The cultural method of Ganoderma lucidum mycelium
Technical field
The present invention relates to Ganoderma lucidum mycelium culture technique field, is a kind of technology of cultural method of Ganoderma lucidum mycelium specifically.
Background technology
Glossy ganoderma, the effect of have and have preventing and curing diseases, promoting longevity, wherein GL-B be that glossy ganoderma is strengthened the body resistance to consolidate the constitution, strengthening by means of tonics, the main component of promoting longevity.Glossy ganoderma, can medicine-food two-purpose owing to having no side effect.Nowadays Wild ganoderma resource is also in continuous minimizing.At present, the ganoderma lucidum product on market adopts artificial cultivation mostly, but artificial cultivation is owing to will using agricultural chemicals and chemical method is cultivated bacterial classification, will use agricultural chemicals and control germ insect pest, therefore bring chemical contamination to ganoderma lucidum fruitbody in cultivation management process.And the biological transformation ratio of glossy ganoderma is always all very low, the cycle of Ganoderma Lucidum is long, adds that production technology and management process are complicated and loaded down with trivial detailsly makes production cost cross height to cause benefit low.To this, people have carried out the various culture studies of Ganoderma lucidum mycelium.
Summary of the invention
The object of this invention is to provide a kind of cost low, be of high nutritive value, the cultural method of the harmless Ganoderma lucidum mycelium of environment-protecting asepsis.
Realize below by following technical scheme:
A cultural method for Ganoderma lucidum mycelium, is characterized in that the cultural method of described Ganoderma lucidum mycelium comprises the following steps:
1) configure nutrient solution and carry out sterilizing;
2), to below aforementioned Solution cooling to 30 ℃, then under aseptic condition, inoculate that glossy ganoderma is female to be planted;
3) inoculation being had the female nutrient solution of planting of glossy ganoderma put into design temperature is that the rotating fermentation tank of 25 ℃ to 28 ℃ leaves standstill;
4) fermentation tank rotates fermented and cultured and obtains ripe Ganoderma lucidum mycelium.
Described nutrient solution is natural nutrient fluid, formed by mung bean, red bean, white sesameseed, corn, wheat, millet and rice configuration, then whole materials is broken into 300-500 object powder with cracker, then by the pulvis mixing and water with (50-70): proportional arrangement (30-50) forms.
Time of repose at least 30 hours in described step 3), should make to inoculate the female nutrient solution of planting of glossy ganoderma and contact fully with air in step 4).
Described mung bean, red bean, white sesameseed, corn, wheat, millet and rice are pressed the configuration of row parts by weight: mung bean 8-12, red bean 5-7, white sesameseed 3-4, corn 23-27, wheat 23-27, millet 13-16 and rice 13-16.
Concrete proportioning has following scheme:
13 parts, 8 parts, described mung bean, 5 parts of red beans, 3 parts of white sesameseeds, 23 parts of corns, 23 parts of wheats, 13 parts of millets and rice.
16 parts, 12 parts, described mung bean, 7 parts of red beans, 4 parts of white sesameseeds, 27 parts of corns, 27 parts of wheats, 16 parts of millets and rice.
15 parts, 8 parts, described mung bean, 5 parts of red beans, 3 parts of white sesameseeds, 26 parts of corns, 26 parts of wheats, 15 parts of millets and rice.
13 parts, 9 parts, described mung bean, 6 parts of red beans, 3 parts of white sesameseeds, 26 parts of corns, 26 parts of wheats, 16 parts of millets and rice.
The present invention compared with prior art has the following advantages.
The present invention is owing to adopting mung bean, red bean, white sesameseed, corn, wheat, millet and rice to configure the natural nutrient fluid forming as medium, make the nutrient source of the Ganoderma lucidum mycelium of cultivating more abundant and comprehensive, be conducive to mycelia and absorb and breed, avoided cultivating brought defect with single corn.The present invention does not have source of heavy metal pollution, and cost is low, is of high nutritive value, and environment-protecting asepsis is harmless.Particularly adopt fermentation tank to rotate fermented and cultured, aseptic oxygen is fully utilized, and is connected to the female nutrient solution of planting of glossy ganoderma and contacts more fully with air, has greatly improved the biological transformation ratio of glossy ganoderma, has shortened cultivation cycle, makes production technology and management process become simple.The configuration raw material of nutrient solution of the present invention is extensive, and cost is low, is conducive to the popularization of technology, and the glossy ganoderma natural health of cultivating with this natural grain.
Embodiment
Below the present invention is described in further detail.
The cultural method of Ganoderma lucidum mycelium of the present invention, comprises the following steps:
1) configure nutrient solution and carry out sterilizing;
2), to below aforementioned Solution cooling to 30 ℃, then under aseptic condition, inoculate that glossy ganoderma is female to be planted;
3) inoculation being had the female nutrient solution of planting of glossy ganoderma put into design temperature is that the rotating fermentation tank of 25 ℃ to 28 ℃ leaves standstill;
4) fermentation tank rotates fermented and cultured and obtains ripe Ganoderma lucidum mycelium.
Abovementioned steps 3) in time of repose at least 30 hours, in step 4), should make to inoculate the female nutrient solution of planting of glossy ganoderma and contact fully with air.Wherein nutrient solution is natural nutrient fluid, formed by mung bean, red bean, white sesameseed, corn, wheat, millet and rice configuration, then whole materials is broken into 300-500 object powder with cracker, then by the pulvis mixing and water with (50-70): proportional arrangement (30-50) forms.Wherein rice can be thick rice.Millet in the present invention, its formal name used at school: Setaria italica, Ancient Chinese claims millet or grain.The grain that shelling is made, because it is little, diameter 2 millimeter, therefore named.Originate in the north of China Huanghe valley, the staple food crop of ancient Chinese, so Xia dynasty and Shang dynasty belong to " grain culture ".Grain growth is drought-enduring, various in style, is commonly called as " grain has the five colours ", has white, red, yellow, black, orange, purple versicolor millet, also toughness millet.China's wine the earliest is also brewageed with millet.Grain is adapted at arid and lacks the area growth of irrigation.Its stem, leaf are harder, can make feed, generally only have ox to digest.Grain is commonly called as millet at north of China.Western language generally has general designation to grain, broomcorn millet, cattailmillet and the little coarse cereals of some other grain, non-agricultural expert generally regardless of, as English all claims " millet ".
The present invention's more detailed description is: first preparing natural nutrient solution, and then under 1.5 kilograms of pressure, sterilizing more than 2 hours, is cooled to while reaching aseptic requirement below 30 degree, and then under aseptic condition, conventional inoculation method access glossy ganoderma mother plants.Inoculation is had to the rotatable fermentation tank that design temperature is 25 to 28 degree of putting into of female nutrient solution of planting, leaving standstill 30 hours after fermentation tanks starts slowly to rotate and is beneficial in tank object and exchanges up and down and increased oxygen, when rotation, aseptic oxygen is fully utilized, the nutrient solution that is connected to female bacterium contacts more abundant with air, so fermentation 7 to ten days, has a large amount of ripe Ganoderma lucidum myceliums in fermentation tank.The new fresh thalli of the overanxious rear results of centrifuge can be used for producing.
In the present invention, mung bean, red bean, white sesameseed, corn, wheat, millet and rice are pressed the configuration of row parts by weight: mung bean 8-12, red bean 5-7, white sesameseed 3-4, corn 23-27, wheat 23-27, millet 13-16 and rice 13-16.
Concrete proportioning has following scheme: can be kilogram to be configured as unit.Specifically how much be configured according to aequum.
13 parts, 8 parts, mung bean, 5 parts of red beans, 3 parts of white sesameseeds, 23 parts of corns, 23 parts of wheats, 13 parts of millets and rice;
16 parts, 12 parts, mung bean, 7 parts of red beans, 4 parts of white sesameseeds, 27 parts of corns, 27 parts of wheats, 16 parts of millets and rice;
15 parts, 8 parts, mung bean, 5 parts of red beans, 3 parts of white sesameseeds, 26 parts of corns, 26 parts of wheats, 15 parts of millets and rice;
13 parts, 9 parts, mung bean, 6 parts of red beans, 3 parts of white sesameseeds, 26 parts of corns, 26 parts of wheats, 16 parts of millets and rice.