CN103766650A - Laying hen feed additive and preparation thereof - Google Patents
Laying hen feed additive and preparation thereof Download PDFInfo
- Publication number
- CN103766650A CN103766650A CN201310736910.1A CN201310736910A CN103766650A CN 103766650 A CN103766650 A CN 103766650A CN 201310736910 A CN201310736910 A CN 201310736910A CN 103766650 A CN103766650 A CN 103766650A
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- feed addictive
- layer chicken
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- bacillus subtilis
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Abstract
The invention discloses a laying hen feed additive and belongs to the technical field of feed additives. The laying hen feed additive is composed of following components in parts by weight: 35-65 parts of corns, 5-10 parts of rice bran, 4-9 parts of wheat bran, 5-9 parts of peanut meal, 0.5-3 parts of plant oil, 0.2-0.4 part of table salt, 0.1-0.2 part of a calcium element additive, 0.4-2.6 parts of amino acid, 0.05 part of a complex enzyme preparation, 0.012 part of phytase, 0.05-0.12 part of choline chloride, 50-150 parts of vitamin E, 1-5 parts of cordyceps taishanensis powder, 2-6 parts of asparagus cochinchinensis powder, 0.5-1 part of glycyrrhiza uralensis powder, 5-8 parts of a bacillus subtilis CGMCC7926 (China General Microbiological Culture Collection Center 7926) bacterium agent culture, 3-6 parts of a lactobacillus plantarum CGMCC7928 bacterium agent and 1-2 parts of a saccharomyces cerevisiae bacterium agent. The laying hen feed additive sufficiently guarantees that propagative organs of laying hens are completely and rapidly developed; eggs laid on an egg laying initial phase are heavy and large and the peak is high and the egg laying peak is maintained for long time.
Description
Technical field
The present invention relates to a kind of feed addictive, belong to feed additive field, particularly a kind of feed addictive of layer chicken and preparation thereof.
Background technology
Feed is the prime cost of animal-breeding, and animal-breeding cost has 60%-70% to come from feed cost use according to statistics, therefore reduces feed waste, improves efficiency of feed utilization, is the key point of carrying out aquaculture cost control and improving culture benefit.At present the common efficiency of feed utilization that exists of aquaculture is not high, and not exclusively, keeping is not good at causing feed moisture absorption to go mouldy or the problem such as infested in nutritional labeling digestion, and the existence of these problems has not only increased feeding cost greatly, and has caused the wasting of resources.How to address these problems, control aquaculture cost, reducing cultivation risk is the large bottleneck of one in aquaculture fast development.There is in recent years the much research about improving efficiency of feed utilization, use chemical synthesis additive agent field but mainly concentrate on, chemical synthesis additive can cause animal drug resistance, the problem such as residual, harm humans health, also has a large amount of excretas simultaneously, as the pernicious gas such as ammonia, hydrogen sulfide, contaminated environment.Chinese herbal feed additive is as the focus of emerging research, has the advantages such as natural, nutrition, side effect is little.Devoting Major Efforts To Developing Chinese herbal feed additive is to solving antibiotic residue problem, boost productivity, development stockbreeding industry, meet people's food security demand, dwindle China's animal husbandry and developed country's gap, strengthen the competitiveness of China's livestock products in international market, there is important economic implications and social benefit.Research shows, many Chinese herbal medicines itself just have good In Vitro Bacteriostasis ability, different from Western medicine is, Chinese herbal feed antibiotic health care agent noresidue, have no drug resistance, its mechanism of action is not only the inhibitory or killing effect to pathogenic microorganism, the more important thing is the adjustment to body disease-resistant repair ability, improve anti-stress ability, raising body's immunity and the Defense response function of body, thereby reach the object that improves breeding performonce fo animals and food utilization efficiency.
Chinese herbal medicine is some plants with given efficacy, these plants are each powerful, the effectiveness of for example Activities of Some Plants is as follows: lucid asparagus: its medicinal part is mainly piece root, there is replenishing the vital essence and removing heat, moisturize the effect of promoting the production of body fluid, modern pharmacology research shows that it has good curative effect to symptoms such as pulmonary tuberculosis, bronchitis, diphtheria, pertussis, dry mouth and throats, also has antibacterial immunity and anti-oxidation function simultaneously.Fructus Corni: its taste acid, puckery, tepor, returns liver kidney channel.Contain various active composition, comprise volatile ingredient, glucosides class and aglycon, tannin and organic acid etc.Fructus Corni water decoction can significantly raise mice serum hemolytic antibody and serum antibody IgG content.Carbohydrate in Fructus Corni has the effect of obvious Promote immunity reaction.Cattail pollen: be the dry pollen of Typhaceae plant raupo cattail, typha orientalis, theory of traditional Chinese medical science thinks that this property of medicine taste is sweet flat, returns liver, the heart, the spleen channel, effect of tool analgesia stagnation resolvation.Echinacea: originate in a class Echinacea plant in North America and Canada south, its main active is polysaccharide, alkylamide compound and Caffeic acids derivative.Research shows that different xylan and the arabinose in Echinacea can pass through to stimulate the activity of the lymphocytic propagation of monokaryon and macrophage, thereby has significant immunostimulation.Echinacea also has antibacterial and anti-inflammation functions, and its active component Cichoric acid has the immune anti-inflammatory properties of enhancing, can suppress hyaluronic acid, and protection collagen III is avoided degraded, and Escherichia coli are had to obvious inhibitory action.
How effectively to utilize existing herbal raw material preparation can effectively improve the feed addictive of animal immunizing power and the compound utilization ratio of raising feed, the high value utilization that realizes feed is one and is conducive to the project that feed industry develops in a healthy way.
Summary of the invention:
The object of the invention is to develop a kind of feed addictive of layer chicken and preparation method thereof.
Technical scheme of the present invention is as follows:
A kind of feed addictive of layer chicken, parts by weight consist of: corn 35-65 part, rice bran 5-10 part, wheat bran 4-9 part, peanut meal 5-9 part, vegetable oil 0.5-3 part, salt 0.2-0.4 part, calcium constituent additive 0.1-0.2 part, amino acid 0.4-2.6 part, 0.05 part of complex enzyme formulation, 0.012 part of phytase, Choline Chloride 0.05-0.12 part, vitamin E 50-150 part, cordyceps 1-5 part, Tianmen Green bean noodle 2-6 part, licorice powder 0.5-1 part, bacillus subtilis CGMCC7926 microbial inoculum culture 5-8 part, Lactobacillus plantarum CGMCC7928 microbial inoculum 3-6 part, saccharomyces cerevisiae microbial inoculum 1-2 part.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer.
Described licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer.
Described cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step and obtains zymotic fluid, and zymotic fluid centrifugation obtains mycelia, and mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 08%, Fructus Corni 0.7%, peptone 0.4%, soyabean protein powder 0.6%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0.
Described calcium constituent additive is a kind of in calcium carbonate, calcium monohydrogen phosphate or calcium dihydrogen phosphate or two kinds.
Described amino acid is two or more the mixing in lysine, methionine, threonine or tryptophan.
In described complex enzyme formulation, the parts by weight content of each component is as follows: zytase 3-6 part, mannase 1-2 part, protease 1-3 part;
The unit of above-mentioned enzyme is as follows: zytase 20000-30000U/g, mannase 1000-40000U/g, protease 3 000-5000U/g.
Bacillus subtilis culture preparation: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation is step by step transferred in fermentation tank, and control temperature is 28-30 ℃, aerlbic culture 19-24 hour, throughput is 2.0m
3/ minute; Zymotic fluid plate-frame filtering, the culture that dry acquisition contains bacillus subtilis.
Saccharomyces cerevisiae microbial inoculum preparation: slant strains obtains yeast starter liquid through the multistage technique that spreads cultivation of routine, transfers in fermentation tank, and controlling temperature is 28 ℃, aerlbic culture 14 hours in earlier stage, throughput control is 2.0m
3/ minute, later stage anaerobism is cultivated 15 hours; Zymotic fluid low temperature is concentrated, and after mixing with carrier, through fluidized bed drying preparation, vehicle group becomes CaCO
340 parts, 20 parts, dextrin, 20 parts of corn protein powders.
Lactobacillus plantarum preparation: inclined-plane is cultivated Lactobacillus plantarum seed liquor and transferred in fermentation tank, and control temperature is 28-32 ℃, anaerobism is cultivated 22 hours, and throughput is 2.0m
3/ minute; The complete centrifugation of fermenting obtains wet thallus, adds the protective agent that consists of 10% defatted milk, 5% trehalose, 5% glycerine, obtains microbial inoculum by freeze drying, and moisture is lower than 10%.
The preparation method of product of the present invention is as follows: Tianmen Green bean noodle and licorice powder after pulverizing are mixed with other raw materials according to formula rate.
The invention has the beneficial effects as follows:
In feed, add bacillus subtilis culture, the composite bacillus subtilis microbial agent of science of the present invention, Lactobacillus plantarum microbial inoculum and saccharomyces cerevisiae microbial inoculum, bacillus subtilis, saccharomyces cerevisiae microbial inoculum and Lactobacillus plantarum microbial inoculum have been realized to organic assembling, in chicken stomach, under effect due to body temperature factor, enzyme preparation starts to have an effect, and decomposes wherein nutriment; Lactobacillus plantarum body fluid and around after digestion trophic factors start growth and breeding and produce lactic acid, the distinctive strong galactopoiesis acidity of Lactobacillus plantarum is brought into play, the generation of lactic acid has promoted and has formed the digestive environments of stomach effectively, promotes digesting and assimilating of nutriment; The factors such as product bacillus subtilis of the present invention enter enteron aisle and can effectively reduce the usage quantity of antibiotics in letting animals feed, improve the immunity of letting animals feed, improve the security of animal meat product, improve the food utilization efficiency of animal, the use of antibiotic and medicine in the conventional raising of minimizing, strengthen the resistivity of animal, improve raise benefit.
Hen secondary sex characters cockscomb is grown and red is looked soon fast, and wattle is also red looks fast soon, and whole chicken group cockscomb reddens rapidly greatly, has soon and well medium above correlation with organ of multiplication growth.One of target that the present invention reaches is fully to guarantee the growth completely rapidly of laying hen organ of multiplication, and body weight gain is fast, guarantees that physique is strong, for stable high yield is taken a firm foundation; Be embodied in the maximum body weight gains of laying hen, maximum organ of multiplication is grown, and guarantees physical efficiency deposit (energy and calcium phosphorus), is getting rid of hat phase lay heavily!
The present invention adopts science compound feed, coordinates the organic assembling of accurate scale of feeding, fully guarantees the growth completely rapidly of laying hen organ of multiplication, body weight gain is fast, guarantee that physique is strong, body maturation is synchronizeed and is grown with sexal maturity, thereby reaching the initial stage of laying eggs lays eggs great, fast on peak, on height, and it is lasting to maintain the peak of laying eggs, and reaches 7 months and lay eggs above peak, lay eggs 72 week age and reach 311 pieces of eggs, egg production is more than 19.6 kilograms.28 weekend 61.3 grams of average egg weights, improve 3.03% than average level; Laying rate reaches more than 95%, improves 1-3.6% than average level; Egg production, from 48.2 grams/only/day, is brought up to 50.1 grams/only/day; Within 16 weeks, an age-28 week age grade section death rate is reduced to 0.75%, 3 pieces, every chicken fecund egg.
The specific embodiment
Below by specific embodiment narration the present invention.Unless stated otherwise, in the present invention, technological means used is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, do not deviating under the prerequisite of essence of the present invention and scope various changes that the material component in these embodiments and consumption are carried out or change and also belong to protection scope of the present invention.
Saccharomyces cerevisiae saccharomyces cerevisiae CICC31481 provided by the present invention is purchased from Chinese industrial microorganism fungus kind preservation administrative center.
Bacillus subtilis (Bacillus subtilis) Li-2013-02, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 15th, 2013 and (is called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute), preserving number is CGMCC No.7926.
Described strain characteristic is that the enzyme activity of the high temperature resistant AMS of product is high, heat-resisting, acid resistance is strong.
High temperature resistant AMS enzyme activity prepared by described bacterial strain is 30000-35000u/ml; Applicable temperature scope is 105-115 ℃, and 110 ℃ of optimal reactive temperatures, at 110 ℃ of enzymes complete stability alive; Being suitable for pH value in reaction scope is 3.0-7.0, is 3.0 o'clock enzyme complete stabilities alive in pH value, and optimal reaction pH value is 4.2.
Described bacterial strain feature is as follows:
Described bacterial strain colony colour on solid plate is milky, and dry tack free is opaque, and neat in edge, for having the aerobic bacteria of motility.Microscopy is elongated rod shape, and Gram's staining is positive.This bacterium can utilize citrate, and nitrate reductase, V-P test into positive.
Described bacillus subtilis (Bacillus subtilis) Li-2013-02 is produced high temperature resistant AMS bacillus subtilis Li-2013 by a strain obtains through UV-LiCl-dithyl sulfate Mutation screening, specifically screens step as follows:
(1) preparation of bacteria suspension
By in mono-the Li-2013 growing after plate streaking separates bacterium colony access seed culture medium, 100r/min, cultivates after 12h for 40 ℃, uses physiological saline washed twice after getting 1mL medium centrifugal, and in resuspended and 9mL physiological saline.
(2) UV-LiCl-dithyl sulfate complex mutation
Bacteria suspension is placed in to aseptic flat board, is 30cm in distance, stirs and irradiate 100s under the uviol lamp of power 15w.To after gradient dilution, coat lithium chloride flat board through the bacterium liquid irradiating, and contrast with the bacterium liquid dilution painting flat board without ultraviolet irradiation.Above-mentioned coating is dull and stereotyped uniformly, wrap with cloth or the newspaper of black, put 40 ℃ and cultivate 48h, on the flat board that grows bacterium colony, filtering out hydrolysis circle chooses to inclined-plane and preserves with colony diameter ratio the maximum, after purifying, be mixed with bacteria suspension, after gradient dilution, fully mix with dithyl sulfate stoste, and process 40min in 40 ℃ of concussions, the bacterium liquid of processing is coated to lithium chloride flat board after gradient dilution.
(3) primary dcreening operation of high-yield strains
Above-mentioned coating is dull and stereotyped uniformly, put 40 ℃ and cultivate 48h, on the flat board that grows bacterium colony, primary dcreening operation goes out hydrolysis circle and chooses to inclined-plane and preserve with colony diameter ratio the greater, obtains three strain bacterium Li-2013-01, Li-2013-02, Li-2013-03 after purifying
(4) shake flask fermentation sieves again
By the three strain bacterium Li-2013-01 that obtain, Li-2013-02, Li-2013-03 carries out shake flask fermentation in the 250mL shaking flask that contains 30mL fermentation medium, seed inoculum concentration 10% (V/V), 40 ℃, 100r/min are cultivated 72h, and centrifuging and taking fermented supernatant fluid makes crude enzyme liquid.
(5) enzyme activity determination
The definition of Mei Huo unit: 1mL crude enzyme liquid, under 105 ℃, pH4.2 condition, 1min liquefaction 1mg soluble starch,
Be 1 enzyme activity unit, represent with U/mL.
After measured, bacterial strain Li-2013-02, be stable superior strain, and enzyme work reaches 30000U/mL.
Described lithium chloride flat board: starch 1%, peptone 1%, (NH)
2sO
40.4%, K
2hPO
40.8%, CaCl
20.2%, lithium chloride 0.9%, agar 2%.
Described seed culture medium: dusty yeast 0.5%, peptone 1%, soluble starch 1%, NaCl1%.
Described fermentation medium: corn flour 5%-15%, beancake powder 4%-10%, (NH)
2sO
40.4%, K
2hPO
40.8%, CaCl
20.2%.
Described shaking flask condition of culture: this bacterium in the 250mL shaking flask that contains 30mL fermentation medium, inoculum concentration 10% (V/V), 100r/min, 40 ℃ of fermented and cultured 72h.
Described high-temperature resistant alpha-amylase, its zymologic property is as follows:
(1) this enzyme temperature accommodation is wider, and optimum temperature is between 100-110 ℃, and 110 ℃ of following preserve, temperature stability is better, and it is poor more than 110 ℃ to preserve long-time temperature stability.
(2) this enzyme optimal reaction pH value is 4.2.Between pH value 3.0-7.0, all having high enzyme vigor, is 3.0 o'clock enzyme complete stabilities alive in pH value.
(3) enzymatic activity: by mutant strain Li-2013-02 provided by the present invention, the high temperature resistant AMS enzyme activity of preparation is 30000-35000U/ml.
Lactobacillus provided by the present invention is Lactobacillus plantarum (Lactobacillus plantarum) Li-2013-01, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.7928, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101.Preservation date on July 15th, 2013.This bacterial strain feature is as follows: examine under a microscope, this bacterial strain is rod-short, and Gram's staining is positive, and boundless hair, does not produce gemma; On solid medium, this bacterium bacterium colony is white, smooth surface, and densification, form is circular, edge is more neat.Physicochemical characteristics is: catalase (-), and gelatin liquefaction (-), indoles experiment (+), motility (-), fermentation gas (-), nitrite reduction (-), fermentation gas (-), produces hydrogen sulfide gas (-), growth (+) in pH4.5MRS culture medium.
Lactobacillus plantarum of the present invention adopts following flow process to carry out seed selection:
Original sieve again → mitotic stability of bacterial classification → test tube activation → dithyl sulfate (DES) mutagenesis → dull and stereotyped primary dcreening operation → nitrosoguanidine (NTG) mutagenesis → dull and stereotyped primary dcreening operation → shaking flask test of setting out.
The original bacterial classification that sets out is CICC20242, is purchased from Chinese industrial microorganism fungus kind preservation administrative center.
Original strain of the present invention is in xylan culture medium, and the output of lactic acid is 12.5g/L.In order to improve its lactic acid production, adopt successively DES and NTG to carry out mutagenesis to this bacterial classification, mutagenesis adopts MRS calcium carbonate flat board to carry out primary dcreening operation, then adopt 500mL shake flask fermentation, biosensor analysis instrument carries out multiple sieve to Producing Strain, the lactobacillus plantarum strain that seed selection is good, the experiment of then going down to posterity, evaluates its genetic stability.
Bacterial strain CGMCC No.7928 genetic stability result shows: through continuous passage ten times, property indices is all more stable, and heredity is better, and proterties is not replied, the object bacterial strain therefore bacterial strain CGMCC No.7928 being obtained as seed selection.
Object bacterial strain CGMCC No.7928 is done to the experiment of 10L fermentation tank, and result shows: after fermentation 72h, take xylan as carbon source, the lactic acid concn of Lactobacillus plantarum CGMCC No.7928 can reach 57g/L, has improved 356% compared with starting strain.
Object bacterial strain CGMCC No.7928 is done to the experiment of 10L fermentation tank, and result shows: after fermentation 72h, take glucose as carbon source, the lactic acid concn of Lactobacillus plantarum CGMCC No.7928 can reach 68g/L.
Detailed process is as follows:
Culture medium:
Liquid MRS xylan culture medium (beef extract 2g, peptone 10g, yeast extract 5g, xylan 20g, sodium acetate 5g, ammonium citrate 2g, dipotassium hydrogen phosphate 2g, epsom salt 0.2g, seven water manganese sulfate 0.05g, after dissolving one by one, running water constant volume 1000mL, regulates pH7.0-7.2); MRS xylan screening solid medium (beef extract 2g, peptone 10g, yeast extract 5g, xylan 90g, sodium acetate 5g, ammonium citrate 2g, dipotassium hydrogen phosphate 2g, epsom salt 0.2g, seven water manganese sulfate 0.05g, after dissolving one by one, running water constant volume 1000mL, regulate pH7.0-7.2, add 20g agar).
Dithyl sulfate (DES) mutagenic and breeding:
On super-clean bench, get Lactobacillus plantarum one ring on test tube slant, access is equipped with in the 250mL triangular flask of 50mL liquid MRS xylan culture medium, and 200rpm cultivates 12h left and right for 40 ℃, makes thalline in logarithmic growth in earlier stage.
Get 5mL bacterium liquid, the centrifugal 10min of 5000rpm collects thalline, with physiological saline washing 2 times.
Be diluted to 107/mL bacteria suspension with pH7.0 phosphate buffer.
The kaliumphosphate buffer, 8mL bacteria suspension, 0.4mL DES of getting 32mL pH7.0 fully mixes at the 150mL triangular flask of putting in advance rotor, and making DES ultimate density is 1%(v/v).
In 30 ℃ of shaking tables, 150rpm reaction 30min, gets 1mL mixed liquor, adds 0.5mL25%Na
2s2O
3solution stopped reaction.
Dilution spread is in the MRS xylan screening solid medium plate containing 90g/L xylan.At the bacterial strain of 40 ℃ of cultivations picking transparent circle/colony diameter maximum after 2～3 days, label is DES bacterium.
Nitrosoguanidine mutagenesis:
On super-clean bench, get Lactobacillus plantarum DES mono-ring on test tube slant, access is equipped with in the 250mL triangular flask of 50mL liquid MRS xylan culture medium, and 200rpm cultivates 12h left and right for 40 ℃, makes thalline in logarithmic growth in earlier stage.
Get the centrifugal 10min of 5mL bacterium liquid 5000rpm and collect thalline, with physiological saline washing 2 times.
Be diluted to 107/mL bacteria suspension with pH6.0 phosphate buffer.
Get 10mL bacteria suspension and be transferred in 100mL triangular flask, add the NTG of 10mg, being mixed with final concentration is the NTG solution of 10mg/mL, and adds 4-5 to drip acetone, is beneficial to NTG and dissolves.
200rpm oscillating reactions 30min at 30 ℃, the centrifugal 10min of 5000rpm collects thalline, by SPSS washing several, stopped reaction.
Suitably dilution is coated with, and gets last dilution bacterium liquid 0.2mL, and dilution spread is in the MRS xylan screening solid medium plate containing 90g/L xylan.150 of 40 ℃ of cultivation bacterial strains that after 2～3 days, picking transparent circle/colony diameter is larger.
Shaking flask is sieved again:
On super-clean bench, get respectively Lactobacillus plantarum one ring on each test tube slant, access is equipped with in the 250mL triangular flask of 50mL liquid MRS xylan culture medium, and 200rpm cultivates 3-4 days, detects concentration of glucose and Pfansteihl change in concentration every day for 40 ℃.After fermentation ends, relatively the xylan wear rate of 150 strain bacterial classifications and lactic acid produce conversion ratio and the heteroacid content of speed, lactic acid.
Selection xylan metabolic rate is fast, lactic acid concn is high, conversion ratio is high and the poor bacterial classification of heteroacid is final bacterial classification, called after Li bacterium.
Genetic stability test:
Li-2013-01 bacterial strain is gone down to posterity for continuous ten times on inclined-plane, and the method for sieving again by shaking flask detects the fermentation situation after at every turn going down to posterity.Experiment discovery is gone down to posterity for continuous ten times on inclined-plane, and this bacterial classification proterties does not have significant change, and property indices is all normal, illustrates that the genetic stability of this bacterial classification is stronger.
Embodiment 1
A kind of feed addictive of layer chicken, parts by weight consist of: 50 parts of corns, 8 parts, rice bran, 6 parts of wheat brans, 6 parts of peanut meals, 2 parts of vegetable oil, 0.2 part of salt, 0.1 part of calcium constituent additive, 2 parts, amino acid, 0.05 part of complex enzyme formulation, 0.012 part of phytase, 0.12 part of Choline Chloride, 50 parts of vitamin e1s, 5 parts of cordyceps, 6 parts of Tianmen Green bean noodles, 1 part of licorice powder, 8 parts of bacillus subtilis CGMCC7926 microbial inoculum cultures, 3 parts of Lactobacillus plantarum CGMCC7928 microbial inoculums, 1 part of saccharomyces cerevisiae microbial inoculum.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer.
Described licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer.
Described cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step and obtains zymotic fluid, and zymotic fluid centrifugation obtains mycelia, and mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 08%, Fructus Corni 0.7%, peptone 0.4%, soyabean protein powder 0.6%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0.
Described calcium constituent additive is calcium carbonate.
Described amino acid is lysine and methionine, and the mass ratio of the two is 1:1.
In described complex enzyme formulation, the parts by weight content of each component is as follows: 5 parts of zytases, 2 parts of mannases, protease 3 part.
Preparation method: Tianmen Green bean noodle and licorice powder after pulverizing are mixed with other raw materials according to formula rate.
Embodiment 2
A kind of feed addictive of layer chicken, parts by weight consist of: 35 parts of corns, 10 parts, rice bran, 9 parts of wheat brans, 9 parts of peanut meals, 0.5 part of vegetable oil, 0.4 part of salt, 0.2 part of calcium constituent additive, 2.6 parts, amino acid, 0.05 part of complex enzyme formulation, 0.012 part of phytase, 0.05 part of Choline Chloride, 50 parts of vitamin Es, 1 part of cordyceps, 2 parts of Tianmen Green bean noodles, 0.5 part of licorice powder, 5 parts of bacillus subtilis CGMCC7926 microbial inoculum cultures, 3 parts of Lactobacillus plantarum CGMCC7928 microbial inoculums, 1 part of saccharomyces cerevisiae microbial inoculum.
Described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer.
Described licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer.
Described cordyceps preparation method: cordyceps species obtains seed liquor through inoculation fermentation, cultivates step by step and obtains zymotic fluid, and zymotic fluid centrifugation obtains mycelia, and mycelia drying and crushing obtains mycelium powder of sinensis.
Described Cordyceps Militaris fermentation medium percentage by weight consists of: sucrose 4%, and glucose 3%, Tianmen Green bean noodle 08%, Fructus Corni 0.7%, peptone 0.4%, soyabean protein powder 0.6%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, insufficient section pure water is supplied, pH6.0.
Described calcium constituent additive is calcium monohydrogen phosphate.
Described amino acid is threonine and tryptophan, and the mass ratio of the two is 1:1.
In described complex enzyme formulation, the parts by weight content of each component is as follows: 6 parts of zytases, 2 parts of mannases, 2 parts, protease.
Preparation method: Tianmen Green bean noodle and licorice powder after pulverizing are mixed with other raw materials according to formula rate.
Embodiment 3
Test:
Tested 22 days-December 24 September in 2013 and carry out at chicken house.Control group is the common peak material of laying eggs, and test group is expected for the present invention that feeds gets rid of rejection crown material and peak in hat phase lay heavily laying hen nutrition set meal application model early stage.
Test chicken grouping: test adopts single-factor experimental design, experiment material is selected 1152 blue brown 126 age in days laying hens in sea, is divided into two groups, establishes 6 repetitions for every group, 96 chickens of each repetition;
Feed respectively control group and feed group of the present invention.
Feeding method: control group free choice feeding, normally feed according to market ordinary circumstance, not stage by stage; 16 weeks age-2% laying rate of test group, 16 tons/thousand chickens of rejection crown material of feeding, all change to 2% laying rate the peak material of laying eggs into, and during to 28 weeks age, expect early stage on the feed 8 tons peak of laying eggs of every thousand chickens.
Test temperature: adopt longitudinal ventilation, hen house temperature is controlled at 20-25 ℃.Strictly control according to the blue brown feeding and management in sea.
Testing index comprises: body weight while finishing (start and twice), feed intake, survival rate, laying rate, egg size, feedstuff-egg ratio, Egg Quality and quality of egg etc., the results are shown in Table 1, table 2 and table 3.
The impact of table 1 the present invention on laying hen expected date of childbirth production performance
The impact of table 2 the present invention on laying hen 2% laying rate-28 egg laying performance in week age
Note: the female different significant differences (P < 0.05) that represent of colleague's shoulder marking-up.
From table 1 and table 2 result of the test, we can find out, get rid of the hat phase and lay eggs peak in earlier stage, feed of the present invention has good behaviour at aspects such as egg size, laying rate, feedstuff-egg ratio, feed intake and survival rates, is all better than common material group, has absolutely proved the scientific and advanced of product of the present invention.
Claims (10)
1. a feed addictive of layer chicken, it is characterized in that, parts by weight consist of: corn 35-65 part, rice bran 5-10 part, wheat bran 4-9 part, peanut meal 5-9 part, vegetable oil 0.5-3 part, salt 0.2-0.4 part, calcium constituent additive 0.1-0.2 part, amino acid 0.4-2.6 part, 0.05 part of complex enzyme formulation, 0.012 part of phytase, Choline Chloride 0.05-0.12 part, vitamin E 50-150 part, cordyceps 1-5 part, Tianmen Green bean noodle 2-6 part, licorice powder 0.5-1 part, bacillus subtilis CGMCC7926 microbial inoculum culture 5-8 part, Lactobacillus plantarum CGMCC7928 microbial inoculum 3-6 part, saccharomyces cerevisiae microbial inoculum 1-2 part.
2. a kind of feed addictive of layer chicken according to claim 1, is characterized in that, described lucid asparagus powder, preparation method thereof is as follows: it is below 1 millimeter that lucid asparagus is crushed to particle diameter through pulverizer.
3. a kind of feed addictive of layer chicken according to claim 1, is characterized in that, described licorice powder preparation method is as follows: it is below 1 millimeter that Radix Glycyrrhizae is crushed to particle diameter through pulverizer.
4. a kind of feed addictive of layer chicken according to claim 1, it is characterized in that, it is characterized in that, parts by weight consist of: 50 parts of corns, 8 parts, rice bran, 6 parts of wheat brans, 6 parts of peanut meals, 2 parts of vegetable oil, 0.2 part of salt, 0.1 part of calcium constituent additive, 2 parts, amino acid, 0.05 part of complex enzyme formulation, 0.012 part of phytase, 0.12 part of Choline Chloride, 50 parts of vitamin e1s, 5 parts of cordyceps, 6 parts of Tianmen Green bean noodles, 1 part of licorice powder, 8 parts of bacillus subtilis CGMCC7926 microbial inoculum cultures, 3 parts of Lactobacillus plantarum CGMCC7928 microbial inoculums, 1 part of saccharomyces cerevisiae microbial inoculum.
5. a kind of feed addictive of layer chicken according to claim 1, is characterized in that, described calcium constituent additive is a kind of in calcium carbonate, calcium monohydrogen phosphate or calcium dihydrogen phosphate or two kinds.
6. a kind of feed addictive of layer chicken according to claim 1, is characterized in that, described amino acid is two or more the mixing in lysine, methionine, threonine or tryptophan.
7. a kind of feed addictive of layer chicken according to claim 1, is characterized in that, in described complex enzyme formulation, the parts by weight content of each component is as follows: zytase 3-6 part, mannase 1-2 part, protease 1-3 part; The unit of above-mentioned enzyme is as follows: zytase 20000-30000U/g, mannase 1000-40000U/g, protease 3 000-5000U/g.
8. a kind of feed addictive of layer chicken according to claim 1, it is characterized in that, described bacillus subtilis culture preparation: cultivate bacillus subtilis from inclined-plane switching, seed liquor after spreading cultivation is step by step transferred in fermentation tank, control temperature is 28-30 ℃, aerlbic culture 19-24 hour, throughput is 2.0m
3/ minute; Zymotic fluid plate-frame filtering, the culture that dry acquisition contains bacillus subtilis.
9. according to the preparation method of a kind of feed addictive of layer chicken described in claim 1-8: Tianmen Green bean noodle and licorice powder after pulverizing are mixed with other raw materials according to formula rate.
10. the application of a kind of feed addictive of layer chicken claimed in claim 1 aspect layer breeding.
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Effective date of registration: 20170911 Address after: Wan Xing Garden, Hedong District, Tianjin, 1-3-503 Co-patentee after: Tang Xueping Patentee after: Wang Hailin Address before: 211600 Jinhu health Road, Jiangsu, No. 32, No. Patentee before: Zhu Jianguo |
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| TR01 | Transfer of patent right |
Effective date of registration: 20180322 Address after: Haimen Changle Town Jiangsu city of Nantong Province on the 226100 Road No. 207 Patentee after: Haimen embroidery Industrial Design Co., Ltd. Address before: 0 Wanxing garden, Hedong District, Tianjin, Tianjin Co-patentee before: Tang Xueping Patentee before: Wang Hailin |