CN103718695A - Method for growing seedlings of cephalotaxus oliveri seeds - Google Patents
Method for growing seedlings of cephalotaxus oliveri seeds Download PDFInfo
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- CN103718695A CN103718695A CN201310735024.7A CN201310735024A CN103718695A CN 103718695 A CN103718695 A CN 103718695A CN 201310735024 A CN201310735024 A CN 201310735024A CN 103718695 A CN103718695 A CN 103718695A
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Abstract
The invention relates to a method for growing seedlings of cephalotaxus oliveri seeds. The method specifically comprises the following steps: selecting mature cephalotaxus oliveri seeds of which the episperm is grey and slightly claret-colored; accumulating the seeds for 5 days, turning and accumulating for 5 days again, kneading to remove the episperm, cleaning by using clear water, and drying; removing moldy seeds; arranging the selected seeds on an electric field treatment rack, starting an electric field generator with the voltage of 10 kilovolt, and treating 8 minutes; accumulating and storing the seeds according to a ratio 3:1 of the sand to the seeds by using the sterilized clear water sand for 6 months; screening the seeds after beginning of spring, soaking the seeds for 2 hours by using a Guoguang rooting agent and a carbendazim mixing solution, taking the seeds out and drying for later use; laying a black plastic film on the finished seed bed, seeding the seeds in soil, arching through bamboo skin and covering the film; and checking the damage caused by rats and emergence conditions in the period of waiting for emergence. By adoption of the method for growing seedlings of the seeds, the emergence rate of the seeds is about 40 percent higher than the emergence rate of seeds which are not subjected to electromagnetic field treatment, the survival rate of the seedlings is extremely high, and the method is suitable for large-scale planting needs.
Description
Technical field
The present invention relates to a kind of seedling-cultivating method of Cephalotaxus oliveri seed.
Background technology
Cephalotaxus oliveri is a kind under Cephalotaxaceae Cephalotaxus, national second class protection vulnerable species.Cephalotaxus oliveri is because the Chinese double-edged fine-toothed comb of picture that its leaf is arranged must be called bamboo comb Cephalotaxus oliveri; From Cephalotaxus oliveri, can obtain altogether four kinds of alkaloids, one is high thorn ketone alkaloid, not special active; Another three kinds is caephalotaxus sinensis bases, and harringtonine wherein and homoharringtonine have and do leukemic activity, and cephalotaxine itself does not have significant biologically active.Therefore the medical value of bamboo comb Cephalotaxus oliveri is quite high.
But the germination and growth rate of bamboo comb Cephalotaxus oliveri seed is very low under field conditions (factors), few by the seedling of Seed Development, natural regeneration difficulty.The interference of adding artificial felling, mankind's activity is damaged its living environment, has caused the rare and in imminent danger of Cephalotaxus oliveri.
Summary of the invention
The object of this invention is to provide the seedling-cultivating method of the cephalotaxus oliveri seed that a kind of bud ratio is higher.
onethe seedling-cultivating method of planting Cephalotaxus oliveri seed, its concrete steps are:
(1) seed collection: select the gloomy approximately Cephalotaxus oliveri seed with aubergine maturation of exosper;
(2) seed treatment; Gathered Cephalotaxus oliveri seed is piled up 5 days, and turning is piled up 5 days again, and exosper is removed in rubbing, and clear water cleans up, and dries; Remove the seed that goes rotten, obtain without mildew and rot, free from admixture, full seed, the hard bright seed of shell;
(3) electric field treatment: the seed of select is placed on electric field treatment frame and opens field generator, and 10 kilovolts of voltages, process 8 minutes; With the clear water after sterilization is husky, in the ratio of the husky 3:1 of kind, pile up storage 6 months;
(4) soak: after the beginning of spring, seed is sifted out, with state's light root-growing agent and carbendazim mixed liquor, invade bubble and pull out and dry after 2 hours, standby;
(5) sowing: by whole good seedbed spread black mulch film, by 20*30 centimetre of nest, line-spacing, with spillikin, punch, seed is sowed in hole, sprinkle fine earth, thin bamboo strips used for weaving reaches arch cover film;
(6) seedbed management: waiting for that between the seeding stage, inspection has or not the plague of rats and seedbed moisture situation, supplementing in time moisture content;
(7) emerge: treat seed sprouting, record is emerged highly and emergence rate.
Adopt seed seedling-raising method of the present invention, the emergence rate of seed is than high 40% left and right of emergence rate without electromagnetic field processing, and the survival rate of seedling is high, is applicable to very much the needs of implant mass.
Embodiment
Cephalotaxus oliveri is a kind of purposes rare species very widely, and seed, branch, leaf be containing various plants alkali, has desinsection, moistening lung, treatment hemorrhoid, an effect such as long-pending that disappears, and cures mainly parasites disease due to noxious agents produced by various parasites, cough and infantile malnutrition.Branch, leaf can control malignant tumour, to lymphosarcoma, lung are stubborn etc., has a good therapeutic effect.Since the sixties in 20th century, the alkaloid that vegetalization scholar extracts from the branches and leaves of cephalotaxus oliveri, bark, through clinical testing, prove a kind of novel PTS, to the non-lymphatic system leukemia for the treatment of human body, particularly acute myeloblastic leukemia and monokaryon type chronic myeloid leukemia have good curative effect, have anti-cancer, the special medicinal efficacy such as anticancer, for cancer.
But due to the natural breed difficulty of Cephalotaxus oliveri, cause its output not catch up with the needs in market.The present invention, according to the characteristic of Cephalotaxus oliveri, develops the seedling-cultivating method that a kind of emergence rate is higher.
oneplant the seedling-cultivating method of Cephalotaxus oliveri seed:
(1) seed collection: on September 12nd, 2013, collecting location: great Gan town, Dao Zhen county, gathers quantity: approximately 1200, one kilogram of fresh seed, it is mature seed that the gloomy aubergine of being approximately with of exosper is adopted in choosing;
(2) seed treatment; Gathered Cephalotaxus oliveri seed is piled up 5 days, and turning is piled up 5 days again, and exosper is removed in rubbing, and clear water cleans up, and dries; Remove the seed that goes rotten, obtain without going rotten, 1168 of free from admixtures, full seed, the hard bright seed of shell;
(3) electric field treatment: the seed of select is placed on electric field treatment frame and opens field generator, and 10 kilovolts of voltages, process 8 minutes; With the clear water after sterilization is husky, in the ratio of the husky 3:1 of kind, pile up storage 6 months;
(4) soak: on March 28th, 3013, after seed is sifted out, with state's light root-growing agent and carbendazim mixed liquor, invade bubble after 2 hours, pull out and dry, standby;
(5) sowing: by whole good seedbed spread black mulch film, by 20*30 centimetre of nest, line-spacing, with spillikin, punch, seed is sowed in hole, sprinkle fine earth, thin bamboo strips used for weaving reaches arch cover film;
(6) seedbed management: waiting for that between the seeding stage, inspection has or not the plague of rats and seedbed moisture situation, supplementing in time moisture content;
(7) emerge: Mays 22 in 2013 day entry: average 4.8 centimetres of height of seedling, 1096 strains of emerging, emergence rate 91.3%.
Following table is adopt electromagnetic field to process seed and do not adopt electromagnetic field to process the correlation index contrast of seed:
| Title | Seed (grain) | Cycle (moon) | Height of seedling (centimetre) | Emerge and count (strain) | Emergence rate (%) |
| Do not adopt electromagnetic field processing | １２００ | ８ | ３.５ | ６３２ | ５５% |
| Adopt electromagnetic field processing | １２００ | ８ | ４.８ | １０９６ | ９１.３ |
From upper table, can find out, the seed that adopts electromagnetic field to process, its bud ratio is far above the seed that does not pass through electromagnetic field processing.
Claims (1)
1. the seedling-cultivating method of kind of Cephalotaxus oliveri seed, is characterized in that: the concrete steps of described seedling-cultivating method are:
(1) seed collection: select the gloomy approximately Cephalotaxus oliveri seed with aubergine maturation of exosper;
(2) seed treatment; Gathered Cephalotaxus oliveri seed is piled up 5 days, and turning is piled up 5 days again, and exosper is removed in rubbing, and clear water cleans up, and dries; Remove the seed that goes rotten, obtain without mildew and rot, free from admixture, full seed, the hard bright seed of shell;
(3) electric field treatment: the seed of select is placed on electric field treatment frame and opens field generator, and 10 kilovolts of voltages, process 8 minutes; With the clear water after sterilization is husky, in the ratio of the husky 3:1 of kind, pile up storage 6 months;
(4) soak: after the beginning of spring, seed is sifted out, with state's light root-growing agent and carbendazim mixed liquor, invade bubble and pull out and dry after 2 hours, standby;
(5) sowing: by whole good seedbed spread black mulch film, by 20*30 centimetre of nest, line-spacing, with spillikin, punch, seed is sowed in hole, sprinkle fine earth, thin bamboo strips used for weaving reaches arch cover film;
(6) seedbed management: waiting for that between the seeding stage, inspection has or not the plague of rats and seedbed moisture situation, supplementing in time moisture content;
(7) emerge: treat seed sprouting, record is emerged highly and emergence rate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310735024.7A CN103718695B (en) | 2013-12-27 | 2013-12-27 | Method for growing seedlings of cephalotaxus oliveri seeds |
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| CN201310735024.7A CN103718695B (en) | 2013-12-27 | 2013-12-27 | Method for growing seedlings of cephalotaxus oliveri seeds |
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| CN103718695A true CN103718695A (en) | 2014-04-16 |
| CN103718695B CN103718695B (en) | 2015-01-07 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284537A (en) * | 2015-11-06 | 2016-02-03 | 贵州天豪民族药业有限公司 | Seedling method for cephalotaxus fortunei seeds |
| CN105875160A (en) * | 2016-05-04 | 2016-08-24 | 枞阳县熊天然生态农业有限公司 | Photinia fraseri seedling culture method |
| CN108668558A (en) * | 2018-04-19 | 2018-10-19 | 边学静 | A method of abolishing Cephalotaxus mannii seed dormancy |
| CN109526516A (en) * | 2018-12-13 | 2019-03-29 | 怒江景天药业有限公司 | A kind of high mountain cepehalotaxus fortunei seedling breeding method |
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| CN102823357A (en) * | 2012-09-21 | 2012-12-19 | 南京绿宙薄壳山核桃科技有限公司 | Method for accelerating germination of carya illinoensis seeds in late winter and early spring |
| CN103168599A (en) * | 2013-04-07 | 2013-06-26 | 道真仡佬族苗族自治县谷森科技农业开发有限责任公司 | Cephalotaxus fortune cottage seedling raising method |
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| SU1209057A1 (en) * | 1984-07-04 | 1986-02-07 | Московский Ордена Трудового Красного Знамени Институт Инженеров Сельскохозяйственного Производства Им.В.П.Горячкина | Arrangement for preparing seeds for sowing |
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Patent Citations (2)
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| CN102823357A (en) * | 2012-09-21 | 2012-12-19 | 南京绿宙薄壳山核桃科技有限公司 | Method for accelerating germination of carya illinoensis seeds in late winter and early spring |
| CN103168599A (en) * | 2013-04-07 | 2013-06-26 | 道真仡佬族苗族自治县谷森科技农业开发有限责任公司 | Cephalotaxus fortune cottage seedling raising method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284537A (en) * | 2015-11-06 | 2016-02-03 | 贵州天豪民族药业有限公司 | Seedling method for cephalotaxus fortunei seeds |
| CN105875160A (en) * | 2016-05-04 | 2016-08-24 | 枞阳县熊天然生态农业有限公司 | Photinia fraseri seedling culture method |
| CN108668558A (en) * | 2018-04-19 | 2018-10-19 | 边学静 | A method of abolishing Cephalotaxus mannii seed dormancy |
| CN109526516A (en) * | 2018-12-13 | 2019-03-29 | 怒江景天药业有限公司 | A kind of high mountain cepehalotaxus fortunei seedling breeding method |
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