CN103875448A - Culturing method for ganoderma lucidum mycelia - Google Patents
Culturing method for ganoderma lucidum mycelia Download PDFInfo
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- CN103875448A CN103875448A CN201410095636.9A CN201410095636A CN103875448A CN 103875448 A CN103875448 A CN 103875448A CN 201410095636 A CN201410095636 A CN 201410095636A CN 103875448 A CN103875448 A CN 103875448A
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- parts
- ganoderma lucidum
- nutrient solution
- rice
- lucidum mycelium
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- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 29
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000012258 culturing Methods 0.000 title abstract 4
- 244000062793 Sorghum vulgare Species 0.000 claims abstract description 30
- 235000019713 millet Nutrition 0.000 claims abstract description 30
- 235000015097 nutrients Nutrition 0.000 claims abstract description 29
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 25
- 235000009566 rice Nutrition 0.000 claims abstract description 25
- 240000008042 Zea mays Species 0.000 claims abstract description 24
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 24
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 24
- 235000005822 corn Nutrition 0.000 claims abstract description 24
- 235000021307 Triticum Nutrition 0.000 claims abstract description 23
- 240000001417 Vigna umbellata Species 0.000 claims abstract description 23
- 235000011453 Vigna umbellata Nutrition 0.000 claims abstract description 23
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 239000000463 material Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 241000209094 Oryza Species 0.000 claims description 24
- 241000209140 Triticum Species 0.000 claims description 22
- 240000004922 Vigna radiata Species 0.000 claims description 22
- 235000010721 Vigna radiata var radiata Nutrition 0.000 claims description 22
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 claims description 22
- 241000222336 Ganoderma Species 0.000 claims description 18
- 208000003643 Callosities Diseases 0.000 claims description 12
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 239000002245 particle Substances 0.000 abstract 2
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 244000000231 Sesamum indicum Species 0.000 abstract 1
- 235000003434 Sesamum indicum Nutrition 0.000 abstract 1
- 244000098338 Triticum aestivum Species 0.000 abstract 1
- 244000013123 dwarf bean Species 0.000 abstract 1
- 235000021331 green beans Nutrition 0.000 abstract 1
- 239000002574 poison Substances 0.000 abstract 1
- 231100000614 poison Toxicity 0.000 abstract 1
- 235000013339 cereals Nutrition 0.000 description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 239000003905 agrochemical Substances 0.000 description 2
- 238000004500 asepsis Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 235000007199 Panicum miliaceum Nutrition 0.000 description 1
- 244000115721 Pennisetum typhoides Species 0.000 description 1
- 235000007195 Pennisetum typhoides Nutrition 0.000 description 1
- 235000008515 Setaria glauca Nutrition 0.000 description 1
- 240000005498 Setaria italica Species 0.000 description 1
- 235000007226 Setaria italica Nutrition 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000022185 broomcorn panic Species 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 235000015961 tonic Nutrition 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a culturing method for ganoderma lucidum mycelia. The culturing method includes the following steps that (1), a nutrient solution is prepared and sterilized is performed on the nutrient solution; (2), the nutrient solution is cooled below 30 DEG C, and then ganoderma lucidum mother plants are inoculated under the sterile condition; (3), the nutrient solution where the ganoderma lucidum mother plants are inoculated is placed in a rotatable fermentation tank to stand, where the fermentation tank is set to be at the temperature of 25 DEG C-28 DEG C; (4), the fermentation tank rotates to perform fermentation cultivation, so that mature ganoderma lucidum mycelia are obtained, the nutrient solution is natural nutrient solution and formed by preparing green beans, red beans, white sesame seeds, corn, wheat, millet and rice, then all the materials are pulverized to particles in 300-500 meshes through a pulverizer, and then the particles and water are mixed according to the proportion of (50-70): (30-50). The culturing method is low in cost, high in nutrient value, environmentally friendly and free of poison and harm.
Description
Technical field
The present invention relates to Ganoderma lucidum mycelium culture technique field, is a kind of technology of cultural method of Ganoderma lucidum mycelium specifically.
Background technology
Glossy ganoderma, the effect of have and have preventing and curing diseases, promoting longevity, wherein GL-B be that glossy ganoderma is strengthened the body resistance to consolidate the constitution, strengthening by means of tonics, the main component of promoting longevity.Glossy ganoderma, can medicine-food two-purpose owing to having no side effect.Nowadays Wild ganoderma resource is also in continuous minimizing.At present, the ganoderma lucidum product on market adopts artificial cultivation mostly, but artificial cultivation is owing to will using agricultural chemicals and chemical method is cultivated bacterial classification, will use agricultural chemicals and control germ insect pest, therefore bring chemical contamination to ganoderma lucidum fruitbody in cultivation management process.And the biological transformation ratio of glossy ganoderma is always all very low, the cycle of Ganoderma Lucidum is long, adds that production technology and management process are complicated and loaded down with trivial detailsly makes production cost cross height to cause benefit low.To this, people have carried out the various culture studies of Ganoderma lucidum mycelium.
Summary of the invention
The object of this invention is to provide a kind of cost low, be of high nutritive value, the cultural method of the harmless Ganoderma lucidum mycelium of environment-protecting asepsis.
Realize below by following technical scheme:
A cultural method for Ganoderma lucidum mycelium, is characterized in that the cultural method of described Ganoderma lucidum mycelium comprises the following steps:
1) configure nutrient solution and carry out sterilizing;
2), to below aforementioned Solution cooling to 30 ℃, then under aseptic condition, inoculate that glossy ganoderma is female to be planted;
3) inoculation being had the female nutrient solution of planting of glossy ganoderma put into design temperature is that the rotating fermentation tank of 25 ℃ to 28 ℃ leaves standstill;
4) fermentation tank rotates fermented and cultured and obtains ripe Ganoderma lucidum mycelium.
Described nutrient solution is natural nutrient fluid, formed by mung bean, red bean, white sesameseed, corn, wheat, millet and rice configuration, then whole materials is broken into 300-500 object powder with cracker, then by the pulvis mixing and water with (50-70): proportional arrangement (30-50) forms.
Time of repose at least 30 hours in described step 3), should make to inoculate the female nutrient solution of planting of glossy ganoderma and contact fully with air in step 4).
Described mung bean, red bean, white sesameseed, corn, wheat, millet and rice are pressed the configuration of row parts by weight: mung bean 8-12, red bean 5-7, white sesameseed 3-4, corn 23-27, wheat 23-27, millet 13-16 and rice 13-16.
Concrete proportioning has following scheme:
13 parts, 8 parts, described mung bean, 5 parts of red beans, 3 parts of white sesameseeds, 23 parts of corns, 23 parts of wheats, 13 parts of millets and rice.
16 parts, 12 parts, described mung bean, 7 parts of red beans, 4 parts of white sesameseeds, 27 parts of corns, 27 parts of wheats, 16 parts of millets and rice.
15 parts, 8 parts, described mung bean, 5 parts of red beans, 3 parts of white sesameseeds, 26 parts of corns, 26 parts of wheats, 15 parts of millets and rice.
13 parts, 9 parts, described mung bean, 6 parts of red beans, 3 parts of white sesameseeds, 26 parts of corns, 26 parts of wheats, 16 parts of millets and rice.
The present invention compared with prior art has the following advantages.
The present invention is owing to adopting mung bean, red bean, white sesameseed, corn, wheat, millet and rice to configure the natural nutrient fluid forming as medium, make the nutrient source of the Ganoderma lucidum mycelium of cultivating more abundant and comprehensive, be conducive to mycelia and absorb and breed, avoided cultivating brought defect with single corn.The present invention does not have source of heavy metal pollution, and cost is low, is of high nutritive value, and environment-protecting asepsis is harmless.Particularly adopt fermentation tank to rotate fermented and cultured, aseptic oxygen is fully utilized, and is connected to the female nutrient solution of planting of glossy ganoderma and contacts more fully with air, has greatly improved the biological transformation ratio of glossy ganoderma, has shortened cultivation cycle, makes production technology and management process become simple.The configuration raw material of nutrient solution of the present invention is extensive, and cost is low, is conducive to the popularization of technology, and the glossy ganoderma natural health of cultivating with this natural grain.
Embodiment
Below the present invention is described in further detail.
The cultural method of Ganoderma lucidum mycelium of the present invention, comprises the following steps:
1) configure nutrient solution and carry out sterilizing;
2), to below aforementioned Solution cooling to 30 ℃, then under aseptic condition, inoculate that glossy ganoderma is female to be planted;
3) inoculation being had the female nutrient solution of planting of glossy ganoderma put into design temperature is that the rotating fermentation tank of 25 ℃ to 28 ℃ leaves standstill;
4) fermentation tank rotates fermented and cultured and obtains ripe Ganoderma lucidum mycelium.
Abovementioned steps 3) in time of repose at least 30 hours, in step 4), should make to inoculate the female nutrient solution of planting of glossy ganoderma and contact fully with air.Wherein nutrient solution is natural nutrient fluid, formed by mung bean, red bean, white sesameseed, corn, wheat, millet and rice configuration, then whole materials is broken into 300-500 object powder with cracker, then by the pulvis mixing and water with (50-70): proportional arrangement (30-50) forms.Wherein rice can be thick rice.Millet in the present invention, its formal name used at school: Setaria italica, Ancient Chinese claims millet or grain.The grain that shelling is made, because it is little, diameter 2 millimeter, therefore named.Originate in the north of China Huanghe valley, the staple food crop of ancient Chinese, so Xia dynasty and Shang dynasty belong to " grain culture ".Grain growth is drought-enduring, various in style, is commonly called as " grain has the five colours ", has white, red, yellow, black, orange, purple versicolor millet, also toughness millet.China's wine the earliest is also brewageed with millet.Grain is adapted at arid and lacks the area growth of irrigation.Its stem, leaf are harder, can make feed, generally only have ox to digest.Grain is commonly called as millet at north of China.Western language generally has general designation to grain, broomcorn millet, cattailmillet and the little coarse cereals of some other grain, non-agricultural expert generally regardless of, as English all claims " millet ".
The present invention's more detailed description is: first preparing natural nutrient solution, and then under 1.5 kilograms of pressure, sterilizing more than 2 hours, is cooled to while reaching aseptic requirement below 30 degree, and then under aseptic condition, conventional inoculation method access glossy ganoderma mother plants.Inoculation is had to the rotatable fermentation tank that design temperature is 25 to 28 degree of putting into of female nutrient solution of planting, leaving standstill 30 hours after fermentation tanks starts slowly to rotate and is beneficial in tank object and exchanges up and down and increased oxygen, when rotation, aseptic oxygen is fully utilized, the nutrient solution that is connected to female bacterium contacts more abundant with air, so fermentation 7 to ten days, has a large amount of ripe Ganoderma lucidum myceliums in fermentation tank.The new fresh thalli of the overanxious rear results of centrifuge can be used for producing.
In the present invention, mung bean, red bean, white sesameseed, corn, wheat, millet and rice are pressed the configuration of row parts by weight: mung bean 8-12, red bean 5-7, white sesameseed 3-4, corn 23-27, wheat 23-27, millet 13-16 and rice 13-16.
Concrete proportioning has following scheme: can be kilogram to be configured as unit.Specifically how much be configured according to aequum.
13 parts, 8 parts, mung bean, 5 parts of red beans, 3 parts of white sesameseeds, 23 parts of corns, 23 parts of wheats, 13 parts of millets and rice;
16 parts, 12 parts, mung bean, 7 parts of red beans, 4 parts of white sesameseeds, 27 parts of corns, 27 parts of wheats, 16 parts of millets and rice;
15 parts, 8 parts, mung bean, 5 parts of red beans, 3 parts of white sesameseeds, 26 parts of corns, 26 parts of wheats, 15 parts of millets and rice;
13 parts, 9 parts, mung bean, 6 parts of red beans, 3 parts of white sesameseeds, 26 parts of corns, 26 parts of wheats, 16 parts of millets and rice.
Claims (8)
1. a cultural method for Ganoderma lucidum mycelium, is characterized in that the cultural method of described Ganoderma lucidum mycelium comprises the following steps:
1) configure nutrient solution and carry out sterilizing;
2), to below aforementioned Solution cooling to 30 ℃, then under aseptic condition, inoculate that glossy ganoderma is female to be planted;
3) inoculation being had the female nutrient solution of planting of glossy ganoderma put into design temperature is that the rotating fermentation tank of 25 ℃ to 28 ℃ leaves standstill;
4) fermentation tank rotates fermented and cultured and obtains ripe Ganoderma lucidum mycelium.
2. described in, nutrient solution is natural nutrient fluid, formed by mung bean, red bean, white sesameseed, corn, wheat, millet and rice configuration, then whole materials is broken into 300-500 object powder with cracker, then by the pulvis mixing and water with (50-70): proportional arrangement (30-50) forms.
3. the cultural method of Ganoderma lucidum mycelium according to claim 1, is characterized in that: time of repose at least 30 hours in described step 3), should make to inoculate the female nutrient solution of planting of glossy ganoderma and contact fully with air in step 4).
4. the cultural method of Ganoderma lucidum mycelium according to claim 1, is characterized in that: described mung bean, red bean, white sesameseed, corn, wheat, millet and rice are pressed the configuration of row parts by weight: mung bean 8-12, red bean 5-7, white sesameseed 3-4, corn 23-27, wheat 23-27, millet 13-16 and rice 13-16.
5. the cultural method of Ganoderma lucidum mycelium according to claim 1, is characterized in that: 13 parts, 8 parts, described mung bean, 5 parts of red beans, 3 parts of white sesameseeds, 23 parts of corns, 23 parts of wheats, 13 parts of millets and rice.
6. the cultural method of Ganoderma lucidum mycelium according to claim 1, is characterized in that: 16 parts, 12 parts, described mung bean, 7 parts of red beans, 4 parts of white sesameseeds, 27 parts of corns, 27 parts of wheats, 16 parts of millets and rice.
7. the cultural method of Ganoderma lucidum mycelium according to claim 1, is characterized in that: 15 parts, 8 parts, described mung bean, 5 parts of red beans, 3 parts of white sesameseeds, 26 parts of corns, 26 parts of wheats, 15 parts of millets and rice.
8. the cultural method of Ganoderma lucidum mycelium according to claim 1, is characterized in that: 13 parts, 9 parts, described mung bean, 6 parts of red beans, 3 parts of white sesameseeds, 26 parts of corns, 26 parts of wheats, 16 parts of millets and rice.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410095636.9A CN103875448B (en) | 2014-03-17 | 2014-03-17 | The cultural method of Ganoderma lucidum mycelium |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410095636.9A CN103875448B (en) | 2014-03-17 | 2014-03-17 | The cultural method of Ganoderma lucidum mycelium |
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| CN103875448A true CN103875448A (en) | 2014-06-25 |
| CN103875448B CN103875448B (en) | 2017-12-01 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957514A (en) * | 2015-07-09 | 2015-10-07 | 淮阴师范学院 | Method for preparing lucid ganoderma rice meal by utilizing ganoderma lucidum mycelia to ferment rice |
| CN105309197A (en) * | 2014-07-26 | 2016-02-10 | 雷色香 | Preparation method of lucid ganoderma rice |
| CN107047061A (en) * | 2017-04-21 | 2017-08-18 | 广西壮族自治区农业科学院微生物研究所 | The high efficiency method and its culture medium of cultivation of glossy ganoderma |
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2014
- 2014-03-17 CN CN201410095636.9A patent/CN103875448B/en not_active Expired - Fee Related
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105309197A (en) * | 2014-07-26 | 2016-02-10 | 雷色香 | Preparation method of lucid ganoderma rice |
| CN104957514A (en) * | 2015-07-09 | 2015-10-07 | 淮阴师范学院 | Method for preparing lucid ganoderma rice meal by utilizing ganoderma lucidum mycelia to ferment rice |
| CN107047061A (en) * | 2017-04-21 | 2017-08-18 | 广西壮族自治区农业科学院微生物研究所 | The high efficiency method and its culture medium of cultivation of glossy ganoderma |
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