CN104592417A - Enzyme-process extraction technology for chitin - Google Patents
Enzyme-process extraction technology for chitin Download PDFInfo
- Publication number
- CN104592417A CN104592417A CN201410716450.0A CN201410716450A CN104592417A CN 104592417 A CN104592417 A CN 104592417A CN 201410716450 A CN201410716450 A CN 201410716450A CN 104592417 A CN104592417 A CN 104592417A
- Authority
- CN
- China
- Prior art keywords
- chitin
- parts
- shrimp
- extraction processes
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention belongs to the technical field of natural product separation extraction, and concretely discloses an enzyme-process extraction technology for chitin. The technology comprises the following steps: drying and crushing recovered shrimp and crab shells for usage; adding water with the amount being 6 times by weight of the shrimp and crab powder, performing ultrasonic processing, and standing for swelling; then adding compound-enzyme hydrolyzed protein according to 2-5% of the weight of the shrimp and crab powder, and performing coarse filtration, centrifugal separation and fine filtration, so as to obtain a clean shell powder; and adding a citric acid solution into the obtained clean shell powder for immersing and removing calcium, and performing filtration, cleaning and drying, so as to obtain the chitin product. The compound enzyme is composed of the following compositions in parts by weight: 3-10 parts of trypsin, 10-12 parts of papain, 5-8 parts of alkali protease and 20-30 parts of bacillus subtilis protease. Through ultrasonic pretreatment and proportioning of specific proteases, the protein recovery rate is improved, produced chitin is high in quality, the usage amount of an acid solution in subsequent process of performing acid processing on chitin is substantially reduced, and comprehensive utilization of resource is facilitated.
Description
Technical field
The invention belongs to Separation of Natural Products extractive technique field, relate to a kind of with the Technology of Crustacean processing waste for Raw material processing production chitin, more specifically, relate to a kind of Extraction Processes of chitin.
Background technology
China's crust aboundresources, cultivation, the amount of finish of Crustacean are huge, have a large amount of processing wastes simultaneously, and only crab, shrimp waste reach more than 6000 tons every year, and in Crustacean processing waste, chitin (Chitin) content is up to 20 ~ 25%.At present, it is found that chitin and derivative thereof have many premium propertiess, purposes is very extensive, has application in industries such as medicine, agricultural, environmental protection, food, weaving, printing and dyeing, plastics, color films.Particularly in medicine and health products trade, application prospect is more wide.
At present, there are the following problems to utilize the utilization of Crustacean processing waste: processing technology is backward, cost is high thus cause the wasting of resources, and using strong acid, highly basic to cause again severe contamination to produce high density, organic high-salinity wastewater in a large number, its pollutant component comprises a large amount of CaCl
2, the salt such as NaCl, a large amount of animal proteinum (shrimp, crab glutelin) and degraded product, shrimp and crab shells pigment, grease, the impurity that raw material itself is brought into, a small amount of chitin and chitosan etc.To produce 1 ton of chitin daily, 10 ~ 150,000 tons of production wastes to be discharged to environment water every year.At present in the improvement of chitin production waste, take simple end treatment mode, disposablely to have high input, running expense is high, and is difficult to realize qualified discharge.Therefore, adopt and clean technology, utilize the processing method of Enzymatic Extraction chitin causing research and development and the discussion of people.
When utilizing Enzymatic Extraction chitin, little to the quality influence of protein and chitin, production process safety, environmental pollution is few.But mostly adopt single proteolytic enzyme to be hydrolyzed at present, hydrolysis efficiency is far below acid-base method, and protein recovery is not high.
Summary of the invention
The object of the invention is to overcome the shortcoming existed in prior art processes, a kind of Extraction Processes of chitin is provided, utilizes multiplex-enzyme extraction chitin and reclaim albumen, simultaneously the auxiliary processing method having ultrasonic pretreatment, to increase extraction efficiency, increase added value of product.
The present invention is achieved through the following technical solutions above-mentioned purpose.
The invention provides a kind of Extraction Processes of chitin, comprise the steps: the shrimp and crab shells of recovery to dry, pulverize for subsequent use; Add water by 6 times of shrimp and crab shells powder weight, and by ultrasonication, then leave standstill swelling; Add combinative enzyme hydrolysis albumen by the 2-5% of shrimp and crab shells powder weight again, then obtain clean shell powder through coarse filtration, centrifugation, smart leaching; By after the clean shell powder adding citric acid immersion bubble decalcification that obtains, then after filtration, clean, dry to obtain chitin product; Described prozyme is made up of each number of following weight proportion: trypsinase 3-10 part, papoid 10-12 part, Sumizyme MP 5-8 part, Validase TSP Concentrate II 20-30 part.
The present invention finds, by the proportioning of ultrasonic pretreatment and particular types proteolytic enzyme, substantially increase the rate of recovery of protein, and it is little to the qualitative effects of chitin when adopting enzymatic treatment, the chitin quality of producing is high, steady quality, in the acid treatment process of follow-up chitin, the consumption of acid solution significantly reduces, and is beneficial to the comprehensive utilization of resource.
Preferably, described ultrasonication power is 250-500W, time 30-60min.
Preferably, described standing swelling treatment time 30-60min.
Preferably, described prozyme is made up of each number of following weight proportion: 8 parts, trypsinase, papoid 10 parts, Sumizyme MP 7 parts, Validase TSP Concentrate II 22-25 part.
Preferably, the weight percent concentration of the citric acid solution used in chitin preparation process is 10-18%.
Preferably, described citric acid solution soak time is 3-4.5 hour.
Preferably, also obtain smart filtrate and thick fatty oil through coarse filtration, centrifugation, smart leaching while obtaining clean shell powder, smart filtrate is as the raw material preparing protein powder product, and thick fatty oil is as the raw material of preparation shrimp crab oil.
The present invention compared with prior art, there is following beneficial effect: the present invention passes through the proportioning of ultrasonic pretreatment and particular types proteolytic enzyme, substantially increase the rate of recovery of protein, and it is little to the qualitative effects of chitin when adopting enzymatic treatment, the chitin quality of producing is high, steady quality, in the acid treatment process of follow-up chitin, the consumption of acid solution significantly reduces, and is beneficial to the comprehensive utilization of resource.
Embodiment
Below in conjunction with specific embodiment the present invention made and elaborating further, but embodiment does not limit in any form the present invention.Unless stated otherwise, the present invention adopts reagent, method and apparatus are the art conventional reagent, method and apparatus.
embodiment 1
With shrimp shell for raw material preparing product, dry at first shrimp shell being placed in 85 DEG C, more dry shrimp shell meal is broken to 40 orders, drop in beaker and add 6 times of water mixing, put into ultrasonoscope, conditioning instrumentation power 250W, process 60min.Taking-up after supersound process completes, find that mixture solution becomes muddy, shrimp shell structure changes.Then, mixture solution is made to leave standstill swelling 1 hour at normal temperatures; Then, be heated to 50 DEG C, according to the weight of input shrimp shell meal raw material by 2% weight percent add prozyme, described prozyme is made up of 8 parts of trypsinase, 10 parts of papoids, 7 parts of Sumizyme MPs and 22 parts of Validase TSP Concentrate IIs.Adjusted to ph is about 7.5, and stirring reaction, after 2 hours, namely obtains smart filtrate and clean shrimp shell meal with plate-and-frame filter press press filtration; By smart filtrate centrifugation, upper liquid and thick grease, lower floor's remaining liq is Hydrolyzed protein liquid, then after decolouring and the filter of second time essence, the desalination of upper Sephadex G-10 post, then protein powder product is namely obtained after vacuum concentration, spraying dry; After through first time, the clean shell powder that obtains of the filtering separation citric acid solution that adds 11% soaks decalcification in 3 hours, repeated hydrogenation sodium oxide is neutralized to neutrality, then after filtration, cleans, dries to obtain chitin product; The thick grease of upper liquid gone out from the centrifugation of enzyme liquid can be obtained shrimp sauce product after refining.In protein powder product, protein content is 71.3%, and in chitin product, ash content is 0.48%.
embodiment 2
With shrimp shell for raw material preparing product, dry at first shrimp shell being placed in 75 DEG C, more dry shrimp shell meal is broken to 60 orders, drop in beaker and add 6 times of water mixing, put into ultrasonoscope, conditioning instrumentation power 350W, process 60min.Taking-up after supersound process completes, find that mixture solution is just muddy, shrimp shell structure changes.Then, mixture solution is made to leave standstill swelling 30min at normal temperatures; Then, be heated to 50 DEG C, according to the weight of input shrimp shell meal raw material by 2.3% weight percent add prozyme, described prozyme is made up of 3 parts of trypsinase, 12 parts of papoids, 7 parts of Sumizyme MPs and 25 parts of Validase TSP Concentrate IIs.Adjusted to ph is about 7.8, and stirring reaction, after 2 hours, namely obtains smart filtrate and clean shrimp shell meal with plate-and-frame filter press press filtration; By smart filtrate centrifugation, upper liquid and thick grease, lower floor's remaining liq is Hydrolyzed protein liquid, then after decolouring and the filter of second time essence, the desalination of upper Sephadex G-10 post, then protein powder product is namely obtained after vacuum concentration, spraying dry; After through first time, the clean shell powder that obtains of the filtering separation citric acid solution that adds 10% soaks decalcification in 3 hours, repeated hydrogenation sodium oxide is neutralized to neutrality, then after filtration, cleans, dries to obtain chitin product; The thick grease of upper liquid gone out from the centrifugation of enzyme liquid can be obtained shrimp sauce product after refining.In protein powder product, protein content is 70%, and in chitin product, ash content is 0.39%.
embodiment 3
Step is identical with embodiment 2 with method, and with crab shell for raw material, its quality product is identical.
comparative example 1
With shrimp shell for raw material preparing product, dry at first shrimp shell being placed in 85 DEG C, more dry shrimp shell meal is broken to 40 orders, drop in beaker and add 6 times of water mixing, put into ultrasonoscope, conditioning instrumentation power 200W, process 60min.Taking-up after supersound process completes, find that mixture solution is just muddy, shrimp shell structure changes.Then, mixture solution is made to leave standstill swelling 1 hour at normal temperatures; Then, be heated to 50 DEG C, according to the weight of input shrimp shell meal raw material by 2% weight percent add prozyme, described prozyme is made up of 4 parts of trypsinase, 10 parts of papoids, 7 parts of Sumizyme MPs and 12 parts of Validase TSP Concentrate IIs.Adjusted to ph is about 7.5, and stirring reaction, after 2 hours, namely obtains smart filtrate and clean shrimp shell meal with plate-and-frame filter press press filtration; By smart filtrate centrifugation, upper liquid and thick grease, lower floor's remaining liq is Hydrolyzed protein liquid, then after decolouring and the filter of second time essence, the desalination of upper Sephadex G-10 post, then protein powder product is namely obtained after vacuum concentration, spraying dry; After through first time, the clean shell powder that obtains of the filtering separation citric acid solution that adds 11% soaks decalcification in 3 hours, repeated hydrogenation sodium oxide is neutralized to neutrality, then after filtration, cleans, dries to obtain chitin product; The thick grease of upper liquid gone out from the centrifugation of enzyme liquid can be obtained shrimp sauce product after refining.In protein powder product, protein content is 54.6%, and in chitin product, ash content is 1.95%.
Claims (7)
1. an Extraction Processes for chitin, is characterized in that, comprise the steps: the shrimp and crab shells of recovery to dry, pulverize for subsequent use; Add water by 6 times of shrimp and crab shells powder weight, and by ultrasonication, then leave standstill swelling; Add combinative enzyme hydrolysis albumen by the 2-5% of shrimp and crab shells powder weight again, then obtain clean shell powder through coarse filtration, centrifugation, smart leaching; By after the clean shell powder adding citric acid immersion bubble decalcification that obtains, then after filtration, clean, dry to obtain chitin product; Described prozyme is made up of each number of following weight proportion: trypsinase 3-10 part, papoid 10-12 part, Sumizyme MP 5-8 part, Validase TSP Concentrate II 20-30 part.
2. the Extraction Processes of chitin according to claim 1, is characterized in that, described ultrasonication power is 250-500W, time 30-60min.
3. the Extraction Processes of chitin according to claim 2, is characterized in that, described standing swelling treatment time 30-60min.
4. the Extraction Processes of chitin according to claim 3, it is characterized in that, described prozyme is made up of each number of following weight proportion: 8 parts, trypsinase, papoid 10 parts, Sumizyme MP 7 parts, Validase TSP Concentrate II 22-25 part.
5. the Extraction Processes of chitin according to claim 4, is characterized in that, the weight percent concentration of the citric acid solution used in chitin preparation process is 10-18%.
6. the Extraction Processes of chitin according to claim 5, is characterized in that, described citric acid solution soak time is 3-4.5 hour.
7. the Extraction Processes of chitin according to claim 1, it is characterized in that, also obtain smart filtrate and thick fatty oil while obtaining clean shell powder through coarse filtration, centrifugation, smart leaching, smart filtrate is as the raw material preparing protein powder product, and thick fatty oil is as the raw material of preparation shrimp crab oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410716450.0A CN104592417A (en) | 2014-12-02 | 2014-12-02 | Enzyme-process extraction technology for chitin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410716450.0A CN104592417A (en) | 2014-12-02 | 2014-12-02 | Enzyme-process extraction technology for chitin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104592417A true CN104592417A (en) | 2015-05-06 |
Family
ID=53118506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410716450.0A Pending CN104592417A (en) | 2014-12-02 | 2014-12-02 | Enzyme-process extraction technology for chitin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104592417A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105085707A (en) * | 2015-05-13 | 2015-11-25 | 浙江海洋学院 | Production method of chitin by using shrimp shell |
| CN105754015A (en) * | 2016-02-29 | 2016-07-13 | 苏州市贝克生物科技有限公司 | Method for extracting chitin from oyster shell |
| CN106496362A (en) * | 2016-12-12 | 2017-03-15 | 钦州学院 | The extracting method of chitin in a kind of Carapax Eriocheir sinensis |
| CN106977623A (en) * | 2017-04-13 | 2017-07-25 | 广东人为峰健康管理有限公司 | A kind of method of chitin extraction in fly maggot from drosophila |
| CN108707639A (en) * | 2018-06-09 | 2018-10-26 | 浙江亿丰海洋生物制品有限公司 | A kind of preparation method of small oppossum shrimp enzymolysis small peptide |
| CN109628525A (en) * | 2018-12-17 | 2019-04-16 | 上海冠硕生物科技有限公司 | A kind of technique that enzymatic hydrolysis chitin prepares N-acetylglucosamine |
| CN109647792A (en) * | 2018-12-27 | 2019-04-19 | 温州科技职业学院 | A kind of method of the lower shrimp shell desalination of ultrasound |
-
2014
- 2014-12-02 CN CN201410716450.0A patent/CN104592417A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105085707A (en) * | 2015-05-13 | 2015-11-25 | 浙江海洋学院 | Production method of chitin by using shrimp shell |
| CN105754015A (en) * | 2016-02-29 | 2016-07-13 | 苏州市贝克生物科技有限公司 | Method for extracting chitin from oyster shell |
| CN106496362A (en) * | 2016-12-12 | 2017-03-15 | 钦州学院 | The extracting method of chitin in a kind of Carapax Eriocheir sinensis |
| CN106977623A (en) * | 2017-04-13 | 2017-07-25 | 广东人为峰健康管理有限公司 | A kind of method of chitin extraction in fly maggot from drosophila |
| CN108707639A (en) * | 2018-06-09 | 2018-10-26 | 浙江亿丰海洋生物制品有限公司 | A kind of preparation method of small oppossum shrimp enzymolysis small peptide |
| CN109628525A (en) * | 2018-12-17 | 2019-04-16 | 上海冠硕生物科技有限公司 | A kind of technique that enzymatic hydrolysis chitin prepares N-acetylglucosamine |
| CN109647792A (en) * | 2018-12-27 | 2019-04-19 | 温州科技职业学院 | A kind of method of the lower shrimp shell desalination of ultrasound |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104592417A (en) | Enzyme-process extraction technology for chitin | |
| JP7422236B2 (en) | Method for producing clam active peptide | |
| CN105325801B (en) | Preparation method of sea cucumber intestinal peptide | |
| CN104140474B (en) | A kind of method of utility in comprehensive utilizing shrimp Eriocheir sinensis skin | |
| CN105063153B (en) | A kind of preparation method of food-grade low salt ocean fish oligopeptide powder | |
| CN105348411B (en) | A method of preparing β-chitin using squid sector bone | |
| CN102308903B (en) | Industrialized production method of corn polypeptide | |
| CN103467624B (en) | A kind of extracting method of heparin sodium crude | |
| CN100441693C (en) | Process for preparing chitin and chitosan | |
| CN103805071B (en) | Extraction method of deep-sea fish gelatin | |
| CN103755834B (en) | A kind of method of preparing active peptide powder and chitin from shrimp crab accessory substance | |
| KR101904019B1 (en) | Alginic acid materialization method using waste seaweed and enzymes | |
| CN106319014A (en) | Extracting method for micro-molecule deep-sea fish polypeptides | |
| CN104292364A (en) | Method for extracting bioactive substances from eggshell membrane | |
| CN103613637A (en) | Method of preparing proteins, chitin and chitosan from degreased shrimp meals of euphausia superba | |
| CN105111330A (en) | Process utilizing enzymatic removing impurity to prepare tussah pupa skin chitosan | |
| CN101869169B (en) | Method for preparing fish oligopeptide from gurry by combining fermentation and membrane technology | |
| CN106035980B (en) | A method of dried porcine saluble is produced using enzymatic isolation method heparin adsorption raffinate | |
| CN103627768A (en) | Fish skin collagen bioactive small peptide and preparation method thereof | |
| CN103750258A (en) | Method for preparing flavored yeast extracts and polysaccharides from waste beer yeasts | |
| CN106749763B (en) | A method of high-purity crust being prepared from shrimp shell meal using ionic liquid | |
| CN101275155A (en) | Clean production method for zitan and chitin | |
| CN100422217C (en) | Process for preparing pectin and heavy metallic ion adsorber by soybean peel combined production | |
| CN105420329A (en) | Method for making polypeptide chelated calcium with oil-peeled wood frog residual body as raw material | |
| CN105154506B (en) | A kind of food-grade low salt ocean fish oligopeptide powder and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150506 |