab | 39.74±5.64 c |
| Hay meat length (cm) | 14.50±0.68 ab | 12.80±0.42 b | 16.20±1.01 a | 12.20±0.15 b |
| Hay meat width (cm) | 3.93±0.83 a | 3.16±0.12 a | 3.60±0.12 a | 3.10±0.12 a |
Claims (7)
1. growth hormone wild rice stem progestogenic hay agent, is characterized in that this wild rice stem progestogenic hay agent is composed of the following components: growth hormone, abscisic acid, gibberellin, three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:1-2:1.
2. growth hormone wild rice stem progestogenic hay according to claim 1 agent, is characterized in that this wild rice stem progestogenic hay agent is composed of the following components, growth hormone, abscisic acid, gibberellin, and three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:2:1.
3. growth hormone wild rice stem progestogenic hay according to claim 1 agent, is characterized in that this wild rice stem progestogenic hay agent is composed of the following components, growth hormone, abscisic acid, gibberellin, and three's weight ratio is: growth hormone: abscisic acid: gibberellin=2:1:1.
4. a spraying method for growth hormone wild rice stem progestogenic hay claimed in claim 1 agent, is characterized in that the method is made up of following steps:
1) Jiao Dun selects: select hay Tanaka normally to tie hay and the similar Jiao Dun of proterties in field planting in the last ten-days period in March, conventional field management;
2) preparation of progestogenic hay dilution agent solution: take the somatotropin of formula ratio, dissolve with ethanol, it is 5% that ultra-pure water is settled to mother liquid concentration, when use 1000 times of mother liquor dilute with waters progestogenic hay dilution agent solution;
3) spray: in the 4-5 leaf phase of wild rice stem growth, spray on the blade and leaf sheath of wild rice stem plant with progestogenic hay dilution agent solution, sprayed once every 7 days, spray continuously twice.
5. the spraying method of growth hormone wild rice stem progestogenic hay according to claim 4 agent, is characterized in that step 2) in concentration of alcohol be 95%.
6. the spraying method of growth hormone wild rice stem progestogenic hay according to claim 4 agent, is characterized in that step 2) the w/v g:ml of the agent of progestogenic hay and ethanol is 4-5:2.
7. the spraying method of growth hormone wild rice stem progestogenic hay according to claim 4 agent, is characterized in that in step 3), and the amount of spraying is that every pier wild rice stem sprays 4L progestogenic hay dilution agent solution at every turn.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104585194A (en) * | 2014-12-31 | 2015-05-06 | 安徽农业大学 | Pregnancy-prompting agent for zizania aquatica and method for increasing pregnancy rate and quality of zizania aquatica |
| CN107278570A (en) * | 2017-07-10 | 2017-10-24 | 中国计量大学 | A kind of method for promoting wild rice stem to tie hay in advance and increase production |
| CN107306683A (en) * | 2017-06-28 | 2017-11-03 | 中国计量大学 | A kind of method for postponing wild rice stem knot hay |
| CN107455111A (en) * | 2017-07-10 | 2017-12-12 | 中国计量大学 | A kind of method for promoting wild rice stem to concentrate harvesting |
| CN108887268A (en) * | 2018-06-05 | 2018-11-27 | 中国计量大学 | A kind of growth regulator and its spraying method of the pregnant hay of wild rice stem ahead of time |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585194A (en) * | 2014-12-31 | 2015-05-06 | 安徽农业大学 | Pregnancy-prompting agent for zizania aquatica and method for increasing pregnancy rate and quality of zizania aquatica |
| CN107306683A (en) * | 2017-06-28 | 2017-11-03 | 中国计量大学 | A kind of method for postponing wild rice stem knot hay |
| CN107278570A (en) * | 2017-07-10 | 2017-10-24 | 中国计量大学 | A kind of method for promoting wild rice stem to tie hay in advance and increase production |
| CN107455111A (en) * | 2017-07-10 | 2017-12-12 | 中国计量大学 | A kind of method for promoting wild rice stem to concentrate harvesting |
| CN108887268A (en) * | 2018-06-05 | 2018-11-27 | 中国计量大学 | A kind of growth regulator and its spraying method of the pregnant hay of wild rice stem ahead of time |
| CN108887268B (en) * | 2018-06-05 | 2021-02-09 | 中国计量大学 | Growth regulator for advancing pregnancy of zizania latifolia and spraying method thereof |
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