CN106035991A - High-protein high-calcium feedstuff prepared from fermented moringa seeds and preparation method of high-protein high-calcium feedstuff - Google Patents
High-protein high-calcium feedstuff prepared from fermented moringa seeds and preparation method of high-protein high-calcium feedstuff Download PDFInfo
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Abstract
The invention relates to a high-protein high-calcium feedstuff prepared from fermented moringa seeds and a preparation method of the high-protein high-calcium feedstuff. The high-protein high-calcium feedstuff prepared from fermented moringa seeds is characterized in that a strain stock solution which is obtained by mixing bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus fermentum proportionally is inoculated into pulverized moringa seed powder for fermentation, and after the mixture is dried, the high-protein high-calcium moringa seed feedstuff is obtained. The problem of calcium absorption and nutrient protein supplementing of laying hens and broilers can be solved by the fermented moringa seed feedstuff, and the fermented moringa seed feedstuff is a green and safe cultivation technological novel product.
Description
Technical field
The present invention relates to a kind of high protein high calcium feedstuff utilizing fermentation moringa seeds to make, be to utilize multiple-microorganism
The rich in proteins of fermentation moringa seeds making and calcareous product, belong to feed additive field.
Background technology
Calcium is one of important nutrient of laying hen and broiler stable high yield.General hen often produces 1 piece of egg about needs calcareous 4
~ 4.4 grams.If laying hen takes in calcareous deficiency, not only easily there is rickets in chicken group, also can affect eggshell quality and laying rate.And
When in daily ration, calcium content is more than 4%, will cause urate at chicken body accumulation, cause dyspepsia to cause chicken to have loose bowels or
Gout occurs.And also feedstuff palatability can be made to be deteriorated, make chicken feed intake and egg production reduce, have a strong impact on its productivity
Can, reduce raise benefit.Therefore, want to solve this contradictory problems, be necessary for taking science to replenish the calcium.
Sajina and China Ganoderma, U.S.'s Radix Panacis Quinquefolii claim " world's three essentials-essence ".Moringa is also known as drumstick tree, Moringaceae Moringa
Platymiscium, is perennial tropical trees, originates in India.Moringa seeds is the seed of Moringa tree, at food, medicine, keeps healthy, makes up
The fields such as product suffer from good using value.Moringa rich in vitamin, protein, fiber, possibly together with mineral element (calcium,
Magnesium, phosphorus, potassium, sodium, sulfur) and trace element (zinc, copper, ferrum, manganese, selenium).Moringa seeds protein content is 36.2% ~ 38.7%, and calcium contains
Amount is 95 ~ 135.3mg/100g, is 4 times of calcium content in milk.
In applying for a patent CN 105053566 A bicycle beam wood seed Pepsin feed additive and preparation method thereof
Disclose will pulverize after the bicycle beam wood seed dregs of rice, leaf of Moringa powder and moringa seeds dregs of rice powder through bacillus acidophilus's liquid, bifidobacterium bifidum liquid,
The complex microorganism preparations 28 DEG C fermentation 15 of animal bifidobacteria liquid, Candida utilis liquid and thermophilus bacterium solution composition
My god, the Pepsin feed additive that obtains of cold air drying.This patented method can be by the bicycle beam wood seed dregs of rice, leaf of Moringa powder, moringa seeds
Crude protein, the polysaccharide etc. that are difficult in the dregs of rice etc. be absorbed by animal resolve into the little molecular nutrition material easily absorbed by animal.
But the microorganism used by fermentation is based on lactic acid bacteria and Candida utilis, to the bicycle beam wood seed dregs of rice, leaf of Moringa powder and peppery
The capacity of decomposition of wood seed dregs of rice powder is limited, thus fermentation time was up to 15 days, and energy consumption is higher.
Summary of the invention
It is an object of the invention to provide a kind of high protein high calcium feedstuff utilizing fermentation moringa seeds to make, can solve
The problem that laying hen and the calcium absorption of broiler and nutrient protein are supplemented.
For realizing object above, the technical scheme is that and provide a kind of high protein utilizing fermentation moringa seeds to make high
Calcium feedstuff and preparation method thereof, it is characterised in that:
Step 1, is crushed to more than 200 mesh by moringa seeds, obtains moringa seeds powder；
Step 2, the strain after bacillus subtilis, saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus fermenti are mixed in proportion
Stock solution accesses step 1 moringa seeds powder and ferments；
Step 3, i.e. obtains the moringa seeds feedstuff of high protein high calcium after drying the moringa seeds powder after fermentation.
Bacillus subtilis, saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus fermenti warp before the use in above-mentioned steps 2
Cultivation below crossing:
Preferably, the cultivation of described bacillus subtilis is for transfer bacillus subtilis in nutrient broth fluid medium
Carrying out shaken cultivation, the temperature range of its shaken cultivation is preferably 28 ~ 40 DEG C, more preferably 32 ~ 37 DEG C, turning of its shaken cultivation
Number ranges preferably from 150-250rpm, more preferably 180-220rpm, and the time of its shaken cultivation is preferably 24-72h, more preferably
For 36-48h, take out after culture medium muddiness, be then placed in 2-4 DEG C of Refrigerator store stand-by.
Preferably, described Lactobacillus plantarum and the cultivation of Lactobacillus fermenti are for turn Lactobacillus plantarum and Lactobacillus fermenti
Accessing in 5% molasses fluid medium and cultivate, its temperature range cultivated is preferably 28-40 DEG C, more preferably 30-35 DEG C,
Its incubation time ranges preferably from 24-72h, more preferably 36-48h, takes out, be then placed in 2-4 DEG C of ice after culture medium muddiness
Case preserves stand-by.
Preferably, the cultivation of described saccharomyces cerevisiae is for transfer saccharomyces cerevisiae into potato glucose liquid culture
Carrying out shaken cultivation in base, the temperature range of its shaken cultivation is preferably 26-35 DEG C, more preferably 28-32 DEG C, its shaken cultivation
Range of revolution be preferably 150-250rpm, more preferably 180-220rpm, the time of its shaken cultivation is preferably 24-72h, more
It is preferably 36-48h, takes out after culture medium muddiness, be then placed in 2-4 DEG C of Refrigerator store stand-by.
Described bacillus subtilis (Bacillus subtilis) is preferably the bacterium that deposit number is ACCC No:10619
Kind.
Described saccharomyces cerevisiae (Saccharomyces cerevisiae) be preferably deposit number be ACCC No:
The strain of 20065.
It is ACCC No:11118 that described Lactobacillus plantarum (Lactobacillus plantarum) is preferably deposit number
Strain.
It is ACCC No:05478 that described Lactobacillus fermenti (Lactobacillus fermentum) is preferably deposit number
Strain.
The part by weight of bacillus subtilis, saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus fermenti in above-mentioned steps 2
For, (5-15): (20-40): (20-35): (20-35), obtain strain stock solution after being mixed in proportion.
The part by weight of the strain stock solution described in above-mentioned steps 2, moringa seeds powder, molasses and water mixing is (5-10):
(50-75): (1-5): (20-45).
In sweat described in above-mentioned steps 2, fermentation temperature scope is 25-40 DEG C, preferably 30-37 DEG C.
In sweat described in above-mentioned steps 2, fermentation time is 120-240 hour, preferably 160-240 hour.
In above-mentioned steps 3, the moisture after the moringa seeds feedstuff after fermentation is dried is 5-13%, preferably 7-10%.
Compared with existing feed additive, the high-calcium, high-protein feedstuff of fermentation moringa seeds of the present invention is not only
Combine calcium and protein containing the organic acid easily absorbed, possibly together with the metabolite of multiple probiotic bacteria and laying hen and broiler are had
The vitamin of benefit and mineral composition, can reduce the feedstuff-meat ratio of laying hen and broiler, improves the content of the protein of meat egg, reduces
Production cost.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Embodiment one
Making the high-calcium, high-protein feedstuff of fermentation moringa seeds, the strain that the present embodiment is selected is:
Bacillus subtilis (Bacillus subtilis) is preferably the strain that deposit number is ACCC No:10619；
Saccharomyces cerevisiae (Saccharomyces cerevisiae) be deposit number be the strain of ACCC No:20065；
Lactobacillus plantarum (Lactobacillus plantarum) is preferably the strain that deposit number is ACCC No:11118；
Lactobacillus fermenti (Lactobacillus fermentum) is preferably the strain that deposit number is ACCC No:05478；
The manufacture method of the high-calcium, high-protein feedstuff of fermentation moringa seeds, its step includes:
Step 1, is crushed to more than 200 mesh by moringa seeds, obtains moringa seeds powder；
Step 2, first cultivates bacillus subtilis, saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus fermenti as seed liquor:
Being transferred by above-mentioned bacillus subtilis in nutrient broth fluid medium at 37 DEG C, revolution is vibration training under 220rpm
Support 36 hours, take out after culture medium muddiness, be then placed in 2-4 DEG C of Refrigerator store stand-by；
Above-mentioned Lactobacillus plantarum and Lactobacillus fermenti are transferred in the conical flask of 5% molasses fluid medium, 32 DEG C of cultivations
48 hours, take out after culture medium muddiness, be then placed in 2-4 DEG C of Refrigerator store stand-by；
Being transferred by above-mentioned saccharomyces cerevisiae in the conical flask of potato dextrose broth, at 28 DEG C, revolution is
Shaken cultivation 48 hours under 220rpm, take out after culture medium muddiness, are then placed in 2-4 DEG C of Refrigerator store stand-by.
The strain of above-mentioned cultivation is mixed to prepare strain stock solution, wherein, bacillus subtilis: saccharomyces cerevisiae: plant breast
Bacillus: the part by weight of Lactobacillus fermenti is 10:20:35:35.
By the moringa seeds powder strain stock solution of the strain stock solution weighed above and step 1, moringa seeds powder, molasses, moisture according to
Part by weight is to ferment 240 hours at 30 DEG C after 5:65:5:25 mixes；
Step 3, dries the moringa seeds powder after fermentation to moisture content 7%, obtains the moringa seeds feedstuff of high protein high calcium.
Embodiment two
Selecting the 25 female broiler of age in days that 200 health, body weight are close, be randomly divided into 2 groups, often group 100, experimental group is fed
Food normal feedstuff and 1%(w/w) the moringa seeds feed additive of high protein high calcium, matched group feeding normal feedstuff, persistently feed 20
My god, the production performance of broiler is as shown in table 1.
The moringa seeds feed additive of table 1 high protein high calcium is on broiler starting weight, end weight, daily gain and the impact of feedstuff-meat ratio
By table 1 it can be seen that feedstuff adds the high-calcium, high-protein feedstuff of the fermentation moringa seeds of the present invention to improving feedstuff
The production performance of utilization rate, raising laying hen and broiler has the biggest facilitation, and the high-calcium, high-protein adding fermentation moringa seeds is raised
The experimental group of material raw material is compared feedstuff-meat ratio with the matched group of feeding normal feedstuff and is decreased obviously, and the feed intake of every day substantially drops
Low, average daily gain becomes big, substantially increases the utilization rate of feedstuff, reduces production cost.
Being described in detail the specific embodiment of the present invention above, but it is only used as example, the present invention is not limiting as
In particular embodiments described above.To those skilled in the art, any equivalent modifications that this practicality is carried out and replacing
In generation, is the most all among scope of the invention.Therefore, the equalization made without departing from the spirit and scope of the invention converts and repaiies
Change, all should contain within the scope of the invention.
Claims (8)
1. the manufacture method of the high protein high calcium feedstuff utilizing fermentation moringa seeds to make, it is characterised in that:
Step 1, is crushed to more than 200 mesh by moringa seeds, obtains moringa seeds powder；
Step 2, the strain after bacillus subtilis, saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus fermenti are mixed in proportion
Stock solution accesses step 1 moringa seeds powder and ferments；
Step 3, i.e. obtains the moringa seeds feedstuff of high protein high calcium after drying the moringa seeds powder after fermentation.
Bacillus subtilis, saccharomyces cerevisiae, Lactobacillus plantarum and fermentation milk bar in step 2 the most according to claim 1
Bacterium is before the use through following cultivation:
Preferably, the cultivation of described bacillus subtilis is for transfer bacillus subtilis in nutrient broth fluid medium
Carrying out shaken cultivation, the temperature range of its shaken cultivation is preferably 28 ~ 40 DEG C, more preferably 32 ~ 37 DEG C, turning of its shaken cultivation
Number ranges preferably from 150-250rpm, more preferably 180-220rpm, and the time of its shaken cultivation is preferably 24-72h, more preferably
For 36-48h, take out after culture medium muddiness, be then placed in 2-4 DEG C of Refrigerator store stand-by；Preferably, described Lactobacillus plantarum
Cultivation with Lactobacillus fermenti for 5% molasses fluid medium that Lactobacillus plantarum and Lactobacillus fermenti transferred is cultivated,
Its temperature range cultivated is preferably 28-40 DEG C, and more preferably 30-35 DEG C, its incubation time ranges preferably from 24-72h, more excellent
Elect 36-48h as, take out after culture medium muddiness, be then placed in 2-4 DEG C of Refrigerator store stand-by；Preferably, described saccharomyces cerevisiae
The cultivation of bacterium for potato dextrose broth of being transferred by saccharomyces cerevisiae carries out shaken cultivation, its shaken cultivation
Temperature range is preferably 26-35 DEG C, more preferably 28-32 DEG C, and the range of revolution of its shaken cultivation is preferably 150-250rpm, more
Being preferably 180-220rpm, the time of its shaken cultivation is preferably 24-72h, and more preferably 36-48h takes after culture medium muddiness
Go out, be then placed in 2-4 DEG C of Refrigerator store stand-by.
Bacillus subtilis, saccharomyces cerevisiae, Lactobacillus plantarum and fermentation milk in step 2 the most according to claim 1
The part by weight of bacillus is, (5-15): (20-40): (20-35): (20-35), obtains strain stock solution after being mixed in proportion.
Strain stock solution described in step 2 the most according to claim 1, moringa seeds powder, molasses and the weight ratio of water mixing
Example is (5-10): (50-75): (1-5): (20-45).
In sweat described in step 2 the most according to claim 1, fermentation temperature scope is 25-40 DEG C, is preferably
30-37℃。
In sweat described in step 2 the most according to claim 1, fermentation time is 120-240 hour, is preferably
160-240 hour.
In step 3 the most according to claim 1, the moisture after the moringa seeds feedstuff after fermentation is dried is 5-13%, excellent
Elect 7-10% as.
8. the high protein high calcium feedstuff that a production method as claimed in claim 1 produces.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107549817A (en) * | 2017-08-21 | 2018-01-09 | 云南农业大学 | A kind of natural organic calcium of Moringa and preparation method thereof |
| CN109007295A (en) * | 2018-06-28 | 2018-12-18 | 中国热带农业科学院农产品加工研究所 | A kind of Moringa feed addictive, preparation method and purposes |
| CN109548954A (en) * | 2018-12-06 | 2019-04-02 | 华南农业大学 | A kind of Moringa cauline leaf powder process for solid state fermentation |
| CN109700009A (en) * | 2018-12-29 | 2019-05-03 | 江瀚生物科技（上海）有限公司 | A kind of natural nutrient and preparation method thereof for repairing osteoporosis crowd bone function |
| CN111000023A (en) * | 2019-11-25 | 2020-04-14 | 金银卡(广州)生物科技股份有限公司 | Microbial high-cellulose fermented feed for livestock |
| CN111000025A (en) * | 2019-11-28 | 2020-04-14 | 金银卡(广州)生物科技股份有限公司 | Microbial compound additive preparation for poultry feed |
| CN111011581A (en) * | 2019-11-28 | 2020-04-17 | 金银卡(广州)生物科技股份有限公司 | Process for producing microbial composite preparation for poultry |
| CN112690373A (en) * | 2020-12-25 | 2021-04-23 | 福建傲农生物科技集团股份有限公司 | Composition for preventing white spot syndrome of prawns as well as preparation method and application thereof |
| CN115944061A (en) * | 2022-12-23 | 2023-04-11 | 山东劲牛集团股份有限公司 | Preparation method of quick-acting calcium type fermented high-calcium vegetable powder |
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| CN109007295A (en) * | 2018-06-28 | 2018-12-18 | 中国热带农业科学院农产品加工研究所 | A kind of Moringa feed addictive, preparation method and purposes |
| CN109548954A (en) * | 2018-12-06 | 2019-04-02 | 华南农业大学 | A kind of Moringa cauline leaf powder process for solid state fermentation |
| CN109700009A (en) * | 2018-12-29 | 2019-05-03 | 江瀚生物科技（上海）有限公司 | A kind of natural nutrient and preparation method thereof for repairing osteoporosis crowd bone function |
| CN111000023A (en) * | 2019-11-25 | 2020-04-14 | 金银卡(广州)生物科技股份有限公司 | Microbial high-cellulose fermented feed for livestock |
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| CN111011581A (en) * | 2019-11-28 | 2020-04-17 | 金银卡(广州)生物科技股份有限公司 | Process for producing microbial composite preparation for poultry |
| CN112690373A (en) * | 2020-12-25 | 2021-04-23 | 福建傲农生物科技集团股份有限公司 | Composition for preventing white spot syndrome of prawns as well as preparation method and application thereof |
| CN115944061A (en) * | 2022-12-23 | 2023-04-11 | 山东劲牛集团股份有限公司 | Preparation method of quick-acting calcium type fermented high-calcium vegetable powder |
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Application publication date: 20161026 |