CN103694372A - Method for preparing chitin by use of insects - Google Patents
Method for preparing chitin by use of insects Download PDFInfo
- Publication number
- CN103694372A CN103694372A CN201310646712.6A CN201310646712A CN103694372A CN 103694372 A CN103694372 A CN 103694372A CN 201310646712 A CN201310646712 A CN 201310646712A CN 103694372 A CN103694372 A CN 103694372A
- Authority
- CN
- China
- Prior art keywords
- chitin
- solution
- insect
- hydrogen peroxide
- prepare
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention relates to the biotechnology field and in particular to a method for preparing chitin by use of insects. The method comprises the steps of drying the collected insects in the sun, crushing, adding hydrochloric acid solution with the concentration of 1.0mol/L, soaking for 1-2h, filtering to remove soaking solution, adding cleaning fluid into the filtered powder for cleaning to be neutral, airing, adding the aired powder into 45-65g/L alkaline solution to be soaked according to the weight ratio of 1:10, soaking for 1-2h each time until the soaking solution is colourless to obtain crude chitin, dispersing the crude chitin into water under pH value of 6-11, and adding an oxidizing agent to carry out oxidation and decoloration to obtain the chitin. According to the method, the insects are used as raw materials, chitin contained in the insects is extracted, the process is simple, the obtained product has stable quality, the requirement for aquatic products in industrial production is reduced, the production cost is reduced, natural resource is effectively utilized and environmental pollution is reduced.
Description
Technical field
The present invention relates to biological technical field, is a kind ofly to utilize insect to prepare chitinous method specifically.
Background technology
Chitin has another name called chitin, it is the unique positively charged edibility tunicin of occurring in nature of finding at present, be present in a large number in the cell walls of insect, alga cells film and fungi, nearly 10,000,000,000 tons of annual biosynthesizing amount, is on the earth, to be only second to cellulosic second largest renewable resources.Because chitin is insoluble in diluted acid, diluted alkaline and common organic solvent, limited its widespread use.
The chitin of current suitability for industrialized production and the shell that chitosan mainly comes from shrimp, crab, its preparation method is: in natural crust, add 4～6% hydrochloric acid solns, soak 1～3 day, what have is warmed to 80～90 ℃, what have at room temperature carries out, to remove calcium carbonate and inorganic salt; Then in concentration, be the organism such as deproteination matter in 5～12% NaOH solution, obtain chitin, then with KMnO
4method is decoloured to gained chitin, adds 45～60% alkali lye after decolouring again, and temperature is 70～160 ℃, 6 hours～7 days time, thus deacetylate makes chitosan.There are many defects in this mode of production: (1) is a large amount of mordant acid of apparatus, alkali in preparation process, and the discharge of a large amount of strongly-acids, strong basicity waste liquid causes serious pollution to environment; (2) in acid solution, contain a large amount of CaCl
2, can cause the sclerosis of water quality; (3) in alkali lye, also contain a large amount of protein, the rancid meeting of protein causes ecological deterioration; (4) use KMnO
4to product, decolouring also can produce and contain Mn method
2+and SO
2+waste liquid.In recent years, shrimp shell, crab shell are used as fodder production raw material, caused the shortage of chitin/chitosan raw materials for production, along with going deep into gradually chitin, chitosan and derivative applied research thereof, demand to chitin and chitosan and goods thereof increases substantially, thereby be badly in need of to find new chitin and chitosan resource and production method thereof, with the problem that solves that there is lack of raw materials, and alleviate existing production method pollution on the environment.
Another kind is to extract from fungus sporophores such as the glossy ganoderma of Aphyllophorales polyporaceae, artist's conk, rainbow conk, but these bacterial classifications are limited, cannot satisfy the demands.
Summary of the invention
The object of the invention is the problem existing for above-mentioned technology, provide a kind of and utilize insect to prepare chitinous method, the inventive method can be extracted chitin from insect, makes full use of the natural resources.
For achieving the above object, the technical solution used in the present invention is: a kind ofly utilize insect to prepare chitinous method, it is characterized in that: comprise the steps:
(1) insect of collection is dried, then pulverize, adding concentration is the hydrochloric acid soln of 1.0mol/L, soaks 1～2 hour, then filters, and removes soak solution, filters later powder and adds scavenging solution, cleans to neutral, dries;
(2) powder drying in step (1) is soaked for the ratio of 1:10 adds the basic solution of 45～65g/L by weight, soak 1～2 hour at every turn, until soak solution is colourless, be worth chitin;
(3) under the condition that is 6～11 in pH value, described chitin crude product is scattered in water, adds oxygenant to carry out oxidative decoloration, obtain chitin.
Further: the hydrochloric acid soln amount adding in described step (1) is soaked insect powder completely and is as the criterion reaching.
Further: the basic solution in described step (2) is a kind of in NaOH or KOH or their mixing.
Further: in described step (3), described oxygenant is that mass percentage concentration is 30% aqueous hydrogen peroxide solution; The consumption of water is 5～20 times of described chitin crude product dry weight; Add after aqueous hydrogen peroxide solution, the mass percentage concentration that hydrogen peroxide accounts for hydrogen peroxide, water and chitin crude product gross weight is 3～10%; The number of times of oxidative decoloration is 1～3 time; The time of oxidative decoloration is 0.5～2 hour.
Further: remove agent and described for extracting the ratio of chitinous raw material dry weight, be 10ml:1g.
Useful technique effect of the present invention is: the present invention be take insect as raw material, extracts in insect and contains chitin, and technique is simple, the constant product quality of gained, has lowered the demand to fishery products raw material in industrial production, has lowered production cost, effectively utilize natural resources, reduced environmental pollution.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
(1) insect of collection is dried, then pulverize, adding concentration is the hydrochloric acid soln of 1.0mol/L, and the hydrochloric acid soln amount adding is soaked insect powder completely and is as the criterion reaching, soak 1.5 hours, then filter, remove soak solution, filter later powder and add scavenging solution, clean to neutral, dry;
(2) powder drying in step (1) is added to the NaOH solution soaking of 65g/L by weight for the ratio of 1:10, soak 1 hour at every turn, until soak solution is colourless, be worth chitin;
(3) under the condition that is 8 in pH value, chitin crude product is scattered in water, adds oxygenant to carry out oxidative decoloration, obtain chitin.
Wherein, oxygenant is that mass percentage concentration is 30% aqueous hydrogen peroxide solution; The consumption of water is 10 times of chitin crude product dry weight; Add after aqueous hydrogen peroxide solution, the mass percentage concentration that hydrogen peroxide accounts for hydrogen peroxide, water and chitin crude product gross weight is 7%; The number of times of oxidative decoloration is 2 times; The time of oxidative decoloration is 1 hour; Remove agent with for extracting the ratio of chitinous raw material dry weight, be 10ml:1g.
Embodiment 2
(1) insect of collection is dried, then pulverize, adding concentration is the hydrochloric acid soln of 1.0mol/L, and the hydrochloric acid soln amount adding is soaked insect powder completely and is as the criterion reaching, soak 1 hour, then filter, remove soak solution, filter later powder and add scavenging solution, clean to neutral, dry;
(2) powder drying in step (1) is added to the NaOH solution soaking of 55g/L by weight for the ratio of 1:10, soak 1 hour at every turn, until soak solution is colourless, be worth chitin;
(3) under the condition that is 6 in pH value, described chitin crude product is scattered in water, adds oxygenant to carry out oxidative decoloration, obtain chitin.
Wherein, oxygenant is that mass percentage concentration is 30% aqueous hydrogen peroxide solution; The consumption of water is 5 times of chitin crude product dry weight; Add after aqueous hydrogen peroxide solution, the mass percentage concentration that hydrogen peroxide accounts for hydrogen peroxide, water and chitin crude product gross weight is 3%; The number of times of oxidative decoloration is 1 time; The time of oxidative decoloration is 0.5 hour; Remove agent with for extracting the ratio of chitinous raw material dry weight, be 10ml:1g.
Embodiment 3
(1) insect of collection is dried, then pulverize, adding concentration is the hydrochloric acid soln of 1.0mol/L, and the hydrochloric acid soln amount adding is soaked insect powder completely and is as the criterion reaching, soak 2 hours, then filter, remove soak solution, filter later powder and add scavenging solution, clean to neutral, dry;
(2) powder drying in step (1) is added to the NaOH solution soaking of 35g/L by weight for the ratio of 1:10, soak 1 hour at every turn, until soak solution is colourless, be worth chitin;
(3) under the condition that is 11 in pH value, described chitin crude product is scattered in water, adds oxygenant to carry out oxidative decoloration, obtain chitin.
Wherein, oxygenant is that mass percentage concentration is 30% aqueous hydrogen peroxide solution; The consumption of water is 20 times of chitin crude product dry weight; Add after aqueous hydrogen peroxide solution, the mass percentage concentration that hydrogen peroxide accounts for hydrogen peroxide, water and chitin crude product gross weight is 10%; The number of times of oxidative decoloration is 3 times; The time of oxidative decoloration is 2 hours; Remove agent with for extracting the ratio of chitinous raw material dry weight, be 10ml:1g.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (5)
1. utilize insect to prepare chitinous method, it is characterized in that: comprise the steps:
(1) insect of collection is dried, then pulverize, adding concentration is the hydrochloric acid soln of 1.0mol/L, soaks 1～2 hour, then filters, and removes soak solution, filters later powder and adds scavenging solution, cleans to neutral, dries;
(2) powder drying in step (1) is soaked for the ratio of 1:10 adds the basic solution of 45～65g/L by weight, soak 1～2 hour at every turn, until soak solution is colourless, be worth chitin;
(3) under the condition that is 6～11 in pH value, described chitin crude product is scattered in water, adds oxygenant to carry out oxidative decoloration, obtain chitin.
2. utilize according to claim 1 insect to prepare chitinous method, it is characterized in that: the hydrochloric acid soln amount adding in described step (1) is soaked insect powder completely and is as the criterion reaching.
3. utilize according to claim 1 insect to prepare chitinous method, it is characterized in that: the basic solution in described step (2) is a kind of in NaOH or KOH or their mixing.
4. utilize according to claim 1 insect to prepare chitinous method, it is characterized in that: in described step (3), described oxygenant is that mass percentage concentration is 30% aqueous hydrogen peroxide solution; The consumption of water is 5～20 times of described chitin crude product dry weight; Add after aqueous hydrogen peroxide solution, the mass percentage concentration that hydrogen peroxide accounts for hydrogen peroxide, water and chitin crude product gross weight is 3～10%; The number of times of oxidative decoloration is 1～3 time; The time of oxidative decoloration is 0.5～2 hour.
5. utilize according to claim 4 insect to prepare chitinous method, it is characterized in that: remove agent and described for extracting the ratio of chitinous raw material dry weight, be 10ml:1g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310646712.6A CN103694372A (en) | 2013-12-06 | 2013-12-06 | Method for preparing chitin by use of insects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310646712.6A CN103694372A (en) | 2013-12-06 | 2013-12-06 | Method for preparing chitin by use of insects |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103694372A true CN103694372A (en) | 2014-04-02 |
Family
ID=50356051
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310646712.6A Pending CN103694372A (en) | 2013-12-06 | 2013-12-06 | Method for preparing chitin by use of insects |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103694372A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107257860A (en) * | 2014-12-31 | 2017-10-17 | 昆虫公司 | Chitin, hydrolysate and the mode by means of the enzymatic hydrolysis including being pre-processed with oxidant are used for the method for producing one or more desired products |
| US10995124B2 (en) | 2014-12-31 | 2021-05-04 | Ynsect | Chitin, hydrolysate and production of at least one desired product from insects by means of enzymatic hydrolysis, comprising a combination of steps performed prior to the enzymatic hydrolysis |
| US11432568B2 (en) | 2014-12-31 | 2022-09-06 | Ynsect | Beetle powder |
-
2013
- 2013-12-06 CN CN201310646712.6A patent/CN103694372A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107257860A (en) * | 2014-12-31 | 2017-10-17 | 昆虫公司 | Chitin, hydrolysate and the mode by means of the enzymatic hydrolysis including being pre-processed with oxidant are used for the method for producing one or more desired products |
| US10995124B2 (en) | 2014-12-31 | 2021-05-04 | Ynsect | Chitin, hydrolysate and production of at least one desired product from insects by means of enzymatic hydrolysis, comprising a combination of steps performed prior to the enzymatic hydrolysis |
| US11432568B2 (en) | 2014-12-31 | 2022-09-06 | Ynsect | Beetle powder |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101735337B (en) | Method for preparing chitin and chitosan | |
| CN100398566C (en) | Environment-protection low-consumption process for preparing chitin from shrimp shell and for extracting bioactivity substance | |
| CN101289518B (en) | Method for preparing chitin and process for preparing chitosan by using chitin | |
| CN108299517A (en) | A method of extracting low-molecular weight lignin from biomass | |
| CN102643357B (en) | Method for preparing sodium carboxymethylcellulose by abandoned cotton fabrics | |
| CN106478840B (en) | A kind of preparation method of cross-linked chitosan | |
| CN103694372A (en) | Method for preparing chitin by use of insects | |
| CN105536714A (en) | Heavy metal cellulose adsorbent and preparation method thereof | |
| CN101962618A (en) | Method for harvesting microalgae by using polysaccharide | |
| CN105297511A (en) | Separation method of organic constituent in agricultural waste | |
| CN105435749A (en) | Cellulose modified adsorbent and preparation technology thereof | |
| CN104592417A (en) | Enzyme-process extraction technology for chitin | |
| CN101003581A (en) | Rtechnique for extracting chitin in high molecular weight | |
| Chen et al. | Prospects for bioethanol production from macroalgae | |
| CN103031762B (en) | Method for preparing material rich in cellulose in degradable ionic liquid solvent | |
| CN102718982A (en) | Method for preparing straw cellulose membrane by using wheat straw as raw materials | |
| CN103333271A (en) | Method for producing high-quality calcium alginate | |
| CN107353352A (en) | A kind of preparation method of nano-cellulose, nano-cellulose and water purification film, the preparation method of water purification film | |
| CN105418802A (en) | Method for extracting chitin from shrimp heads and shrimp shells by utilization of organic acids and combined with acidic proteases | |
| CN102827089B (en) | Method for extracting and separating phenazine-1-carboxylic acid (PCA) from fermentation liquor by using flocculence method | |
| CN102617755A (en) | Simple and easy process for preparing chitosan by utilizing shrimp shells or processed residues of shrimp shells | |
| CN103497902A (en) | Cladophora comprehensive utilization technology | |
| CN107540757B (en) | Method for decoloring carrageenan in assistance of enzyme method | |
| CN104905437A (en) | Production process of pineapple leaf fiber fabric | |
| CN105542031B (en) | A kind of extracting method of Euglena polysaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140402 |