CN105418802A - Method for extracting chitin from shrimp heads and shrimp shells by utilization of organic acids and combined with acidic proteases - Google Patents
Method for extracting chitin from shrimp heads and shrimp shells by utilization of organic acids and combined with acidic proteases Download PDFInfo
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- CN105418802A CN105418802A CN201511009161.8A CN201511009161A CN105418802A CN 105418802 A CN105418802 A CN 105418802A CN 201511009161 A CN201511009161 A CN 201511009161A CN 105418802 A CN105418802 A CN 105418802A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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Abstract
Chitin is extracted from shrimp heads and shrimp shells by utilization of organic acids and combined with acidic proteases. Shrimp heads and shrimp shells are subjected to crushing, autolysis filtering and filter residue drying, then an acetic acid solution and acidic proteases are added, a reaction is carried out, then the filter residues are soaked in potassium permanganate and oxalic acid solutions, washing and drying are carried out and chitin is obtained. The reaction conditions are mild, pollution is avoided, the technology is simple, and components of calcium, proteins and the like in the enzymatic hydrolysis solution can be recycled. Because the acidity of organic acids is low, the organic acids are not easy to cause hydrolysis of chitin, and high-molecular chitin can be obtained.
Description
Technical field
The present invention relates to a kind of extracting method of chitin, be specifically related to a kind of method utilizing organic acid associated proteins enzyme chitin extraction from shrimp head shrimp shell.
Background technology
Current China to shrimp products mainly based on outlet, and exported product is based on prawn without head (peeled shrimp), the shrimp head of prawn and shrimp shell account for 30% ~ 40% of whole shrimp quality, containing the protein of more than 10%, the mineral substance of about 5%, the chitin of about 5% and a small amount of fat etc. in shrimp head shrimp shell, wherein chitin is that occurring in nature is only second to cellulosic second largest Biological resources, having a wide range of applications in fields such as food, chemical industry, environmental protection, biotechnology, is a kind of renewable resources having potential using value.
Current chitin extraction mainly adopts acid-base method, microbe fermentation method, utilizes organic acid in conjunction with aspartic protease extraction method in addition.Although acid-base method chitin extraction cost is lower, technique is simple, seriously polluted, and degradation of chitin is more serious; Microbe fermentation method reaction conditions is gentle, environmentally friendly, can obtain the chitin of higher molecular weight, and the composition such as protein, astaxanthin also can be recycled, but this method length consuming time, and needing the equipment such as fermentor tank, technique is comparatively complicated.
Summary of the invention
The object of this invention is to provide a kind of method utilizing organic acid associated proteins enzyme chitin extraction from shrimp head shrimp shell, the method reaction conditions is gentle, and technique is simple.
In order to achieve the above object, the technical scheme that the present invention takes is:
Utilize a method for organic acid associated proteins enzyme chitin extraction from shrimp head shrimp shell, comprise the steps:
(1) by after broken for shrimp head shrimp shell meal, be placed in 35 ~ 45 DEG C of shaking table self-dissolving 3h, filtered through gauze, it is for subsequent use that filter residue distilled water cleans up post-drying;
(2) the shrimp head shrimp shell of a certain amount of oven dry is taken, add acetic acid solution, again with a small amount of acetic acid adjust ph to 2.5 ~ 4.0 when obviously weakening to bubble generation, then a certain amount of aspartic protease is added, in 35 ~ 45 DEG C of shaking table reaction 40 ~ 53h, period continuous adjust ph, maintain 2.5 ~ 4.0 constant;
(3) filter after completion of the reaction, and clean filter residue with distilled water, until washings is colourless, then filter residue 75 ~ 85min is soaked with potassium permanganate solution in 48 ~ 58 DEG C, soak with oxalic acid solution again after filtration, to filter residue color is constant, clean post-drying.
As specializing, the amount ratio of described shrimp head and the consumption of shrimp shell and acetic acid solution is 1:10 ~ 30, and the massfraction of acetic acid solution is 15% ~ 25%.
Preferably, the amount ratio of described shrimp head and the consumption of shrimp shell and acetic acid solution is 1:20, and the massfraction of acetic acid solution is 20%.
As specializing, the consumption of described aspartic protease is 1% ~ 3% of shrimp head shrimp shell consumption.
Preferably, the consumption of described aspartic protease is 2% of shrimp head shrimp shell consumption.
As specializing, the massfraction of described potassium permanganate solution is 1%, and the massfraction of described oxalic acid solution is 1%.
Compared with prior art, beneficial effect of the present invention:
The present invention utilizes organic acid in conjunction with aspartic protease chitin extraction from shrimp head shrimp shell, reaction conditions is gentle, avoid pollution, technique is simple, and the composition also recoverable such as calcium and protein in enzymolysis solution, because organic acid acidity is lower, not easily cause chitin to be hydrolyzed, polymer chitin can be obtained.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
(1) by after broken for shrimp head shrimp shell meal, be placed in 35 DEG C of shaking table self-dissolving 3h, filtered through gauze, it is for subsequent use that filter residue distilled water cleans up post-drying;
(2) take the shrimp head shrimp shell that 5g is dried, add 15% acetic acid solution of 50mL, again with a small amount of acetic acid adjust ph to 2.5 when obviously weakening to bubble generation, then the aspartic protease of 0.1g is added, in 35 DEG C of shaking tables reaction 40h, period continuous adjust ph, maintain 2.5 constant;
(3) filter after completion of the reaction, and clean filter residue with distilled water, until washings is colourless, then be that the potassium permanganate solution of 1% is in 50 DEG C of immersion filter residue 75min with massfraction, after filtration again with massfraction be 1% oxalic acid solution soak, to filter residue color is constant, clean post-drying.
Embodiment 2
(1) by after broken for shrimp head shrimp shell meal, be placed in 40 DEG C of shaking table self-dissolving 3h, filtered through gauze, it is for subsequent use that filter residue distilled water cleans up post-drying;
(2) take the shrimp head shrimp shell that 8g is dried, add 18% acetic acid solution of 120mL, again with a small amount of acetic acid adjust ph to 3.0 when obviously weakening to bubble generation, then the aspartic protease of 0.12g is added, in 40 DEG C of shaking tables reaction 45h, period continuous adjust ph, maintain 3.0 constant;
(3) filter after completion of the reaction, and clean filter residue with distilled water, until washings is colourless, then be that the potassium permanganate solution of 1% is in 48 DEG C of immersion filter residue 80min with massfraction, after filtration again with massfraction be 1% oxalic acid solution soak, to filter residue color is constant, clean post-drying.
Embodiment 3
(1) by after broken for shrimp head shrimp shell meal, be placed in 45 DEG C of shaking table self-dissolving 3h, filtered through gauze, it is for subsequent use that filter residue distilled water cleans up post-drying;
(2) take the shrimp head shrimp shell that 10g is dried, add 20% acetic acid solution of 200mL, again with a small amount of acetic acid adjust ph to 4.0 when obviously weakening to bubble generation, then the aspartic protease of 0.2g is added, in 45 DEG C of shaking tables reaction 48h, period continuous adjust ph, maintain 4.0 constant;
(3) after completion of the reaction, take out and filter, and clean filter residue with distilled water, until washings is colourless, then massfraction is that the potassium permanganate solution of 1% is in 50 DEG C of immersion filter residue 82min, after filtration again with massfraction be 1% oxalic acid solution soak, to filter residue color is constant, clean post-drying.
Embodiment 4
(1) by after broken for shrimp head shrimp shell meal, be placed in 38 DEG C of shaking table self-dissolving 3h, filtered through gauze, it is for subsequent use that filter residue distilled water cleans up post-drying;
(2) take the shrimp head shrimp shell that 12g is dried, add 22% acetic acid solution of 300mL, again with a small amount of acetic acid adjust ph to 3.5 when obviously weakening to bubble generation, then the aspartic protease of 0.3g is added, in 38 DEG C of shaking tables reaction 50h, period continuous adjust ph, maintain 3.5 constant;
(3) after completion of the reaction, take out and filter, and clean filter residue with distilled water, until washings is colourless, then be that the potassium permanganate solution of 1% is in 55 DEG C of immersion filter residue 85min with massfraction, after filtration again with massfraction be 1% oxalic acid solution soak, to filter residue color is constant, clean post-drying.
Embodiment 5
(1) by after broken for shrimp head shrimp shell meal, be placed in 43 DEG C of shaking table self-dissolving 3h, filtered through gauze, it is for subsequent use that filter residue distilled water cleans up post-drying;
(2) take the shrimp head shrimp shell that 15g is dried, add 25% acetic acid solution of 450mL, again with a small amount of acetic acid adjust ph to 3.8 when obviously weakening to bubble generation, then the aspartic protease of 0.45g is added, in 43 DEG C of shaking tables reaction 53h, period continuous adjust ph, maintain 3.8 constant;
(3) after completion of the reaction, take out and filter, and clean filter residue with distilled water, until washings is colourless, then be that the potassium permanganate solution of 1% is in 58 DEG C of immersion filter residue 80min with massfraction, after filtration again with massfraction be 1% oxalic acid solution soak, to filter residue color is constant, clean post-drying.
The chitin that embodiment 1,2,3,4,5 is extracted is carried out the mensuration of ash content and protein content, result is as shown in table 1 below.
Table 1
| Performance test | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 |
| Ash oontent/% | 1.78 | 1.82 | 1.91 | 1.88 | 1.86 |
| Protein content/% | 5.96 | 6.34 | 6.52 | 6.41 | 6.45 |
The above, it is only present pre-ferred embodiments, not technical scope of the present invention is imposed any restrictions, thus every above embodiment is done according to technical spirit of the present invention any trickle amendment, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (6)
1. utilize a method for organic acid associated proteins enzyme chitin extraction from shrimp head shrimp shell, it is characterized in that, comprise the steps:
(1) by after broken for shrimp head shrimp shell meal, be placed in 35 ~ 45 DEG C of shaking table self-dissolving 3h, filtered through gauze, it is for subsequent use that filter residue distilled water cleans up post-drying;
(2) the shrimp head shrimp shell of a certain amount of oven dry is taken, add acetic acid solution, again with a small amount of acetic acid adjust ph to 2.5 ~ 4.0 when obviously weakening to bubble generation, then a certain amount of aspartic protease is added, in 35 ~ 45 DEG C of shaking table reaction 40 ~ 53h, period continuous adjust ph, maintain 2.5 ~ 4.0 constant;
(3) filter after completion of the reaction, and clean filter residue with distilled water, until washings is colourless, then filter residue 75 ~ 85min is soaked with potassium permanganate solution in 48 ~ 58 DEG C, soak with oxalic acid solution again after filtration, to filter residue color is constant, clean post-drying.
2. the method utilizing organic acid associated proteins enzyme chitin extraction from shrimp head shrimp shell according to claim 1, it is characterized in that, the amount ratio of described shrimp head and the consumption of shrimp shell and acetic acid solution is 1:10 ~ 30, and the massfraction of acetic acid solution is 15% ~ 25%.
3. the method utilizing organic acid associated proteins enzyme chitin extraction from shrimp head shrimp shell according to claim 1 and 2, is characterized in that, the amount ratio of described shrimp head and the consumption of shrimp shell and acetic acid solution is 1:20, and the massfraction of acetic acid solution is 20%.
4. the method utilizing organic acid associated proteins enzyme chitin extraction from shrimp head shrimp shell according to claim 1, is characterized in that, the consumption of described aspartic protease is 1% ~ 3% of shrimp head shrimp shell consumption.
5. the method utilizing organic acid associated proteins enzyme chitin extraction from shrimp head shrimp shell according to claim 1 or 4, is characterized in that, the consumption of described aspartic protease is 2% of shrimp head shrimp shell consumption.
6. the method utilizing organic acid associated proteins enzyme chitin extraction from shrimp head shrimp shell according to claim 1, is characterized in that, the massfraction of described potassium permanganate solution is 1%, and the massfraction of described oxalic acid solution is 1%.
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| CN201511009161.8A CN105418802A (en) | 2015-12-29 | 2015-12-29 | Method for extracting chitin from shrimp heads and shrimp shells by utilization of organic acids and combined with acidic proteases |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106188344A (en) * | 2016-08-17 | 2016-12-07 | 吴冰滢 | A kind of extracting method of high-purity chitin in high molecular weight |
| CN106343250A (en) * | 2016-08-25 | 2017-01-25 | 湖北伟望农牧科技发展集团有限公司 | Fish fry feed and preparation method thereof |
| CN106810623A (en) * | 2016-12-02 | 2017-06-09 | 马卡*** | A kind of chitin and its shitosan and application |
| CN106967772A (en) * | 2017-03-24 | 2017-07-21 | 中国水产科学研究院长江水产研究所 | A kind of method that utilization magnetic immobilized alkali protease extracts shrimp shell albumen |
| CN110643668A (en) * | 2019-10-29 | 2020-01-03 | 海南大学 | Method for extracting chitin from shrimp shells by utilizing microbial fermentation |
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| CN101904405A (en) * | 2010-07-05 | 2010-12-08 | 许淑贤 | Method for extracting protein and chitin from shrimp heads and shrimp shells by using edible acid |
| CN102086464A (en) * | 2009-12-02 | 2011-06-08 | 林大昌 | Method for preparing chitin |
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2015
- 2015-12-29 CN CN201511009161.8A patent/CN105418802A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102086464A (en) * | 2009-12-02 | 2011-06-08 | 林大昌 | Method for preparing chitin |
| CN101904405A (en) * | 2010-07-05 | 2010-12-08 | 许淑贤 | Method for extracting protein and chitin from shrimp heads and shrimp shells by using edible acid |
Non-Patent Citations (1)
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106188344A (en) * | 2016-08-17 | 2016-12-07 | 吴冰滢 | A kind of extracting method of high-purity chitin in high molecular weight |
| CN106188344B (en) * | 2016-08-17 | 2018-09-07 | 吴冰滢 | A kind of extracting method of high-purity chitin in high molecular weight |
| CN106343250A (en) * | 2016-08-25 | 2017-01-25 | 湖北伟望农牧科技发展集团有限公司 | Fish fry feed and preparation method thereof |
| CN106810623A (en) * | 2016-12-02 | 2017-06-09 | 马卡*** | A kind of chitin and its shitosan and application |
| CN106967772A (en) * | 2017-03-24 | 2017-07-21 | 中国水产科学研究院长江水产研究所 | A kind of method that utilization magnetic immobilized alkali protease extracts shrimp shell albumen |
| CN110643668A (en) * | 2019-10-29 | 2020-01-03 | 海南大学 | Method for extracting chitin from shrimp shells by utilizing microbial fermentation |
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Application publication date: 20160323 |