CN103718695B - Method for growing seedlings of cephalotaxus oliveri seeds - Google Patents
Method for growing seedlings of cephalotaxus oliveri seeds Download PDFInfo
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- CN103718695B CN103718695B CN201310735024.7A CN201310735024A CN103718695B CN 103718695 B CN103718695 B CN 103718695B CN 201310735024 A CN201310735024 A CN 201310735024A CN 103718695 B CN103718695 B CN 103718695B
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Abstract
The invention relates to a method for growing seedlings of cephalotaxus oliveri seeds. The method specifically comprises the following steps: selecting mature cephalotaxus oliveri seeds of which the episperm is grey and slightly claret-colored; accumulating the seeds for 5 days, turning and accumulating for 5 days again, kneading to remove the episperm, cleaning by using clear water, and drying; removing moldy seeds; arranging the selected seeds on an electric field treatment rack, starting an electric field generator with the voltage of 10 kilovolt, and treating 8 minutes; accumulating and storing the seeds according to a ratio 3:1 of the sand to the seeds by using the sterilized clear water sand for 6 months; screening the seeds after beginning of spring, soaking the seeds for 2 hours by using a Guoguang rooting agent and a carbendazim mixing solution, taking the seeds out and drying for later use; laying a black plastic film on the finished seed bed, seeding the seeds in soil, arching through bamboo skin and covering the film; and checking the damage caused by rats and emergence conditions in the period of waiting for emergence. By adoption of the method for growing seedlings of the seeds, the emergence rate of the seeds is about 40 percent higher than the emergence rate of seeds which are not subjected to electromagnetic field treatment, the survival rate of the seedlings is extremely high, and the method is suitable for large-scale planting needs.
Description
Technical field
The present invention relates to a kind of seedling-cultivating method of Cephalotaxus oliveri seed.
Background technology
Cephalotaxus oliveri is a kind under Cephalotaxaceae Cephalotaxus, national second class protection vulnerable species.Cephalotaxus oliveri is because the double-edged fine-toothed comb of the picture China of its leaf arrangement, obtains bamboo comb Cephalotaxus oliveri by name; From Cephalotaxus oliveri, can obtain altogether four kinds of alkaloids, one is high thorn ketone alkaloid, not special active; Another three kinds is caephalotaxus sinensis bases, and harringtonine wherein and homoharringtonine have and do leukemic activity, and cephalotaxine not significant biologically active itself.Therefore the medical value of bamboo comb Cephalotaxus oliveri is quite high.
But the germination and growth rate of bamboo comb Cephalotaxus oliveri seed is very low under field conditions (factors), few by the seedling of Seed Development, natural regeneration difficulty.Add artificial felling, the interference of mankind's activity makes its living environment be damaged, result in the rare and in imminent danger of Cephalotaxus oliveri.
Summary of the invention
The object of this invention is to provide the seedling-cultivating method of the higher cephalotaxus oliveri seed of a kind of bud ratio.
oneplant the seedling-cultivating method of Cephalotaxus oliveri seed, its concrete steps are:
(1) seed collection: select the gloomy Cephalotaxus oliveri seed being about with aubergine maturation of exosper;
(2) seed treatment; Gathered Cephalotaxus oliveri seed is piled up 5 days, and turning piles up 5 days again, and exosper is removed in rubbing, and clean water is clean, dries; Remove the seed that goes rotten, obtain without mildew and rot, the hard bright seed of free from admixture, full seed, shell;
(3) electric field treatment: the seed of select is placed in threshold electric field generator on electric field treatment frame, voltage 10 kilovolts, processes 8 minutes; Storage is piled up 6 months by the husky ratio in the husky 3:1 of kind of the clear water after sterilization;
(4) soak: after the beginning of spring, seed is sifted out, invade bubble with state's light root-growing agent and carbendazim mixed liquor and pull out after 2 hours and dry, for subsequent use;
(5) sow: black mulch film is spread in whole good seedbed, and punch by 20*30 centimetre of nest, line-spacing spillikin, be sowed at by seed in hole, sprinkle fine earth, thin bamboo strips used for weaving reaches arch cover film;
(6) seedbed management: check with or without the plague of rats and seedbed moisture situation between the wait seeding stage, supplements moisture content in time;
(7) emerge: treat seed sprouting, record is emerged highly and emergence rate.
Adopt seed seedling-raising method of the present invention, the emergence rate of seed is higher by about 40% than the emergence rate without electromagnetic field process, and the survival rate of seedling is high, is applicable to very much the needs of implant mass.
Embodiment
Cephalotaxus oliveri is a kind of purposes rare species very widely, and seed, branch, leaf containing various plants alkali, have desinsection, moistening lung, treatment hemorrhoid, disappear the effect such as long-pending, cures mainly parasites disease due to noxious agents produced by various parasites, cough and infantile malnutrition.Branch, leaf can control malignant tumour, has good therapeutic effect to lymphosarcoma, lung are stubborn etc.Since the sixties in 20th century, the alkaloid that vegetalization scholar extracts from the branches and leaves, bark of cephalotaxus oliveri, through clinical testing, prove a kind of novel PTS, to the non-lymphoid leukaemia for the treatment of human body, particularly acute myeloblastic leukemia and monokaryon type chronic myeloid leukemia have good curative effect, have anti-cancer, the special medicinal efficacy such as anticancer, for cancer.
But due to the natural breed difficulty of Cephalotaxus oliveri, cause its output not catch up with the needs in market.The present invention, according to the characteristic of Cephalotaxus oliveri, develops the seedling-cultivating method that a kind of emergence rate is higher.
oneplant the seedling-cultivating method of Cephalotaxus oliveri seed:
(1) seed collection: on September 12nd, 2013, collecting location: great Gan town, Dao Zhen county, collecting quantity: about 1200, one kilogram of fresh seed, choosing adopts that exosper is gloomy is about with aubergine and mature seed;
(2) seed treatment; Gathered Cephalotaxus oliveri seed is piled up 5 days, and turning piles up 5 days again, and exosper is removed in rubbing, and clean water is clean, dries; Remove the seed that goes rotten, obtain without mildew and rot, hard 1168, the bright seed of free from admixture, full seed, shell;
(3) electric field treatment: the seed of select is placed in threshold electric field generator on electric field treatment frame, voltage 10 kilovolts, processes 8 minutes; Storage is piled up 6 months by the husky ratio in the husky 3:1 of kind of the clear water after sterilization;
(4) soak: on March 28th, 3013, after seed is sifted out, invade bubble after 2 hours with state's light root-growing agent and carbendazim mixed liquor, pull out and dry, for subsequent use;
(5) sow: black mulch film is spread in whole good seedbed, and punch by 20*30 centimetre of nest, line-spacing spillikin, be sowed at by seed in hole, sprinkle fine earth, thin bamboo strips used for weaving reaches arch cover film;
(6) seedbed management: check with or without the plague of rats and seedbed moisture situation between the wait seeding stage, supplements moisture content in time;
(7) emerge: May 22 in 2013 day entry: average 4.8 centimetres of height of seedling, 1096 strains of emerging, emergence rate 91.3%.
Following table is adopt electromagnetic field process seed and do not adopt the correlation index of electromagnetic field process seed to contrast:
| Title | Seed (grain) | Cycle (moon) | Height of seedling (centimetre) | Emerge and count (strain) | Emergence rate (%) |
| Do not adopt electromagnetic field process | １２００ | ８ | ３.５ | ６３２ | ５５% |
| Adopt electromagnetic field process | １２００ | ８ | ４.８ | １０９６ | ９１.３ |
Can find out from upper table, adopt the seed of electromagnetic field process, its bud ratio is far above the seed without electromagnetic field process.
Claims (1)
1. the seedling-cultivating method of kind of Cephalotaxus oliveri seed, is characterized in that: the concrete steps of described seedling-cultivating method are:
(1) seed collection: select the gloomy Cephalotaxus oliveri seed being about with aubergine maturation of exosper;
(2) seed treatment: gathered Cephalotaxus oliveri seed is piled up 5 days, and turning piles up 5 days again, exosper is removed in rubbing, and clean water is clean, dries; Remove the seed that goes rotten, obtain without mildew and rot, the hard bright seed of free from admixture, full seed, shell;
(3) electric field treatment: the seed of select is placed in threshold electric field generator on electric field treatment frame, voltage 10 kilovolts, processes 8 minutes; Storage is piled up 6 months by the husky ratio in the husky 3:1 of kind of the clear water after sterilization;
(4) soak: after the beginning of spring, seed is sifted out, invade bubble with state's light root-growing agent and carbendazim mixed liquor and pull out after 2 hours and dry, for subsequent use;
(5) sow: black mulch film is spread in whole good seedbed, and punch by 20*30 centimetre of nest, line-spacing spillikin, be sowed at by seed in hole, sprinkle fine earth, thin bamboo strips used for weaving reaches arch cover film;
(6) seedbed management: check with or without the plague of rats and seedbed moisture situation between the wait seeding stage, supplements moisture content in time;
(7) emerge: treat seed sprouting, record is emerged highly and emergence rate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310735024.7A CN103718695B (en) | 2013-12-27 | 2013-12-27 | Method for growing seedlings of cephalotaxus oliveri seeds |
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| CN201310735024.7A CN103718695B (en) | 2013-12-27 | 2013-12-27 | Method for growing seedlings of cephalotaxus oliveri seeds |
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| CN103718695A CN103718695A (en) | 2014-04-16 |
| CN103718695B true CN103718695B (en) | 2015-01-07 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284537A (en) * | 2015-11-06 | 2016-02-03 | 贵州天豪民族药业有限公司 | Seedling method for cephalotaxus fortunei seeds |
| CN105875160A (en) * | 2016-05-04 | 2016-08-24 | 枞阳县熊天然生态农业有限公司 | Photinia fraseri seedling culture method |
| CN108668558A (en) * | 2018-04-19 | 2018-10-19 | 边学静 | A method of abolishing Cephalotaxus mannii seed dormancy |
| CN109526516A (en) * | 2018-12-13 | 2019-03-29 | 怒江景天药业有限公司 | A kind of high mountain cepehalotaxus fortunei seedling breeding method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1209057A1 (en) * | 1984-07-04 | 1986-02-07 | Московский Ордена Трудового Красного Знамени Институт Инженеров Сельскохозяйственного Производства Им.В.П.Горячкина | Arrangement for preparing seeds for sowing |
| CN102823357A (en) * | 2012-09-21 | 2012-12-19 | 南京绿宙薄壳山核桃科技有限公司 | Method for accelerating germination of carya illinoensis seeds in late winter and early spring |
| CN103168599A (en) * | 2013-04-07 | 2013-06-26 | 道真仡佬族苗族自治县谷森科技农业开发有限责任公司 | Cephalotaxus fortune cottage seedling raising method |
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2013
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1209057A1 (en) * | 1984-07-04 | 1986-02-07 | Московский Ордена Трудового Красного Знамени Институт Инженеров Сельскохозяйственного Производства Им.В.П.Горячкина | Arrangement for preparing seeds for sowing |
| CN102823357A (en) * | 2012-09-21 | 2012-12-19 | 南京绿宙薄壳山核桃科技有限公司 | Method for accelerating germination of carya illinoensis seeds in late winter and early spring |
| CN103168599A (en) * | 2013-04-07 | 2013-06-26 | 道真仡佬族苗族自治县谷森科技农业开发有限责任公司 | Cephalotaxus fortune cottage seedling raising method |
Non-Patent Citations (3)
| Title |
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| 曹基武,刘春林.篦子三尖杉生物学特性和繁殖技术.《林业科技开发》.2005,第19卷(第6期),第63-65页. * |
| 篦子三尖杉种子育苗技术研究;戴晓勇 等;《种子》;20120831;第31卷(第8期);第122-125页 * |
| 篦子三尖杉育苗技术;吴朝斌 等;《林业实用技术》;20071231(第8期);第22-23页 * |
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